Rh6BG026100
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004361
Physical Location & Seq
Reverse (-)
3887018 .. 3890303
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG026100.1

Sequence Viewer

Length: 1053 bp
ATGACTCTGTACTTGGCTAGAGGTGCTGTTATCAAAGCCACACAGGACACTAAGAATTGGCGTTTAATTGCTCCTTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGCGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTCACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATGGGATTGCTTATGGTCACCCTAGTTCCGACATTACCATCCGTCGGATCAGAGGATCATCCCCATTTTCTGGAATTGCAATAGGATGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACACATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAACATCGGTAGGGGAGGCTTCATAAGAAACATAACAGTTTCAAATGTGTTCATGGAAAAGGCACAGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAAGGGTATCACATTAACAGACATCTGGGGTATACAAGTTCTGCAGGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCGGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACCGCAACAACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTCGGAGTTGACCAGTGCCCAACACGCAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

350

Amino Acids

38.03

Weight (kDa)

6.59

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 42 - 323 6.6e-40 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 566
AccI GTMKAC 1 cut(s) 838
AciI CCGC 2 cut(s) 930, 972
AclWI GGATC 3 cut(s) 401, 551, 559
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 779
AfaI GTAC 2 cut(s) 11, 446
AfiI CCNNNNNNNGG 5 cut(s) 86, 540, 566, 890, 1006
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 4 cut(s) 233, 417, 708, 784
AjnI CCWGG 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 3 cut(s) 126, 380, 420
AluI AGCT 3 cut(s) 126, 380, 420
Alw21I GWGCWC 1 cut(s) 962
Alw26I GTCTC 2 cut(s) 756, 863
AlwI GGATC 3 cut(s) 401, 551, 559
AoxI GGCC 2 cut(s) 187, 868
ApeKI GCWGC 1 cut(s) 977
ApoI RAATTY 1 cut(s) 779
AsuHPI GGTGA 6 cut(s) 179, 229, 317, 506, 880, 1000
BaeGI GKGCMC 1 cut(s) 1024
BalI TGGCCA 1 cut(s) 189
BanII GRGCYC 1 cut(s) 995
Bbv12I GWGCWC 1 cut(s) 962
BbvI GCAGC 1 cut(s) 964
BccI CCATC 6 cut(s) 134, 179, 392, 480, 542, 1004
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 108
BciVI GTATCC 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 756, 863
BfaI CTAG 2 cut(s) 18, 519
BfmI CTRYAG 1 cut(s) 848
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 662
BisI GCNGC 1 cut(s) 978
BlsI GCNGC 1 cut(s) 979
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 108
BmrI ACTGGG 1 cut(s) 794
BmuI ACTGGG 1 cut(s) 794
BpmI CTGGAG 3 cut(s) 129, 360, 477
BpuEI CTTGAG 1 cut(s) 857
BsaI GGTCTC 2 cut(s) 756, 863
BsaJI CCNNGG 5 cut(s) 106, 133, 190, 919, 950
BsaWI WCCGGW 2 cut(s) 354, 890
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 540, 566, 890, 1006
Bse1I ACTGG 4 cut(s) 169, 460, 800, 1017
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 5 cut(s) 106, 133, 190, 919, 950
BseGI GGATG 7 cut(s) 129, 491, 496, 534, 554, 587, 996
BseLI CCNNNNNNNGG 5 cut(s) 86, 540, 566, 890, 1006
BseMII CTCAG 1 cut(s) 975
BseNI ACTGG 4 cut(s) 169, 460, 800, 1017
BseSI GKGCMC 1 cut(s) 1024
BseXI GCAGC 1 cut(s) 964
BshFI GGCC 2 cut(s) 189, 870
BsiHKAI GWGCWC 1 cut(s) 962
BsiSI CCGG 2 cut(s) 355, 891
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 5 cut(s) 86, 540, 566, 890, 1006
BsmAI GTCTC 2 cut(s) 756, 863
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 2 cut(s) 189, 870
Bso31I GGTCTC 2 cut(s) 756, 863
Bsp1286I GDGCHC 3 cut(s) 962, 995, 1024
Bsp143I GATC 4 cut(s) 406, 463, 543, 551
Bsp19I CCATGG 3 cut(s) 133, 919, 950
BspACI CCGC 2 cut(s) 930, 972
BspANI GGCC 2 cut(s) 189, 870
BspCNI CTCAG 1 cut(s) 974
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 375
BspMAI CTGCAG 1 cut(s) 852
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 3 cut(s) 401, 551, 559
BspTNI GGTCTC 2 cut(s) 756, 863
BsrI ACTGG 4 cut(s) 169, 460, 800, 1017
BssECI CCNNGG 5 cut(s) 106, 133, 190, 919, 950
BssMI GATC 4 cut(s) 406, 463, 543, 551
BssNAI GTATAC 1 cut(s) 839
BssT1I CCWWGG 4 cut(s) 133, 190, 919, 950
Bst1107I GTATAC 1 cut(s) 839
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 703
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 2 cut(s) 51, 961
BstDSI CCRYGG 3 cut(s) 133, 919, 950
BstEII GGTNACC 1 cut(s) 512
BstF5I GGATG 7 cut(s) 129, 491, 496, 534, 554, 587, 996
BstKTI GATC 4 cut(s) 409, 466, 546, 554
BstMAI GTCTC 2 cut(s) 756, 863
BstMBI GATC 4 cut(s) 406, 463, 543, 551
BstMWI GCNNNNNNNGC 2 cut(s) 23, 1028
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 512
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 848
BstSLI GKGCMC 1 cut(s) 1024
BstV1I GCAGC 1 cut(s) 964
BstXI CCANNNNNNTGG 1 cut(s) 140
BstZ17I GTATAC 1 cut(s) 839
BsuI GTATCC 1 cut(s) 662
BsuRI GGCC 2 cut(s) 189, 870
BtgI CCRYGG 3 cut(s) 133, 919, 950
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 7 cut(s) 129, 491, 496, 534, 554, 587, 996
BtsIMutI CAGTG 3 cut(s) 162, 807, 1024
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 1 cut(s) 852
CciI TCATGA 1 cut(s) 148
Csp6I GTAC 2 cut(s) 10, 445
CviAII CATG 8 cut(s) 134, 149, 211, 718, 920, 925, 951, 1038
CviQI GTAC 2 cut(s) 10, 445
DdeI CTNAG 2 cut(s) 51, 961
DpnI GATC 4 cut(s) 408, 465, 545, 553
DpnII GATC 4 cut(s) 406, 463, 543, 551
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 4 cut(s) 133, 190, 919, 950
Eco147I AGGCCT 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 995
Eco31I GGTCTC 2 cut(s) 756, 863
Eco91I GGTNACC 1 cut(s) 512
EcoO65I GGTNACC 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 4 cut(s) 133, 190, 919, 950
EcoT38I GRGCYC 1 cut(s) 995
ErhI CCWWGG 4 cut(s) 133, 190, 919, 950
FaeI CATG 8 cut(s) 137, 152, 214, 721, 923, 928, 954, 1041
FaqI GGGAC 1 cut(s) 190
FatI CATG 8 cut(s) 133, 148, 210, 717, 919, 924, 950, 1037
FauI CCCGC 1 cut(s) 965
FblI GTMKAC 1 cut(s) 838
Fnu4HI GCNGC 1 cut(s) 978
FokI GGATG 7 cut(s) 116, 498, 503, 521, 541, 594, 983
FriOI GRGCYC 1 cut(s) 995
Fsp4HI GCNGC 1 cut(s) 978
FspBI CTAG 2 cut(s) 18, 519
GluI GCNGC 1 cut(s) 978
GsuI CTGGAG 3 cut(s) 129, 360, 477
HaeIII GGCC 2 cut(s) 189, 870
HapII CCGG 2 cut(s) 355, 891
Hin1II CATG 8 cut(s) 137, 152, 214, 721, 923, 928, 954, 1041
HincII GTYRAC 1 cut(s) 1014
HindII GTYRAC 1 cut(s) 1014
HinfI GANTC 5 cut(s) 4, 383, 413, 440, 894
HpaII CCGG 2 cut(s) 355, 891
HphI GGTGA 6 cut(s) 179, 229, 317, 506, 880, 1000
Hpy166II GTNNAC 2 cut(s) 839, 1014
Hpy188I TCNGA 5 cut(s) 526, 543, 548, 964, 1009
Hpy188III TCNNGA 5 cut(s) 149, 301, 410, 567, 874
Hpy8I GTNNAC 2 cut(s) 839, 1014
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 3 cut(s) 84, 641, 727
HpyCH4III ACNGT 1 cut(s) 703
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 5 cut(s) 432, 575, 749, 794, 850
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 1028
HpyF3I CTNAG 2 cut(s) 51, 961
HpySE526I ACGT 1 cut(s) 639
Hsp92II CATG 8 cut(s) 137, 152, 214, 721, 923, 928, 954, 1041
Kzo9I GATC 4 cut(s) 406, 463, 543, 551
LmnI GCTCC 3 cut(s) 76, 379, 1046
Lsp1109I GCAGC 1 cut(s) 964
MaeI CTAG 2 cut(s) 18, 519
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 2 cut(s) 361, 512
MalI GATC 4 cut(s) 408, 465, 545, 553
MboI GATC 4 cut(s) 406, 463, 543, 551
MboII GAAGA 1 cut(s) 221
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 3 cut(s) 962, 995, 1024
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 7 cut(s) 55, 66, 180, 233, 402, 570, 779
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 2 cut(s) 377, 434
MmeI TCCRAC 4 cut(s) 521, 549, 739, 930
Mox20I TGGCCA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 189
MseI TTAA 2 cut(s) 65, 821
MslI CAYNNNNRTG 4 cut(s) 138, 771, 949, 955
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 2 cut(s) 380, 972
MspI CCGG 2 cut(s) 355, 891
MspR9I CCNGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 23, 1028
NcoI CCATGG 3 cut(s) 133, 919, 950
NdeII GATC 4 cut(s) 406, 463, 543, 551
NlaIII CATG 8 cut(s) 137, 152, 214, 721, 923, 928, 954, 1041
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 361, 512
NspI RCATGY 1 cut(s) 214
PagI TCATGA 1 cut(s) 148
PceI AGGCCT 1 cut(s) 870
PciI ACATGT 1 cut(s) 210
PfeI GAWTC 2 cut(s) 413, 894
PflMI CCANNNNNTGG 1 cut(s) 566
PkrI GCNGC 1 cut(s) 979
PleI GAGTC 2 cut(s) 377, 434
PpsI GAGTC 2 cut(s) 377, 434
PscI ACATGT 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 106
PspEI GGTNACC 1 cut(s) 512
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 375
PstI CTGCAG 1 cut(s) 852
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 2 cut(s) 11, 446
RsaNI GTAC 2 cut(s) 10, 445
RseI CAYNNNNRTG 4 cut(s) 138, 771, 949, 955
SaqAI TTAA 2 cut(s) 65, 821
SatI GCNGC 1 cut(s) 978
Sau3AI GATC 4 cut(s) 406, 463, 543, 551
SchI GAGTC 2 cut(s) 377, 434
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 3 cut(s) 962, 995, 1024
SfcI CTRYAG 1 cut(s) 848
SmiMI CAYNNNNRTG 4 cut(s) 138, 771, 949, 955
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 7 cut(s) 55, 66, 180, 233, 402, 570, 779
SseBI AGGCCT 1 cut(s) 870
SsiI CCGC 2 cut(s) 930, 972
SspI AATATT 2 cut(s) 322, 369
SspMI CTAG 2 cut(s) 18, 519
StuI AGGCCT 1 cut(s) 870
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 4 cut(s) 133, 190, 919, 950
TaaI ACNGT 1 cut(s) 703
TaiI ACGT 1 cut(s) 642
TasI AATT 7 cut(s) 55, 66, 180, 233, 402, 570, 779
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 2 cut(s) 413, 894
Tru1I TTAA 2 cut(s) 65, 821
Tru9I TTAA 2 cut(s) 65, 821
TscAI CASTG 3 cut(s) 169, 807, 1024
TseFI GTSAC 2 cut(s) 361, 512
TseI GCWGC 1 cut(s) 977
Tsp45I GTSAC 2 cut(s) 361, 512
TspDTI ATGAA 6 cut(s) 118, 137, 250, 676, 706, 1026
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 3 cut(s) 169, 807, 1024
Van91I CCANNNNNTGG 1 cut(s) 566
XapI RAATTY 1 cut(s) 779
XceI RCATGY 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 838
XspI CTAG 2 cut(s) 18, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.