Rh7DG023600

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011787
Physical Location & Seq
Forward (+)
1880759 .. 1883737
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG023600.1

Sequence Viewer

Length: 2085 bp
ATGGATCCACCGCCTCTCCCACCACTTCCGACCACCACCGCCGCCACGCAATTCGGCTACCCCGATTCACTCGACTCCTCCCCCCGATCCCGCAATGCCGACTCTTCTTGGGACGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTAGTCTCCGACCGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAATGGCCGCCCCTTCTCTCTCAAGTACCAGCTCCCGACGGAGGACCTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTCGACAACATGGTGGAGGAGTACGACCGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTCCGTCTCTTTCTCTTCTTCGGCAAACCGGAGACGGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCCGACTCGGCGGCCACCATGGACTGCTTGTTGACCGGCGGGAGTGATTCTTGCAACGACTTGGAGGCGGCGGCCAGTCAAGGCGACTTCTTTGGGGATCATGACAGTGGAAAACCTAGTGTGGTGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCCTTTGTGGAGAGCAATACGTCGTCCTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCAAATTTGCCGCCAATTAGGGTTAGAATGGATGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCGGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTGCTGCTGCACCAACAATGGTGGCTGCTGCTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCTGGTCAGAATGTGCCTGTGGGTTTTCGAAAGCCGCCTTTGCCAATGCCATTGCAGCCGCAGCATTTTAAGCAGGGAGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCGGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCTCTGTCGAAACCAATGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTTCTGTTAGCCCGAATACAACAAGTGATGCTTCGGATCAGGGTTCCCGAAACCAAGTTCAACAAGTTCAGGATTCAGGGTACAGTGTGCCTCCACAATCGGATCAACAACAGCAAATTCAGCTTCAGCAACAACAACAGCAACAGCAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAGCAGCAGCAACAATTTGTCCATGCAAGCGCGCAATATATGAATATTCAGCACCCTGCTACTGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAGTATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGATGGCTCTGCAGTCTACAATAGCTGATCCAACTGGTGTTGCCTCGACCCGCATTACTTCCCAAAACCCTGCTGCTTACAAGGACAGTATCCTACCCATTTATCCCTCAAAGACAGCTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTGTGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCAATATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCGTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCCATGACTCCAGCAGCCGCTGCCGCAGCAGTAGCAATGTCAGATGAATCAAAGCAGAAAAACCAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

694

Amino Acids

75.02

Weight (kDa)

5.05

Isoelectric Point (pI)

69.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 65 - 155 1.1e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1771
AasI GACNNNNNNGTC 1 cut(s) 487
Acc36I ACCTGC 1 cut(s) 1771
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 3 cut(s) 537, 1655, 1968
AccII CGCG 4 cut(s) 445, 542, 568, 1472
AclWI GGATC 8 cut(s) 12, 81, 384, 681, 998, 1266, 1332, 1661
AcoI YGGCCR 5 cut(s) 172, 301, 588, 648, 831
AcsI RAATTY 2 cut(s) 793, 1338
AcuI CTGAAG 1 cut(s) 1331
AcyI GRCGYC 4 cut(s) 114, 141, 488, 513
AfaI GTAC 8 cut(s) 289, 323, 401, 943, 1181, 1304, 1526, 1937
AfiI CCNNNNNNNGG 3 cut(s) 337, 528, 1502
AgsI TTSAA 4 cut(s) 433, 707, 1199, 1283
AhdI GACNNNNNGTC 1 cut(s) 517
Alw26I GTCTC 4 cut(s) 218, 244, 458, 473
AlwI GGATC 8 cut(s) 12, 81, 384, 681, 998, 1266, 1332, 1661
AlwNI CAGNNNCTG 1 cut(s) 2033
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 7 cut(s) 172, 301, 498, 588, 648, 732, 831
ApoI RAATTY 2 cut(s) 793, 1338
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
AspLEI GCGC 4 cut(s) 143, 544, 1472, 1474
AspS9I GGNCC 3 cut(s) 133, 340, 498
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 6 cut(s) 355, 986, 1110, 1512, 1562, 1752
AsuII TTCGAA 1 cut(s) 1039
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 133, 340
AxyI CCTNAGG 1 cut(s) 129
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 762
BccI CCATC 4 cut(s) 815, 1208, 1555, 1636
BceAI ACGGC 5 cut(s) 184, 412, 421, 498, 565
BciVI GTATCC 2 cut(s) 1605, 1739
BclI TGATCA 1 cut(s) 1579
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 4 cut(s) 218, 244, 458, 473
BfaI CTAG 2 cut(s) 693, 1209
BfmI CTRYAG 2 cut(s) 1379, 1649
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 1771
BfuI GTATCC 2 cut(s) 1605, 1739
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 2 cut(s) 133, 340
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 133, 340, 498
BmiI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1267
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 6 cut(s) 552, 1240, 1432, 1599, 1888, 1990
BpiI GAAGAC 1 cut(s) 762
BpmI CTGGAG 3 cut(s) 951, 1412, 2007
Bpu14I TTCGAA 1 cut(s) 1039
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 138
BsaHI GRCGYC 4 cut(s) 114, 141, 488, 513
BsaJI CCNNGG 3 cut(s) 136, 492, 594
BsaWI WCCGGW 2 cut(s) 475, 1120
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 528, 1502
Bse118I RCCGGY 2 cut(s) 611, 856
Bse1I ACTGG 5 cut(s) 651, 1507, 1514, 1598, 1678
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 6 cut(s) 100, 1061, 1146, 1896, 1994, 2055
BseDI CCNNGG 3 cut(s) 136, 492, 594
BseGI GGATG 4 cut(s) 177, 826, 1009, 1882
BseLI CCNNNNNNNGG 3 cut(s) 337, 528, 1502
BseMI GCAATG 6 cut(s) 100, 1061, 1146, 1896, 1994, 2055
BseMII CTCAG 4 cut(s) 143, 896, 978, 1904
BseNI ACTGG 5 cut(s) 651, 1507, 1514, 1598, 1678
BsePI GCGCGC 1 cut(s) 1470
BseRI GAGGAG 9 cut(s) 67, 244, 284, 403, 406, 410, 884, 984, 1806
BseYI CCCAGC 1 cut(s) 1977
BsgI GTGCAG 3 cut(s) 181, 863, 885
Bsh1236I CGCG 4 cut(s) 445, 542, 568, 1472
Bsh1285I CGRYCG 2 cut(s) 247, 406
BshFI GGCC 7 cut(s) 174, 303, 500, 590, 650, 734, 833
BshNI GGYRCC 1 cut(s) 140
BsiEI CGRYCG 2 cut(s) 247, 406
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 6 cut(s) 138, 476, 612, 834, 857, 1121
BsiWI CGTACG 1 cut(s) 1935
BslFI GGGAC 2 cut(s) 119, 125
BslI CCNNNNNNNGG 3 cut(s) 337, 528, 1502
BsmAI GTCTC 4 cut(s) 218, 244, 458, 473
BsmBI CGTCTC 2 cut(s) 458, 473
BsmFI GGGAC 2 cut(s) 119, 125
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 7 cut(s) 174, 303, 500, 590, 650, 734, 833
BsoBI CYCGRG 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 1039
Bsp143I GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
Bsp19I CCATGG 2 cut(s) 492, 594
BspANI GGCC 7 cut(s) 174, 303, 500, 590, 650, 734, 833
BspCNI CTCAG 4 cut(s) 142, 895, 977, 1903
BspFNI CGCG 4 cut(s) 445, 542, 568, 1472
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1267
BspMAI CTGCAG 1 cut(s) 1653
BspMI ACCTGC 1 cut(s) 1771
BspPI GGATC 8 cut(s) 12, 81, 384, 681, 998, 1266, 1332, 1661
BspT104I TTCGAA 1 cut(s) 1039
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 6 cut(s) 100, 1061, 1146, 1896, 1994, 2055
BsrFI RCCGGY 2 cut(s) 611, 856
BsrI ACTGG 5 cut(s) 651, 1507, 1514, 1598, 1678
BssAI RCCGGY 2 cut(s) 611, 856
BssECI CCNNGG 3 cut(s) 136, 492, 594
BssHII GCGCGC 1 cut(s) 1470
BssMI GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
BssNAI GTATAC 1 cut(s) 1969
BssNI GRCGYC 4 cut(s) 114, 141, 488, 513
BssT1I CCWWGG 2 cut(s) 492, 594
Bst1107I GTATAC 1 cut(s) 1969
Bst4CI ACNGT 5 cut(s) 683, 1307, 1524, 1623, 1727
Bst6I CTCTTC 2 cut(s) 109, 467
BstACI GRCGYC 4 cut(s) 114, 141, 488, 513
BstAPI GCANNNNNTGC 2 cut(s) 1375, 2033
BstBI TTCGAA 1 cut(s) 1039
BstC8I GCNNGC 5 cut(s) 191, 858, 1112, 1468, 1472
BstDEI CTNAG 4 cut(s) 129, 882, 964, 1890
BstDSI CCRYGG 2 cut(s) 492, 594
BstF5I GGATG 4 cut(s) 177, 826, 1009, 1882
BstFNI CGCG 4 cut(s) 445, 542, 568, 1472
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 143, 544, 1472, 1474
BstKTI GATC 9 cut(s) 7, 89, 379, 676, 993, 1261, 1327, 1582, 1669
BstMAI GTCTC 4 cut(s) 218, 244, 458, 473
BstMBI GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
BstMCI CGRYCG 2 cut(s) 247, 406
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 2 cut(s) 1379, 1649
BstUI CGCG 4 cut(s) 445, 542, 568, 1472
BstV2I GAAGAC 1 cut(s) 762
BstX2I RGATCY 2 cut(s) 4, 376
BstYI RGATCY 2 cut(s) 4, 376
BstZ17I GTATAC 1 cut(s) 1969
Bsu36I CCTNAGG 1 cut(s) 129
BsuI GTATCC 2 cut(s) 1605, 1739
BsuRI GGCC 7 cut(s) 174, 303, 500, 590, 650, 734, 833
BtgI CCRYGG 2 cut(s) 492, 594
BtgZI GCGATG 1 cut(s) 1533
BtsCI GGATG 4 cut(s) 177, 826, 1009, 1882
BtsIMutI CAGTG 3 cut(s) 688, 1312, 1521
BveI ACCTGC 1 cut(s) 1771
Cac8I GCNNGC 5 cut(s) 191, 858, 1112, 1468, 1472
CaiI CAGNNNCTG 1 cut(s) 2033
CciI TCATGA 1 cut(s) 676
CfoI GCGC 4 cut(s) 143, 544, 1472, 1474
Cfr10I RCCGGY 2 cut(s) 611, 856
Cfr13I GGNCC 3 cut(s) 133, 340, 498
CseI GACGC 5 cut(s) 122, 477, 521, 548, 1140
Csp6I GTAC 8 cut(s) 288, 322, 400, 942, 1180, 1303, 1525, 1936
CspCI CAANNNNNGTGG 2 cut(s) 894, 929
CviAII CATG 8 cut(s) 157, 388, 493, 595, 677, 1463, 1958, 2017
CviQI GTAC 8 cut(s) 288, 322, 400, 942, 1180, 1303, 1525, 1936
DdeI CTNAG 4 cut(s) 129, 882, 964, 1890
DinI GGCGCC 1 cut(s) 142
DpnI GATC 9 cut(s) 6, 88, 378, 675, 992, 1260, 1326, 1581, 1668
DpnII GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
DrdI GACNNNNNNGTC 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 517
DseDI GACNNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 5 cut(s) 172, 301, 588, 648, 831
Eam1104I CTCTTC 2 cut(s) 109, 467
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 2 cut(s) 109, 467
EciI GGCGGA 2 cut(s) 140, 409
Eco130I CCWWGG 2 cut(s) 492, 594
Eco47I GGWCC 2 cut(s) 133, 340
Eco57I CTGAAG 1 cut(s) 1331
Eco81I CCTNAGG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 553
EcoO109I RGGNCCY 3 cut(s) 133, 340, 498
EcoT14I CCWWGG 2 cut(s) 492, 594
EcoT22I ATGCAT 1 cut(s) 1881
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 492, 594
Esp3I CGTCTC 2 cut(s) 458, 473
FaeI CATG 8 cut(s) 160, 391, 496, 598, 680, 1466, 1961, 2020
FalI AAGNNNNNCTT 2 cut(s) 1237, 1269
FaqI GGGAC 2 cut(s) 119, 125
FatI CATG 8 cut(s) 156, 387, 492, 594, 676, 1462, 1957, 2016
FauI CCCGC 7 cut(s) 98, 194, 198, 284, 608, 853, 1697
FbaI TGATCA 1 cut(s) 1579
FblI GTMKAC 3 cut(s) 537, 1655, 1968
FokI GGATG 4 cut(s) 164, 833, 1016, 1869
FspBI CTAG 2 cut(s) 693, 1209
GlaI GCGC 4 cut(s) 142, 543, 1471, 1473
GsaI CCCAGC 1 cut(s) 1981
GsuI CTGGAG 3 cut(s) 951, 1412, 2007
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 7 cut(s) 174, 303, 500, 590, 650, 734, 833
HapII CCGG 6 cut(s) 138, 476, 612, 834, 857, 1121
HgaI GACGC 5 cut(s) 122, 477, 521, 548, 1140
HhaI GCGC 4 cut(s) 143, 544, 1472, 1474
Hin1I GRCGYC 4 cut(s) 114, 141, 488, 513
Hin1II CATG 8 cut(s) 160, 391, 496, 598, 680, 1466, 1961, 2020
Hin6I GCGC 4 cut(s) 141, 542, 1470, 1472
HinP1I GCGC 4 cut(s) 141, 542, 1470, 1472
HincII GTYRAC 2 cut(s) 538, 609
HindII GTYRAC 2 cut(s) 538, 609
HindIII AAGCTT 1 cut(s) 889
HpaII CCGG 6 cut(s) 138, 476, 612, 834, 857, 1121
HphI GGTGA 6 cut(s) 355, 986, 1110, 1512, 1562, 1752
Hpy166II GTNNAC 6 cut(s) 538, 609, 961, 1656, 1792, 1969
Hpy188III TCNNGA 9 cut(s) 331, 380, 677, 707, 930, 966, 1187, 1269, 1292
Hpy8I GTNNAC 6 cut(s) 538, 609, 961, 1656, 1792, 1969
HpyAV CCTTC 7 cut(s) 319, 1125, 1845, 1896, 1917, 1962, 2010
HpyCH4III ACNGT 5 cut(s) 683, 1307, 1524, 1623, 1727
HpyCH4IV ACGT 2 cut(s) 213, 752
HpyF3I CTNAG 4 cut(s) 129, 882, 964, 1890
HpySE526I ACGT 2 cut(s) 213, 752
Hsp92I GRCGYC 4 cut(s) 114, 141, 488, 513
Hsp92II CATG 8 cut(s) 160, 391, 496, 598, 680, 1466, 1961, 2020
HspAI GCGC 4 cut(s) 141, 542, 1470, 1472
KasI GGCGCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 856
KroNI GCCGGC 1 cut(s) 858
Ksp22I TGATCA 1 cut(s) 1579
Kzo9I GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
LmnI GCTCC 8 cut(s) 153, 278, 333, 453, 871, 932, 1416, 1546
LweI GCATC 6 cut(s) 552, 1240, 1432, 1599, 1888, 1990
MaeI CTAG 2 cut(s) 693, 1209
MaeII ACGT 2 cut(s) 213, 752
MaeIII GTNAC 4 cut(s) 361, 1518, 1804, 1865
MalI GATC 9 cut(s) 6, 88, 378, 675, 992, 1260, 1326, 1581, 1668
MboI GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
MboII GAAGA 5 cut(s) 96, 454, 457, 762, 765
MflI RGATCY 2 cut(s) 4, 376
MluCI AATT 8 cut(s) 50, 793, 804, 1338, 1454, 1575, 1820, 1856
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 8 cut(s) 68, 95, 220, 341, 533, 575, 1774, 2014
MmeI TCCRAC 7 cut(s) 53, 267, 288, 603, 1438, 1694, 1863
Mph1103I ATGCAT 1 cut(s) 1881
MroNI GCCGGC 1 cut(s) 856
MseI TTAA 1 cut(s) 1080
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 2033
MspI CCGG 6 cut(s) 138, 476, 612, 834, 857, 1121
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 715
MvnI CGCG 4 cut(s) 445, 542, 568, 1472
NaeI GCCGGC 1 cut(s) 858
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 492, 594
NdeII GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
NgoMIV GCCGGC 1 cut(s) 856
NlaIII CATG 8 cut(s) 160, 391, 496, 598, 680, 1466, 1961, 2020
NlaIV GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1267
NmeAIII GCCGAG 2 cut(s) 142, 563
NmuCI GTSAC 3 cut(s) 361, 1518, 1804
NsiI ATGCAT 1 cut(s) 1881
NspV TTCGAA 1 cut(s) 1039
PagI TCATGA 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 1771
PauI GCGCGC 1 cut(s) 1470
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 219, 378, 519, 773
PctI GAATGC 1 cut(s) 715
PdiI GCCGGC 1 cut(s) 858
PfeI GAWTC 6 cut(s) 65, 623, 728, 1124, 1295, 2060
Pfl23II CGTACG 1 cut(s) 1935
PleI GAGTC 8 cut(s) 68, 95, 220, 341, 532, 575, 1774, 2014
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 8 cut(s) 68, 95, 220, 341, 532, 575, 1774, 2014
PpuMI RGGWCCY 2 cut(s) 133, 340
Psp5II RGGWCCY 2 cut(s) 133, 340
PspFI CCCAGC 1 cut(s) 1977
PspLI CGTACG 1 cut(s) 1935
PspN4I GGNNCC 6 cut(s) 6, 135, 142, 449, 499, 1267
PspPI GGNCC 3 cut(s) 133, 340, 498
PspPPI RGGWCCY 2 cut(s) 133, 340
PstI CTGCAG 1 cut(s) 1653
PstNI CAGNNNCTG 1 cut(s) 2033
PsuI RGATCY 2 cut(s) 4, 376
PteI GCGCGC 1 cut(s) 1470
RsaI GTAC 8 cut(s) 289, 323, 401, 943, 1181, 1304, 1526, 1937
RsaNI GTAC 8 cut(s) 288, 322, 400, 942, 1180, 1303, 1525, 1936
RseI CAYNNNNRTG 1 cut(s) 681
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 1080
Sau3AI GATC 9 cut(s) 4, 86, 376, 673, 990, 1258, 1324, 1579, 1666
Sau96I GGNCC 3 cut(s) 133, 340, 498
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 8 cut(s) 68, 95, 220, 341, 533, 575, 1774, 2014
ScrFI CCNGG 1 cut(s) 138
SfaNI GCATC 6 cut(s) 552, 1240, 1432, 1599, 1888, 1990
SfcI CTRYAG 2 cut(s) 1379, 1649
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 1039
SgrDI CGTCGACG 1 cut(s) 536
SinI GGWCC 2 cut(s) 133, 340
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 8 cut(s) 50, 793, 804, 1338, 1454, 1575, 1820, 1856
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 1486
SspMI CTAG 2 cut(s) 693, 1209
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 492, 594
TaaI ACNGT 5 cut(s) 683, 1307, 1524, 1623, 1727
TaiI ACGT 2 cut(s) 216, 755
TaqI TCGA 9 cut(s) 72, 345, 381, 507, 537, 719, 1039, 1169, 1685
TasI AATT 8 cut(s) 50, 793, 804, 1338, 1454, 1575, 1820, 1856
TatI WGTACW 2 cut(s) 941, 1179
TfiI GAWTC 6 cut(s) 65, 623, 728, 1124, 1295, 2060
Tru1I TTAA 1 cut(s) 1080
Tru9I TTAA 1 cut(s) 1080
TscAI CASTG 3 cut(s) 688, 1312, 1521
TseFI GTSAC 3 cut(s) 361, 1518, 1804
Tsp45I GTSAC 3 cut(s) 361, 1518, 1804
TspDTI ATGAA 5 cut(s) 998, 1496, 1556, 1974, 2073
TspGWI ACGGA 3 cut(s) 349, 350, 440
TspRI CASTG 3 cut(s) 688, 1312, 1521
VpaK11BI GGWCC 2 cut(s) 133, 340
XapI RAATTY 2 cut(s) 793, 1338
XmiI GTMKAC 3 cut(s) 537, 1655, 1968
XspI CTAG 2 cut(s) 693, 1209
ZrmI AGTACT 1 cut(s) 943
Zsp2I ATGCAT 1 cut(s) 1881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.