Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004613

Physical Location & Seq

Genomic Coordinates Reverse (-)

30271183 .. 30275113

Gene Structure 3931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1221000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGACGCGCGTCGCGTCTCCCGTACCGTGATCGACCCCAAGGTCCGCCAAGTCGGGTTCTTCACCCCCGTAGCCGAAGCCCCACCGTGTCGGACCCAGTCCGGCCCGGCAATCACAGCCCCTTCCCCTCTCTCCGAATCTCCGGCCAGCAACTCTCTCTCTCCCGTCATGATCCCCCCGTCGCGTCACCTCTCCGACACAGTCGTGATCCGTACGGCAGCCGTGCCGGTACCGGATTCGGCGTTGCGGCGCACGGAATCCGGCGACGTGCCGGTGGGCAGCTATAATCCCTTGGAGTCGTTGTTAGGGACCTCGCCGGTGTCAGCCTCGCCTTCGAGTCGCGTTGGGGATGGACTCGAGTTCTCCGATGAGTCCTCGGCCCGTTGGAATCGGCAGAGCGGCTCCGGAAAGATGGCCTCGAGCTTGCCCGGCGTAGGATTCGAATCCGCAGCATTGAAGACGGCTGCTGAAGTTCCTGAAAAGAGTGGAGAGGCGGTTGCCGAATCGCAGAAAGAACTTCCGGCAAATTCAAAACCTTTGACAGCCAAGACCACAAAAGCGGAAAGGCGTGCCTTGCAAGAGGCTCAACGCGCTGCAAAAGCCGCTGCTAGAGGTGAGGGTAAAAAACCTGCTGCTGTAACTGGAGGTGGCGCTACTGGTAAGGCAGCTAAGTCTGCAAAGCAGTCTGTGCAAAAGAAGGATACTTCTCAAGTTGCGCCTTCGGTGGCTGCTTCTGATAGAAAGGGGGGTGATCGACCACCAGATAAGGAAAGGAAGAAAGATGTTCCTCCTCCACGTATGCAGTTTGATGATAAGAGCCGAGTGGAGAAGGCAAAAAGGCGTGCAGTGGTCCATCAAACAGAAGCTAAAAACAGAGTTGAATTGTTCCGGCATTTGCCTCAATACGAGCGTGGAACTCAGCTTCCTGATCTTGAATCAAAGTTTTTCCAACTTGATCCAATGCATCCTGCCATTTATAAGGTTGGACTGAAGTATTTGGCTGGAGAAGTATCAGGGGGTAATGCTCGTTGTATTGCAATGCTCCAAGCATTTCAAGAAGCCATTAGAGACTACTCAACACCGCCAAAGAAATTTCTTGTCAGAGATTTAACTGCAAAACTCAGTAGTTATGTCTCATTTTTCACTGAATGCAGGCCACTTTCGATCAGCATGGGAAATGCAGTAAGGTTTCTGAAGAGTCGTATTGCCAAGCTCCCCTTAAATCTTTCCGAGTCTGACGCAAAATCTACACTTTGTTCAGAAATTGACCGTTTTATAAATGAAAAGATAATACTTGCTGATAAGGTGATAGTCGGGCATGCTTCTACAAAGGTTAGGGACGGAGATGTTCTTCTCACGTACGGATCATCATGTGTTGTTGAGATGATTCTGCTATATGCCCATGAACTCGGAAAAAAGTTTCGTGTTGTGGTAGTAGACTCCCGTCCAAAGCTTGAAGGCCAAGCCTTACTTCGTAGGCTGGTCGCAAAGGGCCTGAGTTGTACATATACTCATATAAACGCCGTGTCTTATATCATGCATGAAGTAACGCGAGTGTTTTTGGGAGCCGCCTCAGTATTATCTAATGGAACTGTATATTCGAGAGTGGGAACTGCATGTGTTGCTATGGTTGCTCATGCATTTTGTGTTCCAGTCTTGATCTGCTGTGAAGCTTATAAGTTTCACGAAAGGGTACAGCTTGATTCAATTTGCTCTAACGAACTAGGTGATCCAAATGCTATTGCGAAGGTTTCTGGAAGAACGGATGTAAACTATCTAGATAATTGGGCGAACAAGGAAAATCTGCAACTTCTGAATTTAATGTATGATGCTACTCCGTCAGATTATATATCTATGATTGTCACAGATTACGGCATGATTCCACCCACAAGCGTGCCGGTCATTGTCCGCGAATATGGGAGAGAGCACTTGTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0002253 GO:0002376 GO:0002429 GO:0002682 GO:0002684 GO:0002757 GO:0002764 GO:0002768 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007272 GO:0007275 GO:0007399 GO:0007417 GO:0007548 GO:0008150 GO:0008152 GO:0008366 GO:0008406 GO:0008585 GO:0009058 GO:0009059 GO:0009987 GO:0010001 GO:0010467 GO:0014003 GO:0019538 GO:0021782 GO:0022008 GO:0022414 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0034645 GO:0042063 GO:0042552 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048468 GO:0048513 GO:0048518 GO:0048583 GO:0048584 GO:0048608 GO:0048709 GO:0048731 GO:0048856 GO:0048869 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050851 GO:0050852 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

IF-2B

Protein Analysis

646

Amino Acids

69.99

Weight (kDa)

9.32

Isoelectric Point (pI)

46.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IF-2B	PF01008	336 - 629	6e-76	Initiation factor 2 subunit family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48970 AT1G48970
fragaria_vesca	FvH4_3g05690 FvH4_5g12800 FvH4_5g12800
malus_domestica	MD06G1210100.v1.1 MD14G1221000.v1.1
prunus_persica	Prupe.5G216300_v2.0.a1
pyrus_communis	pycom06g18780 pycom14g18290
rosa_chinensis	RchiOBHm_Chr4g0426391 RchiOBHm_Chr7g0184441
rosa_laevigata	RLG00000004975 RLG00000007298
rosa_multiflora	Rmu_sc0001557.1_g000016 Rmu_sc0006158.1_g000003 Rmu_sc0006158.1_g000004 Rmu_sc0007942.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269610 Rroxscaffold_5G00368400
rosa_rugosa	Rorug04G0210500
rosa_samantha	Rh3DG253600 Rh4BG273600 Rh4CG287800 Rh4CG287900 Rh4DG270400 Rh7AG079900 Rh7BG067300 Rh7CG067600 Rh7DG067300
rosa_wichuraiana	Rw7G005540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	978, 1277, 1677
AasI	GACNNNNNNGTC	1 cut(s)	41
Acc36I	ACCTGC	1 cut(s)	637
Acc65I	GGTACC	1 cut(s)	229
AccB1I	GGYRCC	1 cut(s)	229
AccBSI	CCGCTC	1 cut(s)	399
AccI	GTMKAC	1 cut(s)	1437
AccII	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
AccIII	TCCGGA	1 cut(s)	404
AclWI	GGATC	5 cut(s)	166, 202, 950, 1372, 1724
AcoI	YGGCCR	1 cut(s)	144
AcsI	RAATTY	3 cut(s)	526, 1091, 1816
AcuI	CTGAAG	3 cut(s)	489, 1010, 1214
AfaI	GTAC	6 cut(s)	25, 214, 231, 1361, 1504, 1695
AfiI	CCNNNNNNNGG	1 cut(s)	434
AgsI	TTSAA	7 cut(s)	457, 531, 881, 935, 1055, 1457, 1707
AjiI	CACGTC	1 cut(s)	268
AjuI	GAANNNNNNNTTGG	2 cut(s)	1455, 1487
AleI	CACNNNNGTG	2 cut(s)	203, 1892
AloI	GAACNNNNNNTCC	2 cut(s)	907, 939
AluBI	AGCT	9 cut(s)	282, 423, 668, 866, 922, 1213, 1453, 1673, 1699
AluI	AGCT	9 cut(s)	282, 423, 668, 866, 922, 1213, 1453, 1673, 1699
Alw21I	GWGCWC	1 cut(s)	1929
Alw26I	GTCTC	3 cut(s)	22, 1062, 1138
AlwI	GGATC	5 cut(s)	166, 202, 950, 1372, 1724
Ama87I	CYCGRG	2 cut(s)	356, 418
Aor13HI	TCCGGA	1 cut(s)	404
AoxI	GGCC	7 cut(s)	103, 144, 378, 414, 1154, 1459, 1492
ApeKI	GCWGC	9 cut(s)	218, 279, 449, 464, 593, 605, 632, 665, 728
ApoI	RAATTY	3 cut(s)	526, 1091, 1816
Asp718I	GGTACC	1 cut(s)	229
AspLEI	GCGC	5 cut(s)	10, 252, 593, 653, 718
AspS9I	GGNCC	7 cut(s)	43, 93, 104, 309, 379, 850, 1492
AsuC2I	CCSGG	2 cut(s)	107, 429
AsuHPI	GGTGA	6 cut(s)	55, 179, 626, 761, 1318, 1739
AsuII	TTCGAA	1 cut(s)	441
AvaI	CYCGRG	2 cut(s)	356, 418
AvaII	GGWCC	4 cut(s)	43, 93, 309, 850
BanI	GGYRCC	1 cut(s)	229
BbsI	GAAGAC	1 cut(s)	464
Bbv12I	GWGCWC	1 cut(s)	1929
BbvI	GCAGC	9 cut(s)	230, 291, 451, 461, 580, 592, 619, 677, 715
BccI	CCATC	3 cut(s)	344, 406, 861
BceAI	ACGGC	5 cut(s)	206, 231, 477, 1508, 1888
BciVI	GTATCC	1 cut(s)	694
BcnI	CCSGG	2 cut(s)	107, 429
BcoDI	GTCTC	3 cut(s)	22, 1062, 1138
BfaI	CTAG	3 cut(s)	609, 1724, 1778
BfoI	RGCGCY	1 cut(s)	654
BfuAI	ACCTGC	1 cut(s)	637
BfuI	GTATCC	1 cut(s)	694
Bme1390I	CCNGG	2 cut(s)	107, 429
Bme18I	GGWCC	4 cut(s)	43, 93, 309, 850
BmeT110I	CYCGRG	2 cut(s)	356, 418
BmgBI	CACGTC	1 cut(s)	268
BmgT120I	GGNCC	7 cut(s)	43, 93, 104, 309, 379, 850, 1492
BmiI	GGNNCC	5 cut(s)	95, 231, 310, 403, 1567
BmrFI	CCNGG	2 cut(s)	107, 429
BmrI	ACTGGG	1 cut(s)	91
BmsI	GCATC	2 cut(s)	973, 1819
BmuI	ACTGGG	1 cut(s)	91
BoxI	GACNNNNGTC	2 cut(s)	9, 1443
BpiI	GAAGAC	1 cut(s)	464
BpmI	CTGGAG	2 cut(s)	663, 1023
Bpu14I	TTCGAA	1 cut(s)	441
BpuEI	CTTGAG	1 cut(s)	693
BpuMI	CCSGG	2 cut(s)	107, 429
BsaAI	YACGTR	2 cut(s)	797, 1359
BsaBI	GATNNNNATC	1 cut(s)	442
BsaJI	CCNNGG	3 cut(s)	39, 291, 375
BsaWI	WCCGGW	2 cut(s)	232, 404
BsaXI	ACNNNNNCTCC	4 cut(s)	287, 317, 480, 510
Bsc4I	CCNNNNNNNGG	1 cut(s)	434
Bse118I	RCCGGY	4 cut(s)	226, 271, 316, 1897
Bse1I	ACTGG	4 cut(s)	97, 646, 661, 1652
Bse3DI	GCAATG	1 cut(s)	1044
Bse8I	GATNNNNATC	1 cut(s)	442
BseAI	TCCGGA	1 cut(s)	404
BseDI	CCNNGG	3 cut(s)	39, 291, 375
BseGI	GGATG	3 cut(s)	355, 964, 1771
BseJI	GATNNNNATC	1 cut(s)	442
BseLI	CCNNNNNNNGG	1 cut(s)	434
BseMI	GCAATG	1 cut(s)	1044
BseMII	CTCAG	4 cut(s)	932, 1135, 1487, 1587
BseNI	ACTGG	4 cut(s)	97, 646, 661, 1652
BseRI	GAGGAG	1 cut(s)	780
BseXI	GCAGC	9 cut(s)	230, 291, 451, 461, 580, 592, 619, 677, 715
BsgI	GTGCAG	1 cut(s)	864
Bsh1236I	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
BshFI	GGCC	7 cut(s)	105, 146, 380, 416, 1156, 1461, 1494
BshNI	GGYRCC	1 cut(s)	229
BsiHKAI	GWGCWC	1 cut(s)	1929
BsiHKCI	CYCGRG	2 cut(s)	356, 418
BsiWI	CGTACG	2 cut(s)	212, 1359
BslFI	GGGAC	2 cut(s)	322, 1352
BslI	CCNNNNNNNGG	1 cut(s)	434
BsmAI	GTCTC	3 cut(s)	22, 1062, 1138
BsmBI	CGTCTC	1 cut(s)	22
BsmFI	GGGAC	2 cut(s)	322, 1352
BsmI	GAATGC	1 cut(s)	1154
BsnI	GGCC	7 cut(s)	105, 146, 380, 416, 1156, 1461, 1494
BsoBI	CYCGRG	2 cut(s)	356, 418
Bsp119I	TTCGAA	1 cut(s)	441
Bsp1286I	GDGCHC	1 cut(s)	1929
Bsp13I	TCCGGA	1 cut(s)	404
Bsp1407I	TGTACA	1 cut(s)	1502
BspANI	GGCC	7 cut(s)	105, 146, 380, 416, 1156, 1461, 1494
BspCNI	CTCAG	4 cut(s)	931, 1134, 1488, 1586
BspEI	TCCGGA	1 cut(s)	404
BspFNI	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
BspHI	TCATGA	1 cut(s)	168
BspLI	GGNNCC	5 cut(s)	95, 231, 310, 403, 1567
BspMI	ACCTGC	1 cut(s)	637
BspPI	GGATC	5 cut(s)	166, 202, 950, 1372, 1724
BspT104I	TTCGAA	1 cut(s)	441
BspT107I	GGYRCC	1 cut(s)	229
BsrBI	CCGCTC	1 cut(s)	399
BsrDI	GCAATG	1 cut(s)	1044
BsrFI	RCCGGY	4 cut(s)	226, 271, 316, 1897
BsrGI	TGTACA	1 cut(s)	1502
BsrI	ACTGG	4 cut(s)	97, 646, 661, 1652
BssAI	RCCGGY	4 cut(s)	226, 271, 316, 1897
BssECI	CCNNGG	3 cut(s)	39, 291, 375
BssT1I	CCWWGG	2 cut(s)	39, 291
Bst4CI	ACNGT	5 cut(s)	28, 87, 202, 1271, 1594
Bst6I	CTCTTC	1 cut(s)	1190
BstAPI	GCANNNNNTGC	2 cut(s)	688, 1622
BstAUI	TGTACA	1 cut(s)	1502
BstBAI	YACGTR	2 cut(s)	797, 1359
BstBI	TTCGAA	1 cut(s)	441
BstC8I	GCNNGC	7 cut(s)	148, 425, 570, 843, 1154, 1320, 1895
BstDEI	CTNAG	5 cut(s)	669, 918, 1121, 1496, 1573
BstF5I	GGATG	3 cut(s)	355, 964, 1771
BstFNI	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
BstH2I	RGCGCY	1 cut(s)	654
BstHHI	GCGC	5 cut(s)	10, 252, 593, 653, 718
BstMAI	GTCTC	3 cut(s)	22, 1062, 1138
BstNSI	RCATGY	2 cut(s)	1322, 1620
BstPAI	GACNNNNGTC	2 cut(s)	9, 1443
BstSCI	CCNGG	2 cut(s)	105, 427
BstUI	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
BstV1I	GCAGC	9 cut(s)	230, 291, 451, 461, 580, 592, 619, 677, 715
BstV2I	GAAGAC	1 cut(s)	464
BsuI	GTATCC	1 cut(s)	694
BsuRI	GGCC	7 cut(s)	105, 146, 380, 416, 1156, 1461, 1494
BtrI	CACGTC	1 cut(s)	268
BtsCI	GGATG	3 cut(s)	355, 964, 1771
BtsI	GCAGTG	1 cut(s)	852
BtsIMutI	CAGTG	2 cut(s)	852, 1143
BveI	ACCTGC	1 cut(s)	637
Cac8I	GCNNGC	7 cut(s)	148, 425, 570, 843, 1154, 1320, 1895
CciI	TCATGA	1 cut(s)	168
CfoI	GCGC	5 cut(s)	10, 252, 593, 653, 718
Cfr10I	RCCGGY	4 cut(s)	226, 271, 316, 1897
Cfr13I	GGNCC	7 cut(s)	43, 93, 104, 309, 379, 850, 1492
CseI	GACGC	4 cut(s)	4, 14, 173, 1247
Csp6I	GTAC	6 cut(s)	24, 213, 230, 1360, 1503, 1694
CviQI	GTAC	6 cut(s)	24, 213, 230, 1360, 1503, 1694
DdeI	CTNAG	5 cut(s)	669, 918, 1121, 1496, 1573
DrdI	GACNNNNNNGTC	1 cut(s)	41
DseDI	GACNNNNNNGTC	1 cut(s)	41
EaeI	YGGCCR	1 cut(s)	144
Eam1104I	CTCTTC	1 cut(s)	1190
EarI	CTCTTC	1 cut(s)	1190
EciI	GGCGGA	1 cut(s)	35
Eco130I	CCWWGG	2 cut(s)	39, 291
Eco47I	GGWCC	4 cut(s)	43, 93, 309, 850
Eco57I	CTGAAG	3 cut(s)	489, 1010, 1214
Eco88I	CYCGRG	2 cut(s)	356, 418
EcoO109I	RGGNCCY	2 cut(s)	309, 1492
EcoT14I	CCWWGG	2 cut(s)	39, 291
EcoT22I	ATGCAT	3 cut(s)	966, 1542, 1642
ErhI	CCWWGG	2 cut(s)	39, 291
Esp3I	CGTCTC	1 cut(s)	22
FalI	AAGNNNNNCTT	4 cut(s)	1202, 1234, 1455, 1487
FaqI	GGGAC	2 cut(s)	322, 1352
FblI	GTMKAC	1 cut(s)	1437
FokI	GGATG	3 cut(s)	362, 951, 1778
FspBI	CTAG	3 cut(s)	609, 1724, 1778
GlaI	GCGC	5 cut(s)	9, 251, 592, 652, 717
GsuI	CTGGAG	2 cut(s)	663, 1023
HaeII	RGCGCY	1 cut(s)	654
HaeIII	GGCC	7 cut(s)	105, 146, 380, 416, 1156, 1461, 1494
HgaI	GACGC	4 cut(s)	4, 14, 173, 1247
HhaI	GCGC	5 cut(s)	10, 252, 593, 653, 718
Hin6I	GCGC	5 cut(s)	8, 250, 591, 651, 716
HinP1I	GCGC	5 cut(s)	8, 250, 591, 651, 716
HindIII	AAGCTT	2 cut(s)	1451, 1671
HphI	GGTGA	6 cut(s)	55, 179, 626, 761, 1318, 1739
Hpy166II	GTNNAC	2 cut(s)	1438, 1771
Hpy8I	GTNNAC	2 cut(s)	1438, 1771
Hpy99I	CGWCG	3 cut(s)	15, 184, 269
HpyAV	CCTTC	7 cut(s)	132, 342, 691, 729, 823, 1451, 1741
HpyCH4III	ACNGT	5 cut(s)	28, 87, 202, 1271, 1594
HpyCH4IV	ACGT	3 cut(s)	267, 796, 1358
HpyF3I	CTNAG	5 cut(s)	669, 918, 1121, 1496, 1573
HpySE526I	ACGT	3 cut(s)	267, 796, 1358
HspAI	GCGC	5 cut(s)	8, 250, 591, 651, 716
Kpn2I	TCCGGA	1 cut(s)	404
KpnI	GGTACC	1 cut(s)	233
LmnI	GCTCC	4 cut(s)	407, 1047, 1218, 1565
Lsp1109I	GCAGC	9 cut(s)	230, 291, 451, 461, 580, 592, 619, 677, 715
LweI	GCATC	2 cut(s)	973, 1819
MaeI	CTAG	3 cut(s)	609, 1724, 1778
MaeII	ACGT	3 cut(s)	267, 796, 1358
MaeIII	GTNAC	4 cut(s)	185, 637, 1546, 1861
MbiI	CCGCTC	1 cut(s)	399
MboII	GAAGA	6 cut(s)	52, 469, 787, 1207, 1343, 1770
MhlI	GDGCHC	1 cut(s)	1929
MluCI	AATT	7 cut(s)	526, 881, 1091, 1263, 1707, 1783, 1816
MlyI	GAGTC	7 cut(s)	305, 346, 348, 380, 1207, 1241, 1433
MmeI	TCCRAC	5 cut(s)	71, 219, 365, 964, 973
Mph1103I	ATGCAT	3 cut(s)	966, 1542, 1642
MroI	TCCGGA	1 cut(s)	404
MseI	TTAA	3 cut(s)	1109, 1220, 1820
MslI	CAYNNNNRTG	2 cut(s)	203, 1892
MspA1I	CMGCKG	1 cut(s)	605
MspR9I	CCNGG	2 cut(s)	107, 429
Mva1269I	GAATGC	1 cut(s)	1154
MvnI	CGCG	8 cut(s)	8, 10, 15, 184, 342, 591, 1552, 1911
NciI	CCSGG	2 cut(s)	107, 429
NlaIV	GGNNCC	5 cut(s)	95, 231, 310, 403, 1567
NmeAIII	GCCGAG	2 cut(s)	356, 845
NmuCI	GTSAC	2 cut(s)	185, 1861
NsiI	ATGCAT	3 cut(s)	966, 1542, 1642
NspI	RCATGY	2 cut(s)	1322, 1620
NspV	TTCGAA	1 cut(s)	441
OliI	CACNNNNGTG	2 cut(s)	203, 1892
PaeI	GCATGC	1 cut(s)	1322
PaeR7I	CTCGAG	2 cut(s)	356, 418
PagI	TCATGA	1 cut(s)	168
PcsI	WCGNNNNNNNCGW	3 cut(s)	12, 19, 363
PctI	GAATGC	1 cut(s)	1154
Pfl23II	CGTACG	2 cut(s)	212, 1359
PflFI	GACNNNGTC	2 cut(s)	97, 200
PleI	GAGTC	7 cut(s)	304, 345, 348, 379, 1206, 1240, 1433
PpsI	GAGTC	7 cut(s)	304, 345, 348, 379, 1206, 1240, 1433
Ppu21I	YACGTR	2 cut(s)	797, 1359
PpuMI	RGGWCCY	1 cut(s)	309
PshAI	GACNNNNGTC	2 cut(s)	9, 1443
PsiI	TTATAA	3 cut(s)	978, 1277, 1677
Psp5II	RGGWCCY	1 cut(s)	309
PspLI	CGTACG	2 cut(s)	212, 1359
PspN4I	GGNNCC	5 cut(s)	95, 231, 310, 403, 1567
PspPI	GGNCC	7 cut(s)	43, 93, 104, 309, 379, 850, 1492
PspPPI	RGGWCCY	1 cut(s)	309
PspXI	VCTCGAGB	2 cut(s)	356, 418
PsyI	GACNNNGTC	2 cut(s)	97, 200
RsaI	GTAC	6 cut(s)	25, 214, 231, 1361, 1504, 1695
RsaNI	GTAC	6 cut(s)	24, 213, 230, 1360, 1503, 1694
RseI	CAYNNNNRTG	2 cut(s)	203, 1892
SaqAI	TTAA	3 cut(s)	1109, 1220, 1820
Sau96I	GGNCC	7 cut(s)	43, 93, 104, 309, 379, 850, 1492
SchI	GAGTC	7 cut(s)	305, 346, 348, 380, 1207, 1241, 1433
ScrFI	CCNGG	2 cut(s)	107, 429
SduI	GDGCHC	1 cut(s)	1929
SfaNI	GCATC	2 cut(s)	973, 1819
Sfr274I	CTCGAG	2 cut(s)	356, 418
SfuI	TTCGAA	1 cut(s)	441
SgrAI	CRCCGGYG	1 cut(s)	316
SinI	GGWCC	4 cut(s)	43, 93, 309, 850
SlaI	CTCGAG	2 cut(s)	356, 418
SmiMI	CAYNNNNRTG	2 cut(s)	203, 1892
SmlI	CTYRAG	3 cut(s)	356, 418, 708
SmoI	CTYRAG	3 cut(s)	356, 418, 708
SphI	GCATGC	1 cut(s)	1322
Sse9I	AATT	7 cut(s)	526, 881, 1091, 1263, 1707, 1783, 1816
SspMI	CTAG	3 cut(s)	609, 1724, 1778
StyD4I	CCNGG	2 cut(s)	105, 427
StyI	CCWWGG	2 cut(s)	39, 291
TaaI	ACNGT	5 cut(s)	28, 87, 202, 1271, 1594
TaiI	ACGT	3 cut(s)	270, 799, 1361
TaqI	TCGA	8 cut(s)	33, 335, 357, 419, 441, 754, 1163, 1601
TasI	AATT	7 cut(s)	526, 881, 1091, 1263, 1707, 1783, 1816
TatI	WGTACW	1 cut(s)	1502
TauI	GCSGC	4 cut(s)	250, 402, 605, 1571
Tru1I	TTAA	3 cut(s)	1109, 1220, 1820
Tru9I	TTAA	3 cut(s)	1109, 1220, 1820
TscAI	CASTG	2 cut(s)	852, 1150
TseFI	GTSAC	2 cut(s)	185, 1861
TseI	GCWGC	9 cut(s)	218, 279, 449, 464, 593, 605, 632, 665, 728
Tsp45I	GTSAC	2 cut(s)	185, 1861
TspDTI	ATGAA	3 cut(s)	1296, 1419, 1557
TspGWI	ACGGA	6 cut(s)	200, 269, 1356, 1377, 1778, 1827
TspRI	CASTG	2 cut(s)	852, 1150
Tth111I	GACNNNGTC	2 cut(s)	97, 200
VpaK11BI	GGWCC	4 cut(s)	43, 93, 309, 850
XapI	RAATTY	3 cut(s)	526, 1091, 1816
XbaI	TCTAGA	1 cut(s)	1777
XceI	RCATGY	2 cut(s)	1322, 1620
XhoI	CTCGAG	2 cut(s)	356, 418
XmiI	GTMKAC	1 cut(s)	1437
XspI	CTAG	3 cut(s)	609, 1724, 1778
Zsp2I	ATGCAT	3 cut(s)	966, 1542, 1642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.