Rh7BG022800

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011787
Physical Location & Seq
Forward (+)
1619715 .. 1623603
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG022800.1

Sequence Viewer

Length: 2103 bp
ATGGATCCGCCGCCTCTCCCACCACTTCCGGCCACCGCCGCTGCCACCACGGCCGCCACGCAACTCGGCTACTCCGATTCACTCGACTCCTCCCCCCGATCCCGAAATGCCGACTCTTCTTGGGACGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTCGTCGCCGACCGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAACGGCCGCCCCTTCTCTCTCAAGTACCAGCTCCCGACGGAGGAACTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTCGACAACATGGTGGAGGAGTACGACCGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTCCGCCTCTTTCTCTTCTTCGGCAAACCGGAGACGGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCCGACTCGGCGGCAACCATGGACTGCTTGTTGACCGGCGGGAGTGATTCTTGCAACGACCTGGAGGCGGCGGCGAGTCAAGGCGACTTCTTTGGGGATCATAACAGTGGAAAACCTAGTGTGGTGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCGTTTGTGGAGAGCAATACGTCGTCGTCGTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCGAATTTGCCGCCAATTAGGGTTAGAATGGACGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCTGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTGCTACTGCACCAACAATGGTGGCTGCTGCTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCGGGTCAGAATGTGCCTGTGGGGTTTCGAAAGCCGCCTTTGCCGATGCCATTGCAGCCGCAGCATTTTAAGCAGGGTGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCGGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCTCTGTCGAAACCAATGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTCCTGTTAGCCCGAATACAACAAGTGATGCTTCGGATCAGGGTTCCCGAAACCAAGTTCAGGATTCAGGGTACAGTGTGCCTCCGCAATTAGATCAACAACAGCAAAATCAGCTTCAGCAACAACAGCAGCAGCAACAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAGCAGCAGCAACAATTTGTCCATGCAAGCGCGCAATATATGAATATCCAGCACCCTGCTACTGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAATATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGATGGCTCTGCAGTCTACAATAGCTGATCCAGCTGGTGTTGCCTCGACGACCCGCATTACATCCCAAAACCCTGCTGCTTACAAGGACAGTATCCCAACCATTTATCCCCCAAAGACAGCTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTGTGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCATTATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCTTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCCATGACTCCAGCAGCCGCCGCCGCAGCAGTAGCAATGTCAGATGAATCAAAGCAGAAAAACCAGATGCAGATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

700

Amino Acids

75.49

Weight (kDa)

5.16

Isoelectric Point (pI)

70.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 69 - 159 3.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1780
AasI GACNNNNNNGTC 1 cut(s) 499
Acc36I ACCTGC 1 cut(s) 1780
AccB1I GGYRCC 1 cut(s) 152
AccI GTMKAC 3 cut(s) 549, 1661, 1977
AccII CGCG 4 cut(s) 457, 554, 580, 1478
AclWI GGATC 7 cut(s) 12, 93, 396, 693, 1013, 1281, 1667
AcoI YGGCCR 5 cut(s) 30, 51, 184, 313, 846
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 1 cut(s) 1337
AcyI GRCGYC 4 cut(s) 126, 153, 500, 525
AfaI GTAC 7 cut(s) 301, 335, 413, 958, 1196, 1310, 1532
AfiI CCNNNNNNNGG 5 cut(s) 349, 540, 655, 1297, 1508
AgsI TTSAA 3 cut(s) 445, 719, 1214
AhdI GACNNNNNGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 648
AjuI GAANNNNNNNTTGG 2 cut(s) 1750, 1782
Alw21I GWGCWC 1 cut(s) 1240
Alw26I GTCTC 2 cut(s) 230, 485
AlwI GGATC 7 cut(s) 12, 93, 396, 693, 1013, 1281, 1667
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 6 cut(s) 30, 51, 184, 313, 510, 846
ApoI RAATTY 1 cut(s) 808
ArsI GACNNNNNNTTYG 2 cut(s) 1166, 1198
AspLEI GCGC 4 cut(s) 155, 556, 1478, 1480
AspS9I GGNCC 2 cut(s) 145, 510
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 6 cut(s) 367, 1001, 1125, 1518, 1568, 1761
AsuII TTCGAA 1 cut(s) 1054
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 1 cut(s) 145
AxyI CCTNAGG 1 cut(s) 141
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 1240
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 1 cut(s) 1240
BccI CCATC 3 cut(s) 1223, 1561, 1642
BceAI ACGGC 7 cut(s) 66, 196, 328, 424, 433, 510, 577
BcgI CGANNNNNNTGC 4 cut(s) 232, 266, 1060, 1094
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 1 cut(s) 1748
BclI TGATCA 1 cut(s) 1585
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 230, 485
BfaI CTAG 2 cut(s) 705, 1224
BfmI CTRYAG 2 cut(s) 1385, 1655
BfoI RGCGCY 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 1780
BfuI GTATCC 1 cut(s) 1748
BglI GCCNNNNNGGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 958
Bme1390I CCNGG 2 cut(s) 150, 650
Bme18I GGWCC 1 cut(s) 145
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 145, 510
BmiI GGNNCC 6 cut(s) 6, 147, 154, 461, 511, 1282
BmrFI CCNGG 2 cut(s) 150, 650
BmsI GCATC 8 cut(s) 564, 1062, 1255, 1438, 1605, 1897, 1999, 2079
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 4 cut(s) 671, 966, 1418, 2016
Bpu14I TTCGAA 1 cut(s) 1054
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 2093
BsaHI GRCGYC 4 cut(s) 126, 153, 500, 525
BsaJI CCNNGG 4 cut(s) 48, 148, 504, 606
BsaWI WCCGGW 2 cut(s) 487, 1135
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 540, 655, 1297, 1508
Bse118I RCCGGY 2 cut(s) 623, 871
Bse1I ACTGG 3 cut(s) 1513, 1520, 1604
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 5 cut(s) 1076, 1161, 1905, 2003, 2064
Bse8I GATNNNNATC 1 cut(s) 2093
BseBI CCWGG 1 cut(s) 650
BseDI CCNNGG 4 cut(s) 48, 148, 504, 606
BseGI GGATG 4 cut(s) 189, 1024, 1706, 1891
BseJI GATNNNNATC 1 cut(s) 2093
BseLI CCNNNNNNNGG 5 cut(s) 349, 540, 655, 1297, 1508
BseMI GCAATG 5 cut(s) 1076, 1161, 1905, 2003, 2064
BseMII CTCAG 4 cut(s) 155, 911, 993, 1913
BseNI ACTGG 3 cut(s) 1513, 1520, 1604
BsePI GCGCGC 1 cut(s) 1476
BseRI GAGGAG 9 cut(s) 79, 256, 296, 415, 418, 422, 899, 999, 1815
BseX3I CGGCCG 2 cut(s) 51, 313
BseYI CCCAGC 2 cut(s) 875, 1986
BsgI GTGCAG 3 cut(s) 193, 878, 900
Bsh1236I CGCG 4 cut(s) 457, 554, 580, 1478
Bsh1285I CGRYCG 4 cut(s) 54, 259, 316, 418
BshFI GGCC 6 cut(s) 32, 53, 186, 315, 512, 848
BshNI GGYRCC 1 cut(s) 152
BsiEI CGRYCG 4 cut(s) 54, 259, 316, 418
BsiHKAI GWGCWC 1 cut(s) 1240
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 7 cut(s) 29, 150, 488, 624, 849, 872, 1136
BslFI GGGAC 2 cut(s) 131, 137
BslI CCNNNNNNNGG 5 cut(s) 349, 540, 655, 1297, 1508
BsmAI GTCTC 2 cut(s) 230, 485
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 2 cut(s) 131, 137
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 6 cut(s) 32, 53, 186, 315, 512, 848
BsoBI CYCGRG 1 cut(s) 565
Bsp119I TTCGAA 1 cut(s) 1054
Bsp1286I GDGCHC 1 cut(s) 1240
Bsp19I CCATGG 2 cut(s) 504, 606
BspANI GGCC 6 cut(s) 32, 53, 186, 315, 512, 848
BspCNI CTCAG 4 cut(s) 154, 910, 992, 1912
BspFNI CGCG 4 cut(s) 457, 554, 580, 1478
BspLI GGNNCC 6 cut(s) 6, 147, 154, 461, 511, 1282
BspMAI CTGCAG 1 cut(s) 1659
BspMI ACCTGC 1 cut(s) 1780
BspPI GGATC 7 cut(s) 12, 93, 396, 693, 1013, 1281, 1667
BspT104I TTCGAA 1 cut(s) 1054
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 5 cut(s) 1076, 1161, 1905, 2003, 2064
BsrFI RCCGGY 2 cut(s) 623, 871
BsrI ACTGG 3 cut(s) 1513, 1520, 1604
BssAI RCCGGY 2 cut(s) 623, 871
BssECI CCNNGG 4 cut(s) 48, 148, 504, 606
BssHII GCGCGC 1 cut(s) 1476
BssNAI GTATAC 1 cut(s) 1978
BssNI GRCGYC 4 cut(s) 126, 153, 500, 525
BssT1I CCWWGG 2 cut(s) 504, 606
Bst1107I GTATAC 1 cut(s) 1978
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 5 cut(s) 695, 1313, 1530, 1629, 1736
Bst6I CTCTTC 2 cut(s) 121, 479
BstACI GRCGYC 4 cut(s) 126, 153, 500, 525
BstBI TTCGAA 1 cut(s) 1054
BstC8I GCNNGC 5 cut(s) 203, 873, 1127, 1474, 1478
BstDEI CTNAG 4 cut(s) 141, 897, 979, 1899
BstDSI CCRYGG 3 cut(s) 48, 504, 606
BstF5I GGATG 4 cut(s) 189, 1024, 1706, 1891
BstFNI CGCG 4 cut(s) 457, 554, 580, 1478
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 4 cut(s) 155, 556, 1478, 1480
BstMAI GTCTC 2 cut(s) 230, 485
BstMCI CGRYCG 4 cut(s) 54, 259, 316, 418
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 148, 648
BstSFI CTRYAG 2 cut(s) 1385, 1655
BstUI CGCG 4 cut(s) 457, 554, 580, 1478
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 2 cut(s) 4, 388
BstYI RGATCY 2 cut(s) 4, 388
BstZ17I GTATAC 1 cut(s) 1978
BstZI CGGCCG 2 cut(s) 51, 313
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 1 cut(s) 1748
BsuRI GGCC 6 cut(s) 32, 53, 186, 315, 512, 848
BtgI CCRYGG 3 cut(s) 48, 504, 606
BtgZI GCGATG 1 cut(s) 1539
BtsCI GGATG 4 cut(s) 189, 1024, 1706, 1891
BtsIMutI CAGTG 3 cut(s) 700, 1318, 1527
BveI ACCTGC 1 cut(s) 1780
Cac8I GCNNGC 5 cut(s) 203, 873, 1127, 1474, 1478
CfoI GCGC 4 cut(s) 155, 556, 1478, 1480
Cfr10I RCCGGY 2 cut(s) 623, 871
Cfr13I GGNCC 2 cut(s) 145, 510
CseI GACGC 5 cut(s) 134, 489, 533, 560, 1155
Csp6I GTAC 7 cut(s) 300, 334, 412, 957, 1195, 1309, 1531
CspCI CAANNNNNGTGG 2 cut(s) 909, 944
CviAII CATG 7 cut(s) 169, 400, 505, 607, 1469, 1967, 2026
CviQI GTAC 7 cut(s) 300, 334, 412, 957, 1195, 1309, 1531
DdeI CTNAG 4 cut(s) 141, 897, 979, 1899
DinI GGCGCC 1 cut(s) 154
DrdI GACNNNNNNGTC 1 cut(s) 499
DriI GACNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 5 cut(s) 30, 51, 184, 313, 846
EagI CGGCCG 2 cut(s) 51, 313
Eam1104I CTCTTC 2 cut(s) 121, 479
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 2 cut(s) 121, 479
EciI GGCGGA 3 cut(s) 152, 421, 452
Ecl136II GAGCTC 1 cut(s) 1238
EclXI CGGCCG 2 cut(s) 51, 313
Eco130I CCWWGG 2 cut(s) 504, 606
Eco24I GRGCYC 1 cut(s) 1240
Eco47I GGWCC 1 cut(s) 145
Eco52I CGGCCG 2 cut(s) 51, 313
Eco53kI GAGCTC 1 cut(s) 1238
Eco57I CTGAAG 1 cut(s) 1337
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 1238
EcoO109I RGGNCCY 2 cut(s) 145, 510
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 504, 606
EcoT22I ATGCAT 1 cut(s) 1890
EcoT38I GRGCYC 1 cut(s) 1240
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 504, 606
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 7 cut(s) 172, 403, 508, 610, 1472, 1970, 2029
FalI AAGNNNNNCTT 2 cut(s) 1252, 1284
FaqI GGGAC 2 cut(s) 131, 137
FatI CATG 7 cut(s) 168, 399, 504, 606, 1468, 1966, 2025
FauI CCCGC 5 cut(s) 206, 210, 296, 620, 1706
FbaI TGATCA 1 cut(s) 1585
FblI GTMKAC 3 cut(s) 549, 1661, 1977
FokI GGATG 4 cut(s) 176, 1031, 1693, 1878
FriOI GRGCYC 1 cut(s) 1240
FspBI CTAG 2 cut(s) 705, 1224
GlaI GCGC 4 cut(s) 154, 555, 1477, 1479
GsaI CCCAGC 2 cut(s) 879, 1990
GsuI CTGGAG 4 cut(s) 671, 966, 1418, 2016
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 6 cut(s) 32, 53, 186, 315, 512, 848
HapII CCGG 7 cut(s) 29, 150, 488, 624, 849, 872, 1136
HgaI GACGC 5 cut(s) 134, 489, 533, 560, 1155
HhaI GCGC 4 cut(s) 155, 556, 1478, 1480
Hin1I GRCGYC 4 cut(s) 126, 153, 500, 525
Hin1II CATG 7 cut(s) 172, 403, 508, 610, 1472, 1970, 2029
Hin6I GCGC 4 cut(s) 153, 554, 1476, 1478
HinP1I GCGC 4 cut(s) 153, 554, 1476, 1478
HincII GTYRAC 2 cut(s) 550, 621
HindII GTYRAC 2 cut(s) 550, 621
HindIII AAGCTT 1 cut(s) 904
HpaII CCGG 7 cut(s) 29, 150, 488, 624, 849, 872, 1136
HphI GGTGA 6 cut(s) 367, 1001, 1125, 1518, 1568, 1761
Hpy166II GTNNAC 6 cut(s) 550, 621, 976, 1662, 1801, 1978
Hpy188III TCNNGA 9 cut(s) 102, 343, 392, 719, 945, 981, 1202, 1284, 1298
Hpy8I GTNNAC 6 cut(s) 550, 621, 976, 1662, 1801, 1978
HpyAV CCTTC 7 cut(s) 331, 1140, 1854, 1905, 1926, 1971, 2019
HpyCH4III ACNGT 5 cut(s) 695, 1313, 1530, 1629, 1736
HpyCH4IV ACGT 2 cut(s) 225, 764
HpyF3I CTNAG 4 cut(s) 141, 897, 979, 1899
HpySE526I ACGT 2 cut(s) 225, 764
Hsp92I GRCGYC 4 cut(s) 126, 153, 500, 525
Hsp92II CATG 7 cut(s) 172, 403, 508, 610, 1472, 1970, 2029
HspAI GCGC 4 cut(s) 153, 554, 1476, 1478
KasI GGCGCC 1 cut(s) 152
KroI GCCGGC 1 cut(s) 871
KroNI GCCGGC 1 cut(s) 873
Ksp22I TGATCA 1 cut(s) 1585
LmnI GCTCC 9 cut(s) 165, 290, 345, 465, 886, 947, 1243, 1422, 1552
LweI GCATC 8 cut(s) 564, 1062, 1255, 1438, 1605, 1897, 1999, 2079
MaeI CTAG 2 cut(s) 705, 1224
MaeII ACGT 2 cut(s) 225, 764
MaeIII GTNAC 4 cut(s) 373, 1524, 1813, 1874
MboII GAAGA 5 cut(s) 108, 466, 469, 777, 780
MflI RGATCY 2 cut(s) 4, 388
MhlI GDGCHC 1 cut(s) 1240
MluCI AATT 8 cut(s) 808, 819, 1325, 1460, 1581, 1829, 1865, 2098
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 9 cut(s) 80, 107, 232, 353, 545, 587, 673, 1783, 2023
MmeI TCCRAC 4 cut(s) 300, 615, 1444, 1872
Mph1103I ATGCAT 1 cut(s) 1890
MroNI GCCGGC 1 cut(s) 871
MseI TTAA 1 cut(s) 1095
MslI CAYNNNNRTG 1 cut(s) 693
MspA1I CMGCKG 2 cut(s) 41, 1679
MspI CCGG 7 cut(s) 29, 150, 488, 624, 849, 872, 1136
MspR9I CCNGG 2 cut(s) 150, 650
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 1 cut(s) 650
MvnI CGCG 4 cut(s) 457, 554, 580, 1478
NaeI GCCGGC 1 cut(s) 873
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 2 cut(s) 504, 606
NgoMIV GCCGGC 1 cut(s) 871
NlaIII CATG 7 cut(s) 172, 403, 508, 610, 1472, 1970, 2029
NlaIV GGNNCC 6 cut(s) 6, 147, 154, 461, 511, 1282
NmeAIII GCCGAG 3 cut(s) 45, 154, 575
NmuCI GTSAC 3 cut(s) 373, 1524, 1813
NsiI ATGCAT 1 cut(s) 1890
NspV TTCGAA 1 cut(s) 1054
PaqCI CACCTGC 1 cut(s) 1780
PauI GCGCGC 1 cut(s) 1476
PcsI WCGNNNNNNNCGW 8 cut(s) 72, 231, 390, 531, 743, 770, 779, 788
PctI GAATGC 1 cut(s) 727
PdiI GCCGGC 1 cut(s) 873
PfeI GAWTC 6 cut(s) 77, 635, 740, 1139, 1301, 2069
PleI GAGTC 9 cut(s) 80, 107, 232, 353, 544, 587, 672, 1783, 2023
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 9 cut(s) 80, 107, 232, 353, 544, 587, 672, 1783, 2023
PpuMI RGGWCCY 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 1240
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 648
PspFI CCCAGC 2 cut(s) 875, 1986
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 6 cut(s) 6, 147, 154, 461, 511, 1282
PspPI GGNCC 2 cut(s) 145, 510
PspPPI RGGWCCY 1 cut(s) 145
PstI CTGCAG 1 cut(s) 1659
PsuI RGATCY 2 cut(s) 4, 388
PteI GCGCGC 1 cut(s) 1476
PvuII CAGCTG 1 cut(s) 1679
RsaI GTAC 7 cut(s) 301, 335, 413, 958, 1196, 1310, 1532
RsaNI GTAC 7 cut(s) 300, 334, 412, 957, 1195, 1309, 1531
RseI CAYNNNNRTG 1 cut(s) 693
SacI GAGCTC 1 cut(s) 1240
SalI GTCGAC 1 cut(s) 548
SaqAI TTAA 1 cut(s) 1095
Sau96I GGNCC 2 cut(s) 145, 510
ScaI AGTACT 1 cut(s) 958
SchI GAGTC 9 cut(s) 80, 107, 232, 353, 545, 587, 673, 1783, 2023
ScrFI CCNGG 2 cut(s) 150, 650
SduI GDGCHC 1 cut(s) 1240
SfaNI GCATC 8 cut(s) 564, 1062, 1255, 1438, 1605, 1897, 1999, 2079
SfcI CTRYAG 2 cut(s) 1385, 1655
SfoI GGCGCC 1 cut(s) 154
SfuI TTCGAA 1 cut(s) 1054
SgrDI CGTCGACG 1 cut(s) 548
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 8 cut(s) 808, 819, 1325, 1460, 1581, 1829, 1865, 2098
SspDI GGCGCC 1 cut(s) 152
SspMI CTAG 2 cut(s) 705, 1224
SstI GAGCTC 1 cut(s) 1240
StyD4I CCNGG 2 cut(s) 148, 648
StyI CCWWGG 2 cut(s) 504, 606
TaaI ACNGT 5 cut(s) 695, 1313, 1530, 1629, 1736
TaiI ACGT 2 cut(s) 228, 767
TasI AATT 8 cut(s) 808, 819, 1325, 1460, 1581, 1829, 1865, 2098
TatI WGTACW 2 cut(s) 956, 1194
TfiI GAWTC 6 cut(s) 77, 635, 740, 1139, 1301, 2069
Tru1I TTAA 1 cut(s) 1095
Tru9I TTAA 1 cut(s) 1095
TscAI CASTG 3 cut(s) 700, 1318, 1527
TseFI GTSAC 3 cut(s) 373, 1524, 1813
Tsp45I GTSAC 3 cut(s) 373, 1524, 1813
TspDTI ATGAA 5 cut(s) 1013, 1502, 1562, 1983, 2082
TspGWI ACGGA 3 cut(s) 361, 362, 852
TspRI CASTG 3 cut(s) 700, 1318, 1527
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 1 cut(s) 808
XmiI GTMKAC 3 cut(s) 549, 1661, 1977
XspI CTAG 2 cut(s) 705, 1224
ZrmI AGTACT 1 cut(s) 958
Zsp2I ATGCAT 1 cut(s) 1890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.