Rroxscaffold_7G00157580

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002261
Physical Location & Seq
Forward (+)
1374768 .. 1377395
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157580.1

Sequence Viewer

Length: 1251 bp
ATGGAAGAAGAGCTTCACCAAGCAGCCCTGAAGGGCTCCGTACCTGCTCTCCTCGACCTCCTCAGACAACACCCTCATATCCTCAACGGGACCCCATCTCTCTCCGACACCCCCTTACACGTCGCCGCCATTCTCGGCCACTCCGCCTTCGCCGCCGAGTTACTCACCCGGAGCCCCGAACTCGCCTCCCGGCTCAACCTCCAAGGCTCCTCGCCGCTCCACCTGGCAGCAGCAAAAGGTTGCGTGGAGATAGTCAAAAGTCTGGTGATGGTCAACGCTGATCTGGGCTTGGTCTGGGATCGGGATGGGTTGACTCCTCTGCATCTGGGGGTGATCAAGGGCCGGGTTGGGGTGGTGGCCGAGTTGGCCCGGGTTAGACCGGAGGCGGCTCGGGTGTTGACTCAGGGAGGGGAGAGCGGGTTTCACTTGTGTGTGAAGCATCATAGGTTTGAGGTGTTGAAGGTTTTGGTGGGGTGTGTTGGGAAAGATGATGACTTTGTGAATTGGAGGGATGGGGATGGCAACACTGTTTTGCATGTGGCTGTGGCCAACAAGCAATTAGAGGTGATAAATTACTTGCTGGATAATACAAAGATCGAAGTAAATGCCCAAAATTCAAATGGCTTCACCGCATTGGATGTCTTATCACATAGTACTAGAGATTTACGAGATTTGGAAATCAAAGAATCCCTTCAATTTGCCGGAGGGTCAAAAATTATTAAGAAGGCACCGTCTGTTATGTTTGATCAAGACACAGTGCAAGTTCCTACCACAATGCAGCCACTGATGTCAAAGAGGAGGAGTTTAAGGCAACTAGTATTTAAGCCGAAGCAGAAAGAAGTTGATTGGTTGGGTAGGAAACGAAGTTCGTTAATGGTTGTGGCTTCACTAATTGCTACAGTGGCATTTCAGTCTGCAATAAACCCGCCAGGTGGTGTTTGGCAGTCGGACTACCTGGAAGATAGCAATGGCAACCCTGTGGATAAACCACACCATGCTGGGCAGTCAGTGATGGCCGATACAGAACCAAGTCAGTATGGACTGTTCATGATTTCCAATACACTTGCTTTCCTTTCATCACTGAGCATAATTCTATTACTTGTGAGTGGATTGCCTATGAAGCGGCGCAGATGGATGTGGATTCAGATGGTGATCATGTGGGTTGCAATCACAGCGCTAACAGCTACGTATTTTCTTGGACTGATATTTATGACACCACATCAGCAGAAGGGGGTTATCTGTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

416

Amino Acids

45.68

Weight (kDa)

8.59

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 95 2.2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 67 - 123 2.8e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 141 - 213 2.9e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 167 - 214 6.3e-07 Ankyrin repeats (many copies)
PGG PF13962 282 - 403 6.2e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 727
AccBSI CCGCTC 2 cut(s) 217, 417
AciI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
AclWI GGATC 1 cut(s) 306
AcoI YGGCCR 4 cut(s) 136, 357, 546, 1014
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 2 cut(s) 42, 655
AfeI AGCGCT 1 cut(s) 1176
AfiI CCNNNNNNNGG 2 cut(s) 349, 932
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 3 cut(s) 460, 618, 695
AhlI ACTAGT 1 cut(s) 814
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 3 cut(s) 222, 928, 954
AleI CACNNNNGTG 1 cut(s) 429
AloI GAACNNNNNNTCC 2 cut(s) 850, 882
AluBI AGCT 2 cut(s) 13, 1184
AluI AGCT 2 cut(s) 13, 1184
AlwI GGATC 1 cut(s) 306
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 2 cut(s) 369, 390
Aor51HI AGCGCT 1 cut(s) 1176
AoxI GGCC 6 cut(s) 136, 340, 357, 366, 546, 1014
ApeKI GCWGC 4 cut(s) 23, 227, 230, 778
ApoI RAATTY 1 cut(s) 613
Asp700I GAANNNNTTC 2 cut(s) 12, 690
AspLEI GCGC 2 cut(s) 1128, 1177
AspS9I GGNCC 3 cut(s) 90, 340, 367
AsuC2I CCSGG 5 cut(s) 169, 190, 344, 370, 371
AsuHPI GGTGA 7 cut(s) 8, 157, 277, 343, 577, 619, 1162
AvaI CYCGRG 2 cut(s) 369, 390
AvaII GGWCC 1 cut(s) 90
BalI TGGCCA 1 cut(s) 548
BanI GGYRCC 1 cut(s) 727
BanII GRGCYC 2 cut(s) 38, 176
BbvI GCAGC 4 cut(s) 35, 239, 242, 790
BccI CCATC 8 cut(s) 103, 262, 299, 506, 512, 1006, 1125, 1141
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 3 cut(s) 224, 930, 956
BclI TGATCA 3 cut(s) 333, 745, 1152
BcnI CCSGG 5 cut(s) 169, 190, 344, 370, 371
BcuI ACTAGT 1 cut(s) 814
BfaI CTAG 2 cut(s) 657, 815
BfmI CTRYAG 1 cut(s) 897
BfoI RGCGCY 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 52
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 9 cut(s) 24, 126, 153, 215, 228, 231, 387, 779, 1124
BlsI GCNGC 9 cut(s) 25, 127, 154, 216, 229, 232, 388, 780, 1125
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 8 cut(s) 169, 190, 224, 344, 370, 371, 930, 956
Bme18I GGWCC 1 cut(s) 90
BmeT110I CYCGRG 2 cut(s) 369, 390
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 90, 340, 367
BmiI GGNNCC 6 cut(s) 37, 91, 92, 173, 208, 729
BmrFI CCNGG 8 cut(s) 169, 190, 224, 344, 370, 371, 930, 956
BmsI GCATC 2 cut(s) 331, 448
BpuMI CCSGG 5 cut(s) 169, 190, 344, 370, 371
BsaAI YACGTR 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 1151
BsaJI CCNNGG 2 cut(s) 202, 369
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 404, 434
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 932
Bse3DI GCAATG 1 cut(s) 973
Bse8I GATNNNNATC 1 cut(s) 1151
BseBI CCWGG 3 cut(s) 224, 930, 956
BseDI CCNNGG 2 cut(s) 202, 369
BseGI GGATG 5 cut(s) 310, 517, 523, 643, 1140
BseJI GATNNNNATC 1 cut(s) 1151
BseLI CCNNNNNNNGG 2 cut(s) 349, 932
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 3 cut(s) 76, 416, 1073
BseRI GAGGAG 6 cut(s) 41, 50, 199, 306, 811, 814
BseXI GCAGC 4 cut(s) 35, 239, 242, 790
BseYI CCCAGC 1 cut(s) 998
BshFI GGCC 6 cut(s) 138, 342, 359, 368, 548, 1016
BshNI GGYRCC 1 cut(s) 727
BsiHKCI CYCGRG 2 cut(s) 369, 390
BsiSI CCGG 6 cut(s) 169, 190, 343, 370, 380, 702
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 2 cut(s) 349, 932
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 6 cut(s) 138, 342, 359, 368, 548, 1016
BsoBI CYCGRG 2 cut(s) 369, 390
Bsp1286I GDGCHC 2 cut(s) 38, 176
Bsp143I GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
BspACI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
BspANI GGCC 6 cut(s) 138, 342, 359, 368, 548, 1016
BspCNI CTCAG 3 cut(s) 75, 415, 1074
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 6 cut(s) 37, 91, 92, 173, 208, 729
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 1 cut(s) 306
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 1 cut(s) 727
BsrBI CCGCTC 2 cut(s) 217, 417
BsrDI GCAATG 1 cut(s) 973
BssECI CCNNGG 2 cut(s) 202, 369
BssMI GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 3 cut(s) 224, 930, 956
Bst4CI ACNGT 5 cut(s) 529, 732, 757, 901, 1044
Bst6I CTCTTC 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 1188
BstDEI CTNAG 3 cut(s) 62, 402, 1082
BstF5I GGATG 5 cut(s) 310, 517, 523, 643, 1140
BstH2I RGCGCY 1 cut(s) 1178
BstHHI GCGC 2 cut(s) 1128, 1177
BstKTI GATC 6 cut(s) 283, 301, 336, 597, 748, 1155
BstMBI GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
BstMWI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
BstNI CCWGG 3 cut(s) 224, 930, 956
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 8 cut(s) 167, 188, 222, 342, 368, 369, 928, 954
BstSFI CTRYAG 1 cut(s) 897
BstSNI TACGTA 1 cut(s) 1188
BstV1I GCAGC 4 cut(s) 35, 239, 242, 790
BsuRI GGCC 6 cut(s) 138, 342, 359, 368, 548, 1016
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 5 cut(s) 310, 517, 523, 643, 1140
BtsIMutI CAGTG 6 cut(s) 525, 762, 782, 906, 1014, 1079
BveI ACCTGC 1 cut(s) 52
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 2 cut(s) 1128, 1177
Cfr13I GGNCC 3 cut(s) 90, 340, 367
Cfr9I CCCGGG 1 cut(s) 369
Csp6I GTAC 2 cut(s) 41, 654
CviAII CATG 5 cut(s) 536, 995, 1048, 1156, 1246
CviQI GTAC 2 cut(s) 41, 654
DdeI CTNAG 3 cut(s) 62, 402, 1082
DpnI GATC 6 cut(s) 282, 300, 335, 596, 747, 1154
DpnII GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
EaeI YGGCCR 4 cut(s) 136, 357, 546, 1014
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
EciI GGCGGA 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 1188
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 38, 176
Eco47I GGWCC 1 cut(s) 90
Eco47III AGCGCT 1 cut(s) 1176
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 369, 390
EcoO109I RGGNCCY 1 cut(s) 90
EcoRII CCWGG 3 cut(s) 222, 928, 954
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 2 cut(s) 38, 176
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 5 cut(s) 539, 998, 1051, 1159, 1249
FalI AAGNNNNNCTT 3 cut(s) 29, 675, 707
FaqI GGGAC 1 cut(s) 103
FatI CATG 5 cut(s) 535, 994, 1047, 1155, 1245
FauI CCCGC 2 cut(s) 410, 933
FbaI TGATCA 3 cut(s) 333, 745, 1152
Fnu4HI GCNGC 9 cut(s) 24, 126, 153, 215, 228, 231, 387, 779, 1124
FokI GGATG 5 cut(s) 317, 524, 530, 650, 1147
FriOI GRGCYC 2 cut(s) 38, 176
Fsp4HI GCNGC 9 cut(s) 24, 126, 153, 215, 228, 231, 387, 779, 1124
FspBI CTAG 2 cut(s) 657, 815
GlaI GCGC 2 cut(s) 1127, 1176
GluI GCNGC 9 cut(s) 24, 126, 153, 215, 228, 231, 387, 779, 1124
GsaI CCCAGC 1 cut(s) 1002
HaeII RGCGCY 1 cut(s) 1178
HaeIII GGCC 6 cut(s) 138, 342, 359, 368, 548, 1016
HapII CCGG 6 cut(s) 169, 190, 343, 370, 380, 702
HhaI GCGC 2 cut(s) 1128, 1177
Hin1II CATG 5 cut(s) 539, 998, 1051, 1159, 1249
Hin6I GCGC 2 cut(s) 1126, 1175
HinP1I GCGC 2 cut(s) 1126, 1175
HincII GTYRAC 3 cut(s) 274, 312, 399
HindII GTYRAC 3 cut(s) 274, 312, 399
HinfI GANTC 4 cut(s) 313, 400, 686, 1141
HpaII CCGG 6 cut(s) 169, 190, 343, 370, 380, 702
HphI GGTGA 7 cut(s) 8, 157, 277, 343, 577, 619, 1162
Hpy166II GTNNAC 3 cut(s) 274, 312, 399
Hpy188I TCNGA 4 cut(s) 65, 106, 949, 1146
Hpy188III TCNNGA 3 cut(s) 302, 749, 1048
Hpy8I GTNNAC 3 cut(s) 274, 312, 399
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 6 cut(s) 25, 157, 454, 701, 718, 1222
HpyCH4III ACNGT 5 cut(s) 529, 732, 757, 901, 1044
HpyCH4IV ACGT 2 cut(s) 120, 1187
HpyCH4V TGCA 6 cut(s) 322, 535, 760, 778, 917, 1166
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
HpyF3I CTNAG 3 cut(s) 62, 402, 1082
HpySE526I ACGT 2 cut(s) 120, 1187
Hsp92II CATG 5 cut(s) 539, 998, 1051, 1159, 1249
HspAI GCGC 2 cut(s) 1126, 1175
KflI GGGWCCC 1 cut(s) 90
Ksp22I TGATCA 3 cut(s) 333, 745, 1152
Kzo9I GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 4 cut(s) 41, 171, 212, 222
Lsp1109I GCAGC 4 cut(s) 35, 239, 242, 790
LweI GCATC 2 cut(s) 331, 448
MaeI CTAG 2 cut(s) 657, 815
MaeII ACGT 2 cut(s) 120, 1187
MaeIII GTNAC 1 cut(s) 159
MalI GATC 6 cut(s) 282, 300, 335, 596, 747, 1154
MbiI CCGCTC 2 cut(s) 217, 417
MboI GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
MboII GAAGA 3 cut(s) 17, 20, 971
MhlI GDGCHC 2 cut(s) 38, 176
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 2 cut(s) 307, 394
MmeI TCCRAC 2 cut(s) 129, 927
Mox20I TGGCCA 1 cut(s) 548
MroXI GAANNNNTTC 2 cut(s) 12, 690
MscI TGGCCA 1 cut(s) 548
MseI TTAA 4 cut(s) 720, 806, 822, 872
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 548
MspI CCGG 6 cut(s) 169, 190, 343, 370, 380, 702
MspR9I CCNGG 8 cut(s) 169, 190, 224, 344, 370, 371, 930, 956
MvaI CCWGG 3 cut(s) 224, 930, 956
MwoI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
NciI CCSGG 5 cut(s) 169, 190, 344, 370, 371
NdeII GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
NlaIII CATG 5 cut(s) 539, 998, 1051, 1159, 1249
NlaIV GGNNCC 6 cut(s) 37, 91, 92, 173, 208, 729
NmeAIII GCCGAG 3 cut(s) 114, 181, 385
NspI RCATGY 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 429
PagI TCATGA 1 cut(s) 1047
PciSI GCTCTTC 1 cut(s) 3
PdmI GAANNNNTTC 2 cut(s) 12, 690
PfeI GAWTC 2 cut(s) 686, 1141
PkrI GCNGC 9 cut(s) 25, 127, 154, 216, 229, 232, 388, 780, 1125
PleI GAGTC 2 cut(s) 307, 394
PpsI GAGTC 2 cut(s) 307, 394
Ppu21I YACGTR 1 cut(s) 1188
PpuMI RGGWCCY 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 90
Psp6I CCWGG 3 cut(s) 222, 928, 954
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 3 cut(s) 222, 928, 954
PspN4I GGNNCC 6 cut(s) 37, 91, 92, 173, 208, 729
PspPI GGNCC 3 cut(s) 90, 340, 367
PspPPI RGGWCCY 1 cut(s) 90
PsrI GAACNNNNNNTAC 2 cut(s) 1028, 1060
PstNI CAGNNNCTG 1 cut(s) 784
RsaI GTAC 2 cut(s) 42, 655
RsaNI GTAC 2 cut(s) 41, 654
RseI CAYNNNNRTG 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 3
SaqAI TTAA 4 cut(s) 720, 806, 822, 872
SatI GCNGC 9 cut(s) 24, 126, 153, 215, 228, 231, 387, 779, 1124
Sau3AI GATC 6 cut(s) 280, 298, 333, 594, 745, 1152
Sau96I GGNCC 3 cut(s) 90, 340, 367
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 307, 394
ScrFI CCNGG 8 cut(s) 169, 190, 224, 344, 370, 371, 930, 956
SduI GDGCHC 2 cut(s) 38, 176
SfaNI GCATC 2 cut(s) 331, 448
SfcI CTRYAG 1 cut(s) 897
SfiI GGCCNNNNNGGCC 1 cut(s) 365
SinI GGWCC 1 cut(s) 90
SmaI CCCGGG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 429
SnaBI TACGTA 1 cut(s) 1188
SpeI ACTAGT 1 cut(s) 814
Sse9I AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
SsiI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
SspMI CTAG 2 cut(s) 657, 815
StyD4I CCNGG 8 cut(s) 167, 188, 222, 342, 368, 369, 928, 954
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 5 cut(s) 529, 732, 757, 901, 1044
TaiI ACGT 2 cut(s) 123, 1190
TaqI TCGA 2 cut(s) 54, 597
TasI AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
TatI WGTACW 1 cut(s) 653
TauI GCSGC 5 cut(s) 128, 155, 217, 389, 1126
TfiI GAWTC 2 cut(s) 686, 1141
Tru1I TTAA 4 cut(s) 720, 806, 822, 872
Tru9I TTAA 4 cut(s) 720, 806, 822, 872
TscAI CASTG 6 cut(s) 532, 762, 789, 906, 1014, 1086
TseI GCWGC 4 cut(s) 23, 227, 230, 778
TspDTI ATGAA 3 cut(s) 1036, 1065, 1133
TspGWI ACGGA 1 cut(s) 28
TspMI CCCGGG 1 cut(s) 369
TspRI CASTG 6 cut(s) 532, 762, 789, 906, 1014, 1086
VpaK11BI GGWCC 1 cut(s) 90
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 936
XmaI CCCGGG 1 cut(s) 369
XmnI GAANNNNTTC 2 cut(s) 12, 690
XspI CTAG 2 cut(s) 657, 815
ZrmI AGTACT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.