Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003405

Physical Location & Seq

Genomic Coordinates Reverse (-)

4545879 .. 4548461

Gene Structure 2583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G004860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGGCAGCCTTACACATTAAGCTTTTTTGCATCCTTCCTCTCCTCTTCGCCCTTGGCATCAATGCACAATGGCGAGGGCTTCCTTTTATCCCCAATCCGTTAGTTTCACACCATGGATTGGGTTTTAATCTTGACCCCAGCAACGGGGGCCAATTTCAGTTCTTTGACCCCAAGCCCGTCACTTTCAAAGCAGCCGGCGATAAACCTAAACCCAACGCCATCGCCGGGGGTGAACCCCAACCCGTCGCCGGGGGTGAACCCCAACCCGACGCAGGGGGTATACCCAAACGAGCGGTCATTAAAGCCGAAGATGCTAATGCTGTTCCCAACGCACAACCACAGCCGAACTTCCCTATTGGCAATAACCCGGGGGCGTGGGCACTTGTTTCCAATGACGCCGGTGTGTCTGCCATGCATCTAAATGTGCTTCCCAACAACAAGGCCATAATGTACGATGCCTCGGATTTCCATATATCGAACATCAAATTGCCCAACAATGAATGCCTTCCCTGGAACAATAATAAGGGCGAGACAGGAAACGATTGTTTTGCTCATGCAGTTGAATATGATCTTGACAAAAACACAGTTAGACCACTCAAGGTTCAGTTTGATGCATGGTGCTCATCGGGAGGGATTTTGCCCGATGGTAGGTTCGTCAGCACCGGTGGTTGGGTGAACGGATATAGAAGCGCTAGATATATAACCGCATGCAACGACGGCAAATGCGACTTCAAAGAGTACCAAAACGCATTTGCAGAAAATAGATGGTATGCAACTCAGATAACGCTAGCAGACGGTAGGGTGATTGTGGTTGGTGGCCGGAAAGCATACAGCATCGAGTACGTGCCACCAGAGGGACAAGAAAACAAGCAATCCATCTTCCTGCCTTTCCTTGACGAGACCACCGACATGGACGAGAACAATCTCTACCCGTTCGTGCACCAATCTACCGATGGCAACGTCTTCATCTTTGCCAACGACCGCTCCGTCCTCCTCAATCCCAAAACCAACAAAGTCATCAAGGAGTTCCCCAAACTCGACGGCGGATCCCGAAACTACCCGGCCTCCGGCATGTCGGCACTGCTCCCCATCGAGCTCAATGAGCAGAACCCCCCGGTCATCCCTGTCGACGTCCTCGTCTGCGGTGGCAATAAGCGAGACGCTTTCAAAATGTCCGCACAGAAACCACCAGTCTTCATCCCTGCCTTGGACGACTGCGGACGCCTCCGGATCACCGATCCTAATCCGGTCTGGGAAAAGGAGGTCATGCCCTCAAGACGTGTCATGGGAGACATGCTAAACCTTCCCACAGGTGAGCTCTTGATGATAAACGGTGCGATGGCTGGTGCCTCAGCTTGGTGGCAAGCTGAAGAACCCAACTTTACCCCTGTGATGTACAACCCCAAATTGCCCAATGGACAACGGTTCGTCAAGATGGCACCGACAACTATTGCAAGAATGTACCACTCCACCTCGGCAGTGCTCCCTAACGGTAAAATCCTCGTGGCAGGCAGTAATACTAACCCGGGCTATGACTTCAAGGCCAAGTACCCAACTGAGGTACGGGTGGAGACCTTCACCCCACCTTACATGGATAAGGCTTTGGACATCCACAGGCCGGAAATCAACGCCCAGGCCTCCGACAACAAGCTTAAGTACGGATCACCGTTCGGGGTCCAGTTCAAGCTGAACGAGCAAGACAAGCCTACAAAGGCTGACATCAAGGTCACCGTGTACGCTGTTCCTTTCACAACACATGGCTACTCCATGAACCAGAGGCTTGTTGTTTTGGCGAACACTGAGCTCAAGGCAGAGGGAGCTGGCTTATATCGTGTCACGGCGTTGGCCCCACCAACCGGAGCGGTGGCGCCACCAGGGTCCTACCTGCTGAATGTGGTTCACCGCGGAGTGCCGAGCACGGGAATGTGGGTGACGATTAACCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1929 Glyoxal_oxid_N

Protein Analysis

648

Amino Acids

70.82

Weight (kDa)

6.84

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyoxal_oxid_N	PF07250	138 - 530	5.6e-162	Glyoxal oxidase N-terminus
GO-like_E_set	PF09118	539 - 646	9.2e-26	Galactose oxidase-like, Early set domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67290 AT5G19580
fragaria_vesca	FvH4_1g05670 FvH4_2g35740
malus_domestica	MD02G1057700.v1.1 MD14G1235700.v1.1 MD15G1094900.v1.1 MD15G1095000.v1.1 MD15G1192100.v1.1
prunus_persica	Prupe.1G450000_v2.0.a1 Prupe.7G225100_v2.0.a1
pyrus_communis	pycom15g08750 pycom15g17200
rosa_chinensis	RchiOBHm_Chr2g0091311 RchiOBHm_Chr6g0306041
rosa_laevigata	RLG00000010829 RLG00000016210 RLG00000032216
rosa_multiflora	Rmu_sc0002270.1_g000045 Rmu_sc0025077.1_g000001
rosa_roxburghii	Rroxscaffold_2G00150220 Rroxscaffold_7G00162100
rosa_rugosa	Rorug02G0016200 Rorug06G0346600
rosa_samantha	Rh2AG061300 Rh2BG060400 Rh2CG062300 Rh2DG060900 Rh2DG061000 Rh6AG458600 Rh6BG442300 Rh6CG473000 Rh6DG459200 Rh7BG304600
rosa_wichuraiana	Rw2G004860 Rw6G039980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	986, 1136, 1724
AatII	GACGTC	1 cut(s)	1134
Acc36I	ACCTGC	1 cut(s)	1893
AccB1I	GGYRCC	3 cut(s)	1346, 1438, 1867
AccB7I	CCANNNNNTGG	1 cut(s)	118
AccBSI	CCGCTC	3 cut(s)	293, 984, 1862
AccI	GTMKAC	2 cut(s)	280, 1128
AccII	CGCG	1 cut(s)	1905
AccIII	TCCGGA	1 cut(s)	1227
AclWI	GGATC	5 cut(s)	1041, 1054, 1232, 1238, 1669
AcoI	YGGCCR	1 cut(s)	817
AcuI	CTGAAG	1 cut(s)	1389
AcyI	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
AfaI	GTAC	9 cut(s)	452, 740, 842, 1397, 1463, 1550, 1563, 1658, 1736
AfeI	AGCGCT	1 cut(s)	691
AflII	CTTAAG	1 cut(s)	1652
AflIII	ACRYGT	1 cut(s)	1279
AgeI	ACCGGT	1 cut(s)	662
AgsI	TTSAA	6 cut(s)	187, 563, 733, 1168, 1540, 1684
AjiI	CACGTC	1 cut(s)	1280
AjnI	CCWGG	3 cut(s)	509, 1632, 1873
AluBI	AGCT	9 cut(s)	22, 1096, 1318, 1355, 1367, 1651, 1687, 1804, 1820
AluI	AGCT	9 cut(s)	22, 1096, 1318, 1355, 1367, 1651, 1687, 1804, 1820
Alw21I	GWGCWC	7 cut(s)	623, 942, 1098, 1320, 1485, 1806, 1919
Alw26I	GTCTC	5 cut(s)	524, 893, 1152, 1284, 1565
Alw44I	GTGCAC	1 cut(s)	938
AlwI	GGATC	5 cut(s)	1041, 1054, 1232, 1238, 1669
Ama87I	CYCGRG	2 cut(s)	367, 1525
Aor13HI	TCCGGA	1 cut(s)	1227
Aor51HI	AGCGCT	1 cut(s)	691
AoxI	GGCC	8 cut(s)	148, 441, 817, 1062, 1542, 1616, 1635, 1845
ApaLI	GTGCAC	1 cut(s)	938
ApeKI	GCWGC	2 cut(s)	5, 191
ArsI	GACNNNNNNTTYG	2 cut(s)	279, 311
AsiGI	ACCGGT	1 cut(s)	662
Asp700I	GAANNNNTTC	1 cut(s)	504
AspLEI	GCGC	2 cut(s)	692, 1870
AspS9I	GGNCC	4 cut(s)	148, 1675, 1846, 1878
AsuC2I	CCSGG	8 cut(s)	226, 250, 368, 369, 1061, 1115, 1526, 1527
AsuNHI	GCTAGC	1 cut(s)	787
AvaI	CYCGRG	2 cut(s)	367, 1525
AvaII	GGWCC	2 cut(s)	1675, 1878
BaeGI	GKGCMC	2 cut(s)	382, 942
BamHI	GGATCC	1 cut(s)	1046
BanI	GGYRCC	3 cut(s)	1346, 1438, 1867
BanII	GRGCYC	3 cut(s)	1098, 1320, 1806
BauI	CACGAG	1 cut(s)	1502
BbsI	GAAGAC	2 cut(s)	955, 1186
Bbv12I	GWGCWC	7 cut(s)	623, 942, 1098, 1320, 1485, 1806, 1919
BbvCI	CCTCAGC	1 cut(s)	1351
BbvI	GCAGC	2 cut(s)	17, 203
BccI	CCATC	8 cut(s)	227, 638, 759, 884, 947, 1097, 1333, 1429
BceAI	ACGGC	3 cut(s)	733, 1057, 1854
BcgI	CGANNNNNNTGC	2 cut(s)	530, 564
BciT130I	CCWGG	3 cut(s)	511, 1634, 1875
BcnI	CCSGG	8 cut(s)	226, 250, 368, 369, 1061, 1115, 1526, 1527
BcoDI	GTCTC	5 cut(s)	524, 893, 1152, 1284, 1565
BfaI	CTAG	2 cut(s)	693, 788
BfoI	RGCGCY	2 cut(s)	693, 1871
BfrI	CTTAAG	1 cut(s)	1652
BfuAI	ACCTGC	1 cut(s)	1893
BisI	GCNGC	2 cut(s)	6, 192
BlsI	GCNGC	2 cut(s)	7, 193
Bme18I	GGWCC	2 cut(s)	1675, 1878
BmeT110I	CYCGRG	2 cut(s)	367, 1525
BmgBI	CACGTC	1 cut(s)	1280
BmgT120I	GGNCC	4 cut(s)	148, 1675, 1846, 1878
BmiI	GGNNCC	8 cut(s)	149, 1048, 1348, 1440, 1676, 1848, 1869, 1879
BmsI	GCATC	7 cut(s)	39, 66, 301, 424, 445, 601, 843
BmtI	GCTAGC	1 cut(s)	791
BpiI	GAAGAC	2 cut(s)	955, 1186
Bpu10I	CCTNAGC	1 cut(s)	1351
BpuEI	CTTGAG	3 cut(s)	581, 1258, 1790
BpuMI	CCSGG	8 cut(s)	226, 250, 368, 369, 1061, 1115, 1526, 1527
BsaAI	YACGTR	1 cut(s)	844
BsaBI	GATNNNNATC	1 cut(s)	1242
BsaHI	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
BsaI	GGTCTC	2 cut(s)	893, 1565
BsaWI	WCCGGW	4 cut(s)	662, 1227, 1246, 1856
BsaXI	ACNNNNNCTCC	4 cut(s)	968, 998, 1049, 1079
Bse118I	RCCGGY	3 cut(s)	194, 398, 662
Bse1I	ACTGG	3 cut(s)	1190, 1678, 1942
Bse8I	GATNNNNATC	1 cut(s)	1242
BseAI	TCCGGA	1 cut(s)	1227
BseBI	CCWGG	3 cut(s)	511, 1634, 1875
BseGI	GGATG	4 cut(s)	30, 1119, 1197, 1608
BseJI	GATNNNNATC	1 cut(s)	1242
BseMII	CTCAG	4 cut(s)	791, 1365, 1548, 1791
BseNI	ACTGG	3 cut(s)	1190, 1678, 1942
BseRI	GAGGAG	2 cut(s)	32, 983
BseSI	GKGCMC	2 cut(s)	382, 942
BseXI	GCAGC	2 cut(s)	17, 203
BseYI	CCCAGC	1 cut(s)	137
Bsh1236I	CGCG	1 cut(s)	1905
Bsh1285I	CGRYCG	1 cut(s)	982
BshFI	GGCC	8 cut(s)	150, 443, 819, 1064, 1544, 1618, 1637, 1847
BshNI	GGYRCC	3 cut(s)	1346, 1438, 1867
BshTI	ACCGGT	1 cut(s)	662
BsiEI	CGRYCG	1 cut(s)	982
BsiHKAI	GWGCWC	7 cut(s)	623, 942, 1098, 1320, 1485, 1806, 1919
BsiHKCI	CYCGRG	2 cut(s)	367, 1525
BslFI	GGGAC	1 cut(s)	870
BsmAI	GTCTC	5 cut(s)	524, 893, 1152, 1284, 1565
BsmBI	CGTCTC	1 cut(s)	1152
BsmFI	GGGAC	1 cut(s)	870
BsmI	GAATGC	1 cut(s)	506
BsnI	GGCC	8 cut(s)	150, 443, 819, 1064, 1544, 1618, 1637, 1847
Bso31I	GGTCTC	2 cut(s)	893, 1565
BsoBI	CYCGRG	2 cut(s)	367, 1525
Bsp1286I	GDGCHC	8 cut(s)	382, 623, 942, 1098, 1320, 1485, 1806, 1919
Bsp13I	TCCGGA	1 cut(s)	1227
Bsp1407I	TGTACA	1 cut(s)	1395
Bsp143I	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
Bsp19I	CCATGG	1 cut(s)	112
BspANI	GGCC	8 cut(s)	150, 443, 819, 1064, 1544, 1618, 1637, 1847
BspCNI	CTCAG	4 cut(s)	790, 1364, 1549, 1792
BspEI	TCCGGA	1 cut(s)	1227
BspFNI	CGCG	1 cut(s)	1905
BspLI	GGNNCC	8 cut(s)	149, 1048, 1348, 1440, 1676, 1848, 1869, 1879
BspMI	ACCTGC	1 cut(s)	1893
BspOI	GCTAGC	1 cut(s)	791
BspPI	GGATC	5 cut(s)	1041, 1054, 1232, 1238, 1669
BspT107I	GGYRCC	3 cut(s)	1346, 1438, 1867
BspTI	CTTAAG	1 cut(s)	1652
BspTNI	GGTCTC	2 cut(s)	893, 1565
BsrBI	CCGCTC	3 cut(s)	293, 984, 1862
BsrFI	RCCGGY	3 cut(s)	194, 398, 662
BsrGI	TGTACA	1 cut(s)	1395
BsrI	ACTGG	3 cut(s)	1190, 1678, 1942
BssAI	RCCGGY	3 cut(s)	194, 398, 662
BssMI	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
BssNAI	GTATAC	1 cut(s)	281
BssNI	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
BssSI	CACGAG	1 cut(s)	1502
BssT1I	CCWWGG	3 cut(s)	52, 112, 1206
Bst1107I	GTATAC	1 cut(s)	281
Bst2BI	CACGAG	1 cut(s)	1502
Bst2UI	CCWGG	3 cut(s)	511, 1634, 1875
Bst4CI	ACNGT	7 cut(s)	586, 797, 1334, 1425, 1493, 1668, 1732
Bst6I	CTCTTC	1 cut(s)	50
BstACI	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
BstAFI	CTTAAG	1 cut(s)	1652
BstAUI	TGTACA	1 cut(s)	1395
BstBAI	YACGTR	1 cut(s)	844
BstC8I	GCNNGC	6 cut(s)	196, 709, 789, 1365, 1510, 1822
BstDEI	CTNAG	4 cut(s)	777, 1351, 1557, 1800
BstDSI	CCRYGG	2 cut(s)	112, 1903
BstEII	GGTNACC	1 cut(s)	1726
BstF5I	GGATG	4 cut(s)	30, 1119, 1197, 1608
BstFNI	CGCG	1 cut(s)	1905
BstH2I	RGCGCY	2 cut(s)	693, 1871
BstHHI	GCGC	2 cut(s)	692, 1870
BstKTI	GATC	5 cut(s)	571, 1049, 1233, 1240, 1664
BstMAI	GTCTC	5 cut(s)	524, 893, 1152, 1284, 1565
BstMBI	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
BstMCI	CGRYCG	1 cut(s)	982
BstMWI	GCNNNNNNNGC	7 cut(s)	147, 311, 717, 1102, 1693, 1702, 1817
BstNI	CCWGG	3 cut(s)	511, 1634, 1875
BstNSI	RCATGY	3 cut(s)	711, 1075, 1297
BstPI	GGTNACC	1 cut(s)	1726
BstSLI	GKGCMC	2 cut(s)	382, 942
BstUI	CGCG	1 cut(s)	1905
BstV1I	GCAGC	2 cut(s)	17, 203
BstV2I	GAAGAC	2 cut(s)	955, 1186
BstX2I	RGATCY	1 cut(s)	1046
BstXI	CCANNNNNNTGG	1 cut(s)	910
BstYI	RGATCY	1 cut(s)	1046
BstZ17I	GTATAC	1 cut(s)	281
BsuRI	GGCC	8 cut(s)	150, 443, 819, 1064, 1544, 1618, 1637, 1847
BtgI	CCRYGG	2 cut(s)	112, 1903
BtgZI	GCGATG	2 cut(s)	205, 1352
BtrI	CACGTC	1 cut(s)	1280
BtsCI	GGATG	4 cut(s)	30, 1119, 1197, 1608
BtsI	GCAGTG	2 cut(s)	1079, 1485
BtsIMutI	CAGTG	3 cut(s)	1079, 1485, 1797
BveI	ACCTGC	1 cut(s)	1893
Cac8I	GCNNGC	6 cut(s)	196, 709, 789, 1365, 1510, 1822
CfoI	GCGC	2 cut(s)	692, 1870
Cfr10I	RCCGGY	3 cut(s)	194, 398, 662
Cfr13I	GGNCC	4 cut(s)	148, 1675, 1846, 1878
Cfr42I	CCGCGG	1 cut(s)	1906
Cfr9I	CCCGGG	2 cut(s)	367, 1525
CseI	GACGC	4 cut(s)	278, 404, 1169, 1230
Csp6I	GTAC	9 cut(s)	451, 739, 841, 1396, 1462, 1549, 1562, 1657, 1735
CspAI	ACCGGT	1 cut(s)	662
CviQI	GTAC	9 cut(s)	451, 739, 841, 1396, 1462, 1549, 1562, 1657, 1735
DdeI	CTNAG	4 cut(s)	777, 1351, 1557, 1800
DinI	GGCGCC	1 cut(s)	1869
DpnI	GATC	5 cut(s)	570, 1048, 1232, 1239, 1663
DpnII	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
DrdI	GACNNNNNNGTC	3 cut(s)	986, 1136, 1724
DseDI	GACNNNNNNGTC	3 cut(s)	986, 1136, 1724
EaeI	YGGCCR	1 cut(s)	817
Eam1104I	CTCTTC	1 cut(s)	50
EarI	CTCTTC	1 cut(s)	50
EciI	GGCGGA	1 cut(s)	1059
Ecl136II	GAGCTC	3 cut(s)	1096, 1318, 1804
Eco130I	CCWWGG	3 cut(s)	52, 112, 1206
Eco147I	AGGCCT	1 cut(s)	1637
Eco24I	GRGCYC	3 cut(s)	1098, 1320, 1806
Eco31I	GGTCTC	2 cut(s)	893, 1565
Eco47I	GGWCC	2 cut(s)	1675, 1878
Eco47III	AGCGCT	1 cut(s)	691
Eco53kI	GAGCTC	3 cut(s)	1096, 1318, 1804
Eco57I	CTGAAG	1 cut(s)	1389
Eco88I	CYCGRG	2 cut(s)	367, 1525
Eco91I	GGTNACC	1 cut(s)	1726
EcoICRI	GAGCTC	3 cut(s)	1096, 1318, 1804
EcoO109I	RGGNCCY	1 cut(s)	1878
EcoO65I	GGTNACC	1 cut(s)	1726
EcoRII	CCWGG	3 cut(s)	509, 1632, 1873
EcoT14I	CCWWGG	3 cut(s)	52, 112, 1206
EcoT22I	ATGCAT	2 cut(s)	417, 616
EcoT38I	GRGCYC	3 cut(s)	1098, 1320, 1806
EgeI	GGCGCC	1 cut(s)	1869
EheI	GGCGCC	1 cut(s)	1869
ErhI	CCWWGG	3 cut(s)	52, 112, 1206
Esp3I	CGTCTC	1 cut(s)	1152
FaqI	GGGAC	1 cut(s)	870
FblI	GTMKAC	2 cut(s)	280, 1128
Fnu4HI	GCNGC	2 cut(s)	6, 192
FokI	GGATG	4 cut(s)	17, 1106, 1184, 1595
FriOI	GRGCYC	3 cut(s)	1098, 1320, 1806
Fsp4HI	GCNGC	2 cut(s)	6, 192
FspBI	CTAG	2 cut(s)	693, 788
GlaI	GCGC	2 cut(s)	691, 1869
GluI	GCNGC	2 cut(s)	6, 192
GsaI	CCCAGC	1 cut(s)	141
HaeII	RGCGCY	2 cut(s)	693, 1871
HaeIII	GGCC	8 cut(s)	150, 443, 819, 1064, 1544, 1618, 1637, 1847
HgaI	GACGC	4 cut(s)	278, 404, 1169, 1230
HhaI	GCGC	2 cut(s)	692, 1870
Hin1I	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
Hin6I	GCGC	2 cut(s)	690, 1868
HinP1I	GCGC	2 cut(s)	690, 1868
HincII	GTYRAC	1 cut(s)	1129
HindII	GTYRAC	1 cut(s)	1129
HindIII	AAGCTT	2 cut(s)	20, 1649
Hpy166II	GTNNAC	8 cut(s)	233, 257, 281, 676, 940, 1129, 1735, 1900
Hpy188I	TCNGA	3 cut(s)	463, 780, 1642
Hpy188III	TCNNGA	8 cut(s)	131, 572, 627, 1050, 1228, 1275, 1321, 1432
Hpy8I	GTNNAC	8 cut(s)	233, 257, 281, 676, 940, 1129, 1735, 1900
Hpy99I	CGWCG	5 cut(s)	248, 272, 719, 1043, 1133
HpyAV	CCTTC	4 cut(s)	44, 515, 1313, 1585
HpyCH4III	ACNGT	7 cut(s)	586, 797, 1334, 1425, 1493, 1668, 1732
HpyCH4IV	ACGT	4 cut(s)	843, 960, 1131, 1279
HpyF10VI	GCNNNNNNNGC	7 cut(s)	147, 311, 717, 1102, 1693, 1702, 1817
HpyF3I	CTNAG	4 cut(s)	777, 1351, 1557, 1800
HpySE526I	ACGT	4 cut(s)	843, 960, 1131, 1279
Hsp92I	GRCGYC	4 cut(s)	396, 1131, 1222, 1868
HspAI	GCGC	2 cut(s)	690, 1868
KasI	GGCGCC	1 cut(s)	1867
Kpn2I	TCCGGA	1 cut(s)	1227
KroI	GCCGGC	1 cut(s)	194
KroNI	GCCGGC	1 cut(s)	196
KspI	CCGCGG	1 cut(s)	1906
Kzo9I	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
LmnI	GCTCC	5 cut(s)	989, 1089, 1488, 1817, 1859
Lsp1109I	GCAGC	2 cut(s)	17, 203
LweI	GCATC	7 cut(s)	39, 66, 301, 424, 445, 601, 843
MaeI	CTAG	2 cut(s)	693, 788
MaeII	ACGT	4 cut(s)	843, 960, 1131, 1279
MaeIII	GTNAC	4 cut(s)	178, 1726, 1834, 1930
MalI	GATC	5 cut(s)	570, 1048, 1232, 1239, 1663
MbiI	CCGCTC	3 cut(s)	293, 984, 1862
MboI	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
MboII	GAAGA	6 cut(s)	37, 320, 871, 955, 1186, 1382
MflI	RGATCY	1 cut(s)	1046
MhlI	GDGCHC	8 cut(s)	382, 623, 942, 1098, 1320, 1485, 1806, 1919
MluCI	AATT	3 cut(s)	152, 485, 1406
Mly113I	GGCGCC	1 cut(s)	1868
MmeI	TCCRAC	1 cut(s)	1665
Mph1103I	ATGCAT	2 cut(s)	417, 616
MroI	TCCGGA	1 cut(s)	1227
MroNI	GCCGGC	1 cut(s)	194
MroXI	GAANNNNTTC	1 cut(s)	504
MseI	TTAA	5 cut(s)	18, 126, 300, 1653, 1938
MslI	CAYNNNNRTG	3 cut(s)	420, 908, 1922
MspA1I	CMGCKG	1 cut(s)	1905
MspCI	CTTAAG	1 cut(s)	1652
Mva1269I	GAATGC	1 cut(s)	506
MvaI	CCWGG	3 cut(s)	511, 1634, 1875
MvnI	CGCG	1 cut(s)	1905
MwoI	GCNNNNNNNGC	7 cut(s)	147, 311, 717, 1102, 1693, 1702, 1817
NaeI	GCCGGC	1 cut(s)	196
NarI	GGCGCC	1 cut(s)	1868
NciI	CCSGG	8 cut(s)	226, 250, 368, 369, 1061, 1115, 1526, 1527
NcoI	CCATGG	1 cut(s)	112
NdeII	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
NgoMIV	GCCGGC	1 cut(s)	194
NheI	GCTAGC	1 cut(s)	787
NlaIV	GGNNCC	8 cut(s)	149, 1048, 1348, 1440, 1676, 1848, 1869, 1879
NmeAIII	GCCGAG	2 cut(s)	1454, 1938
NmuCI	GTSAC	4 cut(s)	178, 1726, 1834, 1930
NsiI	ATGCAT	2 cut(s)	417, 616
NspI	RCATGY	3 cut(s)	711, 1075, 1297
PaeI	GCATGC	1 cut(s)	711
PceI	AGGCCT	1 cut(s)	1637
PcsI	WCGNNNNNNNCGW	4 cut(s)	723, 903, 984, 1134
PctI	GAATGC	1 cut(s)	506
PdiI	GCCGGC	1 cut(s)	196
PdmI	GAANNNNTTC	1 cut(s)	504
PflMI	CCANNNNNTGG	1 cut(s)	118
PinAI	ACCGGT	1 cut(s)	662
PkrI	GCNGC	2 cut(s)	7, 193
PluTI	GGCGCC	1 cut(s)	1871
Ppu21I	YACGTR	1 cut(s)	844
PpuMI	RGGWCCY	1 cut(s)	1878
Psp124BI	GAGCTC	3 cut(s)	1098, 1320, 1806
Psp5II	RGGWCCY	1 cut(s)	1878
Psp6I	CCWGG	3 cut(s)	509, 1632, 1873
PspEI	GGTNACC	1 cut(s)	1726
PspFI	CCCAGC	1 cut(s)	137
PspGI	CCWGG	3 cut(s)	509, 1632, 1873
PspN4I	GGNNCC	8 cut(s)	149, 1048, 1348, 1440, 1676, 1848, 1869, 1879
PspPI	GGNCC	4 cut(s)	148, 1675, 1846, 1878
PspPPI	RGGWCCY	1 cut(s)	1878
PsrI	GAACNNNNNNTAC	2 cut(s)	911, 943
PsuI	RGATCY	1 cut(s)	1046
RsaI	GTAC	9 cut(s)	452, 740, 842, 1397, 1463, 1550, 1563, 1658, 1736
RsaNI	GTAC	9 cut(s)	451, 739, 841, 1396, 1462, 1549, 1562, 1657, 1735
RseI	CAYNNNNRTG	3 cut(s)	420, 908, 1922
SacI	GAGCTC	3 cut(s)	1098, 1320, 1806
SacII	CCGCGG	1 cut(s)	1906
SalI	GTCGAC	1 cut(s)	1127
SaqAI	TTAA	5 cut(s)	18, 126, 300, 1653, 1938
SatI	GCNGC	2 cut(s)	6, 192
Sau3AI	GATC	5 cut(s)	568, 1046, 1230, 1237, 1661
Sau96I	GGNCC	4 cut(s)	148, 1675, 1846, 1878
SduI	GDGCHC	8 cut(s)	382, 623, 942, 1098, 1320, 1485, 1806, 1919
SfaNI	GCATC	7 cut(s)	39, 66, 301, 424, 445, 601, 843
SfoI	GGCGCC	1 cut(s)	1869
Sfr303I	CCGCGG	1 cut(s)	1906
SgrAI	CRCCGGYG	2 cut(s)	398, 662
SgrBI	CCGCGG	1 cut(s)	1906
SinI	GGWCC	2 cut(s)	1675, 1878
SmaI	CCCGGG	2 cut(s)	369, 1527
SmiMI	CAYNNNNRTG	3 cut(s)	420, 908, 1922
SmlI	CTYRAG	4 cut(s)	596, 1273, 1652, 1805
SmoI	CTYRAG	4 cut(s)	596, 1273, 1652, 1805
SphI	GCATGC	1 cut(s)	711
Sse9I	AATT	3 cut(s)	152, 485, 1406
SseBI	AGGCCT	1 cut(s)	1637
SspDI	GGCGCC	1 cut(s)	1867
SspMI	CTAG	2 cut(s)	693, 788
SstI	GAGCTC	3 cut(s)	1098, 1320, 1806
StuI	AGGCCT	1 cut(s)	1637
StyI	CCWWGG	3 cut(s)	52, 112, 1206
TaaI	ACNGT	7 cut(s)	586, 797, 1334, 1425, 1493, 1668, 1732
TaiI	ACGT	4 cut(s)	846, 963, 1134, 1282
TaqI	TCGA	5 cut(s)	476, 837, 1038, 1092, 1128
TasI	AATT	3 cut(s)	152, 485, 1406
TatI	WGTACW	1 cut(s)	1395
Tru1I	TTAA	5 cut(s)	18, 126, 300, 1653, 1938
Tru9I	TTAA	5 cut(s)	18, 126, 300, 1653, 1938
TscAI	CASTG	3 cut(s)	1086, 1485, 1804
TseFI	GTSAC	4 cut(s)	178, 1726, 1834, 1930
TseI	GCWGC	2 cut(s)	5, 191
Tsp45I	GTSAC	4 cut(s)	178, 1726, 1834, 1930
TspDTI	ATGAA	4 cut(s)	513, 955, 1186, 1784
TspGWI	ACGGA	4 cut(s)	87, 693, 976, 1674
TspMI	CCCGGG	2 cut(s)	367, 1525
TspRI	CASTG	3 cut(s)	1086, 1485, 1804
Van91I	CCANNNNNTGG	1 cut(s)	118
Vha464I	CTTAAG	1 cut(s)	1652
VneI	GTGCAC	1 cut(s)	938
VpaK11BI	GGWCC	2 cut(s)	1675, 1878
XceI	RCATGY	3 cut(s)	711, 1075, 1297
XcmI	CCANNNNNNNNNTGG	1 cut(s)	950
XmaI	CCCGGG	2 cut(s)	367, 1525
XmiI	GTMKAC	2 cut(s)	280, 1128
XmnI	GAANNNNTTC	1 cut(s)	504
XspI	CTAG	2 cut(s)	693, 788
ZraI	GACGTC	1 cut(s)	1132
Zsp2I	ATGCAT	2 cut(s)	417, 616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.