pycom08g05680

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002261
Physical Location & Seq
Reverse (-)
4304228 .. 4306161
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05680.1

Sequence Viewer

Length: 1332 bp
ATGGAGAAAGAGCTCCATCGAGCCGCCGTGCGTGGATCCGTCGACGCGCTTCTCGAACTACTCAGTGAAGACCCTCAAATCCTCAATAGGACAGCCCCATCTCTCTCCGACACGCCCTTACACGTAGCCTCGCTTCTGGGCCACTCTGCTTTCGCCAAAGAGTTACTGAGTCGGAACCCCGAACTCGCCGCCGAGCTGGACTCCCGCGGCTCTTCGCCTCTTCACCTGGCGGCTGCAAAAGGGTCAGCGGAGATAGTGAAAAATCTGGTGCGGGTCAACCCAGATCTGGGTTTGGTGCTGGATCGCGATGGGTTCACTCCCCTGCATCTGGGAGTGATCAAGGGGCGAGCTGCGGTTGTGGCCGAGTTGGCCCGAGTCAGACCGGCGGCGACTCGGGTTTTGACTCACGGAGGCGAGGGTGGGTTGCACTTGTGTGTGAAGCATCACAGGCTGGAGGTGTTGAAATTTTTGATGGACTGTATAGGGAGAGATGATGAGTTTGTGAATTGGAGGGATGGCGATGGTAACACAGTCTTACATGTTGCGGTGGCCAAGAAACAATTAGAGGTTATAAAGTATTTGCTGGCTAATACAAAGATAGATGTGAATGCCCAAAATGCGATTGGTTTCACCGCATTGGACGTCTTATCTCATAGCACAAGAGATTTAAGAGACTTGGAAATCAAACAGTCCCTTCAGAAAGCTGCAGCTCCAAGAACGAACAGGGCACAATCTGTTGCATTCGAGCCGGAAATGGATTCAGTTCGAGTCTCCAACTCGATCTCAATGCACCCGCAAATGTCGAAGAAAATGAGTGTAAAGGAAATAGTTAAGAAGAAGGACATTGATTGGTTGGGAAGGAAACGAAGCTCGTTAATGGTTGTGTCTTCCCTAATTGCGACAGTGGCATTTCAAGCTGCATTATCCCCTCCAGGCGGCGTATGGCAGGATGACTACTTGGTATACTCCAATGGCACCGCTGTGGAGCGTCCACACAACGCCGGGCAGTCTGTGATGGCAATTACAGAGCCAGTTCAATACGGACAGTTCATGATCTTCAACACGATTGCTTTCCTTGCATCATTGAGCATAATTCTACTGCTCGTGAGCGGATTGCCTATGAGGCGGAAGAGATGGATGTGGTTTCAGATGGTGATCATGTGGATTGCAATAACGGCACTATCGGGTACTTATTTTATTGGATTAATCTTCATGACACCGGACAGGAACCAGGGCAGCTATCTGTATTATGTGACTCAAATTTCTGTGCTGATATGGCTGGCGTTGATGGGGCTTGTGTTCATTGGTAACGTGATCCGGCTTGGTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

444

Amino Acids

48.8

Weight (kDa)

9.28

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 91 1.9e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 67 - 124 8.4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 138 - 206 2.8e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 142 - 195 4.7e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 168 - 215 2.4e-07 Ankyrin repeats (many copies)
Ank PF00023 174 - 206 2.3e-06 Ankyrin repeat
Ank_4 PF13637 179 - 214 1e-05 Ankyrin repeats (many copies)
PGG PF13962 283 - 404 2.8e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AatII GACGTC 1 cut(s) 645
AccB1I GGYRCC 1 cut(s) 974
AccBSI CCGCTC 1 cut(s) 1110
AccI GTMKAC 2 cut(s) 42, 963
AccII CGCG 3 cut(s) 47, 207, 306
AclWI GGATC 4 cut(s) 30, 43, 309, 1309
AcoI YGGCCR 2 cut(s) 360, 549
AcsI RAATTY 2 cut(s) 464, 1260
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 642
AfaI GTAC 1 cut(s) 1189
AfiI CCNNNNNNNGG 4 cut(s) 286, 287, 328, 935
AflIII ACRYGT 2 cut(s) 121, 538
AgsI TTSAA 4 cut(s) 463, 914, 1037, 1060
AjnI CCWGG 3 cut(s) 225, 931, 1230
AleI CACNNNNGTG 2 cut(s) 432, 980
AluBI AGCT 8 cut(s) 13, 196, 350, 704, 710, 870, 917, 1239
AluI AGCT 8 cut(s) 13, 196, 350, 704, 710, 870, 917, 1239
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 2 cut(s) 666, 775
AlwI GGATC 4 cut(s) 30, 43, 309, 1309
Ama87I CYCGRG 2 cut(s) 372, 393
AoxI GGCC 4 cut(s) 139, 360, 369, 549
ApeKI GCWGC 6 cut(s) 233, 350, 704, 707, 917, 1236
ApoI RAATTY 2 cut(s) 464, 1260
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
AseI ATTAAT 1 cut(s) 1205
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 139, 370
AsuC2I CCSGG 1 cut(s) 1003
AsuHPI GGTGA 3 cut(s) 215, 622, 1165
AvaI CYCGRG 2 cut(s) 372, 393
BaeGI GKGCMC 1 cut(s) 730
BalI TGGCCA 1 cut(s) 551
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 974
BanII GRGCYC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 117, 149
BauI CACGAG 1 cut(s) 1103
BbsI GAAGAC 2 cut(s) 75, 879
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 6 cut(s) 220, 337, 691, 719, 904, 1248
BceAI ACGGC 2 cut(s) 11, 1191
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 3 cut(s) 227, 933, 1232
BclI TGATCA 2 cut(s) 336, 1155
BcnI CCSGG 1 cut(s) 1003
BcoDI GTCTC 2 cut(s) 666, 775
BfmI CTRYAG 1 cut(s) 705
BglI GCCNNNNNGGC 2 cut(s) 368, 1123
BglII AGATCT 1 cut(s) 283
Bme1390I CCNGG 4 cut(s) 227, 933, 1003, 1232
BmeT110I CYCGRG 2 cut(s) 372, 393
BmgT120I GGNCC 2 cut(s) 139, 370
BmiI GGNNCC 4 cut(s) 37, 176, 976, 1229
BmrFI CCNGG 4 cut(s) 227, 933, 1003, 1232
BmsI GCATC 3 cut(s) 334, 451, 1088
BpiI GAAGAC 2 cut(s) 75, 879
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BpmI CTGGAG 2 cut(s) 473, 915
BpuMI CCSGG 1 cut(s) 1003
BsaAI YACGTR 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 1154
BsaHI GRCGYC 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 205, 1231
BsaWI WCCGGW 1 cut(s) 1219
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 287, 328, 935
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 1 cut(s) 1031
Bse8I GATNNNNATC 1 cut(s) 1154
BseBI CCWGG 3 cut(s) 227, 933, 1232
BseDI CCNNGG 2 cut(s) 205, 1231
BseGI GGATG 3 cut(s) 520, 955, 1143
BseJI GATNNNNATC 1 cut(s) 1154
BseLI CCNNNNNNNGG 4 cut(s) 286, 287, 328, 935
BseMII CTCAG 2 cut(s) 76, 158
BseNI ACTGG 1 cut(s) 1031
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 6 cut(s) 220, 337, 691, 719, 904, 1248
Bsh1236I CGCG 3 cut(s) 47, 207, 306
BshFI GGCC 4 cut(s) 141, 362, 371, 551
BshNI GGYRCC 1 cut(s) 974
BsiHKAI GWGCWC 1 cut(s) 15
BsiHKCI CYCGRG 2 cut(s) 372, 393
BsiSI CCGG 5 cut(s) 383, 749, 1002, 1220, 1318
BslFI GGGAC 1 cut(s) 676
BslI CCNNNNNNNGG 4 cut(s) 286, 287, 328, 935
BsmAI GTCTC 2 cut(s) 666, 775
BsmFI GGGAC 1 cut(s) 676
BsmI GAATGC 2 cut(s) 613, 740
BsnI GGCC 4 cut(s) 141, 362, 371, 551
BsoBI CYCGRG 2 cut(s) 372, 393
Bsp1286I GDGCHC 2 cut(s) 15, 730
Bsp143I GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
Bsp68I TCGCGA 1 cut(s) 306
BspANI GGCC 4 cut(s) 141, 362, 371, 551
BspCNI CTCAG 2 cut(s) 75, 159
BspFNI CGCG 3 cut(s) 47, 207, 306
BspHI TCATGA 2 cut(s) 1050, 1212
BspLI GGNNCC 4 cut(s) 37, 176, 976, 1229
BspMAI CTGCAG 1 cut(s) 709
BspPI GGATC 4 cut(s) 30, 43, 309, 1309
BspQI GCTCTTC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 1110
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 1 cut(s) 1031
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 205, 1231
BssMI GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
BssNAI GTATAC 1 cut(s) 964
BssNI GRCGYC 1 cut(s) 642
BssSI CACGAG 1 cut(s) 1103
Bst1107I GTATAC 1 cut(s) 964
Bst2BI CACGAG 1 cut(s) 1103
Bst2UI CCWGG 3 cut(s) 227, 933, 1232
Bst4CI ACNGT 5 cut(s) 479, 532, 690, 904, 1047
Bst6I CTCTTC 3 cut(s) 217, 225, 1124
BstACI GRCGYC 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 348, 585, 1281
BstDEI CTNAG 2 cut(s) 62, 167
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 3 cut(s) 520, 955, 1143
BstFNI CGCG 3 cut(s) 47, 207, 306
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 8 cut(s) 38, 286, 304, 339, 783, 1056, 1158, 1317
BstMAI GTCTC 2 cut(s) 666, 775
BstMBI GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
BstNI CCWGG 3 cut(s) 227, 933, 1232
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 4 cut(s) 225, 931, 1001, 1230
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 730
BstUI CGCG 3 cut(s) 47, 207, 306
BstV1I GCAGC 6 cut(s) 220, 337, 691, 719, 904, 1248
BstV2I GAAGAC 2 cut(s) 75, 879
BstX2I RGATCY 2 cut(s) 35, 283
BstYI RGATCY 2 cut(s) 35, 283
BstZ17I GTATAC 1 cut(s) 964
BsuRI GGCC 4 cut(s) 141, 362, 371, 551
BtgI CCRYGG 1 cut(s) 205
BtgZI GCGATG 2 cut(s) 321, 534
BtsCI GGATG 3 cut(s) 520, 955, 1143
BtsIMutI CAGTG 2 cut(s) 70, 909
BtuMI TCGCGA 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 348, 585, 1281
CciI TCATGA 2 cut(s) 1050, 1212
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 139, 370
Cfr42I CCGCGG 1 cut(s) 208
CseI GACGC 2 cut(s) 53, 977
Csp6I GTAC 1 cut(s) 1188
CviAII CATG 4 cut(s) 539, 1051, 1159, 1213
CviQI GTAC 1 cut(s) 1188
DdeI CTNAG 2 cut(s) 62, 167
DpnI GATC 8 cut(s) 37, 285, 303, 338, 782, 1055, 1157, 1316
DpnII GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
EaeI YGGCCR 2 cut(s) 360, 549
Eam1104I CTCTTC 3 cut(s) 217, 225, 1124
EarI CTCTTC 3 cut(s) 217, 225, 1124
EciI GGCGGA 1 cut(s) 1141
Ecl136II GAGCTC 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 2 cut(s) 372, 393
EcoICRI GAGCTC 1 cut(s) 13
EcoRII CCWGG 3 cut(s) 225, 931, 1230
EcoT38I GRGCYC 1 cut(s) 15
FaeI CATG 4 cut(s) 542, 1054, 1162, 1216
FaqI GGGAC 1 cut(s) 676
FatI CATG 4 cut(s) 538, 1050, 1158, 1212
FauI CCCGC 3 cut(s) 212, 264, 801
FbaI TGATCA 2 cut(s) 336, 1155
FblI GTMKAC 2 cut(s) 42, 963
FokI GGATG 3 cut(s) 527, 962, 1150
FriOI GRGCYC 1 cut(s) 15
GlaI GCGC 1 cut(s) 48
GsuI CTGGAG 2 cut(s) 473, 915
HaeIII GGCC 4 cut(s) 141, 362, 371, 551
HapII CCGG 5 cut(s) 383, 749, 1002, 1220, 1318
HgaI GACGC 2 cut(s) 53, 977
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 4 cut(s) 542, 1054, 1162, 1216
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 2 cut(s) 43, 277
HindII GTYRAC 2 cut(s) 43, 277
HinfI GANTC 8 cut(s) 169, 200, 375, 391, 403, 758, 768, 1255
HpaII CCGG 5 cut(s) 383, 749, 1002, 1220, 1318
HphI GGTGA 3 cut(s) 215, 622, 1165
Hpy166II GTNNAC 5 cut(s) 43, 277, 315, 964, 992
Hpy188I TCNGA 5 cut(s) 109, 174, 380, 699, 1149
Hpy188III TCNNGA 5 cut(s) 53, 305, 1051, 1105, 1213
Hpy8I GTNNAC 5 cut(s) 43, 277, 315, 964, 992
Hpy99I CGWCG 2 cut(s) 44, 47
HpyAV CCTTC 3 cut(s) 704, 832, 852
HpyCH4III ACNGT 5 cut(s) 479, 532, 690, 904, 1047
HpyCH4IV ACGT 3 cut(s) 123, 642, 1311
HpyCH4V TGCA 9 cut(s) 236, 325, 427, 707, 740, 790, 920, 1079, 1169
HpyF3I CTNAG 2 cut(s) 62, 167
HpySE526I ACGT 3 cut(s) 123, 642, 1311
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 4 cut(s) 542, 1054, 1162, 1216
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 336, 1155
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
LguI GCTCTTC 1 cut(s) 217
LmnI GCTCC 3 cut(s) 18, 715, 985
Lsp1109I GCAGC 6 cut(s) 220, 337, 691, 719, 904, 1248
LweI GCATC 3 cut(s) 334, 451, 1088
MaeII ACGT 3 cut(s) 123, 642, 1311
MaeIII GTNAC 4 cut(s) 162, 524, 1252, 1307
MalI GATC 8 cut(s) 37, 285, 303, 338, 782, 1055, 1157, 1316
MbiI CCGCTC 1 cut(s) 1110
MboI GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
MboII GAAGA 9 cut(s) 80, 204, 212, 817, 847, 879, 1048, 1141, 1201
MflI RGATCY 2 cut(s) 35, 283
MhlI GDGCHC 2 cut(s) 15, 730
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 7 cut(s) 464, 505, 560, 894, 1020, 1092, 1260
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 7 cut(s) 178, 194, 384, 385, 397, 777, 1249
MmeI TCCRAC 3 cut(s) 132, 152, 798
Mox20I TGGCCA 1 cut(s) 551
MscI TGGCCA 1 cut(s) 551
MseI TTAA 4 cut(s) 668, 831, 875, 1205
MslI CAYNNNNRTG 2 cut(s) 432, 980
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 3 cut(s) 207, 248, 980
MspI CCGG 5 cut(s) 383, 749, 1002, 1220, 1318
MspR9I CCNGG 4 cut(s) 227, 933, 1003, 1232
Mva1269I GAATGC 2 cut(s) 613, 740
MvaI CCWGG 3 cut(s) 227, 933, 1232
MvnI CGCG 3 cut(s) 47, 207, 306
NciI CCSGG 1 cut(s) 1003
NdeII GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
NlaIII CATG 4 cut(s) 542, 1054, 1162, 1216
NlaIV GGNNCC 4 cut(s) 37, 176, 976, 1229
NmeAIII GCCGAG 2 cut(s) 217, 388
NmuCI GTSAC 1 cut(s) 1252
NruI TCGCGA 1 cut(s) 306
NspI RCATGY 1 cut(s) 542
OliI CACNNNNGTG 2 cut(s) 432, 980
PagI TCATGA 2 cut(s) 1050, 1212
PciI ACATGT 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 613, 740
PfeI GAWTC 1 cut(s) 758
PleI GAGTC 7 cut(s) 177, 194, 383, 385, 397, 776, 1249
PpsI GAGTC 7 cut(s) 177, 194, 383, 385, 397, 776, 1249
Ppu21I YACGTR 1 cut(s) 124
PscI ACATGT 1 cut(s) 538
PshBI ATTAAT 1 cut(s) 1205
PsiI TTATAA 1 cut(s) 572
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 3 cut(s) 225, 931, 1230
PspGI CCWGG 3 cut(s) 225, 931, 1230
PspN4I GGNNCC 4 cut(s) 37, 176, 976, 1229
PspPI GGNCC 2 cut(s) 139, 370
PstI CTGCAG 1 cut(s) 709
PsuI RGATCY 2 cut(s) 35, 283
RruI TCGCGA 1 cut(s) 306
RsaI GTAC 1 cut(s) 1189
RsaNI GTAC 1 cut(s) 1188
RseI CAYNNNNRTG 2 cut(s) 432, 980
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 208
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 217
SaqAI TTAA 4 cut(s) 668, 831, 875, 1205
Sau3AI GATC 8 cut(s) 35, 283, 301, 336, 780, 1053, 1155, 1314
Sau96I GGNCC 2 cut(s) 139, 370
SchI GAGTC 7 cut(s) 178, 194, 384, 385, 397, 777, 1249
ScrFI CCNGG 4 cut(s) 227, 933, 1003, 1232
SduI GDGCHC 2 cut(s) 15, 730
SfaNI GCATC 3 cut(s) 334, 451, 1088
SfcI CTRYAG 1 cut(s) 705
SfiI GGCCNNNNNGGCC 1 cut(s) 368
Sfr303I CCGCGG 1 cut(s) 208
SgrBI CCGCGG 1 cut(s) 208
SgrDI CGTCGACG 1 cut(s) 41
SmiMI CAYNNNNRTG 2 cut(s) 432, 980
Sse9I AATT 7 cut(s) 464, 505, 560, 894, 1020, 1092, 1260
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 4 cut(s) 225, 931, 1001, 1230
TaaI ACNGT 5 cut(s) 479, 532, 690, 904, 1047
TaiI ACGT 3 cut(s) 126, 645, 1314
TaqI TCGA 7 cut(s) 19, 42, 54, 744, 766, 779, 803
TasI AATT 7 cut(s) 464, 505, 560, 894, 1020, 1092, 1260
TauI GCSGC 6 cut(s) 26, 191, 210, 233, 389, 939
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 4 cut(s) 668, 831, 875, 1205
Tru9I TTAA 4 cut(s) 668, 831, 875, 1205
TscAI CASTG 2 cut(s) 70, 909
TseFI GTSAC 1 cut(s) 1252
TseI GCWGC 6 cut(s) 233, 350, 704, 707, 917, 1236
Tsp45I GTSAC 1 cut(s) 1252
TspDTI ATGAA 3 cut(s) 1039, 1201, 1291
TspGWI ACGGA 3 cut(s) 28, 423, 1056
TspRI CASTG 2 cut(s) 70, 909
VspI ATTAAT 1 cut(s) 1205
XapI RAATTY 2 cut(s) 464, 1260
XceI RCATGY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 2 cut(s) 620, 939
XmiI GTMKAC 2 cut(s) 42, 963
ZraI GACGTC 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.