Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Forward (+)

2169746 .. 2178164

Gene Structure 8419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0027100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3405 bp

ATGTCCTCGCTGAGCAGAGAGCTTGTGTTTCTCATACTTCAGTTTCTGGATGAAGAGAAATTCAAAGAGAGTGTTCACAAGTTGGAGCAAGAGTCAGGGTTTTTCTTTAACATGAGGTACTTTGAAGATATGGTGACAAATGGGGAGTGGGAAGAGGTGGAGAAGTATTTGTCTGGTTTCACCAAGGTTGATGATAATAGATACTCTATGAAGATCTTTTTCGAGATACGCAAGCAGAAGTACCTTGAAGCTTTAGACAAGAGGGATCGAGCAAAAGCTGTGGACATTTTAGTCAAGGACTTGAAAGTGTTTGCAGCATTCAATGAAGAACTTTTTAAGGAAATTACACAGCTGTTGACTCTGGAGAACTTTAGGGATAATGAACAGTTATCTAAGTATGGTGATACAAAGTCTGCAAGGGGTATAATGCTTGCTGAACTGAAAAAGTTAATAGAGGCTAACCCATTGTTTCGTGATAAACTTCAATTTCCTACCCTGAAAAACTCAAGATTGCGGACTCTTATCAATCAGAGCTTAAACTGGCAGCACCAGCTTTGTAAGAATCCAAGGCCTAACCCTGACATAAAGACCCTATTTGTAGACCATAGCTGTGGACAACCGAATGGTGCCAGGGCTCCATCCCCTGTTACTAATCATTTAATGGGGGCAGTCCCAAAGACGGGGGGTTTCCCACCACTTGGTGCTCATGGACCCTTTCAGCCCACACCTGCTGCGCTTCCAACATCTCTTGCTGGATGGATGGCCAACTCATCACCTGTGCCTCATCCCTCTGCTTCCGCCGGGCCGATGGGTTTGGCTGCAGCTAACAATGCAGGAGCCCTCCTAAAGCGCCCTAGAACTCCTCCTACCAATAACCCAGCTATGGACTATCAAACAGCAGATTCTGAACATGTGATGAAGAGATCAAGACCTTTTGGAGTAACAGATGAGGCAAATAATCTGCCAGTAAATATTCTGCCAGTTGCTTATCCTAACCAGAGCCATGGTCAGAGTTCCTACTCTACTGATGACTTGCCCAGGAATGTAGTCATGACAATAAATCCAGGTTCAGCTGTCAAGAGTATGGATTTTCACCCAGTGCAACAGATTCTACTACTTGTTGGAACAAATATGGGTGATGTCATGATATATGAGTTACCAAGCCATGAAAGAATTGCTATTAGGAACTTCAAAGCCTGGGACCTTGCACAATGTTCAGTGGCTCTGCAGGCAACTTTGGCCAATGATTATACAGCATCGATCAACCGTGTAGTGTGGAGTCCTGATGGAACTCTTTTTGGTGTTGCATACTCTAAGCATCTAGTTCACATATACTCCTATCATGGTGGTGATGATTTAAGAAATCACCTAGAGATTGAGGCACATGCTGGCAGTGTTAATGATCTTGCTTTCTCATATCCAAACAAACAGCTCTGTGTTGTGACTTGTGGCGAGGATAGGGTTATCAAGGTGTGGGATGCTGTCACTGGGGCCAAGCAGCATACATTTGAGGGCCACGAAGCACCTGTATACTCGGTGTGCCCGCATCACAAGGAGAGTATTCAGTTTATTTTCTCGACAGCAACTGATGGAAAAATAAAGGCATGGCTGTATGATAATGTGGGCTCAAGAGTTGACTATGATGCGCCAGGACATTCGTCTACCACTATGGCATACAGTGCTGATGGATCAAGGCTATTCTCGTGTGGGACAAACAAAGAAGGGGATTCATACTTGGTGGAATGGAATGAAAGTGAAGGAGCTGTAAAGCGTACCTATCATGGTCTTGGGAAGCGGACTGTTGGGGTTGTGCAGTTTGATACCACCAAGAATCGATTCTTGGCTACTGGTGATGAGTTCATGGTTAAATTCTGGGACATGGACAATGTTAACCTTTTGACAAGTACAGATGCAGAAGGTGGACTGCCGGCTTCTCCTTCTATTAGATTTAACAAAGAAGGAATACTGTTAGCTGTCTCAACAAATGACAATGGTATTAAAATTCTAGCAAATTCAGATGGAATTAGGTTGCTGAGAACAGTGGAGAGTCGTACATTTGATGCTTCCAGAGCTGCTTCTGTGGCTGTTGTGAAGGCCCCAGCAATAGGAGCATTTGGATCGTCTAACATTACTGTTGGAACAGGCATTGGAGATCGAGCAGCTCCGGTGCCAGCCATGGTTGGACTGAACAATGACAGTCGAAGTTTGGCTGACGTGAAACCTAGAATTGTTGATGAGTCGATAGAGAAATCCAGGATATGGAAACTGACAGAAATCAATGAATCATCACAATGCCGGTCTCTGAGGCTTCCTGACAATTTAACGGCAATGAGGGTGTCTAGGTTAATTTACACAAATTCAGGACTTGCCATATTGGCATTATCATCTAATGCTGTGCACAAGCTCTGGAAATGGCAACGAAATGACAGAACCACGATAGGGAAGGCCACAGCTAGTATTGTACCACAACTGTGGCAACCTGCAAGTGGCATACTGATGACTAATGATATAAGTGATACAAACCCTGAAGATGCTGTTCCATGCTTTGCACTTTCAAAGAATGACTCTTACGTTATGTCAGCTTCAGGGGGGAAAATATCCCTGTTCAATATGATGACATTTAAGACGATGACAACATTCATGCCTCCACCCCCAGCTGCCACATATCTGGCATTTCATCCCCAAGACAATAATATCATTGCCATAGGCATGGAAGATTCTTCCATTCAGATTTACAATGTTCGGGTTGATGAGGTTAAAACTAAGCTAAAAGGTCATCAGAAAAGAATAACGGGCCTTGCCTTCTCACATGTTCTTAATGTGCTTGTATCCTCGGGAGCCGATTCTCAGTTGTGTGTTTGGAATACAGATGGATGGGAGAGGCAAGCTAGTAAATTCCTTCAGATTCCAAGTGGGCGAGCTGCTGCCCCTCTTGCAGATACTCGGGTTCAGTTCCACTTGGATCAGATACACTTGCTGGCAGTTCATGAAACACAGATAGCCATTTATGAAGCACCAAAATTAGAATGCCTCAAGCAGTTTGCCCCACGAGAGGCTAGTGGTCCAATCACACATGCTGCTTATTCCTGTGATAGCCAGTCAATTTATGTTAGCTTTGAAGATGGAAGTGTGGGGGTTCTTACTGCTACGACCCTCAGGTTGAGATGCCGAATATTACCAGCTGCTTATTTACCTCCAAATCCAAGCTTAAGAGTGTATCCTCTTGTTGTCGCGGCACATCCATCGGATCCCAATCAGTTTGCACTAGGTCTTACAGATGGTGGAGTGCATGTACTTGAGCCATTAGAATCAGAAGGAAAATGGGGCACCTCCCCGCCAACTGAAAATGGTGCTGGGCCTAGCACCGCTTCTGGAGCAGCAGGTTCGGATCAACCTCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1134

Amino Acids

124.54

Weight (kDa)

6.54

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	1.6e-12	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.2e-63	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1e-24	TOPLESS, zinc finger domain
Beta-prop_EML_2	PF23414	347 - 474	5.8e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	358 - 573	9.2e-11	WDR3 second beta-propeller domain
Beta-prop_RIG_2nd	PF23775	362 - 512	1.4e-07	RIG second beta-propeller
Beta-prop_WDR3_1st	PF25173	395 - 573	1.8e-16	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	414 - 572	2.3e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	417 - 630	1.9e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	419 - 677	4e-24	THOC3 beta-propeller domain
WDR55	PF24796	419 - 536	6.5e-07	WDR55
WD40_WDHD1_1st	PF24817	433 - 539	1.2e-10	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	457 - 664	2.1e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	477 - 668	4.5e-12	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	824 - 960	5.8e-13	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	840 - 1039	1.3e-16	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	840 - 962	2e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	841 - 1046	1.1e-15	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	841 - 957	1.1e-15	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	842 - 1056	4.7e-11	THOC3 beta-propeller domain
WDR55	PF24796	842 - 956	9.3e-07	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	843 - 955	8.6e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	844 - 1046	2.3e-16	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	847 - 963	1.7e-11	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	859 - 968	9.9e-10	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	860 - 956	7.1e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	864 - 958	8.6e-08	WDR90/POC16, second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	736
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc36I	ACCTGC	3 cut(s)	736, 2488, 3371
AccB1I	GGYRCC	3 cut(s)	626, 2167, 3326
AccB7I	CCANNNNNTGG	2 cut(s)	698, 2259
AccI	GTMKAC	3 cut(s)	600, 1530, 1661
AccII	CGCG	1 cut(s)	3233
AciI	CCGC	7 cut(s)	514, 798, 1544, 1795, 3233, 3335, 3366
AclWI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
AcoI	YGGCCR	2 cut(s)	762, 1239
AcsI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
AcuI	CTGAAG	4 cut(s)	23, 2547, 2568, 2885
AdeI	CACNNNGTG	2 cut(s)	701, 1099
AfaI	GTAC	7 cut(s)	119, 242, 1774, 1906, 2053, 2463, 3294
AfiI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
AflII	CTTAAG	1 cut(s)	3208
AflIII	ACRYGT	2 cut(s)	910, 2809
AjiI	CACGTC	1 cut(s)	2215
AjnI	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
AjuI	GAANNNNNNNTTGG	4 cut(s)	1820, 1823, 1852, 1855
AleI	CACNNNNGTG	1 cut(s)	2470
Alw21I	GWGCWC	2 cut(s)	706, 2400
Alw26I	GTCTC	2 cut(s)	1981, 2304
Alw44I	GTGCAC	1 cut(s)	2396
AlwI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
AlwNI	CAGNNNCTG	3 cut(s)	46, 905, 1586
Ama87I	CYCGRG	2 cut(s)	2833, 2943
ApaLI	GTGCAC	1 cut(s)	2396
ApoI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
Asp700I	GAANNNNTTC	1 cut(s)	1835
AspLEI	GCGC	3 cut(s)	736, 852, 1648
AspS9I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3062, 3356
AsuC2I	CCSGG	1 cut(s)	802
AsuHPI	GGTGA	9 cut(s)	145, 172, 413, 765, 1085, 1148, 1358, 1361, 1862
AvaI	CYCGRG	2 cut(s)	2833, 2943
AvaII	GGWCC	3 cut(s)	710, 1201, 3062
AxyI	CCTNAGG	1 cut(s)	3155
BaeGI	GKGCMC	3 cut(s)	1544, 2400, 3329
BaeI	ACNNNNGTAYC	2 cut(s)	2931, 2964
BalI	TGGCCA	2 cut(s)	764, 1241
BamHI	GGATCC	1 cut(s)	3247
BanI	GGYRCC	3 cut(s)	626, 2167, 3326
BanII	GRGCYC	3 cut(s)	637, 841, 1628
BauI	CACGAG	2 cut(s)	1702, 3048
Bbv12I	GWGCWC	2 cut(s)	706, 2400
BceAI	ACGGC	1 cut(s)	2340
BcgI	CGANNNNNNTGC	6 cut(s)	2100, 2134, 3225, 3259, 3366, 3400
BciT130I	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BciVI	GTATCC	2 cut(s)	2839, 3228
BcnI	CCSGG	1 cut(s)	802
BcoDI	GTCTC	2 cut(s)	1981, 2304
BfmI	CTRYAG	2 cut(s)	819, 1226
BfoI	RGCGCY	1 cut(s)	853
BfrI	CTTAAG	1 cut(s)	3208
BfuAI	ACCTGC	3 cut(s)	736, 2488, 3371
BfuI	GTATCC	2 cut(s)	2839, 3228
BglI	GCCNNNNNGGC	1 cut(s)	2375
BglII	AGATCT	1 cut(s)	213
BlpI	GCTNAGC	1 cut(s)	11
Bme1390I	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
Bme18I	GGWCC	3 cut(s)	710, 1201, 3062
BmeT110I	CYCGRG	2 cut(s)	2833, 2943
BmgBI	CACGTC	1 cut(s)	2215
BmgT120I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3062, 3356
BmrFI	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
BmrI	ACTGGG	2 cut(s)	1091, 1497
BmsI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
BmuI	ACTGGG	2 cut(s)	1091, 1497
BoxI	GACNNNNGTC	1 cut(s)	1657
BpmI	CTGGAG	2 cut(s)	383, 3393
Bpu1102I	GCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	4 cut(s)	490, 1612, 3017, 3317
BpuMI	CCSGG	1 cut(s)	802
Bsa29I	ATCGAT	2 cut(s)	1259, 1834
BsaBI	GATNNNNATC	1 cut(s)	3252
BsaI	GGTCTC	1 cut(s)	2304
BsaJI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BsaWI	WCCGGW	1 cut(s)	2164
BsaXI	ACNNNNNCTCC	6 cut(s)	77, 107, 1319, 1349, 3276, 3306
Bsc4I	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
Bse118I	RCCGGY	2 cut(s)	1927, 2295
Bse1I	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
Bse21I	CCTNAGG	1 cut(s)	3155
Bse3DI	GCAATG	2 cut(s)	2334, 2697
Bse8I	GATNNNNATC	1 cut(s)	3252
BseBI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BseCI	ATCGAT	2 cut(s)	1259, 1834
BseDI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BseGI	GGATG	9 cut(s)	55, 638, 761, 765, 784, 1483, 2677, 2879, 3238
BseJI	GATNNNNATC	1 cut(s)	3252
BseLI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BseMI	GCAATG	2 cut(s)	2334, 2697
BseMII	CTCAG	4 cut(s)	2024, 2294, 2861, 3169
BseNI	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
BseRI	GAGGAG	1 cut(s)	852
BseSI	GKGCMC	3 cut(s)	1544, 2400, 3329
BseYI	CCCAGC	4 cut(s)	877, 2098, 2653, 3353
BsgI	GTGCAG	1 cut(s)	1832
Bsh1236I	CGCG	1 cut(s)	3233
BshNI	GGYRCC	3 cut(s)	626, 2167, 3326
BshVI	ATCGAT	2 cut(s)	1259, 1834
BsiHKAI	GWGCWC	2 cut(s)	706, 2400
BsiHKCI	CYCGRG	2 cut(s)	2833, 2943
BsiSI	CCGG	4 cut(s)	801, 1928, 2165, 2296
BslFI	GGGAC	4 cut(s)	656, 1214, 1723, 1889
BslI	CCNNNNNNNGG	9 cut(s)	679, 680, 698, 883, 2105, 2259, 2439, 2486, 3052
BsmAI	GTCTC	2 cut(s)	1981, 2304
BsmFI	GGGAC	4 cut(s)	656, 1214, 1723, 1889
BsmI	GAATGC	2 cut(s)	317, 3032
Bso31I	GGTCTC	1 cut(s)	2304
BsoBI	CYCGRG	2 cut(s)	2833, 2943
Bsp1286I	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
Bsp1720I	GCTNAGC	1 cut(s)	11
Bsp19I	CCATGG	2 cut(s)	1003, 2175
BspACI	CCGC	7 cut(s)	514, 798, 1544, 1795, 3233, 3335, 3366
BspCNI	CTCAG	4 cut(s)	2025, 2295, 2860, 3168
BspDI	ATCGAT	2 cut(s)	1259, 1834
BspFNI	CGCG	1 cut(s)	3233
BspHI	TCATGA	3 cut(s)	1050, 1143, 2986
BspMAI	CTGCAG	2 cut(s)	823, 1230
BspMI	ACCTGC	3 cut(s)	736, 2488, 3371
BspPI	GGATC	7 cut(s)	273, 1696, 2125, 2970, 3242, 3255, 3396
BspT107I	GGYRCC	3 cut(s)	626, 2167, 3326
BspTI	CTTAAG	1 cut(s)	3208
BspTNI	GGTCTC	1 cut(s)	2304
BsrDI	GCAATG	2 cut(s)	2334, 2697
BsrFI	RCCGGY	2 cut(s)	1927, 2295
BsrI	ACTGG	7 cut(s)	545, 965, 980, 1097, 1492, 1852, 3097
BssAI	RCCGGY	2 cut(s)	1927, 2295
BssECI	CCNNGG	8 cut(s)	183, 566, 630, 1003, 1037, 1197, 2175, 2832
BssNAI	GTATAC	1 cut(s)	1531
BssSI	CACGAG	2 cut(s)	1702, 3048
BssT1I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
Bst1107I	GTATAC	1 cut(s)	1531
Bst2BI	CACGAG	2 cut(s)	1702, 3048
Bst2UI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
Bst4CI	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
Bst6I	CTCTTC	3 cut(s)	48, 147, 914
BstAFI	CTTAAG	1 cut(s)	3208
BstAPI	GCANNNNNTGC	1 cut(s)	1679
BstDEI	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
BstDSI	CCRYGG	2 cut(s)	1003, 2175
BstF5I	GGATG	9 cut(s)	55, 638, 761, 765, 784, 1483, 2677, 2879, 3238
BstFNI	CGCG	1 cut(s)	3233
BstH2I	RGCGCY	1 cut(s)	853
BstHHI	GCGC	3 cut(s)	736, 852, 1648
BstMAI	GTCTC	2 cut(s)	1981, 2304
BstNI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
BstNSI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
BstPAI	GACNNNNGTC	1 cut(s)	1657
BstSCI	CCNGG	7 cut(s)	629, 800, 1037, 1063, 1196, 1648, 2252
BstSFI	CTRYAG	2 cut(s)	819, 1226
BstSLI	GKGCMC	3 cut(s)	1544, 2400, 3329
BstUI	CGCG	1 cut(s)	3233
BstX2I	RGATCY	2 cut(s)	213, 3247
BstXI	CCANNNNNNTGG	5 cut(s)	611, 1004, 2472, 2668, 2710
BstYI	RGATCY	2 cut(s)	213, 3247
BstZ17I	GTATAC	1 cut(s)	1531
Bsu15I	ATCGAT	2 cut(s)	1259, 1834
Bsu36I	CCTNAGG	1 cut(s)	3155
BsuI	GTATCC	2 cut(s)	2839, 3228
BsuTUI	ATCGAT	2 cut(s)	1259, 1834
BtgI	CCRYGG	2 cut(s)	1003, 2175
BtrI	CACGTC	1 cut(s)	2215
BtsCI	GGATG	9 cut(s)	55, 638, 761, 765, 784, 1483, 2677, 2879, 3238
BtsI	GCAGTG	1 cut(s)	1399
BtsIMutI	CAGTG	6 cut(s)	1104, 1224, 1399, 1485, 1684, 2046
BveI	ACCTGC	3 cut(s)	736, 2488, 3371
CaiI	CAGNNNCTG	3 cut(s)	46, 905, 1586
CciI	TCATGA	3 cut(s)	1050, 1143, 2986
CfoI	GCGC	3 cut(s)	736, 852, 1648
Cfr10I	RCCGGY	2 cut(s)	1927, 2295
Cfr13I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3062, 3356
ClaI	ATCGAT	2 cut(s)	1259, 1834
Csp6I	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
CspCI	CAANNNNNGTGG	2 cut(s)	261, 296
CviQI	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
DdeI	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
DraIII	CACNNNGTG	2 cut(s)	701, 1099
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	2 cut(s)	762, 1239
Eam1104I	CTCTTC	3 cut(s)	48, 147, 914
EarI	CTCTTC	3 cut(s)	48, 147, 914
EciI	GGCGGA	1 cut(s)	787
Eco130I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
Eco147I	AGGCCT	1 cut(s)	571
Eco24I	GRGCYC	3 cut(s)	637, 841, 1628
Eco31I	GGTCTC	1 cut(s)	2304
Eco47I	GGWCC	3 cut(s)	710, 1201, 3062
Eco57I	CTGAAG	4 cut(s)	23, 2547, 2568, 2885
Eco81I	CCTNAGG	1 cut(s)	3155
Eco88I	CYCGRG	2 cut(s)	2833, 2943
EcoO109I	RGGNCCY	2 cut(s)	1201, 2095
EcoRII	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
EcoT14I	CCWWGG	4 cut(s)	183, 566, 1003, 2175
EcoT38I	GRGCYC	3 cut(s)	637, 841, 1628
ErhI	CCWWGG	4 cut(s)	183, 566, 1003, 2175
FaqI	GGGAC	4 cut(s)	656, 1214, 1723, 1889
FauI	CCCGC	2 cut(s)	1551, 3342
FblI	GTMKAC	3 cut(s)	600, 1530, 1661
FokI	GGATG	9 cut(s)	62, 625, 768, 771, 772, 1490, 2664, 2886, 3225
FriOI	GRGCYC	3 cut(s)	637, 841, 1628
GlaI	GCGC	3 cut(s)	735, 851, 1647
GsaI	CCCAGC	4 cut(s)	881, 2102, 2657, 3357
GsuI	CTGGAG	2 cut(s)	383, 3393
HaeII	RGCGCY	1 cut(s)	853
HapII	CCGG	4 cut(s)	801, 1928, 2165, 2296
HhaI	GCGC	3 cut(s)	736, 852, 1648
Hin6I	GCGC	3 cut(s)	734, 850, 1646
HinP1I	GCGC	3 cut(s)	734, 850, 1646
HincII	GTYRAC	3 cut(s)	357, 1636, 1891
HindII	GTYRAC	3 cut(s)	357, 1636, 1891
HindIII	AAGCTT	2 cut(s)	249, 3205
HpaI	GTTAAC	1 cut(s)	1891
HpaII	CCGG	4 cut(s)	801, 1928, 2165, 2296
HphI	GGTGA	9 cut(s)	145, 172, 413, 765, 1085, 1148, 1358, 1361, 1862
HpyCH4III	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
HpyCH4IV	ACGT	2 cut(s)	2214, 2571
HpyF3I	CTNAG	8 cut(s)	11, 393, 1314, 2033, 2303, 2763, 2847, 3155
HpySE526I	ACGT	2 cut(s)	2214, 2571
HspAI	GCGC	3 cut(s)	734, 850, 1646
KroI	GCCGGC	1 cut(s)	1927
KroNI	GCCGGC	1 cut(s)	1929
KspAI	GTTAAC	1 cut(s)	1891
LmnI	GCTCC	8 cut(s)	85, 640, 836, 1760, 2108, 2167, 2837, 3374
LweI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
MaeII	ACGT	2 cut(s)	2214, 2571
MaeIII	GTNAC	6 cut(s)	133, 646, 940, 1155, 1441, 1483
MflI	RGATCY	2 cut(s)	213, 3247
MhlI	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
MlsI	TGGCCA	2 cut(s)	764, 1241
MluNI	TGGCCA	2 cut(s)	764, 1241
MlyI	GAGTC	7 cut(s)	101, 352, 511, 1288, 2056, 2246, 2558
MmeI	TCCRAC	5 cut(s)	63, 764, 1102, 2116, 2161
Mox20I	TGGCCA	2 cut(s)	764, 1241
MroNI	GCCGGC	1 cut(s)	1927
MroXI	GAANNNNTTC	1 cut(s)	1835
MscI	TGGCCA	2 cut(s)	764, 1241
MslI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
Msp20I	TGGCCA	2 cut(s)	764, 1241
MspA1I	CMGCKG	4 cut(s)	352, 1073, 2657, 3182
MspCI	CTTAAG	1 cut(s)	3208
MspI	CCGG	4 cut(s)	801, 1928, 2165, 2296
MspR9I	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
Mva1269I	GAATGC	2 cut(s)	317, 3032
MvaI	CCWGG	6 cut(s)	631, 1039, 1065, 1198, 1650, 2254
MvnI	CGCG	1 cut(s)	3233
NaeI	GCCGGC	1 cut(s)	1929
NciI	CCSGG	1 cut(s)	802
NcoI	CCATGG	2 cut(s)	1003, 2175
NgoMIV	GCCGGC	1 cut(s)	1927
NmuCI	GTSAC	3 cut(s)	133, 1441, 1483
NspI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
OliI	CACNNNNGTG	1 cut(s)	2470
PagI	TCATGA	3 cut(s)	1050, 1143, 2986
PaqCI	CACCTGC	1 cut(s)	736
PceI	AGGCCT	1 cut(s)	571
PciI	ACATGT	2 cut(s)	910, 2809
PctI	GAATGC	2 cut(s)	317, 3032
PdiI	GCCGGC	1 cut(s)	1929
PdmI	GAANNNNTTC	1 cut(s)	1835
PflMI	CCANNNNNTGG	2 cut(s)	698, 2259
PfoI	TCCNGGA	1 cut(s)	2252
PleI	GAGTC	7 cut(s)	100, 352, 511, 1287, 2055, 2245, 2558
PpsI	GAGTC	7 cut(s)	100, 352, 511, 1287, 2055, 2245, 2558
PpuMI	RGGWCCY	1 cut(s)	1201
PscI	ACATGT	2 cut(s)	910, 2809
PshAI	GACNNNNGTC	1 cut(s)	1657
Psp5II	RGGWCCY	1 cut(s)	1201
Psp6I	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
PspFI	CCCAGC	4 cut(s)	877, 2098, 2653, 3353
PspGI	CCWGG	6 cut(s)	629, 1037, 1063, 1196, 1648, 2252
PspPI	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3062, 3356
PspPPI	RGGWCCY	1 cut(s)	1201
PsrI	GAACNNNNNNTAC	2 cut(s)	850, 882
PstI	CTGCAG	2 cut(s)	823, 1230
PstNI	CAGNNNCTG	3 cut(s)	46, 905, 1586
PsuI	RGATCY	2 cut(s)	213, 3247
PvuII	CAGCTG	4 cut(s)	352, 1073, 2657, 3182
RsaI	GTAC	7 cut(s)	119, 242, 1774, 1906, 2053, 2463, 3294
RsaNI	GTAC	7 cut(s)	118, 241, 1773, 1905, 2052, 2462, 3293
RseI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
Sau96I	GGNCC	9 cut(s)	710, 803, 1201, 1491, 1513, 2095, 2794, 3062, 3356
SchI	GAGTC	7 cut(s)	101, 352, 511, 1288, 2056, 2246, 2558
ScrFI	CCNGG	7 cut(s)	631, 802, 1039, 1065, 1198, 1650, 2254
SduI	GDGCHC	7 cut(s)	637, 706, 841, 1544, 1628, 2400, 3329
SfaNI	GCATC	9 cut(s)	1265, 1327, 1468, 1555, 1633, 1900, 2050, 2521, 3155
SfcI	CTRYAG	2 cut(s)	819, 1226
SinI	GGWCC	3 cut(s)	710, 1201, 3062
SmiMI	CAYNNNNRTG	6 cut(s)	609, 1347, 2290, 2470, 2495, 2708
SmlI	CTYRAG	5 cut(s)	505, 1627, 3032, 3208, 3296
SmoI	CTYRAG	5 cut(s)	505, 1627, 3032, 3208, 3296
SseBI	AGGCCT	1 cut(s)	571
SsiI	CCGC	7 cut(s)	514, 798, 1544, 1795, 3233, 3335, 3366
SspI	AATATT	2 cut(s)	973, 3174
StuI	AGGCCT	1 cut(s)	571
StyD4I	CCNGG	7 cut(s)	629, 800, 1037, 1063, 1196, 1648, 2252
StyI	CCWWGG	4 cut(s)	183, 566, 1003, 2175
TaaI	ACNGT	9 cut(s)	387, 1268, 1679, 1801, 1968, 2041, 2134, 2198, 2472
TaiI	ACGT	2 cut(s)	2217, 2574
TaqI	TCGA	8 cut(s)	222, 268, 1259, 1577, 1834, 2155, 2200, 2240
TatI	WGTACW	2 cut(s)	1904, 3292
TauI	GCSGC	1 cut(s)	3236
TscAI	CASTG	6 cut(s)	1104, 1224, 1399, 1492, 1684, 2046
TseFI	GTSAC	3 cut(s)	133, 1441, 1483
Tsp45I	GTSAC	3 cut(s)	133, 1441, 1483
TspRI	CASTG	6 cut(s)	1104, 1224, 1399, 1492, 1684, 2046
Van91I	CCANNNNNTGG	2 cut(s)	698, 2259
Vha464I	CTTAAG	1 cut(s)	3208
VneI	GTGCAC	1 cut(s)	2396
VpaK11BI	GGWCC	3 cut(s)	710, 1201, 3062
XapI	RAATTY	6 cut(s)	59, 1868, 2001, 2011, 2356, 2894
XceI	RCATGY	5 cut(s)	914, 1388, 2813, 3077, 3293
XmiI	GTMKAC	3 cut(s)	600, 1530, 1661
XmnI	GAANNNNTTC	1 cut(s)	1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.