Rw6G002480
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004361
Physical Location & Seq
Reverse (-)
4037204 .. 4040814
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002480.1

Sequence Viewer

Length: 1317 bp
ATGCCTTCTGCTTTCTGGTTCTTCCTTTCGATTTTCCTATGCCTCAGCTACGTCTCTACGGCGGCGGAGCCGGTGACCTGCTCAGGGATAGTGCCAATGAAGTACCGTTACGATAAGATTTCGATAGCGGATTTTGGGGGCCATTTGCACAGGAGAGGAGGCACGCTTCTTTACATACCTCCTGGGGTGTACTTAACGGAGAGCTTCAATCTCACTAGCCACATGACTCTCTACTTGGCTAGAGGTGTTGTTATCAAAGCCACACAGGACACTAAGAATTGGCGTTTAATTGCTCCTTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGCGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTCACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGCACTTATATTTATAACATGTATTTTGTATATCCCTTGTACACATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATGGGATTGCTTATGGTCACCCTAGTTCCGACATTACCATCCGTCGGATCAGAGGATCATCTCCATTTTCTGGAATTGCAATAGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACACATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAATATCGGTAGGGGAGGCTTCATAAGAAACATGACAGTTTCAAATGTGTTCATGGAAAAGGCACGGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAAGGGTATCACATTAACAGACATCTGGGGTGTACAAGTTCTGCATGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCAGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACCGCAACGACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTCGGAGTTGACCAGTGCCCAACACGCAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

438

Amino Acids

48.04

Weight (kDa)

8.05

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 116 - 411 2.2e-33 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
Acc36I ACCTGC 2 cut(s) 86, 1109
AccB7I CCANNNNNTGG 1 cut(s) 830
AciI CCGC 5 cut(s) 62, 65, 128, 1194, 1236
AclWI GGATC 3 cut(s) 623, 815, 823
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 1 cut(s) 1043
AfaI GTAC 5 cut(s) 104, 191, 673, 710, 1104
AfiI CCNNNNNNNGG 5 cut(s) 84, 308, 804, 830, 1270
AflIII ACRYGT 2 cut(s) 432, 650
AgsI TTSAA 5 cut(s) 208, 455, 681, 972, 1048
AjnI CCWGG 2 cut(s) 181, 328
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 5 cut(s) 48, 204, 348, 602, 684
AluI AGCT 5 cut(s) 48, 204, 348, 602, 684
Alw21I GWGCWC 1 cut(s) 1226
Alw26I GTCTC 3 cut(s) 58, 1020, 1127
AlwI GGATC 3 cut(s) 623, 815, 823
AlwNI CAGNNNCTG 1 cut(s) 1161
AoxI GGCC 3 cut(s) 139, 409, 1132
ApeKI GCWGC 1 cut(s) 1241
ApoI RAATTY 1 cut(s) 1043
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 7 cut(s) 85, 401, 451, 539, 770, 1144, 1264
BaeGI GKGCMC 1 cut(s) 1288
BalI TGGCCA 1 cut(s) 411
BanII GRGCYC 1 cut(s) 1259
BarI GAAGNNNNNNTAC 2 cut(s) 92, 124
Bbv12I GWGCWC 1 cut(s) 1226
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 1228
BccI CCATC 6 cut(s) 356, 401, 614, 744, 806, 1268
BceAI ACGGC 2 cut(s) 75, 286
BciT130I CCWGG 2 cut(s) 183, 330
BciVI GTATCC 1 cut(s) 926
BcoDI GTCTC 3 cut(s) 58, 1020, 1127
BfaI CTAG 3 cut(s) 216, 240, 783
BfuAI ACCTGC 2 cut(s) 86, 1109
BfuI GTATCC 1 cut(s) 926
BisI GCNGC 2 cut(s) 63, 1242
BlsI GCNGC 2 cut(s) 64, 1243
Bme1390I CCNGG 2 cut(s) 183, 330
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 3 cut(s) 69, 140, 597
BmrFI CCNGG 2 cut(s) 183, 330
BmrI ACTGGG 1 cut(s) 1058
BmuI ACTGGG 1 cut(s) 1058
BpmI CTGGAG 3 cut(s) 351, 582, 741
Bpu10I CCTNAGC 2 cut(s) 44, 82
BpuEI CTTGAG 1 cut(s) 1121
BsaI GGTCTC 2 cut(s) 1020, 1127
BsaJI CCNNGG 6 cut(s) 182, 328, 355, 412, 1183, 1214
BsaWI WCCGGW 1 cut(s) 576
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 308, 804, 830, 1270
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 4 cut(s) 391, 724, 1064, 1281
BseBI CCWGG 2 cut(s) 183, 330
BseDI CCNNGG 6 cut(s) 182, 328, 355, 412, 1183, 1214
BseGI GGATG 5 cut(s) 351, 755, 760, 798, 1260
BseLI CCNNNNNNNGG 5 cut(s) 84, 308, 804, 830, 1270
BseMII CTCAG 3 cut(s) 58, 96, 1239
BseNI ACTGG 4 cut(s) 391, 724, 1064, 1281
BseRI GAGGAG 1 cut(s) 171
BseSI GKGCMC 1 cut(s) 1288
BseXI GCAGC 1 cut(s) 1228
BshFI GGCC 3 cut(s) 141, 411, 1134
BsiHKAI GWGCWC 1 cut(s) 1226
BsiSI CCGG 2 cut(s) 71, 577
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 5 cut(s) 84, 308, 804, 830, 1270
BsmAI GTCTC 3 cut(s) 58, 1020, 1127
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 3 cut(s) 141, 411, 1134
Bso31I GGTCTC 2 cut(s) 1020, 1127
Bsp1286I GDGCHC 3 cut(s) 1226, 1259, 1288
Bsp1407I TGTACA 2 cut(s) 671, 1102
Bsp143I GATC 4 cut(s) 628, 727, 807, 815
Bsp19I CCATGG 3 cut(s) 355, 1183, 1214
BspACI CCGC 5 cut(s) 62, 65, 128, 1194, 1236
BspANI GGCC 3 cut(s) 141, 411, 1134
BspCNI CTCAG 3 cut(s) 57, 95, 1238
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 69, 140, 597
BspMI ACCTGC 2 cut(s) 86, 1109
BspPI GGATC 3 cut(s) 623, 815, 823
BspTNI GGTCTC 2 cut(s) 1020, 1127
BsrFI RCCGGY 1 cut(s) 70
BsrGI TGTACA 2 cut(s) 671, 1102
BsrI ACTGG 4 cut(s) 391, 724, 1064, 1281
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 6 cut(s) 182, 328, 355, 412, 1183, 1214
BssMI GATC 4 cut(s) 628, 727, 807, 815
BssT1I CCWWGG 4 cut(s) 355, 412, 1183, 1214
Bst2UI CCWGG 2 cut(s) 183, 330
Bst4CI ACNGT 2 cut(s) 107, 967
Bst6I CTCTTC 1 cut(s) 456
BstAUI TGTACA 2 cut(s) 671, 1102
BstC8I GCNNGC 2 cut(s) 164, 1116
BstDEI CTNAG 4 cut(s) 44, 82, 273, 1225
BstDSI CCRYGG 3 cut(s) 355, 1183, 1214
BstEII GGTNACC 2 cut(s) 73, 776
BstF5I GGATG 5 cut(s) 351, 755, 760, 798, 1260
BstKTI GATC 4 cut(s) 631, 730, 810, 818
BstMAI GTCTC 3 cut(s) 58, 1020, 1127
BstMBI GATC 4 cut(s) 628, 727, 807, 815
BstMWI GCNNNNNNNGC 1 cut(s) 1292
BstNI CCWGG 2 cut(s) 183, 330
BstNSI RCATGY 3 cut(s) 436, 654, 1118
BstPI GGTNACC 2 cut(s) 73, 776
BstSCI CCNGG 2 cut(s) 181, 328
BstSLI GKGCMC 1 cut(s) 1288
BstV1I GCAGC 1 cut(s) 1228
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuI GTATCC 1 cut(s) 926
BsuRI GGCC 3 cut(s) 141, 411, 1134
BtgI CCRYGG 3 cut(s) 355, 1183, 1214
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 5 cut(s) 351, 755, 760, 798, 1260
BtsIMutI CAGTG 3 cut(s) 384, 1071, 1288
BveI ACCTGC 2 cut(s) 86, 1109
Cac8I GCNNGC 2 cut(s) 164, 1116
CaiI CAGNNNCTG 1 cut(s) 1161
CciI TCATGA 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 5 cut(s) 103, 190, 672, 709, 1103
CviQI GTAC 5 cut(s) 103, 190, 672, 709, 1103
DdeI CTNAG 4 cut(s) 44, 82, 273, 1225
DpnI GATC 4 cut(s) 630, 729, 809, 817
DpnII GATC 4 cut(s) 628, 727, 807, 815
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 4 cut(s) 355, 412, 1183, 1214
Eco147I AGGCCT 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 1259
Eco31I GGTCTC 2 cut(s) 1020, 1127
Eco91I GGTNACC 2 cut(s) 73, 776
EcoO65I GGTNACC 2 cut(s) 73, 776
EcoRII CCWGG 2 cut(s) 181, 328
EcoT14I CCWWGG 4 cut(s) 355, 412, 1183, 1214
EcoT38I GRGCYC 1 cut(s) 1259
ErhI CCWWGG 4 cut(s) 355, 412, 1183, 1214
Esp3I CGTCTC 1 cut(s) 58
FaqI GGGAC 1 cut(s) 412
FauI CCCGC 1 cut(s) 1229
Fnu4HI GCNGC 2 cut(s) 63, 1242
FokI GGATG 5 cut(s) 338, 762, 767, 785, 1247
FriOI GRGCYC 1 cut(s) 1259
Fsp4HI GCNGC 2 cut(s) 63, 1242
FspBI CTAG 3 cut(s) 216, 240, 783
GluI GCNGC 2 cut(s) 63, 1242
GsuI CTGGAG 3 cut(s) 351, 582, 741
HaeIII GGCC 3 cut(s) 141, 411, 1134
HapII CCGG 2 cut(s) 71, 577
HincII GTYRAC 1 cut(s) 1278
HindII GTYRAC 1 cut(s) 1278
HinfI GANTC 4 cut(s) 226, 605, 704, 1158
HpaII CCGG 2 cut(s) 71, 577
HphI GGTGA 7 cut(s) 85, 401, 451, 539, 770, 1144, 1264
Hpy166II GTNNAC 4 cut(s) 190, 674, 1103, 1278
Hpy188I TCNGA 5 cut(s) 790, 807, 812, 1228, 1273
Hpy188III TCNNGA 4 cut(s) 371, 523, 831, 1138
Hpy8I GTNNAC 4 cut(s) 190, 674, 1103, 1278
Hpy99I CGWCG 1 cut(s) 807
HpyAV CCTTC 4 cut(s) 15, 306, 905, 991
HpyCH4III ACNGT 2 cut(s) 107, 967
HpyCH4IV ACGT 2 cut(s) 51, 903
HpyCH4V TGCA 7 cut(s) 148, 696, 839, 1013, 1058, 1114, 1118
HpyF10VI GCNNNNNNNGC 1 cut(s) 1292
HpyF3I CTNAG 4 cut(s) 44, 82, 273, 1225
HpySE526I ACGT 2 cut(s) 51, 903
Kzo9I GATC 4 cut(s) 628, 727, 807, 815
LmnI GCTCC 4 cut(s) 67, 298, 601, 1310
Lsp1109I GCAGC 1 cut(s) 1228
MaeI CTAG 3 cut(s) 216, 240, 783
MaeII ACGT 2 cut(s) 51, 903
MaeIII GTNAC 4 cut(s) 73, 107, 583, 776
MalI GATC 4 cut(s) 630, 729, 809, 817
MboI GATC 4 cut(s) 628, 727, 807, 815
MboII GAAGA 2 cut(s) 13, 443
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 3 cut(s) 1226, 1259, 1288
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 7 cut(s) 277, 288, 402, 455, 624, 834, 1043
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 3 cut(s) 220, 599, 698
MmeI TCCRAC 4 cut(s) 785, 813, 1003, 1194
Mox20I TGGCCA 1 cut(s) 411
MscI TGGCCA 1 cut(s) 411
MseI TTAA 3 cut(s) 194, 287, 1085
MslI CAYNNNNRTG 4 cut(s) 360, 1035, 1213, 1219
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 602, 1236
MspI CCGG 2 cut(s) 71, 577
MspR9I CCNGG 2 cut(s) 183, 330
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 2 cut(s) 183, 330
MwoI GCNNNNNNNGC 1 cut(s) 1292
NcoI CCATGG 3 cut(s) 355, 1183, 1214
NdeII GATC 4 cut(s) 628, 727, 807, 815
NlaIV GGNNCC 3 cut(s) 69, 140, 597
NmuCI GTSAC 3 cut(s) 73, 583, 776
NspI RCATGY 3 cut(s) 436, 654, 1118
PaeI GCATGC 1 cut(s) 1118
PagI TCATGA 1 cut(s) 370
PceI AGGCCT 1 cut(s) 1134
PciI ACATGT 2 cut(s) 432, 650
PfeI GAWTC 1 cut(s) 1158
PflMI CCANNNNNTGG 1 cut(s) 830
PkrI GCNGC 2 cut(s) 64, 1243
PleI GAGTC 3 cut(s) 220, 599, 698
PpsI GAGTC 3 cut(s) 220, 599, 698
PscI ACATGT 2 cut(s) 432, 650
PsiI TTATAA 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 181, 328
PspEI GGTNACC 2 cut(s) 73, 776
PspGI CCWGG 2 cut(s) 181, 328
PspN4I GGNNCC 3 cut(s) 69, 140, 597
PspPI GGNCC 1 cut(s) 139
PstNI CAGNNNCTG 1 cut(s) 1161
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 5 cut(s) 104, 191, 673, 710, 1104
RsaNI GTAC 5 cut(s) 103, 190, 672, 709, 1103
RseI CAYNNNNRTG 4 cut(s) 360, 1035, 1213, 1219
SaqAI TTAA 3 cut(s) 194, 287, 1085
SatI GCNGC 2 cut(s) 63, 1242
Sau3AI GATC 4 cut(s) 628, 727, 807, 815
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 3 cut(s) 220, 599, 698
ScrFI CCNGG 2 cut(s) 183, 330
SduI GDGCHC 3 cut(s) 1226, 1259, 1288
SmiMI CAYNNNNRTG 4 cut(s) 360, 1035, 1213, 1219
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
SphI GCATGC 1 cut(s) 1118
Sse9I AATT 7 cut(s) 277, 288, 402, 455, 624, 834, 1043
SseBI AGGCCT 1 cut(s) 1134
SsiI CCGC 5 cut(s) 62, 65, 128, 1194, 1236
SspI AATATT 2 cut(s) 544, 591
SspMI CTAG 3 cut(s) 216, 240, 783
StuI AGGCCT 1 cut(s) 1134
StyD4I CCNGG 2 cut(s) 181, 328
StyI CCWWGG 4 cut(s) 355, 412, 1183, 1214
TaaI ACNGT 2 cut(s) 107, 967
TaiI ACGT 2 cut(s) 54, 906
TaqI TCGA 2 cut(s) 29, 122
TasI AATT 7 cut(s) 277, 288, 402, 455, 624, 834, 1043
TatI WGTACW 3 cut(s) 189, 671, 1102
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 1158
Tru1I TTAA 3 cut(s) 194, 287, 1085
Tru9I TTAA 3 cut(s) 194, 287, 1085
TscAI CASTG 3 cut(s) 391, 1071, 1288
TseFI GTSAC 3 cut(s) 73, 583, 776
TseI GCWGC 1 cut(s) 1241
Tsp45I GTSAC 3 cut(s) 73, 583, 776
TspDTI ATGAA 7 cut(s) 113, 340, 359, 472, 940, 970, 1290
TspGWI ACGGA 3 cut(s) 212, 791, 1009
TspRI CASTG 3 cut(s) 391, 1071, 1288
Van91I CCANNNNNTGG 1 cut(s) 830
XapI RAATTY 1 cut(s) 1043
XceI RCATGY 3 cut(s) 436, 654, 1118
XspI CTAG 3 cut(s) 216, 240, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.