Rw7G001870

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011787
Physical Location & Seq
Forward (+)
1547472 .. 1549831
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001870.1

Sequence Viewer

Length: 2049 bp
ATGGATCCACCGCCTCTCCCACCGCTTCCGGCCACCACGGCCGCCACGCAACTCGGCTACCCCGATTCACTCGACTCCTCCCCCCGATCCCGCAATGCCGACTCTTCTTGGGACGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTGGTCGCCGAACGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAACGGCCGCCCCTTCTCTCTCAAGTACCAGCTCCCGACGGAGGACCTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTCGACAACATGGTGGAGGAGTACGACCGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTCCGTCTCTTTCTCTTCTTCGGCAAACCGGACACGGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCCGACTCGGCGGCCACCATGGACTGCTTGTTGACCGGCGGGAGTGATTCTTGCAACGACTTGGAGGCGGGCGGCGGCGGCGATGGAAAACCTAGTGTGGTGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCCTTTGTGGAGAGCAATACGTCGTCCTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCAAATTTGCCGCCAATTAGGGTTAGAATGGACGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCGGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTTCTGCTGCACCAACAATGGTGGCTGCTGCTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCTGGTCAGAATGTGCCTGTGGGTTTTCGAAAGCCGCCTTTGCCAATGCCATTGCAGCCGCAGCATTTTAAGCAGGGAGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCAGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCTCTGTCGAAACCAATGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTCCTGTTAGCCCGAATACAACAAGTGATGCTTCGGATCAGGGTTCCCGAAACCAAGTTCAACAAGTTCAGGATTCAGGGTACAGTGTGCCTCCGCAATCAGATCAACAACAGCAAATTCAGCTTCAGCAACAACAGCAGCAACAGCAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAATTTGTCCATGCAAGCGCGCAATATATGAATATTCAGCACCCTGCTACTGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAGTATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGATGGCTCTGCAGTCTACAATAGCTGATCCAGCTGGTGTTGCCTCAACCCGCATTACATCCCAAAACCCTGCTGCTTACAAGGACACTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTGTGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCAATATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCGTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCGATGACTCCAGCGGCCGCCGCAGCAGCAGCAGCAATGTCAGATGAATCAAAGCAGGTAGCCGCATACTCAGCAGCAGATCAAAGCCACACACAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

682

Amino Acids

73.25

Weight (kDa)

4.99

Isoelectric Point (pI)

70.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 65 - 155 1.8e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1705
Acc36I ACCTGC 2 cut(s) 1705, 1993
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 3 cut(s) 537, 1619, 1902
AccII CGCG 4 cut(s) 445, 542, 568, 1436
AclWI GGATC 6 cut(s) 12, 81, 384, 974, 1242, 1625
AcoI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 807, 1962
AcsI RAATTY 2 cut(s) 769, 1314
AcuI CTGAAG 1 cut(s) 1307
AcyI GRCGYC 4 cut(s) 114, 141, 488, 513
AfaI GTAC 8 cut(s) 289, 323, 401, 919, 1157, 1280, 1490, 1871
AfiI CCNNNNNNNGG 3 cut(s) 337, 528, 1466
AgsI TTSAA 4 cut(s) 433, 683, 1175, 1259
AhdI GACNNNNNGTC 1 cut(s) 517
Alw21I GWGCWC 1 cut(s) 1201
Alw26I GTCTC 2 cut(s) 218, 458
AlwI GGATC 6 cut(s) 12, 81, 384, 974, 1242, 1625
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 9 cut(s) 30, 39, 172, 301, 498, 588, 708, 807, 1962
ApoI RAATTY 2 cut(s) 769, 1314
ArsI GACNNNNNNTTYG 2 cut(s) 1127, 1159
AspLEI GCGC 4 cut(s) 143, 544, 1436, 1438
AspS9I GGNCC 3 cut(s) 133, 340, 498
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 6 cut(s) 355, 962, 1086, 1476, 1526, 1686
AsuII TTCGAA 1 cut(s) 1015
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 133, 340
AxyI CCTNAGG 1 cut(s) 129
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 1201
BbsI GAAGAC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 1201
BccI CCATC 4 cut(s) 653, 1184, 1519, 1600
BceAI ACGGC 7 cut(s) 54, 184, 316, 412, 421, 498, 565
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciVI GTATCC 1 cut(s) 1569
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 218, 458
BfaI CTAG 2 cut(s) 669, 1185
BfmI CTRYAG 2 cut(s) 1355, 1613
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 2 cut(s) 1705, 1993
BfuI GTATCC 1 cut(s) 1569
BglI GCCNNNNNGGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 2 cut(s) 133, 340
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 133, 340, 498
BmiI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1243
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 6 cut(s) 552, 1216, 1408, 1563, 1822, 1924
BpiI GAAGAC 1 cut(s) 738
BpmI CTGGAG 3 cut(s) 927, 1388, 1941
Bpu14I TTCGAA 1 cut(s) 1015
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 1949
BsaHI GRCGYC 4 cut(s) 114, 141, 488, 513
BsaJI CCNNGG 4 cut(s) 36, 136, 492, 594
BsaWI WCCGGW 2 cut(s) 475, 1096
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 528, 1466
Bse118I RCCGGY 2 cut(s) 611, 832
Bse1I ACTGG 3 cut(s) 1471, 1478, 1562
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 6 cut(s) 100, 1037, 1122, 1830, 1928, 1989
BseCI ATCGAT 1 cut(s) 1949
BseDI CCNNGG 4 cut(s) 36, 136, 492, 594
BseGI GGATG 4 cut(s) 177, 985, 1661, 1816
BseLI CCNNNNNNNGG 3 cut(s) 337, 528, 1466
BseMI GCAATG 6 cut(s) 100, 1037, 1122, 1830, 1928, 1989
BseMII CTCAG 5 cut(s) 143, 872, 954, 1838, 2031
BseNI ACTGG 3 cut(s) 1471, 1478, 1562
BsePI GCGCGC 1 cut(s) 1434
BseRI GAGGAG 9 cut(s) 67, 244, 284, 403, 406, 410, 860, 960, 1740
BseX3I CGGCCG 3 cut(s) 39, 301, 1962
BseYI CCCAGC 1 cut(s) 1911
BsgI GTGCAG 3 cut(s) 181, 839, 861
Bsh1236I CGCG 4 cut(s) 445, 542, 568, 1436
Bsh1285I CGRYCG 4 cut(s) 42, 304, 406, 1965
BshFI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 710, 809, 1964
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 1949
BsiEI CGRYCG 4 cut(s) 42, 304, 406, 1965
BsiHKAI GWGCWC 1 cut(s) 1201
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 7 cut(s) 29, 138, 476, 612, 810, 833, 1097
BsiWI CGTACG 1 cut(s) 1869
BslFI GGGAC 2 cut(s) 119, 125
BslI CCNNNNNNNGG 3 cut(s) 337, 528, 1466
BsmAI GTCTC 2 cut(s) 218, 458
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 119, 125
BsmI GAATGC 1 cut(s) 691
BsnI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 710, 809, 1964
BsoBI CYCGRG 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 1015
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp143I GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
Bsp19I CCATGG 2 cut(s) 492, 594
BspANI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 710, 809, 1964
BspCNI CTCAG 5 cut(s) 142, 871, 953, 1837, 2030
BspDI ATCGAT 1 cut(s) 1949
BspFNI CGCG 4 cut(s) 445, 542, 568, 1436
BspLI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1243
BspMAI CTGCAG 1 cut(s) 1617
BspMI ACCTGC 2 cut(s) 1705, 1993
BspPI GGATC 6 cut(s) 12, 81, 384, 974, 1242, 1625
BspT104I TTCGAA 1 cut(s) 1015
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 6 cut(s) 100, 1037, 1122, 1830, 1928, 1989
BsrFI RCCGGY 2 cut(s) 611, 832
BsrI ACTGG 3 cut(s) 1471, 1478, 1562
BssAI RCCGGY 2 cut(s) 611, 832
BssECI CCNNGG 4 cut(s) 36, 136, 492, 594
BssHII GCGCGC 1 cut(s) 1434
BssMI GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
BssNAI GTATAC 1 cut(s) 1903
BssNI GRCGYC 4 cut(s) 114, 141, 488, 513
BssT1I CCWWGG 2 cut(s) 492, 594
Bst1107I GTATAC 1 cut(s) 1903
Bst4CI ACNGT 3 cut(s) 1283, 1488, 1587
Bst6I CTCTTC 2 cut(s) 109, 467
BstACI GRCGYC 4 cut(s) 114, 141, 488, 513
BstAPI GCANNNNNTGC 1 cut(s) 1351
BstBI TTCGAA 1 cut(s) 1015
BstC8I GCNNGC 6 cut(s) 191, 646, 834, 1088, 1432, 1436
BstDEI CTNAG 5 cut(s) 129, 858, 940, 1824, 2017
BstDSI CCRYGG 3 cut(s) 36, 492, 594
BstF5I GGATG 4 cut(s) 177, 985, 1661, 1816
BstFNI CGCG 4 cut(s) 445, 542, 568, 1436
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 143, 544, 1436, 1438
BstKTI GATC 9 cut(s) 7, 89, 379, 969, 1237, 1303, 1546, 1633, 2029
BstMAI GTCTC 2 cut(s) 218, 458
BstMBI GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
BstMCI CGRYCG 4 cut(s) 42, 304, 406, 1965
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 2 cut(s) 1355, 1613
BstUI CGCG 4 cut(s) 445, 542, 568, 1436
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 2 cut(s) 4, 376
BstYI RGATCY 2 cut(s) 4, 376
BstZ17I GTATAC 1 cut(s) 1903
BstZI CGGCCG 3 cut(s) 39, 301, 1962
Bsu15I ATCGAT 1 cut(s) 1949
Bsu36I CCTNAGG 1 cut(s) 129
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 710, 809, 1964
BsuTUI ATCGAT 1 cut(s) 1949
BtgI CCRYGG 3 cut(s) 36, 492, 594
BtgZI GCGATG 2 cut(s) 672, 1497
BtsCI GGATG 4 cut(s) 177, 985, 1661, 1816
BtsIMutI CAGTG 2 cut(s) 1288, 1485
BveI ACCTGC 2 cut(s) 1705, 1993
Cac8I GCNNGC 6 cut(s) 191, 646, 834, 1088, 1432, 1436
CciNI GCGGCCGC 1 cut(s) 1962
CfoI GCGC 4 cut(s) 143, 544, 1436, 1438
Cfr10I RCCGGY 2 cut(s) 611, 832
Cfr13I GGNCC 3 cut(s) 133, 340, 498
ClaI ATCGAT 1 cut(s) 1949
CseI GACGC 5 cut(s) 122, 477, 521, 548, 1116
Csp6I GTAC 8 cut(s) 288, 322, 400, 918, 1156, 1279, 1489, 1870
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 6 cut(s) 157, 388, 493, 595, 1427, 1892
CviQI GTAC 8 cut(s) 288, 322, 400, 918, 1156, 1279, 1489, 1870
DdeI CTNAG 5 cut(s) 129, 858, 940, 1824, 2017
DinI GGCGCC 1 cut(s) 142
DpnI GATC 9 cut(s) 6, 88, 378, 968, 1236, 1302, 1545, 1632, 2028
DpnII GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
DriI GACNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 807, 1962
EagI CGGCCG 3 cut(s) 39, 301, 1962
Eam1104I CTCTTC 2 cut(s) 109, 467
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 2 cut(s) 109, 467
EciI GGCGGA 2 cut(s) 140, 409
Ecl136II GAGCTC 1 cut(s) 1199
EclXI CGGCCG 3 cut(s) 39, 301, 1962
Eco130I CCWWGG 2 cut(s) 492, 594
Eco24I GRGCYC 1 cut(s) 1201
Eco47I GGWCC 2 cut(s) 133, 340
Eco52I CGGCCG 3 cut(s) 39, 301, 1962
Eco53kI GAGCTC 1 cut(s) 1199
Eco57I CTGAAG 1 cut(s) 1307
Eco81I CCTNAGG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 1199
EcoO109I RGGNCCY 3 cut(s) 133, 340, 498
EcoT14I CCWWGG 2 cut(s) 492, 594
EcoT22I ATGCAT 1 cut(s) 1815
EcoT38I GRGCYC 1 cut(s) 1201
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 492, 594
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 6 cut(s) 160, 391, 496, 598, 1430, 1895
FalI AAGNNNNNCTT 2 cut(s) 1213, 1245
FaqI GGGAC 2 cut(s) 119, 125
FatI CATG 6 cut(s) 156, 387, 492, 594, 1426, 1891
FauI CCCGC 8 cut(s) 98, 194, 198, 284, 608, 637, 829, 1661
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 3 cut(s) 537, 1619, 1902
FokI GGATG 4 cut(s) 164, 992, 1648, 1803
FriOI GRGCYC 1 cut(s) 1201
FspBI CTAG 2 cut(s) 669, 1185
GlaI GCGC 4 cut(s) 142, 543, 1435, 1437
GsaI CCCAGC 1 cut(s) 1915
GsuI CTGGAG 3 cut(s) 927, 1388, 1941
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 710, 809, 1964
HapII CCGG 7 cut(s) 29, 138, 476, 612, 810, 833, 1097
HgaI GACGC 5 cut(s) 122, 477, 521, 548, 1116
HhaI GCGC 4 cut(s) 143, 544, 1436, 1438
Hin1I GRCGYC 4 cut(s) 114, 141, 488, 513
Hin1II CATG 6 cut(s) 160, 391, 496, 598, 1430, 1895
Hin6I GCGC 4 cut(s) 141, 542, 1434, 1436
HinP1I GCGC 4 cut(s) 141, 542, 1434, 1436
HincII GTYRAC 2 cut(s) 538, 609
HindII GTYRAC 2 cut(s) 538, 609
HindIII AAGCTT 1 cut(s) 865
HpaII CCGG 7 cut(s) 29, 138, 476, 612, 810, 833, 1097
HphI GGTGA 6 cut(s) 355, 962, 1086, 1476, 1526, 1686
Hpy166II GTNNAC 6 cut(s) 538, 609, 937, 1620, 1726, 1903
Hpy188III TCNNGA 8 cut(s) 331, 380, 683, 906, 942, 1163, 1245, 1268
Hpy8I GTNNAC 6 cut(s) 538, 609, 937, 1620, 1726, 1903
HpyAV CCTTC 7 cut(s) 319, 1101, 1779, 1830, 1851, 1896, 1944
HpyCH4III ACNGT 3 cut(s) 1283, 1488, 1587
HpyCH4IV ACGT 2 cut(s) 213, 728
HpyF3I CTNAG 5 cut(s) 129, 858, 940, 1824, 2017
HpySE526I ACGT 2 cut(s) 213, 728
Hsp92I GRCGYC 4 cut(s) 114, 141, 488, 513
Hsp92II CATG 6 cut(s) 160, 391, 496, 598, 1430, 1895
HspAI GCGC 4 cut(s) 141, 542, 1434, 1436
KasI GGCGCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 832
KroNI GCCGGC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 1543
Kzo9I GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
LmnI GCTCC 9 cut(s) 153, 278, 333, 453, 847, 908, 1204, 1392, 1510
LweI GCATC 6 cut(s) 552, 1216, 1408, 1563, 1822, 1924
MaeI CTAG 2 cut(s) 669, 1185
MaeII ACGT 2 cut(s) 213, 728
MaeIII GTNAC 4 cut(s) 361, 1482, 1738, 1799
MalI GATC 9 cut(s) 6, 88, 378, 968, 1236, 1302, 1545, 1632, 2028
MboI GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
MboII GAAGA 5 cut(s) 96, 454, 457, 738, 741
MflI RGATCY 2 cut(s) 4, 376
MhlI GDGCHC 1 cut(s) 1201
MluCI AATT 7 cut(s) 769, 780, 1314, 1418, 1539, 1754, 1790
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 8 cut(s) 68, 95, 220, 341, 533, 575, 1708, 1948
MmeI TCCRAC 4 cut(s) 288, 603, 1414, 1797
Mph1103I ATGCAT 1 cut(s) 1815
MroNI GCCGGC 1 cut(s) 832
MseI TTAA 1 cut(s) 1056
MspA1I CMGCKG 2 cut(s) 1637, 1961
MspI CCGG 7 cut(s) 29, 138, 476, 612, 810, 833, 1097
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 691
MvnI CGCG 4 cut(s) 445, 542, 568, 1436
NaeI GCCGGC 1 cut(s) 834
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 492, 594
NdeII GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
NgoMIV GCCGGC 1 cut(s) 832
NlaIII CATG 6 cut(s) 160, 391, 496, 598, 1430, 1895
NlaIV GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1243
NmeAIII GCCGAG 3 cut(s) 33, 142, 563
NmuCI GTSAC 3 cut(s) 361, 1482, 1738
NotI GCGGCCGC 1 cut(s) 1962
NsiI ATGCAT 1 cut(s) 1815
NspV TTCGAA 1 cut(s) 1015
PaqCI CACCTGC 1 cut(s) 1705
PauI GCGCGC 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 219, 378, 519, 749
PctI GAATGC 1 cut(s) 691
PdiI GCCGGC 1 cut(s) 834
PfeI GAWTC 6 cut(s) 65, 623, 704, 1100, 1271, 1994
Pfl23II CGTACG 1 cut(s) 1869
PleI GAGTC 8 cut(s) 68, 95, 220, 341, 532, 575, 1708, 1948
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 8 cut(s) 68, 95, 220, 341, 532, 575, 1708, 1948
PpuMI RGGWCCY 2 cut(s) 133, 340
Psp124BI GAGCTC 1 cut(s) 1201
Psp5II RGGWCCY 2 cut(s) 133, 340
PspFI CCCAGC 1 cut(s) 1911
PspLI CGTACG 1 cut(s) 1869
PspN4I GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1243
PspPI GGNCC 3 cut(s) 133, 340, 498
PspPPI RGGWCCY 2 cut(s) 133, 340
PstI CTGCAG 1 cut(s) 1617
PsuI RGATCY 2 cut(s) 4, 376
PteI GCGCGC 1 cut(s) 1434
PvuII CAGCTG 1 cut(s) 1637
RsaI GTAC 8 cut(s) 289, 323, 401, 919, 1157, 1280, 1490, 1871
RsaNI GTAC 8 cut(s) 288, 322, 400, 918, 1156, 1279, 1489, 1870
SacI GAGCTC 1 cut(s) 1201
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 1056
Sau3AI GATC 9 cut(s) 4, 86, 376, 966, 1234, 1300, 1543, 1630, 2026
Sau96I GGNCC 3 cut(s) 133, 340, 498
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 8 cut(s) 68, 95, 220, 341, 533, 575, 1708, 1948
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 1201
SfaNI GCATC 6 cut(s) 552, 1216, 1408, 1563, 1822, 1924
SfcI CTRYAG 2 cut(s) 1355, 1613
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 1015
SgrDI CGTCGACG 1 cut(s) 536
SinI GGWCC 2 cut(s) 133, 340
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 7 cut(s) 769, 780, 1314, 1418, 1539, 1754, 1790
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 1450
SspMI CTAG 2 cut(s) 669, 1185
SstI GAGCTC 1 cut(s) 1201
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 492, 594
TaaI ACNGT 3 cut(s) 1283, 1488, 1587
TaiI ACGT 2 cut(s) 216, 731
TaqI TCGA 9 cut(s) 72, 345, 381, 507, 537, 695, 1015, 1145, 1949
TasI AATT 7 cut(s) 769, 780, 1314, 1418, 1539, 1754, 1790
TatI WGTACW 2 cut(s) 917, 1155
TfiI GAWTC 6 cut(s) 65, 623, 704, 1100, 1271, 1994
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 2 cut(s) 1288, 1485
TseFI GTSAC 3 cut(s) 361, 1482, 1738
Tsp45I GTSAC 3 cut(s) 361, 1482, 1738
TspDTI ATGAA 5 cut(s) 974, 1460, 1520, 1908, 2007
TspGWI ACGGA 4 cut(s) 349, 350, 440, 813
TspRI CASTG 2 cut(s) 1288, 1485
VpaK11BI GGWCC 2 cut(s) 133, 340
XapI RAATTY 2 cut(s) 769, 1314
XmiI GTMKAC 3 cut(s) 537, 1619, 1902
XspI CTAG 2 cut(s) 669, 1185
ZrmI AGTACT 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 1815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.