MD14G1089900.v1.1

Small acidic protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003825
Physical Location & Seq
Reverse (-)
11385812 .. 11391628
5817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1089900.v1.1.491

Sequence Viewer

Length: 1632 bp
ATGTGGGATCATAACACCCGAACGGATATAAAGCCCAAATCGGACATAAAACCCCATTCGAGGAGCCGAAAACCAAGGAACCGTCGTCCACCCAGAATTCGAAACAGAGACAGTTCCGATAGCAGAGCAGCCGTGACCCGCAAGCACAACATAAACGTGGTATTCATCATGGATTCAAACTTAGATTTGGGTGATGCAAATACAGCATTTCGCAAACCTTCAACTGATACAGCCAACCGGAAGTATCGCCGCCGTTCTCCGCTTAGCAGCTCATCTCCCTCTAATGGGAGTCCTGTGCATGAGCATAGTTCTAGCCCAAAAATTTCACAGGAAGATCCTGTAAAAGTCTCTGAATATCGAACAAGAAGGAATGAAGATGAAAGGAAATTTGAGAGGGATTCAAACAGGAGCTGTTATGGAAAAAGCAGTGATTCCTACAGGAATTCTGATACACAATCGTCCAAAACTTCACATGGTTACCATAGGCATGATGACTACATCAAACATGACAAACATGGTGATGAAGAAGAAAGAAATTATCAGAAGATAACTTCTCGTCTTGGTAGGGAGACAAGGGGTAGTGCTCATTCTGATCATACTAGGTCAAAAGACTATTTGCGTCCTTCGGACAAATCTTCTCGTGATAAATATGATGGTTCAGTATATAGGACCAAGGATAAAGATAGAGAATCTTCGTTTCCTCACCAGAAATATAAAGATAAGGACTCATCATCTGAGAAAGTTGGATATGGCAGGAGACATGGATATTTTGAAGAGATGGACAAGGAACGGGGTAGGCATACATTGGACAGAAAAGCTCCGGATGAAAACAGGGATTACCGTAGGAGTTCTGGAGATTATAGAAGTGAACGGATTTTCTCCTATGAAGAGTCCAAGGGGCAAAGAAGTGACTCAATTTCCAGGAGAGATGAAGGTAAACATTGTGTGAAAGAAGGTTACAAGAGTGAATTAAAGGAAATGGATGATGAAAACCATTCCAGAGAACAAAGGAAGAGATATGATGAGAGGGATAATAAAGTGAGGAGCCGAAATACTAGAGAACCAACAGAGCGGTCTGCAGATGATATTTCAAGGAGTGAAAATCAAGAATCTACTGCAAAAAGACCAAAGTTATTCAGCTCCGAGAAGAACACTGATGGCCGAAAAGATGTTTCTAAGTTCACAACTACAGCTGATGGAAAGGGGACTTCAAATTCTAAACCAAGTCAAGAAGGTATTGGGAAAACCATGTCCAGTGATGCAGAAGCTGCTAATGATATAAATGTCGCCAAATCTGCTGCAATGAAAGCTGCTGAAGTAGTTAATAGGAACCTTGTCAGTGTTGGTCCTGCGGGATGCATGACCGCTGACCAGAAGAAAAAGCTGTTGTGGGGAAACAAAAAGAACACGACTGCAGAAGAGGCTGGTCACCGTTGGGATACAGCATTGTTTTCCGACCGGGAAAGACAAGAAAAGTTCAACAAACTCATGGGTGTGAAGAGCGAAGTAAAGGTGGAAAACAAACCCGAAACCGAAGATCAGAAGCAGAAGGAACTCCAACTGGATTTGGAGAAGCAGTACACTGCTGGATTGCGAAGGAGAGATGGCCGTACTGTTGGATTAGGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMAP

Protein Analysis

544

Amino Acids

62.75

Weight (kDa)

9.63

Isoelectric Point (pI)

58.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMAP PF15477 479 - 542 7.4e-16 Small acidic protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1072
AccIII TCCGGA 1 cut(s) 820
AciI CCGC 6 cut(s) 139, 250, 260, 1072, 1352, 1365
AclWI GGATC 2 cut(s) 15, 329
AcoI YGGCCR 2 cut(s) 1159, 1606
AcsI RAATTY 5 cut(s) 96, 321, 386, 442, 1213
AcuI CTGAAG 1 cut(s) 1335
AfaI GTAC 2 cut(s) 1580, 1612
AfiI CCNNNNNNNGG 3 cut(s) 60, 284, 285
AgsI TTSAA 7 cut(s) 177, 222, 402, 773, 1092, 1212, 1480
AjnI CCWGG 1 cut(s) 920
AluBI AGCT 8 cut(s) 270, 411, 818, 1140, 1193, 1268, 1310, 1384
AluI AGCT 8 cut(s) 270, 411, 818, 1140, 1193, 1268, 1310, 1384
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 4 cut(s) 102, 352, 563, 751
AlwI GGATC 2 cut(s) 15, 329
AlwNI CAGNNNCTG 2 cut(s) 411, 1268
Aor13HI TCCGGA 1 cut(s) 820
AoxI GGCC 2 cut(s) 1159, 1606
ApeKI GCWGC 5 cut(s) 128, 267, 1268, 1298, 1310
ApoI RAATTY 5 cut(s) 96, 321, 386, 442, 1213
AspS9I GGNCC 2 cut(s) 669, 1346
AsuC2I CCSGG 1 cut(s) 1460
AsuHPI GGTGA 4 cut(s) 203, 530, 695, 1421
AsuII TTCGAA 1 cut(s) 100
AvaII GGWCC 2 cut(s) 669, 1346
BauI CACGAG 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 5 cut(s) 140, 279, 1255, 1285, 1297
BccI CCATC 5 cut(s) 647, 772, 1151, 1190, 1598
BceAI ACGGC 3 cut(s) 116, 237, 1593
BciT130I CCWGG 1 cut(s) 922
BciVI GTATCC 1 cut(s) 1432
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 1460
BcoDI GTCTC 4 cut(s) 102, 352, 563, 751
BfaI CTAG 3 cut(s) 312, 600, 1056
BfmI CTRYAG 4 cut(s) 436, 1077, 1188, 1413
BfuI GTATCC 1 cut(s) 1432
BisI GCNGC 6 cut(s) 129, 250, 268, 1269, 1299, 1311
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 6 cut(s) 130, 251, 269, 1270, 1300, 1312
Bme1390I CCNGG 2 cut(s) 922, 1460
Bme18I GGWCC 2 cut(s) 669, 1346
BmgT120I GGNCC 2 cut(s) 669, 1346
BmiI GGNNCC 4 cut(s) 65, 80, 1046, 1331
BmrFI CCNGG 2 cut(s) 922, 1460
BmsI GCATC 3 cut(s) 184, 1249, 1346
BpmI CTGGAG 1 cut(s) 873
Bpu1102I GCTNAGC 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 100
BpuMI CCSGG 1 cut(s) 1460
BsaJI CCNNGG 3 cut(s) 74, 672, 894
BsaWI WCCGGW 2 cut(s) 237, 820
BsaXI ACNNNNNCTCC 2 cut(s) 1562, 1592
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 284, 285
Bse1I ACTGG 2 cut(s) 1254, 1566
Bse3DI GCAATG 1 cut(s) 1308
BseAI TCCGGA 1 cut(s) 820
BseBI CCWGG 1 cut(s) 922
BseDI CCNNGG 3 cut(s) 74, 672, 894
BseGI GGATG 3 cut(s) 829, 988, 1361
BseLI CCNNNNNNNGG 3 cut(s) 60, 284, 285
BseMI GCAATG 1 cut(s) 1308
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 2 cut(s) 1254, 1566
BseRI GAGGAG 2 cut(s) 76, 1057
BseXI GCAGC 5 cut(s) 140, 279, 1255, 1285, 1297
Bsh1285I CGRYCG 1 cut(s) 1459
BshFI GGCC 2 cut(s) 1161, 1608
BsiEI CGRYCG 1 cut(s) 1459
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 3 cut(s) 238, 821, 1459
BslFI GGGAC 1 cut(s) 1219
BslI CCNNNNNNNGG 3 cut(s) 60, 284, 285
BsmAI GTCTC 4 cut(s) 102, 352, 563, 751
BsmFI GGGAC 1 cut(s) 1219
BsnI GGCC 2 cut(s) 1161, 1608
Bsp119I TTCGAA 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 586
Bsp13I TCCGGA 1 cut(s) 820
Bsp143I GATC 4 cut(s) 7, 334, 592, 1537
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 6 cut(s) 139, 250, 260, 1072, 1352, 1365
BspANI GGCC 2 cut(s) 1161, 1608
BspCNI CTCAG 1 cut(s) 727
BspEI TCCGGA 1 cut(s) 820
BspLI GGNNCC 4 cut(s) 65, 80, 1046, 1331
BspMAI CTGCAG 2 cut(s) 1081, 1417
BspPI GGATC 2 cut(s) 15, 329
BspQI GCTCTTC 1 cut(s) 1493
BspT104I TTCGAA 1 cut(s) 100
BsrBI CCGCTC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 1308
BsrI ACTGG 2 cut(s) 1254, 1566
BssECI CCNNGG 3 cut(s) 74, 672, 894
BssMI GATC 4 cut(s) 7, 334, 592, 1537
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 3 cut(s) 74, 672, 894
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 1 cut(s) 922
Bst4CI ACNGT 5 cut(s) 83, 113, 842, 1433, 1615
Bst6I CTCTTC 5 cut(s) 768, 882, 1007, 1413, 1493
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 4 cut(s) 181, 263, 735, 1176
BstEII GGTNACC 2 cut(s) 476, 1427
BstF5I GGATG 3 cut(s) 829, 988, 1361
BstKTI GATC 4 cut(s) 10, 337, 595, 1540
BstMAI GTCTC 4 cut(s) 102, 352, 563, 751
BstMBI GATC 4 cut(s) 7, 334, 592, 1537
BstMCI CGRYCG 1 cut(s) 1459
BstMWI GCNNNNNNNGC 5 cut(s) 203, 1268, 1295, 1307, 1421
BstNI CCWGG 1 cut(s) 922
BstPI GGTNACC 2 cut(s) 476, 1427
BstSCI CCNGG 2 cut(s) 920, 1458
BstSFI CTRYAG 4 cut(s) 436, 1077, 1188, 1413
BstV1I GCAGC 5 cut(s) 140, 279, 1255, 1285, 1297
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuI GTATCC 1 cut(s) 1432
BsuRI GGCC 2 cut(s) 1161, 1608
BtsCI GGATG 3 cut(s) 829, 988, 1361
BtsI GCAGTG 2 cut(s) 433, 1581
BtsIMutI CAGTG 5 cut(s) 433, 1152, 1261, 1345, 1581
Cac8I GCNNGC 1 cut(s) 143
CaiI CAGNNNCTG 2 cut(s) 411, 1268
Cfr13I GGNCC 2 cut(s) 669, 1346
CseI GACGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 1579, 1611
CviQI GTAC 2 cut(s) 1579, 1611
DdeI CTNAG 4 cut(s) 181, 263, 735, 1176
DpnI GATC 4 cut(s) 9, 336, 594, 1539
DpnII GATC 4 cut(s) 7, 334, 592, 1537
EaeI YGGCCR 2 cut(s) 1159, 1606
Eam1104I CTCTTC 5 cut(s) 768, 882, 1007, 1413, 1493
EarI CTCTTC 5 cut(s) 768, 882, 1007, 1413, 1493
Eco130I CCWWGG 3 cut(s) 74, 672, 894
Eco47I GGWCC 2 cut(s) 669, 1346
Eco57I CTGAAG 1 cut(s) 1335
Eco91I GGTNACC 2 cut(s) 476, 1427
EcoO65I GGTNACC 2 cut(s) 476, 1427
EcoRI GAATTC 2 cut(s) 96, 442
EcoRII CCWGG 1 cut(s) 920
EcoT14I CCWWGG 3 cut(s) 74, 672, 894
EcoT22I ATGCAT 1 cut(s) 1361
ErhI CCWWGG 3 cut(s) 74, 672, 894
FaqI GGGAC 1 cut(s) 1219
FauI CCCGC 2 cut(s) 146, 1345
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 129, 250, 268, 1269, 1299, 1311
FokI GGATG 3 cut(s) 836, 995, 1368
Fsp4HI GCNGC 6 cut(s) 129, 250, 268, 1269, 1299, 1311
FspBI CTAG 3 cut(s) 312, 600, 1056
GluI GCNGC 6 cut(s) 129, 250, 268, 1269, 1299, 1311
GsuI CTGGAG 1 cut(s) 873
HaeIII GGCC 2 cut(s) 1161, 1608
HapII CCGG 3 cut(s) 238, 821, 1459
HgaI GACGC 1 cut(s) 608
HinfI GANTC 9 cut(s) 173, 289, 398, 431, 689, 725, 890, 911, 1109
HpaII CCGG 3 cut(s) 238, 821, 1459
HphI GGTGA 4 cut(s) 203, 530, 695, 1421
Hpy166II GTNNAC 5 cut(s) 89, 869, 938, 1182, 1581
Hpy188III TCNNGA 6 cut(s) 641, 821, 852, 999, 1106, 1229
Hpy8I GTNNAC 5 cut(s) 89, 869, 938, 1182, 1581
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 8 cut(s) 228, 360, 633, 926, 947, 1226, 1543, 1590
HpyCH4III ACNGT 5 cut(s) 83, 113, 842, 1433, 1615
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 8 cut(s) 197, 298, 1079, 1118, 1262, 1301, 1359, 1415
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 1268, 1295, 1307, 1421
HpyF3I CTNAG 4 cut(s) 181, 263, 735, 1176
HpySE526I ACGT 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 820
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 7, 334, 592, 1537
LguI GCTCTTC 1 cut(s) 1493
LmnI GCTCC 5 cut(s) 63, 408, 823, 1044, 1145
Lsp1109I GCAGC 5 cut(s) 140, 279, 1255, 1285, 1297
LweI GCATC 3 cut(s) 184, 1249, 1346
MaeI CTAG 3 cut(s) 312, 600, 1056
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 5 cut(s) 133, 476, 908, 956, 1427
MalI GATC 4 cut(s) 9, 336, 594, 1539
MbiI CCGCTC 1 cut(s) 1072
MboI GATC 4 cut(s) 7, 334, 592, 1537
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 8 cut(s) 96, 321, 386, 442, 535, 915, 968, 1213
MlyI GAGTC 4 cut(s) 298, 719, 899, 905
MmeI TCCRAC 4 cut(s) 724, 1479, 1582, 1597
MnlI CCTC 7 cut(s) 54, 289, 387, 711, 1020, 1035, 1414
Mph1103I ATGCAT 1 cut(s) 1361
MroI TCCGGA 1 cut(s) 820
MseI TTAA 3 cut(s) 971, 1323, 1630
MslI CAYNNNNRTG 4 cut(s) 155, 486, 519, 1493
MspA1I CMGCKG 2 cut(s) 1193, 1367
MspI CCGG 3 cut(s) 238, 821, 1459
MspR9I CCNGG 2 cut(s) 922, 1460
MvaI CCWGG 1 cut(s) 922
MwoI GCNNNNNNNGC 5 cut(s) 203, 1268, 1295, 1307, 1421
NciI CCSGG 1 cut(s) 1460
NdeII GATC 4 cut(s) 7, 334, 592, 1537
NlaIV GGNNCC 4 cut(s) 65, 80, 1046, 1331
NmuCI GTSAC 3 cut(s) 133, 908, 1427
NsiI ATGCAT 1 cut(s) 1361
NspV TTCGAA 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 1493
PfeI GAWTC 5 cut(s) 173, 398, 431, 689, 1109
PfoI TCCNGGA 1 cut(s) 920
PkrI GCNGC 6 cut(s) 130, 251, 269, 1270, 1300, 1312
PleI GAGTC 4 cut(s) 297, 719, 898, 905
PpsI GAGTC 4 cut(s) 297, 719, 898, 905
Psp6I CCWGG 1 cut(s) 920
PspEI GGTNACC 2 cut(s) 476, 1427
PspGI CCWGG 1 cut(s) 920
PspN4I GGNNCC 4 cut(s) 65, 80, 1046, 1331
PspPI GGNCC 2 cut(s) 669, 1346
PstI CTGCAG 2 cut(s) 1081, 1417
PstNI CAGNNNCTG 2 cut(s) 411, 1268
PsuI RGATCY 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 2 cut(s) 1580, 1612
RsaNI GTAC 2 cut(s) 1579, 1611
RseI CAYNNNNRTG 4 cut(s) 155, 486, 519, 1493
SapI GCTCTTC 1 cut(s) 1493
SaqAI TTAA 3 cut(s) 971, 1323, 1630
SatI GCNGC 6 cut(s) 129, 250, 268, 1269, 1299, 1311
Sau3AI GATC 4 cut(s) 7, 334, 592, 1537
Sau96I GGNCC 2 cut(s) 669, 1346
SchI GAGTC 4 cut(s) 298, 719, 899, 905
ScrFI CCNGG 2 cut(s) 922, 1460
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 3 cut(s) 184, 1249, 1346
SfcI CTRYAG 4 cut(s) 436, 1077, 1188, 1413
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 2 cut(s) 669, 1346
SmiMI CAYNNNNRTG 4 cut(s) 155, 486, 519, 1493
Sse9I AATT 8 cut(s) 96, 321, 386, 442, 535, 915, 968, 1213
SsiI CCGC 6 cut(s) 139, 250, 260, 1072, 1352, 1365
SspMI CTAG 3 cut(s) 312, 600, 1056
StyD4I CCNGG 2 cut(s) 920, 1458
StyI CCWWGG 3 cut(s) 74, 672, 894
TaaI ACNGT 5 cut(s) 83, 113, 842, 1433, 1615
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 59, 100, 358
TasI AATT 8 cut(s) 96, 321, 386, 442, 535, 915, 968, 1213
TatI WGTACW 1 cut(s) 1578
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 5 cut(s) 173, 398, 431, 689, 1109
Tru1I TTAA 3 cut(s) 971, 1323, 1630
Tru9I TTAA 3 cut(s) 971, 1323, 1630
TscAI CASTG 5 cut(s) 433, 1159, 1261, 1345, 1588
TseFI GTSAC 3 cut(s) 133, 908, 1427
TseI GCWGC 5 cut(s) 128, 267, 1268, 1298, 1310
Tsp45I GTSAC 3 cut(s) 133, 908, 1427
TspDTI ATGAA 9 cut(s) 154, 387, 393, 537, 840, 900, 945, 1002, 1319
TspGWI ACGGA 2 cut(s) 38, 886
TspRI CASTG 5 cut(s) 433, 1159, 1261, 1345, 1588
VpaK11BI GGWCC 2 cut(s) 669, 1346
XapI RAATTY 5 cut(s) 96, 321, 386, 442, 1213
XspI CTAG 3 cut(s) 312, 600, 1056
Zsp2I ATGCAT 1 cut(s) 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.