RchiOBHm_Chr7g0179361

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011787
Physical Location & Seq
Forward (+)
1672079 .. 1675468
3390 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15988

Sequence Viewer

Length: 2088 bp
ATGGATCCACCGCCTCTCCCACCACTTCCGGCCGCCACGCAACTCGGCTACCCCGATTCACTCGACTCCTCCCCCCGATCCCGCAATGCCGACTCTTCTTGGGAAGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTCGTCGCCGACCGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAACGGCCGTCCTTTCTCTCTCAAGTACCAGCTCCCGACGGAGGACCTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTGGACAACATGGTGGAGGAGTACGACCGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTCCGTCTCTTTCTTTTCTTCGGCAAACCGGAGACGGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCCGACTCGGCGGCCACCATGGACTGCTTGTTGACCGGCGGGAGTGATTCTTGCAACGACTTGGAGGCGGCGGCGGCGAGTCAAGGCGACTTGTTTGGGGATCATGACAGTGGAAAACCTAGTGTGGCGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCCTTTGTGGAGAGCAATACGTCGTCCTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCAAATTTGCCGCCAATTAGGGTTAGAATGGACGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCGGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTGCTGCTGCACCAACAATGGTGGCTGCTGGTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCTGGTCAGAATGTGCCTGTGGGTTTTCGAAAGCCGCCTTTGCCAATGCCATTGCAGCCGCAGCATTTTAAGCAGGGAGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCGGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCGCTGTCGAAACCAATGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTCCTGTTAGCCCGAATACAACAAGTGATGCTTTGGATCAGGGTTCCCGAAACCAAGTTCAACAAGTTCAGGATTCAGGGTACAGTGTGCCTCCGCAATCAGATCAACAACAGCAAATTCAGCTTCAGCAACAACAGCAGCAACAGCAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAGCAGCAGCAACAATTTGTCCATGCAAGCGCGCAATATATGAATATTCAGCACCCTGGTACCGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAGTATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGATGGCTCTGCAGTCTACAATAGCTGATCCAACTGGTGTTGCCTCGACCCGCATTACTTCCCAAAACCCTGCTGCTTACAAGGACAGTATCCCACCCATTTATCCCTCAAAGACAGCTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTGTGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCAATATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCGTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCCATGACTCCAGCAGCCGCCGCCGCAGCAGTAGCAATGTCAGATGAATCAAAGCAGAAAAACCAGATGCAGATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

695

Amino Acids

75.04

Weight (kDa)

5.06

Isoelectric Point (pI)

70.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 62 - 152 1.3e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1765
AasI GACNNNNNNGTC 1 cut(s) 478
Acc36I ACCTGC 1 cut(s) 1765
Acc65I GGTACC 1 cut(s) 1493
AccB1I GGYRCC 2 cut(s) 131, 1493
AccI GTMKAC 3 cut(s) 528, 1649, 1962
AccII CGCG 4 cut(s) 436, 533, 559, 1466
AclWI GGATC 7 cut(s) 12, 72, 375, 675, 992, 1260, 1655
AcoI YGGCCR 5 cut(s) 30, 163, 292, 579, 825
AcsI RAATTY 2 cut(s) 787, 1332
AcuI CTGAAG 1 cut(s) 1325
AcyI GRCGYC 3 cut(s) 132, 479, 504
AfaI GTAC 9 cut(s) 280, 314, 392, 937, 1175, 1298, 1495, 1520, 1931
AfiI CCNNNNNNNGG 3 cut(s) 328, 519, 1496
AgeI ACCGGT 1 cut(s) 1496
AgsI TTSAA 4 cut(s) 424, 701, 1193, 1277
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 1 cut(s) 1489
Alw21I GWGCWC 1 cut(s) 1219
Alw26I GTCTC 3 cut(s) 209, 449, 464
AlwI GGATC 7 cut(s) 12, 72, 375, 675, 992, 1260, 1655
Ama87I CYCGRG 1 cut(s) 544
AoxI GGCC 7 cut(s) 30, 163, 292, 489, 579, 726, 825
ApoI RAATTY 2 cut(s) 787, 1332
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
AsiGI ACCGGT 1 cut(s) 1496
Asp718I GGTACC 1 cut(s) 1493
AspLEI GCGC 4 cut(s) 134, 535, 1466, 1468
AspS9I GGNCC 3 cut(s) 124, 331, 489
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 6 cut(s) 346, 980, 1104, 1506, 1556, 1746
AsuII TTCGAA 1 cut(s) 1033
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 2 cut(s) 124, 331
AxyI CCTNAGG 1 cut(s) 120
BaeI ACNNNNGTAYC 2 cut(s) 1477, 1510
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 131, 1493
BanII GRGCYC 1 cut(s) 1219
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 1219
BccI CCATC 3 cut(s) 1202, 1549, 1630
BceAI ACGGC 7 cut(s) 175, 279, 307, 403, 412, 489, 556
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 1491
BciVI GTATCC 2 cut(s) 1599, 1733
BclI TGATCA 1 cut(s) 1573
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 3 cut(s) 209, 449, 464
BfaI CTAG 2 cut(s) 687, 1203
BfmI CTRYAG 2 cut(s) 1373, 1643
BfoI RGCGCY 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 1765
BfuI GTATCC 2 cut(s) 1599, 1733
BmcAI AGTACT 1 cut(s) 937
Bme1390I CCNGG 2 cut(s) 129, 1491
Bme18I GGWCC 2 cut(s) 124, 331
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 124, 331, 489
BmiI GGNNCC 7 cut(s) 6, 126, 133, 440, 490, 1261, 1495
BmrFI CCNGG 2 cut(s) 129, 1491
BmsI GCATC 7 cut(s) 543, 1234, 1426, 1593, 1882, 1984, 2064
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 3 cut(s) 945, 1406, 2001
Bpu14I TTCGAA 1 cut(s) 1033
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 129
BsaBI GATNNNNATC 1 cut(s) 2078
BsaHI GRCGYC 3 cut(s) 132, 479, 504
BsaJI CCNNGG 4 cut(s) 127, 483, 585, 1489
BsaWI WCCGGW 3 cut(s) 466, 1114, 1496
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 519, 1496
Bse118I RCCGGY 3 cut(s) 602, 850, 1496
Bse1I ACTGG 3 cut(s) 1508, 1592, 1672
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 6 cut(s) 91, 1055, 1140, 1890, 1988, 2049
Bse8I GATNNNNATC 1 cut(s) 2078
BseBI CCWGG 1 cut(s) 1491
BseDI CCNNGG 4 cut(s) 127, 483, 585, 1489
BseGI GGATG 3 cut(s) 168, 1003, 1876
BseJI GATNNNNATC 1 cut(s) 2078
BseLI CCNNNNNNNGG 3 cut(s) 328, 519, 1496
BseMI GCAATG 6 cut(s) 91, 1055, 1140, 1890, 1988, 2049
BseMII CTCAG 4 cut(s) 134, 890, 972, 1898
BseNI ACTGG 3 cut(s) 1508, 1592, 1672
BsePI GCGCGC 1 cut(s) 1464
BseRI GAGGAG 9 cut(s) 58, 235, 275, 394, 397, 401, 878, 978, 1800
BseX3I CGGCCG 2 cut(s) 30, 292
BseYI CCCAGC 1 cut(s) 1971
BsgI GTGCAG 3 cut(s) 172, 857, 879
Bsh1236I CGCG 4 cut(s) 436, 533, 559, 1466
Bsh1285I CGRYCG 4 cut(s) 33, 238, 295, 397
BshFI GGCC 7 cut(s) 32, 165, 294, 491, 581, 728, 827
BshNI GGYRCC 2 cut(s) 131, 1493
BshTI ACCGGT 1 cut(s) 1496
BsiEI CGRYCG 4 cut(s) 33, 238, 295, 397
BsiHKAI GWGCWC 1 cut(s) 1219
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 8 cut(s) 29, 129, 467, 603, 828, 851, 1115, 1497
BsiWI CGTACG 1 cut(s) 1929
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 3 cut(s) 328, 519, 1496
BsmAI GTCTC 3 cut(s) 209, 449, 464
BsmBI CGTCTC 2 cut(s) 449, 464
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 7 cut(s) 32, 165, 294, 491, 581, 728, 827
BsoBI CYCGRG 1 cut(s) 544
Bsp119I TTCGAA 1 cut(s) 1033
Bsp1286I GDGCHC 1 cut(s) 1219
Bsp19I CCATGG 2 cut(s) 483, 585
BspANI GGCC 7 cut(s) 32, 165, 294, 491, 581, 728, 827
BspCNI CTCAG 4 cut(s) 133, 889, 971, 1897
BspFNI CGCG 4 cut(s) 436, 533, 559, 1466
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 7 cut(s) 6, 126, 133, 440, 490, 1261, 1495
BspMAI CTGCAG 1 cut(s) 1647
BspMI ACCTGC 1 cut(s) 1765
BspPI GGATC 7 cut(s) 12, 72, 375, 675, 992, 1260, 1655
BspT104I TTCGAA 1 cut(s) 1033
BspT107I GGYRCC 2 cut(s) 131, 1493
BsrDI GCAATG 6 cut(s) 91, 1055, 1140, 1890, 1988, 2049
BsrFI RCCGGY 3 cut(s) 602, 850, 1496
BsrI ACTGG 3 cut(s) 1508, 1592, 1672
BssAI RCCGGY 3 cut(s) 602, 850, 1496
BssECI CCNNGG 4 cut(s) 127, 483, 585, 1489
BssHII GCGCGC 1 cut(s) 1464
BssNAI GTATAC 1 cut(s) 1963
BssNI GRCGYC 3 cut(s) 132, 479, 504
BssT1I CCWWGG 2 cut(s) 483, 585
Bst1107I GTATAC 1 cut(s) 1963
Bst2UI CCWGG 1 cut(s) 1491
Bst4CI ACNGT 5 cut(s) 677, 1301, 1518, 1617, 1721
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 3 cut(s) 132, 479, 504
BstAPI GCANNNNNTGC 1 cut(s) 1369
BstBI TTCGAA 1 cut(s) 1033
BstC8I GCNNGC 5 cut(s) 182, 852, 1106, 1462, 1466
BstDEI CTNAG 4 cut(s) 120, 876, 958, 1884
BstDSI CCRYGG 2 cut(s) 483, 585
BstF5I GGATG 3 cut(s) 168, 1003, 1876
BstFNI CGCG 4 cut(s) 436, 533, 559, 1466
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 4 cut(s) 134, 535, 1466, 1468
BstMAI GTCTC 3 cut(s) 209, 449, 464
BstMCI CGRYCG 4 cut(s) 33, 238, 295, 397
BstNI CCWGG 1 cut(s) 1491
BstSCI CCNGG 2 cut(s) 127, 1489
BstSFI CTRYAG 2 cut(s) 1373, 1643
BstUI CGCG 4 cut(s) 436, 533, 559, 1466
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 2 cut(s) 4, 367
BstYI RGATCY 2 cut(s) 4, 367
BstZ17I GTATAC 1 cut(s) 1963
BstZI CGGCCG 2 cut(s) 30, 292
Bsu36I CCTNAGG 1 cut(s) 120
BsuI GTATCC 2 cut(s) 1599, 1733
BsuRI GGCC 7 cut(s) 32, 165, 294, 491, 581, 728, 827
BtgI CCRYGG 2 cut(s) 483, 585
BtgZI GCGATG 1 cut(s) 1527
BtsCI GGATG 3 cut(s) 168, 1003, 1876
BtsIMutI CAGTG 3 cut(s) 682, 1306, 1515
BveI ACCTGC 1 cut(s) 1765
Cac8I GCNNGC 5 cut(s) 182, 852, 1106, 1462, 1466
CciI TCATGA 1 cut(s) 670
CfoI GCGC 4 cut(s) 134, 535, 1466, 1468
Cfr10I RCCGGY 3 cut(s) 602, 850, 1496
Cfr13I GGNCC 3 cut(s) 124, 331, 489
CseI GACGC 4 cut(s) 468, 512, 539, 1134
Csp6I GTAC 9 cut(s) 279, 313, 391, 936, 1174, 1297, 1494, 1519, 1930
CspAI ACCGGT 1 cut(s) 1496
CspCI CAANNNNNGTGG 2 cut(s) 888, 923
CviAII CATG 8 cut(s) 148, 379, 484, 586, 671, 1457, 1952, 2011
CviQI GTAC 9 cut(s) 279, 313, 391, 936, 1174, 1297, 1494, 1519, 1930
DdeI CTNAG 4 cut(s) 120, 876, 958, 1884
DinI GGCGCC 1 cut(s) 133
DrdI GACNNNNNNGTC 1 cut(s) 478
DriI GACNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 5 cut(s) 30, 163, 292, 579, 825
EagI CGGCCG 2 cut(s) 30, 292
Eam1104I CTCTTC 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 100
EciI GGCGGA 2 cut(s) 131, 400
Ecl136II GAGCTC 1 cut(s) 1217
EclXI CGGCCG 2 cut(s) 30, 292
Eco130I CCWWGG 2 cut(s) 483, 585
Eco24I GRGCYC 1 cut(s) 1219
Eco47I GGWCC 2 cut(s) 124, 331
Eco52I CGGCCG 2 cut(s) 30, 292
Eco53kI GAGCTC 1 cut(s) 1217
Eco57I CTGAAG 1 cut(s) 1325
Eco81I CCTNAGG 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 1217
EcoO109I RGGNCCY 3 cut(s) 124, 331, 489
EcoRII CCWGG 1 cut(s) 1489
EcoT14I CCWWGG 2 cut(s) 483, 585
EcoT22I ATGCAT 1 cut(s) 1875
EcoT38I GRGCYC 1 cut(s) 1219
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 483, 585
Esp3I CGTCTC 2 cut(s) 449, 464
FaeI CATG 8 cut(s) 151, 382, 487, 589, 674, 1460, 1955, 2014
FalI AAGNNNNNCTT 2 cut(s) 1231, 1263
FaqI GGGAC 1 cut(s) 110
FatI CATG 8 cut(s) 147, 378, 483, 585, 670, 1456, 1951, 2010
FauI CCCGC 7 cut(s) 89, 185, 189, 275, 599, 847, 1691
FbaI TGATCA 1 cut(s) 1573
FblI GTMKAC 3 cut(s) 528, 1649, 1962
FokI GGATG 3 cut(s) 155, 1010, 1863
FriOI GRGCYC 1 cut(s) 1219
FspBI CTAG 2 cut(s) 687, 1203
GlaI GCGC 4 cut(s) 133, 534, 1465, 1467
GsaI CCCAGC 1 cut(s) 1975
GsuI CTGGAG 3 cut(s) 945, 1406, 2001
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 7 cut(s) 32, 165, 294, 491, 581, 728, 827
HapII CCGG 8 cut(s) 29, 129, 467, 603, 828, 851, 1115, 1497
HgaI GACGC 4 cut(s) 468, 512, 539, 1134
HhaI GCGC 4 cut(s) 134, 535, 1466, 1468
Hin1I GRCGYC 3 cut(s) 132, 479, 504
Hin1II CATG 8 cut(s) 151, 382, 487, 589, 674, 1460, 1955, 2014
Hin6I GCGC 4 cut(s) 132, 533, 1464, 1466
HinP1I GCGC 4 cut(s) 132, 533, 1464, 1466
HincII GTYRAC 2 cut(s) 529, 600
HindII GTYRAC 2 cut(s) 529, 600
HindIII AAGCTT 1 cut(s) 883
HpaII CCGG 8 cut(s) 29, 129, 467, 603, 828, 851, 1115, 1497
HphI GGTGA 6 cut(s) 346, 980, 1104, 1506, 1556, 1746
Hpy166II GTNNAC 6 cut(s) 529, 600, 955, 1650, 1786, 1963
Hpy188III TCNNGA 9 cut(s) 322, 371, 671, 701, 924, 960, 1181, 1263, 1286
Hpy8I GTNNAC 6 cut(s) 529, 600, 955, 1650, 1786, 1963
HpyAV CCTTC 6 cut(s) 1119, 1839, 1890, 1911, 1956, 2004
HpyCH4III ACNGT 5 cut(s) 677, 1301, 1518, 1617, 1721
HpyCH4IV ACGT 2 cut(s) 204, 746
HpyF3I CTNAG 4 cut(s) 120, 876, 958, 1884
HpySE526I ACGT 2 cut(s) 204, 746
Hsp92I GRCGYC 3 cut(s) 132, 479, 504
Hsp92II CATG 8 cut(s) 151, 382, 487, 589, 674, 1460, 1955, 2014
HspAI GCGC 4 cut(s) 132, 533, 1464, 1466
KasI GGCGCC 1 cut(s) 131
KpnI GGTACC 1 cut(s) 1497
KroI GCCGGC 1 cut(s) 850
KroNI GCCGGC 1 cut(s) 852
Ksp22I TGATCA 1 cut(s) 1573
LmnI GCTCC 9 cut(s) 144, 269, 324, 444, 865, 926, 1222, 1410, 1540
LweI GCATC 7 cut(s) 543, 1234, 1426, 1593, 1882, 1984, 2064
MaeI CTAG 2 cut(s) 687, 1203
MaeII ACGT 2 cut(s) 204, 746
MaeIII GTNAC 4 cut(s) 352, 1512, 1798, 1859
MboII GAAGA 4 cut(s) 87, 448, 756, 759
MflI RGATCY 2 cut(s) 4, 367
MhlI GDGCHC 1 cut(s) 1219
MluCI AATT 8 cut(s) 787, 798, 1332, 1448, 1569, 1814, 1850, 2083
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 9 cut(s) 59, 86, 211, 332, 524, 566, 655, 1768, 2008
MmeI TCCRAC 5 cut(s) 279, 594, 1432, 1688, 1857
Mph1103I ATGCAT 1 cut(s) 1875
MroNI GCCGGC 1 cut(s) 850
MseI TTAA 1 cut(s) 1074
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 8 cut(s) 29, 129, 467, 603, 828, 851, 1115, 1497
MspR9I CCNGG 2 cut(s) 129, 1491
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 1 cut(s) 1491
MvnI CGCG 4 cut(s) 436, 533, 559, 1466
NaeI GCCGGC 1 cut(s) 852
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 2 cut(s) 483, 585
NgoMIV GCCGGC 1 cut(s) 850
NlaIII CATG 8 cut(s) 151, 382, 487, 589, 674, 1460, 1955, 2014
NlaIV GGNNCC 7 cut(s) 6, 126, 133, 440, 490, 1261, 1495
NmeAIII GCCGAG 3 cut(s) 24, 133, 554
NmuCI GTSAC 3 cut(s) 352, 1512, 1798
NsiI ATGCAT 1 cut(s) 1875
NspV TTCGAA 1 cut(s) 1033
PagI TCATGA 1 cut(s) 670
PaqCI CACCTGC 1 cut(s) 1765
PauI GCGCGC 1 cut(s) 1464
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 210, 510, 767
PctI GAATGC 1 cut(s) 709
PdiI GCCGGC 1 cut(s) 852
PfeI GAWTC 6 cut(s) 56, 614, 722, 1118, 1289, 2054
Pfl23II CGTACG 1 cut(s) 1929
PinAI ACCGGT 1 cut(s) 1496
PleI GAGTC 9 cut(s) 59, 86, 211, 332, 523, 566, 654, 1768, 2008
PluTI GGCGCC 1 cut(s) 135
PpsI GAGTC 9 cut(s) 59, 86, 211, 332, 523, 566, 654, 1768, 2008
PpuMI RGGWCCY 2 cut(s) 124, 331
Psp124BI GAGCTC 1 cut(s) 1219
Psp5II RGGWCCY 2 cut(s) 124, 331
Psp6I CCWGG 1 cut(s) 1489
PspFI CCCAGC 1 cut(s) 1971
PspGI CCWGG 1 cut(s) 1489
PspLI CGTACG 1 cut(s) 1929
PspN4I GGNNCC 7 cut(s) 6, 126, 133, 440, 490, 1261, 1495
PspPI GGNCC 3 cut(s) 124, 331, 489
PspPPI RGGWCCY 2 cut(s) 124, 331
PstI CTGCAG 1 cut(s) 1647
PsuI RGATCY 2 cut(s) 4, 367
PteI GCGCGC 1 cut(s) 1464
RsaI GTAC 9 cut(s) 280, 314, 392, 937, 1175, 1298, 1495, 1520, 1931
RsaNI GTAC 9 cut(s) 279, 313, 391, 936, 1174, 1297, 1494, 1519, 1930
RseI CAYNNNNRTG 1 cut(s) 675
SacI GAGCTC 1 cut(s) 1219
SalI GTCGAC 1 cut(s) 527
SaqAI TTAA 1 cut(s) 1074
Sau96I GGNCC 3 cut(s) 124, 331, 489
ScaI AGTACT 1 cut(s) 937
SchI GAGTC 9 cut(s) 59, 86, 211, 332, 524, 566, 655, 1768, 2008
ScrFI CCNGG 2 cut(s) 129, 1491
SduI GDGCHC 1 cut(s) 1219
SfaNI GCATC 7 cut(s) 543, 1234, 1426, 1593, 1882, 1984, 2064
SfcI CTRYAG 2 cut(s) 1373, 1643
SfoI GGCGCC 1 cut(s) 133
SfuI TTCGAA 1 cut(s) 1033
SgrDI CGTCGACG 1 cut(s) 527
SinI GGWCC 2 cut(s) 124, 331
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 8 cut(s) 787, 798, 1332, 1448, 1569, 1814, 1850, 2083
SspDI GGCGCC 1 cut(s) 131
SspI AATATT 1 cut(s) 1480
SspMI CTAG 2 cut(s) 687, 1203
SstI GAGCTC 1 cut(s) 1219
StyD4I CCNGG 2 cut(s) 127, 1489
StyI CCWWGG 2 cut(s) 483, 585
TaaI ACNGT 5 cut(s) 677, 1301, 1518, 1617, 1721
TaiI ACGT 2 cut(s) 207, 749
TaqI TCGA 8 cut(s) 63, 336, 498, 528, 713, 1033, 1163, 1679
TasI AATT 8 cut(s) 787, 798, 1332, 1448, 1569, 1814, 1850, 2083
TatI WGTACW 2 cut(s) 935, 1173
TfiI GAWTC 6 cut(s) 56, 614, 722, 1118, 1289, 2054
Tru1I TTAA 1 cut(s) 1074
Tru9I TTAA 1 cut(s) 1074
TscAI CASTG 3 cut(s) 682, 1306, 1515
TseFI GTSAC 3 cut(s) 352, 1512, 1798
Tsp45I GTSAC 3 cut(s) 352, 1512, 1798
TspDTI ATGAA 5 cut(s) 992, 1490, 1550, 1968, 2067
TspGWI ACGGA 4 cut(s) 340, 341, 431, 831
TspRI CASTG 3 cut(s) 682, 1306, 1515
VpaK11BI GGWCC 2 cut(s) 124, 331
XapI RAATTY 2 cut(s) 787, 1332
XmiI GTMKAC 3 cut(s) 528, 1649, 1962
XspI CTAG 2 cut(s) 687, 1203
ZrmI AGTACT 1 cut(s) 937
Zsp2I ATGCAT 1 cut(s) 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.