Rorug07G0020400
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004361
Physical Location & Seq
Forward (+)
1522155 .. 1522595
441 bp
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UTR
Exon/CDS
Intron
Rorug07G0020400.1

Sequence Viewer

Length: 441 bp
ATGGCAAGAGACTTGGGGTGCAAGGTAACTGTGGCGGAGGCTGACATGAATGTGTCGAATTTGGCAAATGTTGTGAATTCTTGTGATGTGATGATGGGAGTTCATGGAGCTGGTCTTTTGAACATGCTTTTCCTACCCGAAAATGCAATTTTTATCCAGATAATTCCAGTTGGAAAGTTCCAATGGATTTCGACAACATACTATGGAGGGCCAGCAAAGGATATGAATCTAAAGTATTTGGAATATAAGATAAGCAATGAAGAGAGCACACTGATACAGCAATACCCACTTGATCATGCAGTTTTTACTGACCCTATCTCAATTGGAAAACAAGGGTGGATGGTATTCAAGTCAATCTATCTGAACAAACAAAATGTAAAGCTTAATGTCACTAGGTTTAAACCCACGTTGTTAAAAGCCCTTGAGCTTTTGCAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

146

Amino Acids

16.42

Weight (kDa)

7.73

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 11 - 51 8.8e-06 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 35
AcsI RAATTY 2 cut(s) 58, 76
AgsI TTSAA 2 cut(s) 121, 349
AluBI AGCT 3 cut(s) 110, 382, 427
AluI AGCT 3 cut(s) 110, 382, 427
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 1 cut(s) 3
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 433
ApoI RAATTY 2 cut(s) 58, 76
AspS9I GGNCC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 269
BccI CCATC 2 cut(s) 88, 334
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 393
BisI GCNGC 1 cut(s) 434
BlsI GCNGC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 225
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 225
BseMI GCAATG 1 cut(s) 262
BseNI ACTGG 1 cut(s) 167
BshFI GGCC 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 3
BsnI GGCC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 269
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 167
BssMI GATC 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 213
BstF5I GGATG 1 cut(s) 345
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 127
BsuRI GGCC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 209
CviAII CATG 4 cut(s) 46, 104, 124, 296
CviJI RGCY 6 cut(s) 41, 110, 211, 382, 419, 427
CviKI_1 RGCY 6 cut(s) 41, 110, 211, 382, 419, 427
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
DraI TTTAAA 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 76
FaeI CATG 4 cut(s) 49, 107, 127, 299
FaiI YATR 8 cut(s) 47, 105, 125, 199, 204, 224, 246, 297
FatI CATG 4 cut(s) 45, 103, 123, 295
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 1 cut(s) 434
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 434
FspBI CTAG 1 cut(s) 393
GluI GCNGC 1 cut(s) 434
HaeIII GGCC 1 cut(s) 211
Hin1II CATG 4 cut(s) 49, 107, 127, 299
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 363
Hpy188III TCNNGA 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 4 cut(s) 21, 146, 299, 433
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 4 cut(s) 49, 107, 127, 299
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 4 cut(s) 96, 170, 180, 225
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 2 cut(s) 25, 388
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 1 cut(s) 272
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 5 cut(s) 58, 76, 147, 162, 321
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 2 cut(s) 31, 200
MseI TTAA 3 cut(s) 384, 399, 413
MslI CAYNNNNRTG 1 cut(s) 50
MssI GTTTAAAC 1 cut(s) 400
MunI CAATTG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 433
NdeII GATC 1 cut(s) 292
NlaIII CATG 4 cut(s) 49, 107, 127, 299
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 127
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 1 cut(s) 435
PmeI GTTTAAAC 1 cut(s) 400
PspPI GGNCC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 3 cut(s) 384, 399, 413
SatI GCNGC 1 cut(s) 434
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 269
SetI ASST 6 cut(s) 27, 112, 384, 398, 410, 429
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 5 cut(s) 58, 76, 147, 162, 321
SsiI CCGC 1 cut(s) 35
SspMI CTAG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 410
TaqI TCGA 2 cut(s) 56, 191
TasI AATT 5 cut(s) 58, 76, 147, 162, 321
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 3 cut(s) 384, 399, 413
Tru9I TTAA 3 cut(s) 384, 399, 413
TscAI CASTG 1 cut(s) 276
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 4 cut(s) 62, 92, 239, 273
TspRI CASTG 1 cut(s) 276
XapI RAATTY 2 cut(s) 58, 76
XceI RCATGY 1 cut(s) 127
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.