Rh6BG026400
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004361
Physical Location & Seq
Reverse (-)
3927490 .. 3943479
15990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG026400.1

Sequence Viewer

Length: 1137 bp
ATGCAGTTTACTTCTCAAGTTCAAGTTTTCACTCATATTCAGCCTAAAGAAGATCTCAAGTCCCTGTTTGCGTCCTTGGCTGCTCAGTCACACAGCGACGGCCAATTCATCTATCCTTCGGTTTTTCAGGACACTAAGAATTGGCGTTTAATTGCTCATTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGTGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTCACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATGGGATTGCTTATGGTCACCCTAGTTCCGACATTACCATCCGTCGGATCAGAGGATCATCCCCATTTTCTGGAATTGCAATAGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACACATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAACATCGGTAGGGGAGGCTTCATAAGAAACATAACAGTTTCAAATGTGTTCATGGAAAAGGCACGGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAAGGGTATCACATTAACAGACATTTGGGGTATACAAGTTCTGCAGGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCGGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACCGCAACAACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTCGGAGTTGACCAGTGCCCAACACGGAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

378

Amino Acids

41.32

Weight (kDa)

6.38

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 351 5.6e-41 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 929
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 1 cut(s) 922
AciI CCGC 2 cut(s) 1014, 1056
AclWI GGATC 3 cut(s) 485, 635, 643
AcoI YGGCCR 2 cut(s) 100, 271
AcsI RAATTY 1 cut(s) 863
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 5 cut(s) 170, 624, 650, 974, 1090
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 5 cut(s) 23, 317, 501, 792, 868
AjnI CCWGG 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 3 cut(s) 210, 464, 504
AluI AGCT 3 cut(s) 210, 464, 504
Alw21I GWGCWC 1 cut(s) 1046
Alw26I GTCTC 2 cut(s) 840, 947
AlwI GGATC 3 cut(s) 485, 635, 643
AoxI GGCC 3 cut(s) 100, 271, 952
ApeKI GCWGC 2 cut(s) 80, 1061
ApoI RAATTY 1 cut(s) 863
AsuHPI GGTGA 7 cut(s) 233, 263, 313, 401, 590, 964, 1084
BaeGI GKGCMC 1 cut(s) 1108
BalI TGGCCA 1 cut(s) 273
BanII GRGCYC 1 cut(s) 1079
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 2 cut(s) 67, 1048
BccI CCATC 6 cut(s) 218, 263, 476, 564, 626, 1088
BceAI ACGGC 2 cut(s) 115, 148
BciT130I CCWGG 1 cut(s) 192
BciVI GTATCC 1 cut(s) 746
BcoDI GTCTC 2 cut(s) 840, 947
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 932
BfuAI ACCTGC 1 cut(s) 929
BfuI GTATCC 1 cut(s) 746
BglII AGATCT 1 cut(s) 52
BisI GCNGC 2 cut(s) 81, 1062
BlsI GCNGC 2 cut(s) 82, 1063
Bme1390I CCNGG 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 878
BpmI CTGGAG 3 cut(s) 213, 444, 561
BpuEI CTTGAG 2 cut(s) 41, 941
BsaI GGTCTC 2 cut(s) 840, 947
BsaJI CCNNGG 6 cut(s) 75, 190, 217, 274, 1003, 1034
BsaWI WCCGGW 2 cut(s) 438, 974
Bsc4I CCNNNNNNNGG 5 cut(s) 170, 624, 650, 974, 1090
Bse1I ACTGG 4 cut(s) 253, 544, 884, 1101
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 6 cut(s) 75, 190, 217, 274, 1003, 1034
BseGI GGATG 6 cut(s) 213, 575, 580, 618, 638, 1080
BseLI CCNNNNNNNGG 5 cut(s) 170, 624, 650, 974, 1090
BseMII CTCAG 2 cut(s) 98, 1059
BseNI ACTGG 4 cut(s) 253, 544, 884, 1101
BseSI GKGCMC 1 cut(s) 1108
BseXI GCAGC 2 cut(s) 67, 1048
BshFI GGCC 3 cut(s) 102, 273, 954
BsiHKAI GWGCWC 1 cut(s) 1046
BsiSI CCGG 2 cut(s) 439, 975
BslFI GGGAC 2 cut(s) 46, 274
BslI CCNNNNNNNGG 5 cut(s) 170, 624, 650, 974, 1090
BsmAI GTCTC 2 cut(s) 840, 947
BsmFI GGGAC 2 cut(s) 46, 274
BsnI GGCC 3 cut(s) 102, 273, 954
Bso31I GGTCTC 2 cut(s) 840, 947
Bsp1286I GDGCHC 3 cut(s) 1046, 1079, 1108
Bsp143I GATC 5 cut(s) 52, 490, 547, 627, 635
Bsp19I CCATGG 3 cut(s) 217, 1003, 1034
BspACI CCGC 2 cut(s) 1014, 1056
BspANI GGCC 3 cut(s) 102, 273, 954
BspCNI CTCAG 2 cut(s) 97, 1058
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 459
BspMAI CTGCAG 1 cut(s) 936
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 3 cut(s) 485, 635, 643
BspTNI GGTCTC 2 cut(s) 840, 947
BsrI ACTGG 4 cut(s) 253, 544, 884, 1101
BssECI CCNNGG 6 cut(s) 75, 190, 217, 274, 1003, 1034
BssMI GATC 5 cut(s) 52, 490, 547, 627, 635
BssNAI GTATAC 1 cut(s) 923
BssT1I CCWWGG 5 cut(s) 75, 217, 274, 1003, 1034
Bst1107I GTATAC 1 cut(s) 923
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 936
BstDEI CTNAG 3 cut(s) 84, 135, 1045
BstDSI CCRYGG 3 cut(s) 217, 1003, 1034
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 6 cut(s) 213, 575, 580, 618, 638, 1080
BstKTI GATC 5 cut(s) 55, 493, 550, 630, 638
BstMAI GTCTC 2 cut(s) 840, 947
BstMBI GATC 5 cut(s) 52, 490, 547, 627, 635
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 298
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 932
BstSLI GKGCMC 1 cut(s) 1108
BstV1I GCAGC 2 cut(s) 67, 1048
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 1 cut(s) 52
BstZ17I GTATAC 1 cut(s) 923
BsuI GTATCC 1 cut(s) 746
BsuRI GGCC 3 cut(s) 102, 273, 954
BtgI CCRYGG 3 cut(s) 217, 1003, 1034
BtsCI GGATG 6 cut(s) 213, 575, 580, 618, 638, 1080
BtsIMutI CAGTG 3 cut(s) 246, 891, 1108
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 1 cut(s) 936
CciI TCATGA 1 cut(s) 232
CseI GACGC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 529
CviAII CATG 8 cut(s) 218, 233, 295, 802, 1004, 1009, 1035, 1122
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 3 cut(s) 84, 135, 1045
DpnI GATC 5 cut(s) 54, 492, 549, 629, 637
DpnII GATC 5 cut(s) 52, 490, 547, 627, 635
EaeI YGGCCR 2 cut(s) 100, 271
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 5 cut(s) 75, 217, 274, 1003, 1034
Eco147I AGGCCT 1 cut(s) 954
Eco24I GRGCYC 1 cut(s) 1079
Eco31I GGTCTC 2 cut(s) 840, 947
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 5 cut(s) 75, 217, 274, 1003, 1034
EcoT38I GRGCYC 1 cut(s) 1079
ErhI CCWWGG 5 cut(s) 75, 217, 274, 1003, 1034
FaeI CATG 8 cut(s) 221, 236, 298, 805, 1007, 1012, 1038, 1125
FaqI GGGAC 2 cut(s) 46, 274
FatI CATG 8 cut(s) 217, 232, 294, 801, 1003, 1008, 1034, 1121
FauI CCCGC 1 cut(s) 1049
FblI GTMKAC 1 cut(s) 922
Fnu4HI GCNGC 2 cut(s) 81, 1062
FokI GGATG 6 cut(s) 200, 582, 587, 605, 625, 1067
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 2 cut(s) 81, 1062
FspBI CTAG 1 cut(s) 603
GluI GCNGC 2 cut(s) 81, 1062
GsuI CTGGAG 3 cut(s) 213, 444, 561
HaeIII GGCC 3 cut(s) 102, 273, 954
HapII CCGG 2 cut(s) 439, 975
HgaI GACGC 1 cut(s) 60
Hin1II CATG 8 cut(s) 221, 236, 298, 805, 1007, 1012, 1038, 1125
HincII GTYRAC 1 cut(s) 1098
HindII GTYRAC 1 cut(s) 1098
HinfI GANTC 4 cut(s) 467, 497, 524, 978
HpaII CCGG 2 cut(s) 439, 975
HphI GGTGA 7 cut(s) 233, 263, 313, 401, 590, 964, 1084
Hpy166II GTNNAC 3 cut(s) 9, 923, 1098
Hpy188I TCNGA 5 cut(s) 610, 627, 632, 1048, 1093
Hpy188III TCNNGA 6 cut(s) 128, 233, 385, 494, 651, 958
Hpy8I GTNNAC 3 cut(s) 9, 923, 1098
Hpy99I CGWCG 2 cut(s) 101, 627
HpyAV CCTTC 4 cut(s) 126, 168, 725, 811
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 6 cut(s) 4, 516, 659, 833, 878, 934
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 3 cut(s) 84, 135, 1045
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 8 cut(s) 221, 236, 298, 805, 1007, 1012, 1038, 1125
Kzo9I GATC 5 cut(s) 52, 490, 547, 627, 635
LmnI GCTCC 2 cut(s) 463, 1130
Lsp1109I GCAGC 2 cut(s) 67, 1048
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 87, 445, 596
MalI GATC 5 cut(s) 54, 492, 549, 629, 637
MboI GATC 5 cut(s) 52, 490, 547, 627, 635
MboII GAAGA 2 cut(s) 62, 305
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 3 cut(s) 1046, 1079, 1108
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 8 cut(s) 104, 139, 150, 264, 317, 486, 654, 863
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 2 cut(s) 461, 518
MmeI TCCRAC 4 cut(s) 605, 633, 823, 1014
MnlI CCTC 9 cut(s) 188, 294, 514, 626, 685, 758, 965, 1010, 1041
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 149, 905
MslI CAYNNNNRTG 4 cut(s) 222, 855, 1033, 1039
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 464, 1056
MspI CCGG 2 cut(s) 439, 975
MspR9I CCNGG 1 cut(s) 192
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 77
NcoI CCATGG 3 cut(s) 217, 1003, 1034
NdeII GATC 5 cut(s) 52, 490, 547, 627, 635
NlaIII CATG 8 cut(s) 221, 236, 298, 805, 1007, 1012, 1038, 1125
NlaIV GGNNCC 1 cut(s) 459
NmuCI GTSAC 3 cut(s) 87, 445, 596
NspI RCATGY 1 cut(s) 298
PagI TCATGA 1 cut(s) 232
PceI AGGCCT 1 cut(s) 954
PciI ACATGT 1 cut(s) 294
PfeI GAWTC 2 cut(s) 497, 978
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 2 cut(s) 82, 1063
PleI GAGTC 2 cut(s) 461, 518
PpsI GAGTC 2 cut(s) 461, 518
PscI ACATGT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 190
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 459
PstI CTGCAG 1 cut(s) 936
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 4 cut(s) 222, 855, 1033, 1039
SaqAI TTAA 2 cut(s) 149, 905
SatI GCNGC 2 cut(s) 81, 1062
Sau3AI GATC 5 cut(s) 52, 490, 547, 627, 635
SchI GAGTC 2 cut(s) 461, 518
ScrFI CCNGG 1 cut(s) 192
SduI GDGCHC 3 cut(s) 1046, 1079, 1108
SetI ASST 9 cut(s) 212, 466, 506, 677, 717, 726, 943, 1002, 1033
SfcI CTRYAG 1 cut(s) 932
SmiMI CAYNNNNRTG 4 cut(s) 222, 855, 1033, 1039
SmlI CTYRAG 3 cut(s) 15, 56, 956
SmoI CTYRAG 3 cut(s) 15, 56, 956
Sse9I AATT 8 cut(s) 104, 139, 150, 264, 317, 486, 654, 863
SseBI AGGCCT 1 cut(s) 954
SsiI CCGC 2 cut(s) 1014, 1056
SspI AATATT 2 cut(s) 406, 453
SspMI CTAG 1 cut(s) 603
StuI AGGCCT 1 cut(s) 954
StyD4I CCNGG 1 cut(s) 190
StyI CCWWGG 5 cut(s) 75, 217, 274, 1003, 1034
TaaI ACNGT 1 cut(s) 787
TaiI ACGT 1 cut(s) 726
TasI AATT 8 cut(s) 104, 139, 150, 264, 317, 486, 654, 863
TfiI GAWTC 2 cut(s) 497, 978
Tru1I TTAA 2 cut(s) 149, 905
Tru9I TTAA 2 cut(s) 149, 905
TscAI CASTG 3 cut(s) 253, 891, 1108
TseFI GTSAC 3 cut(s) 87, 445, 596
TseI GCWGC 2 cut(s) 80, 1061
Tsp45I GTSAC 3 cut(s) 87, 445, 596
TspDTI ATGAA 7 cut(s) 97, 202, 221, 334, 760, 790, 1110
TspGWI ACGGA 3 cut(s) 611, 829, 1128
TspRI CASTG 3 cut(s) 253, 891, 1108
Van91I CCANNNNNTGG 1 cut(s) 650
XapI RAATTY 1 cut(s) 863
XceI RCATGY 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 922
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.