MD14G1242200.v1.1

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010115
Physical Location & Seq
Forward (+)
32081137 .. 32087557
6421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1242200.v1.1.491

Sequence Viewer

Length: 3507 bp
ATGGAGGACGACGGACAACCGTCGGATGGAGAAGCAGCAGCATCCGCAGCGGAAAAAACGTCACTCTTCCCCGTCGTACCCGCCTCTCAGCAAATCACAAGCGTCCCGCCGTGGCTTTCTAATACCAGCTTCACCACCCAACTCTCCGCCATAAACGACGCCGTCGCCGCACACTTCAAACCCGAGCCTCCGCCGTCACCGCCCGCGCCGCAAGAACATGAACAGGGGGATGTGCGGTCGCAAGCGCAGCCGTACGAGATGCTGGAGTCTTCTTCTGGATCCGATAGGAGCGACGAGAGAGAGAGGACGAAGAAGAAGAAGAAGAAGAAGAAGAGTAAGAGGAGGAAGAGGAGGAGGAGAGAGAGCTCCGTCGAGAGAGGAAGCGGAGCGTTCGCCGATTATGGGTCAAGGAAGTCCGGTGTTCGGGTTTGGGCCGACTCCGAGACTAAACCTTCCAAAGATTATTACCTCGATTCTCATGGGGACCGTGACAATTTGGCCTTTGGAAGTTTGTACAGAATGGACATTGCTCGCTACAAACCTTATGGTCTTGCTGAGCTATCCGGGTCTGATTTTCGAGGTTTGTCCTGGTGGAACAAGACAGGTTCGACATTGGATAGAGATGCTGATGTTGAAGCTTTGGGTGGGAAACTAAAATCTGCTGGTCGATATTGGTCTGCAAAGTACATGGCTTTGGAACGGCATAAGAACCTAAAGCGTGCTCGTATACTTGTCCCCAGAGACCTTGACATTACAGTTTCCGGAGATTTTATTCCTTTAACAGATGCTCAAACATCTGATGACGAATGTGTTGATGGTGATGATCCACTCTCAAGAACTTCAGTGGTTGAAGAGTCTTGGGAAGATGAAGTGCTGCGCAAAACACGTGAGTTTAACAAACTGACAAGGGAGCACCCCCATGACGAAAAGGTTTGGTTAGATTTTGCAGAGTTCCAAGACAGGGTTGCTGATATGCAACCCCAGAAAGGTGCTCGCTTGCAGATTCTGGAAAAAAAGATTAGCATACTGGAGAAGGCGGCAGAGCTTAACCCAGATAATGAGGAACTGTTGTTTCCTCTTCTAAAGGCTTATCAAAGGAGGGACAACTCTGATGTATTAATTAGTAGATGGGAAAAGATACTTATACAGCATTCAGGAAGTTACAAGTTGTGGAGAGAATTTTTGCGCGTCATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGAAATGAGAAAAATGTACGCACATGCAATCCAGGCTCTAGCTTCTGCTTGCAGAAAGCACATTAGGCAGGTTTGCCAAGCAGAAAACCATCCTCCAGATGCTGCTACAGTTCAACTAGAACTTGGTCTGGTTGATATATTTCTCAGTTTTTGTAGGTTTGAATGGCAGGCTGGCTATCAAGAGTTGGCCACTGCCCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTACTCCTCACCGAACAGAGTAAACAGATACTGTTCGAGCACTTTTGGAACAGTGATGGTGCAAGAGTTGGAGAAGAAGGTGCTCTTGGTTGGTCTACATGGTTGGAGAAAGAGGAAGAGAATAGGCAAAGGGTTATCAGAGAGGAGACTGCACATGATGATGAAGGTGGCTGGACAGGTTGGTCAGAACCACTCGCAAAAAATAAGGAAAATAGTATAGAGACAGAAAAGGAGTCCGAAAGTAATGTAGTGGTTGAGGAGTTTCAAGAAGAACTTGAGGATGAAGATGTCAAGCAAGAAGAAGATACTGAAGCTTTGCTAAAGATGCTAGGGATTGATGTTGATGTTGGTGCTAGTGGTGAGATTAAAGACAGTTCAACCTGGATCAAATGGTCAGAAGAAGAGTTATCAAGAGATTGTGTTCAATGGATGCCTGTTCGTACAAGAGCTGCTGTCTCTCATAATGTTGGTACACCTGATGGAGAAGCAGATGAGCACCTTTCGAGAGTCATAATGTATGAAGATGTCAATGAATACCTGTTTTCTTTGAGCTCATCTGAGGCCCGTTTATCTCTAGTGTTACAATTCGTTGATTTCTTTGGCGGGAAAAGCTCTCAGTGGATATCTACAAACAGCTCCACTTGGGCCGAGAAAGTCCTTAGCTTGGAGGCATTGCCAGATTCTATATTACAAACTTTGAGAAAGGTTCATGATTTTTTAATCAAAACACAAGGCAGTTCAAGTAATTTCAGCTTGGAATCTCTCTTGGGCACCACCAATGACATCTACGGAAGAACAGACATGATGAAATTTCTTCGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTCATTTTGTCCTAGAAGATGCTGCTTTAGTTGCTGAAGAGCTATCTGCTATGAATTTGAATCCTTCCAGCCGTTCAGTTACCCCATGCCGAGCTCTGGCAAAGTTCCTCTTAAAGAGTGACCGTCAGGATGTGTTGCTATGTGGAGTTTATGCAAGAAGGGAGGCTTTTCATGGGAATATTGATCATGCAAGAAGGGTATTTGACATGGCATTGTCATCTATTGAAGGGCTTCCTTTGGAATTACAGTCAAATGCTTCTCTTCTATATTTCTGGTATGCTGAGACGGAGCTTGCTAATGAGCATGGCAATGGGTGTGAATCATCACTACGTGCAATGCATATTTTATTTTGCTTAGGAAGTGGTTCTACGTATAGTCCATATAAATCTCACCCATCAAATTTGCAATTAATGAGAGCACGCCAAGGTTTCAAAGAAAAAATAAGAACGGTACAAATGGCATTGATGCGAGGTGTTATAGATGATCAATCTGTTGCTCTCATATGTTCTGCTGCTTTATTTGAAGAGTTAACCTCTGGATGGGCTGCGGGTATTGAAGTTCTAGATCAGGCTTTTGCATTGGTGCTTCCAGAGAGAAAGAGCCGTAGTTATCAACTTGAATTTATGTTCAGCTTTTATATGAAGATGCTTTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATATTGCAGGGGCTTCAAATATATCCATGCAGCCCTGAACTTTTTAGTGATTTCATCAAAGTTGGCCATCTTTATACGACACCTAATAAACTGCGGTGGGTGTTTGACGACCTCTGTCACAAGAAACCTTCTGTGGTCGTCTGGCTTTTTGCATTGTCATTTGAATTGAGTAAAGGGGGATCCGAGCATAGGATCCGTGGATTATTTGAGAGGGCATTGGCAAATGATAGGTTTCATAACTCAGTTGTTCTTTGGCGATGCTACATTGCATATGAGATGAACATGGCAAGCAATCCTTCAGCGGCGAGGAGAAACTTCTTCCGAGCAATCCATGCCTGCCCATGGTCAAAAAAGCTATGGCTTGATGGTTTTGTGAAATTGAATTCCATCTTATCTGCAAAAGAGCTGTCGGATCTCCAAGAAGTCATGCGGGACAAGGAACTGAATCTGAGGACGGACATTTACGAAATTCTTTTGCAAGATGAGTTTGTATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1169

Amino Acids

133.12

Weight (kDa)

5.5

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 294 - 689 1.2e-77 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1642, 2976
Acc16I TGCGCA 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 1282
AccB1I GGYRCC 1 cut(s) 2231
AccI GTMKAC 2 cut(s) 727, 1556
AccII CGCG 2 cut(s) 206, 1188
AccIII TCCGGA 1 cut(s) 761
AclWI GGATC 9 cut(s) 273, 286, 818, 1853, 3183, 3196, 3196, 3209, 3429
AcoI YGGCCR 2 cut(s) 1410, 3073
AcsI RAATTY 8 cut(s) 1178, 1439, 2270, 2368, 2713, 2933, 3391, 3477
AcuI CTGAAG 4 cut(s) 825, 1791, 2370, 3291
AcvI CACGTG 1 cut(s) 887
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 2645
AfaI GTAC 8 cut(s) 78, 254, 515, 686, 1241, 1903, 1933, 2766
AflIII ACRYGT 1 cut(s) 884
AjiI CACGTC 1 cut(s) 2315
AjnI CCWGG 4 cut(s) 587, 1208, 1254, 1841
AjuI GAANNNNNNNTTGG 2 cut(s) 1530, 1562
Alw26I GTCTC 7 cut(s) 437, 735, 1601, 1676, 1921, 2591, 2962
AlwI GGATC 9 cut(s) 273, 286, 818, 1853, 3183, 3196, 3196, 3209, 3429
AlwNI CAGNNNCTG 3 cut(s) 1006, 1325, 1492
Ama87I CYCGRG 1 cut(s) 182
Aor13HI TCCGGA 1 cut(s) 761
AoxI GGCC 6 cut(s) 432, 498, 1410, 2022, 2106, 3073
ApoI RAATTY 8 cut(s) 1178, 1439, 2270, 2368, 2713, 2933, 3391, 3477
ArsI GACNNNNNNTTYG 2 cut(s) 3401, 3433
AseI ATTAAT 2 cut(s) 1118, 2723
Asp700I GAANNNNTTC 2 cut(s) 2544, 2677
AspLEI GCGC 4 cut(s) 208, 247, 879, 1188
AspS9I GGNCC 4 cut(s) 432, 484, 2023, 2106
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 6 cut(s) 124, 189, 830, 1462, 1832, 2696
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 2234
BalI TGGCCA 2 cut(s) 1412, 3075
BamHI GGATCC 3 cut(s) 278, 3188, 3201
BanI GGYRCC 1 cut(s) 2231
BanII GRGCYC 3 cut(s) 368, 2015, 2410
BarI GAAGNNNNNNTAC 2 cut(s) 2665, 2697
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 1 cut(s) 261
BceAI ACGGC 7 cut(s) 94, 146, 178, 235, 716, 2370, 2901
BciT130I CCWGG 4 cut(s) 589, 1210, 1256, 1843
BclI TGATCA 2 cut(s) 2497, 2797
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 7 cut(s) 437, 735, 1601, 1676, 1921, 2591, 2962
BfaI CTAG 7 cut(s) 1262, 1340, 1790, 1815, 2036, 2327, 2876
BfmI CTRYAG 1 cut(s) 1329
BfuAI ACCTGC 1 cut(s) 1282
BlpI GCTNAGC 1 cut(s) 555
Bme1390I CCNGG 5 cut(s) 565, 589, 1210, 1256, 1843
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 2315
BmgT120I GGNCC 4 cut(s) 432, 484, 2023, 2106
BmiI GGNNCC 5 cut(s) 280, 485, 2233, 3190, 3203
BmrFI CCNGG 5 cut(s) 565, 589, 1210, 1256, 1843
BoxI GACNNNNGTC 2 cut(s) 19, 3123
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 2831, 2863
BpmI CTGGAG 4 cut(s) 284, 1049, 1192, 1302
Bpu10I CCTNAGC 2 cut(s) 2120, 2668
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 817, 1757
BpuMI CCSGG 1 cut(s) 565
BsaAI YACGTR 3 cut(s) 887, 2645, 2685
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 735
BsaJI CCNNGG 4 cut(s) 110, 2737, 3205, 3350
BsaWI WCCGGW 2 cut(s) 416, 761
Bse1I ACTGG 1 cut(s) 1032
Bse3DI GCAATG 5 cut(s) 525, 2132, 2629, 2655, 3273
BseAI TCCGGA 1 cut(s) 761
BseBI CCWGG 4 cut(s) 589, 1210, 1256, 1843
BseDI CCNNGG 4 cut(s) 110, 2737, 3205, 3350
BseGI GGATG 8 cut(s) 31, 41, 235, 1312, 1747, 1896, 2449, 2858
BseMI GCAATG 5 cut(s) 525, 2132, 2629, 2655, 3273
BseMII CTCAG 8 cut(s) 101, 546, 1381, 2010, 2090, 2586, 3264, 3449
BseNI ACTGG 1 cut(s) 1032
BseRI GAGGAG 8 cut(s) 355, 364, 367, 370, 1457, 1619, 1733, 3331
BseSI GKGCMC 1 cut(s) 2234
BseYI CCCAGC 1 cut(s) 3000
BsgI GTGCAG 1 cut(s) 1596
Bsh1236I CGCG 2 cut(s) 206, 1188
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 6 cut(s) 434, 500, 1412, 2024, 2108, 3075
BshNI GGYRCC 1 cut(s) 2231
BsiEI CGRYCG 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 3 cut(s) 417, 564, 762
BsiWI CGTACG 1 cut(s) 252
BslFI GGGAC 5 cut(s) 89, 497, 719, 1115, 3455
BsmAI GTCTC 7 cut(s) 437, 735, 1601, 1676, 1921, 2591, 2962
BsmBI CGTCTC 1 cut(s) 2591
BsmFI GGGAC 5 cut(s) 89, 497, 719, 1115, 3455
BsmI GAATGC 1 cut(s) 1150
BsnI GGCC 6 cut(s) 434, 500, 1412, 2024, 2108, 3075
Bso31I GGTCTC 1 cut(s) 735
BsoBI CYCGRG 1 cut(s) 182
Bsp13I TCCGGA 1 cut(s) 761
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 3350
BspANI GGCC 6 cut(s) 434, 500, 1412, 2024, 2108, 3075
BspCNI CTCAG 8 cut(s) 100, 547, 1380, 2011, 2089, 2587, 3263, 3450
BspEI TCCGGA 1 cut(s) 761
BspFNI CGCG 2 cut(s) 206, 1188
BspHI TCATGA 1 cut(s) 2170
BspLI GGNNCC 5 cut(s) 280, 485, 2233, 3190, 3203
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 9 cut(s) 273, 286, 818, 1853, 3183, 3196, 3196, 3209, 3429
BspQI GCTCTTC 1 cut(s) 2346
BspT107I GGYRCC 1 cut(s) 2231
BspTNI GGTCTC 1 cut(s) 735
BsrDI GCAATG 5 cut(s) 525, 2132, 2629, 2655, 3273
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 1032
BssECI CCNNGG 4 cut(s) 110, 2737, 3205, 3350
BssMI GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
BssNAI GTATAC 1 cut(s) 728
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 2 cut(s) 2737, 3350
Bst1107I GTATAC 1 cut(s) 728
Bst2UI CCWGG 4 cut(s) 589, 1210, 1256, 1843
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 3341
BstAUI TGTACA 1 cut(s) 513
BstBAI YACGTR 3 cut(s) 887, 2645, 2685
BstDSI CCRYGG 3 cut(s) 110, 3205, 3350
BstENI CCTNNNNNAGG 1 cut(s) 1424
BstF5I GGATG 8 cut(s) 31, 41, 235, 1312, 1747, 1896, 2449, 2858
BstFNI CGCG 2 cut(s) 206, 1188
BstHHI GCGC 4 cut(s) 208, 247, 879, 1188
BstKTI GATC 9 cut(s) 281, 826, 1848, 2500, 2800, 2881, 3191, 3204, 3424
BstMAI GTCTC 7 cut(s) 437, 735, 1601, 1676, 1921, 2591, 2962
BstMBI GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
BstMCI CGRYCG 1 cut(s) 239
BstNI CCWGG 4 cut(s) 589, 1210, 1256, 1843
BstNSI RCATGY 1 cut(s) 1250
BstPAI GACNNNNGTC 2 cut(s) 19, 3123
BstSCI CCNGG 5 cut(s) 563, 587, 1208, 1254, 1841
BstSFI CTRYAG 1 cut(s) 1329
BstSLI GKGCMC 1 cut(s) 2234
BstSNI TACGTA 1 cut(s) 2685
BstUI CGCG 2 cut(s) 206, 1188
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 4 cut(s) 278, 3188, 3201, 3421
BstYI RGATCY 4 cut(s) 278, 3188, 3201, 3421
BstZ17I GTATAC 1 cut(s) 728
BsuRI GGCC 6 cut(s) 434, 500, 1412, 2024, 2108, 3075
BtgI CCRYGG 3 cut(s) 110, 3205, 3350
BtgZI GCGATG 1 cut(s) 3280
BtrI CACGTC 1 cut(s) 2315
BtsCI GGATG 8 cut(s) 31, 41, 235, 1312, 1747, 1896, 2449, 2858
BtsI GCAGTG 1 cut(s) 1413
BtsIMutI CAGTG 4 cut(s) 849, 1413, 1519, 2084
BveI ACCTGC 1 cut(s) 1282
CaiI CAGNNNCTG 3 cut(s) 1006, 1325, 1492
CciI TCATGA 1 cut(s) 2170
CfoI GCGC 4 cut(s) 208, 247, 879, 1188
Cfr13I GGNCC 4 cut(s) 432, 484, 2023, 2106
CseI GACGC 3 cut(s) 91, 167, 1177
Csp6I GTAC 8 cut(s) 77, 253, 514, 685, 1240, 1902, 1932, 2765
CviQI GTAC 8 cut(s) 77, 253, 514, 685, 1240, 1902, 1932, 2765
DpnI GATC 9 cut(s) 280, 825, 1847, 2499, 2799, 2880, 3190, 3203, 3423
DpnII GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
DraIII CACNNNGTG 1 cut(s) 2645
DrdI GACNNNNNNGTC 2 cut(s) 1642, 2976
DseDI GACNNNNNNGTC 2 cut(s) 1642, 2976
EaeI YGGCCR 2 cut(s) 1410, 3073
EciI GGCGGA 2 cut(s) 136, 180
Ecl136II GAGCTC 3 cut(s) 366, 2013, 2408
Eco105I TACGTA 1 cut(s) 2685
Eco130I CCWWGG 2 cut(s) 2737, 3350
Eco24I GRGCYC 3 cut(s) 368, 2015, 2410
Eco31I GGTCTC 1 cut(s) 735
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 1 cut(s) 484
Eco53kI GAGCTC 3 cut(s) 366, 2013, 2408
Eco57I CTGAAG 4 cut(s) 825, 1791, 2370, 3291
Eco72I CACGTG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 3 cut(s) 366, 2013, 2408
EcoNI CCTNNNNNAGG 1 cut(s) 1424
EcoRI GAATTC 2 cut(s) 1439, 3391
EcoRII CCWGG 4 cut(s) 587, 1208, 1254, 1841
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 2 cut(s) 2737, 3350
EcoT22I ATGCAT 1 cut(s) 2655
EcoT38I GRGCYC 3 cut(s) 368, 2015, 2410
ErhI CCWWGG 2 cut(s) 2737, 3350
Esp3I CGTCTC 1 cut(s) 2591
FaqI GGGAC 5 cut(s) 89, 497, 719, 1115, 3455
FauI CCCGC 6 cut(s) 88, 114, 211, 2057, 2854, 3432
FauNDI CATATG 2 cut(s) 2816, 3280
FbaI TGATCA 2 cut(s) 2497, 2797
FblI GTMKAC 2 cut(s) 727, 1556
FokI GGATG 8 cut(s) 28, 38, 242, 1299, 1754, 1903, 2456, 2865
FriOI GRGCYC 3 cut(s) 368, 2015, 2410
FspBI CTAG 7 cut(s) 1262, 1340, 1790, 1815, 2036, 2327, 2876
FspI TGCGCA 1 cut(s) 878
GlaI GCGC 4 cut(s) 207, 246, 878, 1187
GsaI CCCAGC 1 cut(s) 3004
GsuI CTGGAG 4 cut(s) 284, 1049, 1192, 1302
HaeIII GGCC 6 cut(s) 434, 500, 1412, 2024, 2108, 3075
HapII CCGG 3 cut(s) 417, 564, 762
HgaI GACGC 3 cut(s) 91, 167, 1177
HhaI GCGC 4 cut(s) 208, 247, 879, 1188
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 4 cut(s) 206, 245, 877, 1186
HinP1I GCGC 4 cut(s) 206, 245, 877, 1186
HincII GTYRAC 1 cut(s) 2844
HindII GTYRAC 1 cut(s) 2844
HindIII AAGCTT 2 cut(s) 636, 1773
HpaI GTTAAC 1 cut(s) 2844
HpaII CCGG 3 cut(s) 417, 564, 762
HphI GGTGA 6 cut(s) 124, 189, 830, 1462, 1832, 2696
Hpy166II GTNNAC 5 cut(s) 728, 1484, 1557, 1934, 2844
Hpy8I GTNNAC 5 cut(s) 728, 1484, 1557, 1934, 2844
Hpy99I CGWCG 7 cut(s) 14, 25, 77, 161, 167, 296, 374
HpyCH4IV ACGT 5 cut(s) 59, 886, 2314, 2644, 2684
HpySE526I ACGT 5 cut(s) 59, 886, 2314, 2644, 2684
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 4 cut(s) 206, 245, 877, 1186
Kpn2I TCCGGA 1 cut(s) 761
Ksp22I TGATCA 2 cut(s) 2497, 2797
KspAI GTTAAC 1 cut(s) 2844
Kzo9I GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
LguI GCTCTTC 1 cut(s) 2346
LmnI GCTCC 6 cut(s) 288, 371, 386, 910, 2102, 2602
MaeI CTAG 7 cut(s) 1262, 1340, 1790, 1815, 2036, 2327, 2876
MaeII ACGT 5 cut(s) 59, 886, 2314, 2644, 2684
MaeIII GTNAC 8 cut(s) 60, 195, 488, 1160, 2040, 2392, 2432, 3125
MalI GATC 9 cut(s) 280, 825, 1847, 2499, 2799, 2880, 3190, 3203, 3423
MboI GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
MflI RGATCY 4 cut(s) 278, 3188, 3201, 3421
MlsI TGGCCA 2 cut(s) 1412, 3075
MluNI TGGCCA 2 cut(s) 1412, 3075
MlyI GAGTC 6 cut(s) 275, 431, 863, 1703, 1977, 3014
MmeI TCCRAC 3 cut(s) 1510, 1545, 3399
Mox20I TGGCCA 2 cut(s) 1412, 3075
Mph1103I ATGCAT 1 cut(s) 2655
MroI TCCGGA 1 cut(s) 761
MroXI GAANNNNTTC 2 cut(s) 2544, 2677
MscI TGGCCA 2 cut(s) 1412, 3075
MseI TTAA 9 cut(s) 779, 894, 1047, 1118, 1827, 2180, 2426, 2723, 2843
MslI CAYNNNNRTG 3 cut(s) 918, 1620, 2622
Msp20I TGGCCA 2 cut(s) 1412, 3075
MspA1I CMGCKG 2 cut(s) 50, 3311
MspI CCGG 3 cut(s) 417, 564, 762
MspR9I CCNGG 5 cut(s) 565, 589, 1210, 1256, 1843
Mva1269I GAATGC 1 cut(s) 1150
MvaI CCWGG 4 cut(s) 589, 1210, 1256, 1843
MvnI CGCG 2 cut(s) 206, 1188
NciI CCSGG 1 cut(s) 565
NcoI CCATGG 1 cut(s) 3350
NdeI CATATG 2 cut(s) 2816, 3280
NdeII GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
NlaIV GGNNCC 5 cut(s) 280, 485, 2233, 3190, 3203
NmeAIII GCCGAG 2 cut(s) 2134, 2429
NmuCI GTSAC 5 cut(s) 60, 195, 488, 2432, 3125
NsbI TGCGCA 1 cut(s) 878
NsiI ATGCAT 1 cut(s) 2655
NspI RCATGY 1 cut(s) 1250
PagI TCATGA 1 cut(s) 2170
PciSI GCTCTTC 1 cut(s) 2346
PctI GAATGC 1 cut(s) 1150
PdmI GAANNNNTTC 2 cut(s) 2544, 2677
PfeI GAWTC 7 cut(s) 473, 1003, 2141, 2219, 2374, 2633, 3454
Pfl23II CGTACG 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 161
PleI GAGTC 6 cut(s) 274, 431, 862, 1702, 1976, 3013
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 6 cut(s) 274, 431, 862, 1702, 1976, 3013
Ppu21I YACGTR 3 cut(s) 887, 2645, 2685
PshAI GACNNNNGTC 2 cut(s) 19, 3123
PshBI ATTAAT 2 cut(s) 1118, 2723
Psp124BI GAGCTC 3 cut(s) 368, 2015, 2410
Psp6I CCWGG 4 cut(s) 587, 1208, 1254, 1841
PspCI CACGTG 1 cut(s) 887
PspFI CCCAGC 1 cut(s) 3000
PspGI CCWGG 4 cut(s) 587, 1208, 1254, 1841
PspLI CGTACG 1 cut(s) 252
PspN4I GGNNCC 5 cut(s) 280, 485, 2233, 3190, 3203
PspPI GGNCC 4 cut(s) 432, 484, 2023, 2106
PstNI CAGNNNCTG 3 cut(s) 1006, 1325, 1492
PsuI RGATCY 4 cut(s) 278, 3188, 3201, 3421
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 8 cut(s) 78, 254, 515, 686, 1241, 1903, 1933, 2766
RsaNI GTAC 8 cut(s) 77, 253, 514, 685, 1240, 1902, 1932, 2765
RseI CAYNNNNRTG 3 cut(s) 918, 1620, 2622
SacI GAGCTC 3 cut(s) 368, 2015, 2410
SapI GCTCTTC 1 cut(s) 2346
SaqAI TTAA 9 cut(s) 779, 894, 1047, 1118, 1827, 2180, 2426, 2723, 2843
Sau3AI GATC 9 cut(s) 278, 823, 1845, 2497, 2797, 2878, 3188, 3201, 3421
Sau96I GGNCC 4 cut(s) 432, 484, 2023, 2106
SchI GAGTC 6 cut(s) 275, 431, 863, 1703, 1977, 3014
ScrFI CCNGG 5 cut(s) 565, 589, 1210, 1256, 1843
SfcI CTRYAG 1 cut(s) 1329
SinI GGWCC 1 cut(s) 484
SmiMI CAYNNNNRTG 3 cut(s) 918, 1620, 2622
SmlI CTYRAG 2 cut(s) 832, 1736
SmoI CTYRAG 2 cut(s) 832, 1736
SnaBI TACGTA 1 cut(s) 2685
SspI AATATT 1 cut(s) 2494
SspMI CTAG 7 cut(s) 1262, 1340, 1790, 1815, 2036, 2327, 2876
SstI GAGCTC 3 cut(s) 368, 2015, 2410
StyD4I CCNGG 5 cut(s) 563, 587, 1208, 1254, 1841
StyI CCWWGG 2 cut(s) 2737, 3350
TaiI ACGT 5 cut(s) 62, 889, 2317, 2647, 2687
TaqI TCGA 7 cut(s) 372, 471, 577, 608, 667, 1497, 1964
TatI WGTACW 2 cut(s) 513, 684
TauI GCSGC 4 cut(s) 170, 211, 1040, 3314
TfiI GAWTC 7 cut(s) 473, 1003, 2141, 2219, 2374, 2633, 3454
Tru1I TTAA 9 cut(s) 779, 894, 1047, 1118, 1827, 2180, 2426, 2723, 2843
Tru9I TTAA 9 cut(s) 779, 894, 1047, 1118, 1827, 2180, 2426, 2723, 2843
TscAI CASTG 4 cut(s) 849, 1420, 1519, 2084
TseFI GTSAC 5 cut(s) 60, 195, 488, 2432, 3125
Tsp45I GTSAC 5 cut(s) 60, 195, 488, 2432, 3125
TspGWI ACGGA 6 cut(s) 27, 358, 2265, 2615, 3194, 3479
TspRI CASTG 4 cut(s) 849, 1420, 1519, 2084
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 484
VspI ATTAAT 2 cut(s) 1118, 2723
XagI CCTNNNNNAGG 1 cut(s) 1424
XapI RAATTY 8 cut(s) 1178, 1439, 2270, 2368, 2713, 2933, 3391, 3477
XbaI TCTAGA 1 cut(s) 2875
XceI RCATGY 1 cut(s) 1250
XmiI GTMKAC 2 cut(s) 727, 1556
XmnI GAANNNNTTC 2 cut(s) 2544, 2677
XspI CTAG 7 cut(s) 1262, 1340, 1790, 1815, 2036, 2327, 2876
Zsp2I ATGCAT 1 cut(s) 2655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.