Prupe.7G203800_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0002261
Physical Location & Seq
Forward (+)
18889848 .. 18892708
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G203800.1

Sequence Viewer

Length: 888 bp
ATGGAGAAAAAGCTTTTAGATGCGGCCATGGAGGGAAACATAACGATGCTTAAAGATTTAATCCAAGAAGACCCACTTGTCCTTGATAGAGCTTTAGCTTCCTGCCTCTCAGAGACCCCACTTCACATAGCTTCAATGTTGGGTCATCTGGGTTTCGTCAAGGAGCTACTGACTCATAAGCCCGAGTTGGCCTCCGAGCTGGACTCGAGGGGCTCGACGCCTCTCCACTTGGCGGCGGCCAAAGGGCACGCGGAGATCGTAAAGGAGCTGGCTTTGGTGGATCCTGCTGCATGCATGGTGAGGAACGAAGATGGGTGGACGGCGCTCCACGTGGCAGCCGTCAAGGGGCGTGTTAAGGTGCTGAGTGAGTTGGCCCGAGTCAGGACTGACTCCACTCTGGCGTTGACGGACCGAGGCGAGACTGTGCTGCACTTGTGCGTTGGGCATAATCGGTTGGAGGGGTTGAAGGCTTTGGCTGAGGCTTTTGGAAAAGATGATGAGTTTCTGAATTGGAAGGACGTCGATGGGAACACCATTTTGCATATTTCTGTGGCCAAGAAGCAAATTGAGATCATCAAGTTCTTGCTTACCAGTACTGGAGTGAATGTGAATGCGTTGAATGCCAACAGCTCTACGGCTTTGGACATTCTGATGCAGAGTCTTAGAGATTTGAGGGATATGGAGATTGAGGATTCTCTTCGAAGTGTAGGGGCTAAAAGTGCAAAGGATGTACAACCCATCATGCATGACTGGGTTCGTGTTCCAACAAAAGAACACCAGATATCAAGACGTCTAGGCTCAGCAGCACTATCTTCGAAAGAGGGTGATTCTATGAGGCTGCCCAACAGAAGCCTAGCGATTGGCTCGGGAGGAGGAGAAGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

296

Amino Acids

32.05

Weight (kDa)

6.13

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 2 cut(s) 522, 793
AccII CGCG 1 cut(s) 251
AciI CCGC 4 cut(s) 23, 233, 236, 251
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 3 cut(s) 24, 237, 552
AcvI CACGTG 1 cut(s) 331
AcyI GRCGYC 3 cut(s) 218, 519, 790
AfaI GTAC 2 cut(s) 595, 732
AfiI CCNNNNNNNGG 3 cut(s) 232, 345, 381
AgsI TTSAA 3 cut(s) 135, 466, 619
AloI GAACNNNNNNTCC 1 cut(s) 864
AluBI AGCT 8 cut(s) 13, 92, 98, 131, 166, 199, 268, 630
AluI AGCT 8 cut(s) 13, 92, 98, 131, 166, 199, 268, 630
Alw26I GTCTC 2 cut(s) 107, 413
AlwI GGATC 2 cut(s) 275, 288
Ama87I CYCGRG 4 cut(s) 182, 205, 375, 865
AoxI GGCC 5 cut(s) 24, 189, 237, 372, 552
ApeKI GCWGC 5 cut(s) 287, 335, 427, 803, 838
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 373, 409
AsuHPI GGTGA 2 cut(s) 310, 836
AsuII TTCGAA 2 cut(s) 700, 815
AvaI CYCGRG 4 cut(s) 182, 205, 375, 865
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 249
BalI TGGCCA 1 cut(s) 554
BamHI GGATCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 215
BbrPI CACGTG 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 75
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 274, 347, 414, 815, 825
BccI CCATC 3 cut(s) 305, 518, 746
BceAI ACGGC 3 cut(s) 323, 336, 651
BcgI CGANNNNNNTGC 2 cut(s) 795, 829
BcoDI GTCTC 2 cut(s) 107, 413
BfaI CTAG 2 cut(s) 794, 854
BfoI RGCGCY 1 cut(s) 326
BisI GCNGC 8 cut(s) 24, 234, 237, 288, 336, 428, 804, 839
BlpI GCTNAGC 1 cut(s) 799
BlsI GCNGC 8 cut(s) 25, 235, 238, 289, 337, 429, 805, 840
BmcAI AGTACT 1 cut(s) 595
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 4 cut(s) 182, 205, 375, 865
BmgT120I GGNCC 2 cut(s) 373, 409
BmiI GGNNCC 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 3 cut(s) 10, 36, 642
BmuI ACTGGG 1 cut(s) 760
BpiI GAAGAC 1 cut(s) 75
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 188, 220
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 799
Bpu14I TTCGAA 2 cut(s) 700, 815
BsaAI YACGTR 1 cut(s) 331
BsaHI GRCGYC 3 cut(s) 218, 519, 790
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 27, 412
BsaXI ACNNNNNCTCC 3 cut(s) 591, 621, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 345, 381
Bse1I ACTGG 3 cut(s) 591, 601, 755
BseDI CCNNGG 2 cut(s) 27, 412
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 3 cut(s) 232, 345, 381
BseMII CTCAG 4 cut(s) 123, 353, 468, 813
BseNI ACTGG 3 cut(s) 591, 601, 755
BseRI GAGGAG 2 cut(s) 885, 888
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 5 cut(s) 274, 347, 414, 815, 825
BsgI GTGCAG 1 cut(s) 413
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 5 cut(s) 26, 191, 239, 374, 554
BsiHKCI CYCGRG 4 cut(s) 182, 205, 375, 865
BslI CCNNNNNNNGG 3 cut(s) 232, 345, 381
BsmAI GTCTC 2 cut(s) 107, 413
BsmI GAATGC 2 cut(s) 616, 625
BsnI GGCC 5 cut(s) 26, 191, 239, 374, 554
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 4 cut(s) 182, 205, 375, 865
Bsp119I TTCGAA 2 cut(s) 700, 815
Bsp1286I GDGCHC 2 cut(s) 215, 249
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 3 cut(s) 255, 280, 570
Bsp1720I GCTNAGC 1 cut(s) 799
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 4 cut(s) 23, 233, 236, 251
BspANI GGCC 5 cut(s) 26, 191, 239, 374, 554
BspCNI CTCAG 4 cut(s) 122, 354, 469, 812
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 2 cut(s) 275, 288
BspT104I TTCGAA 2 cut(s) 700, 815
BspTNI GGTCTC 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 3 cut(s) 591, 601, 755
BssECI CCNNGG 2 cut(s) 27, 412
BssMI GATC 3 cut(s) 255, 280, 570
BssNI GRCGYC 3 cut(s) 218, 519, 790
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 1 cut(s) 702
BstACI GRCGYC 3 cut(s) 218, 519, 790
BstAUI TGTACA 1 cut(s) 730
BstBAI YACGTR 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 700, 815
BstC8I GCNNGC 3 cut(s) 249, 270, 292
BstDEI CTNAG 5 cut(s) 109, 362, 477, 662, 799
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 1 cut(s) 251
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 3 cut(s) 258, 283, 573
BstMAI GTCTC 2 cut(s) 107, 413
BstMBI GATC 3 cut(s) 255, 280, 570
BstMWI GCNNNNNNNGC 2 cut(s) 620, 719
BstNSI RCATGY 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 249
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 5 cut(s) 274, 347, 414, 815, 825
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 5 cut(s) 26, 191, 239, 374, 554
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 733
Cac8I GCNNGC 3 cut(s) 249, 270, 292
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 373, 409
CpoI CGGWCCG 1 cut(s) 409
CseI GACGC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 594, 731
CspI CGGWCCG 1 cut(s) 409
CviAII CATG 5 cut(s) 28, 291, 295, 742, 746
CviQI GTAC 2 cut(s) 594, 731
DdeI CTNAG 5 cut(s) 109, 362, 477, 662, 799
DpnI GATC 3 cut(s) 257, 282, 572
DpnII GATC 3 cut(s) 255, 280, 570
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 3 cut(s) 24, 237, 552
Eam1104I CTCTTC 1 cut(s) 702
EarI CTCTTC 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 783
Eco47I GGWCC 1 cut(s) 409
Eco72I CACGTG 1 cut(s) 331
Eco88I CYCGRG 4 cut(s) 182, 205, 375, 865
EcoRV GATATC 1 cut(s) 783
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 2 cut(s) 296, 747
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 5 cut(s) 31, 294, 298, 745, 749
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 5 cut(s) 27, 290, 294, 741, 745
Fnu4HI GCNGC 8 cut(s) 24, 234, 237, 288, 336, 428, 804, 839
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 8 cut(s) 24, 234, 237, 288, 336, 428, 804, 839
FspBI CTAG 2 cut(s) 794, 854
GlaI GCGC 1 cut(s) 324
GluI GCNGC 8 cut(s) 24, 234, 237, 288, 336, 428, 804, 839
GsuI CTGGAG 1 cut(s) 618
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 5 cut(s) 26, 191, 239, 374, 554
HgaI GACGC 1 cut(s) 226
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 3 cut(s) 218, 519, 790
Hin1II CATG 5 cut(s) 31, 294, 298, 745, 749
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 7 cut(s) 172, 203, 378, 389, 658, 692, 827
HphI GGTGA 2 cut(s) 310, 836
Hpy166II GTNNAC 2 cut(s) 318, 405
Hpy188I TCNGA 4 cut(s) 112, 196, 507, 651
Hpy188III TCNNGA 3 cut(s) 382, 786, 867
Hpy8I GTNNAC 2 cut(s) 318, 405
Hpy99I CGWCG 2 cut(s) 220, 524
HpyAV CCTTC 2 cut(s) 460, 508
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 3 cut(s) 330, 519, 790
HpyCH4V TGCA 7 cut(s) 290, 294, 430, 541, 655, 722, 745
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 719
HpyF3I CTNAG 5 cut(s) 109, 362, 477, 662, 799
HpySE526I ACGT 3 cut(s) 330, 519, 790
Hsp92I GRCGYC 3 cut(s) 218, 519, 790
Hsp92II CATG 5 cut(s) 31, 294, 298, 745, 749
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 3 cut(s) 255, 280, 570
LmnI GCTCC 3 cut(s) 163, 265, 330
Lsp1109I GCAGC 5 cut(s) 274, 347, 414, 815, 825
LweI GCATC 3 cut(s) 10, 36, 642
MaeI CTAG 2 cut(s) 794, 854
MaeII ACGT 3 cut(s) 330, 519, 790
MalI GATC 3 cut(s) 257, 282, 572
MboI GATC 3 cut(s) 255, 280, 570
MboII GAAGA 4 cut(s) 80, 320, 689, 804
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 2 cut(s) 215, 249
MlsI TGGCCA 1 cut(s) 554
MluCI AATT 2 cut(s) 508, 564
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 5 cut(s) 166, 197, 383, 387, 667
MmeI TCCRAC 2 cut(s) 435, 788
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 2 cut(s) 296, 747
MscI TGGCCA 1 cut(s) 554
MseI TTAA 3 cut(s) 51, 59, 354
MslI CAYNNNNRTG 2 cut(s) 44, 650
Msp20I TGGCCA 1 cut(s) 554
Mva1269I GAATGC 2 cut(s) 616, 625
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 620, 719
NcoI CCATGG 1 cut(s) 27
NdeII GATC 3 cut(s) 255, 280, 570
NlaIII CATG 5 cut(s) 31, 294, 298, 745, 749
NlaIV GGNNCC 1 cut(s) 282
NsiI ATGCAT 2 cut(s) 296, 747
NspI RCATGY 1 cut(s) 294
NspV TTCGAA 2 cut(s) 700, 815
PaeI GCATGC 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 205
PcsI WCGNNNNNNNCGW 3 cut(s) 212, 255, 336
PctI GAATGC 2 cut(s) 616, 625
PfeI GAWTC 2 cut(s) 692, 827
PkrI GCNGC 8 cut(s) 25, 235, 238, 289, 337, 429, 805, 840
PleI GAGTC 5 cut(s) 166, 197, 383, 386, 666
PmaCI CACGTG 1 cut(s) 331
PmlI CACGTG 1 cut(s) 331
PpsI GAGTC 5 cut(s) 166, 197, 383, 386, 666
Ppu21I YACGTR 1 cut(s) 331
PspCI CACGTG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 373, 409
PspXI VCTCGAGB 1 cut(s) 205
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 2 cut(s) 595, 732
RsaNI GTAC 2 cut(s) 594, 731
RseI CAYNNNNRTG 2 cut(s) 44, 650
Rsr2I CGGWCCG 1 cut(s) 409
RsrII CGGWCCG 1 cut(s) 409
SaqAI TTAA 3 cut(s) 51, 59, 354
SatI GCNGC 8 cut(s) 24, 234, 237, 288, 336, 428, 804, 839
Sau3AI GATC 3 cut(s) 255, 280, 570
Sau96I GGNCC 2 cut(s) 373, 409
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 5 cut(s) 166, 197, 383, 387, 667
SduI GDGCHC 2 cut(s) 215, 249
SfaNI GCATC 3 cut(s) 10, 36, 642
Sfr274I CTCGAG 1 cut(s) 205
SfuI TTCGAA 2 cut(s) 700, 815
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 44, 650
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
SphI GCATGC 1 cut(s) 294
Sse9I AATT 2 cut(s) 508, 564
SsiI CCGC 4 cut(s) 23, 233, 236, 251
SspMI CTAG 2 cut(s) 794, 854
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 3 cut(s) 333, 522, 793
TaqI TCGA 5 cut(s) 206, 215, 522, 700, 815
TaqII GACCGA 1 cut(s) 426
TasI AATT 2 cut(s) 508, 564
TatI WGTACW 2 cut(s) 593, 730
TauI GCSGC 3 cut(s) 26, 236, 239
TfiI GAWTC 2 cut(s) 692, 827
Tru1I TTAA 3 cut(s) 51, 59, 354
Tru9I TTAA 3 cut(s) 51, 59, 354
TseI GCWGC 5 cut(s) 287, 335, 427, 803, 838
TspGWI ACGGA 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 409
XceI RCATGY 1 cut(s) 294
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 2 cut(s) 794, 854
ZraI GACGTC 2 cut(s) 520, 791
ZrmI AGTACT 1 cut(s) 595
Zsp2I ATGCAT 2 cut(s) 296, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.