Rh7DG007900

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0010115
Physical Location & Seq
Reverse (-)
654294 .. 661093
6800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG007900.1

Sequence Viewer

Length: 3453 bp
ATGGACGACGACAGGCCGTCGCCGTCGCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACCTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCACCGCCGGAGGAGCAAGACGACGTACGGCCCCAGCCGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGGTCCGAAGCGAGCGATGAGAGAGACAGGACGAGCAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGCAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCATGGGCCGAATCCGAGACTAGACCGCCGTCGGAAAATTATTACTTGGATTCCAGTGGCGACCTTGATAATTTGGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGCTCTGGGAATTTTAAAGCTTTGTATCAGGGGAATCAGACAATTTCAACATTGGATAGAGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCCGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCTAGAGACCTTGCGGATACAGTTTCAGGTGATTTTATTCCTTTGACGGATATTCAAACATCCGATGAAGGTGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGCTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACGAGGGAGCATCCTCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAGCATTCAGGAAATTACCACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCCGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTCAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTCTATTTCAGGCTGAAATTGAGTTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTACTTGAGGAATTTCAAGAAGAAACTGAGAACGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGTTGGGGCTACTGGTGAGGGTAAAGACGCTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGCGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAGTACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTGGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTCTTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTCTGTTGGGGACCACCAATGACATCCATGGACAGGCAGACCTGATGAAGTTTCTTTGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTCAATCTGAATCCATCCCGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGTATCTTTTAAAGAGTGATCGTCAGGATATATTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCTTGCAAGAAGAGTATTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCAGCCATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAAAGAAAGAATAAGAACAGTGCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATAAAGATGCTGCGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTTGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCTTCCATATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTATCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1150

Amino Acids

130.69

Weight (kDa)

5.35

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 274 - 671 2e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
AasI GACNNNNNNGTC 2 cut(s) 1582, 2922
Acc36I ACCTGC 2 cut(s) 1222, 1321
AccB7I CCANNNNNTGG 1 cut(s) 1348
AccBSI CCGCTC 1 cut(s) 1358
AccII CGCG 1 cut(s) 302
AclWI GGATC 2 cut(s) 1256, 2635
AcoI YGGCCR 2 cut(s) 1350, 3019
AcsI RAATTY 6 cut(s) 505, 1661, 2659, 2879, 3267, 3423
AcuI CTGAAG 2 cut(s) 1731, 1872
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 9 cut(s) 183, 203, 620, 1181, 1618, 1653, 1943, 2727, 3447
AflII CTTAAG 1 cut(s) 2125
AhdI GACNNNNNGTC 2 cut(s) 16, 316
AjnI CCWGG 5 cut(s) 1148, 1194, 1781, 2070, 3260
AjuI GAANNNNNNNTTGG 8 cut(s) 77, 109, 860, 892, 1317, 1349, 1470, 1502
AloI GAACNNNNNNTCC 2 cut(s) 2802, 2834
Alw21I GWGCWC 6 cut(s) 789, 934, 1443, 1961, 2356, 2680
Alw26I GTCTC 8 cut(s) 64, 243, 303, 368, 449, 669, 1035, 2908
AlwI GGATC 2 cut(s) 1256, 2635
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 2 cut(s) 769, 825
AoxI GGCC 7 cut(s) 14, 185, 363, 432, 1350, 1968, 3019
ApeKI GCWGC 8 cut(s) 482, 752, 916, 1208, 2282, 2753, 2771, 2908
ApoI RAATTY 6 cut(s) 505, 1661, 2659, 2879, 3267, 3423
ArsI GACNNNNNNTTYG 2 cut(s) 3347, 3379
AseI ATTAAT 1 cut(s) 1058
Asp700I GAANNNNTTC 1 cut(s) 2490
AspLEI GCGC 4 cut(s) 302, 304, 654, 1484
AspS9I GGNCC 7 cut(s) 186, 228, 363, 1494, 1969, 2178, 2508
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 4 cut(s) 88, 710, 752, 1772
AsuNHI GCTAGC 1 cut(s) 2551
AvaI CYCGRG 2 cut(s) 769, 825
AvaII GGWCC 4 cut(s) 228, 1494, 2178, 2508
BalI TGGCCA 2 cut(s) 1352, 3021
BanII GRGCYC 2 cut(s) 1961, 3137
BarI GAAGNNNNNNTAC 2 cut(s) 1759, 1791
BbsI GAAGAC 2 cut(s) 1010, 2021
Bbv12I GWGCWC 6 cut(s) 789, 934, 1443, 1961, 2356, 2680
BbvCI CCTCAGC 1 cut(s) 2541
BbvI GCAGC 8 cut(s) 469, 739, 928, 1195, 2269, 2740, 2758, 2895
BceAI ACGGC 7 cut(s) 7, 110, 184, 200, 328, 370, 2334
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 3131, 3165
BciT130I CCWGG 5 cut(s) 1150, 1196, 1783, 2072, 3262
BciVI GTATCC 1 cut(s) 679
BclI TGATCA 2 cut(s) 1822, 2743
BcnI CCSGG 1 cut(s) 595
BcoDI GTCTC 8 cut(s) 64, 243, 303, 368, 449, 669, 1035, 2908
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 2 cut(s) 655, 1485
BfrI CTTAAG 1 cut(s) 2125
BfuAI ACCTGC 2 cut(s) 1222, 1321
BfuI GTATCC 1 cut(s) 679
BglII AGATCT 1 cut(s) 3367
BisI GCNGC 9 cut(s) 45, 483, 753, 917, 1209, 2283, 2754, 2772, 2909
BlsI GCNGC 9 cut(s) 46, 484, 754, 918, 1210, 2284, 2755, 2773, 2910
BmcAI AGTACT 1 cut(s) 3447
Bme1390I CCNGG 6 cut(s) 595, 1150, 1196, 1783, 2072, 3262
Bme18I GGWCC 4 cut(s) 228, 1494, 2178, 2508
BmeRI GACNNNNNGTC 2 cut(s) 16, 316
BmeT110I CYCGRG 2 cut(s) 769, 825
BmgT120I GGNCC 7 cut(s) 186, 228, 363, 1494, 1969, 2178, 2508
BmiI GGNNCC 3 cut(s) 188, 229, 2179
BmrFI CCNGG 6 cut(s) 595, 1150, 1196, 1783, 2072, 3262
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 7 cut(s) 547, 559, 862, 1716, 1821, 2895, 3203
BmtI GCTAGC 1 cut(s) 2555
BmuI ACTGGG 1 cut(s) 29
BoxI GACNNNNGTC 2 cut(s) 385, 3069
BpiI GAAGAC 2 cut(s) 1010, 2021
BplI GAGNNNNNCTC 2 cut(s) 752, 784
BpmI CTGGAG 4 cut(s) 762, 1132, 1242, 2093
Bpu10I CCTNAGC 4 cut(s) 147, 2066, 2541, 2614
BpuEI CTTGAG 1 cut(s) 1676
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 2 cut(s) 2261, 2729
BsaBI GATNNNNATC 1 cut(s) 1821
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 5 cut(s) 594, 1240, 2194, 2683, 3296
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 5 cut(s) 35, 348, 411, 779, 1762
Bse3DI GCAATG 5 cut(s) 459, 2078, 2601, 2844, 3219
Bse8I GATNNNNATC 1 cut(s) 1821
BseBI CCWGG 5 cut(s) 1150, 1196, 1783, 2072, 3262
BseDI CCNNGG 5 cut(s) 594, 1240, 2194, 2683, 3296
BseJI GATNNNNATC 1 cut(s) 1821
BseMI GCAATG 5 cut(s) 459, 2078, 2601, 2844, 3219
BseNI ACTGG 5 cut(s) 35, 348, 411, 779, 1762
BsePI GCGCGC 1 cut(s) 300
BseRI GAGGAG 5 cut(s) 182, 295, 1397, 1559, 2508
BseXI GCAGC 8 cut(s) 469, 739, 928, 1195, 2269, 2740, 2758, 2895
BseYI CCCAGC 2 cut(s) 189, 2946
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 7 cut(s) 16, 187, 365, 434, 1352, 1970, 3021
BsiHKAI GWGCWC 6 cut(s) 789, 934, 1443, 1961, 2356, 2680
BsiHKCI CYCGRG 2 cut(s) 769, 825
BsiSI CCGG 3 cut(s) 164, 327, 594
BsiWI CGTACG 2 cut(s) 181, 201
BslFI GGGAC 3 cut(s) 53, 1891, 2191
BsmAI GTCTC 8 cut(s) 64, 243, 303, 368, 449, 669, 1035, 2908
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 53, 1891, 2191
BsmI GAATGC 2 cut(s) 1090, 1224
BsnI GGCC 7 cut(s) 16, 187, 365, 434, 1352, 1970, 3021
Bso31I GGTCTC 1 cut(s) 669
BsoBI CYCGRG 2 cut(s) 769, 825
Bsp1286I GDGCHC 7 cut(s) 789, 934, 1443, 1961, 2356, 2680, 3137
Bsp19I CCATGG 2 cut(s) 2194, 3296
BspANI GGCC 7 cut(s) 16, 187, 365, 434, 1352, 1970, 3021
BspFNI CGCG 1 cut(s) 302
BspHI TCATGA 2 cut(s) 859, 2116
BspLI GGNNCC 3 cut(s) 188, 229, 2179
BspMI ACCTGC 2 cut(s) 1222, 1321
BspOI GCTAGC 1 cut(s) 2555
BspPI GGATC 2 cut(s) 1256, 2635
BspQI GCTCTTC 1 cut(s) 2292
BspTI CTTAAG 1 cut(s) 2125
BspTNI GGTCTC 1 cut(s) 669
BsrBI CCGCTC 1 cut(s) 1358
BsrDI GCAATG 5 cut(s) 459, 2078, 2601, 2844, 3219
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 5 cut(s) 35, 348, 411, 779, 1762
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 5 cut(s) 594, 1240, 2194, 2683, 3296
BssHII GCGCGC 1 cut(s) 300
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 4 cut(s) 1240, 2194, 2683, 3296
Bst2UI CCWGG 5 cut(s) 1150, 1196, 1783, 2072, 3262
Bst4CI ACNGT 9 cut(s) 691, 1008, 1046, 1454, 1992, 2042, 2710, 3071, 3450
Bst6I CTCTTC 7 cut(s) 272, 284, 2292, 2413, 2449, 2526, 2778
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 2125
BstBAI YACGTR 2 cut(s) 2261, 2729
BstC8I GCNNGC 9 cut(s) 238, 302, 934, 938, 1213, 2553, 2680, 3244, 3292
BstDSI CCRYGG 2 cut(s) 2194, 3296
BstFNI CGCG 1 cut(s) 302
BstH2I RGCGCY 2 cut(s) 655, 1485
BstHHI GCGC 4 cut(s) 302, 304, 654, 1484
BstMAI GTCTC 8 cut(s) 64, 243, 303, 368, 449, 669, 1035, 2908
BstNI CCWGG 5 cut(s) 1150, 1196, 1783, 2072, 3262
BstNSI RCATGY 4 cut(s) 1130, 1190, 1215, 3246
BstPAI GACNNNNGTC 2 cut(s) 385, 3069
BstSCI CCNGG 6 cut(s) 593, 1148, 1194, 1781, 2070, 3260
BstSFI CTRYAG 1 cut(s) 448
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 8 cut(s) 469, 739, 928, 1195, 2269, 2740, 2758, 2895
BstV2I GAAGAC 2 cut(s) 1010, 2021
BstX2I RGATCY 2 cut(s) 1261, 3367
BstXI CCANNNNNNTGG 1 cut(s) 1504
BstYI RGATCY 2 cut(s) 1261, 3367
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 7 cut(s) 16, 187, 365, 434, 1352, 1970, 3021
BtgI CCRYGG 2 cut(s) 2194, 3296
BtgZI GCGATG 2 cut(s) 255, 3226
BtsIMutI CAGTG 6 cut(s) 355, 418, 1410, 1459, 2030, 2715
BveI ACCTGC 2 cut(s) 1222, 1321
Cac8I GCNNGC 9 cut(s) 238, 302, 934, 938, 1213, 2553, 2680, 3244, 3292
CaiI CAGNNNCTG 1 cut(s) 946
CciI TCATGA 2 cut(s) 859, 2116
CfoI GCGC 4 cut(s) 302, 304, 654, 1484
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 7 cut(s) 186, 228, 363, 1494, 1969, 2178, 2508
CseI GACGC 2 cut(s) 131, 1781
Csp6I GTAC 9 cut(s) 182, 202, 619, 1180, 1617, 1652, 1942, 2726, 3446
CviQI GTAC 9 cut(s) 182, 202, 619, 1180, 1617, 1652, 1942, 2726, 3446
DraI TTTAAA 3 cut(s) 511, 2373, 2644
DrdI GACNNNNNNGTC 2 cut(s) 1582, 2922
DriI GACNNNNNGTC 2 cut(s) 16, 316
DseDI GACNNNNNNGTC 2 cut(s) 1582, 2922
EaeI YGGCCR 2 cut(s) 1350, 3019
Eam1104I CTCTTC 7 cut(s) 272, 284, 2292, 2413, 2449, 2526, 2778
Eam1105I GACNNNNNGTC 2 cut(s) 16, 316
EarI CTCTTC 7 cut(s) 272, 284, 2292, 2413, 2449, 2526, 2778
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 1959
Eco130I CCWWGG 4 cut(s) 1240, 2194, 2683, 3296
Eco24I GRGCYC 2 cut(s) 1961, 3137
Eco31I GGTCTC 1 cut(s) 669
Eco32I GATATC 1 cut(s) 2031
Eco47I GGWCC 4 cut(s) 228, 1494, 2178, 2508
Eco53kI GAGCTC 1 cut(s) 1959
Eco57I CTGAAG 2 cut(s) 1731, 1872
Eco88I CYCGRG 2 cut(s) 769, 825
EcoICRI GAGCTC 1 cut(s) 1959
EcoRII CCWGG 5 cut(s) 1148, 1194, 1781, 2070, 3260
EcoRV GATATC 1 cut(s) 2031
EcoT14I CCWWGG 4 cut(s) 1240, 2194, 2683, 3296
EcoT22I ATGCAT 2 cut(s) 574, 2601
EcoT38I GRGCYC 2 cut(s) 1961, 3137
ErhI CCWWGG 4 cut(s) 1240, 2194, 2683, 3296
Esp3I CGTCTC 1 cut(s) 303
FalI AAGNNNNNCTT 6 cut(s) 1470, 1502, 1890, 1922, 2410, 2442
FaqI GGGAC 3 cut(s) 53, 1891, 2191
FauI CCCGC 6 cut(s) 218, 308, 1273, 1868, 2336, 3378
FauNDI CATATG 1 cut(s) 3226
FbaI TGATCA 2 cut(s) 1822, 2743
Fnu4HI GCNGC 9 cut(s) 45, 483, 753, 917, 1209, 2283, 2754, 2772, 2909
FriOI GRGCYC 2 cut(s) 1961, 3137
Fsp4HI GCNGC 9 cut(s) 45, 483, 753, 917, 1209, 2283, 2754, 2772, 2909
GlaI GCGC 4 cut(s) 301, 303, 653, 1483
GluI GCNGC 9 cut(s) 45, 483, 753, 917, 1209, 2283, 2754, 2772, 2909
GsaI CCCAGC 2 cut(s) 193, 2950
GsuI CTGGAG 4 cut(s) 762, 1132, 1242, 2093
HaeII RGCGCY 2 cut(s) 655, 1485
HaeIII GGCC 7 cut(s) 16, 187, 365, 434, 1352, 1970, 3021
HapII CCGG 3 cut(s) 164, 327, 594
HgaI GACGC 2 cut(s) 131, 1781
HhaI GCGC 4 cut(s) 302, 304, 654, 1484
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 4 cut(s) 300, 302, 652, 1482
HinP1I GCGC 4 cut(s) 300, 302, 652, 1482
HincII GTYRAC 1 cut(s) 2790
HindII GTYRAC 1 cut(s) 2790
HindIII AAGCTT 2 cut(s) 513, 1713
HpaI GTTAAC 1 cut(s) 2790
HpaII CCGG 3 cut(s) 164, 327, 594
HphI GGTGA 4 cut(s) 88, 710, 752, 1772
Hpy166II GTNNAC 4 cut(s) 584, 662, 1497, 2790
Hpy8I GTNNAC 4 cut(s) 584, 662, 1497, 2790
Hpy99I CGWCG 6 cut(s) 11, 22, 28, 125, 182, 391
HpyCH4III ACNGT 9 cut(s) 691, 1008, 1046, 1454, 1992, 2042, 2710, 3071, 3450
HpyCH4IV ACGT 5 cut(s) 66, 180, 2260, 2728, 3035
HpySE526I ACGT 5 cut(s) 66, 180, 2260, 2728, 3035
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 4 cut(s) 300, 302, 652, 1482
Ksp22I TGATCA 2 cut(s) 1822, 2743
KspAI GTTAAC 1 cut(s) 2790
LguI GCTCTTC 1 cut(s) 2292
LmnI GCTCC 6 cut(s) 169, 297, 850, 1410, 2521, 2548
Lsp1109I GCAGC 8 cut(s) 469, 739, 928, 1195, 2269, 2740, 2758, 2895
LweI GCATC 7 cut(s) 547, 559, 862, 1716, 1821, 2895, 3203
MaeII ACGT 5 cut(s) 66, 180, 2260, 2728, 3035
MaeIII GTNAC 3 cut(s) 762, 1986, 2338
MbiI CCGCTC 1 cut(s) 1358
MfeI CAATTG 1 cut(s) 2666
MflI RGATCY 2 cut(s) 1261, 3367
MhlI GDGCHC 7 cut(s) 789, 934, 1443, 1961, 2356, 2680, 3137
MlsI TGGCCA 2 cut(s) 1352, 3021
MluNI TGGCCA 2 cut(s) 1352, 3021
MlyI GAGTC 5 cut(s) 770, 817, 937, 1923, 2960
MmeI TCCRAC 5 cut(s) 108, 369, 1450, 1485, 1553
Mox20I TGGCCA 2 cut(s) 1352, 3021
Mph1103I ATGCAT 2 cut(s) 574, 2601
MroXI GAANNNNTTC 1 cut(s) 2490
MscI TGGCCA 2 cut(s) 1352, 3021
MseI TTAA 9 cut(s) 510, 647, 834, 987, 1058, 2126, 2372, 2643, 2789
MslI CAYNNNNRTG 4 cut(s) 858, 1560, 2023, 2903
Msp20I TGGCCA 2 cut(s) 1352, 3021
MspA1I CMGCKG 2 cut(s) 1208, 2753
MspCI CTTAAG 1 cut(s) 2125
MspI CCGG 3 cut(s) 164, 327, 594
MspR9I CCNGG 6 cut(s) 595, 1150, 1196, 1783, 2072, 3262
MunI CAATTG 1 cut(s) 2666
Mva1269I GAATGC 2 cut(s) 1090, 1224
MvaI CCWGG 5 cut(s) 1150, 1196, 1783, 2072, 3262
MvnI CGCG 1 cut(s) 302
NciI CCSGG 1 cut(s) 595
NcoI CCATGG 2 cut(s) 2194, 3296
NdeI CATATG 1 cut(s) 3226
NheI GCTAGC 1 cut(s) 2551
NlaIV GGNNCC 3 cut(s) 188, 229, 2179
NmuCI GTSAC 1 cut(s) 762
NsiI ATGCAT 2 cut(s) 574, 2601
NspI RCATGY 4 cut(s) 1130, 1190, 1215, 3246
PaeI GCATGC 2 cut(s) 1215, 3246
PagI TCATGA 2 cut(s) 859, 2116
PauI GCGCGC 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 2292
PctI GAATGC 2 cut(s) 1090, 1224
PdmI GAANNNNTTC 1 cut(s) 2490
Pfl23II CGTACG 2 cut(s) 181, 201
PflMI CCANNNNNTGG 1 cut(s) 1348
PkrI GCNGC 9 cut(s) 46, 484, 754, 918, 1210, 2284, 2755, 2773, 2910
PleI GAGTC 5 cut(s) 769, 817, 937, 1922, 2959
PpsI GAGTC 5 cut(s) 769, 817, 937, 1922, 2959
Ppu21I YACGTR 2 cut(s) 2261, 2729
PshAI GACNNNNGTC 2 cut(s) 385, 3069
PshBI ATTAAT 1 cut(s) 1058
PsiI TTATAA 1 cut(s) 1032
Psp124BI GAGCTC 1 cut(s) 1961
Psp6I CCWGG 5 cut(s) 1148, 1194, 1781, 2070, 3260
PspFI CCCAGC 2 cut(s) 189, 2946
PspGI CCWGG 5 cut(s) 1148, 1194, 1781, 2070, 3260
PspLI CGTACG 2 cut(s) 181, 201
PspN4I GGNNCC 3 cut(s) 188, 229, 2179
PspPI GGNCC 7 cut(s) 186, 228, 363, 1494, 1969, 2178, 2508
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 1261, 3367
PteI GCGCGC 1 cut(s) 300
RsaI GTAC 9 cut(s) 183, 203, 620, 1181, 1618, 1653, 1943, 2727, 3447
RsaNI GTAC 9 cut(s) 182, 202, 619, 1180, 1617, 1652, 1942, 2726, 3446
RseI CAYNNNNRTG 4 cut(s) 858, 1560, 2023, 2903
SacI GAGCTC 1 cut(s) 1961
SapI GCTCTTC 1 cut(s) 2292
SaqAI TTAA 9 cut(s) 510, 647, 834, 987, 1058, 2126, 2372, 2643, 2789
SatI GCNGC 9 cut(s) 45, 483, 753, 917, 1209, 2283, 2754, 2772, 2909
Sau96I GGNCC 7 cut(s) 186, 228, 363, 1494, 1969, 2178, 2508
ScaI AGTACT 1 cut(s) 3447
SchI GAGTC 5 cut(s) 770, 817, 937, 1923, 2960
ScrFI CCNGG 6 cut(s) 595, 1150, 1196, 1783, 2072, 3262
SduI GDGCHC 7 cut(s) 789, 934, 1443, 1961, 2356, 2680, 3137
SfaNI GCATC 7 cut(s) 547, 559, 862, 1716, 1821, 2895, 3203
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 4 cut(s) 228, 1494, 2178, 2508
SmiMI CAYNNNNRTG 4 cut(s) 858, 1560, 2023, 2903
SmlI CTYRAG 2 cut(s) 1655, 2125
SmoI CTYRAG 2 cut(s) 1655, 2125
SphI GCATGC 2 cut(s) 1215, 3246
SspI AATATT 1 cut(s) 2440
SstI GAGCTC 1 cut(s) 1961
StyD4I CCNGG 6 cut(s) 593, 1148, 1194, 1781, 2070, 3260
StyI CCWWGG 4 cut(s) 1240, 2194, 2683, 3296
TaaI ACNGT 9 cut(s) 691, 1008, 1046, 1454, 1992, 2042, 2710, 3071, 3450
TaiI ACGT 5 cut(s) 69, 183, 2263, 2731, 3038
TaqI TCGA 4 cut(s) 338, 466, 789, 1910
TaqII GACCGA 1 cut(s) 2562
TatI WGTACW 3 cut(s) 618, 1651, 3445
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 9 cut(s) 510, 647, 834, 987, 1058, 2126, 2372, 2643, 2789
Tru9I TTAA 9 cut(s) 510, 647, 834, 987, 1058, 2126, 2372, 2643, 2789
TscAI CASTG 6 cut(s) 355, 418, 1417, 1459, 2030, 2715
TseFI GTSAC 1 cut(s) 762
TseI GCWGC 8 cut(s) 482, 752, 916, 1208, 2282, 2753, 2771, 2908
Tsp45I GTSAC 1 cut(s) 762
TspGWI ACGGA 3 cut(s) 100, 731, 2628
TspRI CASTG 6 cut(s) 355, 418, 1417, 1459, 2030, 2715
Van91I CCANNNNNTGG 1 cut(s) 1348
Vha464I CTTAAG 1 cut(s) 2125
VpaK11BI GGWCC 4 cut(s) 228, 1494, 2178, 2508
VspI ATTAAT 1 cut(s) 1058
XapI RAATTY 6 cut(s) 505, 1661, 2659, 2879, 3267, 3423
XbaI TCTAGA 1 cut(s) 2821
XceI RCATGY 4 cut(s) 1130, 1190, 1215, 3246
XmnI GAANNNNTTC 1 cut(s) 2490
ZrmI AGTACT 1 cut(s) 3447
Zsp2I ATGCAT 2 cut(s) 574, 2601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.