Rmu_sc0019996.1_g000002

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0019996.1
OG0011787
Physical Location & Seq
Reverse (-)
8898 .. 11266
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0019996.1_g000002.1.cds

Sequence Viewer

Length: 2118 bp
atggatccaccgcctctcccaccgcttccggccgccacggccgccacgcaactcggctaccccgattcactcgactcctccccccgatcccgcaatgccgactcttcttgggacgccgagcctctccctcaggtccccggcgccaagctccgcctcatgtgcagctacggcggccacatcctcccccgcccgcacgacaagtcgctctgctacgtcggcggcgagactcgcattgtcgtcgccgaacgccactcctccctctccgacctctgctcccgcctctcccgtaccctcctcaacggccgccccttctctctcaagtaccagctcccgacggaggacctcgactccctcatctccgtcaccaccgacgaggatctcgacaacatggtggaggagtacgaccgcacctcgtcctccgcctcgccgttgaagccgtcgtcgcggctccgtctctttctcttcttcggcaaaccggacacggcggcgtccatgggggccttgctcgacgacgccaagtccgagtcgtggttcgtcgacgcgctcaacggctcgggtctgatgccgcgaaacctctccgactcggcggccaccatggactgcttgttgaccggcgggagtgattcttgcaacgacttggaggcggcggcggcgagtcaaggcgacttctttggggatcatgacagtggaaaacctagtgtggtggttcaagaagtgcattcgatttcggaatcggcctttgtggagagcaatacgtcgtcctctgggtcgtcttcttcgtcgccttgcttgtcaaatttgccgccaattagggttagaatggacggaaatgtggccggagtagtgcagggtcagaagccggctgggattgaggagcagtttgctcagataagctttgctgctgcaccaacaatggtggctgctgctggttctggagctttgagtactaatcctatggctgtgaactcaggaggaggtgaaactatgaacaggatcaactcggaggatgatatttctggtcagaatgtgcctgtgggttttcgaaagccgcctttgccaatgccattgcagccgcagcattttaagcagggaggtggaggtggaggttatagcttgccttcaccggattcagttgcaagtgatagtagcattgcgtctgcaaactctctgtcgaaaccaatgtactatcaagagcaagttcaagttgcagctagagatggcagagctcctgttagcccgaatacaacaagtgatgcttcggatcagggttcccgaaaccaagttcaacaagttcaggattcagggtacagtgtgcctccgcaatcagatcaacaacagcaaattcagcttcagcaacaacagcagcaacagcaacaactgctacagcagcagcagcagcaacaacaacaacagctccaacagcagcatctccagcagcagcaacagcagcagcaacaatttgtccatgcaagcgcgcaatatatgaatattcagcaccctgctactggtcaagtgccagtgtcaccgtactacacaatgtatgctccaccatcgcagcagcaacagcttcatcaccaaattgatcatcagcagcagtatccagtctatttgatgcctgttgcacagtcgcaacaaccatacaagatggctctgcagtctacaatagctgatccagctggtgttgcctcaacccgcattacatcccaaaaccctgctgcttacaaggacagtatcccacccatttatcccccaaagacagcttcacctgctatgcctgaaatgactcaaagtgtttaccagacctctgtgacaccaactcctcaattagtccaaatcccttccaaccaatatcagcagcaatttgttggttactctcaaatgcatcatccttctcagtccattgctgttccttcctctgcgacagcacccaactatgcgtacgaatatgccaatccttcacatgaacaagtatactacacccagcatcaagcagccccattgccttctcaatatcaatcgatgactccagcggccgccgccgcagcagcagcaatgtcagatgaatcaaagcaggtagccgcatactcagcagcagatcaaagccacacacaatcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

705

Amino Acids

75.85

Weight (kDa)

5.0

Isoelectric Point (pI)

70.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1774
Acc36I ACCTGC 2 cut(s) 1774, 2062
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 3 cut(s) 537, 1658, 1971
AccII CGCG 4 cut(s) 445, 542, 568, 1475
AclWI GGATC 7 cut(s) 12, 81, 384, 684, 1001, 1269, 1664
AcoI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 834, 2031
AcsI RAATTY 2 cut(s) 796, 1341
AcuI CTGAAG 1 cut(s) 1334
AcyI GRCGYC 4 cut(s) 114, 141, 488, 513
AfaI GTAC 8 cut(s) 289, 323, 401, 946, 1184, 1307, 1529, 1940
AfiI CCNNNNNNNGG 3 cut(s) 337, 528, 1505
AgsI TTSAA 4 cut(s) 433, 710, 1202, 1286
AhdI GACNNNNNGTC 1 cut(s) 517
AjuI GAANNNNNNNTTGG 2 cut(s) 1744, 1776
Alw21I GWGCWC 1 cut(s) 1228
Alw26I GTCTC 2 cut(s) 218, 458
AlwI GGATC 7 cut(s) 12, 81, 384, 684, 1001, 1269, 1664
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 9 cut(s) 30, 39, 172, 301, 498, 588, 735, 834, 2031
ApoI RAATTY 2 cut(s) 796, 1341
ArsI GACNNNNNNTTYG 2 cut(s) 1154, 1186
AspLEI GCGC 4 cut(s) 143, 544, 1475, 1477
AspS9I GGNCC 3 cut(s) 133, 340, 498
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 6 cut(s) 355, 989, 1113, 1515, 1565, 1755
AsuII TTCGAA 1 cut(s) 1042
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 133, 340
AxyI CCTNAGG 1 cut(s) 129
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 1228
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 1228
BccI CCATC 3 cut(s) 1211, 1558, 1639
BceAI ACGGC 7 cut(s) 54, 184, 316, 412, 421, 498, 565
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciVI GTATCC 2 cut(s) 1608, 1742
BclI TGATCA 1 cut(s) 1582
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 218, 458
BfaI CTAG 2 cut(s) 696, 1212
BfmI CTRYAG 2 cut(s) 1382, 1652
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 2 cut(s) 1774, 2062
BfuI GTATCC 2 cut(s) 1608, 1742
BglI GCCNNNNNGGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 2 cut(s) 133, 340
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 133, 340, 498
BmiI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1270
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 6 cut(s) 552, 1243, 1435, 1602, 1891, 1993
BpiI GAAGAC 1 cut(s) 765
BpmI CTGGAG 3 cut(s) 954, 1415, 2010
Bpu14I TTCGAA 1 cut(s) 1042
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 2018
BsaHI GRCGYC 4 cut(s) 114, 141, 488, 513
BsaJI CCNNGG 4 cut(s) 36, 136, 492, 594
BsaWI WCCGGW 2 cut(s) 475, 1123
BsaXI ACNNNNNCTCC 9 cut(s) 30, 257, 287, 332, 362, 1398, 1428, 1801, 1831
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 528, 1505
Bse118I RCCGGY 2 cut(s) 611, 859
Bse1I ACTGG 3 cut(s) 1510, 1517, 1601
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 6 cut(s) 100, 1064, 1149, 1899, 1997, 2058
BseCI ATCGAT 1 cut(s) 2018
BseDI CCNNGG 4 cut(s) 36, 136, 492, 594
BseGI GGATG 4 cut(s) 177, 1012, 1700, 1885
BseLI CCNNNNNNNGG 3 cut(s) 337, 528, 1505
BseMI GCAATG 6 cut(s) 100, 1064, 1149, 1899, 1997, 2058
BseMII CTCAG 5 cut(s) 143, 899, 981, 1907, 2100
BseNI ACTGG 3 cut(s) 1510, 1517, 1601
BsePI GCGCGC 1 cut(s) 1473
BseRI GAGGAG 7 cut(s) 67, 244, 284, 410, 887, 987, 1809
BseX3I CGGCCG 4 cut(s) 30, 39, 301, 2031
BseYI CCCAGC 2 cut(s) 863, 1980
BsgI GTGCAG 3 cut(s) 181, 866, 888
Bsh1236I CGCG 4 cut(s) 445, 542, 568, 1475
Bsh1285I CGRYCG 5 cut(s) 33, 42, 304, 406, 2034
BshFI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 737, 836, 2033
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 2018
BsiEI CGRYCG 5 cut(s) 33, 42, 304, 406, 2034
BsiHKAI GWGCWC 1 cut(s) 1228
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 7 cut(s) 29, 138, 476, 612, 837, 860, 1124
BsiWI CGTACG 1 cut(s) 1938
BslFI GGGAC 2 cut(s) 119, 125
BslI CCNNNNNNNGG 3 cut(s) 337, 528, 1505
BsmAI GTCTC 2 cut(s) 218, 458
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 119, 125
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 737, 836, 2033
BsoBI CYCGRG 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 1042
Bsp1286I GDGCHC 1 cut(s) 1228
Bsp19I CCATGG 2 cut(s) 492, 594
BspANI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 737, 836, 2033
BspCNI CTCAG 5 cut(s) 142, 898, 980, 1906, 2099
BspDI ATCGAT 1 cut(s) 2018
BspFNI CGCG 4 cut(s) 445, 542, 568, 1475
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1270
BspMAI CTGCAG 1 cut(s) 1656
BspMI ACCTGC 2 cut(s) 1774, 2062
BspPI GGATC 7 cut(s) 12, 81, 384, 684, 1001, 1269, 1664
BspT104I TTCGAA 1 cut(s) 1042
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 6 cut(s) 100, 1064, 1149, 1899, 1997, 2058
BsrFI RCCGGY 2 cut(s) 611, 859
BsrI ACTGG 3 cut(s) 1510, 1517, 1601
BssAI RCCGGY 2 cut(s) 611, 859
BssECI CCNNGG 4 cut(s) 36, 136, 492, 594
BssHII GCGCGC 1 cut(s) 1473
BssNAI GTATAC 1 cut(s) 1972
BssNI GRCGYC 4 cut(s) 114, 141, 488, 513
BssT1I CCWWGG 2 cut(s) 492, 594
Bst1107I GTATAC 1 cut(s) 1972
Bst4CI ACNGT 5 cut(s) 686, 1310, 1527, 1626, 1730
Bst6I CTCTTC 2 cut(s) 109, 467
BstACI GRCGYC 4 cut(s) 114, 141, 488, 513
BstAPI GCANNNNNTGC 1 cut(s) 1378
BstBI TTCGAA 1 cut(s) 1042
BstC8I GCNNGC 5 cut(s) 191, 861, 1115, 1471, 1475
BstDEI CTNAG 5 cut(s) 129, 885, 967, 1893, 2086
BstDSI CCRYGG 3 cut(s) 36, 492, 594
BstF5I GGATG 4 cut(s) 177, 1012, 1700, 1885
BstFNI CGCG 4 cut(s) 445, 542, 568, 1475
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 143, 544, 1475, 1477
BstMAI GTCTC 2 cut(s) 218, 458
BstMCI CGRYCG 5 cut(s) 33, 42, 304, 406, 2034
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 2 cut(s) 1382, 1652
BstUI CGCG 4 cut(s) 445, 542, 568, 1475
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 2 cut(s) 4, 376
BstYI RGATCY 2 cut(s) 4, 376
BstZ17I GTATAC 1 cut(s) 1972
BstZI CGGCCG 4 cut(s) 30, 39, 301, 2031
Bsu15I ATCGAT 1 cut(s) 2018
Bsu36I CCTNAGG 1 cut(s) 129
BsuI GTATCC 2 cut(s) 1608, 1742
BsuRI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 737, 836, 2033
BsuTUI ATCGAT 1 cut(s) 2018
BtgI CCRYGG 3 cut(s) 36, 492, 594
BtgZI GCGATG 1 cut(s) 1536
BtsCI GGATG 4 cut(s) 177, 1012, 1700, 1885
BtsIMutI CAGTG 3 cut(s) 691, 1315, 1524
BveI ACCTGC 2 cut(s) 1774, 2062
Cac8I GCNNGC 5 cut(s) 191, 861, 1115, 1471, 1475
CciI TCATGA 1 cut(s) 679
CciNI GCGGCCGC 1 cut(s) 2031
CfoI GCGC 4 cut(s) 143, 544, 1475, 1477
Cfr10I RCCGGY 2 cut(s) 611, 859
Cfr13I GGNCC 3 cut(s) 133, 340, 498
ClaI ATCGAT 1 cut(s) 2018
CseI GACGC 5 cut(s) 122, 477, 521, 548, 1143
Csp6I GTAC 8 cut(s) 288, 322, 400, 945, 1183, 1306, 1528, 1939
CspCI CAANNNNNGTGG 2 cut(s) 897, 932
CviAII CATG 7 cut(s) 157, 388, 493, 595, 680, 1466, 1961
CviQI GTAC 8 cut(s) 288, 322, 400, 945, 1183, 1306, 1528, 1939
DdeI CTNAG 5 cut(s) 129, 885, 967, 1893, 2086
DinI GGCGCC 1 cut(s) 142
DriI GACNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 834, 2031
EagI CGGCCG 4 cut(s) 30, 39, 301, 2031
Eam1104I CTCTTC 2 cut(s) 109, 467
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 2 cut(s) 109, 467
EciI GGCGGA 2 cut(s) 140, 409
Ecl136II GAGCTC 1 cut(s) 1226
EclXI CGGCCG 4 cut(s) 30, 39, 301, 2031
Eco130I CCWWGG 2 cut(s) 492, 594
Eco24I GRGCYC 1 cut(s) 1228
Eco47I GGWCC 2 cut(s) 133, 340
Eco52I CGGCCG 4 cut(s) 30, 39, 301, 2031
Eco53kI GAGCTC 1 cut(s) 1226
Eco57I CTGAAG 1 cut(s) 1334
Eco81I CCTNAGG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 1226
EcoO109I RGGNCCY 3 cut(s) 133, 340, 498
EcoT14I CCWWGG 2 cut(s) 492, 594
EcoT22I ATGCAT 1 cut(s) 1884
EcoT38I GRGCYC 1 cut(s) 1228
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 492, 594
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 7 cut(s) 160, 391, 496, 598, 683, 1469, 1964
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 2 cut(s) 119, 125
FatI CATG 7 cut(s) 156, 387, 492, 594, 679, 1465, 1960
FauI CCCGC 6 cut(s) 98, 194, 198, 284, 608, 1700
FbaI TGATCA 1 cut(s) 1582
FblI GTMKAC 3 cut(s) 537, 1658, 1971
FokI GGATG 4 cut(s) 164, 1019, 1687, 1872
FriOI GRGCYC 1 cut(s) 1228
FspBI CTAG 2 cut(s) 696, 1212
GlaI GCGC 4 cut(s) 142, 543, 1474, 1476
GsaI CCCAGC 2 cut(s) 867, 1984
GsuI CTGGAG 3 cut(s) 954, 1415, 2010
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 737, 836, 2033
HapII CCGG 7 cut(s) 29, 138, 476, 612, 837, 860, 1124
HgaI GACGC 5 cut(s) 122, 477, 521, 548, 1143
HhaI GCGC 4 cut(s) 143, 544, 1475, 1477
Hin1I GRCGYC 4 cut(s) 114, 141, 488, 513
Hin1II CATG 7 cut(s) 160, 391, 496, 598, 683, 1469, 1964
Hin6I GCGC 4 cut(s) 141, 542, 1473, 1475
HinP1I GCGC 4 cut(s) 141, 542, 1473, 1475
HincII GTYRAC 2 cut(s) 538, 609
HindII GTYRAC 2 cut(s) 538, 609
HindIII AAGCTT 1 cut(s) 892
HpaII CCGG 7 cut(s) 29, 138, 476, 612, 837, 860, 1124
HphI GGTGA 6 cut(s) 355, 989, 1113, 1515, 1565, 1755
Hpy166II GTNNAC 6 cut(s) 538, 609, 964, 1659, 1795, 1972
Hpy188III TCNNGA 9 cut(s) 331, 380, 680, 710, 933, 969, 1190, 1272, 1295
Hpy8I GTNNAC 6 cut(s) 538, 609, 964, 1659, 1795, 1972
HpyAV CCTTC 7 cut(s) 319, 1128, 1848, 1899, 1920, 1965, 2013
HpyCH4III ACNGT 5 cut(s) 686, 1310, 1527, 1626, 1730
HpyCH4IV ACGT 2 cut(s) 213, 755
HpyF3I CTNAG 5 cut(s) 129, 885, 967, 1893, 2086
HpySE526I ACGT 2 cut(s) 213, 755
Hsp92I GRCGYC 4 cut(s) 114, 141, 488, 513
Hsp92II CATG 7 cut(s) 160, 391, 496, 598, 683, 1469, 1964
HspAI GCGC 4 cut(s) 141, 542, 1473, 1475
KasI GGCGCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 859
KroNI GCCGGC 1 cut(s) 861
Ksp22I TGATCA 1 cut(s) 1582
LmnI GCTCC 9 cut(s) 153, 278, 333, 453, 874, 935, 1231, 1419, 1549
LweI GCATC 6 cut(s) 552, 1243, 1435, 1602, 1891, 1993
MaeI CTAG 2 cut(s) 696, 1212
MaeII ACGT 2 cut(s) 213, 755
MaeIII GTNAC 4 cut(s) 361, 1521, 1807, 1868
MboII GAAGA 5 cut(s) 96, 454, 457, 765, 768
MflI RGATCY 2 cut(s) 4, 376
MhlI GDGCHC 1 cut(s) 1228
MluCI AATT 7 cut(s) 796, 807, 1341, 1457, 1578, 1823, 1859
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 9 cut(s) 68, 95, 220, 341, 533, 575, 664, 1777, 2017
MmeI TCCRAC 4 cut(s) 288, 603, 1441, 1866
Mph1103I ATGCAT 1 cut(s) 1884
MroNI GCCGGC 1 cut(s) 859
MseI TTAA 1 cut(s) 1083
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 1676, 2030
MspI CCGG 7 cut(s) 29, 138, 476, 612, 837, 860, 1124
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 718
MvnI CGCG 4 cut(s) 445, 542, 568, 1475
NaeI GCCGGC 1 cut(s) 861
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 492, 594
NgoMIV GCCGGC 1 cut(s) 859
NlaIII CATG 7 cut(s) 160, 391, 496, 598, 683, 1469, 1964
NlaIV GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1270
NmeAIII GCCGAG 3 cut(s) 33, 142, 563
NmuCI GTSAC 3 cut(s) 361, 1521, 1807
NotI GCGGCCGC 1 cut(s) 2031
NsiI ATGCAT 1 cut(s) 1884
NspV TTCGAA 1 cut(s) 1042
PagI TCATGA 1 cut(s) 679
PaqCI CACCTGC 1 cut(s) 1774
PauI GCGCGC 1 cut(s) 1473
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 219, 378, 519, 776
PctI GAATGC 1 cut(s) 718
PdiI GCCGGC 1 cut(s) 861
PfeI GAWTC 6 cut(s) 65, 623, 731, 1127, 1298, 2063
Pfl23II CGTACG 1 cut(s) 1938
PleI GAGTC 9 cut(s) 68, 95, 220, 341, 532, 575, 663, 1777, 2017
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 9 cut(s) 68, 95, 220, 341, 532, 575, 663, 1777, 2017
PpuMI RGGWCCY 2 cut(s) 133, 340
Psp124BI GAGCTC 1 cut(s) 1228
Psp5II RGGWCCY 2 cut(s) 133, 340
PspFI CCCAGC 2 cut(s) 863, 1980
PspLI CGTACG 1 cut(s) 1938
PspN4I GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1270
PspPI GGNCC 3 cut(s) 133, 340, 498
PspPPI RGGWCCY 2 cut(s) 133, 340
PstI CTGCAG 1 cut(s) 1656
PsuI RGATCY 2 cut(s) 4, 376
PteI GCGCGC 1 cut(s) 1473
PvuII CAGCTG 1 cut(s) 1676
RsaI GTAC 8 cut(s) 289, 323, 401, 946, 1184, 1307, 1529, 1940
RsaNI GTAC 8 cut(s) 288, 322, 400, 945, 1183, 1306, 1528, 1939
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 1228
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 1083
Sau96I GGNCC 3 cut(s) 133, 340, 498
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 9 cut(s) 68, 95, 220, 341, 533, 575, 664, 1777, 2017
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 1228
SfaNI GCATC 6 cut(s) 552, 1243, 1435, 1602, 1891, 1993
SfcI CTRYAG 2 cut(s) 1382, 1652
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 1042
SgrDI CGTCGACG 1 cut(s) 536
SinI GGWCC 2 cut(s) 133, 340
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 7 cut(s) 796, 807, 1341, 1457, 1578, 1823, 1859
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 1489
SspMI CTAG 2 cut(s) 696, 1212
SstI GAGCTC 1 cut(s) 1228
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 492, 594
TaaI ACNGT 5 cut(s) 686, 1310, 1527, 1626, 1730
TaiI ACGT 2 cut(s) 216, 758
TaqI TCGA 9 cut(s) 72, 345, 381, 507, 537, 722, 1042, 1172, 2018
TasI AATT 7 cut(s) 796, 807, 1341, 1457, 1578, 1823, 1859
TatI WGTACW 2 cut(s) 944, 1182
TfiI GAWTC 6 cut(s) 65, 623, 731, 1127, 1298, 2063
Tru1I TTAA 1 cut(s) 1083
Tru9I TTAA 1 cut(s) 1083
TscAI CASTG 3 cut(s) 691, 1315, 1524
TseFI GTSAC 3 cut(s) 361, 1521, 1807
Tsp45I GTSAC 3 cut(s) 361, 1521, 1807
TspDTI ATGAA 5 cut(s) 1001, 1499, 1559, 1977, 2076
TspGWI ACGGA 4 cut(s) 349, 350, 440, 840
TspRI CASTG 3 cut(s) 691, 1315, 1524
VpaK11BI GGWCC 2 cut(s) 133, 340
XapI RAATTY 2 cut(s) 796, 1341
XmiI GTMKAC 3 cut(s) 537, 1658, 1971
XspI CTAG 2 cut(s) 696, 1212
ZrmI AGTACT 1 cut(s) 946
Zsp2I ATGCAT 1 cut(s) 1884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.