Rh6CG024900
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004361
Physical Location & Seq
Reverse (-)
2279373 .. 2282162
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG024900.1

Sequence Viewer

Length: 1035 bp
ATGGGCAAAGGTGGATTTGCAACTATTGACACTAAGAATTGGCGTTTAATTGCTCCTTTGCCGTCTTATGGAAGGGGGAGAGAACGCCCTGGAGGGAGATATGTGAGCTTCATCCATGGCGATGGAGTTCATGATGTGGTTATCACTGGTGAGAATGGGACAATTGATGGCCAAGGAGATGTGTGGTGGAACATGTGGAGGCAAAGAACTCTTCAATTCACCAGACCAAATCTCATTGAGTTCATCAACTCTAAAAACATTATCATTTCCAATGTGATTTTCCAGAACTCTCCATTTTGGAATATTCACCCTGTTTATTCCAGCAACATTGTTGTCCGGTATGTCACAATATTGGCTCCAGCTGACTCTCCCAACACCGATGGAATTGATCCAGATTCAAGCTCTAATGTCTGCATAGAGGACTCGTACATATCTACTGGAGATGATCTTGTGGCTGTAAAAAGTGGATGGGATGAGTATGGGATTGCTTATGGTCACCCTAGTTCCGACATTACCATCCGTCGGATCAGAGGATCATCCCCATTTTCTGGAATTGCAATAGGAAGTGAAACCTCTGGTGGAGTGGAGAATGTATTTGCCGAACACATAAACCTTCACAACGTAGGAGTTGGTATCCATATAAAAACAAACATCGGTAGGGGAGGCTTCATAAGAAACATAACAGTTTCAAATGTGTTCATGGAAAAGGCACGGAAGGGTATAAAGATTGCAGGAGATGTTGGAGACCACCCTGATGACAAATTCAATCCAAATGCACTCCCAGTGGTAAAGGGTATCACATTAACAGACATCTGGGGTATACAAGTTCTGCAGGCAGGTCTCATACAAGGCCTCAAGAACTCCCCTTTCACCGGAATCTGTCTTTCCAACATCAACCTCCATGGCATGACCGCAACAACAAGAACACCTCCATGGAAGTGCTCAGATGTCAGCGGGGCTGCTCATCTGGTGAGCCCCATCCAATGCTCGGAGTTGACCAGTGCCCAACACACAACTTCATGCTCCAACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

344

Amino Acids

37.32

Weight (kDa)

6.59

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 36 - 317 5.8e-41 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 1 cut(s) 820
AciI CCGC 2 cut(s) 912, 954
AclWI GGATC 3 cut(s) 383, 533, 541
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 761
AfaI GTAC 1 cut(s) 428
AfiI CCNNNNNNNGG 5 cut(s) 68, 522, 548, 872, 988
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 4 cut(s) 215, 399, 690, 766
AjnI CCWGG 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 3 cut(s) 108, 362, 402
AluI AGCT 3 cut(s) 108, 362, 402
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 2 cut(s) 738, 845
AlwI GGATC 3 cut(s) 383, 533, 541
AoxI GGCC 2 cut(s) 169, 850
ApeKI GCWGC 1 cut(s) 959
ApoI RAATTY 1 cut(s) 761
AsuHPI GGTGA 6 cut(s) 161, 211, 299, 488, 862, 982
BaeGI GKGCMC 1 cut(s) 1006
BalI TGGCCA 1 cut(s) 171
BanII GRGCYC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 944
BbvI GCAGC 1 cut(s) 946
BccI CCATC 6 cut(s) 116, 161, 374, 462, 524, 986
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 90
BciVI GTATCC 1 cut(s) 644
BcoDI GTCTC 2 cut(s) 738, 845
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 830
BfuAI ACCTGC 1 cut(s) 827
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 1 cut(s) 960
BlsI GCNGC 1 cut(s) 961
Bme1390I CCNGG 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 776
BmuI ACTGGG 1 cut(s) 776
BpmI CTGGAG 3 cut(s) 111, 342, 459
BpuEI CTTGAG 1 cut(s) 839
BsaI GGTCTC 2 cut(s) 738, 845
BsaJI CCNNGG 5 cut(s) 88, 115, 172, 901, 932
BsaWI WCCGGW 2 cut(s) 336, 872
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 522, 548, 872, 988
Bse1I ACTGG 4 cut(s) 151, 442, 782, 999
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 5 cut(s) 88, 115, 172, 901, 932
BseGI GGATG 6 cut(s) 111, 473, 478, 516, 536, 978
BseLI CCNNNNNNNGG 5 cut(s) 68, 522, 548, 872, 988
BseMII CTCAG 1 cut(s) 957
BseNI ACTGG 4 cut(s) 151, 442, 782, 999
BseSI GKGCMC 1 cut(s) 1006
BseXI GCAGC 1 cut(s) 946
BshFI GGCC 2 cut(s) 171, 852
BsiHKAI GWGCWC 1 cut(s) 944
BsiSI CCGG 2 cut(s) 337, 873
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 5 cut(s) 68, 522, 548, 872, 988
BsmAI GTCTC 2 cut(s) 738, 845
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 2 cut(s) 171, 852
Bso31I GGTCTC 2 cut(s) 738, 845
Bsp1286I GDGCHC 3 cut(s) 944, 977, 1006
Bsp143I GATC 4 cut(s) 388, 445, 525, 533
Bsp19I CCATGG 3 cut(s) 115, 901, 932
BspACI CCGC 2 cut(s) 912, 954
BspANI GGCC 2 cut(s) 171, 852
BspCNI CTCAG 1 cut(s) 956
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 357
BspMAI CTGCAG 1 cut(s) 834
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 3 cut(s) 383, 533, 541
BspTNI GGTCTC 2 cut(s) 738, 845
BsrI ACTGG 4 cut(s) 151, 442, 782, 999
BssECI CCNNGG 5 cut(s) 88, 115, 172, 901, 932
BssMI GATC 4 cut(s) 388, 445, 525, 533
BssNAI GTATAC 1 cut(s) 821
BssT1I CCWWGG 4 cut(s) 115, 172, 901, 932
Bst1107I GTATAC 1 cut(s) 821
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 2 cut(s) 33, 943
BstDSI CCRYGG 3 cut(s) 115, 901, 932
BstEII GGTNACC 1 cut(s) 494
BstF5I GGATG 6 cut(s) 111, 473, 478, 516, 536, 978
BstKTI GATC 4 cut(s) 391, 448, 528, 536
BstMAI GTCTC 2 cut(s) 738, 845
BstMBI GATC 4 cut(s) 388, 445, 525, 533
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 196
BstPI GGTNACC 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 1 cut(s) 830
BstSLI GKGCMC 1 cut(s) 1006
BstV1I GCAGC 1 cut(s) 946
BstXI CCANNNNNNTGG 1 cut(s) 122
BstZ17I GTATAC 1 cut(s) 821
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 2 cut(s) 171, 852
BtgI CCRYGG 3 cut(s) 115, 901, 932
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 6 cut(s) 111, 473, 478, 516, 536, 978
BtsIMutI CAGTG 3 cut(s) 144, 789, 1006
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 1 cut(s) 834
CciI TCATGA 1 cut(s) 130
Csp6I GTAC 1 cut(s) 427
CviAII CATG 8 cut(s) 116, 131, 193, 700, 902, 907, 933, 1020
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 2 cut(s) 33, 943
DpnI GATC 4 cut(s) 390, 447, 527, 535
DpnII GATC 4 cut(s) 388, 445, 525, 533
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 4 cut(s) 115, 172, 901, 932
Eco147I AGGCCT 1 cut(s) 852
Eco24I GRGCYC 1 cut(s) 977
Eco31I GGTCTC 2 cut(s) 738, 845
Eco91I GGTNACC 1 cut(s) 494
EcoO65I GGTNACC 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 4 cut(s) 115, 172, 901, 932
EcoT38I GRGCYC 1 cut(s) 977
ErhI CCWWGG 4 cut(s) 115, 172, 901, 932
FaeI CATG 8 cut(s) 119, 134, 196, 703, 905, 910, 936, 1023
FaqI GGGAC 1 cut(s) 172
FatI CATG 8 cut(s) 115, 130, 192, 699, 901, 906, 932, 1019
FauI CCCGC 1 cut(s) 947
FblI GTMKAC 1 cut(s) 820
Fnu4HI GCNGC 1 cut(s) 960
FokI GGATG 6 cut(s) 98, 480, 485, 503, 523, 965
FriOI GRGCYC 1 cut(s) 977
Fsp4HI GCNGC 1 cut(s) 960
FspBI CTAG 1 cut(s) 501
GluI GCNGC 1 cut(s) 960
GsuI CTGGAG 3 cut(s) 111, 342, 459
HaeIII GGCC 2 cut(s) 171, 852
HapII CCGG 2 cut(s) 337, 873
Hin1II CATG 8 cut(s) 119, 134, 196, 703, 905, 910, 936, 1023
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HinfI GANTC 4 cut(s) 365, 395, 422, 876
HpaII CCGG 2 cut(s) 337, 873
HphI GGTGA 6 cut(s) 161, 211, 299, 488, 862, 982
Hpy166II GTNNAC 2 cut(s) 821, 996
Hpy188I TCNGA 5 cut(s) 508, 525, 530, 946, 991
Hpy188III TCNNGA 5 cut(s) 131, 283, 392, 549, 856
Hpy8I GTNNAC 2 cut(s) 821, 996
Hpy99I CGWCG 1 cut(s) 525
HpyAV CCTTC 3 cut(s) 66, 623, 709
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 6 cut(s) 20, 414, 557, 731, 776, 832
HpyF3I CTNAG 2 cut(s) 33, 943
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 8 cut(s) 119, 134, 196, 703, 905, 910, 936, 1023
Kzo9I GATC 4 cut(s) 388, 445, 525, 533
LmnI GCTCC 3 cut(s) 58, 361, 1028
Lsp1109I GCAGC 1 cut(s) 946
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 343, 494
MalI GATC 4 cut(s) 390, 447, 527, 535
MboI GATC 4 cut(s) 388, 445, 525, 533
MboII GAAGA 1 cut(s) 203
MfeI CAATTG 1 cut(s) 162
MhlI GDGCHC 3 cut(s) 944, 977, 1006
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 7 cut(s) 37, 48, 162, 215, 384, 552, 761
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 2 cut(s) 359, 416
MmeI TCCRAC 4 cut(s) 503, 531, 721, 912
MnlI CCTC 9 cut(s) 86, 192, 412, 524, 583, 656, 863, 908, 939
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MseI TTAA 2 cut(s) 47, 803
MslI CAYNNNNRTG 4 cut(s) 120, 753, 931, 937
Msp20I TGGCCA 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 362, 954
MspI CCGG 2 cut(s) 337, 873
MspR9I CCNGG 1 cut(s) 90
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 90
NcoI CCATGG 3 cut(s) 115, 901, 932
NdeII GATC 4 cut(s) 388, 445, 525, 533
NlaIII CATG 8 cut(s) 119, 134, 196, 703, 905, 910, 936, 1023
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 343, 494
NspI RCATGY 1 cut(s) 196
PagI TCATGA 1 cut(s) 130
PceI AGGCCT 1 cut(s) 852
PciI ACATGT 1 cut(s) 192
PfeI GAWTC 2 cut(s) 395, 876
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 1 cut(s) 961
PleI GAGTC 2 cut(s) 359, 416
PpsI GAGTC 2 cut(s) 359, 416
PscI ACATGT 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 88
PspEI GGTNACC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
RseI CAYNNNNRTG 4 cut(s) 120, 753, 931, 937
SaqAI TTAA 2 cut(s) 47, 803
SatI GCNGC 1 cut(s) 960
Sau3AI GATC 4 cut(s) 388, 445, 525, 533
SchI GAGTC 2 cut(s) 359, 416
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 3 cut(s) 944, 977, 1006
SfcI CTRYAG 1 cut(s) 830
SmiMI CAYNNNNRTG 4 cut(s) 120, 753, 931, 937
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 7 cut(s) 37, 48, 162, 215, 384, 552, 761
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 2 cut(s) 912, 954
SspI AATATT 2 cut(s) 304, 351
SspMI CTAG 1 cut(s) 501
StuI AGGCCT 1 cut(s) 852
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 4 cut(s) 115, 172, 901, 932
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 1 cut(s) 624
TasI AATT 7 cut(s) 37, 48, 162, 215, 384, 552, 761
TfiI GAWTC 2 cut(s) 395, 876
Tru1I TTAA 2 cut(s) 47, 803
Tru9I TTAA 2 cut(s) 47, 803
TscAI CASTG 3 cut(s) 151, 789, 1006
TseFI GTSAC 2 cut(s) 343, 494
TseI GCWGC 1 cut(s) 959
Tsp45I GTSAC 2 cut(s) 343, 494
TspDTI ATGAA 6 cut(s) 100, 119, 232, 658, 688, 1008
TspGWI ACGGA 2 cut(s) 509, 727
TspRI CASTG 3 cut(s) 151, 789, 1006
Van91I CCANNNNNTGG 1 cut(s) 548
XapI RAATTY 1 cut(s) 761
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 820
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.