MD15G1095000.v1.1

galactose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003405
Physical Location & Seq
Reverse (-)
6640605 .. 6644030
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1095000.v1.1.491

Sequence Viewer

Length: 474 bp
ATGCACATGACCATCATGCCTAATAGTAACAAGGCCATTATGTTTGACGCTGCCGGTTTCGGTCCATCGGAGATCTCGTTGCTTCCTGGAGACTGTCGTCGGGTTTTCGATGAGAGGGATGAAACTGGAGAATACTATGAAGTGGATTGCTGGGCTCATGCTGTGGAATTTGACACTGGGACTGCAGCTATTAGGCCGCTTAAGATCTTGACAGACACATGGTGCTCATCTGGTGGATTATCAGCTAATGGCACTCTTGTGCAAACCGGTGGATGGGCTGTTGGAGGGAGGTCTGTGAGATACCTCTCCGGATGCAATACCTGTGACTGGGAGGAGCACCCCATGTCACTTTCTGCCTCAAGATGGTTCTCTACGCAACATATCTTGCCAAATGGCAATTTCATATTGGTCGGAGGCCGACGCATGTTCAATTACGAGTATATCCCTAAGGGGGGAGGTTCTAATGATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

158

Amino Acids

17.25

Weight (kDa)

5.09

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 1 - 151 1.8e-55 Glyoxal oxidase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 308
AciI CCGC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 222
AfiI CCNNNNNNNGG 5 cut(s) 273, 327, 363, 451, 452
AflII CTTAAG 1 cut(s) 200
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 1 cut(s) 430
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 2 cut(s) 188, 245
AluI AGCT 2 cut(s) 188, 245
Alw21I GWGCWC 2 cut(s) 227, 339
Alw26I GTCTC 1 cut(s) 84
Aor13HI TCCGGA 1 cut(s) 308
AoxI GGCC 3 cut(s) 33, 194, 415
ApeKI GCWGC 2 cut(s) 50, 185
ApoI RAATTY 1 cut(s) 167
AsiGI ACCGGT 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 62
AvaII GGWCC 1 cut(s) 62
AxyI CCTNAGG 1 cut(s) 447
BanII GRGCYC 1 cut(s) 157
Bbv12I GWGCWC 2 cut(s) 227, 339
BbvI GCAGC 2 cut(s) 37, 197
BccI CCATC 4 cut(s) 20, 73, 267, 357
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 183
BfrI CTTAAG 1 cut(s) 200
BglII AGATCT 2 cut(s) 72, 204
BisI GCNGC 3 cut(s) 51, 186, 197
BlsI GCNGC 3 cut(s) 52, 187, 198
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 2 cut(s) 186, 337
BmsI GCATC 1 cut(s) 302
BmuI ACTGGG 2 cut(s) 186, 337
BoxI GACNNNNGTC 1 cut(s) 96
BpmI CTGGAG 2 cut(s) 108, 147
BpuEI CTTGAG 1 cut(s) 343
BsaWI WCCGGW 2 cut(s) 266, 308
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 5 cut(s) 273, 327, 363, 451, 452
Bse118I RCCGGY 2 cut(s) 53, 266
Bse1I ACTGG 3 cut(s) 130, 181, 332
Bse21I CCTNAGG 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 308
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 124, 278, 317
BseLI CCNNNNNNNGG 5 cut(s) 273, 327, 363, 451, 452
BseNI ACTGG 3 cut(s) 130, 181, 332
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 37, 197
BseYI CCCAGC 1 cut(s) 150
BshFI GGCC 3 cut(s) 35, 196, 417
BshTI ACCGGT 1 cut(s) 266
BsiHKAI GWGCWC 2 cut(s) 227, 339
BsiSI CCGG 3 cut(s) 54, 267, 309
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 5 cut(s) 273, 327, 363, 451, 452
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 3 cut(s) 35, 196, 417
Bsp1286I GDGCHC 3 cut(s) 157, 227, 339
Bsp13I TCCGGA 1 cut(s) 308
Bsp143I GATC 2 cut(s) 72, 204
BspACI CCGC 1 cut(s) 197
BspANI GGCC 3 cut(s) 35, 196, 417
BspEI TCCGGA 1 cut(s) 308
BspMAI CTGCAG 1 cut(s) 187
BspTI CTTAAG 1 cut(s) 200
BsrFI RCCGGY 2 cut(s) 53, 266
BsrI ACTGG 3 cut(s) 130, 181, 332
BssAI RCCGGY 2 cut(s) 53, 266
BssMI GATC 2 cut(s) 72, 204
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 124, 278, 317
BstKTI GATC 2 cut(s) 75, 207
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 72, 204
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 37, 197
BstX2I RGATCY 2 cut(s) 72, 204
BstYI RGATCY 2 cut(s) 72, 204
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 35, 196, 417
BtsCI GGATG 3 cut(s) 124, 278, 317
BtsIMutI CAGTG 1 cut(s) 174
Cfr10I RCCGGY 2 cut(s) 53, 266
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 2 cut(s) 56, 429
CspAI ACCGGT 1 cut(s) 266
CviAII CATG 6 cut(s) 7, 16, 158, 219, 343, 424
CviJI RGCY 7 cut(s) 35, 155, 188, 196, 245, 278, 417
CviKI_1 RGCY 7 cut(s) 35, 155, 188, 196, 245, 278, 417
DdeI CTNAG 1 cut(s) 447
DpnI GATC 2 cut(s) 74, 206
DpnII GATC 2 cut(s) 72, 204
DraIII CACNNNGTG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 62
Eco81I CCTNAGG 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 157
FaeI CATG 6 cut(s) 10, 19, 161, 222, 346, 427
FaqI GGGAC 1 cut(s) 193
FatI CATG 6 cut(s) 6, 15, 157, 218, 342, 423
Fnu4HI GCNGC 3 cut(s) 51, 186, 197
FokI GGATG 3 cut(s) 131, 285, 324
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 3 cut(s) 51, 186, 197
GluI GCNGC 3 cut(s) 51, 186, 197
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 2 cut(s) 108, 147
HaeIII GGCC 3 cut(s) 35, 196, 417
HapII CCGG 3 cut(s) 54, 267, 309
HgaI GACGC 2 cut(s) 56, 429
Hin1II CATG 6 cut(s) 10, 19, 161, 222, 346, 427
HpaII CCGG 3 cut(s) 54, 267, 309
Hpy188I TCNGA 2 cut(s) 70, 413
Hpy188III TCNNGA 3 cut(s) 208, 309, 360
Hpy99I CGWCG 2 cut(s) 102, 423
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 4 cut(s) 4, 185, 262, 315
HpyF3I CTNAG 1 cut(s) 447
Hsp92II CATG 6 cut(s) 10, 19, 161, 222, 346, 427
Kpn2I TCCGGA 1 cut(s) 308
Kzo9I GATC 2 cut(s) 72, 204
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 2 cut(s) 37, 197
LweI GCATC 1 cut(s) 302
MaeIII GTNAC 3 cut(s) 26, 323, 345
MalI GATC 2 cut(s) 74, 206
MboI GATC 2 cut(s) 72, 204
MflI RGATCY 2 cut(s) 72, 204
MhlI GDGCHC 3 cut(s) 157, 227, 339
MluCI AATT 3 cut(s) 167, 397, 430
MmeI TCCRAC 2 cut(s) 262, 391
MnlI CCTC 8 cut(s) 108, 278, 282, 314, 325, 367, 407, 449
MroI TCCGGA 1 cut(s) 308
MseI TTAA 2 cut(s) 201, 472
MslI CAYNNNNRTG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 3 cut(s) 54, 267, 309
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
NdeII GATC 2 cut(s) 72, 204
NlaIII CATG 6 cut(s) 10, 19, 161, 222, 346, 427
NmuCI GTSAC 2 cut(s) 323, 345
NspI RCATGY 1 cut(s) 427
OliI CACNNNNGTG 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 85
PinAI ACCGGT 1 cut(s) 266
PkrI GCNGC 3 cut(s) 52, 187, 198
PshAI GACNNNNGTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 1 cut(s) 85
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 1 cut(s) 187
PsuI RGATCY 2 cut(s) 72, 204
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 201, 472
SatI GCNGC 3 cut(s) 51, 186, 197
Sau3AI GATC 2 cut(s) 72, 204
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 3 cut(s) 157, 227, 339
SetI ASST 6 cut(s) 190, 247, 293, 306, 323, 460
SfaNI GCATC 1 cut(s) 302
SfcI CTRYAG 1 cut(s) 183
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 200, 358
SmoI CTYRAG 2 cut(s) 200, 358
Sse9I AATT 3 cut(s) 167, 397, 430
SsiI CCGC 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 1 cut(s) 108
TaqII GACCGA 1 cut(s) 50
TasI AATT 3 cut(s) 167, 397, 430
TauI GCSGC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 201, 472
Tru9I TTAA 2 cut(s) 201, 472
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 323, 345
TseI GCWGC 2 cut(s) 50, 185
Tsp45I GTSAC 2 cut(s) 323, 345
TspDTI ATGAA 3 cut(s) 135, 153, 391
TspRI CASTG 1 cut(s) 181
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.