RLG00000005362

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011787
Physical Location & Seq
Reverse (-)
67070041 .. 67073076
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005362

Sequence Viewer

Length: 2124 bp
ATGGATCCACCGCCTCTCCCACCACTTCCGGCCACCGCCGCTGCCACCACGGCCGCCACGCAACTCGGCTACCCCGATTCATTCGACTCCTCCCCCCGATCCCGCAATGCCGACTCTTCTTGGGACGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTCGTCGCTGACCGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAACGGCCGTCCTTTCTCTCTCAAGTACCAGCTCCCGACGGAGGACCTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTCGACAACATGGTGGAGGAGTACGAACGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTCCGTCTCTTTCTCTTCTTCGGCAAACCCGAGACGGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCCGACTCGGCGGCCACCATGGACTGCTTGTTGACAGGCGGGAGTGATTCTTGCAACGACCTGGAGGCGGCGGCGGCGGCGGCGGTGAGTCAAGGCGAAGGCGACTTCTTTGGGGATCATAACAGTGGAAAACCTAGTGTGGTGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCGTTTGTGGAGAGCAATACGTCGTCGTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCGAATTTGCCGCCAATTAGGGTTAGAATGGACGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCTGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTGCTGCTGCACCAACAACGGTGGCTGCTGGTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCTGGTCAGAATGTGCCTGTGGGTTTTCGAAAGCCGCCTTTGCCAATGCCATTGCAGCCGCAGCATTTTAAGCAGGGAGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCGGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCTCTGTCGAAACCAGTGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTCCTGTTAGCCCGAATACAACAAGCGATGCTTCGGATCAGGGTTCCCGAAACCAAGTTCAACAAGTTCAGGATTCAGGGTACAGTGTGCCTCCGCAATCAGATCAACAACAGCAAATTCAGCTTCAGCAACAACAGCAGCAACAGCAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAGCAGCAGCAACAATTTGTCCATGCAAGCGCACAATATATGAATATTCAGCACCCTGGTACCGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAGTATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGGTGGCTCTGCAGTCTACAGTAGCTGATCCAACTGGTGTTGCCTCGACCCGCATTACTTCCCAAAACCCTGCTGCTTACAAGGACAGTATCCCACCCATTTATCCCTCAAAGACGGCTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTATGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCAATATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCGTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCCATGACTCCAGCAGCCGCCGCCGCAGCAGTAGCAATGTCAGATGAATCAAAGCAGAAAAACCAGATGCGGATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

708

Amino Acids

76.08

Weight (kDa)

5.12

Isoelectric Point (pI)

67.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 69 - 159 5.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1801
AasI GACNNNNNNGTC 1 cut(s) 499
Acc36I ACCTGC 1 cut(s) 1801
Acc65I GGTACC 1 cut(s) 1529
AccB1I GGYRCC 2 cut(s) 152, 1529
AccI GTMKAC 3 cut(s) 549, 1685, 1998
AccII CGCG 3 cut(s) 457, 554, 580
AclWI GGATC 8 cut(s) 12, 93, 396, 711, 1028, 1296, 1691, 2123
AcoI YGGCCR 6 cut(s) 30, 51, 184, 313, 600, 861
AcsI RAATTY 2 cut(s) 823, 1368
AcuI CTGAAG 1 cut(s) 1361
AcyI GRCGYC 4 cut(s) 126, 153, 500, 525
AfaI GTAC 9 cut(s) 301, 335, 413, 973, 1211, 1334, 1531, 1556, 1967
AfiI CCNNNNNNNGG 4 cut(s) 349, 540, 655, 1532
AgeI ACCGGT 1 cut(s) 1532
AgsI TTSAA 4 cut(s) 445, 737, 1229, 1313
AhdI GACNNNNNGTC 1 cut(s) 529
AjnI CCWGG 2 cut(s) 648, 1525
Alw21I GWGCWC 1 cut(s) 1255
Alw26I GTCTC 3 cut(s) 230, 470, 485
AlwI GGATC 8 cut(s) 12, 93, 396, 711, 1028, 1296, 1691, 2123
AlwNI CAGNNNCTG 1 cut(s) 1694
Ama87I CYCGRG 2 cut(s) 488, 565
AoxI GGCC 7 cut(s) 30, 51, 184, 313, 510, 600, 861
ApoI RAATTY 2 cut(s) 823, 1368
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
AsiGI ACCGGT 1 cut(s) 1532
Asp718I GGTACC 1 cut(s) 1529
AspLEI GCGC 3 cut(s) 155, 556, 1502
AspS9I GGNCC 3 cut(s) 145, 352, 510
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 7 cut(s) 367, 685, 1016, 1140, 1542, 1592, 1782
AsuII TTCGAA 1 cut(s) 1069
AvaI CYCGRG 2 cut(s) 488, 565
AvaII GGWCC 2 cut(s) 145, 352
AxyI CCTNAGG 1 cut(s) 141
BaeI ACNNNNGTAYC 2 cut(s) 1513, 1546
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 152, 1529
BanII GRGCYC 1 cut(s) 1255
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 1255
BccI CCATC 2 cut(s) 1238, 1585
BceAI ACGGC 9 cut(s) 66, 196, 300, 328, 424, 433, 510, 577, 1800
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 2 cut(s) 650, 1527
BciVI GTATCC 2 cut(s) 1635, 1769
BclI TGATCA 1 cut(s) 1609
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 3 cut(s) 230, 470, 485
BfaI CTAG 2 cut(s) 723, 1239
BfmI CTRYAG 3 cut(s) 1409, 1679, 1686
BfoI RGCGCY 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 1801
BfuI GTATCC 2 cut(s) 1635, 1769
BglI GCCNNNNNGGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 3 cut(s) 150, 650, 1527
Bme18I GGWCC 2 cut(s) 145, 352
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 2 cut(s) 488, 565
BmgT120I GGNCC 3 cut(s) 145, 352, 510
BmiI GGNNCC 7 cut(s) 6, 147, 154, 461, 511, 1297, 1531
BmrFI CCNGG 3 cut(s) 150, 650, 1527
BmsI GCATC 7 cut(s) 564, 1270, 1462, 1629, 1918, 2020, 2100
BpiI GAAGAC 1 cut(s) 792
BpmI CTGGAG 4 cut(s) 671, 981, 1442, 2037
Bpu14I TTCGAA 1 cut(s) 1069
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 2114
BsaHI GRCGYC 4 cut(s) 126, 153, 500, 525
BsaJI CCNNGG 5 cut(s) 48, 148, 504, 606, 1525
BsaWI WCCGGW 2 cut(s) 1150, 1532
Bsc4I CCNNNNNNNGG 4 cut(s) 349, 540, 655, 1532
Bse118I RCCGGY 2 cut(s) 886, 1532
Bse1I ACTGG 4 cut(s) 1205, 1544, 1628, 1708
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 6 cut(s) 112, 1091, 1176, 1926, 2024, 2085
Bse8I GATNNNNATC 1 cut(s) 2114
BseBI CCWGG 2 cut(s) 650, 1527
BseDI CCNNGG 5 cut(s) 48, 148, 504, 606, 1525
BseGI GGATG 3 cut(s) 189, 1039, 1912
BseJI GATNNNNATC 1 cut(s) 2114
BseLI CCNNNNNNNGG 4 cut(s) 349, 540, 655, 1532
BseMI GCAATG 6 cut(s) 112, 1091, 1176, 1926, 2024, 2085
BseMII CTCAG 4 cut(s) 155, 926, 1008, 1934
BseNI ACTGG 4 cut(s) 1205, 1544, 1628, 1708
BseRI GAGGAG 9 cut(s) 79, 256, 296, 415, 418, 422, 914, 1014, 1836
BseX3I CGGCCG 2 cut(s) 51, 313
BseYI CCCAGC 2 cut(s) 890, 2007
BsgI GTGCAG 3 cut(s) 193, 893, 915
Bsh1236I CGCG 3 cut(s) 457, 554, 580
Bsh1285I CGRYCG 2 cut(s) 54, 316
BshFI GGCC 7 cut(s) 32, 53, 186, 315, 512, 602, 863
BshNI GGYRCC 2 cut(s) 152, 1529
BshTI ACCGGT 1 cut(s) 1532
BsiEI CGRYCG 2 cut(s) 54, 316
BsiHKAI GWGCWC 1 cut(s) 1255
BsiHKCI CYCGRG 2 cut(s) 488, 565
BsiSI CCGG 6 cut(s) 29, 150, 864, 887, 1151, 1533
BsiWI CGTACG 1 cut(s) 1965
BslFI GGGAC 2 cut(s) 131, 137
BslI CCNNNNNNNGG 4 cut(s) 349, 540, 655, 1532
BsmAI GTCTC 3 cut(s) 230, 470, 485
BsmBI CGTCTC 2 cut(s) 470, 485
BsmFI GGGAC 2 cut(s) 131, 137
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 7 cut(s) 32, 53, 186, 315, 512, 602, 863
BsoBI CYCGRG 2 cut(s) 488, 565
Bsp119I TTCGAA 1 cut(s) 1069
Bsp1286I GDGCHC 1 cut(s) 1255
Bsp19I CCATGG 2 cut(s) 504, 606
BspANI GGCC 7 cut(s) 32, 53, 186, 315, 512, 602, 863
BspCNI CTCAG 4 cut(s) 154, 925, 1007, 1933
BspFNI CGCG 3 cut(s) 457, 554, 580
BspLI GGNNCC 7 cut(s) 6, 147, 154, 461, 511, 1297, 1531
BspMAI CTGCAG 1 cut(s) 1683
BspMI ACCTGC 1 cut(s) 1801
BspPI GGATC 8 cut(s) 12, 93, 396, 711, 1028, 1296, 1691, 2123
BspT104I TTCGAA 1 cut(s) 1069
BspT107I GGYRCC 2 cut(s) 152, 1529
BsrDI GCAATG 6 cut(s) 112, 1091, 1176, 1926, 2024, 2085
BsrFI RCCGGY 2 cut(s) 886, 1532
BsrI ACTGG 4 cut(s) 1205, 1544, 1628, 1708
BssAI RCCGGY 2 cut(s) 886, 1532
BssECI CCNNGG 5 cut(s) 48, 148, 504, 606, 1525
BssNAI GTATAC 1 cut(s) 1999
BssNI GRCGYC 4 cut(s) 126, 153, 500, 525
BssT1I CCWWGG 2 cut(s) 504, 606
Bst1107I GTATAC 1 cut(s) 1999
Bst2UI CCWGG 2 cut(s) 650, 1527
Bst4CI ACNGT 7 cut(s) 713, 943, 1337, 1554, 1653, 1690, 1757
Bst6I CTCTTC 2 cut(s) 121, 479
BstACI GRCGYC 4 cut(s) 126, 153, 500, 525
BstAPI GCANNNNNTGC 1 cut(s) 1405
BstBI TTCGAA 1 cut(s) 1069
BstC8I GCNNGC 4 cut(s) 203, 888, 1142, 1498
BstDEI CTNAG 4 cut(s) 141, 912, 994, 1920
BstDSI CCRYGG 3 cut(s) 48, 504, 606
BstF5I GGATG 3 cut(s) 189, 1039, 1912
BstFNI CGCG 3 cut(s) 457, 554, 580
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 3 cut(s) 155, 556, 1502
BstMAI GTCTC 3 cut(s) 230, 470, 485
BstMCI CGRYCG 2 cut(s) 54, 316
BstNI CCWGG 2 cut(s) 650, 1527
BstSCI CCNGG 3 cut(s) 148, 648, 1525
BstSFI CTRYAG 3 cut(s) 1409, 1679, 1686
BstUI CGCG 3 cut(s) 457, 554, 580
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 2 cut(s) 4, 388
BstYI RGATCY 2 cut(s) 4, 388
BstZ17I GTATAC 1 cut(s) 1999
BstZI CGGCCG 2 cut(s) 51, 313
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 2 cut(s) 1635, 1769
BsuRI GGCC 7 cut(s) 32, 53, 186, 315, 512, 602, 863
BtgI CCRYGG 3 cut(s) 48, 504, 606
BtgZI GCGATG 2 cut(s) 1293, 1563
BtsCI GGATG 3 cut(s) 189, 1039, 1912
BtsIMutI CAGTG 4 cut(s) 718, 1212, 1342, 1551
BveI ACCTGC 1 cut(s) 1801
Cac8I GCNNGC 4 cut(s) 203, 888, 1142, 1498
CaiI CAGNNNCTG 1 cut(s) 1694
CfoI GCGC 3 cut(s) 155, 556, 1502
Cfr10I RCCGGY 2 cut(s) 886, 1532
Cfr13I GGNCC 3 cut(s) 145, 352, 510
CseI GACGC 5 cut(s) 134, 489, 533, 560, 1170
Csp6I GTAC 9 cut(s) 300, 334, 412, 972, 1210, 1333, 1530, 1555, 1966
CspAI ACCGGT 1 cut(s) 1532
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 7 cut(s) 169, 400, 505, 607, 1493, 1988, 2047
CviQI GTAC 9 cut(s) 300, 334, 412, 972, 1210, 1333, 1530, 1555, 1966
DdeI CTNAG 4 cut(s) 141, 912, 994, 1920
DinI GGCGCC 1 cut(s) 154
DrdI GACNNNNNNGTC 1 cut(s) 499
DriI GACNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 6 cut(s) 30, 51, 184, 313, 600, 861
EagI CGGCCG 2 cut(s) 51, 313
Eam1104I CTCTTC 2 cut(s) 121, 479
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 2 cut(s) 121, 479
EciI GGCGGA 2 cut(s) 152, 421
Ecl136II GAGCTC 1 cut(s) 1253
EclXI CGGCCG 2 cut(s) 51, 313
Eco130I CCWWGG 2 cut(s) 504, 606
Eco24I GRGCYC 1 cut(s) 1255
Eco47I GGWCC 2 cut(s) 145, 352
Eco52I CGGCCG 2 cut(s) 51, 313
Eco53kI GAGCTC 1 cut(s) 1253
Eco57I CTGAAG 1 cut(s) 1361
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 2 cut(s) 488, 565
EcoICRI GAGCTC 1 cut(s) 1253
EcoO109I RGGNCCY 3 cut(s) 145, 352, 510
EcoRII CCWGG 2 cut(s) 648, 1525
EcoT14I CCWWGG 2 cut(s) 504, 606
EcoT22I ATGCAT 1 cut(s) 1911
EcoT38I GRGCYC 1 cut(s) 1255
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 504, 606
Esp3I CGTCTC 2 cut(s) 470, 485
FaeI CATG 7 cut(s) 172, 403, 508, 610, 1496, 1991, 2050
FalI AAGNNNNNCTT 2 cut(s) 1267, 1299
FaqI GGGAC 2 cut(s) 131, 137
FatI CATG 7 cut(s) 168, 399, 504, 606, 1492, 1987, 2046
FauI CCCGC 6 cut(s) 110, 206, 210, 296, 620, 1727
FbaI TGATCA 1 cut(s) 1609
FblI GTMKAC 3 cut(s) 549, 1685, 1998
FokI GGATG 3 cut(s) 176, 1046, 1899
FriOI GRGCYC 1 cut(s) 1255
FspBI CTAG 2 cut(s) 723, 1239
GlaI GCGC 3 cut(s) 154, 555, 1501
GsaI CCCAGC 2 cut(s) 894, 2011
GsuI CTGGAG 4 cut(s) 671, 981, 1442, 2037
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 7 cut(s) 32, 53, 186, 315, 512, 602, 863
HapII CCGG 6 cut(s) 29, 150, 864, 887, 1151, 1533
HgaI GACGC 5 cut(s) 134, 489, 533, 560, 1170
HhaI GCGC 3 cut(s) 155, 556, 1502
Hin1I GRCGYC 4 cut(s) 126, 153, 500, 525
Hin1II CATG 7 cut(s) 172, 403, 508, 610, 1496, 1991, 2050
Hin6I GCGC 3 cut(s) 153, 554, 1500
HinP1I GCGC 3 cut(s) 153, 554, 1500
HincII GTYRAC 2 cut(s) 550, 621
HindII GTYRAC 2 cut(s) 550, 621
HindIII AAGCTT 1 cut(s) 919
HpaII CCGG 6 cut(s) 29, 150, 864, 887, 1151, 1533
HphI GGTGA 7 cut(s) 367, 685, 1016, 1140, 1542, 1592, 1782
Hpy166II GTNNAC 7 cut(s) 550, 621, 991, 1210, 1686, 1822, 1999
Hpy188III TCNNGA 8 cut(s) 343, 392, 737, 960, 996, 1217, 1299, 1322
Hpy8I GTNNAC 7 cut(s) 550, 621, 991, 1210, 1686, 1822, 1999
HpyAV CCTTC 7 cut(s) 680, 1155, 1875, 1926, 1947, 1992, 2040
HpyCH4III ACNGT 7 cut(s) 713, 943, 1337, 1554, 1653, 1690, 1757
HpyCH4IV ACGT 2 cut(s) 225, 782
HpyF3I CTNAG 4 cut(s) 141, 912, 994, 1920
HpySE526I ACGT 2 cut(s) 225, 782
Hsp92I GRCGYC 4 cut(s) 126, 153, 500, 525
Hsp92II CATG 7 cut(s) 172, 403, 508, 610, 1496, 1991, 2050
HspAI GCGC 3 cut(s) 153, 554, 1500
KasI GGCGCC 1 cut(s) 152
KpnI GGTACC 1 cut(s) 1533
KroI GCCGGC 1 cut(s) 886
KroNI GCCGGC 1 cut(s) 888
Ksp22I TGATCA 1 cut(s) 1609
LmnI GCTCC 9 cut(s) 165, 290, 345, 465, 901, 962, 1258, 1446, 1576
LweI GCATC 7 cut(s) 564, 1270, 1462, 1629, 1918, 2020, 2100
MaeI CTAG 2 cut(s) 723, 1239
MaeII ACGT 2 cut(s) 225, 782
MaeIII GTNAC 3 cut(s) 373, 1548, 1895
MboII GAAGA 5 cut(s) 108, 466, 469, 792, 795
MflI RGATCY 2 cut(s) 4, 388
MhlI GDGCHC 1 cut(s) 1255
MluCI AATT 8 cut(s) 823, 834, 1368, 1484, 1605, 1850, 1886, 2119
Mly113I GGCGCC 1 cut(s) 153
MlyI GAGTC 9 cut(s) 80, 107, 232, 353, 545, 587, 685, 1804, 2044
MmeI TCCRAC 5 cut(s) 300, 615, 1468, 1724, 1893
Mph1103I ATGCAT 1 cut(s) 1911
MroNI GCCGGC 1 cut(s) 886
MseI TTAA 1 cut(s) 1110
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 6 cut(s) 29, 150, 864, 887, 1151, 1533
MspR9I CCNGG 3 cut(s) 150, 650, 1527
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 2 cut(s) 650, 1527
MvnI CGCG 3 cut(s) 457, 554, 580
NaeI GCCGGC 1 cut(s) 888
NarI GGCGCC 1 cut(s) 153
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 2 cut(s) 504, 606
NgoMIV GCCGGC 1 cut(s) 886
NlaIII CATG 7 cut(s) 172, 403, 508, 610, 1496, 1991, 2050
NlaIV GGNNCC 7 cut(s) 6, 147, 154, 461, 511, 1297, 1531
NmeAIII GCCGAG 3 cut(s) 45, 154, 575
NmuCI GTSAC 2 cut(s) 373, 1548
NsiI ATGCAT 1 cut(s) 1911
NspV TTCGAA 1 cut(s) 1069
PaqCI CACCTGC 1 cut(s) 1801
PcsI WCGNNNNNNNCGW 8 cut(s) 72, 231, 390, 486, 531, 761, 794, 803
PctI GAATGC 1 cut(s) 745
PdiI GCCGGC 1 cut(s) 888
PfeI GAWTC 6 cut(s) 77, 635, 758, 1154, 1325, 2090
Pfl23II CGTACG 1 cut(s) 1965
PinAI ACCGGT 1 cut(s) 1532
PleI GAGTC 9 cut(s) 80, 107, 232, 353, 544, 587, 684, 1804, 2044
PluTI GGCGCC 1 cut(s) 156
PpsI GAGTC 9 cut(s) 80, 107, 232, 353, 544, 587, 684, 1804, 2044
PpuMI RGGWCCY 2 cut(s) 145, 352
Psp124BI GAGCTC 1 cut(s) 1255
Psp5II RGGWCCY 2 cut(s) 145, 352
Psp6I CCWGG 2 cut(s) 648, 1525
PspFI CCCAGC 2 cut(s) 890, 2007
PspGI CCWGG 2 cut(s) 648, 1525
PspLI CGTACG 1 cut(s) 1965
PspN4I GGNNCC 7 cut(s) 6, 147, 154, 461, 511, 1297, 1531
PspPI GGNCC 3 cut(s) 145, 352, 510
PspPPI RGGWCCY 2 cut(s) 145, 352
PstI CTGCAG 1 cut(s) 1683
PstNI CAGNNNCTG 1 cut(s) 1694
PsuI RGATCY 2 cut(s) 4, 388
RsaI GTAC 9 cut(s) 301, 335, 413, 973, 1211, 1334, 1531, 1556, 1967
RsaNI GTAC 9 cut(s) 300, 334, 412, 972, 1210, 1333, 1530, 1555, 1966
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 1 cut(s) 1255
SalI GTCGAC 1 cut(s) 548
SaqAI TTAA 1 cut(s) 1110
Sau96I GGNCC 3 cut(s) 145, 352, 510
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 9 cut(s) 80, 107, 232, 353, 545, 587, 685, 1804, 2044
ScrFI CCNGG 3 cut(s) 150, 650, 1527
SduI GDGCHC 1 cut(s) 1255
SfaNI GCATC 7 cut(s) 564, 1270, 1462, 1629, 1918, 2020, 2100
SfcI CTRYAG 3 cut(s) 1409, 1679, 1686
SfoI GGCGCC 1 cut(s) 154
SfuI TTCGAA 1 cut(s) 1069
SgrDI CGTCGACG 1 cut(s) 548
SinI GGWCC 2 cut(s) 145, 352
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 8 cut(s) 823, 834, 1368, 1484, 1605, 1850, 1886, 2119
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 1 cut(s) 1516
SspMI CTAG 2 cut(s) 723, 1239
SstI GAGCTC 1 cut(s) 1255
StyD4I CCNGG 3 cut(s) 148, 648, 1525
StyI CCWWGG 2 cut(s) 504, 606
TaaI ACNGT 7 cut(s) 713, 943, 1337, 1554, 1653, 1690, 1757
TaiI ACGT 2 cut(s) 228, 785
TasI AATT 8 cut(s) 823, 834, 1368, 1484, 1605, 1850, 1886, 2119
TatI WGTACW 2 cut(s) 971, 1209
TfiI GAWTC 6 cut(s) 77, 635, 758, 1154, 1325, 2090
Tru1I TTAA 1 cut(s) 1110
Tru9I TTAA 1 cut(s) 1110
TscAI CASTG 4 cut(s) 718, 1212, 1342, 1551
TseFI GTSAC 2 cut(s) 373, 1548
Tsp45I GTSAC 2 cut(s) 373, 1548
TspDTI ATGAA 6 cut(s) 69, 1028, 1526, 1586, 2004, 2103
TspGWI ACGGA 4 cut(s) 361, 362, 452, 867
TspRI CASTG 4 cut(s) 718, 1212, 1342, 1551
VpaK11BI GGWCC 2 cut(s) 145, 352
XapI RAATTY 2 cut(s) 823, 1368
XmiI GTMKAC 3 cut(s) 549, 1685, 1998
XspI CTAG 2 cut(s) 723, 1239
ZrmI AGTACT 1 cut(s) 973
Zsp2I ATGCAT 1 cut(s) 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.