Rh6BG514400

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002261
Physical Location & Seq
Reverse (-)
70896259 .. 70899432
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG514400.1

Sequence Viewer

Length: 1410 bp
ATGGAAGAAGAGCTTCACCAAGCAGCCCTGAAGGGCTCCGTACCTGCTCTCCTCGACCTCCTCAGACAACACCCTCATATCCTCAACGGCACCCCATCTCTCTCCGACACCCCCTTACACGTCGCCGCCATTCTCGGCCACTCCGCCTTCGCCGCCGAGTTACTCACCCGGAGCCCCGAACTCGCCTCCCGGCTCAACCTCCAAGGCTCCTCGCCGCTCCACCTGGCAGCAGCAAAAGGGTGCTTGGAGATAGTCAAAAGTCTGGTGCTGGTCAACGCTGATCTGGGCTTGGTCTGGGATCGGGATGGGTTGACTCCTCTGCATCTGGGGGTGATCAAGGGCCGGGTTGGGGTGGTGGCTGAGTTGGCCCGAGTTAGACCGGAGGCGGCTCGGGTGTTGACTCAGGGAGGGGAGAGCGGGTTTCACTTGTGTGTGAAGCATCATAGGTTTGAGGTGTTGAAGGTTTTGGTGGGGTGTGTTGGGAAAGATGATGACTTTGTGAATTGGAGGGATGGGGATGGCAACACTGTTTTGCATGTGGCTGTGGCCAAGAAACAATTAGAGGTGATAAATTACTTGCTGGATAATACAAAGATCGAAGTAAATGCCCAAAATTCAAATGGCTTCACCGCATTGGATGTCTTATCGCATAGTACTAGAGATTTACGAGACTTGGAAATCAAAGAAACCCTTCAATTTGCCGGAGCTTCAAAAATTATTAAGAAGGCACCTTCTGTTATGTTTGATCAAGATACAGTGCAAGTTCCTACCACAATGCAGCCACTGATGTCAAAGAGGAGGAGTTTAAGGCAACTAGTATTTAAGCCGAAGCAGAAAGAAGTTGATTGGTTGGCTAGGAAACGAAGTTCGTTAATGGTTGTGGCTTCACTAATTGCTACAGTGGCATTTCAGTCTGCAATAAACCCGCCAGGTGGAGTTTGGCAGTCGGACTATCTGGAAGATAGCAATGGCAACCCTGTGGATAAACCACACCATGCTGGGCAGTCAGTGATGGCCGATACAGAACCAAGTCAGTATGGACTGTTCATGATTTCCAATACACTTGCTTTCCTTTCATCACTGAGCATAATTCTATTACTTGTGAGTGGATTGCCTATGAAGCGGCGCAGATGGATGTGGATTCAGATGGTGATCATGTGGGTTGCAATCACAGCGCTAACAGCTACGTATTTTCTTGGAATGATATTTATGACACCACAACAGCAGAAGGGGGGTTATCTGTATCATGTGACCCAAGTTTCTGTGCTTGTATGGATGGCACTGATGGGGATCGTGTTTATCGGTAATGTTGTTCGGGCAGCAAGATGGCTACTTAGAAAGTATGGATGCATGAAGCAGAAACCTATAGATGATTCGCTTTATGAAGATTATGACATTGATGACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

469

Amino Acids

51.95

Weight (kDa)

8.52

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 95 4.2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 68 - 123 8.2e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 73 - 119 5.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 131 - 205 6.8e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 141 - 214 7e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 167 - 214 9.8e-07 Ankyrin repeats (many copies)
PGG PF13962 282 - 403 4.4e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AccB1I GGYRCC 2 cut(s) 89, 727
AccBSI CCGCTC 2 cut(s) 217, 417
AciI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
AclWI GGATC 2 cut(s) 306, 1298
AcoI YGGCCR 3 cut(s) 136, 546, 1014
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 2 cut(s) 42, 655
AfeI AGCGCT 1 cut(s) 1176
AfiI CCNNNNNNNGG 2 cut(s) 349, 932
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 4 cut(s) 460, 618, 695, 711
AhlI ACTAGT 1 cut(s) 814
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 2 cut(s) 222, 928
AleI CACNNNNGTG 1 cut(s) 429
AloI GAACNNNNNNTCC 2 cut(s) 850, 882
AluBI AGCT 3 cut(s) 13, 707, 1184
AluI AGCT 3 cut(s) 13, 707, 1184
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 2 cut(s) 306, 1298
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 2 cut(s) 369, 390
Aor51HI AGCGCT 1 cut(s) 1176
AoxI GGCC 5 cut(s) 136, 340, 366, 546, 1014
ApeKI GCWGC 5 cut(s) 23, 227, 230, 778, 1319
ApoI RAATTY 1 cut(s) 613
Asp700I GAANNNNTTC 2 cut(s) 12, 690
AspLEI GCGC 2 cut(s) 1128, 1177
AspS9I GGNCC 2 cut(s) 340, 367
AsuC2I CCSGG 3 cut(s) 169, 190, 344
AsuHPI GGTGA 6 cut(s) 8, 157, 343, 577, 619, 1162
AvaI CYCGRG 2 cut(s) 369, 390
BalI TGGCCA 1 cut(s) 548
BanI GGYRCC 2 cut(s) 89, 727
BanII GRGCYC 2 cut(s) 38, 176
BbvI GCAGC 5 cut(s) 35, 239, 242, 790, 1331
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 2 cut(s) 224, 930
BclI TGATCA 3 cut(s) 333, 745, 1152
BcnI CCSGG 3 cut(s) 169, 190, 344
BcoDI GTCTC 1 cut(s) 663
BcuI ACTAGT 1 cut(s) 814
BfaI CTAG 3 cut(s) 657, 815, 855
BfmI CTRYAG 2 cut(s) 897, 1365
BfoI RGCGCY 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 52
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 5 cut(s) 169, 190, 224, 344, 930
BmeT110I CYCGRG 2 cut(s) 369, 390
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 340, 367
BmiI GGNNCC 5 cut(s) 37, 91, 173, 208, 729
BmrFI CCNGG 5 cut(s) 169, 190, 224, 344, 930
BmsI GCATC 3 cut(s) 331, 448, 1337
BpuMI CCSGG 3 cut(s) 169, 190, 344
BsaAI YACGTR 1 cut(s) 1188
BsaBI GATNNNNATC 2 cut(s) 1151, 1289
BsaJI CCNNGG 1 cut(s) 202
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 404, 434
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 932
Bse3DI GCAATG 1 cut(s) 973
Bse8I GATNNNNATC 2 cut(s) 1151, 1289
BseBI CCWGG 2 cut(s) 224, 930
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 7 cut(s) 310, 517, 523, 643, 1140, 1281, 1352
BseJI GATNNNNATC 2 cut(s) 1151, 1289
BseLI CCNNNNNNNGG 2 cut(s) 349, 932
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 4 cut(s) 76, 351, 416, 1073
BseRI GAGGAG 6 cut(s) 41, 50, 199, 306, 811, 814
BseXI GCAGC 5 cut(s) 35, 239, 242, 790, 1331
BseYI CCCAGC 1 cut(s) 998
BshFI GGCC 5 cut(s) 138, 342, 368, 548, 1016
BshNI GGYRCC 2 cut(s) 89, 727
BsiHKCI CYCGRG 2 cut(s) 369, 390
BsiSI CCGG 5 cut(s) 169, 190, 343, 380, 702
BslI CCNNNNNNNGG 2 cut(s) 349, 932
BsmAI GTCTC 1 cut(s) 663
BsnI GGCC 5 cut(s) 138, 342, 368, 548, 1016
BsoBI CYCGRG 2 cut(s) 369, 390
Bsp1286I GDGCHC 2 cut(s) 38, 176
Bsp143I GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
BspACI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
BspANI GGCC 5 cut(s) 138, 342, 368, 548, 1016
BspCNI CTCAG 4 cut(s) 75, 352, 415, 1074
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 5 cut(s) 37, 91, 173, 208, 729
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 2 cut(s) 306, 1298
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 2 cut(s) 89, 727
BsrBI CCGCTC 2 cut(s) 217, 417
BsrDI GCAATG 1 cut(s) 973
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 224, 930
Bst4CI ACNGT 4 cut(s) 529, 757, 901, 1044
Bst6I CTCTTC 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 1188
BstDEI CTNAG 5 cut(s) 62, 360, 402, 1082, 1334
BstF5I GGATG 7 cut(s) 310, 517, 523, 643, 1140, 1281, 1352
BstH2I RGCGCY 1 cut(s) 1178
BstHHI GCGC 2 cut(s) 1128, 1177
BstKTI GATC 7 cut(s) 283, 301, 336, 597, 748, 1155, 1293
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
BstMWI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
BstNI CCWGG 2 cut(s) 224, 930
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 5 cut(s) 167, 188, 222, 342, 928
BstSFI CTRYAG 2 cut(s) 897, 1365
BstSNI TACGTA 1 cut(s) 1188
BstV1I GCAGC 5 cut(s) 35, 239, 242, 790, 1331
BsuRI GGCC 5 cut(s) 138, 342, 368, 548, 1016
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 7 cut(s) 310, 517, 523, 643, 1140, 1281, 1352
BtsIMutI CAGTG 7 cut(s) 525, 762, 782, 906, 1014, 1079, 1280
BveI ACCTGC 1 cut(s) 52
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 2 cut(s) 1128, 1177
Cfr13I GGNCC 2 cut(s) 340, 367
Csp6I GTAC 2 cut(s) 41, 654
CviAII CATG 6 cut(s) 536, 995, 1048, 1156, 1247, 1351
CviQI GTAC 2 cut(s) 41, 654
DdeI CTNAG 5 cut(s) 62, 360, 402, 1082, 1334
DpnI GATC 7 cut(s) 282, 300, 335, 596, 747, 1154, 1292
DpnII GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
EaeI YGGCCR 3 cut(s) 136, 546, 1014
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
EciI GGCGGA 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 1188
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 38, 176
Eco47III AGCGCT 1 cut(s) 1176
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 369, 390
EcoRII CCWGG 2 cut(s) 222, 928
EcoT14I CCWWGG 1 cut(s) 202
EcoT22I ATGCAT 1 cut(s) 1352
EcoT38I GRGCYC 2 cut(s) 38, 176
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 6 cut(s) 539, 998, 1051, 1159, 1250, 1354
FalI AAGNNNNNCTT 3 cut(s) 29, 675, 707
FatI CATG 6 cut(s) 535, 994, 1047, 1155, 1246, 1350
FauI CCCGC 2 cut(s) 410, 933
FbaI TGATCA 3 cut(s) 333, 745, 1152
FokI GGATG 7 cut(s) 317, 524, 530, 650, 1147, 1288, 1359
FriOI GRGCYC 2 cut(s) 38, 176
FspBI CTAG 3 cut(s) 657, 815, 855
GlaI GCGC 2 cut(s) 1127, 1176
GsaI CCCAGC 1 cut(s) 1002
HaeII RGCGCY 1 cut(s) 1178
HaeIII GGCC 5 cut(s) 138, 342, 368, 548, 1016
HapII CCGG 5 cut(s) 169, 190, 343, 380, 702
HhaI GCGC 2 cut(s) 1128, 1177
Hin1II CATG 6 cut(s) 539, 998, 1051, 1159, 1250, 1354
Hin6I GCGC 2 cut(s) 1126, 1175
HinP1I GCGC 2 cut(s) 1126, 1175
HincII GTYRAC 3 cut(s) 274, 312, 399
HindII GTYRAC 3 cut(s) 274, 312, 399
HinfI GANTC 4 cut(s) 313, 400, 1141, 1373
HpaII CCGG 5 cut(s) 169, 190, 343, 380, 702
HphI GGTGA 6 cut(s) 8, 157, 343, 577, 619, 1162
Hpy166II GTNNAC 3 cut(s) 274, 312, 399
Hpy188I TCNGA 4 cut(s) 65, 106, 949, 1146
Hpy188III TCNNGA 4 cut(s) 302, 749, 956, 1048
Hpy8I GTNNAC 3 cut(s) 274, 312, 399
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 7 cut(s) 25, 157, 454, 701, 718, 741, 1222
HpyCH4III ACNGT 4 cut(s) 529, 757, 901, 1044
HpyCH4IV ACGT 2 cut(s) 120, 1187
HpyCH4V TGCA 7 cut(s) 322, 535, 760, 778, 917, 1166, 1350
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
HpyF3I CTNAG 5 cut(s) 62, 360, 402, 1082, 1334
HpySE526I ACGT 2 cut(s) 120, 1187
Hsp92II CATG 6 cut(s) 539, 998, 1051, 1159, 1250, 1354
HspAI GCGC 2 cut(s) 1126, 1175
Ksp22I TGATCA 3 cut(s) 333, 745, 1152
Kzo9I GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 5 cut(s) 41, 171, 212, 222, 704
Lsp1109I GCAGC 5 cut(s) 35, 239, 242, 790, 1331
LweI GCATC 3 cut(s) 331, 448, 1337
MaeI CTAG 3 cut(s) 657, 815, 855
MaeII ACGT 2 cut(s) 120, 1187
MaeIII GTNAC 2 cut(s) 159, 1249
MalI GATC 7 cut(s) 282, 300, 335, 596, 747, 1154, 1292
MbiI CCGCTC 2 cut(s) 217, 417
MboI GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
MboII GAAGA 4 cut(s) 17, 20, 971, 1397
MhlI GDGCHC 2 cut(s) 38, 176
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 2 cut(s) 307, 394
MmeI TCCRAC 2 cut(s) 129, 927
Mox20I TGGCCA 1 cut(s) 548
Mph1103I ATGCAT 1 cut(s) 1352
MroXI GAANNNNTTC 2 cut(s) 12, 690
MscI TGGCCA 1 cut(s) 548
MseI TTAA 4 cut(s) 720, 806, 822, 872
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 548
MspI CCGG 5 cut(s) 169, 190, 343, 380, 702
MspR9I CCNGG 5 cut(s) 169, 190, 224, 344, 930
MvaI CCWGG 2 cut(s) 224, 930
MwoI GCNNNNNNNGC 6 cut(s) 152, 365, 902, 1120, 1172, 1181
NciI CCSGG 3 cut(s) 169, 190, 344
NdeII GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
NlaIII CATG 6 cut(s) 539, 998, 1051, 1159, 1250, 1354
NlaIV GGNNCC 5 cut(s) 37, 91, 173, 208, 729
NmeAIII GCCGAG 2 cut(s) 114, 181
NmuCI GTSAC 1 cut(s) 1249
NsiI ATGCAT 1 cut(s) 1352
NspI RCATGY 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 429
PagI TCATGA 1 cut(s) 1047
PciSI GCTCTTC 1 cut(s) 3
PdmI GAANNNNTTC 2 cut(s) 12, 690
PfeI GAWTC 2 cut(s) 1141, 1373
PleI GAGTC 2 cut(s) 307, 394
PpsI GAGTC 2 cut(s) 307, 394
Ppu21I YACGTR 1 cut(s) 1188
Psp6I CCWGG 2 cut(s) 222, 928
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 2 cut(s) 222, 928
PspN4I GGNNCC 5 cut(s) 37, 91, 173, 208, 729
PspPI GGNCC 2 cut(s) 340, 367
PsrI GAACNNNNNNTAC 2 cut(s) 1028, 1060
PstNI CAGNNNCTG 1 cut(s) 784
RsaI GTAC 2 cut(s) 42, 655
RsaNI GTAC 2 cut(s) 41, 654
RseI CAYNNNNRTG 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 3
SaqAI TTAA 4 cut(s) 720, 806, 822, 872
Sau3AI GATC 7 cut(s) 280, 298, 333, 594, 745, 1152, 1290
Sau96I GGNCC 2 cut(s) 340, 367
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 307, 394
ScrFI CCNGG 5 cut(s) 169, 190, 224, 344, 930
SduI GDGCHC 2 cut(s) 38, 176
SfaNI GCATC 3 cut(s) 331, 448, 1337
SfcI CTRYAG 2 cut(s) 897, 1365
SmiMI CAYNNNNRTG 1 cut(s) 429
SnaBI TACGTA 1 cut(s) 1188
SpeI ACTAGT 1 cut(s) 814
Sse9I AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
SsiI CCGC 9 cut(s) 126, 144, 153, 215, 386, 417, 630, 926, 1123
SspMI CTAG 3 cut(s) 657, 815, 855
StyD4I CCNGG 5 cut(s) 167, 188, 222, 342, 928
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 4 cut(s) 529, 757, 901, 1044
TaiI ACGT 2 cut(s) 123, 1190
TaqI TCGA 2 cut(s) 54, 597
TasI AATT 8 cut(s) 502, 557, 571, 613, 695, 714, 891, 1089
TatI WGTACW 1 cut(s) 653
TauI GCSGC 5 cut(s) 128, 155, 217, 389, 1126
TfiI GAWTC 2 cut(s) 1141, 1373
Tru1I TTAA 4 cut(s) 720, 806, 822, 872
Tru9I TTAA 4 cut(s) 720, 806, 822, 872
TscAI CASTG 7 cut(s) 532, 762, 789, 906, 1014, 1086, 1287
TseFI GTSAC 1 cut(s) 1249
TseI GCWGC 5 cut(s) 23, 227, 230, 778, 1319
Tsp45I GTSAC 1 cut(s) 1249
TspDTI ATGAA 5 cut(s) 1036, 1065, 1133, 1367, 1398
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 7 cut(s) 532, 762, 789, 906, 1014, 1086, 1287
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 936
XmnI GAANNNNTTC 2 cut(s) 12, 690
XspI CTAG 3 cut(s) 657, 815, 855
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 1 cut(s) 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.