Rh7AG023200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0011787
Physical Location & Seq
Forward (+)
1543617 .. 1546632
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG023200.1

Sequence Viewer

Length: 2100 bp
ATGGATCCACCGCCTCTCCCACCGCTTCCGGCCACCACGGCCGCCACGCAACTCGGCTACCCCGATTCACTCGACTCCTCCCCCCGATCCCGCAATGCCGACTCTTCTTGGGACGCCGAGCCTCTCCCTCAGGTCCCCGGCGCCAAGCTCCGCCTCATGTGCAGCTATGGCGGCCACATCCTCCCCCGCCCGCACGACAAGTCGCTCTGCTACGTCGGCGGCGAGACTCGCATTGTCGTCGCCGAACGCCACTCCTCCCTCTCCGACCTCTGCTCCCGCCTCTCCCGTACTCTCCTCAACGGCCGCCCCTTCTCTCTCAAGTACCAGCTCCCGACGGAGGACCTCGACTCCCTCATCTCCGTCACCACCGACGAGGATCTCGACAACATGGTGGAGGAGTACGACCGCACCTCCTCCTCCGCCTCGCCGTTGAAGCCGTCGTCGCGGCTTCGTCTCTTTCTCTTCTTTAGCAAACCGGAGACTGCGGCGTCCATGGGGGCCTTGCTCGACGACGCCAAGTCCGAGTCGTGGTTCGTCGACGCGCTCAACGGCTCGGGTCTGATGCCGCGAAACCTCTCTGACTCGGCGGCCACCATGGACTGCTTGTTGACCGGCGGGAGTGATTCTTGCAACGACTTGGAGGCGGCGGCGGCGGCGAGTCAAGGCGACTTCTTTGGGGATCATGACAGTGGAAAACCTAGTGTGGTGGTTCAAGAAGTGCATTCGATTTCGGAATCGGCCTTTGTGGAGAGCAATACGTCGTCCTCTGGGTCGTCTTCTTCGTCGCCTTGCTTGTCAAATTTGCCGCCAATTAGGGTTAGAATGGACGGAAATGTGGCCGGAGTAGTGCAGGGTCAGAAGCCGGCTGGGATTGAGGAGCAGTTTGCTCAGATAAGCTTTGCTGCTGCACCAACAATGGTGGCTGCTGCTGGTTCTGGAGCTTTGAGTACTAATCCTATGGCTGTGAACTCAGGAGGAGGTGAAACTATGAACAGGATCAACTCGGAGGATGATATTTCTGGTCAGAATGTGCCTGTGGGTTTTCGAAAGCCGCCTTTGCCAATGCCATTGCAGCCGCAGCATTTTAAGCAGGGAGGTGGAGGTGGAGGTTATAGCTTGCCTTCACCGGATTCAGTTGCAAGTGATAGTAGCATTGCGTCTGCAAACTCTCTGTCGAAACCAATGTACTATCAAGAGCAAGTTCAAGTTGCAGCTAGAGATGGCAGAGCTCCTGTTAGCCCGAATACAACAAGTGATGCTTCGGATCAGGGTTCCCGAAACCAAGTTCAACAAGTTCAGGATTCAGGGTACAGTGTGCCTCCGCAACCAGATCAACAACAGCAAATTCAGCTTCAGCAACAACAGCAGCAACAGCAACAACTGCTACAGCAGCAGCAGCAGCAACAACAACAACAGCTCCAACAGCAGCATCTCCAGCAGCAGCAACAGCAGCAGCAACAATTTGTCCATGCAAGCGCGCAATATATGAATATTCAGCACCCAGCTACTGGTCAAGTGCCAGTGTCACCGTACTACACAATGTATGCTCCACCATCGCAGCAGCAACAGCTTCATCACCAAATTGATCATCAGCAGCAGTATCCAGTCTATTTGATGCCTGTTGCACAGTCGCAACAACCATACAAGATGGCTCTGCAGTCTACAATAGCTGATCCAGCTGGTGTTGCCTCAACCCGCATTACATCCCAAAACCCTGCTGCTTACAAGGACAGTATCCCACCCATTTATCCCCCAAAGACAGCTTCACCTGCTATGCCTGAAATGACTCAAAGTGTTTACCAGACCTCTGTGACACCAACTCCTCAATTAGTCCAAATCCCTTCCAACCAATATCAGCAGCAATTTGTTGGTTACTCTCAAATGCATCATCCTTCTCAGTCCATTGCTGTTCCTTCCTCTGCGACAGCACCCAACTATGCGTACGAATATGCCAATCCTTCACATGAACAAGTATACTACACCCAGCATCAAGCAGCCCCATTGCCTTCTCAATATCAATCGATGACTCCAGCGGCCGCCGCAGCAGCAGCAGCAATGTCAGATGAATCAAAGCAGAAAAACCAGATGCAGATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

699

Amino Acids

75.44

Weight (kDa)

5.06

Isoelectric Point (pI)

70.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 65 - 155 1.7e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011787)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18230
fragaria_vesca FvH4_5g16730
malus_domestica MD06G1221200.v1.1 MD14G1230400.v1.1
prunus_persica Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1 Prupe.5G225400_v2.0.a1
pyrus_communis pycom14g19170
rosa_chinensis RchiOBHm_Chr7g0179361
rosa_laevigata RLG00000005362
rosa_multiflora Rmu_co8118088.1_g000001 Rmu_sc0019996.1_g000002
rosa_roxburghii Rroxscaffold_3G00274090
rosa_rugosa Rorug06G0423800.1
rosa_samantha Rh7AG023200 Rh7BG022800 Rh7CG024100 Rh7DG023600
rosa_wichuraiana Rw7G001870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1777
AasI GACNNNNNNGTC 1 cut(s) 487
Acc36I ACCTGC 1 cut(s) 1777
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 1508
AccI GTMKAC 3 cut(s) 537, 1661, 1974
AccII CGCG 4 cut(s) 445, 542, 568, 1478
AclWI GGATC 7 cut(s) 12, 81, 384, 687, 1004, 1272, 1667
AcoI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 837, 2034
AcsI RAATTY 2 cut(s) 799, 1344
AcuI CTGAAG 1 cut(s) 1337
AcyI GRCGYC 4 cut(s) 114, 141, 488, 513
AfaI GTAC 8 cut(s) 289, 323, 401, 949, 1187, 1310, 1532, 1943
AfiI CCNNNNNNNGG 3 cut(s) 337, 528, 1508
AgsI TTSAA 4 cut(s) 433, 713, 1205, 1289
AhdI GACNNNNNGTC 1 cut(s) 517
AjuI GAANNNNNNNTTGG 2 cut(s) 1747, 1779
Alw21I GWGCWC 1 cut(s) 1231
Alw26I GTCTC 3 cut(s) 218, 458, 473
AlwI GGATC 7 cut(s) 12, 81, 384, 687, 1004, 1272, 1667
AlwNI CAGNNNCTG 1 cut(s) 1508
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 9 cut(s) 30, 39, 172, 301, 498, 588, 738, 837, 2034
ApoI RAATTY 2 cut(s) 799, 1344
ArsI GACNNNNNNTTYG 2 cut(s) 1157, 1189
AspLEI GCGC 4 cut(s) 143, 544, 1478, 1480
AspS9I GGNCC 3 cut(s) 133, 340, 498
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 6 cut(s) 355, 992, 1116, 1518, 1568, 1758
AsuII TTCGAA 1 cut(s) 1045
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 133, 340
AxyI CCTNAGG 1 cut(s) 129
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 1231
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 1231
BccI CCATC 3 cut(s) 1214, 1561, 1642
BceAI ACGGC 5 cut(s) 54, 316, 412, 421, 565
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciVI GTATCC 2 cut(s) 1611, 1745
BclI TGATCA 1 cut(s) 1585
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 3 cut(s) 218, 458, 473
BfaI CTAG 2 cut(s) 699, 1215
BfmI CTRYAG 2 cut(s) 1385, 1655
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 1777
BfuI GTATCC 2 cut(s) 1611, 1745
BglI GCCNNNNNGGC 1 cut(s) 38
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 2 cut(s) 133, 340
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 133, 340, 498
BmiI GGNNCC 5 cut(s) 6, 135, 142, 499, 1273
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 7 cut(s) 552, 1246, 1438, 1605, 1894, 1996, 2076
BpiI GAAGAC 1 cut(s) 768
BpmI CTGGAG 3 cut(s) 957, 1418, 2013
Bpu14I TTCGAA 1 cut(s) 1045
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 2021
BsaBI GATNNNNATC 1 cut(s) 2090
BsaHI GRCGYC 4 cut(s) 114, 141, 488, 513
BsaJI CCNNGG 4 cut(s) 36, 136, 492, 594
BsaWI WCCGGW 2 cut(s) 475, 1126
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 528, 1508
Bse118I RCCGGY 2 cut(s) 611, 862
Bse1I ACTGG 3 cut(s) 1513, 1520, 1604
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 6 cut(s) 100, 1067, 1152, 1902, 2000, 2061
Bse8I GATNNNNATC 1 cut(s) 2090
BseCI ATCGAT 1 cut(s) 2021
BseDI CCNNGG 4 cut(s) 36, 136, 492, 594
BseGI GGATG 4 cut(s) 177, 1015, 1703, 1888
BseJI GATNNNNATC 1 cut(s) 2090
BseLI CCNNNNNNNGG 3 cut(s) 337, 528, 1508
BseMI GCAATG 6 cut(s) 100, 1067, 1152, 1902, 2000, 2061
BseMII CTCAG 4 cut(s) 143, 902, 984, 1910
BseNI ACTGG 3 cut(s) 1513, 1520, 1604
BsePI GCGCGC 1 cut(s) 1476
BseRI GAGGAG 9 cut(s) 67, 244, 284, 403, 406, 410, 890, 990, 1812
BseX3I CGGCCG 3 cut(s) 39, 301, 2034
BseYI CCCAGC 3 cut(s) 866, 1501, 1983
BsgI GTGCAG 3 cut(s) 181, 869, 891
Bsh1236I CGCG 4 cut(s) 445, 542, 568, 1478
Bsh1285I CGRYCG 4 cut(s) 42, 304, 406, 2037
BshFI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 740, 839, 2036
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 2021
BsiEI CGRYCG 4 cut(s) 42, 304, 406, 2037
BsiHKAI GWGCWC 1 cut(s) 1231
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 7 cut(s) 29, 138, 476, 612, 840, 863, 1127
BsiWI CGTACG 1 cut(s) 1941
BslFI GGGAC 2 cut(s) 119, 125
BslI CCNNNNNNNGG 3 cut(s) 337, 528, 1508
BsmAI GTCTC 3 cut(s) 218, 458, 473
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 119, 125
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 740, 839, 2036
BsoBI CYCGRG 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 1045
Bsp1286I GDGCHC 1 cut(s) 1231
Bsp19I CCATGG 2 cut(s) 492, 594
BspANI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 740, 839, 2036
BspCNI CTCAG 4 cut(s) 142, 901, 983, 1909
BspDI ATCGAT 1 cut(s) 2021
BspFNI CGCG 4 cut(s) 445, 542, 568, 1478
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 5 cut(s) 6, 135, 142, 499, 1273
BspMAI CTGCAG 1 cut(s) 1659
BspMI ACCTGC 1 cut(s) 1777
BspPI GGATC 7 cut(s) 12, 81, 384, 687, 1004, 1272, 1667
BspT104I TTCGAA 1 cut(s) 1045
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 6 cut(s) 100, 1067, 1152, 1902, 2000, 2061
BsrFI RCCGGY 2 cut(s) 611, 862
BsrI ACTGG 3 cut(s) 1513, 1520, 1604
BssAI RCCGGY 2 cut(s) 611, 862
BssECI CCNNGG 4 cut(s) 36, 136, 492, 594
BssHII GCGCGC 1 cut(s) 1476
BssNAI GTATAC 1 cut(s) 1975
BssNI GRCGYC 4 cut(s) 114, 141, 488, 513
BssT1I CCWWGG 2 cut(s) 492, 594
Bst1107I GTATAC 1 cut(s) 1975
Bst4CI ACNGT 5 cut(s) 689, 1313, 1530, 1629, 1733
Bst6I CTCTTC 2 cut(s) 109, 467
BstACI GRCGYC 4 cut(s) 114, 141, 488, 513
BstAPI GCANNNNNTGC 1 cut(s) 1381
BstBI TTCGAA 1 cut(s) 1045
BstC8I GCNNGC 5 cut(s) 191, 864, 1118, 1474, 1478
BstDEI CTNAG 4 cut(s) 129, 888, 970, 1896
BstDSI CCRYGG 3 cut(s) 36, 492, 594
BstF5I GGATG 4 cut(s) 177, 1015, 1703, 1888
BstFNI CGCG 4 cut(s) 445, 542, 568, 1478
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 4 cut(s) 143, 544, 1478, 1480
BstMAI GTCTC 3 cut(s) 218, 458, 473
BstMCI CGRYCG 4 cut(s) 42, 304, 406, 2037
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 2 cut(s) 1385, 1655
BstUI CGCG 4 cut(s) 445, 542, 568, 1478
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 2 cut(s) 4, 376
BstYI RGATCY 2 cut(s) 4, 376
BstZ17I GTATAC 1 cut(s) 1975
BstZI CGGCCG 3 cut(s) 39, 301, 2034
Bsu15I ATCGAT 1 cut(s) 2021
Bsu36I CCTNAGG 1 cut(s) 129
BsuI GTATCC 2 cut(s) 1611, 1745
BsuRI GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 740, 839, 2036
BsuTUI ATCGAT 1 cut(s) 2021
BtgI CCRYGG 3 cut(s) 36, 492, 594
BtgZI GCGATG 1 cut(s) 1539
BtsCI GGATG 4 cut(s) 177, 1015, 1703, 1888
BtsIMutI CAGTG 3 cut(s) 694, 1318, 1527
BveI ACCTGC 1 cut(s) 1777
Cac8I GCNNGC 5 cut(s) 191, 864, 1118, 1474, 1478
CaiI CAGNNNCTG 1 cut(s) 1508
CciI TCATGA 1 cut(s) 682
CciNI GCGGCCGC 1 cut(s) 2034
CfoI GCGC 4 cut(s) 143, 544, 1478, 1480
Cfr10I RCCGGY 2 cut(s) 611, 862
Cfr13I GGNCC 3 cut(s) 133, 340, 498
ClaI ATCGAT 1 cut(s) 2021
CseI GACGC 5 cut(s) 122, 477, 521, 548, 1146
Csp6I GTAC 8 cut(s) 288, 322, 400, 948, 1186, 1309, 1531, 1942
CspCI CAANNNNNGTGG 2 cut(s) 900, 935
CviAII CATG 7 cut(s) 157, 388, 493, 595, 683, 1469, 1964
CviQI GTAC 8 cut(s) 288, 322, 400, 948, 1186, 1309, 1531, 1942
DdeI CTNAG 4 cut(s) 129, 888, 970, 1896
DinI GGCGCC 1 cut(s) 142
DrdI GACNNNNNNGTC 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 517
DseDI GACNNNNNNGTC 1 cut(s) 487
EaeI YGGCCR 7 cut(s) 30, 39, 172, 301, 588, 837, 2034
EagI CGGCCG 3 cut(s) 39, 301, 2034
Eam1104I CTCTTC 2 cut(s) 109, 467
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 2 cut(s) 109, 467
EciI GGCGGA 2 cut(s) 140, 409
Ecl136II GAGCTC 1 cut(s) 1229
EclXI CGGCCG 3 cut(s) 39, 301, 2034
Eco130I CCWWGG 2 cut(s) 492, 594
Eco24I GRGCYC 1 cut(s) 1231
Eco47I GGWCC 2 cut(s) 133, 340
Eco52I CGGCCG 3 cut(s) 39, 301, 2034
Eco53kI GAGCTC 1 cut(s) 1229
Eco57I CTGAAG 1 cut(s) 1337
Eco81I CCTNAGG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 1229
EcoO109I RGGNCCY 3 cut(s) 133, 340, 498
EcoT14I CCWWGG 2 cut(s) 492, 594
EcoT22I ATGCAT 1 cut(s) 1887
EcoT38I GRGCYC 1 cut(s) 1231
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 492, 594
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 7 cut(s) 160, 391, 496, 598, 686, 1472, 1967
FalI AAGNNNNNCTT 2 cut(s) 1243, 1275
FaqI GGGAC 2 cut(s) 119, 125
FatI CATG 7 cut(s) 156, 387, 492, 594, 682, 1468, 1963
FauI CCCGC 6 cut(s) 98, 194, 198, 284, 608, 1703
FbaI TGATCA 1 cut(s) 1585
FblI GTMKAC 3 cut(s) 537, 1661, 1974
FokI GGATG 4 cut(s) 164, 1022, 1690, 1875
FriOI GRGCYC 1 cut(s) 1231
FspBI CTAG 2 cut(s) 699, 1215
GlaI GCGC 4 cut(s) 142, 543, 1477, 1479
GsaI CCCAGC 3 cut(s) 870, 1505, 1987
GsuI CTGGAG 3 cut(s) 957, 1418, 2013
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 9 cut(s) 32, 41, 174, 303, 500, 590, 740, 839, 2036
HapII CCGG 7 cut(s) 29, 138, 476, 612, 840, 863, 1127
HgaI GACGC 5 cut(s) 122, 477, 521, 548, 1146
HhaI GCGC 4 cut(s) 143, 544, 1478, 1480
Hin1I GRCGYC 4 cut(s) 114, 141, 488, 513
Hin1II CATG 7 cut(s) 160, 391, 496, 598, 686, 1472, 1967
Hin6I GCGC 4 cut(s) 141, 542, 1476, 1478
HinP1I GCGC 4 cut(s) 141, 542, 1476, 1478
HincII GTYRAC 2 cut(s) 538, 609
HindII GTYRAC 2 cut(s) 538, 609
HindIII AAGCTT 1 cut(s) 895
HpaII CCGG 7 cut(s) 29, 138, 476, 612, 840, 863, 1127
HphI GGTGA 6 cut(s) 355, 992, 1116, 1518, 1568, 1758
Hpy166II GTNNAC 6 cut(s) 538, 609, 967, 1662, 1798, 1975
Hpy188III TCNNGA 9 cut(s) 331, 380, 683, 713, 936, 972, 1193, 1275, 1298
Hpy8I GTNNAC 6 cut(s) 538, 609, 967, 1662, 1798, 1975
HpyAV CCTTC 7 cut(s) 319, 1131, 1851, 1902, 1923, 1968, 2016
HpyCH4III ACNGT 5 cut(s) 689, 1313, 1530, 1629, 1733
HpyCH4IV ACGT 2 cut(s) 213, 758
HpyF3I CTNAG 4 cut(s) 129, 888, 970, 1896
HpySE526I ACGT 2 cut(s) 213, 758
Hsp92I GRCGYC 4 cut(s) 114, 141, 488, 513
Hsp92II CATG 7 cut(s) 160, 391, 496, 598, 686, 1472, 1967
HspAI GCGC 4 cut(s) 141, 542, 1476, 1478
KasI GGCGCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 862
KroNI GCCGGC 1 cut(s) 864
Ksp22I TGATCA 1 cut(s) 1585
LmnI GCTCC 8 cut(s) 153, 278, 333, 877, 938, 1234, 1422, 1552
LweI GCATC 7 cut(s) 552, 1246, 1438, 1605, 1894, 1996, 2076
MaeI CTAG 2 cut(s) 699, 1215
MaeII ACGT 2 cut(s) 213, 758
MaeIII GTNAC 4 cut(s) 361, 1524, 1810, 1871
MboII GAAGA 4 cut(s) 96, 454, 768, 771
MflI RGATCY 2 cut(s) 4, 376
MhlI GDGCHC 1 cut(s) 1231
MluCI AATT 8 cut(s) 799, 810, 1344, 1460, 1581, 1826, 1862, 2095
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 9 cut(s) 68, 95, 220, 341, 533, 575, 667, 1780, 2020
MmeI TCCRAC 3 cut(s) 288, 1444, 1869
Mph1103I ATGCAT 1 cut(s) 1887
MroNI GCCGGC 1 cut(s) 862
MseI TTAA 1 cut(s) 1086
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 2 cut(s) 1679, 2033
MspI CCGG 7 cut(s) 29, 138, 476, 612, 840, 863, 1127
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 721
MvnI CGCG 4 cut(s) 445, 542, 568, 1478
NaeI GCCGGC 1 cut(s) 864
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 492, 594
NgoMIV GCCGGC 1 cut(s) 862
NlaIII CATG 7 cut(s) 160, 391, 496, 598, 686, 1472, 1967
NlaIV GGNNCC 5 cut(s) 6, 135, 142, 499, 1273
NmeAIII GCCGAG 3 cut(s) 33, 142, 563
NmuCI GTSAC 3 cut(s) 361, 1524, 1810
NotI GCGGCCGC 1 cut(s) 2034
NsiI ATGCAT 1 cut(s) 1887
NspV TTCGAA 1 cut(s) 1045
PagI TCATGA 1 cut(s) 682
PaqCI CACCTGC 1 cut(s) 1777
PauI GCGCGC 1 cut(s) 1476
PcsI WCGNNNNNNNCGW 5 cut(s) 60, 219, 378, 519, 779
PctI GAATGC 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 864
PfeI GAWTC 6 cut(s) 65, 623, 734, 1130, 1301, 2066
Pfl23II CGTACG 1 cut(s) 1941
PflMI CCANNNNNTGG 1 cut(s) 1508
PleI GAGTC 9 cut(s) 68, 95, 220, 341, 532, 575, 666, 1780, 2020
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 9 cut(s) 68, 95, 220, 341, 532, 575, 666, 1780, 2020
PpuMI RGGWCCY 2 cut(s) 133, 340
Psp124BI GAGCTC 1 cut(s) 1231
Psp5II RGGWCCY 2 cut(s) 133, 340
PspFI CCCAGC 3 cut(s) 866, 1501, 1983
PspLI CGTACG 1 cut(s) 1941
PspN4I GGNNCC 5 cut(s) 6, 135, 142, 499, 1273
PspPI GGNCC 3 cut(s) 133, 340, 498
PspPPI RGGWCCY 2 cut(s) 133, 340
PstI CTGCAG 1 cut(s) 1659
PstNI CAGNNNCTG 1 cut(s) 1508
PsuI RGATCY 2 cut(s) 4, 376
PteI GCGCGC 1 cut(s) 1476
PvuII CAGCTG 1 cut(s) 1679
RsaI GTAC 8 cut(s) 289, 323, 401, 949, 1187, 1310, 1532, 1943
RsaNI GTAC 8 cut(s) 288, 322, 400, 948, 1186, 1309, 1531, 1942
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 1231
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 1086
Sau96I GGNCC 3 cut(s) 133, 340, 498
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 9 cut(s) 68, 95, 220, 341, 533, 575, 667, 1780, 2020
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 1231
SfaNI GCATC 7 cut(s) 552, 1246, 1438, 1605, 1894, 1996, 2076
SfcI CTRYAG 2 cut(s) 1385, 1655
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 1045
SgrDI CGTCGACG 1 cut(s) 536
SinI GGWCC 2 cut(s) 133, 340
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 8 cut(s) 799, 810, 1344, 1460, 1581, 1826, 1862, 2095
SspDI GGCGCC 1 cut(s) 140
SspI AATATT 1 cut(s) 1492
SspMI CTAG 2 cut(s) 699, 1215
SstI GAGCTC 1 cut(s) 1231
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 492, 594
TaaI ACNGT 5 cut(s) 689, 1313, 1530, 1629, 1733
TaiI ACGT 2 cut(s) 216, 761
TaqI TCGA 9 cut(s) 72, 345, 381, 507, 537, 725, 1045, 1175, 2021
TasI AATT 8 cut(s) 799, 810, 1344, 1460, 1581, 1826, 1862, 2095
TatI WGTACW 2 cut(s) 947, 1185
TfiI GAWTC 6 cut(s) 65, 623, 734, 1130, 1301, 2066
Tru1I TTAA 1 cut(s) 1086
Tru9I TTAA 1 cut(s) 1086
TscAI CASTG 3 cut(s) 694, 1318, 1527
TseFI GTSAC 3 cut(s) 361, 1524, 1810
Tsp45I GTSAC 3 cut(s) 361, 1524, 1810
TspDTI ATGAA 5 cut(s) 1004, 1502, 1562, 1980, 2079
TspGWI ACGGA 3 cut(s) 349, 350, 843
TspRI CASTG 3 cut(s) 694, 1318, 1527
Van91I CCANNNNNTGG 1 cut(s) 1508
VpaK11BI GGWCC 2 cut(s) 133, 340
XapI RAATTY 2 cut(s) 799, 1344
XmiI GTMKAC 3 cut(s) 537, 1661, 1974
XspI CTAG 2 cut(s) 699, 1215
ZrmI AGTACT 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 1887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.