pycom15g18950

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002261
Physical Location & Seq
Reverse (-)
13436840 .. 13438733
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g18950.1

Sequence Viewer

Length: 1422 bp
ATGGACAAGAAGCTTCTGGATGCAGCCATGGCGGGAGACACTGCGGCGCTTAAAGATCTGATCGCAGAAGACCCGCTTGTTCTCGACAGAGCTTTAGTCTCCTGCGTCTCCGAGACGCCGCTTCACGTAGCTTCAATGCTAGGTCACCTGGCTTTTGTTACCGAGTTACTGAGCCGCAACCCCGAGCTGGCCTCCGAGCTGGACTCGCATGGGTCAACGCCTCTCCACGTGGCGGCGGCGAAGGGGCACGCAGAGATTGTGAAAGAGCTGGTTCTGGCGGATCCGGGGGCGTGTGCGGTGAGGAACGGAGACGGGAGGACGGCGCTCCACGTAGCGGCCGCCAAGGGGCGGGTTAAAGTAGTGAGAGAGTTGGTCCGAGTTGGGAGTGAGTCGACTCGGGCGTTGACGGACCGAGGCGAGACGGCGCTGCACCTGTGCGTCGGGTGTAACCGGTTGGAGGGGTTGAAGGTTTTGGTCGAGGCAGCTGGGAGTGATGATGATTTTGTGAATTGGAAGGATTGCGACGGTAACACTGTTTTGCATATTGCCGTGGCCAAGAAGCAAACCGAGATTACCAAATTCTTGCTGTCTAAAACCGGAGTGAATGTGAACGCGTTGAATGCAAACAGCGCTACCGCCTTAGACATTCTGATGCAGAGTCCTAGAGATTTGAGGGATATGGAGATTGAGGATTCTCTTCGAGGTGCTGGGGCTCAGAGTGCAAAGGATGTATGTAACATTGTGCACGACTGGGTTCGTGCTCCAACTAAGGAACGTCAGATATCAAGACATCGAGGCTCAGCACTGTCCTCGAGAGTGAGTGTTTCTATGAAACCGGCTGGGAACAAGAAGCCTACTGATTGGCTTGGTAGGAAGAGAAGTTCATTGATGGTGGTGGCATCTCTCCTTGCAACTGTTGCCTTCCAAGCTGCAATAACCCCGCCAGGAGGTTTTTGGCAGGATGACCTGCAAGTTGATGAAAATGGCAACCCTGTGGCGAATCCTCACACCGTAGGAACATCTGTCATGGCAACTAAGCAGGGAAAAGAGTATGGTCTATTCATGATTTTTAACACAATCTCTTTCCTCACATCCCTAAGCATAATTCTTCTGCTTGTTAGCGGGCTACCTATCAAACGGAGGAGATGGATGTGGATACAAATGGTCATAATGTGGGTTGCTGTCACAACACTAGTAATAACTTATTTCATCACTCTCCGTCATATGTCACCTAATGACGTACATGTGCAAATCCTGTTACGTAAGGTAACCGAGATATCAGTTTTGACATGGTTGACTTTAATGGTGGTTGTTTTCTTTGGCAATGTTATTCGTATGAATCTATGGATTCTCAGAAAGTATGGTTATATTAAGCCAAAAGAGGTAGACGATGAACTTGAAGAGGTGAGAACTGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

474

Amino Acids

51.85

Weight (kDa)

7.7

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 91 1.4e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 61 - 112 4.9e-09 Ankyrin repeats (many copies)
Ank PF00023 70 - 97 6.1e-06 Ankyrin repeat
Ank_4 PF13637 75 - 122 5.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 100 - 163 3.9e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 169 - 216 7.1e-06 Ankyrin repeats (many copies)
Ank PF00023 175 - 207 1.8e-06 Ankyrin repeat
PGG PF13962 287 - 406 3.5e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 975
AccI GTMKAC 2 cut(s) 392, 1386
AccII CGCG 1 cut(s) 614
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 2 cut(s) 336, 552
AcsI RAATTY 1 cut(s) 578
AcvI CACGTG 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 1242
AfeI AGCGCT 1 cut(s) 631
AfiI CCNNNNNNNGG 7 cut(s) 187, 232, 334, 345, 348, 457, 947
AflIII ACRYGT 2 cut(s) 612, 1243
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 4 cut(s) 135, 466, 619, 1400
AhlI ACTAGT 1 cut(s) 1192
AjnI CCWGG 2 cut(s) 147, 943
AloI GAACNNNNNNTCC 2 cut(s) 865, 897
AluBI AGCT 8 cut(s) 13, 92, 131, 187, 199, 268, 485, 929
AluI AGCT 8 cut(s) 13, 92, 131, 187, 199, 268, 485, 929
Alw21I GWGCWC 2 cut(s) 747, 763
Alw26I GTCTC 6 cut(s) 30, 103, 107, 112, 303, 413
Alw44I GTGCAC 1 cut(s) 743
AlwI GGATC 2 cut(s) 275, 288
Ama87I CYCGRG 3 cut(s) 182, 396, 811
Aor51HI AGCGCT 1 cut(s) 631
AoxI GGCC 3 cut(s) 189, 336, 552
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 4 cut(s) 23, 427, 482, 929
ApoI RAATTY 1 cut(s) 578
AsiGI ACCGGT 1 cut(s) 450
AspLEI GCGC 4 cut(s) 49, 325, 427, 632
AspS9I GGNCC 2 cut(s) 373, 409
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 4 cut(s) 137, 310, 1221, 1417
AvaI CYCGRG 3 cut(s) 182, 396, 811
AvaII GGWCC 2 cut(s) 373, 409
BaeGI GKGCMC 2 cut(s) 249, 747
BalI TGGCCA 1 cut(s) 554
BamHI GGATCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 715
BbrPI CACGTG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 747, 763
BbvI GCAGC 4 cut(s) 35, 414, 494, 916
BccI CCATC 2 cut(s) 883, 1140
BceAI ACGGC 3 cut(s) 336, 438, 533
BcgI CGANNNNNNTGC 2 cut(s) 792, 826
BciT130I CCWGG 2 cut(s) 149, 945
BciVI GTATCC 1 cut(s) 1149
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 6 cut(s) 30, 103, 107, 112, 303, 413
BcuI ACTAGT 1 cut(s) 1192
BfaI CTAG 3 cut(s) 140, 663, 1193
BfoI RGCGCY 4 cut(s) 50, 326, 428, 633
BfuAI ACCTGC 1 cut(s) 975
BfuI GTATCC 1 cut(s) 1149
BglII AGATCT 1 cut(s) 55
BlpI GCTNAGC 1 cut(s) 799
Bme1390I CCNGG 3 cut(s) 149, 285, 945
Bme18I GGWCC 2 cut(s) 373, 409
BmeT110I CYCGRG 3 cut(s) 182, 396, 811
BmgT120I GGNCC 2 cut(s) 373, 409
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 3 cut(s) 149, 285, 945
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 3 cut(s) 10, 642, 908
BmuI ACTGGG 1 cut(s) 760
BpiI GAAGAC 1 cut(s) 75
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 188, 220
Bpu10I CCTNAGC 1 cut(s) 1097
Bpu1102I GCTNAGC 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 285
BsaAI YACGTR 4 cut(s) 127, 229, 331, 1262
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 5 cut(s) 27, 284, 342, 412, 549
BsaWI WCCGGW 2 cut(s) 450, 596
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 232, 334, 345, 348, 457, 947
Bse118I RCCGGY 2 cut(s) 450, 835
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 1 cut(s) 1330
BseBI CCWGG 2 cut(s) 149, 945
BseDI CCNNGG 5 cut(s) 27, 284, 342, 412, 549
BseGI GGATG 5 cut(s) 25, 733, 967, 1091, 1155
BseLI CCNNNNNNNGG 7 cut(s) 187, 232, 334, 345, 348, 457, 947
BseMI GCAATG 1 cut(s) 1330
BseMII CTCAG 5 cut(s) 161, 728, 813, 1366, 1404
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 1 cut(s) 1156
BseSI GKGCMC 2 cut(s) 249, 747
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 4 cut(s) 35, 414, 494, 916
BseYI CCCAGC 3 cut(s) 485, 707, 839
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 1 cut(s) 614
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 191, 338, 554
BshTI ACCGGT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 2 cut(s) 747, 763
BsiHKCI CYCGRG 3 cut(s) 182, 396, 811
BsiSI CCGG 4 cut(s) 284, 451, 597, 836
BslI CCNNNNNNNGG 7 cut(s) 187, 232, 334, 345, 348, 457, 947
BsmAI GTCTC 6 cut(s) 30, 103, 107, 112, 303, 413
BsmBI CGTCTC 4 cut(s) 107, 112, 303, 413
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 3 cut(s) 191, 338, 554
BsoBI CYCGRG 3 cut(s) 182, 396, 811
Bsp1286I GDGCHC 4 cut(s) 249, 715, 747, 763
Bsp143I GATC 3 cut(s) 55, 60, 280
Bsp1720I GCTNAGC 1 cut(s) 799
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 3 cut(s) 191, 338, 554
BspCNI CTCAG 5 cut(s) 162, 727, 812, 1365, 1405
BspFNI CGCG 1 cut(s) 614
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 975
BspPI GGATC 2 cut(s) 275, 288
BsrDI GCAATG 1 cut(s) 1330
BsrFI RCCGGY 2 cut(s) 450, 835
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 2 cut(s) 450, 835
BssECI CCNNGG 5 cut(s) 27, 284, 342, 412, 549
BssMI GATC 3 cut(s) 55, 60, 280
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 27, 342
Bst2UI CCWGG 2 cut(s) 149, 945
Bst4CI ACNGT 5 cut(s) 527, 535, 807, 916, 1012
Bst6I CTCTTC 3 cut(s) 702, 869, 1395
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 917
BstBAI YACGTR 4 cut(s) 127, 229, 331, 1262
BstC8I GCNNGC 3 cut(s) 189, 249, 1124
BstDEI CTNAG 9 cut(s) 170, 640, 714, 768, 799, 1035, 1097, 1352, 1413
BstDSI CCRYGG 2 cut(s) 27, 549
BstEII GGTNACC 2 cut(s) 143, 1267
BstF5I GGATG 5 cut(s) 25, 733, 967, 1091, 1155
BstFNI CGCG 1 cut(s) 614
BstH2I RGCGCY 4 cut(s) 50, 326, 428, 633
BstHHI GCGC 4 cut(s) 49, 325, 427, 632
BstKTI GATC 3 cut(s) 58, 63, 283
BstMAI GTCTC 6 cut(s) 30, 103, 107, 112, 303, 413
BstMBI GATC 3 cut(s) 55, 60, 280
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 7 cut(s) 29, 205, 620, 629, 719, 917, 926
BstNI CCWGG 2 cut(s) 149, 945
BstNSI RCATGY 1 cut(s) 1247
BstPI GGTNACC 2 cut(s) 143, 1267
BstSCI CCNGG 3 cut(s) 147, 283, 943
BstSLI GKGCMC 2 cut(s) 249, 747
BstSNI TACGTA 1 cut(s) 1262
BstUI CGCG 1 cut(s) 614
BstV1I GCAGC 4 cut(s) 35, 414, 494, 916
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 55, 280
BstYI RGATCY 2 cut(s) 55, 280
BstZI CGGCCG 1 cut(s) 336
BsuI GTATCC 1 cut(s) 1149
BsuRI GGCC 3 cut(s) 191, 338, 554
BtgI CCRYGG 2 cut(s) 27, 549
BtsCI GGATG 5 cut(s) 25, 733, 967, 1091, 1155
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 39, 531, 803
BveI ACCTGC 1 cut(s) 975
Cac8I GCNNGC 3 cut(s) 189, 249, 1124
CciI TCATGA 1 cut(s) 1062
CciNI GCGGCCGC 1 cut(s) 336
CfoI GCGC 4 cut(s) 49, 325, 427, 632
Cfr10I RCCGGY 2 cut(s) 450, 835
Cfr13I GGNCC 2 cut(s) 373, 409
CpoI CGGWCCG 1 cut(s) 409
CseI GACGC 3 cut(s) 94, 124, 427
Csp6I GTAC 1 cut(s) 1241
CspAI ACCGGT 1 cut(s) 450
CspI CGGWCCG 1 cut(s) 409
CviAII CATG 6 cut(s) 28, 209, 1027, 1063, 1244, 1290
CviQI GTAC 1 cut(s) 1241
DdeI CTNAG 9 cut(s) 170, 640, 714, 768, 799, 1035, 1097, 1352, 1413
DpnI GATC 3 cut(s) 57, 62, 282
DpnII GATC 3 cut(s) 55, 60, 280
EaeI YGGCCR 2 cut(s) 336, 552
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 702, 869, 1395
EarI CTCTTC 3 cut(s) 702, 869, 1395
EciI GGCGGA 1 cut(s) 293
EclXI CGGCCG 1 cut(s) 336
Eco105I TACGTA 1 cut(s) 1262
Eco130I CCWWGG 2 cut(s) 27, 342
Eco24I GRGCYC 1 cut(s) 715
Eco32I GATATC 2 cut(s) 783, 1278
Eco47I GGWCC 2 cut(s) 373, 409
Eco47III AGCGCT 1 cut(s) 631
Eco52I CGGCCG 1 cut(s) 336
Eco72I CACGTG 1 cut(s) 229
Eco88I CYCGRG 3 cut(s) 182, 396, 811
Eco91I GGTNACC 2 cut(s) 143, 1267
EcoO65I GGTNACC 2 cut(s) 143, 1267
EcoRII CCWGG 2 cut(s) 147, 943
EcoRV GATATC 2 cut(s) 783, 1278
EcoT14I CCWWGG 2 cut(s) 27, 342
EcoT38I GRGCYC 1 cut(s) 715
ErhI CCWWGG 2 cut(s) 27, 342
Esp3I CGTCTC 4 cut(s) 107, 112, 303, 413
FaeI CATG 6 cut(s) 31, 212, 1030, 1066, 1247, 1293
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 6 cut(s) 27, 208, 1026, 1062, 1243, 1289
FauI CCCGC 5 cut(s) 25, 81, 342, 948, 1115
FauNDI CATATG 1 cut(s) 1224
FblI GTMKAC 2 cut(s) 392, 1386
FokI GGATG 5 cut(s) 32, 740, 974, 1078, 1162
FriOI GRGCYC 1 cut(s) 715
FspBI CTAG 3 cut(s) 140, 663, 1193
GlaI GCGC 4 cut(s) 48, 324, 426, 631
GsaI CCCAGC 3 cut(s) 489, 711, 843
HaeII RGCGCY 4 cut(s) 50, 326, 428, 633
HaeIII GGCC 3 cut(s) 191, 338, 554
HapII CCGG 4 cut(s) 284, 451, 597, 836
HgaI GACGC 3 cut(s) 94, 124, 427
HhaI GCGC 4 cut(s) 49, 325, 427, 632
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 6 cut(s) 31, 212, 1030, 1066, 1247, 1293
Hin6I GCGC 4 cut(s) 47, 323, 425, 630
HinP1I GCGC 4 cut(s) 47, 323, 425, 630
HincII GTYRAC 4 cut(s) 216, 393, 405, 1296
HindII GTYRAC 4 cut(s) 216, 393, 405, 1296
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 8 cut(s) 203, 389, 394, 658, 692, 1000, 1339, 1348
HpaII CCGG 4 cut(s) 284, 451, 597, 836
HphI GGTGA 4 cut(s) 137, 310, 1221, 1417
Hpy166II GTNNAC 7 cut(s) 216, 393, 405, 610, 745, 1296, 1387
Hpy188I TCNGA 8 cut(s) 60, 112, 196, 377, 651, 717, 780, 1355
Hpy188III TCNNGA 5 cut(s) 17, 83, 786, 813, 1063
Hpy8I GTNNAC 7 cut(s) 216, 393, 405, 610, 745, 1296, 1387
Hpy99I CGWCG 2 cut(s) 443, 527
HpyAV CCTTC 4 cut(s) 235, 460, 508, 931
HpyCH4III ACNGT 5 cut(s) 527, 535, 807, 916, 1012
HpyCH4IV ACGT 6 cut(s) 126, 228, 330, 775, 1239, 1261
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 205, 620, 629, 719, 917, 926
HpyF3I CTNAG 9 cut(s) 170, 640, 714, 768, 799, 1035, 1097, 1352, 1413
HpySE526I ACGT 6 cut(s) 126, 228, 330, 775, 1239, 1261
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 6 cut(s) 31, 212, 1030, 1066, 1247, 1293
HspAI GCGC 4 cut(s) 47, 323, 425, 630
Kzo9I GATC 3 cut(s) 55, 60, 280
LmnI GCTCC 2 cut(s) 330, 766
Lsp1109I GCAGC 4 cut(s) 35, 414, 494, 916
LweI GCATC 3 cut(s) 10, 642, 908
MaeI CTAG 3 cut(s) 140, 663, 1193
MaeII ACGT 6 cut(s) 126, 228, 330, 775, 1239, 1261
MalI GATC 3 cut(s) 57, 62, 282
MboI GATC 3 cut(s) 55, 60, 280
MboII GAAGA 5 cut(s) 80, 689, 886, 1100, 1412
MflI RGATCY 2 cut(s) 55, 280
MhlI GDGCHC 4 cut(s) 249, 715, 747, 763
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 3 cut(s) 508, 578, 1104
MluI ACGCGT 1 cut(s) 612
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 4 cut(s) 197, 388, 398, 667
MmeI TCCRAC 2 cut(s) 435, 788
Mox20I TGGCCA 1 cut(s) 554
MscI TGGCCA 1 cut(s) 554
MseI TTAA 6 cut(s) 51, 354, 1071, 1301, 1371, 1420
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 4 cut(s) 284, 451, 597, 836
MspR9I CCNGG 3 cut(s) 149, 285, 945
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 2 cut(s) 149, 945
MvnI CGCG 1 cut(s) 614
MwoI GCNNNNNNNGC 7 cut(s) 29, 205, 620, 629, 719, 917, 926
NciI CCSGG 1 cut(s) 285
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 1224
NdeII GATC 3 cut(s) 55, 60, 280
NlaIII CATG 6 cut(s) 31, 212, 1030, 1066, 1247, 1293
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 3 cut(s) 143, 1183, 1227
NotI GCGGCCGC 1 cut(s) 336
NspI RCATGY 1 cut(s) 1247
PaeR7I CTCGAG 1 cut(s) 811
PagI TCATGA 1 cut(s) 1062
PciI ACATGT 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 1 cut(s) 625
PfeI GAWTC 4 cut(s) 692, 1000, 1339, 1348
PinAI ACCGGT 1 cut(s) 450
PleI GAGTC 4 cut(s) 197, 388, 397, 666
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
PpsI GAGTC 4 cut(s) 197, 388, 397, 666
Ppu21I YACGTR 4 cut(s) 127, 229, 331, 1262
PscI ACATGT 1 cut(s) 1243
Psp6I CCWGG 2 cut(s) 147, 943
PspCI CACGTG 1 cut(s) 229
PspEI GGTNACC 2 cut(s) 143, 1267
PspFI CCCAGC 3 cut(s) 485, 707, 839
PspGI CCWGG 2 cut(s) 147, 943
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 373, 409
PsuI RGATCY 2 cut(s) 55, 280
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 1242
RsaNI GTAC 1 cut(s) 1241
RseI CAYNNNNRTG 1 cut(s) 650
Rsr2I CGGWCCG 1 cut(s) 409
RsrII CGGWCCG 1 cut(s) 409
SalI GTCGAC 1 cut(s) 391
SaqAI TTAA 6 cut(s) 51, 354, 1071, 1301, 1371, 1420
Sau3AI GATC 3 cut(s) 55, 60, 280
Sau96I GGNCC 2 cut(s) 373, 409
SchI GAGTC 4 cut(s) 197, 388, 398, 667
ScrFI CCNGG 3 cut(s) 149, 285, 945
SduI GDGCHC 4 cut(s) 249, 715, 747, 763
SfaNI GCATC 3 cut(s) 10, 642, 908
Sfr274I CTCGAG 1 cut(s) 811
SinI GGWCC 2 cut(s) 373, 409
SlaI CTCGAG 1 cut(s) 811
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
SnaBI TACGTA 1 cut(s) 1262
SpeI ACTAGT 1 cut(s) 1192
Sse9I AATT 3 cut(s) 508, 578, 1104
SspMI CTAG 3 cut(s) 140, 663, 1193
StyD4I CCNGG 3 cut(s) 147, 283, 943
StyI CCWWGG 2 cut(s) 27, 342
TaaI ACNGT 5 cut(s) 527, 535, 807, 916, 1012
TaiI ACGT 6 cut(s) 129, 231, 333, 778, 1242, 1264
TaqI TCGA 6 cut(s) 84, 392, 477, 700, 793, 812
TaqII GACCGA 1 cut(s) 426
TasI AATT 3 cut(s) 508, 578, 1104
TauI GCSGC 7 cut(s) 47, 121, 177, 236, 239, 338, 341
TfiI GAWTC 4 cut(s) 692, 1000, 1339, 1348
Tru1I TTAA 6 cut(s) 51, 354, 1071, 1301, 1371, 1420
Tru9I TTAA 6 cut(s) 51, 354, 1071, 1301, 1371, 1420
TscAI CASTG 3 cut(s) 46, 538, 810
TseFI GTSAC 3 cut(s) 143, 1183, 1227
TseI GCWGC 4 cut(s) 23, 427, 482, 929
Tsp45I GTSAC 3 cut(s) 143, 1183, 1227
TspDTI ATGAA 7 cut(s) 845, 873, 993, 1051, 1198, 1352, 1407
TspGWI ACGGA 4 cut(s) 321, 422, 1153, 1208
TspRI CASTG 3 cut(s) 46, 538, 810
VneI GTGCAC 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 373, 409
XapI RAATTY 1 cut(s) 578
XceI RCATGY 1 cut(s) 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XhoI CTCGAG 1 cut(s) 811
XmiI GTMKAC 2 cut(s) 392, 1386
XspI CTAG 3 cut(s) 140, 663, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.