Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003825

Physical Location & Seq

Genomic Coordinates Reverse (-)

13163430 .. 13166794

Gene Structure 3365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0098500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3309 bp

ATGTGGGTACCTCATCCTGAGTGTTCAAAGGTGGTGGAACAAGGATGGATGCTTCCCACCTCAGGGGACTCGATGTTACAGGTTGGACGAAAGATCCAACATACAGGGCAACTTTTTATGAGTTGGAATTCTAGGGTTTTCCAACAGCGACAAACTGAAATGAAATTGATAGAAGGTAAACTGGGGGAGTTGATGAAGCGTGGTTTTGAAGATGGACATTTTGACGAACAGAAAGCGTTACAATTTCGCTTGAATGAATTGCTCACTGCTAATGAAACTTATTGGAGACAAAGATCCAGAGTTCAATGGTTGAAAGAAGGTGACCGTAATACTGCTTTTTTTCATAGAAAAGCTTCGAATCGTAGATGTCGAAATCGGATCAAAGGACTTTCTAACGAAGCAGGCCAATGGTGTTCTAAACCTGAGGAAATCGCAGACATTTTGATACGGTACTATGAGGAGATCTTTCAAGCTGGCAGGTCGGATCCTATAGCCCTTAACACGATCCTAAATTGTTTGCAACCTAGGGTTTCTGCGGAAATGAACGCAGACCTTACTGCCCCTTATTCTGATGGTGAGATTAAGCGGCCACTATTTCAAATGCAGCCTTCTAAATCGCCGGGTCCTGATGGCATGTCTCCTTGTTTCTTTCAGAAATTCTGGAATGTGGTTGAAAGGGATGTTTGTATGGCAGTTCGTGAGGTGTTGGGTACAGGTCAGATTGCTCAAGAGTCCAATTTTACTCACCTTACTCTCATTCCAAAGATTAAGGATCCCAAACTCCCTTCAAACTTAAGGCCTATAGCATTATGTAATGTGGTGTATAAGATTGCCTCCAAGGTTCTGGCTAACAGACTCAAGGCGATCCTCCCTCATGTTATCTCTCCTCTGCAAAGCGTGTTTGTTCCAGGTAGACTCATATCGGATAATACTTTGGTGGCAACCGAAGCTGAGGGTTTCTTTTCTTTAAAACTCGACATCTCCAAAGCATATGATCGACTTGAATGGACGTATTTAGAGGCTATTTTGCTCAAATTGAGGTTTTGTCGAAGTTGGGTGGAAGTTGTCCTCTCCACAGTCAAGTCGGTTAGTTATTCTATTTTGTTCAATGGCTCACCTACGGGTTTTATCTTGCCTTCGAGGGGTATCCGACAAGGGGATCCCTTATCCCCATACTTGTTTATTCTGTGCGCAGAGGGACTTTCTGCACTTATTTCCTCTCGTGTTCAGAATGGTATCATTAAAGGTCTCACTATGACTCCTACAGCTCCTACTATTCATCATCTTTTATTTGCAGACGACAGTTTTTTGTTTGGAGAAGCTTCAAGCCGTGAATGTCAAGCTTTTAAACAGGTACTATCCATCTATGCAAGAGCTTCTGGTCAACATATTAACCTACAGAAATCTAGTGTTGTTTTTAGTGGTAATGTTAGCCAGCGAGTCAGGACTTCTTTAGCCTCTATACTCGGAGTGCAGTGTGTTGAAGAGCATGGCTTGTATTTGGGTTTGCCTATCCATGTTGGACATAACAAGAAGGACATTTTTGCCTACTTGAAAGAGAGGTTATCCAAGAAACTCATTAGTTGGCGGTCAAAAATATTGAGCTCAGGAGGTAAAGAATTACTCATTAAAGCTGTAGCCCAAACTTTGCCTAATTATGTGATGAATTGCTATGTTTTGCCAAAGTCCATGTGTGATGATTTACAGCAACTCTGTTGTCAATTTTTCTGGGGAAGTACAGATGATAAAAGAAAAATTCATTGGAGGTCTTGGGAGAGGATGTGTCTCCCTAAGGACAAAGGAGGTATGGGGTTTAAACACCTGCATTCGCACAACTTAGCCATGCTCTCGAAACAAGGCTGGAGGCTTCTTACCAACCCGGATTCTCTTGTGGCCCGAGTCTTCAAAGCAGTGTATTATCCGTTTGGCTCTTTCCTAACAGCTGATATGGGTGACCGGCCTTCATACTCATGGAGAAGTCTTATGGAGGCAAGATCGGTCTTGCAAGCAGGCTTGTTGTGGCGTATTGGCAATGGCCAATCAGTTAGAATTTGGGAAGATGAGTGGATCCCTAATGTCTCTCATTACTCTTTATCACGACCCTCGGACTCGGTTTTTGAGACAGTCTCTGACTTGATTAATCATGATTCATTGTCATGGGATATCCCTGCAGTTCACTCTTGCTTTGAACCTTCTGTTGTGGCACAAGTCTTGGCAATTCCTCTAAGTAGGCGCTTTGAAAGAGATAAGCCTGCATGGAAGCTCGATAAGAAAGGTTACTTCTCGGTCAAATCTGCATACAAAATTGCATGTGACTTCTCTCTCGGTAATTTATGTGCCTCAGCCTCCACGGGGGATCCATATGGTCCTATATGGAAAGCTTTGTGGAAAGCACAGGTTCCTAGCAAAGTTGCCATATTTGGCTGGAGAGCTATTCACAATCTCCTTCCCACACGATCTGCTTTATCCTTGAAGGGTTACACGGGTACAATGCAGTGTGTTATGTGCTCTACCCATGTGGAAACTTTAGAGCACTTACTCTGTCATTGTAGCTTTGCGCGTGAGATCCTTGGTGCACCGCCTTTCTCTATTCCCATTTCATCCTTGTGCTGGAAAGATTGGATCTTGGATAGAGTCTTGTCACTATCTTCAGGGGCCTTTGACCAGCTACTAGTGATGCTTTGGAGTATTTGGAAAAACCGTAACGATAAGCTATGGCATGATCATGTTCAACCGAGTGCTACTCTGGTGGCCAAGGCTATGGCTTGGCATGAGGAATATCTACAGGCTAACTGCTCTACTAGTACTCATTCTCATCGTCGTCCTAAGCCCCGTAAAGAATGGGTTGCCCCAGATGGTGAGTGTTTGAAACTTAATGTGGATGGAACGTTTCTACCTTCGATTCCGTATGGTGGTACGGGAGGTGTGATCAGGAACTCTCATGGACAGTTTATTGCTGGCTTCTCATTCCGAATGGATTTTGTTAGCTCACCCTTACATGCTGAGTTGCTTGCCTTGAAGAATGGGCTGGAATTGTTGCAGGCTATGAGTATTACTCGTGCTATACTATTGAAAACTATCAATTCCTTAACCCCAGACCTCTCTCCTTTGGGTGCATTGATCGTGGATATTCAAGGACTGCTAGCTGCCAACATGGACGTCCAACTCTCACATGTTCCTCTTCAGGCTAACACTGTGGCACATAGGTTGGCTAATTATAGTTTTGAATGTAATGTTCATCTTGAATGGTTCTCTATTGCTCCAGAGTTCATCCTGGATGCTCTTTTGTACTATTTCAATCGTATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SMAP

Protein Analysis

1102

Amino Acids

124.4

Weight (kDa)

9.07

Isoelectric Point (pI)

40.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_1	PF00078	259 - 494	3.5e-27	Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT	PF13966	761 - 856	2.8e-17	zinc-binding in reverse transcriptase
RVT_3	PF13456	959 - 1073	1e-14	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003825)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56720 AT3G56720 AT3G56720 AT3G56720 AT3G56720 AT3G56720 AT3G56720
fragaria_vesca	FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070 FvH4_2g14070
malus_domestica	MD14G1089900.v1.1
prunus_persica	Prupe.7G034100_v2.0.a1
pyrus_communis	pycom12g08390
rosa_chinensis	RchiOBHm_Chr6g0276151
rosa_laevigata	RLG00000013413
rosa_multiflora	Rmu_ssc0000201.1_g000001
rosa_roxburghii	Rroxscaffold_7G00192520
rosa_rugosa	Rorug06G0098300 Rorug06G0098400 Rorug06G0098500
rosa_samantha	Rh6AG210300 Rh6BG214400 Rh6BG214500 Rh6CG217200 Rh6DG206500 Rh6DG206700
rosa_wichuraiana	Rw6G018320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1830
AatII	GACGTC	1 cut(s)	3162
Acc16I	TGCGCA	1 cut(s)	1192
Acc36I	ACCTGC	2 cut(s)	468, 1830
Acc65I	GGTACC	1 cut(s)	7
AccB1I	GGYRCC	1 cut(s)	7
AccI	GTMKAC	1 cut(s)	913
AccII	CGCG	1 cut(s)	2560
AciI	CCGC	4 cut(s)	536, 586, 1588, 2579
AclI	AACGTT	1 cut(s)	2888
AcoI	YGGCCR	3 cut(s)	587, 2035, 2751
AcsI	RAATTY	4 cut(s)	127, 656, 1755, 2049
AcuI	CTGAAG	2 cut(s)	2634, 3167
AcyI	GRCGYC	1 cut(s)	3159
AfaI	GTAC	9 cut(s)	9, 452, 712, 1356, 1738, 2488, 2806, 2917, 3290
AfiI	CCNNNNNNNGG	7 cut(s)	62, 63, 1142, 1156, 2352, 2610, 2912
AflII	CTTAAG	1 cut(s)	793
AflIII	ACRYGT	1 cut(s)	3172
AhlI	ACTAGT	2 cut(s)	2671, 2801
AjnI	CCWGG	2 cut(s)	907, 3273
AjuI	GAANNNNNNNTTGG	2 cut(s)	1744, 1776
Alw21I	GWGCWC	4 cut(s)	1607, 2510, 2535, 2578
Alw26I	GTCTC	7 cut(s)	280, 642, 1253, 1790, 2083, 2114, 2131
Alw44I	GTGCAC	1 cut(s)	2574
AlwNI	CAGNNNCTG	1 cut(s)	2129
Ama87I	CYCGRG	1 cut(s)	1896
AoxI	GGCC	8 cut(s)	403, 587, 797, 1893, 1958, 2035, 2655, 2751
ApaLI	GTGCAC	1 cut(s)	2574
ApeKI	GCWGC	2 cut(s)	604, 3146
ApoI	RAATTY	4 cut(s)	127, 656, 1755, 2049
ArsI	GACNNNNNNTTYG	2 cut(s)	2099, 2131
AseI	ATTAAT	1 cut(s)	2139
Asp700I	GAANNNNTTC	1 cut(s)	352
Asp718I	GGTACC	1 cut(s)	7
AspA2I	CCTAGG	1 cut(s)	524
AspLEI	GCGC	3 cut(s)	1193, 2235, 2560
AspS9I	GGNCC	4 cut(s)	623, 1894, 2366, 2655
AsuC2I	CCSGG	2 cut(s)	621, 1880
AsuHPI	GGTGA	7 cut(s)	332, 587, 737, 1107, 1964, 2870, 2982
AsuII	TTCGAA	1 cut(s)	356
AsuNHI	GCTAGC	1 cut(s)	3142
AvaI	CYCGRG	1 cut(s)	1896
AvaII	GGWCC	2 cut(s)	623, 2366
AvrII	CCTAGG	1 cut(s)	524
AxyI	CCTNAGG	3 cut(s)	61, 423, 1791
BaeGI	GKGCMC	1 cut(s)	2578
BalI	TGGCCA	2 cut(s)	2037, 2753
BamHI	GGATCC	5 cut(s)	484, 772, 1159, 2067, 2356
BanI	GGYRCC	1 cut(s)	7
BanII	GRGCYC	1 cut(s)	1607
BauI	CACGAG	2 cut(s)	1221, 3057
BbsI	GAAGAC	1 cut(s)	1894
Bbv12I	GWGCWC	4 cut(s)	1607, 2510, 2535, 2578
BbvCI	CCTCAGC	2 cut(s)	951, 2341
BbvI	GCAGC	2 cut(s)	616, 3133
BccI	CCATC	7 cut(s)	39, 206, 566, 623, 1370, 2849, 2876
BceAI	ACGGC	1 cut(s)	1314
BciT130I	CCWGG	2 cut(s)	909, 3275
BciVI	GTATCC	1 cut(s)	1157
BclI	TGATCA	2 cut(s)	2722, 2928
BcnI	CCSGG	2 cut(s)	621, 1880
BcoDI	GTCTC	7 cut(s)	280, 642, 1253, 1790, 2083, 2114, 2131
BcuI	ACTAGT	2 cut(s)	2671, 2801
BfaI	CTAG	8 cut(s)	132, 525, 1407, 2403, 2672, 2802, 3143, 3307
BfmI	CTRYAG	7 cut(s)	489, 801, 1263, 1397, 1635, 2169, 2783
BfoI	RGCGCY	1 cut(s)	2236
BfrI	CTTAAG	1 cut(s)	793
BfuAI	ACCTGC	2 cut(s)	468, 1830
BfuI	GTATCC	1 cut(s)	1157
BglII	AGATCT	1 cut(s)	462
BisI	GCNGC	3 cut(s)	587, 605, 3147
BlnI	CCTAGG	1 cut(s)	524
BlsI	GCNGC	3 cut(s)	588, 606, 3148
BmcAI	AGTACT	1 cut(s)	2806
Bme1390I	CCNGG	4 cut(s)	621, 909, 1880, 3275
Bme18I	GGWCC	2 cut(s)	623, 2366
BmeT110I	CYCGRG	1 cut(s)	1896
BmgT120I	GGNCC	4 cut(s)	623, 1894, 2366, 2655
BmiI	GGNNCC	9 cut(s)	9, 486, 624, 774, 1161, 2069, 2358, 2400, 2656
BmrFI	CCNGG	4 cut(s)	621, 909, 1880, 3275
BmrI	ACTGGG	1 cut(s)	191
BmsI	GCATC	3 cut(s)	39, 2667, 3268
BmtI	GCTAGC	1 cut(s)	3146
BmuI	ACTGGG	1 cut(s)	191
BpiI	GAAGAC	1 cut(s)	1894
BplI	GAGNNNNNCTC	6 cut(s)	2111, 2143, 2728, 2760, 3040, 3072
BpmI	CTGGAG	3 cut(s)	1882, 2446, 3246
Bpu10I	CCTNAGC	4 cut(s)	951, 1606, 2341, 2826
Bpu14I	TTCGAA	1 cut(s)	356
BpuEI	CTTGAG	2 cut(s)	711, 842
BpuMI	CCSGG	2 cut(s)	621, 1880
BsaHI	GRCGYC	1 cut(s)	3159
BsaI	GGTCTC	1 cut(s)	1253
BsaJI	CCNNGG	6 cut(s)	524, 837, 2103, 2349, 2569, 2754
BsaXI	ACNNNNNCTCC	4 cut(s)	1243, 1273, 1766, 1796
Bsc4I	CCNNNNNNNGG	7 cut(s)	62, 63, 1142, 1156, 2352, 2610, 2912
Bse118I	RCCGGY	1 cut(s)	1956
Bse1I	ACTGG	1 cut(s)	186
Bse21I	CCTNAGG	3 cut(s)	61, 423, 1791
Bse3DI	GCAATG	1 cut(s)	2038
BseBI	CCWGG	2 cut(s)	909, 3275
BseDI	CCNNGG	6 cut(s)	524, 837, 2103, 2349, 2569, 2754
BseGI	GGATG	9 cut(s)	13, 50, 54, 685, 1785, 2600, 2887, 3270, 3283
BseLI	CCNNNNNNNGG	7 cut(s)	62, 63, 1142, 1156, 2352, 2610, 2912
BseMI	GCAATG	1 cut(s)	2038
BseMII	CTCAG	7 cut(s)	9, 75, 414, 942, 1620, 2355, 2994
BseNI	ACTGG	1 cut(s)	186
BseRI	GAGGAG	2 cut(s)	473, 876
BseSI	GKGCMC	1 cut(s)	2578
BseXI	GCAGC	2 cut(s)	616, 3133
BsgI	GTGCAG	2 cut(s)	1191, 1493
Bsh1236I	CGCG	1 cut(s)	2560
BshFI	GGCC	8 cut(s)	405, 589, 799, 1895, 1960, 2037, 2657, 2753
BshNI	GGYRCC	1 cut(s)	7
BsiHKAI	GWGCWC	4 cut(s)	1607, 2510, 2535, 2578
BsiHKCI	CYCGRG	1 cut(s)	1896
BsiSI	CCGG	3 cut(s)	620, 1880, 1957
BslFI	GGGAC	2 cut(s)	80, 1212
BslI	CCNNNNNNNGG	7 cut(s)	62, 63, 1142, 1156, 2352, 2610, 2912
BsmAI	GTCTC	7 cut(s)	280, 642, 1253, 1790, 2083, 2114, 2131
BsmFI	GGGAC	2 cut(s)	80, 1212
BsmI	GAATGC	1 cut(s)	1825
BsnI	GGCC	8 cut(s)	405, 589, 799, 1895, 1960, 2037, 2657, 2753
Bso31I	GGTCTC	1 cut(s)	1253
BsoBI	CYCGRG	1 cut(s)	1896
Bsp119I	TTCGAA	1 cut(s)	356
Bsp1286I	GDGCHC	4 cut(s)	1607, 2510, 2535, 2578
BspACI	CCGC	4 cut(s)	536, 586, 1588, 2579
BspANI	GGCC	8 cut(s)	405, 589, 799, 1895, 1960, 2037, 2657, 2753
BspCNI	CTCAG	7 cut(s)	10, 74, 415, 943, 1619, 2354, 2995
BspFNI	CGCG	1 cut(s)	2560
BspHI	TCATGA	1 cut(s)	2143
BspLI	GGNNCC	9 cut(s)	9, 486, 624, 774, 1161, 2069, 2358, 2400, 2656
BspMAI	CTGCAG	1 cut(s)	2173
BspMI	ACCTGC	2 cut(s)	468, 1830
BspOI	GCTAGC	1 cut(s)	3146
BspQI	GCTCTTC	1 cut(s)	1479
BspT104I	TTCGAA	1 cut(s)	356
BspT107I	GGYRCC	1 cut(s)	7
BspTI	CTTAAG	1 cut(s)	793
BspTNI	GGTCTC	1 cut(s)	1253
BsrDI	GCAATG	1 cut(s)	2038
BsrFI	RCCGGY	1 cut(s)	1956
BsrI	ACTGG	1 cut(s)	186
BssAI	RCCGGY	1 cut(s)	1956
BssECI	CCNNGG	6 cut(s)	524, 837, 2103, 2349, 2569, 2754
BssNI	GRCGYC	1 cut(s)	3159
BssSI	CACGAG	2 cut(s)	1221, 3057
BssT1I	CCWWGG	4 cut(s)	524, 837, 2569, 2754
Bst2BI	CACGAG	2 cut(s)	1221, 3057
Bst2UI	CCWGG	2 cut(s)	909, 3275
Bst4CI	ACNGT	8 cut(s)	326, 450, 1078, 1304, 2125, 2702, 2949, 3196
Bst6I	CTCTTC	2 cut(s)	1479, 3186
BstACI	GRCGYC	1 cut(s)	3159
BstAFI	CTTAAG	1 cut(s)	793
BstBI	TTCGAA	1 cut(s)	356
BstDSI	CCRYGG	1 cut(s)	2349
BstEII	GGTNACC	2 cut(s)	320, 1952
BstF5I	GGATG	9 cut(s)	13, 50, 54, 685, 1785, 2600, 2887, 3270, 3283
BstFNI	CGCG	1 cut(s)	2560
BstH2I	RGCGCY	1 cut(s)	2236
BstHHI	GCGC	3 cut(s)	1193, 2235, 2560
BstMAI	GTCTC	7 cut(s)	280, 642, 1253, 1790, 2083, 2114, 2131
BstMWI	GCNNNNNNNGC	1 cut(s)	947
BstNI	CCWGG	2 cut(s)	909, 3275
BstNSI	RCATGY	4 cut(s)	637, 2313, 3002, 3176
BstPI	GGTNACC	2 cut(s)	320, 1952
BstSCI	CCNGG	4 cut(s)	619, 907, 1878, 3273
BstSFI	CTRYAG	7 cut(s)	489, 801, 1263, 1397, 1635, 2169, 2783
BstSLI	GKGCMC	1 cut(s)	2578
BstUI	CGCG	1 cut(s)	2560
BstV1I	GCAGC	2 cut(s)	616, 3133
BstV2I	GAAGAC	1 cut(s)	1894
BstXI	CCANNNNNNTGG	2 cut(s)	844, 2761
Bsu36I	CCTNAGG	3 cut(s)	61, 423, 1791
BsuI	GTATCC	1 cut(s)	1157
BsuRI	GGCC	8 cut(s)	405, 589, 799, 1895, 1960, 2037, 2657, 2753
BtgI	CCRYGG	1 cut(s)	2349
BtsCI	GGATG	9 cut(s)	13, 50, 54, 685, 1785, 2600, 2887, 3270, 3283
BtsI	GCAGTG	4 cut(s)	264, 1481, 1917, 2501
BtsIMutI	CAGTG	5 cut(s)	264, 1481, 1917, 2501, 3192
BveI	ACCTGC	2 cut(s)	468, 1830
CaiI	CAGNNNCTG	1 cut(s)	2129
CciI	TCATGA	1 cut(s)	2143
CfoI	GCGC	3 cut(s)	1193, 2235, 2560
Cfr10I	RCCGGY	1 cut(s)	1956
Cfr13I	GGNCC	4 cut(s)	623, 1894, 2366, 2655
Csp6I	GTAC	9 cut(s)	8, 451, 711, 1355, 1737, 2487, 2805, 2916, 3289
CspCI	CAANNNNNGTGG	2 cut(s)	15, 50
CviQI	GTAC	9 cut(s)	8, 451, 711, 1355, 1737, 2487, 2805, 2916, 3289
DraI	TTTAAA	3 cut(s)	969, 1348, 1816
EaeI	YGGCCR	3 cut(s)	587, 2035, 2751
Eam1104I	CTCTTC	2 cut(s)	1479, 3186
EarI	CTCTTC	2 cut(s)	1479, 3186
Ecl136II	GAGCTC	1 cut(s)	1605
Eco130I	CCWWGG	4 cut(s)	524, 837, 2569, 2754
Eco147I	AGGCCT	1 cut(s)	799
Eco24I	GRGCYC	1 cut(s)	1607
Eco31I	GGTCTC	1 cut(s)	1253
Eco32I	GATATC	1 cut(s)	2164
Eco47I	GGWCC	2 cut(s)	623, 2366
Eco53kI	GAGCTC	1 cut(s)	1605
Eco57I	CTGAAG	2 cut(s)	2634, 3167
Eco81I	CCTNAGG	3 cut(s)	61, 423, 1791
Eco88I	CYCGRG	1 cut(s)	1896
Eco91I	GGTNACC	2 cut(s)	320, 1952
EcoICRI	GAGCTC	1 cut(s)	1605
EcoO109I	RGGNCCY	2 cut(s)	623, 2655
EcoO65I	GGTNACC	2 cut(s)	320, 1952
EcoRI	GAATTC	1 cut(s)	127
EcoRII	CCWGG	2 cut(s)	907, 3273
EcoRV	GATATC	1 cut(s)	2164
EcoT14I	CCWWGG	4 cut(s)	524, 837, 2569, 2754
EcoT38I	GRGCYC	1 cut(s)	1607
ErhI	CCWWGG	4 cut(s)	524, 837, 2569, 2754
FaqI	GGGAC	2 cut(s)	80, 1212
FauNDI	CATATG	2 cut(s)	991, 2362
FbaI	TGATCA	2 cut(s)	2722, 2928
FblI	GTMKAC	1 cut(s)	913
Fnu4HI	GCNGC	3 cut(s)	587, 605, 3147
FokI	GGATG	8 cut(s)	57, 61, 692, 1792, 2587, 2894, 3257, 3290
FriOI	GRGCYC	1 cut(s)	1607
Fsp4HI	GCNGC	3 cut(s)	587, 605, 3147
FspBI	CTAG	8 cut(s)	132, 525, 1407, 2403, 2672, 2802, 3143, 3307
FspI	TGCGCA	1 cut(s)	1192
GlaI	GCGC	3 cut(s)	1192, 2234, 2559
GluI	GCNGC	3 cut(s)	587, 605, 3147
GsuI	CTGGAG	3 cut(s)	1882, 2446, 3246
HaeII	RGCGCY	1 cut(s)	2236
HaeIII	GGCC	8 cut(s)	405, 589, 799, 1895, 1960, 2037, 2657, 2753
HapII	CCGG	3 cut(s)	620, 1880, 1957
HhaI	GCGC	3 cut(s)	1193, 2235, 2560
Hin1I	GRCGYC	1 cut(s)	3159
Hin6I	GCGC	3 cut(s)	1191, 2233, 2558
HinP1I	GCGC	3 cut(s)	1191, 2233, 2558
HincII	GTYRAC	1 cut(s)	1385
HindII	GTYRAC	1 cut(s)	1385
HindIII	AAGCTT	4 cut(s)	351, 1320, 1341, 2379
HpaII	CCGG	3 cut(s)	620, 1880, 1957
HphI	GGTGA	7 cut(s)	332, 587, 737, 1107, 1964, 2870, 2982
Hpy166II	GTNNAC	5 cut(s)	179, 914, 1385, 2176, 2576
Hpy8I	GTNNAC	5 cut(s)	179, 914, 1385, 2176, 2576
Hpy99I	CGWCG	1 cut(s)	2823
HpyCH4III	ACNGT	8 cut(s)	326, 450, 1078, 1304, 2125, 2702, 2949, 3196
HpyCH4IV	ACGT	3 cut(s)	1010, 2888, 3159
HpyF10VI	GCNNNNNNNGC	1 cut(s)	947
HpySE526I	ACGT	3 cut(s)	1010, 2888, 3159
Hsp92I	GRCGYC	1 cut(s)	3159
HspAI	GCGC	3 cut(s)	1191, 2233, 2558
KpnI	GGTACC	1 cut(s)	11
Ksp22I	TGATCA	2 cut(s)	2722, 2928
LguI	GCTCTTC	1 cut(s)	1479
LmnI	GCTCC	2 cut(s)	1273, 3265
Lsp1109I	GCAGC	2 cut(s)	616, 3133
LweI	GCATC	3 cut(s)	39, 2667, 3268
MaeI	CTAG	8 cut(s)	132, 525, 1407, 2403, 2672, 2802, 3143, 3307
MaeII	ACGT	3 cut(s)	1010, 2888, 3159
MaeIII	GTNAC	9 cut(s)	75, 237, 320, 1952, 2276, 2312, 2477, 2640, 2702
MboII	GAAGA	7 cut(s)	221, 1496, 1894, 2069, 2640, 3031, 3173
MhlI	GDGCHC	4 cut(s)	1607, 2510, 2535, 2578
MlsI	TGGCCA	2 cut(s)	2037, 2753
MluNI	TGGCCA	2 cut(s)	2037, 2753
MlyI	GAGTC	9 cut(s)	62, 740, 849, 909, 1252, 1449, 1908, 2102, 2643
MmeI	TCCRAC	8 cut(s)	64, 104, 121, 166, 462, 1174, 1501, 3187
Mox20I	TGGCCA	2 cut(s)	2037, 2753
MroXI	GAANNNNTTC	1 cut(s)	352
MscI	TGGCCA	2 cut(s)	2037, 2753
MslI	CAYNNNNRTG	4 cut(s)	1969, 2155, 2605, 2724
Msp20I	TGGCCA	2 cut(s)	2037, 2753
MspA1I	CMGCKG	1 cut(s)	1943
MspCI	CTTAAG	1 cut(s)	793
MspI	CCGG	3 cut(s)	620, 1880, 1957
MspR9I	CCNGG	4 cut(s)	621, 909, 1880, 3275
MssI	GTTTAAAC	1 cut(s)	1816
Mva1269I	GAATGC	1 cut(s)	1825
MvaI	CCWGG	2 cut(s)	909, 3275
MvnI	CGCG	1 cut(s)	2560
MwoI	GCNNNNNNNGC	1 cut(s)	947
NciI	CCSGG	2 cut(s)	621, 1880
NdeI	CATATG	2 cut(s)	991, 2362
NheI	GCTAGC	1 cut(s)	3142
NlaIV	GGNNCC	9 cut(s)	9, 486, 624, 774, 1161, 2069, 2358, 2400, 2656
NmuCI	GTSAC	4 cut(s)	320, 1952, 2312, 2640
NsbI	TGCGCA	1 cut(s)	1192
NspI	RCATGY	4 cut(s)	637, 2313, 3002, 3176
NspV	TTCGAA	1 cut(s)	356
PagI	TCATGA	1 cut(s)	2143
PaqCI	CACCTGC	1 cut(s)	1830
PceI	AGGCCT	1 cut(s)	799
PciI	ACATGT	1 cut(s)	3172
PciSI	GCTCTTC	1 cut(s)	1479
PcsI	WCGNNNNNNNCGW	1 cut(s)	367
PctI	GAATGC	1 cut(s)	1825
PdmI	GAANNNNTTC	1 cut(s)	352
PfeI	GAWTC	4 cut(s)	358, 1883, 2147, 2902
PfoI	TCCNGGA	1 cut(s)	3273
PkrI	GCNGC	3 cut(s)	588, 606, 3148
PleI	GAGTC	9 cut(s)	62, 739, 849, 909, 1252, 1448, 1907, 2102, 2642
PmeI	GTTTAAAC	1 cut(s)	1816
PpsI	GAGTC	9 cut(s)	62, 739, 849, 909, 1252, 1448, 1907, 2102, 2642
PpuMI	RGGWCCY	1 cut(s)	623
PscI	ACATGT	1 cut(s)	3172
PshBI	ATTAAT	1 cut(s)	2139
Psp124BI	GAGCTC	1 cut(s)	1607
Psp1406I	AACGTT	1 cut(s)	2888
Psp5II	RGGWCCY	1 cut(s)	623
Psp6I	CCWGG	2 cut(s)	907, 3273
PspEI	GGTNACC	2 cut(s)	320, 1952
PspGI	CCWGG	2 cut(s)	907, 3273
PspN4I	GGNNCC	9 cut(s)	9, 486, 624, 774, 1161, 2069, 2358, 2400, 2656
PspPI	GGNCC	4 cut(s)	623, 1894, 2366, 2655
PspPPI	RGGWCCY	1 cut(s)	623
PstI	CTGCAG	1 cut(s)	2173
PstNI	CAGNNNCTG	1 cut(s)	2129
PvuII	CAGCTG	1 cut(s)	1943
RsaI	GTAC	9 cut(s)	9, 452, 712, 1356, 1738, 2488, 2806, 2917, 3290
RsaNI	GTAC	9 cut(s)	8, 451, 711, 1355, 1737, 2487, 2805, 2916, 3289
RseI	CAYNNNNRTG	4 cut(s)	1969, 2155, 2605, 2724
SacI	GAGCTC	1 cut(s)	1607
SapI	GCTCTTC	1 cut(s)	1479
SatI	GCNGC	3 cut(s)	587, 605, 3147
Sau96I	GGNCC	4 cut(s)	623, 1894, 2366, 2655
ScaI	AGTACT	1 cut(s)	2806
SchI	GAGTC	9 cut(s)	62, 740, 849, 909, 1252, 1449, 1908, 2102, 2643
ScrFI	CCNGG	4 cut(s)	621, 909, 1880, 3275
SduI	GDGCHC	4 cut(s)	1607, 2510, 2535, 2578
SfaNI	GCATC	3 cut(s)	39, 2667, 3268
SfcI	CTRYAG	7 cut(s)	489, 801, 1263, 1397, 1635, 2169, 2783
SfuI	TTCGAA	1 cut(s)	356
SinI	GGWCC	2 cut(s)	623, 2366
SmiMI	CAYNNNNRTG	4 cut(s)	1969, 2155, 2605, 2724
SmlI	CTYRAG	3 cut(s)	726, 793, 857
SmoI	CTYRAG	3 cut(s)	726, 793, 857
SpeI	ACTAGT	2 cut(s)	2671, 2801
SseBI	AGGCCT	1 cut(s)	799
SsiI	CCGC	4 cut(s)	536, 586, 1588, 2579
SspI	AATATT	1 cut(s)	1599
SspMI	CTAG	8 cut(s)	132, 525, 1407, 2403, 2672, 2802, 3143, 3307
SstI	GAGCTC	1 cut(s)	1607
StuI	AGGCCT	1 cut(s)	799
StyD4I	CCNGG	4 cut(s)	619, 907, 1878, 3273
StyI	CCWWGG	4 cut(s)	524, 837, 2569, 2754
TaaI	ACNGT	8 cut(s)	326, 450, 1078, 1304, 2125, 2702, 2949, 3196
TaiI	ACGT	3 cut(s)	1013, 2891, 3162
TaqII	GACCGA	2 cut(s)	1987, 2275
TatI	WGTACW	3 cut(s)	1736, 2804, 3288
TauI	GCSGC	1 cut(s)	589
TfiI	GAWTC	4 cut(s)	358, 1883, 2147, 2902
TscAI	CASTG	5 cut(s)	271, 1481, 1917, 2501, 3199
TseFI	GTSAC	4 cut(s)	320, 1952, 2312, 2640
TseI	GCWGC	2 cut(s)	604, 3146
Tsp45I	GTSAC	4 cut(s)	320, 1952, 2312, 2640
TspGWI	ACGGA	2 cut(s)	1911, 2895
TspRI	CASTG	5 cut(s)	271, 1481, 1917, 2501, 3199
Vha464I	CTTAAG	1 cut(s)	793
VneI	GTGCAC	1 cut(s)	2574
VpaK11BI	GGWCC	2 cut(s)	623, 2366
VspI	ATTAAT	1 cut(s)	2139
XapI	RAATTY	4 cut(s)	127, 656, 1755, 2049
XceI	RCATGY	4 cut(s)	637, 2313, 3002, 3176
XmaJI	CCTAGG	1 cut(s)	524
XmiI	GTMKAC	1 cut(s)	913
XmnI	GAANNNNTTC	1 cut(s)	352
XspI	CTAG	8 cut(s)	132, 525, 1407, 2403, 2672, 2802, 3143, 3307
ZraI	GACGTC	1 cut(s)	3160
ZrmI	AGTACT	1 cut(s)	2806

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.