MD15G1148500.v1.1

CDK-activating kinase assembly factor MAT1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001457
Physical Location & Seq
Forward (+)
10986035 .. 10988742
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1148500.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGTGGTTACCAATTCTAATTCCCAGAACAGGGAGATTGTGGTCAGGAAGAGGATTGCAAGCATATTCAATAAACGAGAAGAGGATTTTTCTTCCTTGAGAGAATACAATGATTACTTGGAGGAAGTGGAGGACATGACATTTGACTTGATCGAAGGAATAGATGTTCCTGCTATTGAAGCGAAAATTGCCAAGTACCAGGAAGAAAATGCCGAACAAATAATGATTAACAGAGCTCGTAAGGCTGAAGAGCTTGCTGCAGCTCTCGCAGCAAGCAAGGGACATCCCATACAAAATGAAACTGATGCGGGTCTGAGCCAAGGCTCACAAGCAGGATTTGGTGCTGGTACACAGGGCCAGTATGCTCCTACAGTTTCAGGGCAACCACGTCCAACCGGCGGCATGGGTCCACAACCACTACCACTTGGAGGAGGGGGGCATGATCTGCACGGATACGCTGTTGACGATGAAGAAATGGTGAAGCTGCGAGCAGAGAAGGGTGGTCGGGCAGGAGGGTGGAGTGGAGAGATAAGCAGGAAGAGGGCACTTGAAGAAGCCCTTGGTAGCATTTGGATTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko03420 map03022 map03420
Pfam Domains
Protein Families
MAT1

Protein Analysis

194

Amino Acids

20.89

Weight (kDa)

4.93

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAT1 PF06391 10 - 88 6.1e-30 CDK-activating kinase assembly factor MAT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 308, 399
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 2 cut(s) 197, 349
AfiI CCNNNNNNNGG 4 cut(s) 30, 31, 398, 428
AgsI TTSAA 3 cut(s) 70, 179, 551
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 1 cut(s) 198
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 4 cut(s) 236, 253, 263, 484
AluI AGCT 4 cut(s) 236, 253, 263, 484
Alw21I GWGCWC 1 cut(s) 238
AoxI GGCC 1 cut(s) 355
ApeKI GCWGC 4 cut(s) 257, 260, 269, 484
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
AspS9I GGNCC 2 cut(s) 355, 407
AsuHPI GGTGA 1 cut(s) 490
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 4 cut(s) 244, 272, 281, 471
BciT130I CCWGG 1 cut(s) 200
BciVI GTATCC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 258, 369
BfuI GTATCC 1 cut(s) 446
BisI GCNGC 5 cut(s) 258, 261, 270, 400, 485
BlsI GCNGC 5 cut(s) 259, 262, 271, 401, 486
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 407
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 355, 407
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 295
BpuEI CTTGAG 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 319, 559
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 31, 398, 428
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 319, 559
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 4 cut(s) 30, 31, 398, 428
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 1 cut(s) 444
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 4 cut(s) 244, 272, 281, 471
BsgI GTGCAG 1 cut(s) 431
BshFI GGCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 4 cut(s) 30, 31, 398, 428
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 1 cut(s) 357
Bsp1286I GDGCHC 2 cut(s) 238, 547
Bsp143I GATC 2 cut(s) 150, 442
BspACI CCGC 2 cut(s) 308, 399
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 408
BspMAI CTGCAG 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 319, 559
BssMI GATC 2 cut(s) 150, 442
BssT1I CCWWGG 2 cut(s) 319, 559
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 4 cut(s) 44, 75, 243, 533
BstAPI GCANNNNNTGC 1 cut(s) 445
BstC8I GCNNGC 4 cut(s) 61, 255, 274, 489
BstDEI CTNAG 1 cut(s) 314
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 2 cut(s) 153, 445
BstMBI GATC 2 cut(s) 150, 442
BstMWI GCNNNNNNNGC 6 cut(s) 179, 188, 242, 266, 269, 445
BstNI CCWGG 1 cut(s) 200
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 2 cut(s) 258, 369
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 4 cut(s) 244, 272, 281, 471
BsuI GTATCC 1 cut(s) 446
BsuRI GGCC 1 cut(s) 357
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 1 cut(s) 283
Cac8I GCNNGC 4 cut(s) 61, 255, 274, 489
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 355, 407
Csp6I GTAC 2 cut(s) 196, 348
CviAII CATG 3 cut(s) 136, 403, 440
CviJI RGCY 9 cut(s) 236, 245, 253, 263, 318, 324, 357, 484, 557
CviKI_1 RGCY 9 cut(s) 236, 245, 253, 263, 318, 324, 357, 484, 557
CviQI GTAC 2 cut(s) 196, 348
DdeI CTNAG 1 cut(s) 314
DpnI GATC 2 cut(s) 152, 444
DpnII GATC 2 cut(s) 150, 442
Eam1104I CTCTTC 4 cut(s) 44, 75, 243, 533
EarI CTCTTC 4 cut(s) 44, 75, 243, 533
Ecl136II GAGCTC 1 cut(s) 236
Eco130I CCWWGG 2 cut(s) 319, 559
Eco24I GRGCYC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 267
Eco91I GGTNACC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 236
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 319, 559
EcoT38I GRGCYC 1 cut(s) 238
ErhI CCWWGG 2 cut(s) 319, 559
FaeI CATG 3 cut(s) 139, 406, 443
FaiI YATR 6 cut(s) 65, 137, 290, 363, 404, 441
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 1 cut(s) 294
FatI CATG 3 cut(s) 135, 402, 439
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 5 cut(s) 258, 261, 270, 400, 485
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 5 cut(s) 258, 261, 270, 400, 485
GluI GCNGC 5 cut(s) 258, 261, 270, 400, 485
HaeIII GGCC 1 cut(s) 357
HapII CCGG 1 cut(s) 396
Hin1II CATG 3 cut(s) 139, 406, 443
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HpaII CCGG 1 cut(s) 396
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 3 cut(s) 350, 410, 463
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 3 cut(s) 350, 410, 463
HpyAV CCTTC 2 cut(s) 149, 490
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 3 cut(s) 59, 260, 448
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 188, 242, 266, 269, 445
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 139, 406, 443
Kzo9I GATC 2 cut(s) 150, 442
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 4 cut(s) 244, 272, 281, 471
LweI GCATC 1 cut(s) 295
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 1 cut(s) 7
MalI GATC 2 cut(s) 152, 444
MboI GATC 2 cut(s) 150, 442
MboII GAAGA 8 cut(s) 61, 84, 92, 215, 260, 482, 550, 563
MhlI GDGCHC 2 cut(s) 238, 547
MluCI AATT 3 cut(s) 13, 19, 186
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 8 cut(s) 45, 76, 115, 124, 422, 425, 506, 534
MseI TTAA 1 cut(s) 228
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 6 cut(s) 179, 188, 242, 266, 269, 445
NdeII GATC 2 cut(s) 150, 442
NlaIII CATG 3 cut(s) 139, 406, 443
NlaIV GGNNCC 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PkrI GCNGC 5 cut(s) 259, 262, 271, 401, 486
Psp124BI GAGCTC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 198
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 2 cut(s) 355, 407
PstI CTGCAG 1 cut(s) 262
RsaI GTAC 2 cut(s) 197, 349
RsaNI GTAC 2 cut(s) 196, 348
SacI GAGCTC 1 cut(s) 238
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 5 cut(s) 258, 261, 270, 400, 485
Sau3AI GATC 2 cut(s) 150, 442
Sau96I GGNCC 2 cut(s) 355, 407
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 238, 547
SetI ASST 5 cut(s) 238, 255, 265, 391, 486
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 258, 369
SinI GGWCC 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
Sse9I AATT 3 cut(s) 13, 19, 186
SsiI CCGC 2 cut(s) 308, 399
SstI GAGCTC 1 cut(s) 238
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 319, 559
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 153
TasI AATT 3 cut(s) 13, 19, 186
TauI GCSGC 1 cut(s) 402
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 4 cut(s) 257, 260, 269, 484
TspDTI ATGAA 2 cut(s) 312, 483
TspGWI ACGGA 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.