RchiOBHm_Chr7g0177661

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010115
Physical Location & Seq
Reverse (-)
528000 .. 535175
7176 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15827

Sequence Viewer

Length: 3501 bp
ATGGACGACGACAGGCCGTCGGAAGAAGAAGCGAAGCCGTCGCCATCGCCGTCGCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACCTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCACCGCCACCGCCACTGCCGGAGGAGCAAGACGACGTACGGCCCCAGCCAAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGAGAGCGATGAGAGAGACAGGACGAGCAAGAAGAGGAGGAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGCAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCTTGGGCCGAATCCGAGACTAGACCGTCGGAAAATTATTACTTGGATTCCAGTGGCGACCCTGATAATTTGGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGCTCCGGGAATTTTAAAGCTTTGTATCAGGGGAATCAGACAATTTCAACATTGGATAGAGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCCGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCCAGAGACCTTGCGGATACAGTTTCAGGTGATTTTATTCCTTTGACGGATATTCAAACATCCGATGAAGATGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACAAGGGAGCATCCTCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAGCATTCAGGAAATTACCACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTCAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTACTTGAGGAATTTCAAGAAGAAACTGAGAACGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGTTGGGGCTACTGGTGAGGGTAAAGACGCTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGCGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAGTACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTGGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTCGTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTGTGTTGGGGACCACCAATGACATCCATGGAAAGGCAGACCTGATGAAGTTTCTTTGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTGAATCTGAATCCATCCCGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGTATCTTTTAAAGAGTGATCGTCAGGATATATTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCAAGAAGAGTATTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCAGCCATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAAAGAAAGAATAAGAACAGTGCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCTGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATCAAGATGCTGTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTCGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCTTCCATATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTCTCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTGATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1166

Amino Acids

132.64

Weight (kDa)

5.38

Isoelectric Point (pI)

53.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 290 - 687 2.1e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1080
AasI GACNNNNNNGTC 3 cut(s) 433, 1630, 2970
Acc36I ACCTGC 2 cut(s) 1270, 1369
AccB7I CCANNNNNTGG 1 cut(s) 1396
AccBSI CCGCTC 1 cut(s) 1406
AccII CGCG 1 cut(s) 353
AclWI GGATC 4 cut(s) 261, 274, 1304, 2683
AcoI YGGCCR 2 cut(s) 1398, 3067
AcsI RAATTY 7 cut(s) 553, 1427, 1709, 2707, 2927, 3315, 3471
AcuI CTGAAG 2 cut(s) 1779, 1920
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 9 cut(s) 222, 242, 668, 1229, 1666, 1701, 1991, 2775, 3495
AhdI GACNNNNNGTC 2 cut(s) 16, 367
AjnI CCWGG 5 cut(s) 1196, 1242, 1829, 2118, 3308
AjuI GAANNNNNNNTTGG 8 cut(s) 104, 136, 908, 940, 1365, 1397, 1518, 1550
AloI GAACNNNNNNTCC 2 cut(s) 2850, 2882
Alw21I GWGCWC 5 cut(s) 982, 1491, 2009, 2404, 2728
Alw26I GTCTC 8 cut(s) 91, 282, 354, 419, 497, 717, 1083, 2956
AlwI GGATC 4 cut(s) 261, 274, 1304, 2683
AlwNI CAGNNNCTG 1 cut(s) 994
Ama87I CYCGRG 2 cut(s) 817, 873
AoxI GGCC 7 cut(s) 14, 224, 414, 480, 1398, 2016, 3067
ApeKI GCWGC 7 cut(s) 530, 800, 964, 1256, 2330, 2801, 2819
ApoI RAATTY 7 cut(s) 553, 1427, 1709, 2707, 2927, 3315, 3471
ArsI GACNNNNNNTTYG 2 cut(s) 3395, 3427
AseI ATTAAT 1 cut(s) 1106
Asp700I GAANNNNTTC 1 cut(s) 2538
AspLEI GCGC 4 cut(s) 353, 355, 702, 1532
AspS9I GGNCC 6 cut(s) 225, 414, 1542, 2017, 2226, 2556
AsuC2I CCSGG 2 cut(s) 550, 643
AsuHPI GGTGA 3 cut(s) 115, 758, 1820
AsuNHI GCTAGC 1 cut(s) 2599
AvaI CYCGRG 2 cut(s) 817, 873
AvaII GGWCC 3 cut(s) 1542, 2226, 2556
BalI TGGCCA 2 cut(s) 1400, 3069
BamHI GGATCC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 2009, 3185
BarI GAAGNNNNNNTAC 2 cut(s) 1807, 1839
BbsI GAAGAC 2 cut(s) 1058, 2069
Bbv12I GWGCWC 5 cut(s) 982, 1491, 2009, 2404, 2728
BbvCI CCTCAGC 1 cut(s) 2589
BbvI GCAGC 7 cut(s) 517, 787, 976, 1243, 2317, 2788, 2806
BceAI ACGGC 7 cut(s) 22, 34, 137, 223, 239, 379, 2382
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 3179, 3213
BciT130I CCWGG 5 cut(s) 1198, 1244, 1831, 2120, 3310
BciVI GTATCC 1 cut(s) 727
BclI TGATCA 3 cut(s) 1870, 2491, 2791
BcnI CCSGG 2 cut(s) 550, 643
BcoDI GTCTC 8 cut(s) 91, 282, 354, 419, 497, 717, 1083, 2956
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 2 cut(s) 703, 1533
BfuAI ACCTGC 2 cut(s) 1270, 1369
BfuI GTATCC 1 cut(s) 727
BglII AGATCT 1 cut(s) 3415
BisI GCNGC 8 cut(s) 72, 531, 801, 965, 1257, 2331, 2802, 2820
BlsI GCNGC 8 cut(s) 73, 532, 802, 966, 1258, 2332, 2803, 2821
BmcAI AGTACT 1 cut(s) 3495
Bme1390I CCNGG 7 cut(s) 550, 643, 1198, 1244, 1831, 2120, 3310
Bme18I GGWCC 3 cut(s) 1542, 2226, 2556
BmeRI GACNNNNNGTC 2 cut(s) 16, 367
BmeT110I CYCGRG 2 cut(s) 817, 873
BmgT120I GGNCC 6 cut(s) 225, 414, 1542, 2017, 2226, 2556
BmiI GGNNCC 3 cut(s) 227, 268, 2227
BmrFI CCNGG 7 cut(s) 550, 643, 1198, 1244, 1831, 2120, 3310
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 8 cut(s) 595, 607, 910, 1764, 1869, 2943, 3251, 3420
BmtI GCTAGC 1 cut(s) 2603
BmuI ACTGGG 1 cut(s) 56
BoxI GACNNNNGTC 1 cut(s) 3117
BpiI GAAGAC 2 cut(s) 1058, 2069
BplI GAGNNNNNCTC 2 cut(s) 800, 832
BpmI CTGGAG 4 cut(s) 810, 1180, 1290, 2141
Bpu10I CCTNAGC 4 cut(s) 174, 2114, 2589, 2662
BpuEI CTTGAG 1 cut(s) 1724
BpuMI CCSGG 2 cut(s) 550, 643
BsaAI YACGTR 2 cut(s) 2309, 2777
BsaBI GATNNNNATC 1 cut(s) 1869
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 717
BsaJI CCNNGG 5 cut(s) 642, 1288, 2242, 2731, 3344
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 5 cut(s) 62, 399, 459, 827, 1810
Bse3DI GCAATG 5 cut(s) 507, 2126, 2649, 2892, 3267
Bse8I GATNNNNATC 1 cut(s) 1869
BseBI CCWGG 5 cut(s) 1198, 1244, 1831, 2120, 3310
BseDI CCNNGG 5 cut(s) 642, 1288, 2242, 2731, 3344
BseJI GATNNNNATC 1 cut(s) 1869
BseMI GCAATG 5 cut(s) 507, 2126, 2649, 2892, 3267
BseNI ACTGG 5 cut(s) 62, 399, 459, 827, 1810
BsePI GCGCGC 1 cut(s) 351
BseRI GAGGAG 6 cut(s) 221, 322, 346, 1445, 1607, 2556
BseXI GCAGC 7 cut(s) 517, 787, 976, 1243, 2317, 2788, 2806
BseYI CCCAGC 2 cut(s) 228, 2994
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 7 cut(s) 16, 226, 416, 482, 1400, 2018, 3069
BsiHKAI GWGCWC 5 cut(s) 982, 1491, 2009, 2404, 2728
BsiHKCI CYCGRG 2 cut(s) 817, 873
BsiSI CCGG 4 cut(s) 203, 378, 549, 642
BsiWI CGTACG 2 cut(s) 220, 240
BslFI GGGAC 3 cut(s) 80, 1939, 2239
BsmAI GTCTC 8 cut(s) 91, 282, 354, 419, 497, 717, 1083, 2956
BsmBI CGTCTC 1 cut(s) 354
BsmFI GGGAC 3 cut(s) 80, 1939, 2239
BsmI GAATGC 2 cut(s) 1138, 1272
BsnI GGCC 7 cut(s) 16, 226, 416, 482, 1400, 2018, 3069
Bso31I GGTCTC 1 cut(s) 717
BsoBI CYCGRG 2 cut(s) 817, 873
Bsp1286I GDGCHC 6 cut(s) 982, 1491, 2009, 2404, 2728, 3185
Bsp19I CCATGG 2 cut(s) 2242, 3344
BspANI GGCC 7 cut(s) 16, 226, 416, 482, 1400, 2018, 3069
BspFNI CGCG 1 cut(s) 353
BspHI TCATGA 2 cut(s) 907, 2164
BspLI GGNNCC 3 cut(s) 227, 268, 2227
BspMI ACCTGC 2 cut(s) 1270, 1369
BspOI GCTAGC 1 cut(s) 2603
BspPI GGATC 4 cut(s) 261, 274, 1304, 2683
BspQI GCTCTTC 1 cut(s) 2340
BspTNI GGTCTC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 1406
BsrDI GCAATG 5 cut(s) 507, 2126, 2649, 2892, 3267
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 5 cut(s) 62, 399, 459, 827, 1810
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 5 cut(s) 642, 1288, 2242, 2731, 3344
BssHII GCGCGC 1 cut(s) 351
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 4 cut(s) 1288, 2242, 2731, 3344
Bst2UI CCWGG 5 cut(s) 1198, 1244, 1831, 2120, 3310
Bst6I CTCTTC 9 cut(s) 267, 299, 323, 335, 2340, 2461, 2497, 2574, 2826
BstACI GRCGYC 1 cut(s) 150
BstBAI YACGTR 2 cut(s) 2309, 2777
BstC8I GCNNGC 8 cut(s) 353, 982, 986, 1261, 2601, 2728, 3292, 3340
BstDSI CCRYGG 2 cut(s) 2242, 3344
BstFNI CGCG 1 cut(s) 353
BstH2I RGCGCY 2 cut(s) 703, 1533
BstHHI GCGC 4 cut(s) 353, 355, 702, 1532
BstMAI GTCTC 8 cut(s) 91, 282, 354, 419, 497, 717, 1083, 2956
BstNI CCWGG 5 cut(s) 1198, 1244, 1831, 2120, 3310
BstNSI RCATGY 4 cut(s) 1178, 1238, 1263, 3294
BstPAI GACNNNNGTC 1 cut(s) 3117
BstSCI CCNGG 7 cut(s) 548, 641, 1196, 1242, 1829, 2118, 3308
BstSFI CTRYAG 1 cut(s) 496
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 7 cut(s) 517, 787, 976, 1243, 2317, 2788, 2806
BstV2I GAAGAC 2 cut(s) 1058, 2069
BstX2I RGATCY 3 cut(s) 266, 1309, 3415
BstXI CCANNNNNNTGG 1 cut(s) 1552
BstYI RGATCY 3 cut(s) 266, 1309, 3415
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 7 cut(s) 16, 226, 416, 482, 1400, 2018, 3069
BtgI CCRYGG 2 cut(s) 2242, 3344
BtgZI GCGATG 3 cut(s) 30, 294, 3274
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 7 cut(s) 197, 406, 466, 1458, 1507, 2078, 2763
BveI ACCTGC 2 cut(s) 1270, 1369
Cac8I GCNNGC 8 cut(s) 353, 982, 986, 1261, 2601, 2728, 3292, 3340
CaiI CAGNNNCTG 1 cut(s) 994
CciI TCATGA 2 cut(s) 907, 2164
CfoI GCGC 4 cut(s) 353, 355, 702, 1532
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 6 cut(s) 225, 414, 1542, 2017, 2226, 2556
CseI GACGC 2 cut(s) 158, 1829
Csp6I GTAC 9 cut(s) 221, 241, 667, 1228, 1665, 1700, 1990, 2774, 3494
CviQI GTAC 9 cut(s) 221, 241, 667, 1228, 1665, 1700, 1990, 2774, 3494
DraI TTTAAA 3 cut(s) 559, 2421, 2692
DrdI GACNNNNNNGTC 3 cut(s) 433, 1630, 2970
DriI GACNNNNNGTC 2 cut(s) 16, 367
DseDI GACNNNNNNGTC 3 cut(s) 433, 1630, 2970
EaeI YGGCCR 2 cut(s) 1398, 3067
Eam1104I CTCTTC 9 cut(s) 267, 299, 323, 335, 2340, 2461, 2497, 2574, 2826
Eam1105I GACNNNNNGTC 2 cut(s) 16, 367
EarI CTCTTC 9 cut(s) 267, 299, 323, 335, 2340, 2461, 2497, 2574, 2826
EciI GGCGGA 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 2007
Eco130I CCWWGG 4 cut(s) 1288, 2242, 2731, 3344
Eco24I GRGCYC 2 cut(s) 2009, 3185
Eco31I GGTCTC 1 cut(s) 717
Eco32I GATATC 1 cut(s) 2079
Eco47I GGWCC 3 cut(s) 1542, 2226, 2556
Eco53kI GAGCTC 1 cut(s) 2007
Eco57I CTGAAG 2 cut(s) 1779, 1920
Eco88I CYCGRG 2 cut(s) 817, 873
EcoICRI GAGCTC 1 cut(s) 2007
EcoRI GAATTC 1 cut(s) 1427
EcoRII CCWGG 5 cut(s) 1196, 1242, 1829, 2118, 3308
EcoRV GATATC 1 cut(s) 2079
EcoT14I CCWWGG 4 cut(s) 1288, 2242, 2731, 3344
EcoT22I ATGCAT 2 cut(s) 622, 2649
EcoT38I GRGCYC 2 cut(s) 2009, 3185
ErhI CCWWGG 4 cut(s) 1288, 2242, 2731, 3344
Esp3I CGTCTC 1 cut(s) 354
FalI AAGNNNNNCTT 6 cut(s) 1518, 1550, 1938, 1970, 2458, 2490
FaqI GGGAC 3 cut(s) 80, 1939, 2239
FauI CCCGC 5 cut(s) 359, 1321, 1916, 2384, 3426
FauNDI CATATG 1 cut(s) 3274
FbaI TGATCA 3 cut(s) 1870, 2491, 2791
Fnu4HI GCNGC 8 cut(s) 72, 531, 801, 965, 1257, 2331, 2802, 2820
FriOI GRGCYC 2 cut(s) 2009, 3185
Fsp4HI GCNGC 8 cut(s) 72, 531, 801, 965, 1257, 2331, 2802, 2820
GlaI GCGC 4 cut(s) 352, 354, 701, 1531
GluI GCNGC 8 cut(s) 72, 531, 801, 965, 1257, 2331, 2802, 2820
GsaI CCCAGC 2 cut(s) 232, 2998
GsuI CTGGAG 4 cut(s) 810, 1180, 1290, 2141
HaeII RGCGCY 2 cut(s) 703, 1533
HaeIII GGCC 7 cut(s) 16, 226, 416, 482, 1400, 2018, 3069
HapII CCGG 4 cut(s) 203, 378, 549, 642
HgaI GACGC 2 cut(s) 158, 1829
HhaI GCGC 4 cut(s) 353, 355, 702, 1532
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 4 cut(s) 351, 353, 700, 1530
HinP1I GCGC 4 cut(s) 351, 353, 700, 1530
HincII GTYRAC 1 cut(s) 2838
HindII GTYRAC 1 cut(s) 2838
HindIII AAGCTT 2 cut(s) 561, 1761
HpaI GTTAAC 1 cut(s) 2838
HpaII CCGG 4 cut(s) 203, 378, 549, 642
HphI GGTGA 3 cut(s) 115, 758, 1820
Hpy166II GTNNAC 4 cut(s) 632, 710, 1545, 2838
Hpy8I GTNNAC 4 cut(s) 632, 710, 1545, 2838
Hpy99I CGWCG 7 cut(s) 11, 22, 43, 55, 152, 221, 439
HpyCH4IV ACGT 5 cut(s) 93, 219, 2308, 2776, 3083
HpySE526I ACGT 5 cut(s) 93, 219, 2308, 2776, 3083
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 4 cut(s) 351, 353, 700, 1530
Ksp22I TGATCA 3 cut(s) 1870, 2491, 2791
KspAI GTTAAC 1 cut(s) 2838
LguI GCTCTTC 1 cut(s) 2340
LmnI GCTCC 7 cut(s) 208, 348, 551, 898, 1458, 2569, 2596
Lsp1109I GCAGC 7 cut(s) 517, 787, 976, 1243, 2317, 2788, 2806
LweI GCATC 8 cut(s) 595, 607, 910, 1764, 1869, 2943, 3251, 3420
MaeII ACGT 5 cut(s) 93, 219, 2308, 2776, 3083
MaeIII GTNAC 3 cut(s) 810, 2034, 2386
MbiI CCGCTC 1 cut(s) 1406
MfeI CAATTG 1 cut(s) 2714
MflI RGATCY 3 cut(s) 266, 1309, 3415
MhlI GDGCHC 6 cut(s) 982, 1491, 2009, 2404, 2728, 3185
MlsI TGGCCA 2 cut(s) 1400, 3069
MluNI TGGCCA 2 cut(s) 1400, 3069
MlyI GAGTC 5 cut(s) 818, 865, 985, 1971, 3008
MmeI TCCRAC 5 cut(s) 135, 417, 1498, 1533, 1601
Mox20I TGGCCA 2 cut(s) 1400, 3069
Mph1103I ATGCAT 2 cut(s) 622, 2649
MroXI GAANNNNTTC 1 cut(s) 2538
MscI TGGCCA 2 cut(s) 1400, 3069
MseI TTAA 8 cut(s) 558, 695, 882, 1035, 1106, 2420, 2691, 2837
MslI CAYNNNNRTG 4 cut(s) 906, 1608, 2071, 2951
Msp20I TGGCCA 2 cut(s) 1400, 3069
MspA1I CMGCKG 1 cut(s) 2801
MspI CCGG 4 cut(s) 203, 378, 549, 642
MspR9I CCNGG 7 cut(s) 550, 643, 1198, 1244, 1831, 2120, 3310
MunI CAATTG 1 cut(s) 2714
Mva1269I GAATGC 2 cut(s) 1138, 1272
MvaI CCWGG 5 cut(s) 1198, 1244, 1831, 2120, 3310
MvnI CGCG 1 cut(s) 353
NciI CCSGG 2 cut(s) 550, 643
NcoI CCATGG 2 cut(s) 2242, 3344
NdeI CATATG 1 cut(s) 3274
NheI GCTAGC 1 cut(s) 2599
NlaIV GGNNCC 3 cut(s) 227, 268, 2227
NmuCI GTSAC 1 cut(s) 810
NsiI ATGCAT 2 cut(s) 622, 2649
NspI RCATGY 4 cut(s) 1178, 1238, 1263, 3294
PaeI GCATGC 2 cut(s) 1263, 3294
PagI TCATGA 2 cut(s) 907, 2164
PauI GCGCGC 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 2340
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PctI GAATGC 2 cut(s) 1138, 1272
PdmI GAANNNNTTC 1 cut(s) 2538
Pfl23II CGTACG 2 cut(s) 220, 240
PflMI CCANNNNNTGG 1 cut(s) 1396
PfoI TCCNGGA 1 cut(s) 548
PkrI GCNGC 8 cut(s) 73, 532, 802, 966, 1258, 2332, 2803, 2821
PleI GAGTC 5 cut(s) 817, 865, 985, 1970, 3007
PpsI GAGTC 5 cut(s) 817, 865, 985, 1970, 3007
Ppu21I YACGTR 2 cut(s) 2309, 2777
PshAI GACNNNNGTC 1 cut(s) 3117
PshBI ATTAAT 1 cut(s) 1106
PsiI TTATAA 1 cut(s) 1080
Psp124BI GAGCTC 1 cut(s) 2009
Psp6I CCWGG 5 cut(s) 1196, 1242, 1829, 2118, 3308
PspFI CCCAGC 2 cut(s) 228, 2994
PspGI CCWGG 5 cut(s) 1196, 1242, 1829, 2118, 3308
PspLI CGTACG 2 cut(s) 220, 240
PspN4I GGNNCC 3 cut(s) 227, 268, 2227
PspPI GGNCC 6 cut(s) 225, 414, 1542, 2017, 2226, 2556
PstNI CAGNNNCTG 1 cut(s) 994
PsuI RGATCY 3 cut(s) 266, 1309, 3415
PteI GCGCGC 1 cut(s) 351
RsaI GTAC 9 cut(s) 222, 242, 668, 1229, 1666, 1701, 1991, 2775, 3495
RsaNI GTAC 9 cut(s) 221, 241, 667, 1228, 1665, 1700, 1990, 2774, 3494
RseI CAYNNNNRTG 4 cut(s) 906, 1608, 2071, 2951
SacI GAGCTC 1 cut(s) 2009
SapI GCTCTTC 1 cut(s) 2340
SaqAI TTAA 8 cut(s) 558, 695, 882, 1035, 1106, 2420, 2691, 2837
SatI GCNGC 8 cut(s) 72, 531, 801, 965, 1257, 2331, 2802, 2820
Sau96I GGNCC 6 cut(s) 225, 414, 1542, 2017, 2226, 2556
ScaI AGTACT 1 cut(s) 3495
SchI GAGTC 5 cut(s) 818, 865, 985, 1971, 3008
ScrFI CCNGG 7 cut(s) 550, 643, 1198, 1244, 1831, 2120, 3310
SduI GDGCHC 6 cut(s) 982, 1491, 2009, 2404, 2728, 3185
SfaNI GCATC 8 cut(s) 595, 607, 910, 1764, 1869, 2943, 3251, 3420
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 3 cut(s) 1542, 2226, 2556
SmiMI CAYNNNNRTG 4 cut(s) 906, 1608, 2071, 2951
SmlI CTYRAG 1 cut(s) 1703
SmoI CTYRAG 1 cut(s) 1703
SphI GCATGC 2 cut(s) 1263, 3294
SspI AATATT 1 cut(s) 2488
SstI GAGCTC 1 cut(s) 2009
StyD4I CCNGG 7 cut(s) 548, 641, 1196, 1242, 1829, 2118, 3308
StyI CCWWGG 4 cut(s) 1288, 2242, 2731, 3344
TaiI ACGT 5 cut(s) 96, 222, 2311, 2779, 3086
TaqI TCGA 4 cut(s) 389, 514, 837, 1958
TaqII GACCGA 1 cut(s) 2610
TatI WGTACW 3 cut(s) 666, 1699, 3493
TauI GCSGC 1 cut(s) 74
Tru1I TTAA 8 cut(s) 558, 695, 882, 1035, 1106, 2420, 2691, 2837
Tru9I TTAA 8 cut(s) 558, 695, 882, 1035, 1106, 2420, 2691, 2837
TscAI CASTG 7 cut(s) 204, 406, 466, 1465, 1507, 2078, 2763
TseFI GTSAC 1 cut(s) 810
TseI GCWGC 7 cut(s) 530, 800, 964, 1256, 2330, 2801, 2819
Tsp45I GTSAC 1 cut(s) 810
TspGWI ACGGA 3 cut(s) 127, 779, 2676
TspRI CASTG 7 cut(s) 204, 406, 466, 1465, 1507, 2078, 2763
Van91I CCANNNNNTGG 1 cut(s) 1396
VpaK11BI GGWCC 3 cut(s) 1542, 2226, 2556
VspI ATTAAT 1 cut(s) 1106
XapI RAATTY 7 cut(s) 553, 1427, 1709, 2707, 2927, 3315, 3471
XbaI TCTAGA 1 cut(s) 2869
XceI RCATGY 4 cut(s) 1178, 1238, 1263, 3294
XmnI GAANNNNTTC 1 cut(s) 2538
ZrmI AGTACT 1 cut(s) 3495
Zsp2I ATGCAT 2 cut(s) 622, 2649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.