MD14G1230400.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011787
Physical Location & Seq
Reverse (-)
31116654 .. 31119297
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1230400.v1.1.491

Sequence Viewer

Length: 2130 bp
ATGGATCCACCGCCACTCCCGCCTCTCCCCACCACAATCACCACCACCGCAATTCCGGCAGCCACCAGCATGCAACTCAACTACCCCGACTCCGTCGACTCCTCCCCACGCTCCCGCAATGCTGACACCTGGGACGACCCCCTCCCTCAAGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGTGGTCACATCATTCCCCGCCCGCACGACAAGTCCCTCTGCTACGTCGGCGGAGAAACCCGCATGTTCGTTGCCGAGCGCCACTCCTCTCTCTCTGACCTCTGCTCCCGCCTCTCCCGAACCCTCCTCAACGGCCGCCCCTTCTCCCTCAAGTACCAGCTCCCCACCGAAGATCTCGACTCCCTCATCTCCGTCACCACCGACGAAGATCTCGACAACATGTTCGAAGAATACGACCGCACCTCCTCCGCCTCCCCCCTCAAGCCTTCCCGCCTCCGATTGTTTCTCTTCTTCGCGAAGCCGGAAACCGCCGCCTCGATGGGTGCTCTGCTCGACGACGCAAAGTCCGAGTCTTGGTTCGTCGACGCGCTGAACGGGTCGGGTTTTCCGAGGAACCTGTCGGATTCCGCCACCATGGATTACTTGCTGGCGGGGAGTGAGAATTCTTGCAATGATTTGGAGGCGCAAGGCGACTTCTTCGGAGGCAACAACAAACAGGACAACAACAATGGTTTGATGAAAAATGTTAATAATGTGGTCGGACATGATGGTCATTCGATCAATCAAGAGTCCACCTTCGCCGAGGACAGTACTTCCTCTTTCGGGTTGTCTTCCTCATCGACTTGCTTGTCGAATTTGCCTCCGATTCGGGTTCGAATGGACGAAACTGGTGATAGGTTGATGCAGGGTGGTCAGAGGACTGTTGGGATCGAGGAGCAGTTTTCTCAGATGACTACTAGTTATGCTGCTGCTGCTGTTGCTGCTCCGCCGCCAAAGGCGTCGAATTCGGGTGCTTTGCCGCCACCTGGTGCTCAGATGATGAATGTTTCTGGCGTTTCTGGAGAAAATATGAACAGGGTAGATGATGAGAGATCACCAGATCAGAGTATACCGGTCGGATTTCGTAAGCCTCCTCTGCCATTGCCGGTGCAGCCTCTGCAAGTTAATAAGGCTGGCGGCGGAGGAGGAGGTTATAGTTTGCCTTCACCGGATTCAGTTGCAAGTGATAGTAGCGTTGCATCCGCAAACTCTCTCTCCAAACCAATGTACTATCGAGAGCAGATTCAAGCTGCAAACAGGGACAACATCAGAGGTCCTGCTAGCCCGAATACACCCAGTGATGCTTCCGATCCGGGATCTCAAAACCAAGTTCAGCAAGTTCAGGATTCCGGGTATGCTATTCCTCAACAATCAGATCAGCAGCAGCAGCAGCAACAACAACAACAACTGCAGCAGCAGCAGCAGCAGCAGCAACAACTACAACAGCAGCAGCAGCAACAACAACAACAATATGTCCATGCCAGCACACATTACATGCACCATCCTGCCGGAGGCCAAGTGCCAATACAATCTTACTACACAATGTATGCACCGCCGTCACAGCAACAACCTCATCACCAAATCGATCACCAGCAGTATCCAGTCTATTTGATGCCTGTTGCGCAGTCACAACAATCGTACAACATCCCTTTGCAACCTAACATGTCTGACACAACCGTGGTTGCCTCAAGCGGCTTAACATCCCCCAATGCTGGCTCTGCCGTTTACAAGGACAGTAATATCCCACCTGTGTATCCCACAAACGCGGCTTCGCCTGAAATGTTTGCAAGTGTTTACAAAACGGCTGTGCCACAAACTCCAACATTAGTCCAACTTCCTTCTAACCAATACCAGCAGCAATACCTGGGGTTCTCTCAAATGCATCACCATCCTTCTCAGTCCGCTGCCATGCCTCCGCCTGCAACCACTAACTACGCTTACGAATACGCCAATCCTCAGCATGAACAAGCGTTCTACACACAGCATCAGGCCGCCCCATTGCCTCCTCAATACCAGTCTATGACTCCTGCAGCAGCGGCCGCAGCAGTGGCACTGTCAGAAGATTCGAAACAGCAGCTACCGGCATACTCAGCAGATCAGAACCACACAACCATAAGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

710

Amino Acids

77.09

Weight (kDa)

5.06

Isoelectric Point (pI)

68.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 71 - 159 4.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 735, 814
Acc16I TGCGCA 1 cut(s) 1629
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 3 cut(s) 96, 549, 1075
AccII CGCG 3 cut(s) 482, 554, 1772
AclWI GGATC 4 cut(s) 12, 902, 1310, 1330
AcoI YGGCCR 2 cut(s) 319, 2043
AcsI RAATTY 3 cut(s) 628, 820, 970
AcyI GRCGYC 2 cut(s) 159, 965
AdeI CACNNNGTG 2 cut(s) 995, 1304
AfaI GTAC 4 cut(s) 341, 778, 1235, 1646
AfiI CCNNNNNNNGG 5 cut(s) 540, 789, 992, 1517, 1718
AflIII ACRYGT 2 cut(s) 405, 1668
AgeI ACCGGT 1 cut(s) 1078
AgsI TTSAA 1 cut(s) 1253
AhdI GACNNNNNGTC 1 cut(s) 529
AhlI ACTAGT 1 cut(s) 923
AjnI CCWGG 3 cut(s) 128, 991, 1869
AleI CACNNNNGTG 1 cut(s) 1682
AluBI AGCT 5 cut(s) 166, 183, 346, 1256, 2083
AluI AGCT 5 cut(s) 166, 183, 346, 1256, 2083
Alw21I GWGCWC 2 cut(s) 514, 1000
AlwI GGATC 4 cut(s) 12, 902, 1310, 1330
AlwNI CAGNNNCTG 1 cut(s) 1123
AoxI GGCC 4 cut(s) 319, 1519, 1995, 2043
ApoI RAATTY 3 cut(s) 628, 820, 970
ArsI GACNNNNNNTTYG 4 cut(s) 392, 424, 521, 553
AsiGI ACCGGT 1 cut(s) 1078
AspLEI GCGC 5 cut(s) 161, 267, 556, 652, 1630
AspS9I GGNCC 1 cut(s) 1280
AsuC2I CCSGG 3 cut(s) 156, 1320, 1357
AsuHPI GGTGA 8 cut(s) 31, 373, 869, 1053, 1164, 1574, 1586, 1883
AsuII TTCGAA 3 cut(s) 411, 841, 2072
AsuNHI GCTAGC 1 cut(s) 1286
AvaII GGWCC 1 cut(s) 1280
BaeI ACNNNNGTAYC 2 cut(s) 1742, 1775
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 2 cut(s) 514, 1000
BbvCI CCTCAGC 1 cut(s) 1962
BccI CCATC 4 cut(s) 499, 728, 1515, 1902
BceAI ACGGC 4 cut(s) 334, 1546, 1712, 1824
BciT130I CCWGG 3 cut(s) 130, 993, 1871
BciVI GTATCC 2 cut(s) 1614, 1770
BcnI CCSGG 3 cut(s) 156, 1320, 1357
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 2 cut(s) 924, 1287
BfmI CTRYAG 2 cut(s) 1415, 2034
BfoI RGCGCY 2 cut(s) 162, 268
BfuI GTATCC 2 cut(s) 1614, 1770
BglII AGATCT 2 cut(s) 358, 394
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 6 cut(s) 130, 156, 993, 1320, 1357, 1871
Bme18I GGWCC 1 cut(s) 1280
BmeRI GACNNNNNGTC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 3 cut(s) 6, 160, 581
BmrFI CCNGG 6 cut(s) 130, 156, 993, 1320, 1357, 1871
BmrI ACTGGG 1 cut(s) 1296
BmsI GCATC 6 cut(s) 858, 1214, 1297, 1608, 1897, 1999
BmtI GCTAGC 1 cut(s) 1290
BmuI ACTGGG 1 cut(s) 1296
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 1047
Bpu10I CCTNAGC 1 cut(s) 1962
Bpu14I TTCGAA 3 cut(s) 411, 841, 2072
BpuEI CTTGAG 4 cut(s) 132, 320, 431, 1678
BpuMI CCSGG 3 cut(s) 156, 1320, 1357
Bsa29I ATCGAT 1 cut(s) 1590
BsaHI GRCGYC 2 cut(s) 159, 965
BsaJI CCNNGG 7 cut(s) 129, 154, 575, 600, 768, 1683, 1870
BsaWI WCCGGW 2 cut(s) 1078, 1174
Bsc4I CCNNNNNNNGG 5 cut(s) 540, 789, 992, 1517, 1718
Bse118I RCCGGY 3 cut(s) 1078, 1111, 2086
Bse1I ACTGG 4 cut(s) 859, 1302, 1607, 2020
Bse3DI GCAATG 4 cut(s) 124, 643, 1106, 2003
BseBI CCWGG 3 cut(s) 130, 993, 1871
BseCI ATCGAT 1 cut(s) 1590
BseDI CCNNGG 7 cut(s) 129, 154, 575, 600, 768, 1683, 1870
BseGI GGATG 5 cut(s) 1205, 1507, 1650, 1706, 1894
BseLI CCNNNNNNNGG 5 cut(s) 540, 789, 992, 1517, 1718
BseMI GCAATG 4 cut(s) 124, 643, 1106, 2003
BseMII CTCAG 5 cut(s) 926, 1013, 1916, 1976, 2109
BseNI ACTGG 4 cut(s) 859, 1302, 1607, 2020
BseRI GAGGAG 9 cut(s) 91, 262, 302, 421, 914, 1089, 1164, 1167, 2001
BseX3I CGGCCG 2 cut(s) 319, 2043
BsgI GTGCAG 2 cut(s) 199, 1136
Bsh1236I CGCG 3 cut(s) 482, 554, 1772
Bsh1285I CGRYCG 4 cut(s) 322, 424, 1083, 2046
BshFI GGCC 4 cut(s) 321, 1521, 1997, 2045
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 1078
BshVI ATCGAT 1 cut(s) 1590
BsiEI CGRYCG 4 cut(s) 322, 424, 1083, 2046
BsiHKAI GWGCWC 2 cut(s) 514, 1000
BslFI GGGAC 4 cut(s) 137, 146, 205, 1280
BslI CCNNNNNNNGG 5 cut(s) 540, 789, 992, 1517, 1718
BsmFI GGGAC 4 cut(s) 137, 146, 205, 1280
BsnI GGCC 4 cut(s) 321, 1521, 1997, 2045
Bsp119I TTCGAA 3 cut(s) 411, 841, 2072
Bsp1286I GDGCHC 2 cut(s) 514, 1000
Bsp19I CCATGG 1 cut(s) 600
Bsp68I TCGCGA 1 cut(s) 482
BspANI GGCC 4 cut(s) 321, 1521, 1997, 2045
BspCNI CTCAG 5 cut(s) 925, 1012, 1915, 1975, 2108
BspDI ATCGAT 1 cut(s) 1590
BspFNI CGCG 3 cut(s) 482, 554, 1772
BspLI GGNNCC 3 cut(s) 6, 160, 581
BspMAI CTGCAG 2 cut(s) 1419, 2038
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 4 cut(s) 12, 902, 1310, 1330
BspT104I TTCGAA 3 cut(s) 411, 841, 2072
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 4 cut(s) 124, 643, 1106, 2003
BsrFI RCCGGY 3 cut(s) 1078, 1111, 2086
BsrI ACTGG 4 cut(s) 859, 1302, 1607, 2020
BssAI RCCGGY 3 cut(s) 1078, 1111, 2086
BssECI CCNNGG 7 cut(s) 129, 154, 575, 600, 768, 1683, 1870
BssNAI GTATAC 1 cut(s) 1076
BssNI GRCGYC 2 cut(s) 159, 965
BssT1I CCWWGG 1 cut(s) 600
Bst1107I GTATAC 1 cut(s) 1076
Bst2UI CCWGG 3 cut(s) 130, 993, 1871
Bst4CI ACNGT 6 cut(s) 188, 776, 889, 1684, 1742, 2061
Bst6I CTCTTC 1 cut(s) 479
BstACI GRCGYC 2 cut(s) 159, 965
BstAPI GCANNNNNTGC 1 cut(s) 1123
BstBI TTCGAA 3 cut(s) 411, 841, 2072
BstC8I GCNNGC 8 cut(s) 71, 209, 615, 1141, 1288, 1489, 1720, 1926
BstDEI CTNAG 5 cut(s) 912, 999, 1902, 1962, 2095
BstDSI CCRYGG 2 cut(s) 600, 1683
BstENI CCTNNNNNAGG 1 cut(s) 1515
BstF5I GGATG 5 cut(s) 1205, 1507, 1650, 1706, 1894
BstFNI CGCG 3 cut(s) 482, 554, 1772
BstH2I RGCGCY 2 cut(s) 162, 268
BstHHI GCGC 5 cut(s) 161, 267, 556, 652, 1630
BstMCI CGRYCG 4 cut(s) 322, 424, 1083, 2046
BstNI CCWGG 3 cut(s) 130, 993, 1871
BstNSI RCATGY 5 cut(s) 73, 253, 409, 1504, 1672
BstSCI CCNGG 6 cut(s) 128, 154, 991, 1318, 1355, 1869
BstSFI CTRYAG 2 cut(s) 1415, 2034
BstUI CGCG 3 cut(s) 482, 554, 1772
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 4 cut(s) 4, 358, 394, 1322
BstYI RGATCY 4 cut(s) 4, 358, 394, 1322
BstZ17I GTATAC 1 cut(s) 1076
BstZI CGGCCG 2 cut(s) 319, 2043
Bsu15I ATCGAT 1 cut(s) 1590
BsuI GTATCC 2 cut(s) 1614, 1770
BsuRI GGCC 4 cut(s) 321, 1521, 1997, 2045
BsuTUI ATCGAT 1 cut(s) 1590
BtgI CCRYGG 2 cut(s) 600, 1683
BtsCI GGATG 5 cut(s) 1205, 1507, 1650, 1706, 1894
BtsI GCAGTG 1 cut(s) 2058
BtsIMutI CAGTG 3 cut(s) 1309, 2057, 2058
BtuMI TCGCGA 1 cut(s) 482
Cac8I GCNNGC 8 cut(s) 71, 209, 615, 1141, 1288, 1489, 1720, 1926
CaiI CAGNNNCTG 1 cut(s) 1123
CciNI GCGGCCGC 1 cut(s) 2043
CfoI GCGC 5 cut(s) 161, 267, 556, 652, 1630
Cfr10I RCCGGY 3 cut(s) 1078, 1111, 2086
Cfr13I GGNCC 1 cut(s) 1280
ClaI ATCGAT 1 cut(s) 1590
CseI GACGC 3 cut(s) 533, 560, 954
CsiI ACCWGGT 1 cut(s) 991
Csp6I GTAC 4 cut(s) 340, 777, 1234, 1645
CspAI ACCGGT 1 cut(s) 1078
CviQI GTAC 4 cut(s) 340, 777, 1234, 1645
DdeI CTNAG 5 cut(s) 912, 999, 1902, 1962, 2095
DinI GGCGCC 1 cut(s) 160
DraIII CACNNNGTG 2 cut(s) 995, 1304
DrdI GACNNNNNNGTC 2 cut(s) 735, 814
DriI GACNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 2 cut(s) 735, 814
EaeI YGGCCR 2 cut(s) 319, 2043
EagI CGGCCG 2 cut(s) 319, 2043
Eam1104I CTCTTC 1 cut(s) 479
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 479
EciI GGCGGA 7 cut(s) 158, 252, 424, 583, 942, 1161, 1911
EclXI CGGCCG 2 cut(s) 319, 2043
Eco130I CCWWGG 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 1280
Eco52I CGGCCG 2 cut(s) 319, 2043
EcoNI CCTNNNNNAGG 1 cut(s) 1515
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRI GAATTC 2 cut(s) 628, 970
EcoRII CCWGG 3 cut(s) 128, 991, 1869
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 1890
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 600
FaqI GGGAC 4 cut(s) 137, 146, 205, 1280
FauI CCCGC 8 cut(s) 27, 122, 212, 216, 254, 302, 464, 610
FblI GTMKAC 3 cut(s) 96, 549, 1075
FokI GGATG 5 cut(s) 1192, 1494, 1637, 1693, 1881
FspBI CTAG 2 cut(s) 924, 1287
FspI TGCGCA 1 cut(s) 1629
GlaI GCGC 5 cut(s) 160, 266, 555, 651, 1629
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 2 cut(s) 162, 268
HaeIII GGCC 4 cut(s) 321, 1521, 1997, 2045
HgaI GACGC 3 cut(s) 533, 560, 954
HhaI GCGC 5 cut(s) 161, 267, 556, 652, 1630
Hin1I GRCGYC 2 cut(s) 159, 965
Hin6I GCGC 5 cut(s) 159, 265, 554, 650, 1628
HinP1I GCGC 5 cut(s) 159, 265, 554, 650, 1628
HincII GTYRAC 2 cut(s) 97, 550
HindII GTYRAC 2 cut(s) 97, 550
HphI GGTGA 8 cut(s) 31, 373, 869, 1053, 1164, 1574, 1586, 1883
Hpy166II GTNNAC 6 cut(s) 97, 550, 759, 1076, 1732, 1801
Hpy188III TCNNGA 8 cut(s) 303, 362, 398, 481, 752, 1026, 1241, 1349
Hpy8I GTNNAC 6 cut(s) 97, 550, 759, 1076, 1732, 1801
Hpy99I CGWCG 8 cut(s) 98, 236, 392, 524, 527, 551, 554, 970
HpyAV CCTTC 6 cut(s) 337, 462, 772, 1179, 1854, 1908
HpyCH4III ACNGT 6 cut(s) 188, 776, 889, 1684, 1742, 2061
HpyCH4IV ACGT 1 cut(s) 231
HpyF3I CTNAG 5 cut(s) 912, 999, 1902, 1962, 2095
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 2 cut(s) 159, 965
HspAI GCGC 5 cut(s) 159, 265, 554, 650, 1628
KasI GGCGCC 1 cut(s) 158
LmnI GCTCC 6 cut(s) 116, 171, 296, 351, 901, 955
LweI GCATC 6 cut(s) 858, 1214, 1297, 1608, 1897, 1999
MabI ACCWGGT 1 cut(s) 991
MaeI CTAG 2 cut(s) 924, 1287
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 4 cut(s) 191, 379, 1563, 1632
MboII GAAGA 8 cut(s) 368, 404, 425, 466, 469, 655, 789, 2078
MflI RGATCY 4 cut(s) 4, 358, 394, 1322
MhlI GDGCHC 2 cut(s) 514, 1000
MluCI AATT 4 cut(s) 51, 628, 820, 970
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 6 cut(s) 83, 92, 359, 545, 764, 2023
MmeI TCCRAC 5 cut(s) 567, 706, 1063, 1850, 1861
Mph1103I ATGCAT 1 cut(s) 1890
MseI TTAA 3 cut(s) 714, 1131, 1703
MslI CAYNNNNRTG 2 cut(s) 68, 1682
MspA1I CMGCKG 2 cut(s) 1910, 2042
MspR9I CCNGG 6 cut(s) 130, 156, 993, 1320, 1357, 1871
MvaI CCWGG 3 cut(s) 130, 993, 1871
MvnI CGCG 3 cut(s) 482, 554, 1772
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 3 cut(s) 156, 1320, 1357
NcoI CCATGG 1 cut(s) 600
NheI GCTAGC 1 cut(s) 1286
NlaIV GGNNCC 3 cut(s) 6, 160, 581
NmeAIII GCCGAG 2 cut(s) 286, 793
NmuCI GTSAC 4 cut(s) 191, 379, 1563, 1632
NotI GCGGCCGC 1 cut(s) 2043
NruI TCGCGA 1 cut(s) 482
NsbI TGCGCA 1 cut(s) 1629
NsiI ATGCAT 1 cut(s) 1890
NspI RCATGY 5 cut(s) 73, 253, 409, 1504, 1672
NspV TTCGAA 3 cut(s) 411, 841, 2072
OliI CACNNNNGTG 1 cut(s) 1682
PaeI GCATGC 1 cut(s) 73
PciI ACATGT 2 cut(s) 405, 1668
PcsI WCGNNNNNNNCGW 4 cut(s) 396, 417, 531, 572
PfeI GAWTC 6 cut(s) 590, 832, 1178, 1249, 1352, 2069
PflFI GACNNNGTC 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 1318
PinAI ACCGGT 1 cut(s) 1078
PleI GAGTC 6 cut(s) 83, 92, 359, 544, 763, 2023
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 6 cut(s) 83, 92, 359, 544, 763, 2023
PpuMI RGGWCCY 1 cut(s) 1280
PscI ACATGT 2 cut(s) 405, 1668
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 3 cut(s) 128, 991, 1869
PspGI CCWGG 3 cut(s) 128, 991, 1869
PspN4I GGNNCC 3 cut(s) 6, 160, 581
PspPI GGNCC 1 cut(s) 1280
PspPPI RGGWCCY 1 cut(s) 1280
PstI CTGCAG 2 cut(s) 1419, 2038
PstNI CAGNNNCTG 1 cut(s) 1123
PsuI RGATCY 4 cut(s) 4, 358, 394, 1322
PsyI GACNNNGTC 1 cut(s) 92
RruI TCGCGA 1 cut(s) 482
RsaI GTAC 4 cut(s) 341, 778, 1235, 1646
RsaNI GTAC 4 cut(s) 340, 777, 1234, 1645
RseI CAYNNNNRTG 2 cut(s) 68, 1682
SalI GTCGAC 2 cut(s) 95, 548
SaqAI TTAA 3 cut(s) 714, 1131, 1703
Sau96I GGNCC 1 cut(s) 1280
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 6 cut(s) 83, 92, 359, 545, 764, 2023
ScrFI CCNGG 6 cut(s) 130, 156, 993, 1320, 1357, 1871
SduI GDGCHC 2 cut(s) 514, 1000
SexAI ACCWGGT 1 cut(s) 991
SfaNI GCATC 6 cut(s) 858, 1214, 1297, 1608, 1897, 1999
SfcI CTRYAG 2 cut(s) 1415, 2034
SfoI GGCGCC 1 cut(s) 160
SfuI TTCGAA 3 cut(s) 411, 841, 2072
SgrDI CGTCGACG 1 cut(s) 548
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 2 cut(s) 68, 1682
SmlI CTYRAG 4 cut(s) 147, 335, 446, 1693
SmoI CTYRAG 4 cut(s) 147, 335, 446, 1693
SpeI ACTAGT 1 cut(s) 923
SphI GCATGC 1 cut(s) 73
Sse9I AATT 4 cut(s) 51, 628, 820, 970
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 924, 1287
StyD4I CCNGG 6 cut(s) 128, 154, 991, 1318, 1355, 1869
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 6 cut(s) 188, 776, 889, 1684, 1742, 2061
TaiI ACGT 1 cut(s) 234
TasI AATT 4 cut(s) 51, 628, 820, 970
TatI WGTACW 2 cut(s) 776, 1233
TfiI GAWTC 6 cut(s) 590, 832, 1178, 1249, 1352, 2069
Tru1I TTAA 3 cut(s) 714, 1131, 1703
Tru9I TTAA 3 cut(s) 714, 1131, 1703
TscAI CASTG 3 cut(s) 1309, 2058, 2064
TseFI GTSAC 4 cut(s) 191, 379, 1563, 1632
Tsp45I GTSAC 4 cut(s) 191, 379, 1563, 1632
TspDTI ATGAA 4 cut(s) 719, 1022, 1052, 1983
TspGWI ACGGA 2 cut(s) 82, 367
TspRI CASTG 3 cut(s) 1309, 2058, 2064
Tth111I GACNNNGTC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 1280
XagI CCTNNNNNAGG 1 cut(s) 1515
XapI RAATTY 3 cut(s) 628, 820, 970
XceI RCATGY 5 cut(s) 73, 253, 409, 1504, 1672
XmiI GTMKAC 3 cut(s) 96, 549, 1075
XspI CTAG 2 cut(s) 924, 1287
ZrmI AGTACT 1 cut(s) 778
Zsp2I ATGCAT 1 cut(s) 1890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.