MD14G1033800.v1.1

domain in TBC and LysM domain containing proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008522
Physical Location & Seq
Reverse (-)
2988194 .. 2991586
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1033800.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGGGAACTCTCAGTCACCTCCTTCTCGCAATCCTCGCTTTGCTTCCTCCTCTAGAGCTTTTACTCATACGGAACTCGAAGATCTCAAATCCTTGCACGCGTCTCTGGCTGCTCAGTCGCAGAGCGGTGGTCCGTACATCTCCCCCTCAGTTTTCAAGACTTATTTCGGACTAAAGGGAGCTCTCGGAGATAGGATGTTCGACTTAGTTACCCAGCAACGCAAAGACGGAAAGCTCACCTTTGAAGACTTAGTTATTGCCAAAGGGATTTATGAGAAGGGAACGAAAGATGACATTGAAGAATTCATATATCAGTTGTTAGACGTATCCGGGGATGGCATTGTGGGAAGGTCGGATTTGGAATGTGTTCTGGGTGCAATGTTTGACAATATATTCAATATGGACAACCCTGAACGTGGATCAAGTGCACATCTGGGCAGTGTCGATGTATTTCTTAATGCTGCAAAGTTTTCTAAGCATGACGAAGGACATGATCAAGAAAGTTTGTCTCTTGAAGATTTCAGAACTTGGTGTACTCTTCTCCCATCTGTCAGGAAGTTCCTTGGAAGCTTACTGATACCTCCTCATCAAGGAAGACCTGGTTCCCATGTTCCTCGACTAATGCATGTGGAAAACATTGATTCAAATATGGTGTTACTGAAGGAGGTATATGCTTGGCATATAGGAGGAATCCTTCCACAACAAGAGCTGGTGGAATGGAAACTGCTATATCACAGTTCTTTTAGCGGTCTGAGCTTTAACACCTTTTTGGGCAGCATGTCAAATGATAAAGGACCAACTGTGTTAATCATCAAGGATAAAGATGGTTATATTTATGGGGGTTACGCTTCCCAGCCATGGGAGAGGCACGGTGGTTTCTATGGAGATCTGAAGTCTTTTCTTTTCCAGCTGTATCCGAAGGCGTCAATATTCAGGCCAACAGGGGCAAACACCAACATACAATGGTGTGCTGTGAATTTCAGTTCAGAGAGCATCCCGAATGGTATCGGTTTTGGAGGAAAGGTGAATCACTTTGGCCTTTTTCTTTCGGCCAGCTTTGATCAGGGGCACACTTTCTCCTGTACCACATTTGGCTCCCCTTGCCTCTCCAAGACCAACCGGATACACCCGGAAGTGATAGAATGCTGGGGAGTTGTTACCAAATTAGCAGAACAAGAAAAACACGCCGGTGGAAAAGGTAATGTGCTGGAGAGATTTAAGGAAGACCGCCATATGCTTAACATGGTTGGGCTTGCTAATTCAAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

424

Amino Acids

46.84

Weight (kDa)

6.15

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 217 - 385 4.2e-43 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 1 cut(s) 101
AciI CCGC 3 cut(s) 126, 747, 1228
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 1050
AcsI RAATTY 2 cut(s) 302, 976
AcuI CTGAAG 2 cut(s) 680, 911
AcyI GRCGYC 1 cut(s) 923
AfaI GTAC 3 cut(s) 137, 535, 1084
AfiI CCNNNNNNNGG 4 cut(s) 416, 590, 858, 859
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 7 cut(s) 157, 245, 299, 397, 515, 645, 1263
AjnI CCWGG 1 cut(s) 598
AleI CACNNNNGTG 1 cut(s) 1188
AluBI AGCT 8 cut(s) 59, 182, 235, 570, 709, 756, 910, 1056
AluI AGCT 8 cut(s) 59, 182, 235, 570, 709, 756, 910, 1056
Alw21I GWGCWC 2 cut(s) 184, 430
Alw26I GTCTC 2 cut(s) 108, 513
Alw44I GTGCAC 1 cut(s) 426
AlwI GGATC 1 cut(s) 427
AoxI GGCC 3 cut(s) 935, 1036, 1050
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 3 cut(s) 110, 461, 774
ApoI RAATTY 2 cut(s) 302, 976
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 131, 794
AsuC2I CCSGG 2 cut(s) 331, 1130
AsuHPI GGTGA 3 cut(s) 9, 229, 1036
AvaII GGWCC 2 cut(s) 131, 794
BaeGI GKGCMC 2 cut(s) 430, 1071
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 3 cut(s) 252, 601, 1230
Bbv12I GWGCWC 2 cut(s) 184, 430
BbvI GCAGC 3 cut(s) 97, 448, 786
BccI CCATC 3 cut(s) 329, 553, 818
BciT130I CCWGG 1 cut(s) 600
BciVI GTATCC 3 cut(s) 337, 924, 1116
BclI TGATCA 2 cut(s) 493, 1060
BcnI CCSGG 2 cut(s) 331, 1130
BcoDI GTCTC 2 cut(s) 108, 513
BfaI CTAG 1 cut(s) 54
BfuI GTATCC 3 cut(s) 337, 924, 1116
BglII AGATCT 2 cut(s) 82, 886
BisI GCNGC 3 cut(s) 111, 462, 775
BlsI GCNGC 3 cut(s) 112, 463, 776
Bme1390I CCNGG 3 cut(s) 331, 600, 1130
Bme18I GGWCC 2 cut(s) 131, 794
BmgT120I GGNCC 2 cut(s) 131, 794
BmiI GGNNCC 2 cut(s) 604, 1096
BmrFI CCNGG 3 cut(s) 331, 600, 1130
BmsI GCATC 1 cut(s) 1002
BpiI GAAGAC 3 cut(s) 252, 601, 1230
BpmI CTGGAG 1 cut(s) 1229
BpuMI CCSGG 2 cut(s) 331, 1130
BsaHI GRCGYC 1 cut(s) 923
BsaJI CCNNGG 3 cut(s) 330, 562, 857
BsaWI WCCGGW 1 cut(s) 1119
BsaXI ACNNNNNCTCC 4 cut(s) 1008, 1038, 1061, 1091
Bsc4I CCNNNNNNNGG 4 cut(s) 416, 590, 858, 859
Bse118I RCCGGY 1 cut(s) 1187
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 330, 562, 857
BseGI GGATG 3 cut(s) 201, 340, 993
BseLI CCNNNNNNNGG 4 cut(s) 416, 590, 858, 859
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 4 cut(s) 26, 128, 162, 743
BseRI GAGGAG 2 cut(s) 40, 573
BseSI GKGCMC 2 cut(s) 430, 1071
BseXI GCAGC 3 cut(s) 97, 448, 786
BseYI CCCAGC 3 cut(s) 213, 852, 1146
Bsh1236I CGCG 1 cut(s) 101
BshFI GGCC 3 cut(s) 937, 1038, 1052
BsiHKAI GWGCWC 2 cut(s) 184, 430
BsiSI CCGG 4 cut(s) 330, 1120, 1130, 1188
BslI CCNNNNNNNGG 4 cut(s) 416, 590, 858, 859
BsmAI GTCTC 2 cut(s) 108, 513
BsmBI CGTCTC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 1148
BsnI GGCC 3 cut(s) 937, 1038, 1052
Bsp1286I GDGCHC 3 cut(s) 184, 430, 1071
Bsp143I GATC 5 cut(s) 82, 419, 493, 886, 1060
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 3 cut(s) 126, 747, 1228
BspANI GGCC 3 cut(s) 937, 1038, 1052
BspCNI CTCAG 4 cut(s) 25, 127, 161, 744
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 2 cut(s) 604, 1096
BspPI GGATC 1 cut(s) 427
BsrBI CCGCTC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 1187
BssAI RCCGGY 1 cut(s) 1187
BssECI CCNNGG 3 cut(s) 330, 562, 857
BssMI GATC 5 cut(s) 82, 419, 493, 886, 1060
BssNI GRCGYC 1 cut(s) 923
BssT1I CCWWGG 2 cut(s) 562, 857
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 3 cut(s) 737, 802, 872
Bst6I CTCTTC 1 cut(s) 543
BstACI GRCGYC 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 99, 1054, 1254
BstDEI CTNAG 7 cut(s) 12, 114, 148, 205, 250, 474, 752
BstDSI CCRYGG 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 3 cut(s) 201, 340, 993
BstFNI CGCG 1 cut(s) 101
BstKTI GATC 5 cut(s) 85, 422, 496, 889, 1063
BstMAI GTCTC 2 cut(s) 108, 513
BstMBI GATC 5 cut(s) 82, 419, 493, 886, 1060
BstMWI GCNNNNNNNGC 4 cut(s) 36, 107, 753, 1101
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 2 cut(s) 629, 781
BstSCI CCNGG 3 cut(s) 329, 598, 1128
BstSLI GKGCMC 2 cut(s) 430, 1071
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 3 cut(s) 97, 448, 786
BstV2I GAAGAC 3 cut(s) 252, 601, 1230
BstX2I RGATCY 2 cut(s) 82, 886
BstYI RGATCY 2 cut(s) 82, 886
BsuI GTATCC 3 cut(s) 337, 924, 1116
BsuRI GGCC 3 cut(s) 937, 1038, 1052
BtgI CCRYGG 1 cut(s) 857
BtsCI GGATG 3 cut(s) 201, 340, 993
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 99, 1054, 1254
Cfr10I RCCGGY 1 cut(s) 1187
Cfr13I GGNCC 2 cut(s) 131, 794
CseI GACGC 2 cut(s) 90, 912
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 3 cut(s) 136, 534, 1083
CviAII CATG 7 cut(s) 479, 491, 608, 626, 778, 858, 1243
CviQI GTAC 3 cut(s) 136, 534, 1083
DdeI CTNAG 7 cut(s) 12, 114, 148, 205, 250, 474, 752
DpnI GATC 5 cut(s) 84, 421, 495, 888, 1062
DpnII GATC 5 cut(s) 82, 419, 493, 886, 1060
EaeI YGGCCR 1 cut(s) 1050
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 2 cut(s) 562, 857
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 131, 794
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 2 cut(s) 680, 911
EcoICRI GAGCTC 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 598
EcoT14I CCWWGG 2 cut(s) 562, 857
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 562, 857
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 7 cut(s) 482, 494, 611, 629, 781, 861, 1246
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 7 cut(s) 478, 490, 607, 625, 777, 857, 1242
FauNDI CATATG 1 cut(s) 1233
FbaI TGATCA 2 cut(s) 493, 1060
Fnu4HI GCNGC 3 cut(s) 111, 462, 775
FokI GGATG 3 cut(s) 208, 347, 980
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 3 cut(s) 111, 462, 775
FspBI CTAG 1 cut(s) 54
GluI GCNGC 3 cut(s) 111, 462, 775
GsaI CCCAGC 3 cut(s) 217, 856, 1150
GsuI CTGGAG 1 cut(s) 1229
HaeIII GGCC 3 cut(s) 937, 1038, 1052
HapII CCGG 4 cut(s) 330, 1120, 1130, 1188
HgaI GACGC 2 cut(s) 90, 912
Hin1I GRCGYC 1 cut(s) 923
Hin1II CATG 7 cut(s) 482, 494, 611, 629, 781, 861, 1246
HindIII AAGCTT 1 cut(s) 568
HinfI GANTC 3 cut(s) 641, 690, 1027
HpaII CCGG 4 cut(s) 330, 1120, 1130, 1188
HphI GGTGA 3 cut(s) 9, 229, 1036
Hpy166II GTNNAC 2 cut(s) 428, 534
Hpy188I TCNGA 8 cut(s) 170, 188, 355, 524, 753, 891, 918, 988
Hpy188III TCNNGA 6 cut(s) 54, 157, 497, 512, 553, 997
Hpy8I GTNNAC 2 cut(s) 428, 534
HpyAV CCTTC 7 cut(s) 33, 271, 342, 479, 655, 704, 913
HpyCH4III ACNGT 3 cut(s) 737, 802, 872
HpyCH4IV ACGT 2 cut(s) 324, 415
HpyCH4V TGCA 5 cut(s) 97, 377, 428, 464, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 107, 753, 1101
HpyF3I CTNAG 7 cut(s) 12, 114, 148, 205, 250, 474, 752
HpySE526I ACGT 2 cut(s) 324, 415
Hsp92I GRCGYC 1 cut(s) 923
Hsp92II CATG 7 cut(s) 482, 494, 611, 629, 781, 861, 1246
Ksp22I TGATCA 2 cut(s) 493, 1060
Kzo9I GATC 5 cut(s) 82, 419, 493, 886, 1060
LmnI GCTCC 2 cut(s) 179, 1100
Lsp1109I GCAGC 3 cut(s) 97, 448, 786
LweI GCATC 1 cut(s) 1002
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 1 cut(s) 54
MaeII ACGT 2 cut(s) 324, 415
MaeIII GTNAC 5 cut(s) 15, 208, 654, 842, 1156
MalI GATC 5 cut(s) 84, 421, 495, 888, 1062
MbiI CCGCTC 1 cut(s) 126
MboI GATC 5 cut(s) 82, 419, 493, 886, 1060
MboII GAAGA 7 cut(s) 92, 257, 311, 527, 530, 606, 1235
MflI RGATCY 2 cut(s) 82, 886
MhlI GDGCHC 3 cut(s) 184, 430, 1071
MluCI AATT 4 cut(s) 302, 976, 1163, 1258
MluI ACGCGT 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 333
Mph1103I ATGCAT 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 5 cut(s) 456, 759, 806, 1218, 1239
MslI CAYNNNNRTG 1 cut(s) 1188
MspA1I CMGCKG 1 cut(s) 910
MspI CCGG 4 cut(s) 330, 1120, 1130, 1188
MspR9I CCNGG 3 cut(s) 331, 600, 1130
Mva1269I GAATGC 1 cut(s) 1148
MvaI CCWGG 1 cut(s) 600
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 4 cut(s) 36, 107, 753, 1101
NciI CCSGG 2 cut(s) 331, 1130
NcoI CCATGG 1 cut(s) 857
NdeI CATATG 1 cut(s) 1233
NdeII GATC 5 cut(s) 82, 419, 493, 886, 1060
NlaIII CATG 7 cut(s) 482, 494, 611, 629, 781, 861, 1246
NlaIV GGNNCC 2 cut(s) 604, 1096
NmuCI GTSAC 1 cut(s) 15
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 629, 781
OliI CACNNNNGTG 1 cut(s) 1188
PctI GAATGC 1 cut(s) 1148
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 641, 690, 1027
PkrI GCNGC 3 cut(s) 112, 463, 776
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 598
PspFI CCCAGC 3 cut(s) 213, 852, 1146
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 2 cut(s) 604, 1096
PspPI GGNCC 2 cut(s) 131, 794
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PsuI RGATCY 2 cut(s) 82, 886
PvuII CAGCTG 1 cut(s) 910
RsaI GTAC 3 cut(s) 137, 535, 1084
RsaNI GTAC 3 cut(s) 136, 534, 1083
RseI CAYNNNNRTG 1 cut(s) 1188
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 5 cut(s) 456, 759, 806, 1218, 1239
SatI GCNGC 3 cut(s) 111, 462, 775
Sau3AI GATC 5 cut(s) 82, 419, 493, 886, 1060
Sau96I GGNCC 2 cut(s) 131, 794
ScrFI CCNGG 3 cut(s) 331, 600, 1130
SduI GDGCHC 3 cut(s) 184, 430, 1071
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 1 cut(s) 1002
SgrAI CRCCGGYG 1 cut(s) 1187
SinI GGWCC 2 cut(s) 131, 794
SmiMI CAYNNNNRTG 1 cut(s) 1188
Sse9I AATT 4 cut(s) 302, 976, 1163, 1258
SsiI CCGC 3 cut(s) 126, 747, 1228
SspI AATATT 1 cut(s) 930
SspMI CTAG 1 cut(s) 54
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 3 cut(s) 329, 598, 1128
StyI CCWWGG 2 cut(s) 562, 857
TaaI ACNGT 3 cut(s) 737, 802, 872
TaiI ACGT 2 cut(s) 327, 418
TaqI TCGA 4 cut(s) 78, 201, 444, 616
TasI AATT 4 cut(s) 302, 976, 1163, 1258
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 3 cut(s) 641, 690, 1027
Tru1I TTAA 5 cut(s) 456, 759, 806, 1218, 1239
Tru9I TTAA 5 cut(s) 456, 759, 806, 1218, 1239
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 3 cut(s) 110, 461, 774
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 3 cut(s) 86, 123, 243
TspRI CASTG 1 cut(s) 445
VneI GTGCAC 1 cut(s) 426
VpaK11BI GGWCC 2 cut(s) 131, 794
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 2 cut(s) 302, 976
XbaI TCTAGA 1 cut(s) 53
XceI RCATGY 2 cut(s) 629, 781
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.