Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1155 |
| AarI | CACCTGC | 1 cut(s) | 1423 |
| Acc36I | ACCTGC | 2 cut(s) | 1032, 1423 |
| AccB1I | GGYRCC | 2 cut(s) | 69, 2044 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1166 |
| AccI | GTMKAC | 1 cut(s) | 1984 |
| AccII | CGCG | 1 cut(s) | 1666 |
| AciI | CCGC | 4 cut(s) | 796, 1508, 1541, 2114 |
| AclWI | GGATC | 4 cut(s) | 47, 1530, 1704, 1948 |
| AcsI | RAATTY | 1 cut(s) | 1741 |
| AcuI | CTGAAG | 2 cut(s) | 1011, 1440 |
| AcyI | GRCGYC | 1 cut(s) | 70 |
| AfaI | GTAC | 3 cut(s) | 1014, 1329, 2139 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 79, 905, 1166, 1997, 1998, 2023, 2054 |
| AflII | CTTAAG | 1 cut(s) | 701 |
| AgeI | ACCGGT | 2 cut(s) | 1607, 1901 |
| AgsI | TTSAA | 9 cut(s) | 220, 305, 760, 824, 881, 967, 1040, 1554, 2105 |
| AjiI | CACGTC | 1 cut(s) | 2022 |
| AjnI | CCWGG | 3 cut(s) | 243, 1073, 1129 |
| AleI | CACNNNNGTG | 1 cut(s) | 1756 |
| Alw21I | GWGCWC | 4 cut(s) | 416, 1501, 1854, 2016 |
| Alw26I | GTCTC | 1 cut(s) | 2062 |
| Alw44I | GTGCAC | 2 cut(s) | 412, 1497 |
| AlwI | GGATC | 4 cut(s) | 47, 1530, 1704, 1948 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 182 |
| Ama87I | CYCGRG | 2 cut(s) | 482, 1996 |
| AoxI | GGCC | 4 cut(s) | 381, 627, 1110, 1251 |
| ApaLI | GTGCAC | 2 cut(s) | 412, 1497 |
| ApeKI | GCWGC | 8 cut(s) | 176, 179, 182, 211, 446, 977, 1825, 1927 |
| ApoI | RAATTY | 1 cut(s) | 1741 |
| AsiGI | ACCGGT | 2 cut(s) | 1607, 1901 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1082 |
| AspLEI | GCGC | 2 cut(s) | 72, 1666 |
| AspS9I | GGNCC | 4 cut(s) | 332, 627, 1110, 1252 |
| AsuC2I | CCSGG | 1 cut(s) | 904 |
| AsuHPI | GGTGA | 7 cut(s) | 176, 596, 919, 1490, 1591, 1846, 1962 |
| AvaI | CYCGRG | 2 cut(s) | 482, 1996 |
| AvaII | GGWCC | 1 cut(s) | 332 |
| BaeGI | GKGCMC | 3 cut(s) | 416, 1501, 2049 |
| BanI | GGYRCC | 2 cut(s) | 69, 2044 |
| BanII | GRGCYC | 3 cut(s) | 42, 1059, 1854 |
| BauI | CACGAG | 1 cut(s) | 377 |
| BbsI | GAAGAC | 3 cut(s) | 9, 102, 1615 |
| Bbv12I | GWGCWC | 4 cut(s) | 416, 1501, 1854, 2016 |
| BbvCI | CCTCAGC | 1 cut(s) | 1575 |
| BbvI | GCAGC | 8 cut(s) | 169, 188, 191, 223, 433, 964, 1837, 1939 |
| BccI | CCATC | 6 cut(s) | 155, 617, 725, 1216, 1498, 1996 |
| BceAI | ACGGC | 1 cut(s) | 1266 |
| BciT130I | CCWGG | 3 cut(s) | 245, 1075, 1131 |
| BclI | TGATCA | 1 cut(s) | 730 |
| BcnI | CCSGG | 1 cut(s) | 904 |
| BcoDI | GTCTC | 1 cut(s) | 2062 |
| BfaI | CTAG | 5 cut(s) | 1457, 1488, 1536, 1782, 1865 |
| BfmI | CTRYAG | 2 cut(s) | 604, 1881 |
| BfoI | RGCGCY | 1 cut(s) | 73 |
| BfrI | CTTAAG | 1 cut(s) | 701 |
| BfuAI | ACCTGC | 2 cut(s) | 1032, 1423 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1097 |
| BisI | GCNGC | 8 cut(s) | 177, 180, 183, 212, 447, 978, 1826, 1928 |
| BlpI | GCTNAGC | 1 cut(s) | 1570 |
| BlsI | GCNGC | 8 cut(s) | 178, 181, 184, 213, 448, 979, 1827, 1929 |
| Bme1390I | CCNGG | 4 cut(s) | 245, 904, 1075, 1131 |
| Bme18I | GGWCC | 1 cut(s) | 332 |
| BmeT110I | CYCGRG | 2 cut(s) | 482, 1996 |
| BmgBI | CACGTC | 1 cut(s) | 2022 |
| BmgT120I | GGNCC | 4 cut(s) | 332, 627, 1110, 1252 |
| BmiI | GGNNCC | 8 cut(s) | 39, 71, 628, 1072, 1111, 1254, 1878, 2046 |
| BmrFI | CCNGG | 4 cut(s) | 245, 904, 1075, 1131 |
| BmrI | ACTGGG | 1 cut(s) | 348 |
| BmsI | GCATC | 4 cut(s) | 163, 547, 1378, 1642 |
| BmuI | ACTGGG | 1 cut(s) | 348 |
| BpiI | GAAGAC | 3 cut(s) | 9, 102, 1615 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1841, 1873 |
| BpmI | CTGGAG | 2 cut(s) | 227, 2150 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1575 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1570 |
| BpuEI | CTTGAG | 2 cut(s) | 1050, 1841 |
| BpuMI | CCSGG | 1 cut(s) | 904 |
| BsaAI | YACGTR | 1 cut(s) | 749 |
| BsaBI | GATNNNNATC | 1 cut(s) | 710 |
| BsaHI | GRCGYC | 1 cut(s) | 70 |
| BsaJI | CCNNGG | 3 cut(s) | 903, 1129, 1130 |
| BsaWI | WCCGGW | 3 cut(s) | 1607, 1901, 2054 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1998, 2028 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 79, 905, 1166, 1997, 1998, 2023, 2054 |
| Bse118I | RCCGGY | 3 cut(s) | 1607, 1901, 2124 |
| Bse1I | ACTGG | 3 cut(s) | 343, 1171, 2145 |
| Bse3DI | GCAATG | 3 cut(s) | 567, 1701, 1706 |
| Bse8I | GATNNNNATC | 1 cut(s) | 710 |
| BseBI | CCWGG | 3 cut(s) | 245, 1075, 1131 |
| BseDI | CCNNGG | 3 cut(s) | 903, 1129, 1130 |
| BseGI | GGATG | 7 cut(s) | 628, 804, 862, 1227, 1987, 2007, 2149 |
| BseJI | GATNNNNATC | 1 cut(s) | 710 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 79, 905, 1166, 1997, 1998, 2023, 2054 |
| BseMI | GCAATG | 3 cut(s) | 567, 1701, 1706 |
| BseMII | CTCAG | 4 cut(s) | 504, 1464, 1566, 1584 |
| BseNI | ACTGG | 3 cut(s) | 343, 1171, 2145 |
| BseRI | GAGGAG | 1 cut(s) | 1807 |
| BseSI | GKGCMC | 3 cut(s) | 416, 1501, 2049 |
| BseXI | GCAGC | 8 cut(s) | 169, 188, 191, 223, 433, 964, 1837, 1939 |
| BseYI | CCCAGC | 1 cut(s) | 41 |
| BsgI | GTGCAG | 2 cut(s) | 230, 858 |
| Bsh1236I | CGCG | 1 cut(s) | 1666 |
| BshFI | GGCC | 4 cut(s) | 383, 629, 1112, 1253 |
| BshNI | GGYRCC | 2 cut(s) | 69, 2044 |
| BshTI | ACCGGT | 2 cut(s) | 1607, 1901 |
| BsiHKAI | GWGCWC | 4 cut(s) | 416, 1501, 1854, 2016 |
| BsiHKCI | CYCGRG | 2 cut(s) | 482, 1996 |
| BsiSI | CCGG | 8 cut(s) | 73, 384, 903, 1608, 1902, 2055, 2090, 2125 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 79, 905, 1166, 1997, 1998, 2023, 2054 |
| BsmAI | GTCTC | 1 cut(s) | 2062 |
| BsnI | GGCC | 4 cut(s) | 383, 629, 1112, 1253 |
| BsoBI | CYCGRG | 2 cut(s) | 482, 1996 |
| Bsp1286I | GDGCHC | 7 cut(s) | 42, 416, 1059, 1501, 1854, 2016, 2049 |
| Bsp143I | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1570 |
| BspACI | CCGC | 4 cut(s) | 796, 1508, 1541, 2114 |
| BspANI | GGCC | 4 cut(s) | 383, 629, 1112, 1253 |
| BspCNI | CTCAG | 4 cut(s) | 505, 1465, 1567, 1583 |
| BspFNI | CGCG | 1 cut(s) | 1666 |
| BspHI | TCATGA | 1 cut(s) | 2035 |
| BspLI | GGNNCC | 8 cut(s) | 39, 71, 628, 1072, 1111, 1254, 1878, 2046 |
| BspMI | ACCTGC | 2 cut(s) | 1032, 1423 |
| BspPI | GGATC | 4 cut(s) | 47, 1530, 1704, 1948 |
| BspQI | GCTCTTC | 1 cut(s) | 987 |
| BspT107I | GGYRCC | 2 cut(s) | 69, 2044 |
| BspTI | CTTAAG | 1 cut(s) | 701 |
| BsrDI | GCAATG | 3 cut(s) | 567, 1701, 1706 |
| BsrFI | RCCGGY | 3 cut(s) | 1607, 1901, 2124 |
| BsrI | ACTGG | 3 cut(s) | 343, 1171, 2145 |
| BssAI | RCCGGY | 3 cut(s) | 1607, 1901, 2124 |
| BssECI | CCNNGG | 3 cut(s) | 903, 1129, 1130 |
| BssMI | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| BssNAI | GTATAC | 1 cut(s) | 1985 |
| BssNI | GRCGYC | 1 cut(s) | 70 |
| BssSI | CACGAG | 1 cut(s) | 377 |
| Bst1107I | GTATAC | 1 cut(s) | 1985 |
| Bst2BI | CACGAG | 1 cut(s) | 377 |
| Bst2UI | CCWGG | 3 cut(s) | 245, 1075, 1131 |
| Bst4CI | ACNGT | 6 cut(s) | 538, 1096, 1318, 1392, 1755, 1954 |
| Bst6I | CTCTTC | 3 cut(s) | 99, 933, 987 |
| BstACI | GRCGYC | 1 cut(s) | 70 |
| BstAFI | CTTAAG | 1 cut(s) | 701 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 182 |
| BstBAI | YACGTR | 1 cut(s) | 749 |
| BstC8I | GCNNGC | 2 cut(s) | 982, 1029 |
| BstDEI | CTNAG | 6 cut(s) | 513, 1358, 1473, 1570, 1575, 1712 |
| BstF5I | GGATG | 7 cut(s) | 628, 804, 862, 1227, 1987, 2007, 2149 |
| BstFNI | CGCG | 1 cut(s) | 1666 |
| BstH2I | RGCGCY | 1 cut(s) | 73 |
| BstHHI | GCGC | 2 cut(s) | 72, 1666 |
| BstKTI | GATC | 9 cut(s) | 55, 133, 733, 945, 1285, 1525, 1699, 1943, 2085 |
| BstMAI | GTCTC | 1 cut(s) | 2062 |
| BstMBI | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 182, 443, 986, 1033, 1097, 1505, 1547, 1661, 1825 |
| BstNI | CCWGG | 3 cut(s) | 245, 1075, 1131 |
| BstSCI | CCNGG | 4 cut(s) | 243, 902, 1073, 1129 |
| BstSFI | CTRYAG | 2 cut(s) | 604, 1881 |
| BstSLI | GKGCMC | 3 cut(s) | 416, 1501, 2049 |
| BstUI | CGCG | 1 cut(s) | 1666 |
| BstV1I | GCAGC | 8 cut(s) | 169, 188, 191, 223, 433, 964, 1837, 1939 |
| BstV2I | GAAGAC | 3 cut(s) | 9, 102, 1615 |
| BstX2I | RGATCY | 2 cut(s) | 52, 1940 |
| BstYI | RGATCY | 2 cut(s) | 52, 1940 |
| BstZ17I | GTATAC | 1 cut(s) | 1985 |
| BsuRI | GGCC | 4 cut(s) | 383, 629, 1112, 1253 |
| BtrI | CACGTC | 1 cut(s) | 2022 |
| BtsCI | GGATG | 7 cut(s) | 628, 804, 862, 1227, 1987, 2007, 2149 |
| BtsI | GCAGTG | 1 cut(s) | 870 |
| BtsIMutI | CAGTG | 4 cut(s) | 870, 1101, 1323, 1760 |
| BveI | ACCTGC | 2 cut(s) | 1032, 1423 |
| Cac8I | GCNNGC | 2 cut(s) | 982, 1029 |
| CaiI | CAGNNNCTG | 1 cut(s) | 182 |
| CciI | TCATGA | 1 cut(s) | 2035 |
| CfoI | GCGC | 2 cut(s) | 72, 1666 |
| Cfr10I | RCCGGY | 3 cut(s) | 1607, 1901, 2124 |
| Cfr13I | GGNCC | 4 cut(s) | 332, 627, 1110, 1252 |
| Csp6I | GTAC | 3 cut(s) | 1013, 1328, 2138 |
| CspAI | ACCGGT | 2 cut(s) | 1607, 1901 |
| CviQI | GTAC | 3 cut(s) | 1013, 1328, 2138 |
| DdeI | CTNAG | 6 cut(s) | 513, 1358, 1473, 1570, 1575, 1712 |
| DinI | GGCGCC | 1 cut(s) | 71 |
| DpnI | GATC | 9 cut(s) | 54, 132, 732, 944, 1284, 1524, 1698, 1942, 2084 |
| DpnII | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| Eam1104I | CTCTTC | 3 cut(s) | 99, 933, 987 |
| EarI | CTCTTC | 3 cut(s) | 99, 933, 987 |
| EciI | GGCGGA | 2 cut(s) | 811, 1523 |
| Ecl136II | GAGCTC | 1 cut(s) | 1852 |
| Eco24I | GRGCYC | 3 cut(s) | 42, 1059, 1854 |
| Eco32I | GATATC | 1 cut(s) | 655 |
| Eco47I | GGWCC | 1 cut(s) | 332 |
| Eco53kI | GAGCTC | 1 cut(s) | 1852 |
| Eco57I | CTGAAG | 2 cut(s) | 1011, 1440 |
| Eco88I | CYCGRG | 2 cut(s) | 482, 1996 |
| EcoICRI | GAGCTC | 1 cut(s) | 1852 |
| EcoRII | CCWGG | 3 cut(s) | 243, 1073, 1129 |
| EcoRV | GATATC | 1 cut(s) | 655 |
| EcoT22I | ATGCAT | 1 cut(s) | 1729 |
| EcoT38I | GRGCYC | 3 cut(s) | 42, 1059, 1854 |
| EgeI | GGCGCC | 1 cut(s) | 71 |
| EheI | GGCGCC | 1 cut(s) | 71 |
| FauNDI | CATATG | 1 cut(s) | 1527 |
| FbaI | TGATCA | 1 cut(s) | 730 |
| FblI | GTMKAC | 1 cut(s) | 1984 |
| Fnu4HI | GCNGC | 8 cut(s) | 177, 180, 183, 212, 447, 978, 1826, 1928 |
| FokI | GGATG | 6 cut(s) | 635, 811, 869, 1234, 1974, 2014 |
| FriOI | GRGCYC | 3 cut(s) | 42, 1059, 1854 |
| Fsp4HI | GCNGC | 8 cut(s) | 177, 180, 183, 212, 447, 978, 1826, 1928 |
| FspBI | CTAG | 5 cut(s) | 1457, 1488, 1536, 1782, 1865 |
| GlaI | GCGC | 2 cut(s) | 71, 1665 |
| GluI | GCNGC | 8 cut(s) | 177, 180, 183, 212, 447, 978, 1826, 1928 |
| GsaI | CCCAGC | 1 cut(s) | 45 |
| GsuI | CTGGAG | 2 cut(s) | 227, 2150 |
| HaeII | RGCGCY | 1 cut(s) | 73 |
| HaeIII | GGCC | 4 cut(s) | 383, 629, 1112, 1253 |
| HapII | CCGG | 8 cut(s) | 73, 384, 903, 1608, 1902, 2055, 2090, 2125 |
| HhaI | GCGC | 2 cut(s) | 72, 1666 |
| Hin1I | GRCGYC | 1 cut(s) | 70 |
| Hin6I | GCGC | 2 cut(s) | 70, 1664 |
| HinP1I | GCGC | 2 cut(s) | 70, 1664 |
| HindIII | AAGCTT | 1 cut(s) | 1817 |
| HinfI | GANTC | 4 cut(s) | 88, 310, 1616, 1967 |
| HpaII | CCGG | 8 cut(s) | 73, 384, 903, 1608, 1902, 2055, 2090, 2125 |
| HphI | GGTGA | 7 cut(s) | 176, 596, 919, 1490, 1591, 1846, 1962 |
| Hpy166II | GTNNAC | 6 cut(s) | 284, 414, 587, 1499, 1720, 1985 |
| Hpy188I | TCNGA | 6 cut(s) | 37, 52, 226, 514, 529, 722 |
| Hpy188III | TCNNGA | 8 cut(s) | 56, 92, 281, 453, 1763, 1811, 2017, 2036 |
| Hpy8I | GTNNAC | 6 cut(s) | 284, 414, 587, 1499, 1720, 1985 |
| Hpy99I | CGWCG | 1 cut(s) | 2023 |
| HpyAV | CCTTC | 4 cut(s) | 519, 1072, 1831, 1882 |
| HpyCH4III | ACNGT | 6 cut(s) | 538, 1096, 1318, 1392, 1755, 1954 |
| HpyCH4IV | ACGT | 2 cut(s) | 748, 2021 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 182, 443, 986, 1033, 1097, 1505, 1547, 1661, 1825 |
| HpyF3I | CTNAG | 6 cut(s) | 513, 1358, 1473, 1570, 1575, 1712 |
| HpySE526I | ACGT | 2 cut(s) | 748, 2021 |
| Hsp92I | GRCGYC | 1 cut(s) | 70 |
| HspAI | GCGC | 2 cut(s) | 70, 1664 |
| KasI | GGCGCC | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 1 cut(s) | 730 |
| Kzo9I | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| LguI | GCTCTTC | 1 cut(s) | 987 |
| LmnI | GCTCC | 8 cut(s) | 37, 226, 246, 772, 1070, 1876, 2019, 2116 |
| Lsp1109I | GCAGC | 8 cut(s) | 169, 188, 191, 223, 433, 964, 1837, 1939 |
| LweI | GCATC | 4 cut(s) | 163, 547, 1378, 1642 |
| MaeI | CTAG | 5 cut(s) | 1457, 1488, 1536, 1782, 1865 |
| MaeII | ACGT | 2 cut(s) | 748, 2021 |
| MaeIII | GTNAC | 4 cut(s) | 744, 809, 1760, 2074 |
| MalI | GATC | 9 cut(s) | 54, 132, 732, 944, 1284, 1524, 1698, 1942, 2084 |
| MboI | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| MfeI | CAATTG | 3 cut(s) | 287, 962, 1554 |
| MflI | RGATCY | 2 cut(s) | 52, 1940 |
| MhlI | GDGCHC | 7 cut(s) | 42, 416, 1059, 1501, 1854, 2016, 2049 |
| MluCI | AATT | 9 cut(s) | 215, 220, 287, 819, 962, 1288, 1381, 1554, 1741 |
| Mly113I | GGCGCC | 1 cut(s) | 70 |
| MlyI | GAGTC | 1 cut(s) | 97 |
| MmeI | TCCRAC | 1 cut(s) | 359 |
| Mph1103I | ATGCAT | 1 cut(s) | 1729 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1082 |
| MseI | TTAA | 6 cut(s) | 138, 671, 702, 1178, 1380, 2152 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1650, 1756 |
| MspA1I | CMGCKG | 2 cut(s) | 182, 1574 |
| MspCI | CTTAAG | 1 cut(s) | 701 |
| MspI | CCGG | 8 cut(s) | 73, 384, 903, 1608, 1902, 2055, 2090, 2125 |
| MspR9I | CCNGG | 4 cut(s) | 245, 904, 1075, 1131 |
| MunI | CAATTG | 3 cut(s) | 287, 962, 1554 |
| MvaI | CCWGG | 3 cut(s) | 245, 1075, 1131 |
| MvnI | CGCG | 1 cut(s) | 1666 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 182, 443, 986, 1033, 1097, 1505, 1547, 1661, 1825 |
| NarI | GGCGCC | 1 cut(s) | 70 |
| NciI | CCSGG | 1 cut(s) | 904 |
| NdeI | CATATG | 1 cut(s) | 1527 |
| NdeII | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| NlaIV | GGNNCC | 8 cut(s) | 39, 71, 628, 1072, 1111, 1254, 1878, 2046 |
| NmeAIII | GCCGAG | 1 cut(s) | 1057 |
| NmuCI | GTSAC | 2 cut(s) | 809, 1760 |
| NsiI | ATGCAT | 1 cut(s) | 1729 |
| OliI | CACNNNNGTG | 1 cut(s) | 1756 |
| PagI | TCATGA | 1 cut(s) | 2035 |
| PaqCI | CACCTGC | 1 cut(s) | 1423 |
| PasI | CCCWGGG | 1 cut(s) | 1130 |
| PciSI | GCTCTTC | 1 cut(s) | 987 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1082 |
| PfeI | GAWTC | 3 cut(s) | 310, 1616, 1967 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1166 |
| PfoI | TCCNGGA | 1 cut(s) | 243 |
| PinAI | ACCGGT | 2 cut(s) | 1607, 1901 |
| PkrI | GCNGC | 8 cut(s) | 178, 181, 184, 213, 448, 979, 1827, 1929 |
| PleI | GAGTC | 1 cut(s) | 96 |
| PluTI | GGCGCC | 1 cut(s) | 73 |
| PpsI | GAGTC | 1 cut(s) | 96 |
| Ppu21I | YACGTR | 1 cut(s) | 749 |
| PsiI | TTATAA | 1 cut(s) | 1155 |
| Psp124BI | GAGCTC | 1 cut(s) | 1854 |
| Psp6I | CCWGG | 3 cut(s) | 243, 1073, 1129 |
| PspFI | CCCAGC | 1 cut(s) | 41 |
| PspGI | CCWGG | 3 cut(s) | 243, 1073, 1129 |
| PspN4I | GGNNCC | 8 cut(s) | 39, 71, 628, 1072, 1111, 1254, 1878, 2046 |
| PspPI | GGNCC | 4 cut(s) | 332, 627, 1110, 1252 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 136, 168 |
| PstNI | CAGNNNCTG | 1 cut(s) | 182 |
| PsuI | RGATCY | 2 cut(s) | 52, 1940 |
| PvuII | CAGCTG | 2 cut(s) | 182, 1574 |
| RsaI | GTAC | 3 cut(s) | 1014, 1329, 2139 |
| RsaNI | GTAC | 3 cut(s) | 1013, 1328, 2138 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1650, 1756 |
| SacI | GAGCTC | 1 cut(s) | 1854 |
| SapI | GCTCTTC | 1 cut(s) | 987 |
| SaqAI | TTAA | 6 cut(s) | 138, 671, 702, 1178, 1380, 2152 |
| SatI | GCNGC | 8 cut(s) | 177, 180, 183, 212, 447, 978, 1826, 1928 |
| Sau3AI | GATC | 9 cut(s) | 52, 130, 730, 942, 1282, 1522, 1696, 1940, 2082 |
| Sau96I | GGNCC | 4 cut(s) | 332, 627, 1110, 1252 |
| SchI | GAGTC | 1 cut(s) | 97 |
| ScrFI | CCNGG | 4 cut(s) | 245, 904, 1075, 1131 |
| SduI | GDGCHC | 7 cut(s) | 42, 416, 1059, 1501, 1854, 2016, 2049 |
| SfaNI | GCATC | 4 cut(s) | 163, 547, 1378, 1642 |
| SfcI | CTRYAG | 2 cut(s) | 604, 1881 |
| SfoI | GGCGCC | 1 cut(s) | 71 |
| SinI | GGWCC | 1 cut(s) | 332 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1650, 1756 |
| SmlI | CTYRAG | 3 cut(s) | 701, 1065, 1820 |
| SmoI | CTYRAG | 3 cut(s) | 701, 1065, 1820 |
| Sse9I | AATT | 9 cut(s) | 215, 220, 287, 819, 962, 1288, 1381, 1554, 1741 |
| SsiI | CCGC | 4 cut(s) | 796, 1508, 1541, 2114 |
| SspDI | GGCGCC | 1 cut(s) | 69 |
| SspMI | CTAG | 5 cut(s) | 1457, 1488, 1536, 1782, 1865 |
| SstI | GAGCTC | 1 cut(s) | 1854 |
| StyD4I | CCNGG | 4 cut(s) | 243, 902, 1073, 1129 |
| TaaI | ACNGT | 6 cut(s) | 538, 1096, 1318, 1392, 1755, 1954 |
| TaiI | ACGT | 2 cut(s) | 751, 2024 |
| TaqI | TCGA | 4 cut(s) | 294, 685, 945, 1630 |
| TasI | AATT | 9 cut(s) | 215, 220, 287, 819, 962, 1288, 1381, 1554, 1741 |
| TatI | WGTACW | 2 cut(s) | 1327, 2137 |
| TfiI | GAWTC | 3 cut(s) | 310, 1616, 1967 |
| Tru1I | TTAA | 6 cut(s) | 138, 671, 702, 1178, 1380, 2152 |
| Tru9I | TTAA | 6 cut(s) | 138, 671, 702, 1178, 1380, 2152 |
| TscAI | CASTG | 4 cut(s) | 877, 1101, 1323, 1760 |
| TseFI | GTSAC | 2 cut(s) | 809, 1760 |
| TseI | GCWGC | 8 cut(s) | 176, 179, 182, 211, 446, 977, 1825, 1927 |
| Tsp45I | GTSAC | 2 cut(s) | 809, 1760 |
| TspDTI | ATGAA | 8 cut(s) | 117, 216, 588, 845, 951, 1032, 1667, 1949 |
| TspGWI | ACGGA | 1 cut(s) | 1970 |
| TspRI | CASTG | 4 cut(s) | 877, 1101, 1323, 1760 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1166 |
| Vha464I | CTTAAG | 1 cut(s) | 701 |
| VneI | GTGCAC | 2 cut(s) | 412, 1497 |
| VpaK11BI | GGWCC | 1 cut(s) | 332 |
| XapI | RAATTY | 1 cut(s) | 1741 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1329, 1999 |
| XmiI | GTMKAC | 1 cut(s) | 1984 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1082 |
| XspI | CTAG | 5 cut(s) | 1457, 1488, 1536, 1782, 1865 |
| Zsp2I | ATGCAT | 1 cut(s) | 1729 |