RLG00000005516

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010115
Physical Location & Seq
Forward (+)
68252200 .. 68259090
6891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005516

Sequence Viewer

Length: 3486 bp
ATGGACGACAAGCCGTCGGAAGAAGAAGCAAAGCCGTCGCCGTCTCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACCTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCGCCGCCTCTATCGCCACCGCCGGAGGACCAAGAGGACGTACGGCCCCAGCTGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGCGAGCAATCATAGAGACACGACGAGCAAGAAGAGGAGGAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGGAGAGAGAGGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCTTGGGCCGAATCCGAGACTAGACCGTTGGAAAATTATTACTTGGATTCCAATGGCGACCTTGATAATTTGGCCTTCGGATGTCTCTATAGGATGGATATTGCTCGATACAAACCATATGCTGCTCTTTCGGATAGCTCCGGGAATTTTAAAGCTTTGTATCGGGGGAATCAGACAGGTTCAACATTGGATAGGGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCTGGGGGTCGCTATTGGTCTGCAAAGTACACGGCGTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCCAGAGACCTTCCGGATACAGTTTTGGGTGATTTTGTTCCTTTGACGGATATTCAAACATCCGATGAAGGTGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTTGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACGAGGGAGCATCCTCATGATGAAAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTGCTGAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAGCATTCAGGAAATTACAACTTGTGGAGAGAGTTTCTGCGTGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATGCTGCTATTGTTCAACTAGAACTTGGTCTGGTTGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTTTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTTCTCACTGAGCAGAGTAAGCAGATATTGTTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCGTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTTATCAGAGAGGAGATGGCACATCATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAAGCTGAAAGTAATGCTGTATTTGAGGAATTTCAAGAAGAAACTGAGAATGAAGATATCAAGCAGGAAGAAGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGGTGGGGCTACTGGTGAGGTTAAAGACACTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTTTGATCAATGGATGCCTGTTCGCGCAAAATCTGAAGCTGTTGCCTCTAATAATGGTGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAATACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTGGAGGCATTGCCACAGTCAGTATTACAGACTCTGAAAAGGGTTCATGATGTCTTAAGCAAAAGACAAGGTAGTTCAAGTAGTTTCAGCTTGGAATCTCTCTTGGGGACCAATGACATCCATGGGAAGGCAGACCTGATGAAGTTTCTTCGTAATGCTACCTTGCTTTGTTTATCTGCTTTTCCACGTAATTATATCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTGAATCTGAATCCATCCTGCAGTTCAGTTACCCCCTGCCGTGGTCTGGCAAAGTATCTTTTAAAAAGTGATCGTCAGGATGTATTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCAAGAAGAGTATTTGACTTGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAATGATCATGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTCTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCCTTTAAATGTCAGCCATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAGAGAAAGAATAAGAACAGTTCAATTGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCAGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATGAAGATGCTGTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTACAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCTAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAGGAAACCATCTGTTGTCGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAAGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATAGCATATGAAATGAACATAGCATGCAATCCTTCCACATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCTTGGTCGAAGAAGCTATGGCTCGATGGTTTTCTCAAATTGAGCTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACGGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1162

Amino Acids

132.16

Weight (kDa)

5.55

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 286 - 683 2e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1068
AasI GACNNNNNNGTC 2 cut(s) 1618, 2955
Acc36I ACCTGC 2 cut(s) 1258, 1357
AccB7I CCANNNNNTGG 1 cut(s) 1384
AccII CGCG 2 cut(s) 341, 1878
AccIII TCCGGA 1 cut(s) 718
AclWI GGATC 3 cut(s) 249, 262, 2668
AcoI YGGCCR 2 cut(s) 1386, 3052
AcsI RAATTY 7 cut(s) 541, 1415, 1697, 2692, 2912, 3300, 3456
AcuI CTGAAG 5 cut(s) 242, 1079, 1767, 1908, 2707
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 7 cut(s) 210, 230, 656, 1217, 1654, 2760, 3480
AflII CTTAAG 1 cut(s) 2161
AhdI GACNNNNNGTC 1 cut(s) 13
AjnI CCWGG 5 cut(s) 1184, 1230, 1817, 2106, 3293
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 1353, 1385
AloI GAACNNNNNNTCC 2 cut(s) 2835, 2867
Alw21I GWGCWC 5 cut(s) 970, 1479, 1997, 2713, 3374
Alw26I GTCTC 8 cut(s) 48, 82, 270, 407, 485, 705, 1071, 2941
AlwI GGATC 3 cut(s) 249, 262, 2668
AlwNI CAGNNNCTG 3 cut(s) 982, 1301, 2140
Ama87I CYCGRG 2 cut(s) 805, 861
Aor13HI TCCGGA 1 cut(s) 718
AoxI GGCC 6 cut(s) 212, 402, 468, 1386, 2004, 3052
ApeKI GCWGC 8 cut(s) 518, 788, 952, 1244, 1301, 2315, 2786, 2804
ApoI RAATTY 7 cut(s) 541, 1415, 1697, 2692, 2912, 3300, 3456
ArsI GACNNNNNNTTYG 2 cut(s) 3380, 3412
AseI ATTAAT 1 cut(s) 1094
Asp700I GAANNNNTTC 1 cut(s) 2523
AspLEI GCGC 4 cut(s) 341, 690, 1520, 1880
AspS9I GGNCC 7 cut(s) 196, 213, 402, 1530, 2005, 2214, 2541
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 4 cut(s) 106, 746, 788, 1808
AsuNHI GCTAGC 1 cut(s) 2584
AvaI CYCGRG 2 cut(s) 805, 861
AvaII GGWCC 4 cut(s) 196, 1530, 2214, 2541
BalI TGGCCA 2 cut(s) 1388, 3054
BamHI GGATCC 1 cut(s) 254
BanII GRGCYC 3 cut(s) 1997, 3170, 3374
BauI CACGAG 1 cut(s) 1521
BbsI GAAGAC 1 cut(s) 2057
Bbv12I GWGCWC 5 cut(s) 970, 1479, 1997, 2713, 3374
BbvCI CCTCAGC 1 cut(s) 2574
BbvI GCAGC 8 cut(s) 505, 775, 964, 1231, 1288, 2302, 2773, 2816
BceAI ACGGC 7 cut(s) 19, 25, 128, 211, 227, 675, 2367
BcgI CGANNNNNNTGC 6 cut(s) 18, 52, 128, 162, 3164, 3198
BciT130I CCWGG 5 cut(s) 1186, 1232, 1819, 2108, 3295
BciVI GTATCC 1 cut(s) 715
BclI TGATCA 4 cut(s) 1858, 2476, 2593, 2776
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 8 cut(s) 48, 82, 270, 407, 485, 705, 1071, 2941
BfmI CTRYAG 2 cut(s) 484, 2362
BfoI RGCGCY 2 cut(s) 691, 1521
BfrI CTTAAG 1 cut(s) 2161
BfuAI ACCTGC 2 cut(s) 1258, 1357
BfuI GTATCC 1 cut(s) 715
BglII AGATCT 1 cut(s) 3400
BmcAI AGTACT 1 cut(s) 3480
Bme1390I CCNGG 6 cut(s) 538, 1186, 1232, 1819, 2108, 3295
Bme18I GGWCC 4 cut(s) 196, 1530, 2214, 2541
BmeRI GACNNNNNGTC 1 cut(s) 13
BmeT110I CYCGRG 2 cut(s) 805, 861
BmgT120I GGNCC 7 cut(s) 196, 213, 402, 1530, 2005, 2214, 2541
BmiI GGNNCC 3 cut(s) 215, 256, 2215
BmrFI CCNGG 6 cut(s) 538, 1186, 1232, 1819, 2108, 3295
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 8 cut(s) 583, 595, 898, 1288, 1752, 1857, 2928, 3236
BmtI GCTAGC 1 cut(s) 2588
BmuI ACTGGG 1 cut(s) 47
BoxI GACNNNNGTC 1 cut(s) 3102
BpiI GAAGAC 1 cut(s) 2057
BplI GAGNNNNNCTC 2 cut(s) 788, 820
BpmI CTGGAG 4 cut(s) 798, 1168, 1278, 2129
Bpu10I CCTNAGC 4 cut(s) 165, 2102, 2574, 2647
BpuMI CCSGG 1 cut(s) 538
BsaAI YACGTR 2 cut(s) 2294, 2762
BsaBI GATNNNNATC 1 cut(s) 1857
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 5 cut(s) 1276, 2227, 2383, 2716, 3329
BsaWI WCCGGW 1 cut(s) 718
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 4 cut(s) 53, 387, 815, 1798
Bse3DI GCAATG 3 cut(s) 2114, 2634, 2877
Bse8I GATNNNNATC 1 cut(s) 1857
BseAI TCCGGA 1 cut(s) 718
BseBI CCWGG 5 cut(s) 1186, 1232, 1819, 2108, 3295
BseDI CCNNGG 5 cut(s) 1276, 2227, 2383, 2716, 3329
BseJI GATNNNNATC 1 cut(s) 1857
BseMI GCAATG 3 cut(s) 2114, 2634, 2877
BseNI ACTGG 4 cut(s) 53, 387, 815, 1798
BseRI GAGGAG 4 cut(s) 310, 334, 1595, 2541
BseXI GCAGC 8 cut(s) 505, 775, 964, 1231, 1288, 2302, 2773, 2816
BseYI CCCAGC 2 cut(s) 216, 2979
BsgI GTGCAG 1 cut(s) 2823
Bsh1236I CGCG 2 cut(s) 341, 1878
BshFI GGCC 6 cut(s) 214, 404, 470, 1388, 2006, 3054
BsiHKAI GWGCWC 5 cut(s) 970, 1479, 1997, 2713, 3374
BsiHKCI CYCGRG 2 cut(s) 805, 861
BsiSI CCGG 4 cut(s) 191, 366, 537, 719
BsiWI CGTACG 2 cut(s) 208, 228
BslFI GGGAC 4 cut(s) 71, 1927, 2227, 2655
BsmAI GTCTC 8 cut(s) 48, 82, 270, 407, 485, 705, 1071, 2941
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 4 cut(s) 71, 1927, 2227, 2655
BsmI GAATGC 2 cut(s) 1126, 1260
BsnI GGCC 6 cut(s) 214, 404, 470, 1388, 2006, 3054
Bso31I GGTCTC 1 cut(s) 705
BsoBI CYCGRG 2 cut(s) 805, 861
Bsp1286I GDGCHC 6 cut(s) 970, 1479, 1997, 2713, 3170, 3374
Bsp13I TCCGGA 1 cut(s) 718
Bsp19I CCATGG 1 cut(s) 2227
BspANI GGCC 6 cut(s) 214, 404, 470, 1388, 2006, 3054
BspEI TCCGGA 1 cut(s) 718
BspFNI CGCG 2 cut(s) 341, 1878
BspHI TCATGA 2 cut(s) 895, 2152
BspLI GGNNCC 3 cut(s) 215, 256, 2215
BspMAI CTGCAG 1 cut(s) 2366
BspMI ACCTGC 2 cut(s) 1258, 1357
BspOI GCTAGC 1 cut(s) 2588
BspPI GGATC 3 cut(s) 249, 262, 2668
BspQI GCTCTTC 1 cut(s) 2325
BspTI CTTAAG 1 cut(s) 2161
BspTNI GGTCTC 1 cut(s) 705
BsrDI GCAATG 3 cut(s) 2114, 2634, 2877
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 4 cut(s) 53, 387, 815, 1798
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 5 cut(s) 1276, 2227, 2383, 2716, 3329
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 1521
BssT1I CCWWGG 4 cut(s) 1276, 2227, 2716, 3329
Bst2BI CACGAG 1 cut(s) 1521
Bst2UI CCWGG 5 cut(s) 1186, 1232, 1819, 2108, 3295
Bst6I CTCTTC 8 cut(s) 287, 311, 323, 2325, 2446, 2482, 2559, 2811
BstACI GRCGYC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 2161
BstBAI YACGTR 2 cut(s) 2294, 2762
BstC8I GCNNGC 8 cut(s) 265, 970, 974, 1249, 2586, 2713, 3277, 3325
BstDSI CCRYGG 2 cut(s) 2227, 2383
BstFNI CGCG 2 cut(s) 341, 1878
BstH2I RGCGCY 2 cut(s) 691, 1521
BstHHI GCGC 4 cut(s) 341, 690, 1520, 1880
BstMAI GTCTC 8 cut(s) 48, 82, 270, 407, 485, 705, 1071, 2941
BstNI CCWGG 5 cut(s) 1186, 1232, 1819, 2108, 3295
BstNSI RCATGY 3 cut(s) 1226, 1251, 3279
BstPAI GACNNNNGTC 1 cut(s) 3102
BstSCI CCNGG 6 cut(s) 536, 1184, 1230, 1817, 2106, 3293
BstSFI CTRYAG 2 cut(s) 484, 2362
BstUI CGCG 2 cut(s) 341, 1878
BstV1I GCAGC 8 cut(s) 505, 775, 964, 1231, 1288, 2302, 2773, 2816
BstV2I GAAGAC 1 cut(s) 2057
BstX2I RGATCY 2 cut(s) 254, 3400
BstXI CCANNNNNNTGG 1 cut(s) 1540
BstYI RGATCY 2 cut(s) 254, 3400
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 6 cut(s) 214, 404, 470, 1388, 2006, 3054
BtgI CCRYGG 2 cut(s) 2227, 2383
BtgZI GCGATG 1 cut(s) 3259
BtsIMutI CAGTG 5 cut(s) 394, 1446, 1495, 2066, 3040
BveI ACCTGC 2 cut(s) 1258, 1357
Cac8I GCNNGC 8 cut(s) 265, 970, 974, 1249, 2586, 2713, 3277, 3325
CaiI CAGNNNCTG 3 cut(s) 982, 1301, 2140
CciI TCATGA 2 cut(s) 895, 2152
CfoI GCGC 4 cut(s) 341, 690, 1520, 1880
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 7 cut(s) 196, 213, 402, 1530, 2005, 2214, 2541
CseI GACGC 1 cut(s) 149
Csp6I GTAC 7 cut(s) 209, 229, 655, 1216, 1653, 2759, 3479
CviQI GTAC 7 cut(s) 209, 229, 655, 1216, 1653, 2759, 3479
DraI TTTAAA 3 cut(s) 547, 2406, 2677
DrdI GACNNNNNNGTC 2 cut(s) 1618, 2955
DriI GACNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 2 cut(s) 1618, 2955
EaeI YGGCCR 2 cut(s) 1386, 3052
Eam1104I CTCTTC 8 cut(s) 287, 311, 323, 2325, 2446, 2482, 2559, 2811
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 8 cut(s) 287, 311, 323, 2325, 2446, 2482, 2559, 2811
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 2 cut(s) 1995, 3372
Eco130I CCWWGG 4 cut(s) 1276, 2227, 2716, 3329
Eco24I GRGCYC 3 cut(s) 1997, 3170, 3374
Eco31I GGTCTC 1 cut(s) 705
Eco32I GATATC 2 cut(s) 1726, 2067
Eco47I GGWCC 4 cut(s) 196, 1530, 2214, 2541
Eco53kI GAGCTC 2 cut(s) 1995, 3372
Eco57I CTGAAG 5 cut(s) 242, 1079, 1767, 1908, 2707
Eco88I CYCGRG 2 cut(s) 805, 861
EcoICRI GAGCTC 2 cut(s) 1995, 3372
EcoRI GAATTC 1 cut(s) 1415
EcoRII CCWGG 5 cut(s) 1184, 1230, 1817, 2106, 3293
EcoRV GATATC 2 cut(s) 1726, 2067
EcoT14I CCWWGG 4 cut(s) 1276, 2227, 2716, 3329
EcoT22I ATGCAT 2 cut(s) 610, 2634
EcoT38I GRGCYC 3 cut(s) 1997, 3170, 3374
ErhI CCWWGG 4 cut(s) 1276, 2227, 2716, 3329
Esp3I CGTCTC 1 cut(s) 48
FalI AAGNNNNNCTT 4 cut(s) 1926, 1958, 2443, 2475
FaqI GGGAC 4 cut(s) 71, 1927, 2227, 2655
FauI CCCGC 2 cut(s) 347, 3411
FauNDI CATATG 2 cut(s) 514, 3259
FbaI TGATCA 4 cut(s) 1858, 2476, 2593, 2776
FriOI GRGCYC 3 cut(s) 1997, 3170, 3374
GlaI GCGC 4 cut(s) 340, 689, 1519, 1879
GsaI CCCAGC 2 cut(s) 220, 2983
GsuI CTGGAG 4 cut(s) 798, 1168, 1278, 2129
HaeII RGCGCY 2 cut(s) 691, 1521
HaeIII GGCC 6 cut(s) 214, 404, 470, 1388, 2006, 3054
HapII CCGG 4 cut(s) 191, 366, 537, 719
HgaI GACGC 1 cut(s) 149
HhaI GCGC 4 cut(s) 341, 690, 1520, 1880
Hin1I GRCGYC 1 cut(s) 141
Hin6I GCGC 4 cut(s) 339, 688, 1518, 1878
HinP1I GCGC 4 cut(s) 339, 688, 1518, 1878
HincII GTYRAC 1 cut(s) 2823
HindII GTYRAC 1 cut(s) 2823
HindIII AAGCTT 2 cut(s) 549, 1749
HpaI GTTAAC 1 cut(s) 2823
HpaII CCGG 4 cut(s) 191, 366, 537, 719
HphI GGTGA 4 cut(s) 106, 746, 788, 1808
Hpy166II GTNNAC 5 cut(s) 620, 657, 698, 1533, 2823
Hpy8I GTNNAC 5 cut(s) 620, 657, 698, 1533, 2823
Hpy99I CGWCG 4 cut(s) 19, 40, 143, 286
HpyCH4IV ACGT 5 cut(s) 84, 207, 2293, 2761, 3068
HpySE526I ACGT 5 cut(s) 84, 207, 2293, 2761, 3068
Hsp92I GRCGYC 1 cut(s) 141
HspAI GCGC 4 cut(s) 339, 688, 1518, 1878
Kpn2I TCCGGA 1 cut(s) 718
Ksp22I TGATCA 4 cut(s) 1858, 2476, 2593, 2776
KspAI GTTAAC 1 cut(s) 2823
LguI GCTCTTC 1 cut(s) 2325
LmnI GCTCC 6 cut(s) 336, 539, 886, 2554, 2581, 3377
Lsp1109I GCAGC 8 cut(s) 505, 775, 964, 1231, 1288, 2302, 2773, 2816
LweI GCATC 8 cut(s) 583, 595, 898, 1288, 1752, 1857, 2928, 3236
MaeII ACGT 5 cut(s) 84, 207, 2293, 2761, 3068
MaeIII GTNAC 3 cut(s) 798, 2022, 2371
MfeI CAATTG 2 cut(s) 2699, 2747
MflI RGATCY 2 cut(s) 254, 3400
MhlI GDGCHC 6 cut(s) 970, 1479, 1997, 2713, 3170, 3374
MlsI TGGCCA 2 cut(s) 1388, 3054
MluNI TGGCCA 2 cut(s) 1388, 3054
MlyI GAGTC 6 cut(s) 806, 853, 973, 1959, 2131, 2993
MmeI TCCRAC 6 cut(s) 126, 405, 645, 1486, 1521, 1589
Mox20I TGGCCA 2 cut(s) 1388, 3054
Mph1103I ATGCAT 2 cut(s) 610, 2634
MroI TCCGGA 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 2523
MscI TGGCCA 2 cut(s) 1388, 3054
MslI CAYNNNNRTG 4 cut(s) 894, 1596, 2059, 2936
Msp20I TGGCCA 2 cut(s) 1388, 3054
MspA1I CMGCKG 2 cut(s) 220, 2786
MspCI CTTAAG 1 cut(s) 2161
MspI CCGG 4 cut(s) 191, 366, 537, 719
MspR9I CCNGG 6 cut(s) 538, 1186, 1232, 1819, 2108, 3295
MunI CAATTG 2 cut(s) 2699, 2747
Mva1269I GAATGC 2 cut(s) 1126, 1260
MvaI CCWGG 5 cut(s) 1186, 1232, 1819, 2108, 3295
MvnI CGCG 2 cut(s) 341, 1878
NciI CCSGG 1 cut(s) 538
NcoI CCATGG 1 cut(s) 2227
NdeI CATATG 2 cut(s) 514, 3259
NheI GCTAGC 1 cut(s) 2584
NlaIV GGNNCC 3 cut(s) 215, 256, 2215
NmuCI GTSAC 1 cut(s) 798
NsiI ATGCAT 2 cut(s) 610, 2634
NspI RCATGY 3 cut(s) 1226, 1251, 3279
PaeI GCATGC 2 cut(s) 1251, 3279
PagI TCATGA 2 cut(s) 895, 2152
PciSI GCTCTTC 1 cut(s) 2325
PctI GAATGC 2 cut(s) 1126, 1260
PdmI GAANNNNTTC 1 cut(s) 2523
Pfl23II CGTACG 2 cut(s) 208, 228
PflMI CCANNNNNTGG 1 cut(s) 1384
PfoI TCCNGGA 1 cut(s) 536
PleI GAGTC 6 cut(s) 805, 853, 973, 1958, 2131, 2992
PpsI GAGTC 6 cut(s) 805, 853, 973, 1958, 2131, 2992
Ppu21I YACGTR 2 cut(s) 2294, 2762
PshAI GACNNNNGTC 1 cut(s) 3102
PshBI ATTAAT 1 cut(s) 1094
PsiI TTATAA 1 cut(s) 1068
Psp124BI GAGCTC 2 cut(s) 1997, 3374
Psp6I CCWGG 5 cut(s) 1184, 1230, 1817, 2106, 3293
PspFI CCCAGC 2 cut(s) 216, 2979
PspGI CCWGG 5 cut(s) 1184, 1230, 1817, 2106, 3293
PspLI CGTACG 2 cut(s) 208, 228
PspN4I GGNNCC 3 cut(s) 215, 256, 2215
PspPI GGNCC 7 cut(s) 196, 213, 402, 1530, 2005, 2214, 2541
PstI CTGCAG 1 cut(s) 2366
PstNI CAGNNNCTG 3 cut(s) 982, 1301, 2140
PsuI RGATCY 2 cut(s) 254, 3400
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 7 cut(s) 210, 230, 656, 1217, 1654, 2760, 3480
RsaNI GTAC 7 cut(s) 209, 229, 655, 1216, 1653, 2759, 3479
RseI CAYNNNNRTG 4 cut(s) 894, 1596, 2059, 2936
SacI GAGCTC 2 cut(s) 1997, 3374
SapI GCTCTTC 1 cut(s) 2325
Sau96I GGNCC 7 cut(s) 196, 213, 402, 1530, 2005, 2214, 2541
ScaI AGTACT 1 cut(s) 3480
SchI GAGTC 6 cut(s) 806, 853, 973, 1959, 2131, 2993
ScrFI CCNGG 6 cut(s) 538, 1186, 1232, 1819, 2108, 3295
SduI GDGCHC 6 cut(s) 970, 1479, 1997, 2713, 3170, 3374
SfaNI GCATC 8 cut(s) 583, 595, 898, 1288, 1752, 1857, 2928, 3236
SfcI CTRYAG 2 cut(s) 484, 2362
SinI GGWCC 4 cut(s) 196, 1530, 2214, 2541
SmiMI CAYNNNNRTG 4 cut(s) 894, 1596, 2059, 2936
SmlI CTYRAG 1 cut(s) 2161
SmoI CTYRAG 1 cut(s) 2161
SphI GCATGC 2 cut(s) 1251, 3279
SspI AATATT 1 cut(s) 2473
SstI GAGCTC 2 cut(s) 1997, 3374
StyD4I CCNGG 6 cut(s) 536, 1184, 1230, 1817, 2106, 3293
StyI CCWWGG 4 cut(s) 1276, 2227, 2716, 3329
TaiI ACGT 5 cut(s) 87, 210, 2296, 2764, 3071
TaqI TCGA 5 cut(s) 377, 502, 1946, 3335, 3351
TaqII GACCGA 1 cut(s) 2595
TatI WGTACW 2 cut(s) 654, 3478
TauI GCSGC 3 cut(s) 65, 172, 175
TscAI CASTG 5 cut(s) 394, 1453, 1495, 2066, 3040
TseFI GTSAC 1 cut(s) 798
TseI GCWGC 8 cut(s) 518, 788, 952, 1244, 1301, 2315, 2786, 2804
Tsp45I GTSAC 1 cut(s) 798
TspGWI ACGGA 3 cut(s) 118, 767, 3458
TspRI CASTG 5 cut(s) 394, 1453, 1495, 2066, 3040
Van91I CCANNNNNTGG 1 cut(s) 1384
Vha464I CTTAAG 1 cut(s) 2161
VpaK11BI GGWCC 4 cut(s) 196, 1530, 2214, 2541
VspI ATTAAT 1 cut(s) 1094
XapI RAATTY 7 cut(s) 541, 1415, 1697, 2692, 2912, 3300, 3456
XbaI TCTAGA 1 cut(s) 2854
XceI RCATGY 3 cut(s) 1226, 1251, 3279
XmnI GAANNNNTTC 1 cut(s) 2523
ZrmI AGTACT 1 cut(s) 3480
Zsp2I ATGCAT 2 cut(s) 610, 2634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.