Rroxscaffold_3G00275680

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010115
Physical Location & Seq
Forward (+)
67047945 .. 67055023
7079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275680.1

Sequence Viewer

Length: 3492 bp
ATGGACGACGAGAGGCCGTCGAAAGAAGAAGCGAAGCCGTCGCCGTCGCTGTTCCCGGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACCTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCTCTATCGCCACCGCCGGAGGAGCAAGAGGACGTACTGCCCCAGCCGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGCGAGCGATGAGAGATACAGGACGAGCAAGAAGAGGAGGAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGGAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCTTGGGCCGAATCCGAGACTAGACCGTCGGAAAATTATTACTTGGATTCCAGTGGCGACCTTGATAATTTGGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGCTCCGGGAATTTTAAAGCTATGTATCAGGGGAATCAGACAATTTCAACATTGGATAGAGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCCGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCCAGAGACCTTGCGGATACAGTTTCAGCTGATTTTATTCCTTTGACGGATATTCAAACATCCGATGAAGGTGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACGAGGGAGCATCCCCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAGCATTCAGGAAATTACAACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTAAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACAGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGTGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTATTCGAGGAATTTCAAGAAGAAACTGAGAATGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGTTGGGGCTACTGGTGAGGGTAAAGACACTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGCGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAGTACCTCTTTTCTTTGAGCTCATGTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTTCTTAGCCTGGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTCTTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTCTGTTGGGGACCATCAATGACATCCATGGAAAGGCAGACCTGATGAAGTTTCTTCGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAGGAGCTATCTGTTGTGAATCTGAATCCATCCTGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGTATCTTTTAAAGAGTGATCGTCAGGATATCTTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCTAGAAGAGTATTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCATCCATCAAATTTGCAATTGCTGAGAGCACGCCTAGGCTTCAAAGAAAGAATAAGAACAGTTCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATGAAGATGCTGTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTCGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCTTCCACATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTCTCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1163

Amino Acids

132.35

Weight (kDa)

5.49

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 287 - 684 7.6e-77 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1071
AasI GACNNNNNNGTC 3 cut(s) 424, 1621, 2961
Acc36I ACCTGC 2 cut(s) 1261, 1360
AccB7I CCANNNNNTGG 1 cut(s) 1387
AccII CGCG 1 cut(s) 344
AclWI GGATC 4 cut(s) 252, 265, 1295, 2674
AcoI YGGCCR 2 cut(s) 1389, 3058
AcsI RAATTY 7 cut(s) 544, 1418, 1700, 2698, 2918, 3306, 3462
AcuI CTGAAG 2 cut(s) 1770, 1911
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 7 cut(s) 213, 233, 659, 1220, 1657, 1982, 2766
AfiI CCNNNNNNNGG 9 cut(s) 375, 396, 940, 965, 1387, 2239, 2395, 3300, 3336
AflII CTTAAG 1 cut(s) 2164
AhdI GACNNNNNGTC 1 cut(s) 358
AjnI CCWGG 5 cut(s) 1187, 1233, 1820, 2109, 3299
AjuI GAANNNNNNNTTGG 8 cut(s) 98, 130, 899, 931, 1356, 1388, 1509, 1541
AloI GAACNNNNNNTCC 2 cut(s) 2841, 2873
Alw21I GWGCWC 6 cut(s) 973, 1482, 1525, 2000, 2395, 2719
Alw26I GTCTC 7 cut(s) 85, 345, 410, 488, 708, 1074, 2947
AlwI GGATC 4 cut(s) 252, 265, 1295, 2674
AlwNI CAGNNNCTG 1 cut(s) 985
Ama87I CYCGRG 2 cut(s) 808, 864
AoxI GGCC 6 cut(s) 14, 405, 471, 1389, 2007, 3058
ApeKI GCWGC 7 cut(s) 521, 791, 955, 1247, 2321, 2792, 2810
ApoI RAATTY 7 cut(s) 544, 1418, 1700, 2698, 2918, 3306, 3462
ArsI GACNNNNNNTTYG 2 cut(s) 3386, 3418
AseI ATTAAT 1 cut(s) 1097
Asp700I GAANNNNTTC 1 cut(s) 2529
AspA2I CCTAGG 1 cut(s) 2722
AspLEI GCGC 2 cut(s) 344, 693
AspS9I GGNCC 5 cut(s) 405, 1533, 2008, 2217, 2547
AsuC2I CCSGG 3 cut(s) 56, 541, 634
AsuHPI GGTGA 3 cut(s) 109, 791, 1811
AsuNHI GCTAGC 1 cut(s) 2590
AvaI CYCGRG 2 cut(s) 808, 864
AvaII GGWCC 3 cut(s) 1533, 2217, 2547
AvrII CCTAGG 1 cut(s) 2722
BalI TGGCCA 2 cut(s) 1391, 3060
BamHI GGATCC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 2000, 3176
BarI GAAGNNNNNNTAC 2 cut(s) 1798, 1830
BbsI GAAGAC 2 cut(s) 1049, 2060
Bbv12I GWGCWC 6 cut(s) 973, 1482, 1525, 2000, 2395, 2719
BbvCI CCTCAGC 1 cut(s) 2580
BbvI GCAGC 7 cut(s) 508, 778, 967, 1234, 2308, 2779, 2797
BceAI ACGGC 6 cut(s) 22, 28, 131, 214, 370, 2373
BcgI CGANNNNNNTGC 4 cut(s) 131, 165, 3170, 3204
BciT130I CCWGG 5 cut(s) 1189, 1235, 1822, 2111, 3301
BciVI GTATCC 1 cut(s) 718
BclI TGATCA 3 cut(s) 1861, 2482, 2782
BcnI CCSGG 3 cut(s) 56, 541, 634
BcoDI GTCTC 7 cut(s) 85, 345, 410, 488, 708, 1074, 2947
BfmI CTRYAG 2 cut(s) 487, 2368
BfoI RGCGCY 1 cut(s) 694
BfrI CTTAAG 1 cut(s) 2164
BfuAI ACCTGC 2 cut(s) 1261, 1360
BfuI GTATCC 1 cut(s) 718
BglII AGATCT 1 cut(s) 3406
BisI GCNGC 8 cut(s) 66, 522, 792, 956, 1248, 2322, 2793, 2811
BlnI CCTAGG 1 cut(s) 2722
BlsI GCNGC 8 cut(s) 67, 523, 793, 957, 1249, 2323, 2794, 2812
Bme1390I CCNGG 8 cut(s) 56, 541, 634, 1189, 1235, 1822, 2111, 3301
Bme18I GGWCC 3 cut(s) 1533, 2217, 2547
BmeRI GACNNNNNGTC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 808, 864
BmgT120I GGNCC 5 cut(s) 405, 1533, 2008, 2217, 2547
BmiI GGNNCC 2 cut(s) 259, 2218
BmrFI CCNGG 8 cut(s) 56, 541, 634, 1189, 1235, 1822, 2111, 3301
BmsI GCATC 7 cut(s) 586, 598, 901, 1755, 1860, 2934, 3242
BmtI GCTAGC 1 cut(s) 2594
BoxI GACNNNNGTC 1 cut(s) 3108
BpiI GAAGAC 2 cut(s) 1049, 2060
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 4 cut(s) 801, 1171, 1281, 2132
Bpu10I CCTNAGC 3 cut(s) 168, 2580, 2653
BpuMI CCSGG 3 cut(s) 56, 541, 634
BsaAI YACGTR 2 cut(s) 2300, 2768
BsaBI GATNNNNATC 1 cut(s) 1860
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 5 cut(s) 633, 1279, 2233, 2722, 3335
Bsc4I CCNNNNNNNGG 9 cut(s) 375, 396, 940, 965, 1387, 2239, 2395, 3300, 3336
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 4 cut(s) 390, 450, 818, 1801
Bse3DI GCAATG 5 cut(s) 498, 2117, 2640, 2883, 3258
Bse8I GATNNNNATC 1 cut(s) 1860
BseBI CCWGG 5 cut(s) 1189, 1235, 1822, 2111, 3301
BseDI CCNNGG 5 cut(s) 633, 1279, 2233, 2722, 3335
BseJI GATNNNNATC 1 cut(s) 1860
BseLI CCNNNNNNNGG 9 cut(s) 375, 396, 940, 965, 1387, 2239, 2395, 3300, 3336
BseMI GCAATG 5 cut(s) 498, 2117, 2640, 2883, 3258
BseNI ACTGG 4 cut(s) 390, 450, 818, 1801
BseRI GAGGAG 7 cut(s) 212, 313, 337, 1436, 1598, 2351, 2547
BseXI GCAGC 7 cut(s) 508, 778, 967, 1234, 2308, 2779, 2797
BseYI CCCAGC 2 cut(s) 219, 2985
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 6 cut(s) 16, 407, 473, 1391, 2009, 3060
BsiHKAI GWGCWC 6 cut(s) 973, 1482, 1525, 2000, 2395, 2719
BsiHKCI CYCGRG 2 cut(s) 808, 864
BsiSI CCGG 5 cut(s) 56, 194, 369, 540, 633
BsiWI CGTACG 1 cut(s) 231
BslFI GGGAC 3 cut(s) 74, 1930, 2230
BslI CCNNNNNNNGG 9 cut(s) 375, 396, 940, 965, 1387, 2239, 2395, 3300, 3336
BsmAI GTCTC 7 cut(s) 85, 345, 410, 488, 708, 1074, 2947
BsmBI CGTCTC 1 cut(s) 345
BsmFI GGGAC 3 cut(s) 74, 1930, 2230
BsmI GAATGC 2 cut(s) 1129, 1263
BsnI GGCC 6 cut(s) 16, 407, 473, 1391, 2009, 3060
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 2 cut(s) 808, 864
Bsp1286I GDGCHC 7 cut(s) 973, 1482, 1525, 2000, 2395, 2719, 3176
Bsp19I CCATGG 2 cut(s) 2233, 3335
BspANI GGCC 6 cut(s) 16, 407, 473, 1391, 2009, 3060
BspFNI CGCG 1 cut(s) 344
BspHI TCATGA 1 cut(s) 2155
BspLI GGNNCC 2 cut(s) 259, 2218
BspMAI CTGCAG 1 cut(s) 2372
BspMI ACCTGC 2 cut(s) 1261, 1360
BspOI GCTAGC 1 cut(s) 2594
BspPI GGATC 4 cut(s) 252, 265, 1295, 2674
BspTI CTTAAG 1 cut(s) 2164
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 5 cut(s) 498, 2117, 2640, 2883, 3258
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 4 cut(s) 390, 450, 818, 1801
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 5 cut(s) 633, 1279, 2233, 2722, 3335
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 4 cut(s) 1279, 2233, 2722, 3335
Bst2UI CCWGG 5 cut(s) 1189, 1235, 1822, 2111, 3301
Bst4CI ACNGT 9 cut(s) 426, 730, 1047, 1085, 1493, 2031, 2081, 2749, 3110
Bst6I CTCTTC 7 cut(s) 290, 314, 326, 2452, 2488, 2565, 2817
BstACI GRCGYC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 2164
BstBAI YACGTR 2 cut(s) 2300, 2768
BstC8I GCNNGC 8 cut(s) 268, 973, 977, 1252, 2592, 2719, 3283, 3331
BstDSI CCRYGG 2 cut(s) 2233, 3335
BstFNI CGCG 1 cut(s) 344
BstH2I RGCGCY 1 cut(s) 694
BstHHI GCGC 2 cut(s) 344, 693
BstMAI GTCTC 7 cut(s) 85, 345, 410, 488, 708, 1074, 2947
BstNI CCWGG 5 cut(s) 1189, 1235, 1822, 2111, 3301
BstNSI RCATGY 4 cut(s) 1169, 1229, 1254, 3285
BstPAI GACNNNNGTC 1 cut(s) 3108
BstSCI CCNGG 8 cut(s) 54, 539, 632, 1187, 1233, 1820, 2109, 3299
BstSFI CTRYAG 2 cut(s) 487, 2368
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 7 cut(s) 508, 778, 967, 1234, 2308, 2779, 2797
BstV2I GAAGAC 2 cut(s) 1049, 2060
BstX2I RGATCY 3 cut(s) 257, 1300, 3406
BstXI CCANNNNNNTGG 1 cut(s) 1543
BstYI RGATCY 3 cut(s) 257, 1300, 3406
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 6 cut(s) 16, 407, 473, 1391, 2009, 3060
BtgI CCRYGG 2 cut(s) 2233, 3335
BtgZI GCGATG 2 cut(s) 285, 3265
BtsIMutI CAGTG 5 cut(s) 397, 457, 1449, 1498, 2069
BveI ACCTGC 2 cut(s) 1261, 1360
Cac8I GCNNGC 8 cut(s) 268, 973, 977, 1252, 2592, 2719, 3283, 3331
CaiI CAGNNNCTG 1 cut(s) 985
CciI TCATGA 1 cut(s) 2155
CfoI GCGC 2 cut(s) 344, 693
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 5 cut(s) 405, 1533, 2008, 2217, 2547
CseI GACGC 1 cut(s) 152
Csp6I GTAC 7 cut(s) 212, 232, 658, 1219, 1656, 1981, 2765
CviQI GTAC 7 cut(s) 212, 232, 658, 1219, 1656, 1981, 2765
DraI TTTAAA 3 cut(s) 550, 2412, 2683
DrdI GACNNNNNNGTC 3 cut(s) 424, 1621, 2961
DriI GACNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 3 cut(s) 424, 1621, 2961
EaeI YGGCCR 2 cut(s) 1389, 3058
Eam1104I CTCTTC 7 cut(s) 290, 314, 326, 2452, 2488, 2565, 2817
Eam1105I GACNNNNNGTC 1 cut(s) 358
EarI CTCTTC 7 cut(s) 290, 314, 326, 2452, 2488, 2565, 2817
EciI GGCGGA 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 1998
Eco130I CCWWGG 4 cut(s) 1279, 2233, 2722, 3335
Eco24I GRGCYC 2 cut(s) 2000, 3176
Eco31I GGTCTC 1 cut(s) 708
Eco32I GATATC 2 cut(s) 2070, 2431
Eco47I GGWCC 3 cut(s) 1533, 2217, 2547
Eco53kI GAGCTC 1 cut(s) 1998
Eco57I CTGAAG 2 cut(s) 1770, 1911
Eco88I CYCGRG 2 cut(s) 808, 864
EcoICRI GAGCTC 1 cut(s) 1998
EcoRI GAATTC 1 cut(s) 1418
EcoRII CCWGG 5 cut(s) 1187, 1233, 1820, 2109, 3299
EcoRV GATATC 2 cut(s) 2070, 2431
EcoT14I CCWWGG 4 cut(s) 1279, 2233, 2722, 3335
EcoT22I ATGCAT 2 cut(s) 613, 2640
EcoT38I GRGCYC 2 cut(s) 2000, 3176
ErhI CCWWGG 4 cut(s) 1279, 2233, 2722, 3335
Esp3I CGTCTC 1 cut(s) 345
FalI AAGNNNNNCTT 6 cut(s) 1509, 1541, 1929, 1961, 2449, 2481
FaqI GGGAC 3 cut(s) 74, 1930, 2230
FauI CCCGC 4 cut(s) 350, 1312, 1907, 3417
FauNDI CATATG 1 cut(s) 3265
FbaI TGATCA 3 cut(s) 1861, 2482, 2782
Fnu4HI GCNGC 8 cut(s) 66, 522, 792, 956, 1248, 2322, 2793, 2811
FriOI GRGCYC 2 cut(s) 2000, 3176
Fsp4HI GCNGC 8 cut(s) 66, 522, 792, 956, 1248, 2322, 2793, 2811
GlaI GCGC 2 cut(s) 343, 692
GluI GCNGC 8 cut(s) 66, 522, 792, 956, 1248, 2322, 2793, 2811
GsaI CCCAGC 2 cut(s) 223, 2989
GsuI CTGGAG 4 cut(s) 801, 1171, 1281, 2132
HaeII RGCGCY 1 cut(s) 694
HaeIII GGCC 6 cut(s) 16, 407, 473, 1391, 2009, 3060
HapII CCGG 5 cut(s) 56, 194, 369, 540, 633
HgaI GACGC 1 cut(s) 152
HhaI GCGC 2 cut(s) 344, 693
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 2 cut(s) 342, 691
HinP1I GCGC 2 cut(s) 342, 691
HincII GTYRAC 1 cut(s) 2829
HindII GTYRAC 1 cut(s) 2829
HindIII AAGCTT 1 cut(s) 1752
HpaI GTTAAC 1 cut(s) 2829
HpaII CCGG 5 cut(s) 56, 194, 369, 540, 633
HphI GGTGA 3 cut(s) 109, 791, 1811
Hpy166II GTNNAC 4 cut(s) 623, 701, 1536, 2829
Hpy8I GTNNAC 4 cut(s) 623, 701, 1536, 2829
Hpy99I CGWCG 6 cut(s) 11, 22, 43, 49, 146, 430
HpyCH4III ACNGT 9 cut(s) 426, 730, 1047, 1085, 1493, 2031, 2081, 2749, 3110
HpyCH4IV ACGT 5 cut(s) 87, 210, 2299, 2767, 3074
HpySE526I ACGT 5 cut(s) 87, 210, 2299, 2767, 3074
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 2 cut(s) 342, 691
Ksp22I TGATCA 3 cut(s) 1861, 2482, 2782
KspAI GTTAAC 1 cut(s) 2829
LmnI GCTCC 8 cut(s) 199, 339, 542, 889, 1449, 2338, 2560, 2587
Lsp1109I GCAGC 7 cut(s) 508, 778, 967, 1234, 2308, 2779, 2797
LweI GCATC 7 cut(s) 586, 598, 901, 1755, 1860, 2934, 3242
MaeII ACGT 5 cut(s) 87, 210, 2299, 2767, 3074
MaeIII GTNAC 3 cut(s) 801, 2025, 2377
MfeI CAATTG 1 cut(s) 2705
MflI RGATCY 3 cut(s) 257, 1300, 3406
MhlI GDGCHC 7 cut(s) 973, 1482, 1525, 2000, 2395, 2719, 3176
MlsI TGGCCA 2 cut(s) 1391, 3060
MluNI TGGCCA 2 cut(s) 1391, 3060
MlyI GAGTC 5 cut(s) 809, 856, 976, 1962, 2999
MmeI TCCRAC 5 cut(s) 129, 408, 1489, 1524, 1592
Mox20I TGGCCA 2 cut(s) 1391, 3060
Mph1103I ATGCAT 2 cut(s) 613, 2640
MroXI GAANNNNTTC 1 cut(s) 2529
MscI TGGCCA 2 cut(s) 1391, 3060
MslI CAYNNNNRTG 4 cut(s) 897, 1599, 2062, 2942
Msp20I TGGCCA 2 cut(s) 1391, 3060
MspA1I CMGCKG 2 cut(s) 737, 2792
MspCI CTTAAG 1 cut(s) 2164
MspI CCGG 5 cut(s) 56, 194, 369, 540, 633
MspR9I CCNGG 8 cut(s) 56, 541, 634, 1189, 1235, 1822, 2111, 3301
MunI CAATTG 1 cut(s) 2705
Mva1269I GAATGC 2 cut(s) 1129, 1263
MvaI CCWGG 5 cut(s) 1189, 1235, 1822, 2111, 3301
MvnI CGCG 1 cut(s) 344
NciI CCSGG 3 cut(s) 56, 541, 634
NcoI CCATGG 2 cut(s) 2233, 3335
NdeI CATATG 1 cut(s) 3265
NheI GCTAGC 1 cut(s) 2590
NlaIV GGNNCC 2 cut(s) 259, 2218
NmuCI GTSAC 1 cut(s) 801
NsiI ATGCAT 2 cut(s) 613, 2640
NspI RCATGY 4 cut(s) 1169, 1229, 1254, 3285
PaeI GCATGC 2 cut(s) 1254, 3285
PagI TCATGA 1 cut(s) 2155
PctI GAATGC 2 cut(s) 1129, 1263
PdmI GAANNNNTTC 1 cut(s) 2529
Pfl23II CGTACG 1 cut(s) 231
PflMI CCANNNNNTGG 1 cut(s) 1387
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 8 cut(s) 67, 523, 793, 957, 1249, 2323, 2794, 2812
PleI GAGTC 5 cut(s) 808, 856, 976, 1961, 2998
PpsI GAGTC 5 cut(s) 808, 856, 976, 1961, 2998
Ppu21I YACGTR 2 cut(s) 2300, 2768
PshAI GACNNNNGTC 1 cut(s) 3108
PshBI ATTAAT 1 cut(s) 1097
PsiI TTATAA 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 2000
Psp6I CCWGG 5 cut(s) 1187, 1233, 1820, 2109, 3299
PspFI CCCAGC 2 cut(s) 219, 2985
PspGI CCWGG 5 cut(s) 1187, 1233, 1820, 2109, 3299
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 259, 2218
PspPI GGNCC 5 cut(s) 405, 1533, 2008, 2217, 2547
PstI CTGCAG 1 cut(s) 2372
PstNI CAGNNNCTG 1 cut(s) 985
PsuI RGATCY 3 cut(s) 257, 1300, 3406
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 7 cut(s) 213, 233, 659, 1220, 1657, 1982, 2766
RsaNI GTAC 7 cut(s) 212, 232, 658, 1219, 1656, 1981, 2765
RseI CAYNNNNRTG 4 cut(s) 897, 1599, 2062, 2942
SacI GAGCTC 1 cut(s) 2000
SatI GCNGC 8 cut(s) 66, 522, 792, 956, 1248, 2322, 2793, 2811
Sau96I GGNCC 5 cut(s) 405, 1533, 2008, 2217, 2547
SchI GAGTC 5 cut(s) 809, 856, 976, 1962, 2999
ScrFI CCNGG 8 cut(s) 56, 541, 634, 1189, 1235, 1822, 2111, 3301
SduI GDGCHC 7 cut(s) 973, 1482, 1525, 2000, 2395, 2719, 3176
SfaNI GCATC 7 cut(s) 586, 598, 901, 1755, 1860, 2934, 3242
SfcI CTRYAG 2 cut(s) 487, 2368
SinI GGWCC 3 cut(s) 1533, 2217, 2547
SmiMI CAYNNNNRTG 4 cut(s) 897, 1599, 2062, 2942
SmlI CTYRAG 1 cut(s) 2164
SmoI CTYRAG 1 cut(s) 2164
SphI GCATGC 2 cut(s) 1254, 3285
SspI AATATT 1 cut(s) 2479
SstI GAGCTC 1 cut(s) 2000
StyD4I CCNGG 8 cut(s) 54, 539, 632, 1187, 1233, 1820, 2109, 3299
StyI CCWWGG 4 cut(s) 1279, 2233, 2722, 3335
TaaI ACNGT 9 cut(s) 426, 730, 1047, 1085, 1493, 2031, 2081, 2749, 3110
TaiI ACGT 5 cut(s) 90, 213, 2302, 2770, 3077
TaqI TCGA 6 cut(s) 20, 380, 505, 828, 1695, 1949
TaqII GACCGA 1 cut(s) 2601
TatI WGTACW 1 cut(s) 657
TauI GCSGC 1 cut(s) 68
TscAI CASTG 5 cut(s) 397, 457, 1456, 1498, 2069
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 7 cut(s) 521, 791, 955, 1247, 2321, 2792, 2810
Tsp45I GTSAC 1 cut(s) 801
TspGWI ACGGA 3 cut(s) 121, 770, 2667
TspRI CASTG 5 cut(s) 397, 457, 1456, 1498, 2069
Van91I CCANNNNNTGG 1 cut(s) 1387
Vha464I CTTAAG 1 cut(s) 2164
VpaK11BI GGWCC 3 cut(s) 1533, 2217, 2547
VspI ATTAAT 1 cut(s) 1097
XapI RAATTY 7 cut(s) 544, 1418, 1700, 2698, 2918, 3306, 3462
XbaI TCTAGA 1 cut(s) 2860
XceI RCATGY 4 cut(s) 1169, 1229, 1254, 3285
XmaJI CCTAGG 1 cut(s) 2722
XmnI GAANNNNTTC 1 cut(s) 2529
Zsp2I ATGCAT 2 cut(s) 613, 2640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.