Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000969

Physical Location & Seq

Genomic Coordinates Forward (+)

67792602 .. 67798016

Gene Structure 5415 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ51936

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGGAGAAGTCATGCACGCTGCTGGTCCACTTCGACAAGGGCACGCCGGCCCTGGCCAACGAGATCAAGGAAGCTCTGGAAGGAAACGACGTGGAAGCCAAGATCGAAGCCATGAAGAAGGCAATCATGATCTTGCTCAACGGCGACACAATTCCCCAGCTCTTCATCACCATTATCCGCTACGTCCTCCCCTCCGAGGACCACACCGTCCAGAAGCTTCTCCTCCTCTACCTCGAGATCATTGACAAGACAGATTCCCGAGGCAAGGTTCTCCCGGAGATGATCCTCATCTGCCAGAACCTCCGCAACAATCTCCAGAGCCCTAACGAGTACATCCGCGGCGTCACGCTCCGATTCCTCTGCCGGTTGAACGAGGCCGAGATCGTCGAGCCGTTGATTCCGTCGATTCTGGCGAATCTGGAGCACCGCCACCCGTTCGTTCGACGGAACGCCGTCAGCGCAGTCATGTCTGTGTATAGGCTTCCGCAGGGTGAGCAGTTGCTGGTGGATGCGCCGGAGATTATCGATCGGTTCTTGGCCTCCGAGCAAGACCCGTCCAGTAAGAGAAACGCTTTCCTTATGCTCTTTAATTGCGCGCAAGATAGAGCTGTTAGTTATCTGTTTACTCATGTCGATAGGATTATTGATTGGGCTGAGCAGCTCCAGATGGTTGTGTTGGAATTGATTAGGAAGGTTTGTAGAGTCAATAAGGGTGAGAAGGGGAAGTACATTAAGGTCATTATATCATTGCTTAATGCTCAGTCCAGTGCTGTTGTTTACGAGTGTGCCAATACGCTCGTGTCCTTGTCGTCTGCCCCCACTGCAATTCGAGCTGCAGCCAATACTTACTGTCAGTTGTTGTTGTCGCAGAGCGACAACAATGTGAAGTTGATTGTGCTTGATAGGATTAATGAGCTTAAGGCTTCGCATAGGGAGATTTTGGTTGAGTTGGTTATGGATGTGCTCAGGGCCGTGTCGAGTCCAAATCTTGATATTAGAAGGAAGACTCTTGATATTGTGCTTGACTTGATTACTCCTGGGAATATTGATGAGGTTGTGATGATGTTGAAGAAGGAGGTCGTCAAGACTCAGGGTGCTGAGCTTGAGAAGAACGGGGAATACAGGCAGATGCTGGTGCAAGCAATATATTCATGTGCAGTTAAGTTTCCTGCGGTGGCGAGCACGGTGGTCCATCCATTGATGGATTTCTTGGGGGATACCAATCTGGCCTCAGCTATGGATGTGGCTATTTTTGTGCGTGAGATTATTGAAACCAACCCCAAGTTGCGTGTCTCTATAATTACAAGGCTGTTGGATACTTTCTACCAGATACGGACTTCAAGGGTGTGTACTTATGCTCTTTGGATTATTGGGGAGTACTGTCTCTCACTTTCTGAGGTTGAGAGTGGGATTGCAACCATTAAGCAGTGTCTAGGGGAGCTCCCATTTTACACCGCTTCTGAGGAAAGCGAGGCACAAGATGTTTCGAAGAATTCTCAGGTGGTAAACTCATCTACTGTGTCTTCCAGGAGACCTGTAGTGCTTGCAGATGGGACCTATGCTACCCAGTCTGCTGCACTGGAAACTGCTATGTCTGCACCTGTCCTTGTTCAAGGATCTTTGGCTTCTGTAGGCAACTTGAGATCGTTGATTCTCTCAGGTGACTTTTTCCTGGAGTCTGTTGTGGCTTGCACTTTAACAAAGCTTGTTTTGAGACTAGATGAGGTCCAGCCTTCTAAAGTTGAAGTTAACAAGGCAACCACACAGGCTTTGTTGATCATGGTGTCCATGCTGCAACTGGGTCAATCATCAGTCCTTCCACATCCAATTGATAATGATTCTCAAGACAGAATTGTTCTTTGCATCCGTACACTATGCAATAAAGATGAAGAAGTGAAGAAGATATGGTTAAAGTCTTGTAGAGAGAGTTGTGTTAAAATGCTTGAAGATAAGAAGCTTCGGGAAATAGAGGAAAGTAGAGCTATGGCGCAGATATCAAATGCACAACCAGATGACCTTATTGATTTCTACCATCTTAAGAGCAGAAAGGGTATGAGCCAGCTTGAGTTGGAAGATGAGGTTCAAGATGATCTCAAACGTGCTACTGGAGAGTTTGTAAAGGATGGAGATGATGCAAATAAACTCAACCGCATTCTTCAGCTCACAGGATTCAGCGATCCTGTTTATGCTGAAGCTTATGTTACAAATTTATTCTTGGAGTTGGCCACGATGGGCGATCTCAAACTTGTTGAGCGTCCTCAGAACTATACACTAGCTCCAGAATCAACCAAACAGATAAAGGCAAACATTAAGGTCTCCTCAACTGAAACAGGAGTTATATTTGGTAACATTGTTTATGAGACGTCAAATGTGCTTGAGAGAACAGTTATTGTTCTTAATAACATCCATATTGATATAATGGATTACATCTCTCCTTCTTCATGTGCTGATGTGTTGTTTCGGACAATGTGGGCTGAGTTTGAATGGGAAAATAAGGTTGCTATAAGTACTGTTATCAAAGACGAGAAGGACTTTCTGAACCACATTGTTAAAGCAACTAACATGAAGTGCCTGACTCCACCATCGGCACTGGAAGGAGAGTGTGGAGTCCTCGCTGCCAATCTGTATGCAAAGAGTGTTTTCGGGGAGGATGCTTTGGTGAATGTGAGTGTTGAGAAACAAGCAGATGGTAAGCTAAGTGGATACATCAGAATTAGAAGCAAGACTCAAGGTATTGCACTGAGTCTTGGGGACAAGATCACCTTGAAACAGAAGGGTGGCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0005911 GO:0006810 GO:0006888 GO:0006890 GO:0006891 GO:0008150 GO:0009506 GO:0012505 GO:0012506 GO:0016020 GO:0016192 GO:0030054 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Adaptin_N Coatamer_beta_C Coatomer_b_Cpla

Protein Analysis

928

Amino Acids

103.41

Weight (kDa)

5.43

Isoelectric Point (pI)

44.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adaptin_N	PF01602	20 - 524	3e-92	Adaptin N terminal region
Coatamer_beta_C	PF07718	670 - 736	1.1e-13	Coatomer beta C-terminal region
Coatamer_beta_C	PF07718	735 - 787	1.5e-20	Coatomer beta C-terminal region
Coatomer_b_Cpla	PF14806	793 - 920	2.4e-55	Coatomer beta subunit appendage platform

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31480 AT4G31490 AT4G31490 AT4G31490
fragaria_vesca	FvH4_4g12700 FvH4_4g12700 FvH4_4g12700 FvH4_6g37230 FvH4_6g37230 FvH4_6g37230
malus_domestica	MD04G1023700.v1.1 MD09G1159400.v1.1 MD13G1280100.v1.1 MD17G1145600.v1.1
prunus_persica	Prupe.3G018300_v2.0.a1 Prupe.3G018400_v2.0.a1
pyrus_communis	pycom04g02030 pycom09g07780 pycom09g18330 pycom13g29300 pycom15g32330 pycom15g32340 pycom17g13850
rosa_chinensis	RchiOBHm_Chr2g0149941 RchiOBHm_Chr2g0149961 RchiOBHm_Chr2g0149991 RchiOBHm_Chr4g0411591
rosa_laevigata	RLG00000008343 RLG00000020457 RLG00000020458
rosa_multiflora	Rmu_sc0001899.1_g000002 Rmu_sc0006550.1_g000008
rosa_roxburghii	Rroxscaffold_2G00097530 Rroxscaffold_2G00097540 Rroxscaffold_5G00356170
rosa_rugosa	Rorug02G0417300 Rorug04G0107800
rosa_samantha	Rh2AG476800 Rh2AG476900 Rh2AG477100 Rh2BG488500 Rh2BG488600 Rh2CG462500 Rh2CG462600 Rh2CG462800 Rh2DG497600 Rh2DG497700 Rh4AG169200 Rh4BG166100 Rh4CG180900 Rh4DG163500
rosa_wichuraiana	Rw2G038870 Rw2G038880 Rw2G038900 Rw4G014060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	206
AatII	GACGTC	1 cut(s)	2366
AccII	CGCG	2 cut(s)	339, 596
AciI	CCGC	9 cut(s)	178, 304, 337, 339, 427, 485, 1172, 1455, 2149
AclWI	GGATC	3 cut(s)	277, 1624, 2171
AcoI	YGGCCR	2 cut(s)	54, 2223
AcsI	RAATTY	2 cut(s)	1492, 2206
AcuI	CTGAAG	2 cut(s)	2141, 2211
AcyI	GRCGYC	2 cut(s)	342, 2363
AfaI	GTAC	6 cut(s)	332, 728, 1351, 1379, 1870, 2509
AfiI	CCNNNNNNNGG	2 cut(s)	196, 265
AflII	CTTAAG	2 cut(s)	917, 2036
AjiI	CACGTC	1 cut(s)	91
AjnI	CCWGG	4 cut(s)	51, 1036, 1526, 1671
Alw21I	GWGCWC	4 cut(s)	426, 966, 1184, 1443
Alw26I	GTCTC	6 cut(s)	1297, 1388, 1525, 1708, 2320, 2354
AlwI	GGATC	3 cut(s)	277, 1624, 2171
AlwNI	CAGNNNCTG	2 cut(s)	502, 1132
Ama87I	CYCGRG	2 cut(s)	233, 258
AoxI	GGCC	8 cut(s)	48, 54, 375, 537, 969, 1227, 2223, 2779
ApeKI	GCWGC	7 cut(s)	19, 658, 833, 836, 1574, 1792, 2615
ApoI	RAATTY	2 cut(s)	1492, 2206
AseI	ATTAAT	1 cut(s)	909
AspLEI	GCGC	5 cut(s)	461, 514, 596, 598, 1990
AspS9I	GGNCC	8 cut(s)	25, 49, 199, 969, 1189, 1554, 1726, 2780
AsuC2I	CCSGG	1 cut(s)	275
AsuHPI	GGTGA	6 cut(s)	160, 503, 725, 1673, 2671, 2752
AsuII	TTCGAA	1 cut(s)	1487
AvaI	CYCGRG	2 cut(s)	233, 258
AvaII	GGWCC	5 cut(s)	25, 199, 1189, 1554, 1726
BaeGI	GKGCMC	1 cut(s)	44
BalI	TGGCCA	2 cut(s)	56, 2225
BanII	GRGCYC	2 cut(s)	323, 1443
BarI	GAAGNNNNNNTAC	2 cut(s)	710, 742
BauI	CACGAG	1 cut(s)	797
BbsI	GAAGAC	2 cut(s)	1010, 1515
Bbv12I	GWGCWC	4 cut(s)	426, 966, 1184, 1443
BbvCI	CCTCAGC	1 cut(s)	1231
BbvI	GCAGC	7 cut(s)	6, 670, 820, 848, 1561, 1779, 2602
BccI	CCATC	9 cut(s)	661, 1195, 1200, 1544, 2040, 2117, 2224, 2590, 2681
BceAI	ACGGC	4 cut(s)	157, 376, 437, 956
BcgI	CGANNNNNNTGC	2 cut(s)	342, 376
BciT130I	CCWGG	4 cut(s)	53, 1038, 1528, 1673
BciVI	GTATCC	3 cut(s)	1210, 1309, 2696
BclI	TGATCA	1 cut(s)	1776
BcnI	CCSGG	1 cut(s)	275
BcoDI	GTCTC	6 cut(s)	1297, 1388, 1525, 1708, 2320, 2354
BfaI	CTAG	3 cut(s)	1433, 1718, 2273
BfmI	CTRYAG	3 cut(s)	834, 1536, 1629
BfrI	CTTAAG	2 cut(s)	917, 2036
BfuI	GTATCC	3 cut(s)	1210, 1309, 2696
BisI	GCNGC	8 cut(s)	20, 340, 659, 834, 837, 1575, 1793, 2616
BlpI	GCTNAGC	2 cut(s)	654, 1098
BlsI	GCNGC	8 cut(s)	21, 341, 660, 835, 838, 1576, 1794, 2617
BmcAI	AGTACT	2 cut(s)	1379, 2509
Bme1390I	CCNGG	5 cut(s)	53, 275, 1038, 1528, 1673
Bme18I	GGWCC	5 cut(s)	25, 199, 1189, 1554, 1726
BmeT110I	CYCGRG	2 cut(s)	233, 258
BmgBI	CACGTC	1 cut(s)	91
BmgT120I	GGNCC	8 cut(s)	25, 49, 199, 969, 1189, 1554, 1726, 2780
BmiI	GGNNCC	1 cut(s)	1555
BmrFI	CCNGG	5 cut(s)	53, 275, 1038, 1528, 1673
BmrI	ACTGGG	2 cut(s)	1561, 1808
BmsI	GCATC	5 cut(s)	499, 1119, 1872, 2122, 2641
BmuI	ACTGGG	2 cut(s)	1561, 1808
BpiI	GAAGAC	2 cut(s)	1010, 1515
BpmI	CTGGAG	6 cut(s)	299, 440, 647, 1694, 2127, 2262
Bpu10I	CCTNAGC	2 cut(s)	965, 1231
Bpu1102I	GCTNAGC	2 cut(s)	654, 1098
Bpu14I	TTCGAA	1 cut(s)	1487
BpuEI	CTTGAG	6 cut(s)	1124, 1660, 1827, 2084, 2396, 2712
BpuMI	CCSGG	1 cut(s)	275
Bsa29I	ATCGAT	1 cut(s)	525
BsaBI	GATNNNNATC	2 cut(s)	287, 1221
BsaHI	GRCGYC	2 cut(s)	342, 2363
BsaI	GGTCTC	2 cut(s)	1525, 2320
BsaJI	CCNNGG	5 cut(s)	51, 195, 259, 337, 1037
BsaXI	ACNNNNNCTCC	4 cut(s)	2100, 2130, 2260, 2290
Bsc4I	CCNNNNNNNGG	2 cut(s)	196, 265
Bse118I	RCCGGY	2 cut(s)	46, 363
Bse1I	ACTGG	7 cut(s)	558, 765, 1567, 1584, 1803, 2110, 2595
Bse3DI	GCAATG	1 cut(s)	746
Bse8I	GATNNNNATC	2 cut(s)	287, 1221
BseBI	CCWGG	4 cut(s)	53, 1038, 1528, 1673
BseCI	ATCGAT	1 cut(s)	525
BseDI	CCNNGG	5 cut(s)	51, 195, 259, 337, 1037
BseJI	GATNNNNATC	2 cut(s)	287, 1221
BseLI	CCNNNNNNNGG	2 cut(s)	196, 265
BseMI	GCAATG	1 cut(s)	746
BseNI	ACTGG	7 cut(s)	558, 765, 1567, 1584, 1803, 2110, 2595
BsePI	GCGCGC	1 cut(s)	594
BseRI	GAGGAG	3 cut(s)	212, 215, 2308
BseSI	GKGCMC	1 cut(s)	44
BseXI	GCAGC	7 cut(s)	6, 670, 820, 848, 1561, 1779, 2602
BseYI	CCCAGC	1 cut(s)	156
BsgI	GTGCAG	3 cut(s)	1176, 1560, 1581
Bsh1236I	CGCG	2 cut(s)	339, 596
Bsh1285I	CGRYCG	1 cut(s)	529
BshFI	GGCC	8 cut(s)	50, 56, 377, 539, 971, 1229, 2225, 2781
BshVI	ATCGAT	1 cut(s)	525
BsiEI	CGRYCG	1 cut(s)	529
BsiHKAI	GWGCWC	4 cut(s)	426, 966, 1184, 1443
BsiHKCI	CYCGRG	2 cut(s)	233, 258
BsiSI	CCGG	4 cut(s)	47, 275, 364, 515
BslFI	GGGAC	2 cut(s)	1567, 2765
BslI	CCNNNNNNNGG	2 cut(s)	196, 265
BsmAI	GTCTC	6 cut(s)	1297, 1388, 1525, 1708, 2320, 2354
BsmBI	CGTCTC	1 cut(s)	2354
BsmFI	GGGAC	2 cut(s)	1567, 2765
BsmI	GAATGC	1 cut(s)	2151
BsnI	GGCC	8 cut(s)	50, 56, 377, 539, 971, 1229, 2225, 2781
Bso31I	GGTCTC	2 cut(s)	1525, 2320
BsoBI	CYCGRG	2 cut(s)	233, 258
Bsp119I	TTCGAA	1 cut(s)	1487
Bsp1286I	GDGCHC	6 cut(s)	44, 323, 426, 966, 1184, 1443
Bsp1720I	GCTNAGC	2 cut(s)	654, 1098
BspACI	CCGC	9 cut(s)	178, 304, 337, 339, 427, 485, 1172, 1455, 2149
BspANI	GGCC	8 cut(s)	50, 56, 377, 539, 971, 1229, 2225, 2781
BspDI	ATCGAT	1 cut(s)	525
BspFNI	CGCG	2 cut(s)	339, 596
BspHI	TCATGA	1 cut(s)	126
BspLI	GGNNCC	1 cut(s)	1555
BspMAI	CTGCAG	1 cut(s)	838
BspPI	GGATC	3 cut(s)	277, 1624, 2171
BspQI	GCTCTTC	1 cut(s)	167
BspT104I	TTCGAA	1 cut(s)	1487
BspTI	CTTAAG	2 cut(s)	917, 2036
BspTNI	GGTCTC	2 cut(s)	1525, 2320
BsrDI	GCAATG	1 cut(s)	746
BsrFI	RCCGGY	2 cut(s)	46, 363
BsrI	ACTGG	7 cut(s)	558, 765, 1567, 1584, 1803, 2110, 2595
BssAI	RCCGGY	2 cut(s)	46, 363
BssECI	CCNNGG	5 cut(s)	51, 195, 259, 337, 1037
BssHII	GCGCGC	1 cut(s)	594
BssNI	GRCGYC	2 cut(s)	342, 2363
BssSI	CACGAG	1 cut(s)	797
Bst2BI	CACGAG	1 cut(s)	797
Bst2UI	CCWGG	4 cut(s)	53, 1038, 1528, 1673
Bst4CI	ACNGT	7 cut(s)	208, 851, 1186, 1382, 1519, 2386, 2512
Bst6I	CTCTTC	1 cut(s)	167
BstACI	GRCGYC	2 cut(s)	342, 2363
BstAFI	CTTAAG	2 cut(s)	917, 2036
BstBI	TTCGAA	1 cut(s)	1487
BstC8I	GCNNGC	9 cut(s)	17, 44, 48, 596, 1140, 1180, 1545, 1690, 2060
BstDSI	CCRYGG	1 cut(s)	337
BstFNI	CGCG	2 cut(s)	339, 596
BstHHI	GCGC	5 cut(s)	461, 514, 596, 598, 1990
BstMAI	GTCTC	6 cut(s)	1297, 1388, 1525, 1708, 2320, 2354
BstMCI	CGRYCG	1 cut(s)	529
BstMWI	GCNNNNNNNGC	5 cut(s)	458, 493, 821, 830, 1595
BstNI	CCWGG	4 cut(s)	53, 1038, 1528, 1673
BstSCI	CCNGG	5 cut(s)	51, 273, 1036, 1526, 1671
BstSFI	CTRYAG	3 cut(s)	834, 1536, 1629
BstSLI	GKGCMC	1 cut(s)	44
BstUI	CGCG	2 cut(s)	339, 596
BstV1I	GCAGC	7 cut(s)	6, 670, 820, 848, 1561, 1779, 2602
BstV2I	GAAGAC	2 cut(s)	1010, 1515
BstX2I	RGATCY	1 cut(s)	1616
BstYI	RGATCY	1 cut(s)	1616
Bsu15I	ATCGAT	1 cut(s)	525
BsuI	GTATCC	3 cut(s)	1210, 1309, 2696
BsuRI	GGCC	8 cut(s)	50, 56, 377, 539, 971, 1229, 2225, 2781
BsuTUI	ATCGAT	1 cut(s)	525
BtgI	CCRYGG	1 cut(s)	337
BtrI	CACGTC	1 cut(s)	91
BtsI	GCAGTG	2 cut(s)	819, 1433
BtsIMutI	CAGTG	6 cut(s)	772, 819, 1433, 1577, 2588, 2738
Cac8I	GCNNGC	9 cut(s)	17, 44, 48, 596, 1140, 1180, 1545, 1690, 2060
CaiI	CAGNNNCTG	2 cut(s)	502, 1132
CciI	TCATGA	1 cut(s)	126
CfoI	GCGC	5 cut(s)	461, 514, 596, 598, 1990
Cfr10I	RCCGGY	2 cut(s)	46, 363
Cfr13I	GGNCC	8 cut(s)	25, 49, 199, 969, 1189, 1554, 1726, 2780
Cfr42I	CCGCGG	1 cut(s)	340
ClaI	ATCGAT	1 cut(s)	525
CseI	GACGC	2 cut(s)	331, 2243
Csp6I	GTAC	6 cut(s)	331, 727, 1350, 1378, 1869, 2508
CviQI	GTAC	6 cut(s)	331, 727, 1350, 1378, 1869, 2508
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
EaeI	YGGCCR	2 cut(s)	54, 2223
Eam1104I	CTCTTC	1 cut(s)	167
EarI	CTCTTC	1 cut(s)	167
Ecl136II	GAGCTC	1 cut(s)	1441
Eco24I	GRGCYC	2 cut(s)	323, 1443
Eco31I	GGTCTC	2 cut(s)	1525, 2320
Eco32I	GATATC	1 cut(s)	1995
Eco47I	GGWCC	5 cut(s)	25, 199, 1189, 1554, 1726
Eco53kI	GAGCTC	1 cut(s)	1441
Eco57I	CTGAAG	2 cut(s)	2141, 2211
Eco88I	CYCGRG	2 cut(s)	233, 258
EcoICRI	GAGCTC	1 cut(s)	1441
EcoO109I	RGGNCCY	1 cut(s)	1554
EcoRI	GAATTC	1 cut(s)	1492
EcoRII	CCWGG	4 cut(s)	51, 1036, 1526, 1671
EcoRV	GATATC	1 cut(s)	1995
EcoT38I	GRGCYC	2 cut(s)	323, 1443
Esp3I	CGTCTC	1 cut(s)	2354
FalI	AAGNNNNNCTT	2 cut(s)	2747, 2779
FaqI	GGGAC	2 cut(s)	1567, 2765
FbaI	TGATCA	1 cut(s)	1776
Fnu4HI	GCNGC	8 cut(s)	20, 340, 659, 834, 837, 1575, 1793, 2616
FriOI	GRGCYC	2 cut(s)	323, 1443
Fsp4HI	GCNGC	8 cut(s)	20, 340, 659, 834, 837, 1575, 1793, 2616
FspBI	CTAG	3 cut(s)	1433, 1718, 2273
GlaI	GCGC	5 cut(s)	460, 513, 595, 597, 1989
GluI	GCNGC	8 cut(s)	20, 340, 659, 834, 837, 1575, 1793, 2616
GsaI	CCCAGC	1 cut(s)	160
GsuI	CTGGAG	6 cut(s)	299, 440, 647, 1694, 2127, 2262
HaeIII	GGCC	8 cut(s)	50, 56, 377, 539, 971, 1229, 2225, 2781
HapII	CCGG	4 cut(s)	47, 275, 364, 515
HgaI	GACGC	2 cut(s)	331, 2243
HhaI	GCGC	5 cut(s)	461, 514, 596, 598, 1990
Hin1I	GRCGYC	2 cut(s)	342, 2363
Hin6I	GCGC	5 cut(s)	459, 512, 594, 596, 1988
HinP1I	GCGC	5 cut(s)	459, 512, 594, 596, 1988
HincII	GTYRAC	1 cut(s)	1750
HindII	GTYRAC	1 cut(s)	1750
HindIII	AAGCTT	4 cut(s)	215, 1703, 1955, 2193
HpaI	GTTAAC	1 cut(s)	1750
HpaII	CCGG	4 cut(s)	47, 275, 364, 515
HphI	GGTGA	6 cut(s)	160, 503, 725, 1673, 2671, 2752
Hpy166II	GTNNAC	7 cut(s)	28, 624, 778, 1350, 1507, 1750, 1871
Hpy188I	TCNGA	9 cut(s)	196, 353, 544, 1396, 1462, 2262, 2463, 2538, 2711
Hpy8I	GTNNAC	7 cut(s)	28, 624, 778, 1350, 1507, 1750, 1871
Hpy99I	CGWCG	4 cut(s)	92, 389, 406, 447
HpyCH4III	ACNGT	7 cut(s)	208, 851, 1186, 1382, 1519, 2386, 2512
HpyCH4IV	ACGT	4 cut(s)	90, 183, 2098, 2363
HpyF10VI	GCNNNNNNNGC	5 cut(s)	458, 493, 821, 830, 1595
HpySE526I	ACGT	4 cut(s)	90, 183, 2098, 2363
Hsp92I	GRCGYC	2 cut(s)	342, 2363
HspAI	GCGC	5 cut(s)	459, 512, 594, 596, 1988
KroI	GCCGGC	1 cut(s)	46
KroNI	GCCGGC	1 cut(s)	48
Ksp22I	TGATCA	1 cut(s)	1776
KspAI	GTTAAC	1 cut(s)	1750
KspI	CCGCGG	1 cut(s)	340
LguI	GCTCTTC	1 cut(s)	167
LmnI	GCTCC	6 cut(s)	354, 421, 666, 1438, 1446, 2281
Lsp1109I	GCAGC	7 cut(s)	6, 670, 820, 848, 1561, 1779, 2602
LweI	GCATC	5 cut(s)	499, 1119, 1872, 2122, 2641
MaeI	CTAG	3 cut(s)	1433, 1718, 2273
MaeII	ACGT	4 cut(s)	90, 183, 2098, 2363
MaeIII	GTNAC	4 cut(s)	343, 1661, 2200, 2345
MfeI	CAATTG	1 cut(s)	1827
MflI	RGATCY	1 cut(s)	1616
MhlI	GDGCHC	6 cut(s)	44, 323, 426, 966, 1184, 1443
MlsI	TGGCCA	2 cut(s)	56, 2225
MluNI	TGGCCA	2 cut(s)	56, 2225
MlyI	GAGTC	9 cut(s)	711, 988, 1000, 1081, 1685, 2569, 2616, 2719, 2752
MmeI	TCCRAC	3 cut(s)	657, 1293, 2049
Mox20I	TGGCCA	2 cut(s)	56, 2225
MroNI	GCCGGC	1 cut(s)	46
MscI	TGGCCA	2 cut(s)	56, 2225
MslI	CAYNNNNRTG	1 cut(s)	470
Msp20I	TGGCCA	2 cut(s)	56, 2225
MspA1I	CMGCKG	1 cut(s)	339
MspCI	CTTAAG	2 cut(s)	917, 2036
MspI	CCGG	4 cut(s)	47, 275, 364, 515
MspR9I	CCNGG	5 cut(s)	53, 275, 1038, 1528, 1673
MunI	CAATTG	1 cut(s)	1827
Mva1269I	GAATGC	1 cut(s)	2151
MvaI	CCWGG	4 cut(s)	53, 1038, 1528, 1673
MvnI	CGCG	2 cut(s)	339, 596
MwoI	GCNNNNNNNGC	5 cut(s)	458, 493, 821, 830, 1595
NaeI	GCCGGC	1 cut(s)	48
NciI	CCSGG	1 cut(s)	275
NgoMIV	GCCGGC	1 cut(s)	46
NlaIV	GGNNCC	1 cut(s)	1555
NmeAIII	GCCGAG	1 cut(s)	403
NmuCI	GTSAC	2 cut(s)	343, 1661
NspV	TTCGAA	1 cut(s)	1487
PaeR7I	CTCGAG	1 cut(s)	233
PagI	TCATGA	1 cut(s)	126
PauI	GCGCGC	1 cut(s)	594
PciSI	GCTCTTC	1 cut(s)	167
PcsI	WCGNNNNNNNCGW	1 cut(s)	410
PctI	GAATGC	1 cut(s)	2151
PdiI	GCCGGC	1 cut(s)	48
PfeI	GAWTC	9 cut(s)	254, 354, 397, 406, 415, 1651, 1838, 2169, 2282
PfoI	TCCNGGA	3 cut(s)	273, 1526, 1671
PkrI	GCNGC	8 cut(s)	21, 341, 660, 835, 838, 1576, 1794, 2617
Ple19I	CGATCG	1 cut(s)	529
PleI	GAGTC	9 cut(s)	710, 987, 1000, 1081, 1684, 2569, 2615, 2719, 2751
PpsI	GAGTC	9 cut(s)	710, 987, 1000, 1081, 1684, 2569, 2615, 2719, 2751
PpuMI	RGGWCCY	1 cut(s)	1554
PshBI	ATTAAT	1 cut(s)	909
Psp124BI	GAGCTC	1 cut(s)	1443
Psp5II	RGGWCCY	1 cut(s)	1554
Psp6I	CCWGG	4 cut(s)	51, 1036, 1526, 1671
PspFI	CCCAGC	1 cut(s)	156
PspGI	CCWGG	4 cut(s)	51, 1036, 1526, 1671
PspN4I	GGNNCC	1 cut(s)	1555
PspPI	GGNCC	8 cut(s)	25, 49, 199, 969, 1189, 1554, 1726, 2780
PspPPI	RGGWCCY	1 cut(s)	1554
PsrI	GAACNNNNNNTAC	2 cut(s)	1103, 1135
PstI	CTGCAG	1 cut(s)	838
PstNI	CAGNNNCTG	2 cut(s)	502, 1132
PsuI	RGATCY	1 cut(s)	1616
PteI	GCGCGC	1 cut(s)	594
PvuI	CGATCG	1 cut(s)	529
RsaI	GTAC	6 cut(s)	332, 728, 1351, 1379, 1870, 2509
RsaNI	GTAC	6 cut(s)	331, 727, 1350, 1378, 1869, 2508
RseI	CAYNNNNRTG	1 cut(s)	470
SacI	GAGCTC	1 cut(s)	1443
SacII	CCGCGG	1 cut(s)	340
SapI	GCTCTTC	1 cut(s)	167
SatI	GCNGC	8 cut(s)	20, 340, 659, 834, 837, 1575, 1793, 2616
Sau96I	GGNCC	8 cut(s)	25, 49, 199, 969, 1189, 1554, 1726, 2780
ScaI	AGTACT	2 cut(s)	1379, 2509
SchI	GAGTC	9 cut(s)	711, 988, 1000, 1081, 1685, 2569, 2616, 2719, 2752
ScrFI	CCNGG	5 cut(s)	53, 275, 1038, 1528, 1673
SduI	GDGCHC	6 cut(s)	44, 323, 426, 966, 1184, 1443
SfaNI	GCATC	5 cut(s)	499, 1119, 1872, 2122, 2641
SfcI	CTRYAG	3 cut(s)	834, 1536, 1629
Sfr274I	CTCGAG	1 cut(s)	233
Sfr303I	CCGCGG	1 cut(s)	340
SfuI	TTCGAA	1 cut(s)	1487
SgrBI	CCGCGG	1 cut(s)	340
SinI	GGWCC	5 cut(s)	25, 199, 1189, 1554, 1726
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	1 cut(s)	470
SmlI	CTYRAG	9 cut(s)	233, 917, 1103, 1639, 1842, 2036, 2063, 2375, 2727
SmoI	CTYRAG	9 cut(s)	233, 917, 1103, 1639, 1842, 2036, 2063, 2375, 2727
SsiI	CCGC	9 cut(s)	178, 304, 337, 339, 427, 485, 1172, 1455, 2149
SspI	AATATT	1 cut(s)	1045
SspMI	CTAG	3 cut(s)	1433, 1718, 2273
SstI	GAGCTC	1 cut(s)	1443
StyD4I	CCNGG	5 cut(s)	51, 273, 1036, 1526, 1671
TaaI	ACNGT	7 cut(s)	208, 851, 1186, 1382, 1519, 2386, 2512
TaiI	ACGT	4 cut(s)	93, 186, 2101, 2366
TatI	WGTACW	5 cut(s)	330, 726, 1349, 1377, 2507
TauI	GCSGC	1 cut(s)	342
TfiI	GAWTC	9 cut(s)	254, 354, 397, 406, 415, 1651, 1838, 2169, 2282
TscAI	CASTG	6 cut(s)	772, 826, 1433, 1584, 2595, 2745
TseFI	GTSAC	2 cut(s)	343, 1661
TseI	GCWGC	7 cut(s)	19, 658, 833, 836, 1574, 1792, 2615
Tsp45I	GTSAC	2 cut(s)	343, 1661
TspDTI	ATGAA	6 cut(s)	128, 154, 1140, 1902, 2430, 2579
TspGWI	ACGGA	4 cut(s)	390, 460, 1348, 1856
TspRI	CASTG	6 cut(s)	772, 826, 1433, 1584, 2595, 2745
Vha464I	CTTAAG	2 cut(s)	917, 2036
VpaK11BI	GGWCC	5 cut(s)	25, 199, 1189, 1554, 1726
VspI	ATTAAT	1 cut(s)	909
XapI	RAATTY	2 cut(s)	1492, 2206
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1795
XhoI	CTCGAG	1 cut(s)	233
XspI	CTAG	3 cut(s)	1433, 1718, 2273
ZraI	GACGTC	1 cut(s)	2364
ZrmI	AGTACT	2 cut(s)	1379, 2509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.