MD15G1202100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009948
Physical Location & Seq
Forward (+)
16096859 .. 16100335
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1202100.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAGGAAGCTGAGGAGAAAATTGAGAAATTCACAGGACGACGACTTCTCTCTACCCACTTGGGACGATCCTTCCCTCAACGATTCTCGCCCCATGGATACTCAAGAGCAAGAGGAGTTGGTTCGTGAAATGGAGAAGAGTCAAGCTCAACAGAGTCGGTTATGGAGGGTTTTGTTTGCATTGCTTCTCTTTTGTTTCGCGGTGTTTCTGTTGTACTCAATCTGTCAGCAGGTTCTGTCTCCTTGGGAATTGCATTATCATGCTTATTTCATGGAAGAGATGCTCTCATGGATGATTATATCTGCAGATTGGCTAGCTGTTGTAGCATATTCATCAGCTATCATAGGATTGCTTCATGATTCAAAGCATCACCGCCAATGGATTTGGTACTCACTCTTTACTGGCATTGTACTTGCGGCTTTCTGGCTGTATTACATGTTGAGATTGCCAAGGTTCCGCTGGGATGTAATCTGGCTTCCATTTGGACCTCTAAGCGGAGCAGCACTGGCTCTGTACGTTGATCATCTGCTAACTGAGTCATCAGAAGAGATAAGAAAGCTTCGAGGCTATATGTATGCTTACAAAGCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

23.56

Weight (kDa)

6.3

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 220
AccII CGCG 1 cut(s) 200
AciI CCGC 5 cut(s) 200, 373, 416, 457, 495
AclWI GGATC 1 cut(s) 62
AcsI RAATTY 1 cut(s) 28
AfaI GTAC 4 cut(s) 215, 389, 411, 515
AfiI CCNNNNNNNGG 1 cut(s) 494
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 363
AluBI AGCT 7 cut(s) 10, 146, 317, 338, 559, 587, 591
AluI AGCT 7 cut(s) 10, 146, 317, 338, 559, 587, 591
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 235
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 362
AsuNHI GCTAGC 2 cut(s) 313, 587
AvaII GGWCC 1 cut(s) 485
BarI GAAGNNNNNNTAC 2 cut(s) 459, 491
BbvCI CCTCAGC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 512
BciVI GTATCC 1 cut(s) 91
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 2 cut(s) 314, 588
BfmI CTRYAG 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 220
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 2 cut(s) 417, 501
BlsI GCNGC 2 cut(s) 418, 502
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 455
BmsI GCATC 2 cut(s) 270, 376
BmtI GCTAGC 2 cut(s) 317, 591
BplI GAGNNNNNCTC 4 cut(s) 130, 162, 269, 301
Bpu10I CCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 93, 242, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse1I ACTGG 2 cut(s) 406, 510
Bse3DI GCAATG 1 cut(s) 179
BseDI CCNNGG 3 cut(s) 93, 242, 449
BseGI GGATG 2 cut(s) 297, 469
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 179
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 406, 510
BseRI GAGGAG 2 cut(s) 28, 128
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 77
Bsp143I GATC 2 cut(s) 67, 520
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 5 cut(s) 200, 373, 416, 457, 495
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 1 cut(s) 200
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 220
BspOI GCTAGC 2 cut(s) 317, 591
BspPI GGATC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 179
BsrI ACTGG 2 cut(s) 406, 510
BssECI CCNNGG 3 cut(s) 93, 242, 449
BssMI GATC 2 cut(s) 67, 520
BssT1I CCWWGG 3 cut(s) 93, 242, 449
Bst6I CTCTTC 3 cut(s) 131, 270, 540
BstC8I GCNNGC 2 cut(s) 315, 589
BstDEI CTNAG 3 cut(s) 11, 491, 534
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 297, 469
BstFNI CGCG 1 cut(s) 200
BstKTI GATC 2 cut(s) 70, 523
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 2 cut(s) 67, 520
BstMWI GCNNNNNNNGC 3 cut(s) 323, 506, 584
BstNSI RCATGY 1 cut(s) 439
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 512
BsuI GTATCC 1 cut(s) 91
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 297, 469
BtsIMutI CAGTG 1 cut(s) 503
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 2 cut(s) 315, 589
CaiI CAGNNNCTG 1 cut(s) 235
CciI TCATGA 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 4 cut(s) 214, 388, 410, 514
CviAII CATG 6 cut(s) 94, 260, 271, 288, 356, 436
CviQI GTAC 4 cut(s) 214, 388, 410, 514
DdeI CTNAG 3 cut(s) 11, 491, 534
DpnI GATC 2 cut(s) 69, 522
DpnII GATC 2 cut(s) 67, 520
Eam1104I CTCTTC 3 cut(s) 131, 270, 540
EarI CTCTTC 3 cut(s) 131, 270, 540
Eco130I CCWWGG 3 cut(s) 93, 242, 449
Eco47I GGWCC 1 cut(s) 485
EcoT14I CCWWGG 3 cut(s) 93, 242, 449
ErhI CCWWGG 3 cut(s) 93, 242, 449
FaeI CATG 6 cut(s) 97, 263, 274, 291, 359, 439
FaqI GGGAC 1 cut(s) 77
FatI CATG 6 cut(s) 93, 259, 270, 287, 355, 435
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 2 cut(s) 417, 501
FokI GGATG 2 cut(s) 304, 476
Fsp4HI GCNGC 2 cut(s) 417, 501
FspBI CTAG 2 cut(s) 314, 588
GluI GCNGC 2 cut(s) 417, 501
GsaI CCCAGC 1 cut(s) 463
Hin1II CATG 6 cut(s) 97, 263, 274, 291, 359, 439
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 5 cut(s) 83, 139, 154, 359, 536
HphI GGTGA 1 cut(s) 362
Hpy188I TCNGA 1 cut(s) 544
Hpy188III TCNNGA 3 cut(s) 104, 125, 356
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 81
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 3 cut(s) 179, 253, 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 506, 584
HpyF3I CTNAG 3 cut(s) 11, 491, 534
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 6 cut(s) 97, 263, 274, 291, 359, 439
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 2 cut(s) 67, 520
LmnI GCTCC 1 cut(s) 497
LpnPI CCDG 7 cut(s) 20, 215, 387, 409, 445, 457, 491
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 270, 376
MaeI CTAG 2 cut(s) 314, 588
MaeII ACGT 1 cut(s) 516
MalI GATC 2 cut(s) 69, 522
MboI GATC 2 cut(s) 67, 520
MboII GAAGA 3 cut(s) 148, 287, 557
MluCI AATT 3 cut(s) 20, 28, 248
MlyI GAGTC 3 cut(s) 148, 163, 545
MnlI CCTC 6 cut(s) 6, 86, 106, 159, 498, 557
MslI CAYNNNNRTG 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 459
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 323, 506, 584
NcoI CCATGG 1 cut(s) 93
NdeII GATC 2 cut(s) 67, 520
NheI GCTAGC 2 cut(s) 313, 587
NlaIII CATG 6 cut(s) 97, 263, 274, 291, 359, 439
NlaIV GGNNCC 1 cut(s) 455
NspI RCATGY 1 cut(s) 439
PagI TCATGA 1 cut(s) 355
PciI ACATGT 1 cut(s) 435
PfeI GAWTC 2 cut(s) 83, 359
PkrI GCNGC 2 cut(s) 418, 502
PleI GAGTC 3 cut(s) 147, 162, 544
PpsI GAGTC 3 cut(s) 147, 162, 544
PscI ACATGT 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 235
RsaI GTAC 4 cut(s) 215, 389, 411, 515
RsaNI GTAC 4 cut(s) 214, 388, 410, 514
RseI CAYNNNNRTG 1 cut(s) 258
SatI GCNGC 2 cut(s) 417, 501
Sau3AI GATC 2 cut(s) 67, 520
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 3 cut(s) 148, 163, 545
SfaNI GCATC 2 cut(s) 270, 376
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 3 cut(s) 20, 28, 248
SsiI CCGC 5 cut(s) 200, 373, 416, 457, 495
SspMI CTAG 2 cut(s) 314, 588
StyI CCWWGG 3 cut(s) 93, 242, 449
TaiI ACGT 1 cut(s) 519
TaqI TCGA 1 cut(s) 562
TasI AATT 3 cut(s) 20, 28, 248
TatI WGTACW 2 cut(s) 213, 409
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 83, 359
TscAI CASTG 1 cut(s) 510
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 3 cut(s) 259, 321, 344
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XspI CTAG 2 cut(s) 314, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.