Prupe.5G225400_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0011787
Physical Location & Seq
Reverse (-)
17419051 .. 17422725
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G225400.3

Sequence Viewer

Length: 2118 bp
ATGGATCCACCACCTCTCCCACCTCTTCCCACCACAATCACCACCACAGCCATGGCAGCCACCAGCATGCAACCCAACTACCCTGACTCAGTTGACTCGTCCCCAAGGTCTCGAAATGTCGACGCATGGGATGAGCCTCTCCCCCAAATCCCAGGCGCCAAGCTTCGCCTCATGTGCAGCTATGGCGGCCACATCATCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAAACCCGCATTGTACTAACCGAACGCAGCTCCTCACTCTCCGACCTCTGCTCCCGCCTCTCCCGGACCCTCCTCCATGGCCGGCCTTTCTCTCTCAAGTACCAGCTACCAAGCGAAGACCTTGACTCACTCATCTCAGTCACCACCGACGAAGATCTCGACAACATGATCGAAGAATACGACCGCACGGCTTCCACCTCTCCTTTGAAGCCTTCGCGGCTCCGATTGTTTCTCTTCTTTGCCAAGCCTGAAACTGCTGCGTCCATGGGGGCTTTGCTTGATGATGCAAAGTCCGAGTCTTGGTTCGTCGATGCGCTTAATGGGTCGGGTCTTATTCCCAGAAATCTCTCGGACTCGGCCACCATGGATTGCTTGTTGACTGGGAGTGAGAATTCTTGTAACGATTTGGAGGCTCAAGGTGACTTCTTTGGTGACAACAACAAACAGGGTATTAATGATGTGATGAAGAATGTTAATAATGTAGTACATGATGTGCATTCGATCAATCAAGAGTCACCCTTTGTGGAGAATAGTAGTTCGTCTTTTGGGTCGTCTACTTCATCGCCTTGCTTGTCTAATTTGCCTTCGATTCGGGTTCGTATGGATGACAATGGGGCTAGAGTGATGCAGAATGGTCACAAGGCTATTGGGATAGAGGAACAGTTTGCTCAAATGAGTACTGGTTTTGCTGCTGCTGCACCATCATTGGCTGCTAATTCTGGGGCTTTGCCGCCATCTGGTACTCAGATGATGAATGCTATGCCTTTTTCTGGAGAAAATGTGAACAGGGTCATCTCATCAGATGATGAGAGATCGTCGGATCAGAGTGTGCCAGTTGGGTTTCGTAAGCCTCCTCTGCCATTACCTTTGCAGCCTTTGCAAGTTAATAAGGCTGCTGGTGGTGGTGGAGGAGGTTATAGTTTGCCTTCACCCGATTCAGTTGCAAGTGATAGTAGTATTGCATCTGCAAACTCGCTCTCCAAACCAATGTACTACCAAGAGCAGATCCAAGTTGCATCTAGGGAAAACACCAGAGGTCCTGTTAGTCCAAATACAAACAGCGATGCTTCCGATCTGGGTTCCCAAAGCCAAGTTCAACAAGTTCAGGATTCAGGGTACACCATGCCTCCACAATCTGATCAACAACAGCAGCAGCAACAACAACAACAACAGCAGCAGCAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAACAACAATTTGTCCATGCCAACACTCATTATATGCACCACCCTGCCACAGGTCAAGCGCCAATGCAATCTTACTACACGATGTATGCTCCACCACCACAGCAACAGCTTCATCACCAGCTTGATCATCAGCAGTATCCAGTCTATTTGATGCCTGTTGGCCAGTCACAACCATACAACATGCCTTTGCAACCTAACATGGCTGATACCACTGCTGTGGCCTCAAGCCGCTTAACATCCCAAAATCCTGCCTCCGCTGTTTACAAGGATAGTATTCCACCCATTTATCCCACAAAGACAACTTCACCTACTATGCCTGAAATGTCTGCAAGCGTTTATAAAACGATTGTTCCACAAACTCCGTCATTAGTCCAAGTTCCTTCTAACCAATATCAGCAGCAATATGTGGGTTTCTCCCAAATGCATCATCATCCTTCTCAGTCCATTGCTGTACCGTCCTCTGCAACCGCTAGTTATGCTTATGAATATGCAAATCCTTCACATGAACAAGTGTTCTACACCCAGCATCCACCTGCCCCATTGCCTCCTCAATATCAAAGTATGACTCCAGCAGCGGCAGCAGCAGCTGTAGCATTATCAGATGATTCGAAACAGCAGCTACCTACATACTCAGCAGCAGATCAAAACCACACAACCATAAGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

706

Amino Acids

76.72

Weight (kDa)

5.15

Isoelectric Point (pI)

70.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1788
AarI CACCTGC 1 cut(s) 1990
Acc36I ACCTGC 1 cut(s) 1990
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 2 cut(s) 120, 791
AccII CGCG 1 cut(s) 454
AclWI GGATC 3 cut(s) 12, 1065, 1237
AcoI YGGCCR 4 cut(s) 187, 316, 594, 1609
AcsI RAATTY 1 cut(s) 628
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 8 cut(s) 252, 338, 723, 916, 979, 1229, 1355, 1902
AfiI CCNNNNNNNGG 4 cut(s) 537, 974, 1007, 1499
AgsI TTSAA 2 cut(s) 445, 1334
AjnI CCWGG 1 cut(s) 151
AjuI GAANNNNNNNTTGG 2 cut(s) 1314, 1346
AleI CACNNNNGTG 1 cut(s) 1664
AluBI AGCT 8 cut(s) 163, 180, 267, 343, 1558, 1570, 2036, 2068
AluI AGCT 8 cut(s) 163, 180, 267, 343, 1558, 1570, 2036, 2068
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 3 cut(s) 12, 1065, 1237
AlwNI CAGNNNCTG 1 cut(s) 2036
AoxI GGCC 6 cut(s) 187, 316, 320, 594, 1609, 1668
ApoI RAATTY 1 cut(s) 628
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AseI ATTAAT 1 cut(s) 690
AspLEI GCGC 3 cut(s) 158, 553, 1510
AspS9I GGNCC 2 cut(s) 303, 1274
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 8 cut(s) 31, 370, 668, 680, 744, 1158, 1556, 1746
AsuII TTCGAA 1 cut(s) 2057
AvaII GGWCC 2 cut(s) 303, 1274
BalI TGGCCA 1 cut(s) 1611
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 360
BccI CCATC 2 cut(s) 946, 979
BceAI ACGGC 1 cut(s) 441
BcgI CGANNNNNNTGC 2 cut(s) 1160, 1194
BciT130I CCWGG 1 cut(s) 153
BciVI GTATCC 1 cut(s) 1596
BclI TGATCA 2 cut(s) 1375, 1573
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 4 cut(s) 855, 1257, 1920, 2116
BfmI CTRYAG 1 cut(s) 2037
BfoI RGCGCY 2 cut(s) 159, 1511
BfuAI ACCTGC 1 cut(s) 1990
BfuI GTATCC 1 cut(s) 1596
BglI GCCNNNNNGGC 1 cut(s) 454
BglII AGATCT 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 2 cut(s) 153, 301
Bme18I GGWCC 2 cut(s) 303, 1274
BmgT120I GGNCC 2 cut(s) 303, 1274
BmiI GGNNCC 5 cut(s) 6, 157, 305, 458, 1318
BmrFI CCNGG 2 cut(s) 153, 301
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 9 cut(s) 511, 538, 852, 1208, 1262, 1291, 1590, 1882, 1984
BmuI ACTGGG 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 2 cut(s) 1029, 2001
Bpu14I TTCGAA 1 cut(s) 2057
BpuEI CTTGAG 3 cut(s) 317, 636, 1657
BpuMI CCSGG 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 6 cut(s) 51, 104, 151, 313, 501, 600
BsaXI ACNNNNNCTCC 9 cut(s) 30, 272, 302, 1002, 1032, 1199, 1229, 1348, 1378
Bsc4I CCNNNNNNNGG 4 cut(s) 537, 974, 1007, 1499
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 5 cut(s) 622, 922, 1070, 1589, 1612
Bse3DI GCAATG 2 cut(s) 1893, 1988
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 6 cut(s) 51, 104, 151, 313, 501, 600
BseGI GGATG 6 cut(s) 136, 195, 847, 1685, 1879, 1975
BseLI CCNNNNNNNGG 4 cut(s) 537, 974, 1007, 1499
BseMI GCAATG 2 cut(s) 1893, 1988
BseMII CTCAG 5 cut(s) 102, 387, 995, 1901, 2094
BseNI ACTGG 5 cut(s) 622, 922, 1070, 1589, 1612
BseRI GAGGAG 5 cut(s) 259, 299, 1080, 1161, 1986
BseYI CCCAGC 1 cut(s) 1971
BsgI GTGCAG 2 cut(s) 196, 918
Bsh1236I CGCG 1 cut(s) 454
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 6 cut(s) 189, 318, 322, 596, 1611, 1670
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 421
BsiSI CCGG 2 cut(s) 301, 319
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 4 cut(s) 537, 974, 1007, 1499
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 2 cut(s) 733, 997
BsnI GGCC 6 cut(s) 189, 318, 322, 596, 1611, 1670
Bso31I GGTCTC 1 cut(s) 114
Bsp119I TTCGAA 1 cut(s) 2057
Bsp19I CCATGG 4 cut(s) 51, 313, 501, 600
BspANI GGCC 6 cut(s) 189, 318, 322, 596, 1611, 1670
BspCNI CTCAG 5 cut(s) 101, 386, 994, 1900, 2093
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 6, 157, 305, 458, 1318
BspMI ACCTGC 1 cut(s) 1990
BspPI GGATC 3 cut(s) 12, 1065, 1237
BspT104I TTCGAA 1 cut(s) 2057
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 1893, 1988
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 5 cut(s) 622, 922, 1070, 1589, 1612
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 6 cut(s) 51, 104, 151, 313, 501, 600
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 5 cut(s) 51, 104, 313, 501, 600
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 900, 1905
Bst6I CTCTTC 2 cut(s) 30, 476
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 1114
BstBI TTCGAA 1 cut(s) 2057
BstC8I GCNNGC 4 cut(s) 68, 206, 320, 1780
BstDEI CTNAG 5 cut(s) 88, 373, 981, 1887, 2080
BstDSI CCRYGG 4 cut(s) 51, 313, 501, 600
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 6 cut(s) 136, 195, 847, 1685, 1879, 1975
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 2 cut(s) 159, 1511
BstHHI GCGC 3 cut(s) 158, 553, 1510
BstMAI GTCTC 1 cut(s) 114
BstMCI CGRYCG 1 cut(s) 421
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 70, 1633
BstSCI CCNGG 2 cut(s) 151, 299
BstSFI CTRYAG 1 cut(s) 2037
BstUI CGCG 1 cut(s) 454
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 3 cut(s) 4, 391, 1242
BstXI CCANNNNNNTGG 2 cut(s) 52, 1666
BstYI RGATCY 3 cut(s) 4, 391, 1242
BsuI GTATCC 1 cut(s) 1596
BsuRI GGCC 6 cut(s) 189, 318, 322, 596, 1611, 1670
BtgI CCRYGG 4 cut(s) 51, 313, 501, 600
BtgZI GCGATG 2 cut(s) 783, 1314
BtsCI GGATG 6 cut(s) 136, 195, 847, 1685, 1879, 1975
BtsI GCAGTG 1 cut(s) 1659
BtsIMutI CAGTG 1 cut(s) 1659
BveI ACCTGC 1 cut(s) 1990
Cac8I GCNNGC 4 cut(s) 68, 206, 320, 1780
CaiI CAGNNNCTG 1 cut(s) 2036
CfoI GCGC 3 cut(s) 158, 553, 1510
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 2 cut(s) 303, 1274
CseI GACGC 2 cut(s) 131, 486
Csp6I GTAC 8 cut(s) 251, 337, 722, 915, 978, 1228, 1354, 1901
CviQI GTAC 8 cut(s) 251, 337, 722, 915, 978, 1228, 1354, 1901
DdeI CTNAG 5 cut(s) 88, 373, 981, 1887, 2080
DinI GGCGCC 1 cut(s) 157
EaeI YGGCCR 4 cut(s) 187, 316, 594, 1609
Eam1104I CTCTTC 2 cut(s) 30, 476
EarI CTCTTC 2 cut(s) 30, 476
Eco130I CCWWGG 5 cut(s) 51, 104, 313, 501, 600
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 303, 1274
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoO109I RGGNCCY 1 cut(s) 1274
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 5 cut(s) 51, 104, 313, 501, 600
EcoT22I ATGCAT 1 cut(s) 1875
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 5 cut(s) 51, 104, 313, 501, 600
FaqI GGGAC 1 cut(s) 85
FauI CCCGC 4 cut(s) 209, 213, 251, 299
FbaI TGATCA 2 cut(s) 1375, 1573
FblI GTMKAC 2 cut(s) 120, 791
FokI GGATG 6 cut(s) 143, 182, 854, 1672, 1866, 1962
FseI GGCCGGCC 1 cut(s) 322
FspBI CTAG 4 cut(s) 855, 1257, 1920, 2116
GlaI GCGC 3 cut(s) 157, 552, 1509
GsaI CCCAGC 1 cut(s) 1975
GsuI CTGGAG 2 cut(s) 1029, 2001
HaeII RGCGCY 2 cut(s) 159, 1511
HaeIII GGCC 6 cut(s) 189, 318, 322, 596, 1611, 1670
HapII CCGG 2 cut(s) 301, 319
HgaI GACGC 2 cut(s) 131, 486
HhaI GCGC 3 cut(s) 158, 553, 1510
Hin1I GRCGYC 1 cut(s) 156
Hin6I GCGC 3 cut(s) 156, 551, 1508
HinP1I GCGC 3 cut(s) 156, 551, 1508
HincII GTYRAC 3 cut(s) 94, 121, 615
HindII GTYRAC 3 cut(s) 94, 121, 615
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 2 cut(s) 301, 319
HphI GGTGA 8 cut(s) 31, 370, 668, 680, 744, 1158, 1556, 1746
Hpy166II GTNNAC 7 cut(s) 94, 121, 615, 792, 1021, 1356, 1711
Hpy188III TCNNGA 5 cut(s) 111, 395, 746, 1008, 1343
Hpy8I GTNNAC 7 cut(s) 94, 121, 615, 792, 1021, 1356, 1711
Hpy99I CGWCG 5 cut(s) 125, 233, 389, 548, 1057
HpyAV CCTTC 6 cut(s) 459, 831, 1173, 1839, 1893, 1956
HpyCH4III ACNGT 2 cut(s) 900, 1905
HpyCH4IV ACGT 1 cut(s) 228
HpyF3I CTNAG 5 cut(s) 88, 373, 981, 1887, 2080
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 156
HspAI GCGC 3 cut(s) 156, 551, 1508
KasI GGCGCC 1 cut(s) 155
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 2 cut(s) 1375, 1573
LmnI GCTCC 4 cut(s) 272, 293, 462, 1543
LweI GCATC 9 cut(s) 511, 538, 852, 1208, 1262, 1291, 1590, 1882, 1984
MaeI CTAG 4 cut(s) 855, 1257, 1920, 2116
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 8 cut(s) 376, 635, 656, 668, 750, 872, 1614, 2111
MboII GAAGA 6 cut(s) 17, 365, 401, 422, 463, 715
MflI RGATCY 3 cut(s) 4, 391, 1242
MlsI TGGCCA 1 cut(s) 1611
MluCI AATT 4 cut(s) 628, 814, 952, 1457
MluNI TGGCCA 1 cut(s) 1611
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 7 cut(s) 80, 89, 356, 542, 584, 758, 2008
MmeI TCCRAC 2 cut(s) 303, 1035
Mox20I TGGCCA 1 cut(s) 1611
Mph1103I ATGCAT 1 cut(s) 1875
MroNI GCCGGC 1 cut(s) 318
MscI TGGCCA 1 cut(s) 1611
MseI TTAA 5 cut(s) 555, 690, 711, 1122, 1682
MslI CAYNNNNRTG 3 cut(s) 50, 65, 1664
Msp20I TGGCCA 1 cut(s) 1611
MspA1I CMGCKG 3 cut(s) 1706, 2024, 2036
MspI CCGG 2 cut(s) 301, 319
MspR9I CCNGG 2 cut(s) 153, 301
Mva1269I GAATGC 2 cut(s) 733, 997
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 454
NaeI GCCGGC 1 cut(s) 320
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 1 cut(s) 301
NcoI CCATGG 4 cut(s) 51, 313, 501, 600
NgoMIV GCCGGC 1 cut(s) 318
NlaIV GGNNCC 5 cut(s) 6, 157, 305, 458, 1318
NmeAIII GCCGAG 1 cut(s) 572
NmuCI GTSAC 7 cut(s) 376, 656, 668, 750, 872, 1614, 2111
NsiI ATGCAT 1 cut(s) 1875
NspI RCATGY 2 cut(s) 70, 1633
NspV TTCGAA 1 cut(s) 2057
OliI CACNNNNGTG 1 cut(s) 1664
PaeI GCATGC 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 1990
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 393, 414
PctI GAATGC 2 cut(s) 733, 997
PdiI GCCGGC 1 cut(s) 320
PfeI GAWTC 4 cut(s) 826, 1172, 1346, 2054
PfoI TCCNGGA 1 cut(s) 299
PleI GAGTC 7 cut(s) 80, 89, 356, 541, 584, 757, 2008
PluTI GGCGCC 1 cut(s) 159
PpsI GAGTC 7 cut(s) 80, 89, 356, 541, 584, 757, 2008
PpuMI RGGWCCY 1 cut(s) 1274
PshBI ATTAAT 1 cut(s) 690
PsiI TTATAA 1 cut(s) 1788
Psp5II RGGWCCY 1 cut(s) 1274
Psp6I CCWGG 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 1971
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 5 cut(s) 6, 157, 305, 458, 1318
PspPI GGNCC 2 cut(s) 303, 1274
PspPPI RGGWCCY 1 cut(s) 1274
PstNI CAGNNNCTG 1 cut(s) 2036
PsuI RGATCY 3 cut(s) 4, 391, 1242
PvuII CAGCTG 1 cut(s) 2036
RigI GGCCGGCC 1 cut(s) 322
RsaI GTAC 8 cut(s) 252, 338, 723, 916, 979, 1229, 1355, 1902
RsaNI GTAC 8 cut(s) 251, 337, 722, 915, 978, 1228, 1354, 1901
RseI CAYNNNNRTG 3 cut(s) 50, 65, 1664
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 5 cut(s) 555, 690, 711, 1122, 1682
Sau96I GGNCC 2 cut(s) 303, 1274
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 7 cut(s) 80, 89, 356, 542, 584, 758, 2008
ScrFI CCNGG 2 cut(s) 153, 301
SfaNI GCATC 9 cut(s) 511, 538, 852, 1208, 1262, 1291, 1590, 1882, 1984
SfcI CTRYAG 1 cut(s) 2037
SfoI GGCGCC 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 2057
SinI GGWCC 2 cut(s) 303, 1274
SmiMI CAYNNNNRTG 3 cut(s) 50, 65, 1664
SmlI CTYRAG 3 cut(s) 332, 651, 1672
SmoI CTYRAG 3 cut(s) 332, 651, 1672
SphI GCATGC 1 cut(s) 70
Sse9I AATT 4 cut(s) 628, 814, 952, 1457
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 4 cut(s) 855, 1257, 1920, 2116
StyD4I CCNGG 2 cut(s) 151, 299
StyI CCWWGG 5 cut(s) 51, 104, 313, 501, 600
TaaI ACNGT 2 cut(s) 900, 1905
TaiI ACGT 1 cut(s) 231
TaqI TCGA 8 cut(s) 112, 120, 396, 408, 546, 737, 824, 2057
TasI AATT 4 cut(s) 628, 814, 952, 1457
TatI WGTACW 4 cut(s) 250, 721, 914, 1227
TauI GCSGC 6 cut(s) 189, 237, 457, 970, 1680, 2027
TfiI GAWTC 4 cut(s) 826, 1172, 1346, 2054
Tru1I TTAA 5 cut(s) 555, 690, 711, 1122, 1682
Tru9I TTAA 5 cut(s) 555, 690, 711, 1122, 1682
TscAI CASTG 1 cut(s) 1666
TseFI GTSAC 7 cut(s) 376, 656, 668, 750, 872, 1614, 2111
Tsp45I GTSAC 7 cut(s) 376, 656, 668, 750, 872, 1614, 2111
TspDTI ATGAA 6 cut(s) 716, 786, 1004, 1550, 1947, 1968
TspGWI ACGGA 1 cut(s) 1800
TspRI CASTG 1 cut(s) 1666
VpaK11BI GGWCC 2 cut(s) 303, 1274
VspI ATTAAT 1 cut(s) 690
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 1 cut(s) 628
XceI RCATGY 2 cut(s) 70, 1633
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmiI GTMKAC 2 cut(s) 120, 791
XspI CTAG 4 cut(s) 855, 1257, 1920, 2116
ZrmI AGTACT 1 cut(s) 916
Zsp2I ATGCAT 1 cut(s) 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.