Rh7BG007700

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010115
Physical Location & Seq
Reverse (-)
453504 .. 460313
6810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG007700.1

Sequence Viewer

Length: 3483 bp
ATGGACGACGACAGGCCGTCGGAAGAAGAAGCGAAGCCGTCGCCGTCGCCGTCGCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACGTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCGGAGGAGCAAGAGGACGTACGGCCCCAGCCGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGCGAGCGATCAGAGAGACAGGACGAGCAAGAAGAGGAGGAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGGAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCTTGGGTCGAATCCGAGACTAGACCGTCGGAAAATTATTACTTGGATTCCATTGGCGACCTTGATAATTTGGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGTTCCGGGAATTTTAAAGCTTTGTATCAGGGGAATCAGACAATTTCAACATTGGATAGAGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCCGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCCAGAGACCTTGCGGACACAGTTTCAGCTGATTTTATTCCTTTGACGGATATTCAAACATCCGATGAAGGTGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACGAGGGAGCATCCTCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAACATTCTGGAAATTACAACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTCAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGATCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTACTTGAGGAATTTCAAGAAGAAACTGAGAATGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTTGGTATTGATGTTGATGTTGGGGCTACTGGTGAGGGTAAAGACGCTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATTAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGCGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCCGAGTACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCGACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTGGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTCTTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTCTGTTGGGGACCACCAATGACATCCATGGAAAGACAGACCTGATGAAGTTTCTTCGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTGAATCTGAATCCATCCCGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGTATCTTTTAAAGAGTGATCGTCAGGATATATTGCTATGTGGAGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCAAGAAGAGTATTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCAGCAATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAAAGAAAGAATAAGAACAGTTCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATGAAGATGCTGTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTCGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTATGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCTTCCACATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTCTCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1160

Amino Acids

132.14

Weight (kDa)

5.43

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 284 - 681 5.3e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1062
AasI GACNNNNNNGTC 3 cut(s) 415, 1612, 2952
Acc36I ACCTGC 2 cut(s) 1252, 1351
AccB7I CCANNNNNTGG 1 cut(s) 1378
AccBSI CCGCTC 1 cut(s) 1388
AccII CGCG 1 cut(s) 335
AclWI GGATC 4 cut(s) 243, 256, 1286, 2665
AcoI YGGCCR 2 cut(s) 1380, 3049
AcsI RAATTY 7 cut(s) 535, 1409, 1691, 2689, 2909, 3297, 3453
AcuI CTGAAG 2 cut(s) 1761, 1902
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 9 cut(s) 204, 224, 650, 1211, 1648, 1683, 1973, 2757, 3477
AfiI CCNNNNNNNGG 9 cut(s) 366, 387, 931, 956, 1378, 2101, 2386, 3291, 3327
AflII CTTAAG 1 cut(s) 2155
AhdI GACNNNNNGTC 2 cut(s) 16, 349
AjnI CCWGG 5 cut(s) 1178, 1224, 1811, 2100, 3290
AjuI GAANNNNNNNTTGG 8 cut(s) 104, 136, 890, 922, 1347, 1379, 1500, 1532
AloI GAACNNNNNNTCC 2 cut(s) 2832, 2864
Alw21I GWGCWC 4 cut(s) 964, 1991, 2386, 2710
Alw26I GTCTC 9 cut(s) 91, 139, 264, 336, 401, 479, 699, 1065, 2938
AlwI GGATC 4 cut(s) 243, 256, 1286, 2665
AlwNI CAGNNNCTG 1 cut(s) 976
Ama87I CYCGRG 2 cut(s) 799, 855
AoxI GGCC 6 cut(s) 14, 206, 462, 1380, 1998, 3049
ApeKI GCWGC 7 cut(s) 512, 782, 946, 1238, 2312, 2783, 2801
ApoI RAATTY 7 cut(s) 535, 1409, 1691, 2689, 2909, 3297, 3453
ArsI GACNNNNNNTTYG 2 cut(s) 3377, 3409
AseI ATTAAT 1 cut(s) 1088
Asp700I GAANNNNTTC 1 cut(s) 2520
AspLEI GCGC 3 cut(s) 335, 684, 1514
AspS9I GGNCC 5 cut(s) 207, 1524, 1999, 2208, 2538
AsuC2I CCSGG 2 cut(s) 532, 625
AsuHPI GGTGA 3 cut(s) 115, 782, 1802
AsuNHI GCTAGC 1 cut(s) 2581
AvaI CYCGRG 2 cut(s) 799, 855
AvaII GGWCC 3 cut(s) 1524, 2208, 2538
BalI TGGCCA 2 cut(s) 1382, 3051
BamHI GGATCC 1 cut(s) 248
BanII GRGCYC 2 cut(s) 1991, 3167
BarI GAAGNNNNNNTAC 2 cut(s) 1789, 1821
BbsI GAAGAC 2 cut(s) 1040, 2051
Bbv12I GWGCWC 4 cut(s) 964, 1991, 2386, 2710
BbvCI CCTCAGC 1 cut(s) 2571
BbvI GCAGC 7 cut(s) 499, 769, 958, 1225, 2299, 2770, 2788
BceAI ACGGC 8 cut(s) 22, 28, 34, 137, 205, 221, 361, 2364
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 3161, 3195
BciT130I CCWGG 5 cut(s) 1180, 1226, 1813, 2102, 3292
BclI TGATCA 4 cut(s) 1468, 1852, 2473, 2773
BcnI CCSGG 2 cut(s) 532, 625
BcoDI GTCTC 9 cut(s) 91, 139, 264, 336, 401, 479, 699, 1065, 2938
BfmI CTRYAG 1 cut(s) 478
BfoI RGCGCY 2 cut(s) 685, 1515
BfrI CTTAAG 1 cut(s) 2155
BfuAI ACCTGC 2 cut(s) 1252, 1351
BglII AGATCT 1 cut(s) 3397
BisI GCNGC 8 cut(s) 72, 513, 783, 947, 1239, 2313, 2784, 2802
BlsI GCNGC 8 cut(s) 73, 514, 784, 948, 1240, 2314, 2785, 2803
BmcAI AGTACT 1 cut(s) 3477
Bme1390I CCNGG 7 cut(s) 532, 625, 1180, 1226, 1813, 2102, 3292
Bme18I GGWCC 3 cut(s) 1524, 2208, 2538
BmeRI GACNNNNNGTC 2 cut(s) 16, 349
BmeT110I CYCGRG 2 cut(s) 799, 855
BmgT120I GGNCC 5 cut(s) 207, 1524, 1999, 2208, 2538
BmiI GGNNCC 3 cut(s) 209, 250, 2209
BmrFI CCNGG 7 cut(s) 532, 625, 1180, 1226, 1813, 2102, 3292
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 7 cut(s) 577, 589, 892, 1746, 1851, 2925, 3233
BmtI GCTAGC 1 cut(s) 2585
BmuI ACTGGG 1 cut(s) 56
BoxI GACNNNNGTC 1 cut(s) 3099
BpiI GAAGAC 2 cut(s) 1040, 2051
BplI GAGNNNNNCTC 2 cut(s) 782, 814
BpmI CTGGAG 4 cut(s) 792, 1162, 1272, 2123
Bpu10I CCTNAGC 4 cut(s) 174, 2096, 2571, 2644
BpuEI CTTGAG 1 cut(s) 1706
BpuMI CCSGG 2 cut(s) 532, 625
BsaAI YACGTR 2 cut(s) 2291, 2759
BsaBI GATNNNNATC 1 cut(s) 1851
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 5 cut(s) 624, 1270, 2224, 2713, 3326
Bsc4I CCNNNNNNNGG 9 cut(s) 366, 387, 931, 956, 1378, 2101, 2386, 3291, 3327
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 4 cut(s) 62, 381, 809, 1792
Bse3DI GCAATG 5 cut(s) 489, 2108, 2631, 2874, 3249
Bse8I GATNNNNATC 1 cut(s) 1851
BseBI CCWGG 5 cut(s) 1180, 1226, 1813, 2102, 3292
BseDI CCNNGG 5 cut(s) 624, 1270, 2224, 2713, 3326
BseJI GATNNNNATC 1 cut(s) 1851
BseLI CCNNNNNNNGG 9 cut(s) 366, 387, 931, 956, 1378, 2101, 2386, 3291, 3327
BseMI GCAATG 5 cut(s) 489, 2108, 2631, 2874, 3249
BseNI ACTGG 4 cut(s) 62, 381, 809, 1792
BseRI GAGGAG 6 cut(s) 203, 304, 328, 1427, 1589, 2538
BseXI GCAGC 7 cut(s) 499, 769, 958, 1225, 2299, 2770, 2788
BseYI CCCAGC 2 cut(s) 210, 2976
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 6 cut(s) 16, 208, 464, 1382, 2000, 3051
BsiHKAI GWGCWC 4 cut(s) 964, 1991, 2386, 2710
BsiHKCI CYCGRG 2 cut(s) 799, 855
BsiSI CCGG 4 cut(s) 185, 360, 531, 624
BsiWI CGTACG 2 cut(s) 202, 222
BslFI GGGAC 3 cut(s) 80, 1921, 2221
BslI CCNNNNNNNGG 9 cut(s) 366, 387, 931, 956, 1378, 2101, 2386, 3291, 3327
BsmAI GTCTC 9 cut(s) 91, 139, 264, 336, 401, 479, 699, 1065, 2938
BsmBI CGTCTC 2 cut(s) 139, 336
BsmFI GGGAC 3 cut(s) 80, 1921, 2221
BsmI GAATGC 1 cut(s) 1254
BsnI GGCC 6 cut(s) 16, 208, 464, 1382, 2000, 3051
Bso31I GGTCTC 1 cut(s) 699
BsoBI CYCGRG 2 cut(s) 799, 855
Bsp1286I GDGCHC 5 cut(s) 964, 1991, 2386, 2710, 3167
Bsp19I CCATGG 2 cut(s) 2224, 3326
BspANI GGCC 6 cut(s) 16, 208, 464, 1382, 2000, 3051
BspFNI CGCG 1 cut(s) 335
BspHI TCATGA 2 cut(s) 889, 2146
BspLI GGNNCC 3 cut(s) 209, 250, 2209
BspMI ACCTGC 2 cut(s) 1252, 1351
BspOI GCTAGC 1 cut(s) 2585
BspPI GGATC 4 cut(s) 243, 256, 1286, 2665
BspQI GCTCTTC 1 cut(s) 2322
BspTI CTTAAG 1 cut(s) 2155
BspTNI GGTCTC 1 cut(s) 699
BsrBI CCGCTC 1 cut(s) 1388
BsrDI GCAATG 5 cut(s) 489, 2108, 2631, 2874, 3249
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 4 cut(s) 62, 381, 809, 1792
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 5 cut(s) 624, 1270, 2224, 2713, 3326
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 4 cut(s) 1270, 2224, 2713, 3326
Bst2UI CCWGG 5 cut(s) 1180, 1226, 1813, 2102, 3292
Bst6I CTCTTC 8 cut(s) 281, 305, 317, 2322, 2443, 2479, 2556, 2808
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 2155
BstBAI YACGTR 2 cut(s) 2291, 2759
BstC8I GCNNGC 8 cut(s) 259, 964, 968, 1243, 2583, 2710, 3274, 3322
BstDSI CCRYGG 2 cut(s) 2224, 3326
BstFNI CGCG 1 cut(s) 335
BstH2I RGCGCY 2 cut(s) 685, 1515
BstHHI GCGC 3 cut(s) 335, 684, 1514
BstMAI GTCTC 9 cut(s) 91, 139, 264, 336, 401, 479, 699, 1065, 2938
BstNI CCWGG 5 cut(s) 1180, 1226, 1813, 2102, 3292
BstNSI RCATGY 4 cut(s) 1160, 1220, 1245, 3276
BstPAI GACNNNNGTC 1 cut(s) 3099
BstSCI CCNGG 7 cut(s) 530, 623, 1178, 1224, 1811, 2100, 3290
BstSFI CTRYAG 1 cut(s) 478
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 7 cut(s) 499, 769, 958, 1225, 2299, 2770, 2788
BstV2I GAAGAC 2 cut(s) 1040, 2051
BstX2I RGATCY 3 cut(s) 248, 1291, 3397
BstXI CCANNNNNNTGG 1 cut(s) 1534
BstYI RGATCY 3 cut(s) 248, 1291, 3397
BsuRI GGCC 6 cut(s) 16, 208, 464, 1382, 2000, 3051
BtgI CCRYGG 2 cut(s) 2224, 3326
BtgZI GCGATG 1 cut(s) 3256
BtsIMutI CAGTG 4 cut(s) 388, 1440, 1489, 2060
BveI ACCTGC 2 cut(s) 1252, 1351
Cac8I GCNNGC 8 cut(s) 259, 964, 968, 1243, 2583, 2710, 3274, 3322
CaiI CAGNNNCTG 1 cut(s) 976
CciI TCATGA 2 cut(s) 889, 2146
CfoI GCGC 3 cut(s) 335, 684, 1514
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 5 cut(s) 207, 1524, 1999, 2208, 2538
CseI GACGC 2 cut(s) 158, 1811
Csp6I GTAC 9 cut(s) 203, 223, 649, 1210, 1647, 1682, 1972, 2756, 3476
CviQI GTAC 9 cut(s) 203, 223, 649, 1210, 1647, 1682, 1972, 2756, 3476
DraI TTTAAA 3 cut(s) 541, 2403, 2674
DrdI GACNNNNNNGTC 3 cut(s) 415, 1612, 2952
DriI GACNNNNNGTC 2 cut(s) 16, 349
DseDI GACNNNNNNGTC 3 cut(s) 415, 1612, 2952
EaeI YGGCCR 2 cut(s) 1380, 3049
Eam1104I CTCTTC 8 cut(s) 281, 305, 317, 2322, 2443, 2479, 2556, 2808
Eam1105I GACNNNNNGTC 2 cut(s) 16, 349
EarI CTCTTC 8 cut(s) 281, 305, 317, 2322, 2443, 2479, 2556, 2808
EciI GGCGGA 1 cut(s) 343
Ecl136II GAGCTC 1 cut(s) 1989
Eco130I CCWWGG 4 cut(s) 1270, 2224, 2713, 3326
Eco24I GRGCYC 2 cut(s) 1991, 3167
Eco31I GGTCTC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 2061
Eco47I GGWCC 3 cut(s) 1524, 2208, 2538
Eco53kI GAGCTC 1 cut(s) 1989
Eco57I CTGAAG 2 cut(s) 1761, 1902
Eco88I CYCGRG 2 cut(s) 799, 855
EcoICRI GAGCTC 1 cut(s) 1989
EcoRI GAATTC 1 cut(s) 1409
EcoRII CCWGG 5 cut(s) 1178, 1224, 1811, 2100, 3290
EcoRV GATATC 1 cut(s) 2061
EcoT14I CCWWGG 4 cut(s) 1270, 2224, 2713, 3326
EcoT22I ATGCAT 2 cut(s) 604, 2631
EcoT38I GRGCYC 2 cut(s) 1991, 3167
ErhI CCWWGG 4 cut(s) 1270, 2224, 2713, 3326
Esp3I CGTCTC 2 cut(s) 139, 336
FalI AAGNNNNNCTT 6 cut(s) 1500, 1532, 1920, 1952, 2440, 2472
FaqI GGGAC 3 cut(s) 80, 1921, 2221
FauI CCCGC 5 cut(s) 341, 1303, 1898, 2366, 3408
FauNDI CATATG 1 cut(s) 3256
FbaI TGATCA 4 cut(s) 1468, 1852, 2473, 2773
Fnu4HI GCNGC 8 cut(s) 72, 513, 783, 947, 1239, 2313, 2784, 2802
FriOI GRGCYC 2 cut(s) 1991, 3167
Fsp4HI GCNGC 8 cut(s) 72, 513, 783, 947, 1239, 2313, 2784, 2802
GlaI GCGC 3 cut(s) 334, 683, 1513
GluI GCNGC 8 cut(s) 72, 513, 783, 947, 1239, 2313, 2784, 2802
GsaI CCCAGC 2 cut(s) 214, 2980
GsuI CTGGAG 4 cut(s) 792, 1162, 1272, 2123
HaeII RGCGCY 2 cut(s) 685, 1515
HaeIII GGCC 6 cut(s) 16, 208, 464, 1382, 2000, 3051
HapII CCGG 4 cut(s) 185, 360, 531, 624
HgaI GACGC 2 cut(s) 158, 1811
HhaI GCGC 3 cut(s) 335, 684, 1514
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 3 cut(s) 333, 682, 1512
HinP1I GCGC 3 cut(s) 333, 682, 1512
HincII GTYRAC 1 cut(s) 2820
HindII GTYRAC 1 cut(s) 2820
HindIII AAGCTT 2 cut(s) 543, 1743
HpaI GTTAAC 1 cut(s) 2820
HpaII CCGG 4 cut(s) 185, 360, 531, 624
HphI GGTGA 3 cut(s) 115, 782, 1802
Hpy166II GTNNAC 4 cut(s) 614, 692, 1527, 2820
Hpy8I GTNNAC 4 cut(s) 614, 692, 1527, 2820
Hpy99I CGWCG 7 cut(s) 11, 22, 43, 49, 55, 152, 421
HpyCH4IV ACGT 6 cut(s) 93, 132, 201, 2290, 2758, 3065
HpySE526I ACGT 6 cut(s) 93, 132, 201, 2290, 2758, 3065
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 3 cut(s) 333, 682, 1512
Ksp22I TGATCA 4 cut(s) 1468, 1852, 2473, 2773
KspAI GTTAAC 1 cut(s) 2820
LguI GCTCTTC 1 cut(s) 2322
LmnI GCTCC 6 cut(s) 190, 330, 880, 1440, 2551, 2578
Lsp1109I GCAGC 7 cut(s) 499, 769, 958, 1225, 2299, 2770, 2788
LweI GCATC 7 cut(s) 577, 589, 892, 1746, 1851, 2925, 3233
MaeII ACGT 6 cut(s) 93, 132, 201, 2290, 2758, 3065
MaeIII GTNAC 3 cut(s) 792, 2016, 2368
MbiI CCGCTC 1 cut(s) 1388
MfeI CAATTG 1 cut(s) 2696
MflI RGATCY 3 cut(s) 248, 1291, 3397
MhlI GDGCHC 5 cut(s) 964, 1991, 2386, 2710, 3167
MlsI TGGCCA 2 cut(s) 1382, 3051
MluNI TGGCCA 2 cut(s) 1382, 3051
MlyI GAGTC 5 cut(s) 800, 847, 967, 1953, 2990
MmeI TCCRAC 5 cut(s) 135, 399, 1480, 1515, 1583
Mox20I TGGCCA 2 cut(s) 1382, 3051
Mph1103I ATGCAT 2 cut(s) 604, 2631
MroXI GAANNNNTTC 1 cut(s) 2520
MscI TGGCCA 2 cut(s) 1382, 3051
MslI CAYNNNNRTG 4 cut(s) 888, 1590, 2053, 2933
Msp20I TGGCCA 2 cut(s) 1382, 3051
MspA1I CMGCKG 2 cut(s) 728, 2783
MspCI CTTAAG 1 cut(s) 2155
MspI CCGG 4 cut(s) 185, 360, 531, 624
MspR9I CCNGG 7 cut(s) 532, 625, 1180, 1226, 1813, 2102, 3292
MunI CAATTG 1 cut(s) 2696
Mva1269I GAATGC 1 cut(s) 1254
MvaI CCWGG 5 cut(s) 1180, 1226, 1813, 2102, 3292
MvnI CGCG 1 cut(s) 335
NciI CCSGG 2 cut(s) 532, 625
NcoI CCATGG 2 cut(s) 2224, 3326
NdeI CATATG 1 cut(s) 3256
NheI GCTAGC 1 cut(s) 2581
NlaIV GGNNCC 3 cut(s) 209, 250, 2209
NmeAIII GCCGAG 1 cut(s) 1993
NmuCI GTSAC 1 cut(s) 792
NsiI ATGCAT 2 cut(s) 604, 2631
NspI RCATGY 4 cut(s) 1160, 1220, 1245, 3276
PaeI GCATGC 2 cut(s) 1245, 3276
PagI TCATGA 2 cut(s) 889, 2146
PciSI GCTCTTC 1 cut(s) 2322
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PctI GAATGC 1 cut(s) 1254
PdmI GAANNNNTTC 1 cut(s) 2520
Pfl23II CGTACG 2 cut(s) 202, 222
PflMI CCANNNNNTGG 1 cut(s) 1378
PfoI TCCNGGA 1 cut(s) 530
PkrI GCNGC 8 cut(s) 73, 514, 784, 948, 1240, 2314, 2785, 2803
PleI GAGTC 5 cut(s) 799, 847, 967, 1952, 2989
PpsI GAGTC 5 cut(s) 799, 847, 967, 1952, 2989
Ppu21I YACGTR 2 cut(s) 2291, 2759
PshAI GACNNNNGTC 1 cut(s) 3099
PshBI ATTAAT 1 cut(s) 1088
PsiI TTATAA 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 1991
Psp6I CCWGG 5 cut(s) 1178, 1224, 1811, 2100, 3290
PspFI CCCAGC 2 cut(s) 210, 2976
PspGI CCWGG 5 cut(s) 1178, 1224, 1811, 2100, 3290
PspLI CGTACG 2 cut(s) 202, 222
PspN4I GGNNCC 3 cut(s) 209, 250, 2209
PspPI GGNCC 5 cut(s) 207, 1524, 1999, 2208, 2538
PstNI CAGNNNCTG 1 cut(s) 976
PsuI RGATCY 3 cut(s) 248, 1291, 3397
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 9 cut(s) 204, 224, 650, 1211, 1648, 1683, 1973, 2757, 3477
RsaNI GTAC 9 cut(s) 203, 223, 649, 1210, 1647, 1682, 1972, 2756, 3476
RseI CAYNNNNRTG 4 cut(s) 888, 1590, 2053, 2933
SacI GAGCTC 1 cut(s) 1991
SapI GCTCTTC 1 cut(s) 2322
SatI GCNGC 8 cut(s) 72, 513, 783, 947, 1239, 2313, 2784, 2802
Sau96I GGNCC 5 cut(s) 207, 1524, 1999, 2208, 2538
ScaI AGTACT 1 cut(s) 3477
SchI GAGTC 5 cut(s) 800, 847, 967, 1953, 2990
ScrFI CCNGG 7 cut(s) 532, 625, 1180, 1226, 1813, 2102, 3292
SduI GDGCHC 5 cut(s) 964, 1991, 2386, 2710, 3167
SfaNI GCATC 7 cut(s) 577, 589, 892, 1746, 1851, 2925, 3233
SfcI CTRYAG 1 cut(s) 478
SinI GGWCC 3 cut(s) 1524, 2208, 2538
SmiMI CAYNNNNRTG 4 cut(s) 888, 1590, 2053, 2933
SmlI CTYRAG 2 cut(s) 1685, 2155
SmoI CTYRAG 2 cut(s) 1685, 2155
SphI GCATGC 2 cut(s) 1245, 3276
SspI AATATT 1 cut(s) 2470
SstI GAGCTC 1 cut(s) 1991
StyD4I CCNGG 7 cut(s) 530, 623, 1178, 1224, 1811, 2100, 3290
StyI CCWWGG 4 cut(s) 1270, 2224, 2713, 3326
TaiI ACGT 6 cut(s) 96, 135, 204, 2293, 2761, 3068
TaqI TCGA 6 cut(s) 371, 399, 496, 819, 1940, 2063
TaqII GACCGA 1 cut(s) 2592
TatI WGTACW 3 cut(s) 648, 1681, 3475
TauI GCSGC 1 cut(s) 74
TscAI CASTG 4 cut(s) 388, 1447, 1489, 2060
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 7 cut(s) 512, 782, 946, 1238, 2312, 2783, 2801
Tsp45I GTSAC 1 cut(s) 792
TspGWI ACGGA 3 cut(s) 127, 761, 2658
TspRI CASTG 4 cut(s) 388, 1447, 1489, 2060
Van91I CCANNNNNTGG 1 cut(s) 1378
Vha464I CTTAAG 1 cut(s) 2155
VpaK11BI GGWCC 3 cut(s) 1524, 2208, 2538
VspI ATTAAT 1 cut(s) 1088
XapI RAATTY 7 cut(s) 535, 1409, 1691, 2689, 2909, 3297, 3453
XbaI TCTAGA 1 cut(s) 2851
XceI RCATGY 4 cut(s) 1160, 1220, 1245, 3276
XmnI GAANNNNTTC 1 cut(s) 2520
ZrmI AGTACT 1 cut(s) 3477
Zsp2I ATGCAT 2 cut(s) 604, 2631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.