pycom14g02980

domain in TBC and LysM domain containing proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008522
Physical Location & Seq
Reverse (-)
2288088 .. 2291199
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02980.1

Sequence Viewer

Length: 1347 bp
ATGAAAAAGTATAAATATCAATCTAGATTTTTTCGGAGTCCACACTGCAGAGGGAAGAAGAAAACCAGAAAAAAAATGGGGAACTCTCAGTCACCTCCTTCTCACAATCCTCGCTTTGCTTCCTCCTCTAGAGCTTTTACTCATACGGAACTCGAAGATCTCAAATCCCTGCACGCGTCTCTGGCTGCTAAGTCGCAGAGCGGTGGTCCGTACATCTCCCCCTCAGTTTTTAATACTTATTTCGGACTAAAGGGAGCTCTGGGAGATAGGATGTTCGACTTAGTTACCCAGCAACGCAAAAACGGAAAGCTCACCTTTGAAGACTTAGTTATTGCCAAAGGGATTTATGAGAAGGGAACGAAAGATGACATTGAAGAATTCATATATCAGTTGTTAGACGTATCCGGGGATGGCATTGTGGGAAGGTCGGATTTGGAATGTGTTCTGGGTGCATTGTTTGACAATATATTCAATATGGACACCCCTGAACGTGGATCAAGTGCACATCAGGGCAGTGTCGATGTATTTCTTAATGCTGCAAAGTTTACTAAGCATGACGAAGGACATGATCAAGAAAGTTTGTCTCTTGAAGATTTCAGAACTTGGTGTACTCTTCTCCCATCTGTCAGGAAGTTCCTTGGAAGCTTATTGATACCTCCTCATCAAGGAAGACCAGGTTCCCAAGTTCCTCGACTAATGCATGTGGAAAACATCGATTCAAATATGGTGTTACTGAAGGAGGTATATGCTTGGCATATAGGAGGAATCCTTCCACAACAAGAGCTGGTGGAATGGAAACTGCTATACCACAGTTCTTTTAGCGGTCTGAGCTTTAACACCTTTTTGGGCAGCGTGTCAAATGATAAGGGACCAACTGTGTTAATCATCAAGGATAAAGATGGTTATATTTATGGGGGTTACGCTTCCCAGCCATGGGAGAGGCATGGTGGTTTCTATGGAGATCTGAAGTCTTTTCTTTTCCAGCTGTATCCGAAGGCGTCAATATTCAGGCCAACGGGGGCAAACACCAACATACAATGGTGTGCTGTGAATTTCAGTTCAGAGAGCATCCCGAATGGTATCGGTTTTGGAGGAAAGGTGAATCACTTTGGCCTTTTTCTTTCAGCCAGCTTTGATCAGGGGCACACTTTCTCCTGTACCACATTTGGCTCCCCTTGCCTCTCCAACACCAACCGGATACACCCGGAAGTTATAGAATGCTGGGGAGTTGTTACCAAAGTAGCAGAACAAGAAAAACACGCCGGTGGAAAAGGTAATGTACTGGAGAGATTTAAGGAAGACCGCCATATGCTTAACATGGTTGGGCTTGCTAATTCAAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

449

Amino Acids

49.93

Weight (kDa)

8.51

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 242 - 410 3.6e-43 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 201, 822, 1303
AclWI GGATC 1 cut(s) 502
AcsI RAATTY 2 cut(s) 377, 1051
AcuI CTGAAG 2 cut(s) 755, 986
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 4 cut(s) 212, 610, 1159, 1281
AfiI CCNNNNNNNGG 4 cut(s) 491, 665, 933, 934
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 6 cut(s) 320, 374, 472, 590, 720, 1338
AjnI CCWGG 1 cut(s) 673
AleI CACNNNNGTG 1 cut(s) 1263
AluBI AGCT 8 cut(s) 134, 257, 310, 645, 784, 831, 985, 1131
AluI AGCT 8 cut(s) 134, 257, 310, 645, 784, 831, 985, 1131
Alw21I GWGCWC 2 cut(s) 259, 505
Alw26I GTCTC 2 cut(s) 183, 588
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 1 cut(s) 502
AoxI GGCC 2 cut(s) 1010, 1111
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 3 cut(s) 185, 536, 849
ApoI RAATTY 2 cut(s) 377, 1051
Asp700I GAANNNNTTC 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 206, 869
AsuC2I CCSGG 2 cut(s) 406, 1205
AsuHPI GGTGA 3 cut(s) 84, 304, 1111
AvaII GGWCC 2 cut(s) 206, 869
BaeGI GKGCMC 2 cut(s) 505, 1146
BanII GRGCYC 1 cut(s) 259
BbsI GAAGAC 3 cut(s) 327, 676, 1305
Bbv12I GWGCWC 2 cut(s) 259, 505
BbvI GCAGC 3 cut(s) 172, 523, 861
BccI CCATC 3 cut(s) 404, 628, 893
BciT130I CCWGG 1 cut(s) 675
BciVI GTATCC 3 cut(s) 412, 999, 1191
BclI TGATCA 2 cut(s) 568, 1135
BcnI CCSGG 2 cut(s) 406, 1205
BcoDI GTCTC 2 cut(s) 183, 588
BfaI CTAG 2 cut(s) 24, 129
BfmI CTRYAG 1 cut(s) 46
BfuI GTATCC 3 cut(s) 412, 999, 1191
BglII AGATCT 2 cut(s) 157, 961
BisI GCNGC 3 cut(s) 186, 537, 850
BlsI GCNGC 3 cut(s) 187, 538, 851
Bme1390I CCNGG 3 cut(s) 406, 675, 1205
Bme18I GGWCC 2 cut(s) 206, 869
BmgT120I GGNCC 2 cut(s) 206, 869
BmiI GGNNCC 3 cut(s) 679, 870, 1171
BmrFI CCNGG 3 cut(s) 406, 675, 1205
BmsI GCATC 1 cut(s) 1077
BpiI GAAGAC 3 cut(s) 327, 676, 1305
BpmI CTGGAG 1 cut(s) 1304
BpuMI CCSGG 2 cut(s) 406, 1205
Bsa29I ATCGAT 1 cut(s) 714
BsaHI GRCGYC 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 405, 637, 932
BsaWI WCCGGW 1 cut(s) 1194
BsaXI ACNNNNNCTCC 4 cut(s) 1083, 1113, 1136, 1166
Bsc4I CCNNNNNNNGG 4 cut(s) 491, 665, 933, 934
Bse118I RCCGGY 1 cut(s) 1262
Bse1I ACTGG 1 cut(s) 1287
BseBI CCWGG 1 cut(s) 675
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 3 cut(s) 405, 637, 932
BseGI GGATG 3 cut(s) 276, 415, 1068
BseLI CCNNNNNNNGG 4 cut(s) 491, 665, 933, 934
BseMII CTCAG 3 cut(s) 101, 237, 818
BseNI ACTGG 1 cut(s) 1287
BseRI GAGGAG 2 cut(s) 115, 648
BseSI GKGCMC 2 cut(s) 505, 1146
BseXI GCAGC 3 cut(s) 172, 523, 861
BseYI CCCAGC 3 cut(s) 288, 927, 1221
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 1012, 1113
BshVI ATCGAT 1 cut(s) 714
BsiHKAI GWGCWC 2 cut(s) 259, 505
BsiSI CCGG 4 cut(s) 405, 1195, 1205, 1263
BslFI GGGAC 1 cut(s) 882
BslI CCNNNNNNNGG 4 cut(s) 491, 665, 933, 934
BsmAI GTCTC 2 cut(s) 183, 588
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 882
BsmI GAATGC 1 cut(s) 1223
BsnI GGCC 2 cut(s) 1012, 1113
Bsp1286I GDGCHC 3 cut(s) 259, 505, 1146
Bsp143I GATC 5 cut(s) 157, 494, 568, 961, 1135
Bsp19I CCATGG 1 cut(s) 932
BspACI CCGC 3 cut(s) 201, 822, 1303
BspANI GGCC 2 cut(s) 1012, 1113
BspCNI CTCAG 3 cut(s) 100, 236, 819
BspDI ATCGAT 1 cut(s) 714
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 679, 870, 1171
BspMAI CTGCAG 1 cut(s) 50
BspPI GGATC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 1262
BsrI ACTGG 1 cut(s) 1287
BssAI RCCGGY 1 cut(s) 1262
BssECI CCNNGG 3 cut(s) 405, 637, 932
BssMI GATC 5 cut(s) 157, 494, 568, 961, 1135
BssNI GRCGYC 1 cut(s) 998
BssT1I CCWWGG 2 cut(s) 637, 932
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 2 cut(s) 812, 877
Bst6I CTCTTC 1 cut(s) 618
BstACI GRCGYC 1 cut(s) 998
BstC8I GCNNGC 3 cut(s) 174, 1129, 1329
BstDEI CTNAG 7 cut(s) 87, 189, 223, 280, 325, 549, 827
BstDSI CCRYGG 1 cut(s) 932
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 3 cut(s) 276, 415, 1068
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 5 cut(s) 160, 497, 571, 964, 1138
BstMAI GTCTC 2 cut(s) 183, 588
BstMBI GATC 5 cut(s) 157, 494, 568, 961, 1135
BstMWI GCNNNNNNNGC 3 cut(s) 182, 828, 1176
BstNI CCWGG 1 cut(s) 675
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 3 cut(s) 404, 673, 1203
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 2 cut(s) 505, 1146
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 172, 523, 861
BstV2I GAAGAC 3 cut(s) 327, 676, 1305
BstX2I RGATCY 2 cut(s) 157, 961
BstYI RGATCY 2 cut(s) 157, 961
Bsu15I ATCGAT 1 cut(s) 714
BsuI GTATCC 3 cut(s) 412, 999, 1191
BsuRI GGCC 2 cut(s) 1012, 1113
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 1 cut(s) 932
BtsCI GGATG 3 cut(s) 276, 415, 1068
BtsI GCAGTG 2 cut(s) 43, 520
BtsIMutI CAGTG 2 cut(s) 43, 520
Cac8I GCNNGC 3 cut(s) 174, 1129, 1329
Cfr10I RCCGGY 1 cut(s) 1262
Cfr13I GGNCC 2 cut(s) 206, 869
ClaI ATCGAT 1 cut(s) 714
CseI GACGC 2 cut(s) 165, 987
CsiI ACCWGGT 1 cut(s) 673
Csp6I GTAC 4 cut(s) 211, 609, 1158, 1280
CviAII CATG 6 cut(s) 554, 566, 701, 933, 944, 1318
CviQI GTAC 4 cut(s) 211, 609, 1158, 1280
DdeI CTNAG 7 cut(s) 87, 189, 223, 280, 325, 549, 827
DpnI GATC 5 cut(s) 159, 496, 570, 963, 1137
DpnII GATC 5 cut(s) 157, 494, 568, 961, 1135
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 2 cut(s) 637, 932
Eco24I GRGCYC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 206, 869
Eco53kI GAGCTC 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 755, 986
EcoICRI GAGCTC 1 cut(s) 257
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 2 cut(s) 637, 932
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 2 cut(s) 637, 932
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 6 cut(s) 557, 569, 704, 936, 947, 1321
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 882
FatI CATG 6 cut(s) 553, 565, 700, 932, 943, 1317
FauNDI CATATG 1 cut(s) 1308
FbaI TGATCA 2 cut(s) 568, 1135
Fnu4HI GCNGC 3 cut(s) 186, 537, 850
FokI GGATG 3 cut(s) 283, 422, 1055
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 3 cut(s) 186, 537, 850
FspBI CTAG 2 cut(s) 24, 129
GluI GCNGC 3 cut(s) 186, 537, 850
GsaI CCCAGC 3 cut(s) 292, 931, 1225
GsuI CTGGAG 1 cut(s) 1304
HaeIII GGCC 2 cut(s) 1012, 1113
HapII CCGG 4 cut(s) 405, 1195, 1205, 1263
HgaI GACGC 2 cut(s) 165, 987
Hin1I GRCGYC 1 cut(s) 998
Hin1II CATG 6 cut(s) 557, 569, 704, 936, 947, 1321
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 4 cut(s) 37, 716, 765, 1102
HpaII CCGG 4 cut(s) 405, 1195, 1205, 1263
HphI GGTGA 3 cut(s) 84, 304, 1111
Hpy166II GTNNAC 4 cut(s) 41, 503, 546, 609
Hpy188I TCNGA 8 cut(s) 36, 245, 430, 599, 828, 966, 993, 1063
Hpy188III TCNNGA 6 cut(s) 24, 129, 572, 587, 628, 1072
Hpy8I GTNNAC 4 cut(s) 41, 503, 546, 609
HpyAV CCTTC 7 cut(s) 108, 346, 417, 554, 730, 779, 988
HpyCH4III ACNGT 2 cut(s) 812, 877
HpyCH4IV ACGT 2 cut(s) 399, 490
HpyCH4V TGCA 6 cut(s) 48, 172, 452, 503, 539, 700
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 828, 1176
HpyF3I CTNAG 7 cut(s) 87, 189, 223, 280, 325, 549, 827
HpySE526I ACGT 2 cut(s) 399, 490
Hsp92I GRCGYC 1 cut(s) 998
Hsp92II CATG 6 cut(s) 557, 569, 704, 936, 947, 1321
Ksp22I TGATCA 2 cut(s) 568, 1135
Kzo9I GATC 5 cut(s) 157, 494, 568, 961, 1135
LmnI GCTCC 2 cut(s) 254, 1175
Lsp1109I GCAGC 3 cut(s) 172, 523, 861
LweI GCATC 1 cut(s) 1077
MabI ACCWGGT 1 cut(s) 673
MaeI CTAG 2 cut(s) 24, 129
MaeII ACGT 2 cut(s) 399, 490
MaeIII GTNAC 5 cut(s) 90, 283, 729, 917, 1231
MalI GATC 5 cut(s) 159, 496, 570, 963, 1137
MbiI CCGCTC 1 cut(s) 201
MboI GATC 5 cut(s) 157, 494, 568, 961, 1135
MboII GAAGA 9 cut(s) 67, 70, 167, 332, 386, 602, 605, 681, 1310
MflI RGATCY 2 cut(s) 157, 961
MhlI GDGCHC 3 cut(s) 259, 505, 1146
MluCI AATT 3 cut(s) 377, 1051, 1333
MluI ACGCGT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 2 cut(s) 408, 1209
Mph1103I ATGCAT 1 cut(s) 702
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 6 cut(s) 231, 531, 834, 881, 1293, 1314
MslI CAYNNNNRTG 1 cut(s) 1263
MspA1I CMGCKG 1 cut(s) 985
MspI CCGG 4 cut(s) 405, 1195, 1205, 1263
MspR9I CCNGG 3 cut(s) 406, 675, 1205
Mva1269I GAATGC 1 cut(s) 1223
MvaI CCWGG 1 cut(s) 675
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 182, 828, 1176
NciI CCSGG 2 cut(s) 406, 1205
NcoI CCATGG 1 cut(s) 932
NdeI CATATG 1 cut(s) 1308
NdeII GATC 5 cut(s) 157, 494, 568, 961, 1135
NlaIII CATG 6 cut(s) 557, 569, 704, 936, 947, 1321
NlaIV GGNNCC 3 cut(s) 679, 870, 1171
NmuCI GTSAC 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 702
NspI RCATGY 1 cut(s) 704
OliI CACNNNNGTG 1 cut(s) 1263
PctI GAATGC 1 cut(s) 1223
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 3 cut(s) 716, 765, 1102
PkrI GCNGC 3 cut(s) 187, 538, 851
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 673
PspFI CCCAGC 3 cut(s) 288, 927, 1221
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 3 cut(s) 679, 870, 1171
PspPI GGNCC 2 cut(s) 206, 869
PsrI GAACNNNNNNTAC 2 cut(s) 592, 624
PstI CTGCAG 1 cut(s) 50
PsuI RGATCY 2 cut(s) 157, 961
PvuII CAGCTG 1 cut(s) 985
RsaI GTAC 4 cut(s) 212, 610, 1159, 1281
RsaNI GTAC 4 cut(s) 211, 609, 1158, 1280
RseI CAYNNNNRTG 1 cut(s) 1263
SacI GAGCTC 1 cut(s) 259
SaqAI TTAA 6 cut(s) 231, 531, 834, 881, 1293, 1314
SatI GCNGC 3 cut(s) 186, 537, 850
Sau3AI GATC 5 cut(s) 157, 494, 568, 961, 1135
Sau96I GGNCC 2 cut(s) 206, 869
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 3 cut(s) 406, 675, 1205
SduI GDGCHC 3 cut(s) 259, 505, 1146
SexAI ACCWGGT 1 cut(s) 673
SfaNI GCATC 1 cut(s) 1077
SfcI CTRYAG 1 cut(s) 46
SgrAI CRCCGGYG 1 cut(s) 1262
SinI GGWCC 2 cut(s) 206, 869
SmiMI CAYNNNNRTG 1 cut(s) 1263
Sse9I AATT 3 cut(s) 377, 1051, 1333
SsiI CCGC 3 cut(s) 201, 822, 1303
SspI AATATT 1 cut(s) 1005
SspMI CTAG 2 cut(s) 24, 129
SstI GAGCTC 1 cut(s) 259
StyD4I CCNGG 3 cut(s) 404, 673, 1203
StyI CCWWGG 2 cut(s) 637, 932
TaaI ACNGT 2 cut(s) 812, 877
TaiI ACGT 2 cut(s) 402, 493
TaqI TCGA 5 cut(s) 153, 276, 519, 691, 714
TasI AATT 3 cut(s) 377, 1051, 1333
TatI WGTACW 2 cut(s) 608, 1279
TfiI GAWTC 3 cut(s) 716, 765, 1102
Tru1I TTAA 6 cut(s) 231, 531, 834, 881, 1293, 1314
Tru9I TTAA 6 cut(s) 231, 531, 834, 881, 1293, 1314
TscAI CASTG 2 cut(s) 50, 520
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 3 cut(s) 185, 536, 849
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 2 cut(s) 17, 370
TspGWI ACGGA 3 cut(s) 161, 198, 318
TspRI CASTG 2 cut(s) 50, 520
VneI GTGCAC 1 cut(s) 501
VpaK11BI GGWCC 2 cut(s) 206, 869
XagI CCTNNNNNAGG 1 cut(s) 663
XapI RAATTY 2 cut(s) 377, 1051
XbaI TCTAGA 2 cut(s) 23, 128
XceI RCATGY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 24, 129
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.