RLG00000015306
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004361
Physical Location & Seq
Forward (+)
64286964 .. 64290350
3387 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015306

Sequence Viewer

Length: 1224 bp
ATGCCTTCTGCTTTCTGGTTCTTCCTTTCGATTTTCCTATGCCTCAGCTACGTCTCTACGGCGGCGGAGCCGGTGACCTGCTCAGGGATAGTGCCAATGAAGAGAGGAGGCACGCTTCTTTACATACCTCCTGGGGTGTACTTAACGGAGAGCTTCAATCTCACTAGCCACATGACTCTCTACTTGGCTAGAGGTGCTGTTATCAAAGCCACACAGGACACTAAGAATTGGCGTTTAATTGCTCCTTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGCGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTTACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATTGGATTGCTTATGGTCACCCTAGTTCCGACATTACCGTCCGTCGGATCAGAGGATCATCCCCATTTTCTGGAATTGCAATAGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACGCATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAACATCGGTAGGGGAGGCTTCATAAGAAACATAACAGTTTCAAATGTGTTCATGGAAAAGGCACGGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAGGGGTATCACATTAACAGACATCTGGGGTATACAAGTTCTGCAGGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCGGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACTGCAACAACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTTGGAGTTGACCAGTGCCCAACACGCAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

408

Amino Acids

44.47

Weight (kDa)

7.16

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 99 - 380 3e-40 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
Acc36I ACCTGC 2 cut(s) 86, 1016
AccB7I CCANNNNNTGG 2 cut(s) 737, 1177
AccI GTMKAC 1 cut(s) 1009
AciI CCGC 3 cut(s) 62, 65, 1143
AclWI GGATC 3 cut(s) 572, 722, 730
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 1 cut(s) 950
AfaI GTAC 2 cut(s) 140, 617
AfiI CCNNNNNNNGG 6 cut(s) 84, 257, 711, 737, 1061, 1177
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 5 cut(s) 157, 404, 588, 879, 955
AjnI CCWGG 2 cut(s) 130, 277
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 5 cut(s) 48, 153, 297, 551, 591
AluI AGCT 5 cut(s) 48, 153, 297, 551, 591
Alw21I GWGCWC 1 cut(s) 1133
Alw26I GTCTC 3 cut(s) 58, 927, 1034
AlwI GGATC 3 cut(s) 572, 722, 730
AoxI GGCC 2 cut(s) 358, 1039
ApeKI GCWGC 1 cut(s) 1148
ApoI RAATTY 1 cut(s) 950
AsuHPI GGTGA 7 cut(s) 85, 350, 400, 488, 677, 1051, 1171
BaeGI GKGCMC 1 cut(s) 1195
BalI TGGCCA 1 cut(s) 360
BanII GRGCYC 1 cut(s) 1166
Bbv12I GWGCWC 1 cut(s) 1133
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 1135
BccI CCATC 5 cut(s) 305, 350, 563, 651, 1175
BceAI ACGGC 2 cut(s) 75, 235
BciT130I CCWGG 2 cut(s) 132, 279
BciVI GTATCC 1 cut(s) 833
BcoDI GTCTC 3 cut(s) 58, 927, 1034
BfaI CTAG 3 cut(s) 165, 189, 690
BfmI CTRYAG 1 cut(s) 1019
BfuAI ACCTGC 2 cut(s) 86, 1016
BfuI GTATCC 1 cut(s) 833
BisI GCNGC 2 cut(s) 63, 1149
BlsI GCNGC 2 cut(s) 64, 1150
Bme1390I CCNGG 2 cut(s) 132, 279
BmiI GGNNCC 2 cut(s) 69, 546
BmrFI CCNGG 2 cut(s) 132, 279
BmrI ACTGGG 1 cut(s) 965
BmuI ACTGGG 1 cut(s) 965
BpmI CTGGAG 3 cut(s) 300, 531, 648
Bpu10I CCTNAGC 2 cut(s) 44, 82
BpuEI CTTGAG 1 cut(s) 1028
BsaI GGTCTC 2 cut(s) 927, 1034
BsaJI CCNNGG 6 cut(s) 131, 277, 304, 361, 1090, 1121
BsaWI WCCGGW 2 cut(s) 525, 1061
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 257, 711, 737, 1061, 1177
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 4 cut(s) 340, 631, 971, 1188
BseBI CCWGG 2 cut(s) 132, 279
BseDI CCNNGG 6 cut(s) 131, 277, 304, 361, 1090, 1121
BseGI GGATG 5 cut(s) 300, 662, 667, 725, 1167
BseLI CCNNNNNNNGG 6 cut(s) 84, 257, 711, 737, 1061, 1177
BseMII CTCAG 3 cut(s) 58, 96, 1146
BseNI ACTGG 4 cut(s) 340, 631, 971, 1188
BseRI GAGGAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 1195
BseXI GCAGC 1 cut(s) 1135
BshFI GGCC 2 cut(s) 360, 1041
BsiHKAI GWGCWC 1 cut(s) 1133
BsiSI CCGG 3 cut(s) 71, 526, 1062
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 6 cut(s) 84, 257, 711, 737, 1061, 1177
BsmAI GTCTC 3 cut(s) 58, 927, 1034
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 2 cut(s) 360, 1041
Bso31I GGTCTC 2 cut(s) 927, 1034
Bsp1286I GDGCHC 3 cut(s) 1133, 1166, 1195
Bsp143I GATC 4 cut(s) 577, 634, 714, 722
Bsp19I CCATGG 3 cut(s) 304, 1090, 1121
BspACI CCGC 3 cut(s) 62, 65, 1143
BspANI GGCC 2 cut(s) 360, 1041
BspCNI CTCAG 3 cut(s) 57, 95, 1145
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 69, 546
BspMAI CTGCAG 1 cut(s) 1023
BspMI ACCTGC 2 cut(s) 86, 1016
BspPI GGATC 3 cut(s) 572, 722, 730
BspTNI GGTCTC 2 cut(s) 927, 1034
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 4 cut(s) 340, 631, 971, 1188
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 6 cut(s) 131, 277, 304, 361, 1090, 1121
BssMI GATC 4 cut(s) 577, 634, 714, 722
BssNAI GTATAC 1 cut(s) 1010
BssT1I CCWWGG 4 cut(s) 304, 361, 1090, 1121
Bst1107I GTATAC 1 cut(s) 1010
Bst2UI CCWGG 2 cut(s) 132, 279
Bst4CI ACNGT 2 cut(s) 706, 874
Bst6I CTCTTC 2 cut(s) 95, 405
BstC8I GCNNGC 2 cut(s) 113, 1023
BstDEI CTNAG 4 cut(s) 44, 82, 222, 1132
BstDSI CCRYGG 3 cut(s) 304, 1090, 1121
BstEII GGTNACC 2 cut(s) 73, 683
BstF5I GGATG 5 cut(s) 300, 662, 667, 725, 1167
BstKTI GATC 4 cut(s) 580, 637, 717, 725
BstMAI GTCTC 3 cut(s) 58, 927, 1034
BstMBI GATC 4 cut(s) 577, 634, 714, 722
BstMWI GCNNNNNNNGC 2 cut(s) 194, 1199
BstNI CCWGG 2 cut(s) 132, 279
BstNSI RCATGY 1 cut(s) 385
BstPI GGTNACC 2 cut(s) 73, 683
BstSCI CCNGG 2 cut(s) 130, 277
BstSFI CTRYAG 1 cut(s) 1019
BstSLI GKGCMC 1 cut(s) 1195
BstV1I GCAGC 1 cut(s) 1135
BstXI CCANNNNNNTGG 1 cut(s) 311
BstZ17I GTATAC 1 cut(s) 1010
BsuI GTATCC 1 cut(s) 833
BsuRI GGCC 2 cut(s) 360, 1041
BtgI CCRYGG 3 cut(s) 304, 1090, 1121
BtgZI GCGATG 1 cut(s) 324
BtsCI GGATG 5 cut(s) 300, 662, 667, 725, 1167
BtsIMutI CAGTG 3 cut(s) 333, 978, 1195
BveI ACCTGC 2 cut(s) 86, 1016
Cac8I GCNNGC 2 cut(s) 113, 1023
CciI TCATGA 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 70
Csp6I GTAC 2 cut(s) 139, 616
CviAII CATG 9 cut(s) 172, 305, 320, 382, 889, 1091, 1096, 1122, 1209
CviQI GTAC 2 cut(s) 139, 616
DdeI CTNAG 4 cut(s) 44, 82, 222, 1132
DpnI GATC 4 cut(s) 579, 636, 716, 724
DpnII GATC 4 cut(s) 577, 634, 714, 722
DrdI GACNNNNNNGTC 1 cut(s) 704
DseDI GACNNNNNNGTC 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 95, 405
EarI CTCTTC 2 cut(s) 95, 405
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 4 cut(s) 304, 361, 1090, 1121
Eco147I AGGCCT 1 cut(s) 1041
Eco24I GRGCYC 1 cut(s) 1166
Eco31I GGTCTC 2 cut(s) 927, 1034
Eco91I GGTNACC 2 cut(s) 73, 683
EcoO65I GGTNACC 2 cut(s) 73, 683
EcoRII CCWGG 2 cut(s) 130, 277
EcoT14I CCWWGG 4 cut(s) 304, 361, 1090, 1121
EcoT38I GRGCYC 1 cut(s) 1166
ErhI CCWWGG 4 cut(s) 304, 361, 1090, 1121
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 9 cut(s) 175, 308, 323, 385, 892, 1094, 1099, 1125, 1212
FaqI GGGAC 1 cut(s) 361
FatI CATG 9 cut(s) 171, 304, 319, 381, 888, 1090, 1095, 1121, 1208
FauI CCCGC 1 cut(s) 1136
FblI GTMKAC 1 cut(s) 1009
Fnu4HI GCNGC 2 cut(s) 63, 1149
FokI GGATG 5 cut(s) 287, 669, 674, 712, 1154
FriOI GRGCYC 1 cut(s) 1166
Fsp4HI GCNGC 2 cut(s) 63, 1149
FspBI CTAG 3 cut(s) 165, 189, 690
GluI GCNGC 2 cut(s) 63, 1149
GsuI CTGGAG 3 cut(s) 300, 531, 648
HaeIII GGCC 2 cut(s) 360, 1041
HapII CCGG 3 cut(s) 71, 526, 1062
Hin1II CATG 9 cut(s) 175, 308, 323, 385, 892, 1094, 1099, 1125, 1212
HincII GTYRAC 1 cut(s) 1185
HindII GTYRAC 1 cut(s) 1185
HinfI GANTC 5 cut(s) 175, 554, 584, 611, 1065
HpaII CCGG 3 cut(s) 71, 526, 1062
HphI GGTGA 7 cut(s) 85, 350, 400, 488, 677, 1051, 1171
Hpy166II GTNNAC 3 cut(s) 139, 1010, 1185
Hpy188I TCNGA 4 cut(s) 697, 714, 719, 1135
Hpy188III TCNNGA 5 cut(s) 320, 472, 581, 738, 1045
Hpy8I GTNNAC 3 cut(s) 139, 1010, 1185
Hpy99I CGWCG 1 cut(s) 714
HpyAV CCTTC 4 cut(s) 15, 255, 812, 898
HpyCH4III ACNGT 2 cut(s) 706, 874
HpyCH4IV ACGT 2 cut(s) 51, 810
HpyCH4V TGCA 6 cut(s) 603, 746, 920, 965, 1021, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 1199
HpyF3I CTNAG 4 cut(s) 44, 82, 222, 1132
HpySE526I ACGT 2 cut(s) 51, 810
Hsp92II CATG 9 cut(s) 175, 308, 323, 385, 892, 1094, 1099, 1125, 1212
Kzo9I GATC 4 cut(s) 577, 634, 714, 722
LmnI GCTCC 4 cut(s) 67, 247, 550, 1217
Lsp1109I GCAGC 1 cut(s) 1135
MaeI CTAG 3 cut(s) 165, 189, 690
MaeII ACGT 2 cut(s) 51, 810
MaeIII GTNAC 3 cut(s) 73, 532, 683
MalI GATC 4 cut(s) 579, 636, 716, 724
MboI GATC 4 cut(s) 577, 634, 714, 722
MboII GAAGA 3 cut(s) 13, 112, 392
MfeI CAATTG 1 cut(s) 351
MhlI GDGCHC 3 cut(s) 1133, 1166, 1195
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 7 cut(s) 226, 237, 351, 404, 573, 741, 950
MluNI TGGCCA 1 cut(s) 360
MlyI GAGTC 3 cut(s) 169, 548, 605
MmeI TCCRAC 4 cut(s) 692, 720, 910, 1101
Mox20I TGGCCA 1 cut(s) 360
MscI TGGCCA 1 cut(s) 360
MseI TTAA 3 cut(s) 143, 236, 992
MslI CAYNNNNRTG 4 cut(s) 309, 942, 1120, 1126
Msp20I TGGCCA 1 cut(s) 360
MspA1I CMGCKG 2 cut(s) 551, 1143
MspI CCGG 3 cut(s) 71, 526, 1062
MspR9I CCNGG 2 cut(s) 132, 279
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 2 cut(s) 132, 279
MwoI GCNNNNNNNGC 2 cut(s) 194, 1199
NcoI CCATGG 3 cut(s) 304, 1090, 1121
NdeII GATC 4 cut(s) 577, 634, 714, 722
NlaIII CATG 9 cut(s) 175, 308, 323, 385, 892, 1094, 1099, 1125, 1212
NlaIV GGNNCC 2 cut(s) 69, 546
NmuCI GTSAC 2 cut(s) 73, 683
NspI RCATGY 1 cut(s) 385
PagI TCATGA 1 cut(s) 319
PceI AGGCCT 1 cut(s) 1041
PciI ACATGT 1 cut(s) 381
PfeI GAWTC 2 cut(s) 584, 1065
PflMI CCANNNNNTGG 2 cut(s) 737, 1177
PkrI GCNGC 2 cut(s) 64, 1150
PleI GAGTC 3 cut(s) 169, 548, 605
PpsI GAGTC 3 cut(s) 169, 548, 605
PscI ACATGT 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 130, 277
PspEI GGTNACC 2 cut(s) 73, 683
PspGI CCWGG 2 cut(s) 130, 277
PspN4I GGNNCC 2 cut(s) 69, 546
PstI CTGCAG 1 cut(s) 1023
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 140, 617
RsaNI GTAC 2 cut(s) 139, 616
RseI CAYNNNNRTG 4 cut(s) 309, 942, 1120, 1126
SaqAI TTAA 3 cut(s) 143, 236, 992
SatI GCNGC 2 cut(s) 63, 1149
Sau3AI GATC 4 cut(s) 577, 634, 714, 722
SchI GAGTC 3 cut(s) 169, 548, 605
ScrFI CCNGG 2 cut(s) 132, 279
SduI GDGCHC 3 cut(s) 1133, 1166, 1195
SfcI CTRYAG 1 cut(s) 1019
SmiMI CAYNNNNRTG 4 cut(s) 309, 942, 1120, 1126
SmlI CTYRAG 1 cut(s) 1043
SmoI CTYRAG 1 cut(s) 1043
Sse9I AATT 7 cut(s) 226, 237, 351, 404, 573, 741, 950
SseBI AGGCCT 1 cut(s) 1041
SsiI CCGC 3 cut(s) 62, 65, 1143
SspI AATATT 2 cut(s) 493, 540
SspMI CTAG 3 cut(s) 165, 189, 690
StuI AGGCCT 1 cut(s) 1041
StyD4I CCNGG 2 cut(s) 130, 277
StyI CCWWGG 4 cut(s) 304, 361, 1090, 1121
TaaI ACNGT 2 cut(s) 706, 874
TaiI ACGT 2 cut(s) 54, 813
TaqI TCGA 1 cut(s) 29
TasI AATT 7 cut(s) 226, 237, 351, 404, 573, 741, 950
TatI WGTACW 1 cut(s) 138
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 584, 1065
Tru1I TTAA 3 cut(s) 143, 236, 992
Tru9I TTAA 3 cut(s) 143, 236, 992
TscAI CASTG 3 cut(s) 340, 978, 1195
TseFI GTSAC 2 cut(s) 73, 683
TseI GCWGC 1 cut(s) 1148
Tsp45I GTSAC 2 cut(s) 73, 683
TspDTI ATGAA 7 cut(s) 113, 289, 308, 421, 847, 877, 1197
TspGWI ACGGA 3 cut(s) 161, 698, 916
TspRI CASTG 3 cut(s) 340, 978, 1195
Van91I CCANNNNNTGG 2 cut(s) 737, 1177
XapI RAATTY 1 cut(s) 950
XceI RCATGY 1 cut(s) 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 1174
XmiI GTMKAC 1 cut(s) 1009
XspI CTAG 3 cut(s) 165, 189, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.