Rh7CG008200

Protein NRDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0010115
Physical Location & Seq
Reverse (-)
611009 .. 617824
6816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG008200.1

Sequence Viewer

Length: 3492 bp
ATGGACGACGACAGGCCGTCGGAAGAAGAAGCGAAGCCGTCGCCGTCGCCGTCGCCGTCGCTGTCGCTGTTCCCAGTCATAGCCGCCTCTCAGCAAGTCTCAAACGTCCCTCAATGGCTTTCCAACACCAGCTTCACCACCAACCTCTCTGTCATAAACGACGCCGTTGCCTCGCACTTCAAACCTGAGCCACCGCCGGAGGAGCAAGACGACGTACGGCCCCAGCCGAAGCCGTACGAGCTTCTGGAATCTTCTAGCGGATCCGAAGCGAGCGATGAGAGAGACAGGACGAGCAAGAAGAGGAGGAAGAAGGAGAAGGGGAAGAGGAGGCGGAGCGCGCAGAGAGACGGCGGGTCGTTCGCCGGTTTCGGGTCGAGAAAGTCCAGTGTTCGGGCTTGGGCCGAATCCGAGACTAGACCGTCGGAAAATTATTACTTGGATTCCAGTGGCGACCTTGATAATTTGGCCTTTGGATGTCTCTATAGGATGGACATTGCTCGATACAAACCTTATGCTGCTCTTTCGGATAGCTCCGGGAATTTTAAAGCTTTGTATCAGGGGAATCAGACAATTTCAACATTGGATAGAGATGCTGATGCTGATGCATTGGATGGTAAACTAAAATCTGGGGGTCGCTATTGGTCTGCAAAGTACATGGCTTTGGAACGCCATAAGAACTTAAAGCGCCTTCGTTTACTTGCCCCCAGAGACCTTGCGGATACAGTTTCAGGTGATTTTATTCCTTTGACAGATATTCAAACATCCGATGAAGATGAAGGTGTTGCTGCTGATGAGTCACTCTCGGGAACTCCAGTAGTGGTCGAAGAATCATGGGAAGATGAATTGCTACGCAAGACTCGGGAGTTTAACAAATTGACAAGGGAGCATCCTCATGATGAGAAGGTTTGGTTGGCTTTTGCTGAGTTCCAAGACAAGGTTGCTGATATGCAGCCCCAGAAAGGTGCTCGCTTGCAGACTCTGGAAAAAAAGATTAGCATATTGGAGAAAGCCACAGAGCTTAACCCAGATAATGAAGAACTGTTGCTTTGTCTTCTAAAGACTTATAAAAGCAGAGACAGTTCCGATGTATTAATTAGTAGATGGGAAAAGATACTCATACAGCATTCAGGAAATTACCACTTGTGGAGAGAGTTTCTGCATGTTATTCAAGGGGAGTTCTCCAGGTTCAAGGTTTCAGACATGAGAAAAATGTACGCACATGCAATCCAGGCTATATCTGCTGCATGCAGAATGCACTCTAGGCAGGTTTGCCAAGGAGAAAAATCTTCTCCAGATCCCGCTATTGTTCAACTAGAACTTGGTCTGGTGGATATTTTTCTCAGTTATTGTAGGTTTGAATGGCAGGTTGGCTACCAAGAGTTGGCCACCGCTCTATTTCAGGCTGAAATTGAATTCAGTTTGTTTTGTCCTTCTTTGCTCCTCACTGAGCAGAGTAAGCAGATATTATTTGAGCACTTCTGGAACAGTGATGGTGCTAGAGTTGGAGAAGAAGGCGCTCTTGGTTGGTCCACATGGTTGGAGAAAGAGGAAGAAAATAGGCAAAGGGTCATCAGAGAGGAGATGGCACATGATAATGAAGGTGGTTGGACAGGTTGGTCTGAACCACTATCAAAAAATAAGGAAGGTAGTACCAATCCAGAAAAGGAGGCTGAAAGTAATGCTGTACTTGAGGAATTTCAAGAAGAAACTGAGAACGAAGATGTCAAGCAAGAAGATGATACTGAAGCTTTGCTAAAGATGCTCGGTATTGATGTTGATGTTGATGTTGGGGCTACTGGTGAGGGTAAAGACGCTTCAACCTGGATTAGATGGTCAGAAGAAGAAAAATCAAGAGATTGTGATCAATGGATGCCTGTTCGTGCAAAATCTGAAGCTGTTGCCTCTAATAATGGTGGGACACCTGATAGAGAAGCAGAAGAACACCTTTCGAGAGTCATAATGTATGAAGATGTTGCTGAGTACCTCTTTTCTTTGAGCTCATTTGAGGCCCGTTTATCTCTAGTGTTACAGTTCGTTGATTTCTTTGGTGGGAAGTCATCTCAGTGGATATCTACAAACAGTTCAGCTTGGTCTGAGAAACTCCTTAGCCTGGAGGCATTGCCACATTCAGTATTACACACTCTGAAAAGGGTTCATGATGTCTTAAGCAAAACACAAGGTAGTTCAAATAGTTTCAGCTTGGAATCTCTGTTGGGGACCACCAATGACATCCATGGAAAGGCAGACCTGATGAAGTTTCTTTGTAATGCTACCTTGCTCTGTTTATCTGCTTTTCCACGTAATTATCTCCTAGAGGAAGCTGCTTTAGTTGCAGAAGAGCTATCTGTTGTGAATCTGAATCCATCCTGCAGTTCAGTTACCCCCTGCCGTGCTCTGGCAAAGTATCTTTTAAAGAGTGATCGTCAGGATATATTGCTATGTGGTGTTTATGCAAGAAGAGAGGCTTTTTATGGGAATATTGATCATGCAAGAAGAGTATTTGACATGGCGTTGTCATCTATTGAAGGGCTTCCTTTGGAATTGAGGTCCAATGCTCCTCTTCTGTATTTCTGGTATGCTGAGGTGGAGCTAGCCAAAGATCAGGGAAATCAGTCTGAATCATCATTTCGTGCAATGCATATTTTATCTTGCTTAGGAAGTGGTGGATCATATAGTCCGTTTAAATGTCAGCCATCAAATTTGCAATTGCTGAGAGCACGCCAAGGCTTCAAAGAAAGAATAAGAACAGTGCAAATGTCATGGGTACGTGGTGTTATAGATGATCAATCCGCTGCTCTCGTATGTTGTGCTGCTTTATTTGAAGAGTTAACTTCTGGATGGGCTTTGGGAATTGAAGTTCTAGATCATGCTTTTGCAATGGTGCTTCCAGAGAGAAAAAGCCATAGCCATCAACTTGAATTTGTGTTCAACTTTTACATGAAGATGCTGTGGAGACATCATGGTCAATCAAGTCTATCAAAATGCTGGGAGTCCATTTTGCAGGGGCTTCAAATATATTCATTCAGCCCTGAACTTTATAGTGATCTAATTGAGGTTGGCCATTTTTATACAACGTCCAATAAACTGCGGTGGGTGTTTGATGACTACTGTCAGAAGAAACCATCTGTTGTCGTCTGGCTTTTTGCCTTGTCATTTGAGATAAGCAAAGGGGGCTCACAGCATAGGATTCGTGGATTGTTTGAAAGGGCATTGGCAAATGATAGGTTTCATAACTCGGTTGTGCTGTGGCGATGCTACATTGCATATGAAATGAACATAGCATGCAATCCGTCCATATCCAGGCGAATTTTCTTCCGAGCTATCCATGCCTGCCCATGGTCAAAGAAGCTATGGCTGGATGGTTTTCTCAAATTGAACTCCACCCTATCTGCAAAAGAGCTGTCAGATCTCCAAGAAGTTATGCGGGATAAGGAACTGAATCTGAGAACAGACATTTATGAAATTCTTTTGCAAGATGAGCTAGTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NRDE-2

Protein Analysis

1163

Amino Acids

132.14

Weight (kDa)

5.28

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NRDE-2 PF08424 285 - 684 6.9e-76 NRDE-2, necessary for RNA interference
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010115)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17712 AT3G17712 AT3G17712 AT3G17740
fragaria_vesca FvH4_5g17940 FvH4_5g17940 FvH4_5g17940
malus_domestica MD14G1242200.v1.1
prunus_persica Prupe.5G239800_v2.0.a1
pyrus_communis pycom14g20360
rosa_chinensis RchiOBHm_Chr7g0177661
rosa_laevigata RLG00000005516
rosa_multiflora Rmu_sc0004449.1_g000002 Rmu_ssc0000236.1_g000002
rosa_roxburghii Rroxscaffold_3G00275680
rosa_rugosa Rorug06G0408300
rosa_samantha Rh7AG007800 Rh7BG007700 Rh7CG008200 Rh7DG007900
rosa_wichuraiana Rw7G000650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AasI GACNNNNNNGTC 3 cut(s) 418, 1615, 2961
Acc36I ACCTGC 2 cut(s) 1255, 1354
AccB7I CCANNNNNTGG 1 cut(s) 1381
AccBSI CCGCTC 1 cut(s) 1391
AccII CGCG 1 cut(s) 338
AclWI GGATC 4 cut(s) 255, 268, 1289, 2674
AcoI YGGCCR 2 cut(s) 1383, 3058
AcsI RAATTY 7 cut(s) 538, 1412, 1694, 2698, 2918, 3306, 3462
AcuI CTGAAG 2 cut(s) 1764, 1911
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 9 cut(s) 216, 236, 653, 1214, 1651, 1686, 1982, 2766, 3486
AflII CTTAAG 1 cut(s) 2164
AhdI GACNNNNNGTC 2 cut(s) 16, 352
AjnI CCWGG 5 cut(s) 1181, 1227, 1820, 2109, 3299
AjuI GAANNNNNNNTTGG 8 cut(s) 116, 148, 893, 925, 1350, 1382, 1503, 1535
AloI GAACNNNNNNTCC 2 cut(s) 2841, 2873
Alw21I GWGCWC 5 cut(s) 967, 1476, 2000, 2395, 2719
Alw26I GTCTC 8 cut(s) 103, 276, 339, 404, 482, 702, 1068, 2947
AlwI GGATC 4 cut(s) 255, 268, 1289, 2674
AlwNI CAGNNNCTG 1 cut(s) 979
Ama87I CYCGRG 2 cut(s) 802, 858
AoxI GGCC 7 cut(s) 14, 218, 399, 465, 1383, 2007, 3058
ApeKI GCWGC 7 cut(s) 515, 785, 949, 1241, 2321, 2792, 2810
ApoI RAATTY 7 cut(s) 538, 1412, 1694, 2698, 2918, 3306, 3462
ArsI GACNNNNNNTTYG 2 cut(s) 3386, 3418
AseI ATTAAT 1 cut(s) 1091
Asp700I GAANNNNTTC 1 cut(s) 2529
AspLEI GCGC 4 cut(s) 338, 340, 687, 1517
AspS9I GGNCC 6 cut(s) 219, 399, 1527, 2008, 2217, 2547
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 3 cut(s) 127, 743, 1811
AsuNHI GCTAGC 1 cut(s) 2590
AvaI CYCGRG 2 cut(s) 802, 858
AvaII GGWCC 3 cut(s) 1527, 2217, 2547
BalI TGGCCA 2 cut(s) 1385, 3060
BamHI GGATCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 2000, 3176
BarI GAAGNNNNNNTAC 2 cut(s) 1798, 1830
BbsI GAAGAC 1 cut(s) 1043
Bbv12I GWGCWC 5 cut(s) 967, 1476, 2000, 2395, 2719
BbvCI CCTCAGC 1 cut(s) 2580
BbvI GCAGC 7 cut(s) 502, 772, 961, 1228, 2308, 2779, 2797
BceAI ACGGC 9 cut(s) 22, 28, 34, 40, 149, 217, 233, 364, 2373
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 3170, 3204
BciT130I CCWGG 5 cut(s) 1183, 1229, 1822, 2111, 3301
BciVI GTATCC 1 cut(s) 712
BclI TGATCA 3 cut(s) 1861, 2482, 2782
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 8 cut(s) 103, 276, 339, 404, 482, 702, 1068, 2947
BfmI CTRYAG 2 cut(s) 481, 2368
BfoI RGCGCY 2 cut(s) 688, 1518
BfrI CTTAAG 1 cut(s) 2164
BfuAI ACCTGC 2 cut(s) 1255, 1354
BfuI GTATCC 1 cut(s) 712
BglII AGATCT 1 cut(s) 3406
BisI GCNGC 8 cut(s) 84, 516, 786, 950, 1242, 2322, 2793, 2811
BlsI GCNGC 8 cut(s) 85, 517, 787, 951, 1243, 2323, 2794, 2812
BmcAI AGTACT 1 cut(s) 3486
Bme1390I CCNGG 6 cut(s) 535, 1183, 1229, 1822, 2111, 3301
Bme18I GGWCC 3 cut(s) 1527, 2217, 2547
BmeRI GACNNNNNGTC 2 cut(s) 16, 352
BmeT110I CYCGRG 2 cut(s) 802, 858
BmgT120I GGNCC 6 cut(s) 219, 399, 1527, 2008, 2217, 2547
BmiI GGNNCC 3 cut(s) 221, 262, 2218
BmrFI CCNGG 6 cut(s) 535, 1183, 1229, 1822, 2111, 3301
BmrI ACTGGG 1 cut(s) 68
BmsI GCATC 8 cut(s) 580, 586, 592, 895, 1749, 1860, 2934, 3242
BmtI GCTAGC 1 cut(s) 2594
BmuI ACTGGG 1 cut(s) 68
BoxI GACNNNNGTC 1 cut(s) 3108
BpiI GAAGAC 1 cut(s) 1043
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 4 cut(s) 795, 1165, 1275, 2132
Bpu10I CCTNAGC 4 cut(s) 186, 2105, 2580, 2653
BpuEI CTTGAG 1 cut(s) 1709
BpuMI CCSGG 1 cut(s) 535
BsaAI YACGTR 2 cut(s) 2300, 2768
BsaBI GATNNNNATC 1 cut(s) 1860
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 1273, 2233, 2722, 3335
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 5 cut(s) 74, 384, 444, 812, 1801
Bse3DI GCAATG 5 cut(s) 492, 2117, 2640, 2883, 3258
Bse8I GATNNNNATC 1 cut(s) 1860
BseBI CCWGG 5 cut(s) 1183, 1229, 1822, 2111, 3301
BseDI CCNNGG 4 cut(s) 1273, 2233, 2722, 3335
BseJI GATNNNNATC 1 cut(s) 1860
BseMI GCAATG 5 cut(s) 492, 2117, 2640, 2883, 3258
BseNI ACTGG 5 cut(s) 74, 384, 444, 812, 1801
BsePI GCGCGC 1 cut(s) 336
BseRI GAGGAG 6 cut(s) 215, 316, 340, 1430, 1592, 2547
BseXI GCAGC 7 cut(s) 502, 772, 961, 1228, 2308, 2779, 2797
BseYI CCCAGC 2 cut(s) 222, 2985
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 7 cut(s) 16, 220, 401, 467, 1385, 2009, 3060
BsiHKAI GWGCWC 5 cut(s) 967, 1476, 2000, 2395, 2719
BsiHKCI CYCGRG 2 cut(s) 802, 858
BsiSI CCGG 3 cut(s) 197, 363, 534
BsiWI CGTACG 2 cut(s) 214, 234
BslFI GGGAC 3 cut(s) 92, 1930, 2230
BsmAI GTCTC 8 cut(s) 103, 276, 339, 404, 482, 702, 1068, 2947
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 3 cut(s) 92, 1930, 2230
BsmI GAATGC 2 cut(s) 1123, 1257
BsnI GGCC 7 cut(s) 16, 220, 401, 467, 1385, 2009, 3060
Bso31I GGTCTC 1 cut(s) 702
BsoBI CYCGRG 2 cut(s) 802, 858
Bsp1286I GDGCHC 6 cut(s) 967, 1476, 2000, 2395, 2719, 3176
Bsp19I CCATGG 2 cut(s) 2233, 3335
BspANI GGCC 7 cut(s) 16, 220, 401, 467, 1385, 2009, 3060
BspFNI CGCG 1 cut(s) 338
BspHI TCATGA 2 cut(s) 892, 2155
BspLI GGNNCC 3 cut(s) 221, 262, 2218
BspMAI CTGCAG 1 cut(s) 2372
BspMI ACCTGC 2 cut(s) 1255, 1354
BspOI GCTAGC 1 cut(s) 2594
BspPI GGATC 4 cut(s) 255, 268, 1289, 2674
BspQI GCTCTTC 1 cut(s) 2331
BspTI CTTAAG 1 cut(s) 2164
BspTNI GGTCTC 1 cut(s) 702
BsrBI CCGCTC 1 cut(s) 1391
BsrDI GCAATG 5 cut(s) 492, 2117, 2640, 2883, 3258
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 5 cut(s) 74, 384, 444, 812, 1801
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 4 cut(s) 1273, 2233, 2722, 3335
BssHII GCGCGC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 4 cut(s) 1273, 2233, 2722, 3335
Bst2UI CCWGG 5 cut(s) 1183, 1229, 1822, 2111, 3301
Bst6I CTCTTC 7 cut(s) 293, 317, 2331, 2452, 2488, 2565, 2817
BstACI GRCGYC 1 cut(s) 162
BstAFI CTTAAG 1 cut(s) 2164
BstBAI YACGTR 2 cut(s) 2300, 2768
BstC8I GCNNGC 9 cut(s) 271, 338, 967, 971, 1246, 2592, 2719, 3283, 3331
BstDSI CCRYGG 2 cut(s) 2233, 3335
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 2 cut(s) 688, 1518
BstHHI GCGC 4 cut(s) 338, 340, 687, 1517
BstMAI GTCTC 8 cut(s) 103, 276, 339, 404, 482, 702, 1068, 2947
BstNI CCWGG 5 cut(s) 1183, 1229, 1822, 2111, 3301
BstNSI RCATGY 4 cut(s) 1163, 1223, 1248, 3285
BstPAI GACNNNNGTC 1 cut(s) 3108
BstSCI CCNGG 6 cut(s) 533, 1181, 1227, 1820, 2109, 3299
BstSFI CTRYAG 2 cut(s) 481, 2368
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 7 cut(s) 502, 772, 961, 1228, 2308, 2779, 2797
BstV2I GAAGAC 1 cut(s) 1043
BstX2I RGATCY 3 cut(s) 260, 1294, 3406
BstXI CCANNNNNNTGG 1 cut(s) 1537
BstYI RGATCY 3 cut(s) 260, 1294, 3406
BsuI GTATCC 1 cut(s) 712
BsuRI GGCC 7 cut(s) 16, 220, 401, 467, 1385, 2009, 3060
BtgI CCRYGG 2 cut(s) 2233, 3335
BtgZI GCGATG 2 cut(s) 288, 3265
BtsIMutI CAGTG 6 cut(s) 391, 451, 1443, 1492, 2069, 2754
BveI ACCTGC 2 cut(s) 1255, 1354
Cac8I GCNNGC 9 cut(s) 271, 338, 967, 971, 1246, 2592, 2719, 3283, 3331
CaiI CAGNNNCTG 1 cut(s) 979
CciI TCATGA 2 cut(s) 892, 2155
CfoI GCGC 4 cut(s) 338, 340, 687, 1517
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 6 cut(s) 219, 399, 1527, 2008, 2217, 2547
CseI GACGC 2 cut(s) 170, 1820
Csp6I GTAC 9 cut(s) 215, 235, 652, 1213, 1650, 1685, 1981, 2765, 3485
CviQI GTAC 9 cut(s) 215, 235, 652, 1213, 1650, 1685, 1981, 2765, 3485
DraI TTTAAA 3 cut(s) 544, 2412, 2683
DrdI GACNNNNNNGTC 3 cut(s) 418, 1615, 2961
DriI GACNNNNNGTC 2 cut(s) 16, 352
DseDI GACNNNNNNGTC 3 cut(s) 418, 1615, 2961
EaeI YGGCCR 2 cut(s) 1383, 3058
Eam1104I CTCTTC 7 cut(s) 293, 317, 2331, 2452, 2488, 2565, 2817
Eam1105I GACNNNNNGTC 2 cut(s) 16, 352
EarI CTCTTC 7 cut(s) 293, 317, 2331, 2452, 2488, 2565, 2817
EciI GGCGGA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 1998
Eco130I CCWWGG 4 cut(s) 1273, 2233, 2722, 3335
Eco24I GRGCYC 2 cut(s) 2000, 3176
Eco31I GGTCTC 1 cut(s) 702
Eco32I GATATC 1 cut(s) 2070
Eco47I GGWCC 3 cut(s) 1527, 2217, 2547
Eco53kI GAGCTC 1 cut(s) 1998
Eco57I CTGAAG 2 cut(s) 1764, 1911
Eco88I CYCGRG 2 cut(s) 802, 858
EcoICRI GAGCTC 1 cut(s) 1998
EcoRI GAATTC 1 cut(s) 1412
EcoRII CCWGG 5 cut(s) 1181, 1227, 1820, 2109, 3299
EcoRV GATATC 1 cut(s) 2070
EcoT14I CCWWGG 4 cut(s) 1273, 2233, 2722, 3335
EcoT22I ATGCAT 2 cut(s) 607, 2640
EcoT38I GRGCYC 2 cut(s) 2000, 3176
ErhI CCWWGG 4 cut(s) 1273, 2233, 2722, 3335
Esp3I CGTCTC 1 cut(s) 339
FalI AAGNNNNNCTT 6 cut(s) 1503, 1535, 1929, 1961, 2449, 2481
FaqI GGGAC 3 cut(s) 92, 1930, 2230
FauI CCCGC 3 cut(s) 344, 1306, 3417
FauNDI CATATG 1 cut(s) 3265
FbaI TGATCA 3 cut(s) 1861, 2482, 2782
Fnu4HI GCNGC 8 cut(s) 84, 516, 786, 950, 1242, 2322, 2793, 2811
FriOI GRGCYC 2 cut(s) 2000, 3176
Fsp4HI GCNGC 8 cut(s) 84, 516, 786, 950, 1242, 2322, 2793, 2811
GlaI GCGC 4 cut(s) 337, 339, 686, 1516
GluI GCNGC 8 cut(s) 84, 516, 786, 950, 1242, 2322, 2793, 2811
GsaI CCCAGC 2 cut(s) 226, 2989
GsuI CTGGAG 4 cut(s) 795, 1165, 1275, 2132
HaeII RGCGCY 2 cut(s) 688, 1518
HaeIII GGCC 7 cut(s) 16, 220, 401, 467, 1385, 2009, 3060
HapII CCGG 3 cut(s) 197, 363, 534
HgaI GACGC 2 cut(s) 170, 1820
HhaI GCGC 4 cut(s) 338, 340, 687, 1517
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 4 cut(s) 336, 338, 685, 1515
HinP1I GCGC 4 cut(s) 336, 338, 685, 1515
HincII GTYRAC 1 cut(s) 2829
HindII GTYRAC 1 cut(s) 2829
HindIII AAGCTT 2 cut(s) 546, 1746
HpaI GTTAAC 1 cut(s) 2829
HpaII CCGG 3 cut(s) 197, 363, 534
HphI GGTGA 3 cut(s) 127, 743, 1811
Hpy166II GTNNAC 4 cut(s) 617, 695, 1530, 2829
Hpy8I GTNNAC 4 cut(s) 617, 695, 1530, 2829
Hpy99I CGWCG 9 cut(s) 11, 22, 43, 49, 55, 61, 164, 215, 424
HpyCH4IV ACGT 5 cut(s) 105, 213, 2299, 2767, 3074
HpySE526I ACGT 5 cut(s) 105, 213, 2299, 2767, 3074
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 4 cut(s) 336, 338, 685, 1515
Ksp22I TGATCA 3 cut(s) 1861, 2482, 2782
KspAI GTTAAC 1 cut(s) 2829
LguI GCTCTTC 1 cut(s) 2331
LmnI GCTCC 7 cut(s) 202, 333, 536, 883, 1443, 2560, 2587
Lsp1109I GCAGC 7 cut(s) 502, 772, 961, 1228, 2308, 2779, 2797
LweI GCATC 8 cut(s) 580, 586, 592, 895, 1749, 1860, 2934, 3242
MaeII ACGT 5 cut(s) 105, 213, 2299, 2767, 3074
MaeIII GTNAC 3 cut(s) 795, 2025, 2377
MbiI CCGCTC 1 cut(s) 1391
MfeI CAATTG 1 cut(s) 2705
MflI RGATCY 3 cut(s) 260, 1294, 3406
MhlI GDGCHC 6 cut(s) 967, 1476, 2000, 2395, 2719, 3176
MlsI TGGCCA 2 cut(s) 1385, 3060
MluNI TGGCCA 2 cut(s) 1385, 3060
MlyI GAGTC 5 cut(s) 803, 850, 970, 1962, 2999
MmeI TCCRAC 5 cut(s) 147, 402, 1483, 1518, 1586
Mox20I TGGCCA 2 cut(s) 1385, 3060
Mph1103I ATGCAT 2 cut(s) 607, 2640
MroXI GAANNNNTTC 1 cut(s) 2529
MscI TGGCCA 2 cut(s) 1385, 3060
MseI TTAA 9 cut(s) 543, 680, 867, 1020, 1091, 2165, 2411, 2682, 2828
MslI CAYNNNNRTG 4 cut(s) 891, 1593, 2062, 2942
Msp20I TGGCCA 2 cut(s) 1385, 3060
MspA1I CMGCKG 1 cut(s) 2792
MspCI CTTAAG 1 cut(s) 2164
MspI CCGG 3 cut(s) 197, 363, 534
MspR9I CCNGG 6 cut(s) 535, 1183, 1229, 1822, 2111, 3301
MunI CAATTG 1 cut(s) 2705
Mva1269I GAATGC 2 cut(s) 1123, 1257
MvaI CCWGG 5 cut(s) 1183, 1229, 1822, 2111, 3301
MvnI CGCG 1 cut(s) 338
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 2 cut(s) 2233, 3335
NdeI CATATG 1 cut(s) 3265
NheI GCTAGC 1 cut(s) 2590
NlaIV GGNNCC 3 cut(s) 221, 262, 2218
NmuCI GTSAC 1 cut(s) 795
NsiI ATGCAT 2 cut(s) 607, 2640
NspI RCATGY 4 cut(s) 1163, 1223, 1248, 3285
PaeI GCATGC 2 cut(s) 1248, 3285
PagI TCATGA 2 cut(s) 892, 2155
PauI GCGCGC 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 2331
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 53
PctI GAATGC 2 cut(s) 1123, 1257
PdmI GAANNNNTTC 1 cut(s) 2529
Pfl23II CGTACG 2 cut(s) 214, 234
PflMI CCANNNNNTGG 1 cut(s) 1381
PfoI TCCNGGA 1 cut(s) 533
PkrI GCNGC 8 cut(s) 85, 517, 787, 951, 1243, 2323, 2794, 2812
PleI GAGTC 5 cut(s) 802, 850, 970, 1961, 2998
PpsI GAGTC 5 cut(s) 802, 850, 970, 1961, 2998
Ppu21I YACGTR 2 cut(s) 2300, 2768
PshAI GACNNNNGTC 1 cut(s) 3108
PshBI ATTAAT 1 cut(s) 1091
PsiI TTATAA 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 2000
Psp6I CCWGG 5 cut(s) 1181, 1227, 1820, 2109, 3299
PspFI CCCAGC 2 cut(s) 222, 2985
PspGI CCWGG 5 cut(s) 1181, 1227, 1820, 2109, 3299
PspLI CGTACG 2 cut(s) 214, 234
PspN4I GGNNCC 3 cut(s) 221, 262, 2218
PspPI GGNCC 6 cut(s) 219, 399, 1527, 2008, 2217, 2547
PstI CTGCAG 1 cut(s) 2372
PstNI CAGNNNCTG 1 cut(s) 979
PsuI RGATCY 3 cut(s) 260, 1294, 3406
PteI GCGCGC 1 cut(s) 336
RsaI GTAC 9 cut(s) 216, 236, 653, 1214, 1651, 1686, 1982, 2766, 3486
RsaNI GTAC 9 cut(s) 215, 235, 652, 1213, 1650, 1685, 1981, 2765, 3485
RseI CAYNNNNRTG 4 cut(s) 891, 1593, 2062, 2942
SacI GAGCTC 1 cut(s) 2000
SapI GCTCTTC 1 cut(s) 2331
SaqAI TTAA 9 cut(s) 543, 680, 867, 1020, 1091, 2165, 2411, 2682, 2828
SatI GCNGC 8 cut(s) 84, 516, 786, 950, 1242, 2322, 2793, 2811
Sau96I GGNCC 6 cut(s) 219, 399, 1527, 2008, 2217, 2547
ScaI AGTACT 1 cut(s) 3486
SchI GAGTC 5 cut(s) 803, 850, 970, 1962, 2999
ScrFI CCNGG 6 cut(s) 535, 1183, 1229, 1822, 2111, 3301
SduI GDGCHC 6 cut(s) 967, 1476, 2000, 2395, 2719, 3176
SfaNI GCATC 8 cut(s) 580, 586, 592, 895, 1749, 1860, 2934, 3242
SfcI CTRYAG 2 cut(s) 481, 2368
SinI GGWCC 3 cut(s) 1527, 2217, 2547
SmiMI CAYNNNNRTG 4 cut(s) 891, 1593, 2062, 2942
SmlI CTYRAG 2 cut(s) 1688, 2164
SmoI CTYRAG 2 cut(s) 1688, 2164
SphI GCATGC 2 cut(s) 1248, 3285
SspI AATATT 1 cut(s) 2479
SstI GAGCTC 1 cut(s) 2000
StyD4I CCNGG 6 cut(s) 533, 1181, 1227, 1820, 2109, 3299
StyI CCWWGG 4 cut(s) 1273, 2233, 2722, 3335
TaiI ACGT 5 cut(s) 108, 216, 2302, 2770, 3077
TaqI TCGA 4 cut(s) 374, 499, 822, 1949
TatI WGTACW 3 cut(s) 651, 1684, 3484
TauI GCSGC 1 cut(s) 86
Tru1I TTAA 9 cut(s) 543, 680, 867, 1020, 1091, 2165, 2411, 2682, 2828
Tru9I TTAA 9 cut(s) 543, 680, 867, 1020, 1091, 2165, 2411, 2682, 2828
TscAI CASTG 6 cut(s) 391, 451, 1450, 1492, 2069, 2754
TseFI GTSAC 1 cut(s) 795
TseI GCWGC 7 cut(s) 515, 785, 949, 1241, 2321, 2792, 2810
Tsp45I GTSAC 1 cut(s) 795
TspGWI ACGGA 2 cut(s) 2667, 3279
TspRI CASTG 6 cut(s) 391, 451, 1450, 1492, 2069, 2754
Van91I CCANNNNNTGG 1 cut(s) 1381
Vha464I CTTAAG 1 cut(s) 2164
VpaK11BI GGWCC 3 cut(s) 1527, 2217, 2547
VspI ATTAAT 1 cut(s) 1091
XapI RAATTY 7 cut(s) 538, 1412, 1694, 2698, 2918, 3306, 3462
XbaI TCTAGA 1 cut(s) 2860
XceI RCATGY 4 cut(s) 1163, 1223, 1248, 3285
XmnI GAANNNNTTC 1 cut(s) 2529
ZrmI AGTACT 1 cut(s) 3486
Zsp2I ATGCAT 2 cut(s) 607, 2640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.