The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 980 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 446 |
| AatII | GACGTC | 1 cut(s) | 333 |
| AccB1I | GGYRCC | 2 cut(s) | 281, 1054 |
| AccI | GTMKAC | 2 cut(s) | 2158, 2655 |
| AccII | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| AccIII | TCCGGA | 1 cut(s) | 2578 |
| AcoI | YGGCCR | 1 cut(s) | 2523 |
| AcsI | RAATTY | 2 cut(s) | 2506, 2834 |
| AcuI | CTGAAG | 5 cut(s) | 374, 1455, 2378, 2448, 2486 |
| AcyI | GRCGYC | 2 cut(s) | 330, 779 |
| AfaI | GTAC | 4 cut(s) | 572, 716, 968, 1591 |
| AfeI | AGCGCT | 1 cut(s) | 1476 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 224, 241, 358, 436, 691, 717 |
| AflII | CTTAAG | 1 cut(s) | 1157 |
| AflIII | ACRYGT | 1 cut(s) | 1389 |
| AjiI | CACGTC | 1 cut(s) | 1483 |
| AjnI | CCWGG | 3 cut(s) | 261, 696, 1580 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 206, 238, 2498, 2530 |
| AleI | CACNNNNGTG | 1 cut(s) | 1427 |
| Alw21I | GWGCWC | 4 cut(s) | 666, 1206, 1424, 1757 |
| Alw26I | GTCTC | 5 cut(s) | 728, 1907, 2026, 2620, 2654 |
| Ama87I | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| Aor13HI | TCCGGA | 1 cut(s) | 2578 |
| Aor51HI | AGCGCT | 1 cut(s) | 1476 |
| AoxI | GGCC | 6 cut(s) | 63, 360, 1772, 2223, 2523, 3082 |
| ApeKI | GCWGC | 9 cut(s) | 721, 1073, 1076, 1525, 1549, 1814, 2029, 2191, 2918 |
| ApoI | RAATTY | 2 cut(s) | 2506, 2834 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 492, 524 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1962, 2499 |
| AspLEI | GCGC | 7 cut(s) | 103, 684, 752, 754, 836, 838, 1477 |
| AsuC2I | CCSGG | 1 cut(s) | 2198 |
| AsuHPI | GGTGA | 7 cut(s) | 400, 965, 1625, 2135, 2546, 2974, 3055 |
| AvaI | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| AvaII | GGWCC | 7 cut(s) | 265, 439, 508, 694, 790, 1794, 2317 |
| AxyI | CCTNAGG | 1 cut(s) | 1410 |
| BaeGI | GKGCMC | 2 cut(s) | 284, 1213 |
| BalI | TGGCCA | 1 cut(s) | 2525 |
| BamHI | GGATCC | 1 cut(s) | 199 |
| BanI | GGYRCC | 2 cut(s) | 281, 1054 |
| BbsI | GAAGAC | 3 cut(s) | 1250, 1331, 1755 |
| Bbv12I | GWGCWC | 4 cut(s) | 666, 1206, 1424, 1757 |
| BbvI | GCAGC | 9 cut(s) | 708, 1063, 1085, 1512, 1536, 1801, 2016, 2203, 2905 |
| BceAI | ACGGC | 2 cut(s) | 995, 2789 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 658, 692, 2094, 2128 |
| BciT130I | CCWGG | 3 cut(s) | 263, 698, 1582 |
| BciVI | GTATCC | 2 cut(s) | 1549, 2999 |
| BclI | TGATCA | 1 cut(s) | 2013 |
| BcnI | CCSGG | 1 cut(s) | 2198 |
| BcoDI | GTCTC | 5 cut(s) | 728, 1907, 2026, 2620, 2654 |
| BfaI | CTAG | 4 cut(s) | 1005, 1767, 2147, 2573 |
| BfmI | CTRYAG | 1 cut(s) | 937 |
| BfoI | RGCGCY | 1 cut(s) | 1478 |
| BfrI | CTTAAG | 1 cut(s) | 1157 |
| BfuI | GTATCC | 2 cut(s) | 1549, 2999 |
| BglII | AGATCT | 1 cut(s) | 152 |
| Bme1390I | CCNGG | 4 cut(s) | 263, 698, 1582, 2198 |
| Bme18I | GGWCC | 7 cut(s) | 265, 439, 508, 694, 790, 1794, 2317 |
| BmeT110I | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| BmgBI | CACGTC | 1 cut(s) | 1483 |
| BmiI | GGNNCC | 7 cut(s) | 201, 283, 596, 792, 1033, 1056, 1795 |
| BmrFI | CCNGG | 4 cut(s) | 263, 698, 1582, 2198 |
| BmrI | ACTGGG | 1 cut(s) | 2045 |
| BmsI | GCATC | 6 cut(s) | 1441, 1711, 2109, 2359, 2750, 2944 |
| BmuI | ACTGGG | 1 cut(s) | 2045 |
| BpiI | GAAGAC | 3 cut(s) | 1250, 1331, 1755 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 573, 605, 1231, 1263 |
| BpmI | CTGGAG | 2 cut(s) | 539, 2364 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1205, 2220 |
| BpuEI | CTTGAG | 8 cut(s) | 1364, 1897, 1931, 2064, 2258, 2321, 2699, 3015 |
| BpuMI | CCSGG | 1 cut(s) | 2198 |
| Bsa29I | ATCGAT | 1 cut(s) | 873 |
| BsaAI | YACGTR | 1 cut(s) | 1086 |
| BsaBI | GATNNNNATC | 2 cut(s) | 177, 527 |
| BsaHI | GRCGYC | 2 cut(s) | 330, 779 |
| BsaI | GGTCTC | 2 cut(s) | 2026, 2620 |
| BsaJI | CCNNGG | 4 cut(s) | 435, 577, 1050, 1581 |
| BsaWI | WCCGGW | 2 cut(s) | 591, 2578 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2560, 2590 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 224, 241, 358, 436, 691, 717 |
| Bse118I | RCCGGY | 1 cut(s) | 717 |
| Bse1I | ACTGG | 5 cut(s) | 556, 1824, 2040, 2347, 2898 |
| Bse21I | CCTNAGG | 1 cut(s) | 1410 |
| Bse3DI | GCAATG | 3 cut(s) | 185, 2775, 3036 |
| Bse8I | GATNNNNATC | 2 cut(s) | 177, 527 |
| BseAI | TCCGGA | 1 cut(s) | 2578 |
| BseBI | CCWGG | 3 cut(s) | 263, 698, 1582 |
| BseCI | ATCGAT | 1 cut(s) | 873 |
| BseDI | CCNNGG | 4 cut(s) | 435, 577, 1050, 1581 |
| BseJI | GATNNNNATC | 2 cut(s) | 177, 527 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 224, 241, 358, 436, 691, 717 |
| BseMI | GCAATG | 3 cut(s) | 185, 2775, 3036 |
| BseMII | CTCAG | 8 cut(s) | 1219, 1343, 1401, 1692, 1751, 2061, 2769, 3058 |
| BseNI | ACTGG | 5 cut(s) | 556, 1824, 2040, 2347, 2898 |
| BsePI | GCGCGC | 2 cut(s) | 750, 834 |
| BseRI | GAGGAG | 6 cut(s) | 147, 362, 365, 449, 452, 455 |
| BseSI | GKGCMC | 2 cut(s) | 284, 1213 |
| BseXI | GCAGC | 9 cut(s) | 708, 1063, 1085, 1512, 1536, 1801, 2016, 2203, 2905 |
| BseYI | CCCAGC | 1 cut(s) | 727 |
| BsgI | GTGCAG | 3 cut(s) | 238, 1800, 1821 |
| Bsh1236I | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| BshFI | GGCC | 6 cut(s) | 65, 362, 1774, 2225, 2525, 3084 |
| BshNI | GGYRCC | 2 cut(s) | 281, 1054 |
| BshVI | ATCGAT | 1 cut(s) | 873 |
| BsiHKAI | GWGCWC | 4 cut(s) | 666, 1206, 1424, 1757 |
| BsiHKCI | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| BsiSI | CCGG | 8 cut(s) | 515, 592, 685, 718, 755, 1029, 2197, 2579 |
| BslFI | GGGAC | 3 cut(s) | 544, 1807, 3068 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 224, 241, 358, 436, 691, 717 |
| BsmAI | GTCTC | 5 cut(s) | 728, 1907, 2026, 2620, 2654 |
| BsmBI | CGTCTC | 1 cut(s) | 728 |
| BsmFI | GGGAC | 3 cut(s) | 544, 1807, 3068 |
| BsmI | GAATGC | 3 cut(s) | 694, 2242, 2388 |
| BsnI | GGCC | 6 cut(s) | 65, 362, 1774, 2225, 2525, 3084 |
| Bso31I | GGTCTC | 2 cut(s) | 2026, 2620 |
| BsoBI | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| Bsp1286I | GDGCHC | 6 cut(s) | 284, 666, 1206, 1213, 1424, 1757 |
| Bsp13I | TCCGGA | 1 cut(s) | 2578 |
| BspANI | GGCC | 6 cut(s) | 65, 362, 1774, 2225, 2525, 3084 |
| BspCNI | CTCAG | 8 cut(s) | 1218, 1342, 1402, 1693, 1750, 2060, 2770, 3057 |
| BspDI | ATCGAT | 1 cut(s) | 873 |
| BspEI | TCCGGA | 1 cut(s) | 2578 |
| BspFNI | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| BspLI | GGNNCC | 7 cut(s) | 201, 283, 596, 792, 1033, 1056, 1795 |
| BspT107I | GGYRCC | 2 cut(s) | 281, 1054 |
| BspTI | CTTAAG | 1 cut(s) | 1157 |
| BspTNI | GGTCTC | 2 cut(s) | 2026, 2620 |
| BsrDI | GCAATG | 3 cut(s) | 185, 2775, 3036 |
| BsrFI | RCCGGY | 1 cut(s) | 717 |
| BsrI | ACTGG | 5 cut(s) | 556, 1824, 2040, 2347, 2898 |
| BssAI | RCCGGY | 1 cut(s) | 717 |
| BssECI | CCNNGG | 4 cut(s) | 435, 577, 1050, 1581 |
| BssHII | GCGCGC | 2 cut(s) | 750, 834 |
| BssNAI | GTATAC | 1 cut(s) | 2656 |
| BssNI | GRCGYC | 2 cut(s) | 330, 779 |
| Bst1107I | GTATAC | 1 cut(s) | 2656 |
| Bst2UI | CCWGG | 3 cut(s) | 263, 698, 1582 |
| Bst6I | CTCTTC | 4 cut(s) | 407, 1953, 2623, 2930 |
| BstACI | GRCGYC | 2 cut(s) | 330, 779 |
| BstAFI | CTTAAG | 1 cut(s) | 1157 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1061, 1721, 2747 |
| BstBAI | YACGTR | 1 cut(s) | 1086 |
| BstC8I | GCNNGC | 8 cut(s) | 752, 836, 1154, 1380, 1420, 1927, 2181, 2297 |
| BstDSI | CCRYGG | 1 cut(s) | 577 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 356 |
| BstFNI | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| BstH2I | RGCGCY | 1 cut(s) | 1478 |
| BstHHI | GCGC | 7 cut(s) | 103, 684, 752, 754, 836, 838, 1477 |
| BstMAI | GTCTC | 5 cut(s) | 728, 1907, 2026, 2620, 2654 |
| BstNI | CCWGG | 3 cut(s) | 263, 698, 1582 |
| BstSCI | CCNGG | 4 cut(s) | 261, 696, 1580, 2196 |
| BstSFI | CTRYAG | 1 cut(s) | 937 |
| BstSLI | GKGCMC | 2 cut(s) | 284, 1213 |
| BstSNI | TACGTA | 1 cut(s) | 1086 |
| BstUI | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| BstV1I | GCAGC | 9 cut(s) | 708, 1063, 1085, 1512, 1536, 1801, 2016, 2203, 2905 |
| BstV2I | GAAGAC | 3 cut(s) | 1250, 1331, 1755 |
| BstX2I | RGATCY | 7 cut(s) | 152, 178, 199, 209, 227, 1569, 1853 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 14 |
| BstYI | RGATCY | 7 cut(s) | 152, 178, 199, 209, 227, 1569, 1853 |
| BstZ17I | GTATAC | 1 cut(s) | 2656 |
| Bsu15I | ATCGAT | 1 cut(s) | 873 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1410 |
| BsuI | GTATCC | 2 cut(s) | 1549, 2999 |
| BsuRI | GGCC | 6 cut(s) | 65, 362, 1774, 2225, 2525, 3084 |
| BsuTUI | ATCGAT | 1 cut(s) | 873 |
| BtgI | CCRYGG | 1 cut(s) | 577 |
| BtrI | CACGTC | 1 cut(s) | 1483 |
| BtsI | GCAGTG | 2 cut(s) | 1673, 1692 |
| BtsIMutI | CAGTG | 6 cut(s) | 1211, 1673, 1692, 1817, 2416, 2891 |
| Cac8I | GCNNGC | 8 cut(s) | 752, 836, 1154, 1380, 1420, 1927, 2181, 2297 |
| CfoI | GCGC | 7 cut(s) | 103, 684, 752, 754, 836, 838, 1477 |
| Cfr10I | RCCGGY | 1 cut(s) | 717 |
| Cfr42I | CCGCGG | 1 cut(s) | 580 |
| ClaI | ATCGAT | 1 cut(s) | 873 |
| CseI | GACGC | 2 cut(s) | 768, 2543 |
| Csp6I | GTAC | 4 cut(s) | 571, 715, 967, 1590 |
| CviAII | CATG | 9 cut(s) | 367, 706, 1595, 2017, 2154, 2290, 2745, 2846, 2866 |
| CviQI | GTAC | 4 cut(s) | 571, 715, 967, 1590 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 446 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 446 |
| EaeI | YGGCCR | 1 cut(s) | 2523 |
| Eam1104I | CTCTTC | 4 cut(s) | 407, 1953, 2623, 2930 |
| EarI | CTCTTC | 4 cut(s) | 407, 1953, 2623, 2930 |
| EciI | GGCGGA | 1 cut(s) | 192 |
| Eco105I | TACGTA | 1 cut(s) | 1086 |
| Eco147I | AGGCCT | 1 cut(s) | 1774 |
| Eco31I | GGTCTC | 2 cut(s) | 2026, 2620 |
| Eco47I | GGWCC | 7 cut(s) | 265, 439, 508, 694, 790, 1794, 2317 |
| Eco47III | AGCGCT | 1 cut(s) | 1476 |
| Eco57I | CTGAAG | 5 cut(s) | 374, 1455, 2378, 2448, 2486 |
| Eco81I | CCTNAGG | 1 cut(s) | 1410 |
| Eco88I | CYCGRG | 3 cut(s) | 473, 1750, 1894 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 356 |
| EcoO109I | RGGNCCY | 3 cut(s) | 508, 790, 1794 |
| EcoRII | CCWGG | 3 cut(s) | 261, 696, 1580 |
| Esp3I | CGTCTC | 1 cut(s) | 728 |
| FaeI | CATG | 9 cut(s) | 370, 709, 1598, 2020, 2157, 2293, 2748, 2849, 2869 |
| FaqI | GGGAC | 3 cut(s) | 544, 1807, 3068 |
| FatI | CATG | 9 cut(s) | 366, 705, 1594, 2016, 2153, 2289, 2744, 2845, 2865 |
| FauI | CCCGC | 2 cut(s) | 295, 1285 |
| FauNDI | CATATG | 1 cut(s) | 2434 |
| FbaI | TGATCA | 1 cut(s) | 2013 |
| FblI | GTMKAC | 2 cut(s) | 2158, 2655 |
| FspBI | CTAG | 4 cut(s) | 1005, 1767, 2147, 2573 |
| GlaI | GCGC | 7 cut(s) | 102, 683, 751, 753, 835, 837, 1476 |
| GsaI | CCCAGC | 1 cut(s) | 731 |
| GsuI | CTGGAG | 2 cut(s) | 539, 2364 |
| HaeII | RGCGCY | 1 cut(s) | 1478 |
| HaeIII | GGCC | 6 cut(s) | 65, 362, 1774, 2225, 2525, 3084 |
| HapII | CCGG | 8 cut(s) | 515, 592, 685, 718, 755, 1029, 2197, 2579 |
| HgaI | GACGC | 2 cut(s) | 768, 2543 |
| HhaI | GCGC | 7 cut(s) | 103, 684, 752, 754, 836, 838, 1477 |
| Hin1I | GRCGYC | 2 cut(s) | 330, 779 |
| Hin1II | CATG | 9 cut(s) | 370, 709, 1598, 2020, 2157, 2293, 2748, 2849, 2869 |
| Hin6I | GCGC | 7 cut(s) | 101, 682, 750, 752, 834, 836, 1475 |
| HinP1I | GCGC | 7 cut(s) | 101, 682, 750, 752, 834, 836, 1475 |
| HindIII | AAGCTT | 1 cut(s) | 1940 |
| HpaII | CCGG | 8 cut(s) | 515, 592, 685, 718, 755, 1029, 2197, 2579 |
| HphI | GGTGA | 7 cut(s) | 400, 965, 1625, 2135, 2546, 2974, 3055 |
| Hpy166II | GTNNAC | 8 cut(s) | 115, 268, 715, 1590, 1987, 2159, 2352, 2656 |
| Hpy188III | TCNNGA | 9 cut(s) | 451, 475, 1250, 1409, 2081, 2275, 2465, 2579, 2822 |
| Hpy8I | GTNNAC | 8 cut(s) | 115, 268, 715, 1590, 1987, 2159, 2352, 2656 |
| Hpy99I | CGWCG | 4 cut(s) | 332, 335, 784, 799 |
| HpyCH4IV | ACGT | 6 cut(s) | 330, 423, 737, 1085, 1482, 2335 |
| HpySE526I | ACGT | 6 cut(s) | 330, 423, 737, 1085, 1482, 2335 |
| Hsp92I | GRCGYC | 2 cut(s) | 330, 779 |
| Hsp92II | CATG | 9 cut(s) | 370, 709, 1598, 2020, 2157, 2293, 2748, 2849, 2869 |
| HspAI | GCGC | 7 cut(s) | 101, 682, 750, 752, 834, 836, 1475 |
| Kpn2I | TCCGGA | 1 cut(s) | 2578 |
| Ksp22I | TGATCA | 1 cut(s) | 2013 |
| KspI | CCGCGG | 1 cut(s) | 580 |
| LmnI | GCTCC | 8 cut(s) | 375, 462, 594, 661, 784, 1678, 2199, 2581 |
| Lsp1109I | GCAGC | 9 cut(s) | 708, 1063, 1085, 1512, 1536, 1801, 2016, 2203, 2905 |
| LweI | GCATC | 6 cut(s) | 1441, 1711, 2109, 2359, 2750, 2944 |
| MaeI | CTAG | 4 cut(s) | 1005, 1767, 2147, 2573 |
| MaeII | ACGT | 6 cut(s) | 330, 423, 737, 1085, 1482, 2335 |
| MaeIII | GTNAC | 4 cut(s) | 583, 900, 2437, 2470 |
| MflI | RGATCY | 7 cut(s) | 152, 178, 199, 209, 227, 1569, 1853 |
| MhlI | GDGCHC | 6 cut(s) | 284, 666, 1206, 1213, 1424, 1757 |
| MlsI | TGGCCA | 1 cut(s) | 2525 |
| MluI | ACGCGT | 1 cut(s) | 1389 |
| MluNI | TGGCCA | 1 cut(s) | 2525 |
| MmeI | TCCRAC | 6 cut(s) | 31, 96, 206, 762, 897, 2286 |
| Mox20I | TGGCCA | 1 cut(s) | 2525 |
| MroI | TCCGGA | 1 cut(s) | 2578 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1962, 2499 |
| MscI | TGGCCA | 1 cut(s) | 2525 |
| MslI | CAYNNNNRTG | 3 cut(s) | 710, 1427, 2121 |
| Msp20I | TGGCCA | 1 cut(s) | 2525 |
| MspA1I | CMGCKG | 4 cut(s) | 579, 634, 727, 1076 |
| MspCI | CTTAAG | 1 cut(s) | 1157 |
| MspI | CCGG | 8 cut(s) | 515, 592, 685, 718, 755, 1029, 2197, 2579 |
| MspR9I | CCNGG | 4 cut(s) | 263, 698, 1582, 2198 |
| Mva1269I | GAATGC | 3 cut(s) | 694, 2242, 2388 |
| MvaI | CCWGG | 3 cut(s) | 263, 698, 1582 |
| MvnI | CGCG | 6 cut(s) | 101, 579, 752, 836, 1280, 1391 |
| NciI | CCSGG | 1 cut(s) | 2198 |
| NdeI | CATATG | 1 cut(s) | 2434 |
| NlaIII | CATG | 9 cut(s) | 370, 709, 1598, 2020, 2157, 2293, 2748, 2849, 2869 |
| NlaIV | GGNNCC | 7 cut(s) | 201, 283, 596, 792, 1033, 1056, 1795 |
| NmeAIII | GCCGAG | 2 cut(s) | 231, 1031 |
| NmuCI | GTSAC | 1 cut(s) | 583 |
| OliI | CACNNNNGTG | 1 cut(s) | 1427 |
| PaeR7I | CTCGAG | 2 cut(s) | 473, 1750 |
| PauI | GCGCGC | 2 cut(s) | 750, 834 |
| PceI | AGGCCT | 1 cut(s) | 1774 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 203, 324, 480, 489 |
| PctI | GAATGC | 3 cut(s) | 694, 2242, 2388 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1962, 2499 |
| PfeI | GAWTC | 8 cut(s) | 173, 523, 646, 1457, 1963, 2406, 2582, 3015 |
| PfoI | TCCNGGA | 1 cut(s) | 2196 |
| Ppu21I | YACGTR | 1 cut(s) | 1086 |
| PpuMI | RGGWCCY | 3 cut(s) | 508, 790, 1794 |
| PsiI | TTATAA | 1 cut(s) | 980 |
| Psp5II | RGGWCCY | 3 cut(s) | 508, 790, 1794 |
| Psp6I | CCWGG | 3 cut(s) | 261, 696, 1580 |
| PspFI | CCCAGC | 1 cut(s) | 727 |
| PspGI | CCWGG | 3 cut(s) | 261, 696, 1580 |
| PspN4I | GGNNCC | 7 cut(s) | 201, 283, 596, 792, 1033, 1056, 1795 |
| PspPPI | RGGWCCY | 3 cut(s) | 508, 790, 1794 |
| PspXI | VCTCGAGB | 1 cut(s) | 1750 |
| PsuI | RGATCY | 7 cut(s) | 152, 178, 199, 209, 227, 1569, 1853 |
| PteI | GCGCGC | 2 cut(s) | 750, 834 |
| PvuII | CAGCTG | 1 cut(s) | 1076 |
| RsaI | GTAC | 4 cut(s) | 572, 716, 968, 1591 |
| RsaNI | GTAC | 4 cut(s) | 571, 715, 967, 1590 |
| RseI | CAYNNNNRTG | 3 cut(s) | 710, 1427, 2121 |
| SacII | CCGCGG | 1 cut(s) | 580 |
| ScrFI | CCNGG | 4 cut(s) | 263, 698, 1582, 2198 |
| SduI | GDGCHC | 6 cut(s) | 284, 666, 1206, 1213, 1424, 1757 |
| SfaNI | GCATC | 6 cut(s) | 1441, 1711, 2109, 2359, 2750, 2944 |
| SfcI | CTRYAG | 1 cut(s) | 937 |
| Sfr274I | CTCGAG | 2 cut(s) | 473, 1750 |
| Sfr303I | CCGCGG | 1 cut(s) | 580 |
| SgrBI | CCGCGG | 1 cut(s) | 580 |
| SinI | GGWCC | 7 cut(s) | 265, 439, 508, 694, 790, 1794, 2317 |
| SlaI | CTCGAG | 2 cut(s) | 473, 1750 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 710, 1427, 2121 |
| SnaBI | TACGTA | 1 cut(s) | 1086 |
| SseBI | AGGCCT | 1 cut(s) | 1774 |
| SspMI | CTAG | 4 cut(s) | 1005, 1767, 2147, 2573 |
| StuI | AGGCCT | 1 cut(s) | 1774 |
| StyD4I | CCNGG | 4 cut(s) | 261, 696, 1580, 2196 |
| TaiI | ACGT | 6 cut(s) | 333, 426, 740, 1088, 1485, 2338 |
| TaqII | GACCGA | 1 cut(s) | 506 |
| TatI | WGTACW | 3 cut(s) | 570, 966, 1589 |
| TauI | GCSGC | 3 cut(s) | 634, 727, 3084 |
| TfiI | GAWTC | 8 cut(s) | 173, 523, 646, 1457, 1963, 2406, 2582, 3015 |
| TscAI | CASTG | 6 cut(s) | 1218, 1673, 1699, 1824, 2416, 2898 |
| TseFI | GTSAC | 1 cut(s) | 583 |
| TseI | GCWGC | 9 cut(s) | 721, 1073, 1076, 1525, 1549, 1814, 2029, 2191, 2918 |
| Tsp45I | GTSAC | 1 cut(s) | 583 |
| TspDTI | ATGAA | 2 cut(s) | 394, 2882 |
| TspGWI | ACGGA | 1 cut(s) | 700 |
| TspRI | CASTG | 6 cut(s) | 1218, 1673, 1699, 1824, 2416, 2898 |
| Vha464I | CTTAAG | 1 cut(s) | 1157 |
| VpaK11BI | GGWCC | 7 cut(s) | 265, 439, 508, 694, 790, 1794, 2317 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 356 |
| XapI | RAATTY | 2 cut(s) | 2506, 2834 |
| XhoI | CTCGAG | 2 cut(s) | 473, 1750 |
| XmiI | GTMKAC | 2 cut(s) | 2158, 2655 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1962, 2499 |
| XspI | CTAG | 4 cut(s) | 1005, 1767, 2147, 2573 |
| ZraI | GACGTC | 1 cut(s) | 331 |