Rh6DG024300
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004361
Physical Location & Seq
Reverse (-)
2199013 .. 2203525
4513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG024300.1

Sequence Viewer

Length: 1089 bp
ATGAAAGTTGGAGAATGGGTCTTCGTCGCAATCGCCGTCGTTTTGAACTTGGCTAGAGGTGCTGTTATCAAAGCCACACAGGACACTAAGAATTGGCGTTTAATTGCTCCTTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGCGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTCACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATGGGATTGCTTATGGTCACCCTAGTTCCGACATTACCATCCGTCGGATCAGAGGATCATCCCCATTTTCTGGAATTGCAATAGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACACATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAACATCGGTAGGGGAGGCTTCATAAGAAACATAACAGTTTCAAATGTGTTCATGGAAAAGGCACGGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAAGGGTATCACATTAACAGACATCTGGGGTATACAAGTTCTGCAGGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCGGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACCGCAACAACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTCGGAGTTGACCAGTGCCCAACACACAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

362

Amino Acids

39.32

Weight (kDa)

6.83

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 54 - 335 6.2e-41 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 602
AccI GTMKAC 1 cut(s) 874
AciI CCGC 2 cut(s) 966, 1008
AclWI GGATC 3 cut(s) 437, 587, 595
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 815
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 5 cut(s) 122, 576, 602, 926, 1042
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 5 cut(s) 46, 269, 453, 744, 820
AjnI CCWGG 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 162, 416, 456
AluI AGCT 3 cut(s) 162, 416, 456
Alw21I GWGCWC 1 cut(s) 998
Alw26I GTCTC 2 cut(s) 792, 899
AlwI GGATC 3 cut(s) 437, 587, 595
AoxI GGCC 2 cut(s) 223, 904
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 1 cut(s) 815
AsuHPI GGTGA 6 cut(s) 215, 265, 353, 542, 916, 1036
BaeGI GKGCMC 1 cut(s) 1060
BalI TGGCCA 1 cut(s) 225
BanII GRGCYC 1 cut(s) 1031
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 998
BbvI GCAGC 1 cut(s) 1000
BccI CCATC 6 cut(s) 170, 215, 428, 516, 578, 1040
BceAI ACGGC 2 cut(s) 20, 100
BciT130I CCWGG 1 cut(s) 144
BciVI GTATCC 1 cut(s) 698
BcoDI GTCTC 2 cut(s) 792, 899
BfaI CTAG 2 cut(s) 54, 555
BfmI CTRYAG 1 cut(s) 884
BfuAI ACCTGC 1 cut(s) 881
BfuI GTATCC 1 cut(s) 698
BisI GCNGC 1 cut(s) 1014
BlsI GCNGC 1 cut(s) 1015
Bme1390I CCNGG 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 830
BmuI ACTGGG 1 cut(s) 830
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 3 cut(s) 165, 396, 513
BpuEI CTTGAG 1 cut(s) 893
BsaI GGTCTC 2 cut(s) 792, 899
BsaJI CCNNGG 5 cut(s) 142, 169, 226, 955, 986
BsaWI WCCGGW 2 cut(s) 390, 926
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 576, 602, 926, 1042
Bse1I ACTGG 4 cut(s) 205, 496, 836, 1053
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 5 cut(s) 142, 169, 226, 955, 986
BseGI GGATG 6 cut(s) 165, 527, 532, 570, 590, 1032
BseLI CCNNNNNNNGG 5 cut(s) 122, 576, 602, 926, 1042
BseMII CTCAG 1 cut(s) 1011
BseNI ACTGG 4 cut(s) 205, 496, 836, 1053
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 1 cut(s) 1000
BshFI GGCC 2 cut(s) 225, 906
BsiHKAI GWGCWC 1 cut(s) 998
BsiSI CCGG 2 cut(s) 391, 927
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 5 cut(s) 122, 576, 602, 926, 1042
BsmAI GTCTC 2 cut(s) 792, 899
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 2 cut(s) 225, 906
Bso31I GGTCTC 2 cut(s) 792, 899
Bsp1286I GDGCHC 3 cut(s) 998, 1031, 1060
Bsp143I GATC 4 cut(s) 442, 499, 579, 587
Bsp19I CCATGG 3 cut(s) 169, 955, 986
BspACI CCGC 2 cut(s) 966, 1008
BspANI GGCC 2 cut(s) 225, 906
BspCNI CTCAG 1 cut(s) 1010
BspHI TCATGA 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 411
BspMAI CTGCAG 1 cut(s) 888
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 3 cut(s) 437, 587, 595
BspTNI GGTCTC 2 cut(s) 792, 899
BsrI ACTGG 4 cut(s) 205, 496, 836, 1053
BssECI CCNNGG 5 cut(s) 142, 169, 226, 955, 986
BssMI GATC 4 cut(s) 442, 499, 579, 587
BssNAI GTATAC 1 cut(s) 875
BssT1I CCWWGG 4 cut(s) 169, 226, 955, 986
Bst1107I GTATAC 1 cut(s) 875
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 2 cut(s) 87, 997
BstDSI CCRYGG 3 cut(s) 169, 955, 986
BstEII GGTNACC 1 cut(s) 548
BstF5I GGATG 6 cut(s) 165, 527, 532, 570, 590, 1032
BstKTI GATC 4 cut(s) 445, 502, 582, 590
BstMAI GTCTC 2 cut(s) 792, 899
BstMBI GATC 4 cut(s) 442, 499, 579, 587
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 250
BstPI GGTNACC 1 cut(s) 548
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 884
BstSLI GKGCMC 1 cut(s) 1060
BstV1I GCAGC 1 cut(s) 1000
BstV2I GAAGAC 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 176
BstZ17I GTATAC 1 cut(s) 875
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 2 cut(s) 225, 906
BtgI CCRYGG 3 cut(s) 169, 955, 986
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 6 cut(s) 165, 527, 532, 570, 590, 1032
BtsIMutI CAGTG 3 cut(s) 198, 843, 1060
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 1 cut(s) 888
CciI TCATGA 1 cut(s) 184
Csp6I GTAC 1 cut(s) 481
CviAII CATG 8 cut(s) 170, 185, 247, 754, 956, 961, 987, 1074
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 2 cut(s) 87, 997
DpnI GATC 4 cut(s) 444, 501, 581, 589
DpnII GATC 4 cut(s) 442, 499, 579, 587
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 4 cut(s) 169, 226, 955, 986
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 1031
Eco31I GGTCTC 2 cut(s) 792, 899
Eco91I GGTNACC 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 4 cut(s) 169, 226, 955, 986
EcoT38I GRGCYC 1 cut(s) 1031
ErhI CCWWGG 4 cut(s) 169, 226, 955, 986
FaeI CATG 8 cut(s) 173, 188, 250, 757, 959, 964, 990, 1077
FaqI GGGAC 1 cut(s) 226
FatI CATG 8 cut(s) 169, 184, 246, 753, 955, 960, 986, 1073
FauI CCCGC 1 cut(s) 1001
FblI GTMKAC 1 cut(s) 874
Fnu4HI GCNGC 1 cut(s) 1014
FokI GGATG 6 cut(s) 152, 534, 539, 557, 577, 1019
FriOI GRGCYC 1 cut(s) 1031
Fsp4HI GCNGC 1 cut(s) 1014
FspBI CTAG 2 cut(s) 54, 555
GluI GCNGC 1 cut(s) 1014
GsuI CTGGAG 3 cut(s) 165, 396, 513
HaeIII GGCC 2 cut(s) 225, 906
HapII CCGG 2 cut(s) 391, 927
Hin1II CATG 8 cut(s) 173, 188, 250, 757, 959, 964, 990, 1077
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HinfI GANTC 4 cut(s) 419, 449, 476, 930
HpaII CCGG 2 cut(s) 391, 927
HphI GGTGA 6 cut(s) 215, 265, 353, 542, 916, 1036
Hpy166II GTNNAC 2 cut(s) 875, 1050
Hpy188I TCNGA 5 cut(s) 562, 579, 584, 1000, 1045
Hpy188III TCNNGA 5 cut(s) 185, 337, 446, 603, 910
Hpy8I GTNNAC 2 cut(s) 875, 1050
Hpy99I CGWCG 3 cut(s) 29, 41, 579
HpyAV CCTTC 3 cut(s) 120, 677, 763
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 5 cut(s) 468, 611, 785, 830, 886
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 87, 997
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 8 cut(s) 173, 188, 250, 757, 959, 964, 990, 1077
Kzo9I GATC 4 cut(s) 442, 499, 579, 587
LmnI GCTCC 3 cut(s) 112, 415, 1082
Lsp1109I GCAGC 1 cut(s) 1000
MaeI CTAG 2 cut(s) 54, 555
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 2 cut(s) 397, 548
MalI GATC 4 cut(s) 444, 501, 581, 589
MboI GATC 4 cut(s) 442, 499, 579, 587
MboII GAAGA 2 cut(s) 13, 257
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 3 cut(s) 998, 1031, 1060
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 7 cut(s) 91, 102, 216, 269, 438, 606, 815
MluNI TGGCCA 1 cut(s) 225
MlyI GAGTC 2 cut(s) 413, 470
MmeI TCCRAC 4 cut(s) 557, 585, 775, 966
Mox20I TGGCCA 1 cut(s) 225
MscI TGGCCA 1 cut(s) 225
MseI TTAA 2 cut(s) 101, 857
MslI CAYNNNNRTG 4 cut(s) 174, 807, 985, 991
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 416, 1008
MspI CCGG 2 cut(s) 391, 927
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 59
NcoI CCATGG 3 cut(s) 169, 955, 986
NdeII GATC 4 cut(s) 442, 499, 579, 587
NlaIII CATG 8 cut(s) 173, 188, 250, 757, 959, 964, 990, 1077
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 2 cut(s) 397, 548
NspI RCATGY 1 cut(s) 250
PagI TCATGA 1 cut(s) 184
PceI AGGCCT 1 cut(s) 906
PciI ACATGT 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 2 cut(s) 449, 930
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 1 cut(s) 1015
PleI GAGTC 2 cut(s) 413, 470
PpsI GAGTC 2 cut(s) 413, 470
PscI ACATGT 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 411
PstI CTGCAG 1 cut(s) 888
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 4 cut(s) 174, 807, 985, 991
SaqAI TTAA 2 cut(s) 101, 857
SatI GCNGC 1 cut(s) 1014
Sau3AI GATC 4 cut(s) 442, 499, 579, 587
SchI GAGTC 2 cut(s) 413, 470
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 3 cut(s) 998, 1031, 1060
SfcI CTRYAG 1 cut(s) 884
SmiMI CAYNNNNRTG 4 cut(s) 174, 807, 985, 991
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
Sse9I AATT 7 cut(s) 91, 102, 216, 269, 438, 606, 815
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 2 cut(s) 966, 1008
SspI AATATT 2 cut(s) 358, 405
SspMI CTAG 2 cut(s) 54, 555
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 4 cut(s) 169, 226, 955, 986
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 1 cut(s) 678
TasI AATT 7 cut(s) 91, 102, 216, 269, 438, 606, 815
TfiI GAWTC 2 cut(s) 449, 930
Tru1I TTAA 2 cut(s) 101, 857
Tru9I TTAA 2 cut(s) 101, 857
TscAI CASTG 3 cut(s) 205, 843, 1060
TseFI GTSAC 2 cut(s) 397, 548
TseI GCWGC 1 cut(s) 1013
Tsp45I GTSAC 2 cut(s) 397, 548
TspDTI ATGAA 7 cut(s) 17, 154, 173, 286, 712, 742, 1062
TspGWI ACGGA 2 cut(s) 563, 781
TspRI CASTG 3 cut(s) 205, 843, 1060
Van91I CCANNNNNTGG 1 cut(s) 602
XapI RAATTY 1 cut(s) 815
XceI RCATGY 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 874
XspI CTAG 2 cut(s) 54, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.