Rh7AG007800

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010115
Physical Location & Seq
Reverse (-)
520640 .. 527390
6751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG007800.1

Sequence Viewer

Length: 3450 bp
ATGGACGACGACAGGCCGTCGCCGTCGCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTCTCCAACACCAGCTTCACCACCAACCTCTCCGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCACCGCCGGAGGAGCAAGACGACGTACGGCCGCGGCCGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGCGAGCGATGAGAGAGACAGGACGAGCAAGAAGGAGAAGGGGAAGAGGAGGAAGAAGAGGCGGAGCGCGCAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCATGGGCCGAATCCGAGACTAGACCGTCGGAAAATTATTACTTGGATTCCAGTGGCGACCTTGATAATTTAGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGCTCCGGGAATTTTAAAGCTTTGTATCAGGGGAATCAGAAAATTTCAACATTGGATAGAGATGCTGATGTTGATGCATTGGATGGTAAACTAAAATCCGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCTAGAGACCTTGCGGATACAGTTTCAGGTGATTTTATTCCTTTGACGGATATTCAAACATCCGATGAAGGTGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACGAGGGAGCATCCTCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGGCTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACATCATTCAGGAAATTACCACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTCAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTACTTGAGGAATTTCAAGAAGAAACTGAGAACGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGTTGGGGCTACTGGTGAGGTTAAAGACGCTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGCGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAGTACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGACATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTAGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTGTTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTCTGTTGGGGACCACCAATGACATTCATGGACAGGCAGACCTGATGAAGTTTCTTTGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTGAATCTGAATCTATCCCGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGCATCTTTTAAAGAGTGATCGTCAGGATATATTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCAAGAAGAGTACTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCGGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTTGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCAGCCATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAAAGAAAGAATAAGAACAGTGCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCTGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCAGGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATGAAGATGCTGCGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTTGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCTTCCATATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTCTCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1149

Amino Acids

130.72

Weight (kDa)

5.46

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 273 - 670 3.5e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
AasI GACNNNNNNGTC 3 cut(s) 382, 1579, 2919
Acc36I ACCTGC 2 cut(s) 1219, 1318
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccBSI CCGCTC 1 cut(s) 1355
AccII CGCG 2 cut(s) 190, 302
AclWI GGATC 4 cut(s) 222, 235, 1253, 2632
AcoI YGGCCR 4 cut(s) 185, 191, 1347, 3016
AcsI RAATTY 8 cut(s) 502, 534, 1376, 1658, 2656, 2876, 3264, 3420
AcuI CTGAAG 2 cut(s) 1728, 1869
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 8 cut(s) 333, 354, 898, 923, 1345, 2353, 3258, 3294
AhdI GACNNNNNGTC 2 cut(s) 16, 316
AjnI CCWGG 4 cut(s) 1145, 1191, 1778, 3257
AjuI GAANNNNNNNTTGG 8 cut(s) 77, 109, 857, 889, 1314, 1346, 1467, 1499
AloI GAACNNNNNNTCC 2 cut(s) 2799, 2831
Alw21I GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
Alw26I GTCTC 8 cut(s) 64, 243, 303, 368, 446, 666, 1032, 2905
AlwI GGATC 4 cut(s) 222, 235, 1253, 2632
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 2 cut(s) 766, 822
AoxI GGCC 7 cut(s) 14, 185, 191, 363, 1347, 1965, 3016
ApeKI GCWGC 8 cut(s) 479, 749, 913, 1205, 2279, 2750, 2768, 2905
ApoI RAATTY 8 cut(s) 502, 534, 1376, 1658, 2656, 2876, 3264, 3420
ArsI GACNNNNNNTTYG 2 cut(s) 3344, 3376
AseI ATTAAT 1 cut(s) 1055
Asp700I GAANNNNTTC 1 cut(s) 2487
AspLEI GCGC 4 cut(s) 302, 304, 651, 1481
AspS9I GGNCC 5 cut(s) 363, 1491, 1966, 2175, 2505
AsuC2I CCSGG 2 cut(s) 499, 592
AsuHPI GGTGA 4 cut(s) 88, 707, 749, 1769
AsuNHI GCTAGC 1 cut(s) 2548
AvaI CYCGRG 2 cut(s) 766, 822
AvaII GGWCC 3 cut(s) 1491, 2175, 2505
BalI TGGCCA 2 cut(s) 1349, 3018
BamHI GGATCC 1 cut(s) 227
BanII GRGCYC 2 cut(s) 1958, 3134
BbsI GAAGAC 2 cut(s) 1007, 2018
Bbv12I GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
BbvCI CCTCAGC 1 cut(s) 2538
BbvI GCAGC 8 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755, 2892
BceAI ACGGC 6 cut(s) 7, 110, 184, 200, 328, 2331
BcgI CGANNNNNNTGC 4 cut(s) 110, 144, 3128, 3162
BciT130I CCWGG 4 cut(s) 1147, 1193, 1780, 3259
BciVI GTATCC 1 cut(s) 676
BclI TGATCA 3 cut(s) 1819, 2440, 2740
BcnI CCSGG 2 cut(s) 499, 592
BcoDI GTCTC 8 cut(s) 64, 243, 303, 368, 446, 666, 1032, 2905
BfmI CTRYAG 1 cut(s) 445
BfoI RGCGCY 2 cut(s) 652, 1482
BfuAI ACCTGC 2 cut(s) 1219, 1318
BfuI GTATCC 1 cut(s) 676
BglII AGATCT 1 cut(s) 3364
BmcAI AGTACT 2 cut(s) 2457, 3444
Bme1390I CCNGG 6 cut(s) 499, 592, 1147, 1193, 1780, 3259
Bme18I GGWCC 3 cut(s) 1491, 2175, 2505
BmeRI GACNNNNNGTC 2 cut(s) 16, 316
BmeT110I CYCGRG 2 cut(s) 766, 822
BmgT120I GGNCC 5 cut(s) 363, 1491, 1966, 2175, 2505
BmiI GGNNCC 2 cut(s) 229, 2176
BmrFI CCNGG 6 cut(s) 499, 592, 1147, 1193, 1780, 3259
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 8 cut(s) 544, 556, 859, 1713, 1818, 2371, 2892, 3200
BmtI GCTAGC 1 cut(s) 2552
BmuI ACTGGG 1 cut(s) 29
BoxI GACNNNNGTC 1 cut(s) 3066
BpiI GAAGAC 2 cut(s) 1007, 2018
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 3 cut(s) 759, 1129, 1239
Bpu10I CCTNAGC 3 cut(s) 147, 2063, 2538
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 2 cut(s) 499, 592
BsaAI YACGTR 2 cut(s) 2258, 2726
BsaBI GATNNNNATC 1 cut(s) 1818
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 5 cut(s) 188, 591, 1237, 2680, 3293
Bsc4I CCNNNNNNNGG 8 cut(s) 333, 354, 898, 923, 1345, 2353, 3258, 3294
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 5 cut(s) 35, 348, 408, 776, 1759
Bse3DI GCAATG 5 cut(s) 456, 2075, 2598, 2841, 3216
Bse8I GATNNNNATC 1 cut(s) 1818
BseBI CCWGG 4 cut(s) 1147, 1193, 1780, 3259
BseDI CCNNGG 5 cut(s) 188, 591, 1237, 2680, 3293
BseGI GGATG 8 cut(s) 443, 456, 580, 725, 850, 1833, 2801, 3322
BseJI GATNNNNATC 1 cut(s) 1818
BseLI CCNNNNNNNGG 8 cut(s) 333, 354, 898, 923, 1345, 2353, 3258, 3294
BseMI GCAATG 5 cut(s) 456, 2075, 2598, 2841, 3216
BseNI ACTGG 5 cut(s) 35, 348, 408, 776, 1759
BsePI GCGCGC 1 cut(s) 300
BseRI GAGGAG 5 cut(s) 182, 295, 1394, 1556, 2505
BseX3I CGGCCG 2 cut(s) 185, 191
BseXI GCAGC 8 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755, 2892
BseYI CCCAGC 1 cut(s) 2943
Bsh1236I CGCG 2 cut(s) 190, 302
Bsh1285I CGRYCG 2 cut(s) 188, 194
BshFI GGCC 7 cut(s) 16, 187, 193, 365, 1349, 1967, 3018
BsiEI CGRYCG 2 cut(s) 188, 194
BsiHKAI GWGCWC 5 cut(s) 931, 1440, 1958, 2353, 2677
BsiHKCI CYCGRG 2 cut(s) 766, 822
BsiSI CCGG 4 cut(s) 164, 327, 498, 591
BsiWI CGTACG 2 cut(s) 181, 201
BslFI GGGAC 3 cut(s) 53, 1888, 2188
BslI CCNNNNNNNGG 8 cut(s) 333, 354, 898, 923, 1345, 2353, 3258, 3294
BsmAI GTCTC 8 cut(s) 64, 243, 303, 368, 446, 666, 1032, 2905
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 53, 1888, 2188
BsmI GAATGC 1 cut(s) 1221
BsnI GGCC 7 cut(s) 16, 187, 193, 365, 1349, 1967, 3018
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 2 cut(s) 766, 822
Bsp1286I GDGCHC 6 cut(s) 931, 1440, 1958, 2353, 2677, 3134
Bsp19I CCATGG 1 cut(s) 3293
BspANI GGCC 7 cut(s) 16, 187, 193, 365, 1349, 1967, 3018
BspFNI CGCG 2 cut(s) 190, 302
BspHI TCATGA 2 cut(s) 856, 2113
BspLI GGNNCC 2 cut(s) 229, 2176
BspMI ACCTGC 2 cut(s) 1219, 1318
BspOI GCTAGC 1 cut(s) 2552
BspPI GGATC 4 cut(s) 222, 235, 1253, 2632
BspQI GCTCTTC 1 cut(s) 2289
BspTNI GGTCTC 1 cut(s) 666
BsrBI CCGCTC 1 cut(s) 1355
BsrDI GCAATG 5 cut(s) 456, 2075, 2598, 2841, 3216
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 5 cut(s) 35, 348, 408, 776, 1759
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 5 cut(s) 188, 591, 1237, 2680, 3293
BssHII GCGCGC 1 cut(s) 300
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 3 cut(s) 1237, 2680, 3293
Bst2UI CCWGG 4 cut(s) 1147, 1193, 1780, 3259
Bst6I CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
BstACI GRCGYC 1 cut(s) 123
BstBAI YACGTR 2 cut(s) 2258, 2726
BstC8I GCNNGC 9 cut(s) 238, 302, 931, 935, 1210, 2550, 2677, 3241, 3289
BstDSI CCRYGG 2 cut(s) 188, 3293
BstF5I GGATG 8 cut(s) 443, 456, 580, 725, 850, 1833, 2801, 3322
BstFNI CGCG 2 cut(s) 190, 302
BstH2I RGCGCY 2 cut(s) 652, 1482
BstHHI GCGC 4 cut(s) 302, 304, 651, 1481
BstMAI GTCTC 8 cut(s) 64, 243, 303, 368, 446, 666, 1032, 2905
BstMCI CGRYCG 2 cut(s) 188, 194
BstNI CCWGG 4 cut(s) 1147, 1193, 1780, 3259
BstNSI RCATGY 4 cut(s) 1127, 1187, 1212, 3243
BstPAI GACNNNNGTC 1 cut(s) 3066
BstSCI CCNGG 6 cut(s) 497, 590, 1145, 1191, 1778, 3257
BstSFI CTRYAG 1 cut(s) 445
BstUI CGCG 2 cut(s) 190, 302
BstV1I GCAGC 8 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755, 2892
BstV2I GAAGAC 2 cut(s) 1007, 2018
BstX2I RGATCY 3 cut(s) 227, 1258, 3364
BstXI CCANNNNNNTGG 1 cut(s) 1501
BstYI RGATCY 3 cut(s) 227, 1258, 3364
BstZI CGGCCG 2 cut(s) 185, 191
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 7 cut(s) 16, 187, 193, 365, 1349, 1967, 3018
BtgI CCRYGG 2 cut(s) 188, 3293
BtgZI GCGATG 2 cut(s) 255, 3223
BtsCI GGATG 8 cut(s) 443, 456, 580, 725, 850, 1833, 2801, 3322
BtsIMutI CAGTG 6 cut(s) 355, 415, 1407, 1456, 2027, 2712
BveI ACCTGC 2 cut(s) 1219, 1318
Cac8I GCNNGC 9 cut(s) 238, 302, 931, 935, 1210, 2550, 2677, 3241, 3289
CaiI CAGNNNCTG 1 cut(s) 943
CciI TCATGA 2 cut(s) 856, 2113
CfoI GCGC 4 cut(s) 302, 304, 651, 1481
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 5 cut(s) 363, 1491, 1966, 2175, 2505
Cfr42I CCGCGG 1 cut(s) 191
CseI GACGC 2 cut(s) 131, 1778
DraI TTTAAA 3 cut(s) 508, 2370, 2641
DrdI GACNNNNNNGTC 3 cut(s) 382, 1579, 2919
DriI GACNNNNNGTC 2 cut(s) 16, 316
DseDI GACNNNNNNGTC 3 cut(s) 382, 1579, 2919
EaeI YGGCCR 4 cut(s) 185, 191, 1347, 3016
EagI CGGCCG 2 cut(s) 185, 191
Eam1104I CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
Eam1105I GACNNNNNGTC 2 cut(s) 16, 316
EarI CTCTTC 7 cut(s) 272, 284, 2289, 2410, 2446, 2523, 2775
EciI GGCGGA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 1956
EclXI CGGCCG 2 cut(s) 185, 191
Eco130I CCWWGG 3 cut(s) 1237, 2680, 3293
Eco24I GRGCYC 2 cut(s) 1958, 3134
Eco31I GGTCTC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 2028
Eco47I GGWCC 3 cut(s) 1491, 2175, 2505
Eco52I CGGCCG 2 cut(s) 185, 191
Eco53kI GAGCTC 1 cut(s) 1956
Eco57I CTGAAG 2 cut(s) 1728, 1869
Eco88I CYCGRG 2 cut(s) 766, 822
EcoICRI GAGCTC 1 cut(s) 1956
EcoRI GAATTC 1 cut(s) 1376
EcoRII CCWGG 4 cut(s) 1145, 1191, 1778, 3257
EcoRV GATATC 1 cut(s) 2028
EcoT14I CCWWGG 3 cut(s) 1237, 2680, 3293
EcoT22I ATGCAT 2 cut(s) 571, 2598
EcoT38I GRGCYC 2 cut(s) 1958, 3134
ErhI CCWWGG 3 cut(s) 1237, 2680, 3293
Esp3I CGTCTC 1 cut(s) 303
FalI AAGNNNNNCTT 6 cut(s) 1467, 1499, 1887, 1919, 2407, 2439
FaqI GGGAC 3 cut(s) 53, 1888, 2188
FauI CCCGC 5 cut(s) 308, 1270, 1865, 2333, 3375
FauNDI CATATG 1 cut(s) 3223
FbaI TGATCA 3 cut(s) 1819, 2440, 2740
FokI GGATG 8 cut(s) 450, 463, 587, 712, 837, 1840, 2808, 3329
FriOI GRGCYC 2 cut(s) 1958, 3134
GlaI GCGC 4 cut(s) 301, 303, 650, 1480
GsaI CCCAGC 1 cut(s) 2947
GsuI CTGGAG 3 cut(s) 759, 1129, 1239
HaeII RGCGCY 2 cut(s) 652, 1482
HaeIII GGCC 7 cut(s) 16, 187, 193, 365, 1349, 1967, 3018
HapII CCGG 4 cut(s) 164, 327, 498, 591
HgaI GACGC 2 cut(s) 131, 1778
HhaI GCGC 4 cut(s) 302, 304, 651, 1481
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 4 cut(s) 300, 302, 649, 1479
HinP1I GCGC 4 cut(s) 300, 302, 649, 1479
HincII GTYRAC 1 cut(s) 2787
HindII GTYRAC 1 cut(s) 2787
HindIII AAGCTT 2 cut(s) 510, 1710
HpaI GTTAAC 1 cut(s) 2787
HpaII CCGG 4 cut(s) 164, 327, 498, 591
HphI GGTGA 4 cut(s) 88, 707, 749, 1769
Hpy166II GTNNAC 4 cut(s) 581, 659, 1494, 2787
Hpy8I GTNNAC 4 cut(s) 581, 659, 1494, 2787
Hpy99I CGWCG 6 cut(s) 11, 22, 28, 125, 182, 388
HpyCH4IV ACGT 5 cut(s) 66, 180, 2257, 2725, 3032
HpySE526I ACGT 5 cut(s) 66, 180, 2257, 2725, 3032
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 4 cut(s) 300, 302, 649, 1479
Ksp22I TGATCA 3 cut(s) 1819, 2440, 2740
KspAI GTTAAC 1 cut(s) 2787
KspI CCGCGG 1 cut(s) 191
LguI GCTCTTC 1 cut(s) 2289
LmnI GCTCC 7 cut(s) 169, 297, 500, 847, 1407, 2518, 2545
Lsp1109I GCAGC 8 cut(s) 466, 736, 925, 1192, 2266, 2737, 2755, 2892
LweI GCATC 8 cut(s) 544, 556, 859, 1713, 1818, 2371, 2892, 3200
MaeII ACGT 5 cut(s) 66, 180, 2257, 2725, 3032
MaeIII GTNAC 3 cut(s) 759, 1983, 2335
MbiI CCGCTC 1 cut(s) 1355
MfeI CAATTG 1 cut(s) 2663
MflI RGATCY 3 cut(s) 227, 1258, 3364
MhlI GDGCHC 6 cut(s) 931, 1440, 1958, 2353, 2677, 3134
MlsI TGGCCA 2 cut(s) 1349, 3018
MluNI TGGCCA 2 cut(s) 1349, 3018
MlyI GAGTC 5 cut(s) 767, 814, 934, 1920, 2957
MmeI TCCRAC 5 cut(s) 108, 366, 1447, 1482, 1550
Mox20I TGGCCA 2 cut(s) 1349, 3018
Mph1103I ATGCAT 2 cut(s) 571, 2598
MroXI GAANNNNTTC 1 cut(s) 2487
MscI TGGCCA 2 cut(s) 1349, 3018
MslI CAYNNNNRTG 4 cut(s) 855, 1557, 2020, 2900
Msp20I TGGCCA 2 cut(s) 1349, 3018
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 4 cut(s) 164, 327, 498, 591
MspR9I CCNGG 6 cut(s) 499, 592, 1147, 1193, 1780, 3259
MunI CAATTG 1 cut(s) 2663
Mva1269I GAATGC 1 cut(s) 1221
MvaI CCWGG 4 cut(s) 1147, 1193, 1780, 3259
MvnI CGCG 2 cut(s) 190, 302
NciI CCSGG 2 cut(s) 499, 592
NcoI CCATGG 1 cut(s) 3293
NdeI CATATG 1 cut(s) 3223
NheI GCTAGC 1 cut(s) 2548
NlaIV GGNNCC 2 cut(s) 229, 2176
NmuCI GTSAC 1 cut(s) 759
NsiI ATGCAT 2 cut(s) 571, 2598
NspI RCATGY 4 cut(s) 1127, 1187, 1212, 3243
PaeI GCATGC 2 cut(s) 1212, 3243
PagI TCATGA 2 cut(s) 856, 2113
PauI GCGCGC 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 2289
PctI GAATGC 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 2487
Pfl23II CGTACG 2 cut(s) 181, 201
PflMI CCANNNNNTGG 1 cut(s) 1345
PfoI TCCNGGA 1 cut(s) 497
PleI GAGTC 5 cut(s) 766, 814, 934, 1919, 2956
PpsI GAGTC 5 cut(s) 766, 814, 934, 1919, 2956
Ppu21I YACGTR 2 cut(s) 2258, 2726
PshAI GACNNNNGTC 1 cut(s) 3066
PshBI ATTAAT 1 cut(s) 1055
PsiI TTATAA 1 cut(s) 1029
Psp124BI GAGCTC 1 cut(s) 1958
Psp6I CCWGG 4 cut(s) 1145, 1191, 1778, 3257
PspFI CCCAGC 1 cut(s) 2943
PspGI CCWGG 4 cut(s) 1145, 1191, 1778, 3257
PspLI CGTACG 2 cut(s) 181, 201
PspN4I GGNNCC 2 cut(s) 229, 2176
PspPI GGNCC 5 cut(s) 363, 1491, 1966, 2175, 2505
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 3 cut(s) 227, 1258, 3364
PteI GCGCGC 1 cut(s) 300
RseI CAYNNNNRTG 4 cut(s) 855, 1557, 2020, 2900
SacI GAGCTC 1 cut(s) 1958
SacII CCGCGG 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 2289
Sau96I GGNCC 5 cut(s) 363, 1491, 1966, 2175, 2505
ScaI AGTACT 2 cut(s) 2457, 3444
SchI GAGTC 5 cut(s) 767, 814, 934, 1920, 2957
ScrFI CCNGG 6 cut(s) 499, 592, 1147, 1193, 1780, 3259
SduI GDGCHC 6 cut(s) 931, 1440, 1958, 2353, 2677, 3134
SfaNI GCATC 8 cut(s) 544, 556, 859, 1713, 1818, 2371, 2892, 3200
SfcI CTRYAG 1 cut(s) 445
Sfr303I CCGCGG 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 191
SinI GGWCC 3 cut(s) 1491, 2175, 2505
SmiMI CAYNNNNRTG 4 cut(s) 855, 1557, 2020, 2900
SmlI CTYRAG 1 cut(s) 1652
SmoI CTYRAG 1 cut(s) 1652
SphI GCATGC 2 cut(s) 1212, 3243
SspI AATATT 1 cut(s) 2437
SstI GAGCTC 1 cut(s) 1958
StyD4I CCNGG 6 cut(s) 497, 590, 1145, 1191, 1778, 3257
StyI CCWWGG 3 cut(s) 1237, 2680, 3293
TaiI ACGT 5 cut(s) 69, 183, 2260, 2728, 3035
TaqI TCGA 4 cut(s) 338, 463, 786, 1907
TaqII GACCGA 1 cut(s) 2559
TatI WGTACW 4 cut(s) 615, 1648, 2455, 3442
TauI GCSGC 3 cut(s) 47, 190, 193
TscAI CASTG 6 cut(s) 355, 415, 1414, 1456, 2027, 2712
TseFI GTSAC 1 cut(s) 759
TseI GCWGC 8 cut(s) 479, 749, 913, 1205, 2279, 2750, 2768, 2905
Tsp45I GTSAC 1 cut(s) 759
TspGWI ACGGA 3 cut(s) 100, 728, 2625
TspRI CASTG 6 cut(s) 355, 415, 1414, 1456, 2027, 2712
Van91I CCANNNNNTGG 1 cut(s) 1345
VpaK11BI GGWCC 3 cut(s) 1491, 2175, 2505
VspI ATTAAT 1 cut(s) 1055
XapI RAATTY 8 cut(s) 502, 534, 1376, 1658, 2656, 2876, 3264, 3420
XbaI TCTAGA 1 cut(s) 2818
XceI RCATGY 4 cut(s) 1127, 1187, 1212, 3243
XmnI GAANNNNTTC 1 cut(s) 2487
ZrmI AGTACT 2 cut(s) 2457, 3444
Zsp2I ATGCAT 2 cut(s) 571, 2598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.