Rh3DG222200

domain in TBC and LysM domain containing proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008522
Physical Location & Seq
Reverse (-)
20837000 .. 20840982
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG222200.1

Sequence Viewer

Length: 894 bp
ATGTTTGAATACATATTCAAAACAAAAAACGCTGAACCTGGATCAAGTACATATCGGGGAACTGTGAATGCATTTCTTAATGCTGCACGGCTTACAAAGCACGACGAAGGACATACTGAGGAAAGTTTTTCCTTTGAAGATTTCAGAACTTGGTGCACTCTTCTTCCTTCTGTCAGGAAGTTCCTTGGAAGCTTATTGATTCCACCTGATCAAGGAAGGCCAGGTTCTCAGGTTCCACGGTTATTGCAATTGGAAAACATTAATTCAAATATGATATTATTAAACGAGGTGTATGCCTGGCATATAGGAGGAATTCTTCCTCACCAAGAGCTGGGAGAATGGAAACTATTATACCATAGTGCTGTTAATGGTCTGAGCTTCAACACATTTGTGGGAAACATATCAAATGATAGAGGGCCAACTGTATTCATTATCAAGGATAAAGACGGTTATGTATATGGAGGTTATGCTTCCCAACCGTGGGAGAGGCATGGTGATTTCTATGGTGATCTGAAGTCATTTCTTTTCCAATTGTATCCGAAGGCATCTATATATAGGCCTACTGGAGCAAACACTAATCTACAATGGTGTGCTGTGAATTTCGGTTCAGAGAGCATCCCAAATGGTATTGGTTTTGGAGGAAAGGTGAATCACTTTGGTCTCTTCCTTTCGGCGAACTTTGATCAGGGGCACACTTTCTCGTGTACCACATTTGGTTCCCCTTGCCTCTCCAAGACCAGCCAAATATGCCCGGAAGTAATAGAATGCTGGGGAGTTGTAAGGAAAGCAGCAGATGAAGAAAAACTGGATGGTGCTAAAGGCACTGTATTGGAGAGGTTTAAGGAAGATCGTCATATGCTCAACATGGTTGGGCTCGCAAATTCCAGCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TLD

Protein Analysis

297

Amino Acids

33.19

Weight (kDa)

6.15

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TLD PF07534 89 - 259 7.3e-43 TLDc domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 331
AclWI GGATC 1 cut(s) 49
AcsI RAATTY 3 cut(s) 312, 598, 880
AcuI CTGAAG 1 cut(s) 533
AfaI GTAC 2 cut(s) 49, 706
AfiI CCNNNNNNNGG 4 cut(s) 212, 331, 480, 481
AgsI TTSAA 5 cut(s) 8, 19, 137, 267, 382
AjnI CCWGG 3 cut(s) 37, 220, 296
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 3 cut(s) 192, 331, 378
AluI AGCT 3 cut(s) 192, 331, 378
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 1 cut(s) 665
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 1 cut(s) 49
AoxI GGCC 3 cut(s) 218, 416, 557
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 83, 788
ApoI RAATTY 3 cut(s) 312, 598, 880
AseI ATTAAT 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 4 cut(s) 314, 506, 518, 658
BaeGI GKGCMC 2 cut(s) 158, 693
BanII GRGCYC 1 cut(s) 876
BauI CACGAG 1 cut(s) 700
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 2 cut(s) 70, 800
BccI CCATC 1 cut(s) 803
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 3 cut(s) 39, 222, 298
BciVI GTATCC 1 cut(s) 546
BclI TGATCA 2 cut(s) 208, 682
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 1 cut(s) 665
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 2 cut(s) 84, 789
BlsI GCNGC 2 cut(s) 85, 790
Bme1390I CCNGG 4 cut(s) 39, 222, 298, 752
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 234, 718
BmrFI CCNGG 4 cut(s) 39, 222, 298, 752
BmsI GCATC 2 cut(s) 554, 624
BpmI CTGGAG 1 cut(s) 585
BpuMI CCSGG 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 184, 236, 479
BsaXI ACNNNNNCTCC 2 cut(s) 630, 660
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 331, 480, 481
Bse1I ACTGG 2 cut(s) 568, 810
BseBI CCWGG 3 cut(s) 39, 222, 298
BseDI CCNNGG 3 cut(s) 184, 236, 479
BseGI GGATG 2 cut(s) 615, 814
BseLI CCNNNNNNNGG 4 cut(s) 212, 331, 480, 481
BseMII CTCAG 3 cut(s) 108, 242, 365
BseNI ACTGG 2 cut(s) 568, 810
BseSI GKGCMC 2 cut(s) 158, 693
BseXI GCAGC 2 cut(s) 70, 800
BseYI CCCAGC 2 cut(s) 331, 768
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 3 cut(s) 220, 418, 559
BsiHKAI GWGCWC 1 cut(s) 158
BsiSI CCGG 1 cut(s) 752
BslI CCNNNNNNNGG 4 cut(s) 212, 331, 480, 481
BsmAI GTCTC 1 cut(s) 665
BsmI GAATGC 2 cut(s) 73, 770
BsnI GGCC 3 cut(s) 220, 418, 559
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 158, 693, 876
Bsp143I GATC 5 cut(s) 41, 208, 508, 682, 847
BspANI GGCC 3 cut(s) 220, 418, 559
BspCNI CTCAG 3 cut(s) 109, 241, 366
BspLI GGNNCC 2 cut(s) 234, 718
BspPI GGATC 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 665
BsrI ACTGG 2 cut(s) 568, 810
BssECI CCNNGG 3 cut(s) 184, 236, 479
BssMI GATC 5 cut(s) 41, 208, 508, 682, 847
BssSI CACGAG 1 cut(s) 700
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 700
Bst2UI CCWGG 3 cut(s) 39, 222, 298
Bst4CI ACNGT 6 cut(s) 64, 240, 424, 449, 480, 826
Bst6I CTCTTC 2 cut(s) 165, 668
BstC8I GCNNGC 1 cut(s) 876
BstDEI CTNAG 3 cut(s) 117, 228, 374
BstDSI CCRYGG 2 cut(s) 236, 479
BstENI CCTNNNNNAGG 1 cut(s) 210
BstF5I GGATG 2 cut(s) 615, 814
BstKTI GATC 5 cut(s) 44, 211, 511, 685, 850
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 5 cut(s) 41, 208, 508, 682, 847
BstMWI GCNNNNNNNGC 2 cut(s) 97, 747
BstNI CCWGG 3 cut(s) 39, 222, 298
BstSCI CCNGG 4 cut(s) 37, 220, 296, 750
BstSLI GKGCMC 2 cut(s) 158, 693
BstV1I GCAGC 2 cut(s) 70, 800
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 220, 418, 559
BtgI CCRYGG 2 cut(s) 236, 479
BtsCI GGATG 2 cut(s) 615, 814
BtsIMutI CAGTG 1 cut(s) 822
Cac8I GCNNGC 1 cut(s) 876
Cfr13I GGNCC 1 cut(s) 416
Csp6I GTAC 2 cut(s) 48, 705
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 2 cut(s) 491, 865
CviJI RGCY 9 cut(s) 91, 192, 220, 331, 378, 418, 559, 741, 874
CviKI_1 RGCY 9 cut(s) 91, 192, 220, 331, 378, 418, 559, 741, 874
CviQI GTAC 2 cut(s) 48, 705
DdeI CTNAG 3 cut(s) 117, 228, 374
DpnI GATC 5 cut(s) 43, 210, 510, 684, 849
DpnII GATC 5 cut(s) 41, 208, 508, 682, 847
Eam1104I CTCTTC 2 cut(s) 165, 668
EarI CTCTTC 2 cut(s) 165, 668
Eco130I CCWWGG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 533
EcoNI CCTNNNNNAGG 1 cut(s) 210
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 3 cut(s) 37, 220, 296
EcoT14I CCWWGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 2 cut(s) 494, 868
FatI CATG 2 cut(s) 490, 864
FauNDI CATATG 1 cut(s) 855
FbaI TGATCA 2 cut(s) 208, 682
Fnu4HI GCNGC 2 cut(s) 84, 789
FokI GGATG 2 cut(s) 602, 821
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 2 cut(s) 84, 789
GluI GCNGC 2 cut(s) 84, 789
GsaI CCCAGC 2 cut(s) 335, 772
GsuI CTGGAG 1 cut(s) 585
HaeIII GGCC 3 cut(s) 220, 418, 559
HapII CCGG 1 cut(s) 752
Hin1II CATG 2 cut(s) 494, 868
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 2 cut(s) 199, 649
HpaII CCGG 1 cut(s) 752
HphI GGTGA 4 cut(s) 314, 506, 518, 658
Hpy166II GTNNAC 2 cut(s) 156, 705
Hpy188I TCNGA 5 cut(s) 146, 375, 513, 540, 610
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 2 cut(s) 156, 705
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 101, 177, 210, 535
HpyCH4III ACNGT 6 cut(s) 64, 240, 424, 449, 480, 826
HpyCH4V TGCA 4 cut(s) 71, 86, 156, 247
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 747
HpyF3I CTNAG 3 cut(s) 117, 228, 374
Hsp92II CATG 2 cut(s) 494, 868
Ksp22I TGATCA 2 cut(s) 208, 682
Kzo9I GATC 5 cut(s) 41, 208, 508, 682, 847
LmnI GCTCC 1 cut(s) 566
Lsp1109I GCAGC 2 cut(s) 70, 800
LweI GCATC 2 cut(s) 554, 624
MalI GATC 5 cut(s) 43, 210, 510, 684, 849
MboI GATC 5 cut(s) 41, 208, 508, 682, 847
MboII GAAGA 7 cut(s) 149, 152, 155, 308, 655, 809, 857
MfeI CAATTG 2 cut(s) 248, 530
MhlI GDGCHC 3 cut(s) 158, 693, 876
MluCI AATT 6 cut(s) 248, 262, 312, 530, 598, 880
Mph1103I ATGCAT 1 cut(s) 73
MseI TTAA 5 cut(s) 78, 261, 281, 366, 840
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 1 cut(s) 752
MspR9I CCNGG 4 cut(s) 39, 222, 298, 752
MunI CAATTG 2 cut(s) 248, 530
Mva1269I GAATGC 2 cut(s) 73, 770
MvaI CCWGG 3 cut(s) 39, 222, 298
MwoI GCNNNNNNNGC 2 cut(s) 97, 747
NciI CCSGG 1 cut(s) 752
NdeI CATATG 1 cut(s) 855
NdeII GATC 5 cut(s) 41, 208, 508, 682, 847
NlaIII CATG 2 cut(s) 494, 868
NlaIV GGNNCC 2 cut(s) 234, 718
NsiI ATGCAT 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 389
PceI AGGCCT 1 cut(s) 559
PctI GAATGC 2 cut(s) 73, 770
PfeI GAWTC 2 cut(s) 199, 649
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 2 cut(s) 85, 790
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 37, 220, 296
PspFI CCCAGC 2 cut(s) 331, 768
PspGI CCWGG 3 cut(s) 37, 220, 296
PspN4I GGNNCC 2 cut(s) 234, 718
PspPI GGNCC 1 cut(s) 416
RsaI GTAC 2 cut(s) 49, 706
RsaNI GTAC 2 cut(s) 48, 705
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 5 cut(s) 78, 261, 281, 366, 840
SatI GCNGC 2 cut(s) 84, 789
Sau3AI GATC 5 cut(s) 41, 208, 508, 682, 847
Sau96I GGNCC 1 cut(s) 416
ScrFI CCNGG 4 cut(s) 39, 222, 298, 752
SduI GDGCHC 3 cut(s) 158, 693, 876
SfaNI GCATC 2 cut(s) 554, 624
SmiMI CAYNNNNRTG 1 cut(s) 389
Sse9I AATT 6 cut(s) 248, 262, 312, 530, 598, 880
SseBI AGGCCT 1 cut(s) 559
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 4 cut(s) 37, 220, 296, 750
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 6 cut(s) 64, 240, 424, 449, 480, 826
TasI AATT 6 cut(s) 248, 262, 312, 530, 598, 880
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 2 cut(s) 199, 649
Tru1I TTAA 5 cut(s) 78, 261, 281, 366, 840
Tru9I TTAA 5 cut(s) 78, 261, 281, 366, 840
TscAI CASTG 1 cut(s) 829
TseI GCWGC 2 cut(s) 83, 788
TspDTI ATGAA 2 cut(s) 418, 810
TspRI CASTG 1 cut(s) 829
Van91I CCANNNNNTGG 1 cut(s) 331
VneI GTGCAC 1 cut(s) 154
VspI ATTAAT 1 cut(s) 261
XagI CCTNNNNNAGG 1 cut(s) 210
XapI RAATTY 3 cut(s) 312, 598, 880
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.