MD14G1127400.v1.1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004361
Physical Location & Seq
Reverse (-)
20325195 .. 20326518
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1127400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGAGAACAGTCTTAGATACTAGGAATTGGCCGTTGATTGCTCCGTTGCCATCTTATGGAAGAGGGAGGGAGCTCCCTGGAGGAAGCAACGTTGTTGTCCGGTATGTCACAATATTGGCTCCATCGGACTCTCCCAACACCGATGGAGTAGATCCTGATTCAAGCTCTAATGTCTGCATAGAGGACTCCTACATATCCACTGGAGAGGATCTCGTGGCTGTAAAGAGTGGATGGGATGAGTATGGGATCGCTTACGGTCGCCCAAGTTTTGACATTACCATCCGCCGGATTAGAGGATCATCCCAGTTTGCTGGAATTGCAGTCGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTCCTGGCAGAACACATAAACCGTTACAACATGGGAGTTGGCATCAATATAAAAACAAACATCGGTCGTGGAGGGTTTATAAGAAGCATCACCGTGTCAGACATGTACATGGAAAAAGTACGAAAGGGAGTAAAGATAGCAGGAGATGTTGGAGACCATCCGGATGAAAAATGCAACCAAAACGCTTTCCCAATCGTGACGGGCATCACGTTGAAGGACATCTGGAGCGTGCAGGTTCAGCAGGCCGGTTTACTCCTGGGCCTGAATTTTACCGGAATCTGCCTCTGCAAGATCAACCTCCACGGTGTGACGGGATCAAGAAATGCCTTATGGTTAGCGGGGCTGCACATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

237

Amino Acids

25.41

Weight (kDa)

6.42

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 27 - 196 4.5e-22 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 56
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 2 cut(s) 283, 692
AclI AACGTT 1 cut(s) 90
AclWI GGATC 5 cut(s) 146, 216, 254, 304, 676
AcoI YGGCCR 1 cut(s) 29
AcsI RAATTY 1 cut(s) 619
AdeI CACNNNGTG 1 cut(s) 661
AfaI GTAC 2 cut(s) 461, 474
AfiI CCNNNNNNNGG 2 cut(s) 56, 285
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 2 cut(s) 162, 568
AjnI CCWGG 3 cut(s) 76, 357, 609
AluBI AGCT 2 cut(s) 73, 165
AluI AGCT 2 cut(s) 73, 165
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 5 cut(s) 146, 216, 254, 304, 676
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 3 cut(s) 29, 597, 613
ApeKI GCWGC 1 cut(s) 697
ApoI RAATTY 1 cut(s) 619
AspS9I GGNCC 1 cut(s) 613
AsuHPI GGTGA 1 cut(s) 436
BanII GRGCYC 1 cut(s) 75
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 684
BccI CCATC 6 cut(s) 58, 130, 137, 225, 287, 519
BceAI ACGGC 1 cut(s) 16
BciT130I CCWGG 3 cut(s) 78, 359, 611
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 1 cut(s) 698
BlsI GCNGC 1 cut(s) 699
Bme1390I CCNGG 3 cut(s) 78, 359, 611
BmgT120I GGNCC 1 cut(s) 613
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 78, 359, 611
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 3 cut(s) 405, 450, 567
BmuI ACTGGG 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 3 cut(s) 99, 222, 598
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 76, 610, 655
BsaWI WCCGGW 3 cut(s) 99, 514, 626
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 285
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 2 cut(s) 205, 304
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 78, 359, 611
BseDI CCNNGG 3 cut(s) 76, 610, 655
BseGI GGATG 6 cut(s) 236, 241, 279, 299, 511, 523
BseLI CCNNNNNNNGG 2 cut(s) 56, 285
BseNI ACTGG 2 cut(s) 205, 304
BseXI GCAGC 1 cut(s) 684
BsgI GTGCAG 2 cut(s) 605, 683
Bsh1285I CGRYCG 2 cut(s) 259, 421
BshFI GGCC 3 cut(s) 31, 599, 615
BsiEI CGRYCG 2 cut(s) 259, 421
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 5 cut(s) 100, 286, 515, 600, 627
BslI CCNNNNNNNGG 2 cut(s) 56, 285
BsmAI GTCTC 1 cut(s) 501
BsnI GGCC 3 cut(s) 31, 599, 615
Bso31I GGTCTC 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 459
Bsp143I GATC 6 cut(s) 151, 208, 246, 296, 645, 668
BspACI CCGC 2 cut(s) 283, 692
BspANI GGCC 3 cut(s) 31, 599, 615
BspEI TCCGGA 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 5 cut(s) 146, 216, 254, 304, 676
BspTNI GGTCTC 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 599
BsrGI TGTACA 1 cut(s) 459
BsrI ACTGG 2 cut(s) 205, 304
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 3 cut(s) 76, 610, 655
BssMI GATC 6 cut(s) 151, 208, 246, 296, 645, 668
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 3 cut(s) 78, 359, 611
Bst4CI ACNGT 5 cut(s) 10, 257, 377, 448, 659
Bst6I CTCTTC 1 cut(s) 55
BstAUI TGTACA 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 584, 597
BstDEI CTNAG 1 cut(s) 13
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 6 cut(s) 236, 241, 279, 299, 511, 523
BstKTI GATC 6 cut(s) 154, 211, 249, 299, 648, 671
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 6 cut(s) 151, 208, 246, 296, 645, 668
BstMCI CGRYCG 2 cut(s) 259, 421
BstMWI GCNNNNNNNGC 2 cut(s) 317, 592
BstNI CCWGG 3 cut(s) 78, 359, 611
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 76, 357, 609
BstV1I GCAGC 1 cut(s) 684
BstX2I RGATCY 2 cut(s) 151, 208
BstXI CCANNNNNNTGG 1 cut(s) 311
BstYI RGATCY 2 cut(s) 151, 208
BsuRI GGCC 3 cut(s) 31, 599, 615
BtgI CCRYGG 1 cut(s) 655
BtsCI GGATG 6 cut(s) 236, 241, 279, 299, 511, 523
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 584, 597
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 613
Csp6I GTAC 2 cut(s) 460, 473
CviAII CATG 3 cut(s) 385, 457, 463
CviJI RGCY 8 cut(s) 31, 73, 119, 165, 218, 599, 615, 697
CviKI_1 RGCY 8 cut(s) 31, 73, 119, 165, 218, 599, 615, 697
CviQI GTAC 2 cut(s) 460, 473
DdeI CTNAG 1 cut(s) 13
DpnI GATC 6 cut(s) 153, 210, 248, 298, 647, 670
DpnII GATC 6 cut(s) 151, 208, 246, 296, 645, 668
DraIII CACNNNGTG 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 29
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 272
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 76, 357, 609
EcoT38I GRGCYC 1 cut(s) 75
FaeI CATG 3 cut(s) 388, 460, 466
FatI CATG 3 cut(s) 384, 456, 462
FauI CCCGC 1 cut(s) 685
Fnu4HI GCNGC 1 cut(s) 698
FokI GGATG 6 cut(s) 243, 248, 266, 286, 498, 530
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 698
FspBI CTAG 1 cut(s) 21
GluI GCNGC 1 cut(s) 698
GsuI CTGGAG 3 cut(s) 99, 222, 598
HaeIII GGCC 3 cut(s) 31, 599, 615
HapII CCGG 5 cut(s) 100, 286, 515, 600, 627
Hin1II CATG 3 cut(s) 388, 460, 466
HinfI GANTC 4 cut(s) 128, 158, 185, 630
HpaII CCGG 5 cut(s) 100, 286, 515, 600, 627
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 1 cut(s) 605
Hpy188I TCNGA 3 cut(s) 127, 326, 454
Hpy188III TCNNGA 5 cut(s) 155, 515, 550, 577, 672
Hpy8I GTNNAC 1 cut(s) 605
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 5 cut(s) 10, 257, 377, 448, 659
HpyCH4IV ACGT 2 cut(s) 90, 563
HpyCH4V TGCA 6 cut(s) 177, 320, 528, 586, 642, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 592
HpyF3I CTNAG 1 cut(s) 13
HpySE526I ACGT 2 cut(s) 90, 563
Hsp92II CATG 3 cut(s) 388, 460, 466
Kpn2I TCCGGA 1 cut(s) 514
Kzo9I GATC 6 cut(s) 151, 208, 246, 296, 645, 668
LmnI GCTCC 5 cut(s) 46, 70, 78, 124, 579
Lsp1109I GCAGC 1 cut(s) 684
LweI GCATC 3 cut(s) 405, 450, 567
MaeI CTAG 1 cut(s) 21
MaeII ACGT 2 cut(s) 90, 563
MaeIII GTNAC 4 cut(s) 106, 377, 550, 661
MalI GATC 6 cut(s) 153, 210, 248, 298, 647, 670
MboI GATC 6 cut(s) 151, 208, 246, 296, 645, 668
MboII GAAGA 1 cut(s) 72
MflI RGATCY 2 cut(s) 151, 208
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 25, 315, 619
MlyI GAGTC 2 cut(s) 122, 179
MmeI TCCRAC 2 cut(s) 304, 484
MroI TCCGGA 1 cut(s) 514
MslI CAYNNNNRTG 3 cut(s) 446, 461, 516
MspI CCGG 5 cut(s) 100, 286, 515, 600, 627
MspR9I CCNGG 3 cut(s) 78, 359, 611
MvaI CCWGG 3 cut(s) 78, 359, 611
MwoI GCNNNNNNNGC 2 cut(s) 317, 592
NdeII GATC 6 cut(s) 151, 208, 246, 296, 645, 668
NlaIII CATG 3 cut(s) 388, 460, 466
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 3 cut(s) 106, 550, 661
NspI RCATGY 1 cut(s) 460
PciI ACATGT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PfeI GAWTC 2 cut(s) 158, 630
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 699
PleI GAGTC 2 cut(s) 122, 179
PpsI GAGTC 2 cut(s) 122, 179
PscI ACATGT 1 cut(s) 456
PsiI TTATAA 1 cut(s) 434
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 3 cut(s) 76, 357, 609
PspGI CCWGG 3 cut(s) 76, 357, 609
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 613
PsuI RGATCY 2 cut(s) 151, 208
RsaI GTAC 2 cut(s) 461, 474
RsaNI GTAC 2 cut(s) 460, 473
RseI CAYNNNNRTG 3 cut(s) 446, 461, 516
SacI GAGCTC 1 cut(s) 75
SatI GCNGC 1 cut(s) 698
Sau3AI GATC 6 cut(s) 151, 208, 246, 296, 645, 668
Sau96I GGNCC 1 cut(s) 613
SchI GAGTC 2 cut(s) 122, 179
ScrFI CCNGG 3 cut(s) 78, 359, 611
SduI GDGCHC 1 cut(s) 75
SetI ASST 7 cut(s) 75, 93, 167, 338, 566, 591, 654
SfaNI GCATC 3 cut(s) 405, 450, 567
SmiMI CAYNNNNRTG 3 cut(s) 446, 461, 516
Sse9I AATT 3 cut(s) 25, 315, 619
SsiI CCGC 2 cut(s) 283, 692
SspI AATATT 1 cut(s) 114
SspMI CTAG 1 cut(s) 21
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 76, 357, 609
TaaI ACNGT 5 cut(s) 10, 257, 377, 448, 659
TaiI ACGT 2 cut(s) 93, 566
TaqII GACCGA 1 cut(s) 407
TasI AATT 3 cut(s) 25, 315, 619
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 2 cut(s) 158, 630
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 3 cut(s) 106, 550, 661
TseI GCWGC 1 cut(s) 697
Tsp45I GTSAC 3 cut(s) 106, 550, 661
TspDTI ATGAA 1 cut(s) 534
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 56
XapI RAATTY 1 cut(s) 619
XceI RCATGY 1 cut(s) 460
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.