MD15G1053400.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002261
Physical Location & Seq
Forward (+)
3656702 .. 3658845
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1053400.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGAAGCTGCTGAGTTTGTTGGAGGAACAGAGAGAAGAAGAAGCAAAACAGAGAGAGAAGGTCATGCAGAAAGAGCTCCATCGAGCCGCCGTGCGGGGCTCCGTCGACGCCCTCCTCGAACTCCTCACAGAAGACCCTCAAATCCTCAGTAAGCCCGCCCCATCTCTCTCCGACACCCCCTTACACGTCGTCTCGCTTCTGGGTCACTCTGCTTTCGCCAAAGAGTTACTGAGTCGGAATCCCGAACTCGCCGCCCAGTTGGATTCCCGCGGCTCTTCCCCTCTTCACCTGGCGGCGGCAAAAGGGTCGGTGGAGATAGTGAAAAGTCTGGTGCTGGTCAACCCAGATCTGGGTTTGGTCTGGGATCGCGATGGATTCACTCCCCTGCATCTGGGTGTGATCAAGGGGCGAGCTGCGGTGGTGGCTGAGTTGGCTCAAGTCATACCGGCGGCGACTCGGGTTTTGACTCAGGGAGGCGAGAGTGGGCTGCACTTGTGTGTGAAGCATCACAGGCTGGAGGTATTGAAGGTTTTGGTGGAATGTATTGGGCGAGGTGATGAGTTTGTGAATTGGAGGGATGGCGATGGCAACACAGTCTTACATGTTGCTGTGGCCAAGAAACAATTAGAGGTTATAAAATATTTGCTGACTAATACAAAGATAGATGTAAAAGCCCAGAATGCGAATGGTTTCACTGCATTGGACGTCTTATCTCATAGCACTAGAGATTTAAGAGACTTGGAAATCAAACAGTGTCTTCAGAAAGCTGCAGCTCCAGGAATCAACAAGTCACGATCCGTTGCATACGAGCCGGAAATGGATTCGGTTCGAGTCTCCAACTCGATCTCAATGCACCCACTAATGTCGAAGAAAATGAGTATAAAGGAAATAGTAAAGAAGAAGGACATTGATTGGTTGGGAAGGAAACGAAGCTCGTTAATGGTTGTGACTTCCCTAATTGCGACAGTGGCATTTCAAGCTGCATTATCCCCGCCAGGCGGTGTATGGCAGGATGACTATTTCGAATACTCCAATGGCACGGCCGTGGAGCGTCCGCACACAGCCGGGCAGTCAGTGATGGCAAATACAGAGCCCCGTAATTATGGACAGTTCATGATCTTCAACACGATTGCTTTCCTTGCATCATTGAGCATAATTCTACTGCTGGTGAGCGGATTGCCTATGAGGCGGAAGAGATTGATGTGGCTTCAGATGGTGATCATGTGGATTGCAATAACGGCACTAACGGGTACCTATTTTATCGGATTAATCATCATGACACCGAATAAGAGAAAGGGTAGCTATCTGTATTATGTGACACAAGTTTCTGTGCTGATATGGCTGGCGTTGATGGGGATCGTGTTCATTGGTAACGTGATTCGGGTAATCATATGGCTTCTTAGAAAGTATGGATACATGGAGCAGAAAGAAACAGATGATTCACTCTACGAAGACTACGAAGACAATGACGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

493

Amino Acids

54.81

Weight (kDa)

8.5

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 26 - 111 2.2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 88 - 145 2e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 152 - 226 1.6e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 163 - 216 5.9e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 189 - 236 2.8e-07 Ankyrin repeats (many copies)
PGG PF13962 304 - 425 5.8e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AatII GACGTC 1 cut(s) 708
Acc65I GGTACC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 1250
AccBSI CCGCTC 1 cut(s) 1173
AccI GTMKAC 1 cut(s) 105
AccII CGCG 2 cut(s) 270, 369
AclWI GGATC 3 cut(s) 372, 789, 1364
AcoI YGGCCR 2 cut(s) 612, 1041
AcsI RAATTY 1 cut(s) 1473
AcuI CTGAAG 2 cut(s) 743, 1193
AcyI GRCGYC 2 cut(s) 108, 705
AfaI GTAC 1 cut(s) 1252
AfiI CCNNNNNNNGG 6 cut(s) 93, 295, 349, 350, 391, 998
AflIII ACRYGT 2 cut(s) 184, 601
AgsI TTSAA 3 cut(s) 526, 977, 1123
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 3 cut(s) 288, 775, 994
AleI CACNNNNGTG 2 cut(s) 495, 1043
AluBI AGCT 8 cut(s) 7, 76, 413, 767, 773, 933, 980, 1302
AluI AGCT 8 cut(s) 7, 76, 413, 767, 773, 933, 980, 1302
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 3 cut(s) 196, 729, 838
AlwI GGATC 3 cut(s) 372, 789, 1364
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 2 cut(s) 612, 1041
ApeKI GCWGC 6 cut(s) 7, 413, 487, 767, 770, 980
ApoI RAATTY 1 cut(s) 1473
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
AseI ATTAAT 1 cut(s) 1268
Asp700I GAANNNNTTC 1 cut(s) 689
Asp718I GGTACC 1 cut(s) 1250
AsuC2I CCSGG 1 cut(s) 1066
AsuHPI GGTGA 4 cut(s) 278, 566, 1180, 1228
AsuII TTCGAA 1 cut(s) 1023
AvaI CYCGRG 1 cut(s) 456
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 1250
BanII GRGCYC 3 cut(s) 78, 101, 1095
BbsI GAAGAC 4 cut(s) 138, 749, 1458, 1467
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 5 cut(s) 400, 474, 754, 782, 967
BccI CCATC 8 cut(s) 87, 169, 365, 572, 578, 1072, 1207, 1345
BceAI ACGGC 4 cut(s) 74, 1028, 1056, 1254
BcgI CGANNNNNNTGC 4 cut(s) 288, 322, 832, 866
BciT130I CCWGG 3 cut(s) 290, 777, 996
BciVI GTATCC 1 cut(s) 1406
BclI TGATCA 2 cut(s) 399, 1218
BcnI CCSGG 1 cut(s) 1066
BcoDI GTCTC 3 cut(s) 196, 729, 838
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 1 cut(s) 768
BfuI GTATCC 1 cut(s) 1406
BglI GCCNNNNNGGC 1 cut(s) 1186
BglII AGATCT 1 cut(s) 346
Bme1390I CCNGG 4 cut(s) 290, 777, 996, 1066
BmeT110I CYCGRG 1 cut(s) 456
BmgBI CACGTC 1 cut(s) 187
BmiI GGNNCC 2 cut(s) 100, 1252
BmrFI CCNGG 4 cut(s) 290, 777, 996, 1066
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 397, 514, 1151
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 4 cut(s) 138, 749, 1458, 1467
BpmI CTGGAG 2 cut(s) 536, 759
Bpu14I TTCGAA 1 cut(s) 1023
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 1066
BsaBI GATNNNNATC 2 cut(s) 1217, 1355
BsaHI GRCGYC 2 cut(s) 108, 705
BsaJI CCNNGG 2 cut(s) 268, 1044
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 295, 349, 350, 391, 998
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 1 cut(s) 256
Bse8I GATNNNNATC 2 cut(s) 1217, 1355
BseBI CCWGG 3 cut(s) 290, 777, 996
BseDI CCNNGG 2 cut(s) 268, 1044
BseGI GGATG 2 cut(s) 583, 1018
BseJI GATNNNNATC 2 cut(s) 1217, 1355
BseLI CCNNNNNNNGG 6 cut(s) 93, 295, 349, 350, 391, 998
BseMII CTCAG 4 cut(s) 160, 221, 417, 482
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 104, 113
BseX3I CGGCCG 1 cut(s) 1041
BseXI GCAGC 5 cut(s) 400, 474, 754, 782, 967
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 2 cut(s) 270, 369
Bsh1285I CGRYCG 1 cut(s) 1044
BshFI GGCC 2 cut(s) 614, 1043
BshNI GGYRCC 1 cut(s) 1250
BsiEI CGRYCG 1 cut(s) 1044
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 3 cut(s) 446, 812, 1065
BslI CCNNNNNNNGG 6 cut(s) 93, 295, 349, 350, 391, 998
BsmAI GTCTC 3 cut(s) 196, 729, 838
BsmBI CGTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 2 cut(s) 614, 1043
BsoBI CYCGRG 1 cut(s) 456
Bsp119I TTCGAA 1 cut(s) 1023
Bsp1286I GDGCHC 3 cut(s) 78, 101, 1095
Bsp143I GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
Bsp68I TCGCGA 1 cut(s) 369
BspANI GGCC 2 cut(s) 614, 1043
BspCNI CTCAG 4 cut(s) 159, 222, 418, 481
BspFNI CGCG 2 cut(s) 270, 369
BspHI TCATGA 2 cut(s) 1113, 1275
BspLI GGNNCC 2 cut(s) 100, 1252
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 3 cut(s) 372, 789, 1364
BspQI GCTCTTC 1 cut(s) 280
BspT104I TTCGAA 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 1250
BsrBI CCGCTC 1 cut(s) 1173
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 268, 1044
BssMI GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
BssNI GRCGYC 2 cut(s) 108, 705
Bst2UI CCWGG 3 cut(s) 290, 777, 996
Bst4CI ACNGT 4 cut(s) 595, 753, 967, 1110
Bst6I CTCTTC 3 cut(s) 280, 288, 1187
BstACI GRCGYC 2 cut(s) 108, 705
BstBI TTCGAA 1 cut(s) 1023
BstC8I GCNNGC 3 cut(s) 156, 411, 1344
BstDEI CTNAG 6 cut(s) 11, 146, 230, 426, 468, 1400
BstDSI CCRYGG 2 cut(s) 268, 1044
BstF5I GGATG 2 cut(s) 583, 1018
BstFNI CGCG 2 cut(s) 270, 369
BstKTI GATC 8 cut(s) 349, 367, 402, 797, 846, 1119, 1221, 1359
BstMAI GTCTC 3 cut(s) 196, 729, 838
BstMBI GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
BstMCI CGRYCG 1 cut(s) 1044
BstNI CCWGG 3 cut(s) 290, 777, 996
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 4 cut(s) 288, 775, 994, 1064
BstSFI CTRYAG 1 cut(s) 768
BstUI CGCG 2 cut(s) 270, 369
BstV1I GCAGC 5 cut(s) 400, 474, 754, 782, 967
BstV2I GAAGAC 4 cut(s) 138, 749, 1458, 1467
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 1041
BsuI GTATCC 1 cut(s) 1406
BsuRI GGCC 2 cut(s) 614, 1043
BtgI CCRYGG 2 cut(s) 268, 1044
BtgZI GCGATG 2 cut(s) 384, 597
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 2 cut(s) 583, 1018
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 4 cut(s) 693, 758, 972, 1080
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 156, 411, 1344
CciI TCATGA 2 cut(s) 1113, 1275
Cfr10I RCCGGY 1 cut(s) 445
Cfr42I CCGCGG 1 cut(s) 271
CseI GACGC 2 cut(s) 116, 1040
Csp6I GTAC 1 cut(s) 1251
CviAII CATG 6 cut(s) 64, 602, 1114, 1222, 1276, 1417
CviQI GTAC 1 cut(s) 1251
DdeI CTNAG 6 cut(s) 11, 146, 230, 426, 468, 1400
DpnI GATC 8 cut(s) 348, 366, 401, 796, 845, 1118, 1220, 1358
DpnII GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
EaeI YGGCCR 2 cut(s) 612, 1041
EagI CGGCCG 1 cut(s) 1041
Eam1104I CTCTTC 3 cut(s) 280, 288, 1187
EarI CTCTTC 3 cut(s) 280, 288, 1187
EciI GGCGGA 1 cut(s) 1204
Ecl136II GAGCTC 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 1041
Eco24I GRGCYC 3 cut(s) 78, 101, 1095
Eco52I CGGCCG 1 cut(s) 1041
Eco53kI GAGCTC 1 cut(s) 76
Eco57I CTGAAG 2 cut(s) 743, 1193
Eco88I CYCGRG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 288, 775, 994
EcoT38I GRGCYC 3 cut(s) 78, 101, 1095
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 6 cut(s) 67, 605, 1117, 1225, 1279, 1420
FatI CATG 6 cut(s) 63, 601, 1113, 1221, 1275, 1416
FauI CCCGC 4 cut(s) 87, 163, 275, 999
FauNDI CATATG 1 cut(s) 1391
FbaI TGATCA 2 cut(s) 399, 1218
FblI GTMKAC 1 cut(s) 105
FokI GGATG 2 cut(s) 590, 1025
FriOI GRGCYC 3 cut(s) 78, 101, 1095
FspBI CTAG 1 cut(s) 723
GsuI CTGGAG 2 cut(s) 536, 759
HaeIII GGCC 2 cut(s) 614, 1043
HapII CCGG 3 cut(s) 446, 812, 1065
HgaI GACGC 2 cut(s) 116, 1040
Hin1I GRCGYC 2 cut(s) 108, 705
Hin1II CATG 6 cut(s) 67, 605, 1117, 1225, 1279, 1420
HincII GTYRAC 2 cut(s) 106, 340
HindII GTYRAC 2 cut(s) 106, 340
HpaII CCGG 3 cut(s) 446, 812, 1065
HphI GGTGA 4 cut(s) 278, 566, 1180, 1228
Hpy166II GTNNAC 2 cut(s) 106, 340
Hpy188I TCNGA 5 cut(s) 172, 237, 762, 1212, 1265
Hpy188III TCNNGA 5 cut(s) 242, 368, 792, 1114, 1276
Hpy8I GTNNAC 2 cut(s) 106, 340
Hpy99I CGWCG 3 cut(s) 107, 110, 191
HpyAV CCTTC 4 cut(s) 52, 520, 895, 915
HpyCH4III ACNGT 4 cut(s) 595, 753, 967, 1110
HpyCH4IV ACGT 3 cut(s) 186, 705, 1374
HpyF3I CTNAG 6 cut(s) 11, 146, 230, 426, 468, 1400
HpySE526I ACGT 3 cut(s) 186, 705, 1374
Hsp92I GRCGYC 2 cut(s) 108, 705
Hsp92II CATG 6 cut(s) 67, 605, 1117, 1225, 1279, 1420
KpnI GGTACC 1 cut(s) 1254
Ksp22I TGATCA 2 cut(s) 399, 1218
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
LguI GCTCTTC 1 cut(s) 280
LmnI GCTCC 5 cut(s) 81, 104, 778, 1048, 1420
Lsp1109I GCAGC 5 cut(s) 400, 474, 754, 782, 967
LweI GCATC 3 cut(s) 397, 514, 1151
MaeI CTAG 1 cut(s) 723
MaeII ACGT 3 cut(s) 186, 705, 1374
MaeIII GTNAC 6 cut(s) 203, 225, 789, 946, 1315, 1370
MalI GATC 8 cut(s) 348, 366, 401, 796, 845, 1118, 1220, 1358
MbiI CCGCTC 1 cut(s) 1173
MboI GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 3 cut(s) 78, 101, 1095
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 6 cut(s) 568, 623, 957, 1099, 1155, 1473
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 4 cut(s) 241, 448, 460, 840
MmeI TCCRAC 4 cut(s) 195, 215, 240, 861
Mox20I TGGCCA 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 689
MscI TGGCCA 1 cut(s) 614
MseI TTAA 3 cut(s) 731, 938, 1268
MslI CAYNNNNRTG 3 cut(s) 393, 495, 1043
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 3 cut(s) 446, 812, 1065
MspR9I CCNGG 4 cut(s) 290, 777, 996, 1066
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 3 cut(s) 290, 777, 996
MvnI CGCG 2 cut(s) 270, 369
NciI CCSGG 1 cut(s) 1066
NdeI CATATG 1 cut(s) 1391
NdeII GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
NlaIII CATG 6 cut(s) 67, 605, 1117, 1225, 1279, 1420
NlaIV GGNNCC 2 cut(s) 100, 1252
NmuCI GTSAC 4 cut(s) 203, 789, 946, 1315
NruI TCGCGA 1 cut(s) 369
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 1023
OliI CACNNNNGTG 2 cut(s) 495, 1043
PagI TCATGA 2 cut(s) 1113, 1275
PciI ACATGT 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 280
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 1455
PctI GAATGC 1 cut(s) 685
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 7 cut(s) 238, 263, 375, 780, 821, 1378, 1439
PfoI TCCNGGA 1 cut(s) 775
PleI GAGTC 4 cut(s) 240, 448, 460, 839
PpsI GAGTC 4 cut(s) 240, 448, 460, 839
PscI ACATGT 1 cut(s) 601
PshBI ATTAAT 1 cut(s) 1268
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 288, 775, 994
PspGI CCWGG 3 cut(s) 288, 775, 994
PspN4I GGNNCC 2 cut(s) 100, 1252
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 1 cut(s) 346
RruI TCGCGA 1 cut(s) 369
RsaI GTAC 1 cut(s) 1252
RsaNI GTAC 1 cut(s) 1251
RseI CAYNNNNRTG 3 cut(s) 393, 495, 1043
SacI GAGCTC 1 cut(s) 78
SacII CCGCGG 1 cut(s) 271
SalI GTCGAC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 280
SaqAI TTAA 3 cut(s) 731, 938, 1268
Sau3AI GATC 8 cut(s) 346, 364, 399, 794, 843, 1116, 1218, 1356
SchI GAGTC 4 cut(s) 241, 448, 460, 840
ScrFI CCNGG 4 cut(s) 290, 777, 996, 1066
SduI GDGCHC 3 cut(s) 78, 101, 1095
SfaNI GCATC 3 cut(s) 397, 514, 1151
SfcI CTRYAG 1 cut(s) 768
Sfr303I CCGCGG 1 cut(s) 271
SfuI TTCGAA 1 cut(s) 1023
SgrBI CCGCGG 1 cut(s) 271
SgrDI CGTCGACG 1 cut(s) 104
SmiMI CAYNNNNRTG 3 cut(s) 393, 495, 1043
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 6 cut(s) 568, 623, 957, 1099, 1155, 1473
SspI AATATT 1 cut(s) 641
SspMI CTAG 1 cut(s) 723
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 4 cut(s) 288, 775, 994, 1064
TaaI ACNGT 4 cut(s) 595, 753, 967, 1110
TaiI ACGT 3 cut(s) 189, 708, 1377
TaqI TCGA 7 cut(s) 82, 105, 117, 829, 842, 866, 1023
TasI AATT 6 cut(s) 568, 623, 957, 1099, 1155, 1473
TauI GCSGC 6 cut(s) 89, 254, 273, 296, 299, 452
TfiI GAWTC 7 cut(s) 238, 263, 375, 780, 821, 1378, 1439
Tru1I TTAA 3 cut(s) 731, 938, 1268
Tru9I TTAA 3 cut(s) 731, 938, 1268
TscAI CASTG 4 cut(s) 700, 758, 972, 1080
TseFI GTSAC 4 cut(s) 203, 789, 946, 1315
TseI GCWGC 6 cut(s) 7, 413, 487, 767, 770, 980
Tsp45I GTSAC 4 cut(s) 203, 789, 946, 1315
TspDTI ATGAA 3 cut(s) 17, 1102, 1354
TspGWI ACGGA 2 cut(s) 91, 787
TspRI CASTG 4 cut(s) 700, 758, 972, 1080
VspI ATTAAT 1 cut(s) 1268
XapI RAATTY 1 cut(s) 1473
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 2 cut(s) 683, 1002
XmiI GTMKAC 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 1 cut(s) 723
ZraI GACGTC 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.