Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1275 |
| AarI | CACCTGC | 1 cut(s) | 1543 |
| Acc36I | ACCTGC | 2 cut(s) | 1152, 1543 |
| AccB1I | GGYRCC | 2 cut(s) | 189, 2164 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1286 |
| AccI | GTMKAC | 1 cut(s) | 2104 |
| AccII | CGCG | 1 cut(s) | 1786 |
| AciI | CCGC | 5 cut(s) | 53, 916, 1628, 1661, 2234 |
| AclI | AACGTT | 1 cut(s) | 11 |
| AclWI | GGATC | 4 cut(s) | 167, 1650, 1824, 2068 |
| AcsI | RAATTY | 1 cut(s) | 1861 |
| AcuI | CTGAAG | 2 cut(s) | 1131, 1560 |
| AcyI | GRCGYC | 1 cut(s) | 190 |
| AfaI | GTAC | 4 cut(s) | 69, 1134, 1449, 2259 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 90, 199, 1025, 1286, 2117, 2118, 2143, 2174 |
| AflII | CTTAAG | 1 cut(s) | 821 |
| AgeI | ACCGGT | 2 cut(s) | 1727, 2021 |
| AjiI | CACGTC | 1 cut(s) | 2142 |
| AjnI | CCWGG | 3 cut(s) | 363, 1193, 1249 |
| AleI | CACNNNNGTG | 1 cut(s) | 1876 |
| Alw21I | GWGCWC | 4 cut(s) | 536, 1621, 1974, 2136 |
| Alw26I | GTCTC | 1 cut(s) | 2182 |
| Alw44I | GTGCAC | 2 cut(s) | 532, 1617 |
| AlwI | GGATC | 4 cut(s) | 167, 1650, 1824, 2068 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 302 |
| Ama87I | CYCGRG | 2 cut(s) | 602, 2116 |
| AoxI | GGCC | 4 cut(s) | 501, 747, 1230, 1371 |
| ApaLI | GTGCAC | 2 cut(s) | 532, 1617 |
| ApeKI | GCWGC | 8 cut(s) | 296, 299, 302, 331, 566, 1097, 1945, 2047 |
| ApoI | RAATTY | 1 cut(s) | 1861 |
| AsiGI | ACCGGT | 2 cut(s) | 1727, 2021 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1202 |
| AspA2I | CCTAGG | 1 cut(s) | 59 |
| AspLEI | GCGC | 2 cut(s) | 192, 1786 |
| AspS9I | GGNCC | 4 cut(s) | 452, 747, 1230, 1372 |
| AsuC2I | CCSGG | 1 cut(s) | 1024 |
| AsuHPI | GGTGA | 7 cut(s) | 296, 716, 1039, 1610, 1711, 1966, 2082 |
| AvaI | CYCGRG | 2 cut(s) | 602, 2116 |
| AvaII | GGWCC | 1 cut(s) | 452 |
| AvrII | CCTAGG | 1 cut(s) | 59 |
| BaeGI | GKGCMC | 3 cut(s) | 536, 1621, 2169 |
| BanI | GGYRCC | 2 cut(s) | 189, 2164 |
| BanII | GRGCYC | 3 cut(s) | 162, 1179, 1974 |
| BauI | CACGAG | 1 cut(s) | 497 |
| BbsI | GAAGAC | 3 cut(s) | 129, 222, 1735 |
| Bbv12I | GWGCWC | 4 cut(s) | 536, 1621, 1974, 2136 |
| BbvCI | CCTCAGC | 1 cut(s) | 1695 |
| BbvI | GCAGC | 8 cut(s) | 289, 308, 311, 343, 553, 1084, 1957, 2059 |
| BccI | CCATC | 6 cut(s) | 275, 737, 845, 1336, 1618, 2116 |
| BceAI | ACGGC | 1 cut(s) | 1386 |
| BciT130I | CCWGG | 3 cut(s) | 365, 1195, 1251 |
| BclI | TGATCA | 1 cut(s) | 850 |
| BcnI | CCSGG | 1 cut(s) | 1024 |
| BcoDI | GTCTC | 1 cut(s) | 2182 |
| BfaI | CTAG | 6 cut(s) | 60, 1577, 1608, 1656, 1902, 1985 |
| BfmI | CTRYAG | 2 cut(s) | 724, 2001 |
| BfoI | RGCGCY | 1 cut(s) | 193 |
| BfrI | CTTAAG | 1 cut(s) | 821 |
| BfuAI | ACCTGC | 2 cut(s) | 1152, 1543 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1217 |
| BisI | GCNGC | 8 cut(s) | 297, 300, 303, 332, 567, 1098, 1946, 2048 |
| BlnI | CCTAGG | 1 cut(s) | 59 |
| BlpI | GCTNAGC | 1 cut(s) | 1690 |
| BlsI | GCNGC | 8 cut(s) | 298, 301, 304, 333, 568, 1099, 1947, 2049 |
| Bme1390I | CCNGG | 4 cut(s) | 365, 1024, 1195, 1251 |
| Bme18I | GGWCC | 1 cut(s) | 452 |
| BmeT110I | CYCGRG | 2 cut(s) | 602, 2116 |
| BmgBI | CACGTC | 1 cut(s) | 2142 |
| BmgT120I | GGNCC | 4 cut(s) | 452, 747, 1230, 1372 |
| BmiI | GGNNCC | 8 cut(s) | 159, 191, 748, 1192, 1231, 1374, 1998, 2166 |
| BmrFI | CCNGG | 4 cut(s) | 365, 1024, 1195, 1251 |
| BmrI | ACTGGG | 1 cut(s) | 468 |
| BmsI | GCATC | 4 cut(s) | 283, 667, 1498, 1762 |
| BmuI | ACTGGG | 1 cut(s) | 468 |
| BpiI | GAAGAC | 3 cut(s) | 129, 222, 1735 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1961, 1993 |
| BpmI | CTGGAG | 2 cut(s) | 347, 2270 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1695 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1690 |
| BpuEI | CTTGAG | 3 cut(s) | 73, 1170, 1961 |
| BpuMI | CCSGG | 1 cut(s) | 1024 |
| BsaAI | YACGTR | 1 cut(s) | 869 |
| BsaBI | GATNNNNATC | 1 cut(s) | 830 |
| BsaHI | GRCGYC | 1 cut(s) | 190 |
| BsaJI | CCNNGG | 5 cut(s) | 33, 59, 1023, 1249, 1250 |
| BsaWI | WCCGGW | 3 cut(s) | 1727, 2021, 2174 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2118, 2148 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 90, 199, 1025, 1286, 2117, 2118, 2143, 2174 |
| Bse118I | RCCGGY | 3 cut(s) | 1727, 2021, 2244 |
| Bse1I | ACTGG | 3 cut(s) | 463, 1291, 2265 |
| Bse3DI | GCAATG | 3 cut(s) | 687, 1821, 1826 |
| Bse8I | GATNNNNATC | 1 cut(s) | 830 |
| BseBI | CCWGG | 3 cut(s) | 365, 1195, 1251 |
| BseDI | CCNNGG | 5 cut(s) | 33, 59, 1023, 1249, 1250 |
| BseGI | GGATG | 7 cut(s) | 748, 924, 982, 1347, 2107, 2127, 2269 |
| BseJI | GATNNNNATC | 1 cut(s) | 830 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 90, 199, 1025, 1286, 2117, 2118, 2143, 2174 |
| BseMI | GCAATG | 3 cut(s) | 687, 1821, 1826 |
| BseMII | CTCAG | 4 cut(s) | 624, 1584, 1686, 1704 |
| BseNI | ACTGG | 3 cut(s) | 463, 1291, 2265 |
| BseRI | GAGGAG | 1 cut(s) | 1927 |
| BseSI | GKGCMC | 3 cut(s) | 536, 1621, 2169 |
| BseXI | GCAGC | 8 cut(s) | 289, 308, 311, 343, 553, 1084, 1957, 2059 |
| BseYI | CCCAGC | 1 cut(s) | 161 |
| BsgI | GTGCAG | 2 cut(s) | 350, 978 |
| Bsh1236I | CGCG | 1 cut(s) | 1786 |
| BshFI | GGCC | 4 cut(s) | 503, 749, 1232, 1373 |
| BshNI | GGYRCC | 2 cut(s) | 189, 2164 |
| BshTI | ACCGGT | 2 cut(s) | 1727, 2021 |
| BsiHKAI | GWGCWC | 4 cut(s) | 536, 1621, 1974, 2136 |
| BsiHKCI | CYCGRG | 2 cut(s) | 602, 2116 |
| BsiSI | CCGG | 8 cut(s) | 193, 504, 1023, 1728, 2022, 2175, 2210, 2245 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 90, 199, 1025, 1286, 2117, 2118, 2143, 2174 |
| BsmAI | GTCTC | 1 cut(s) | 2182 |
| BsnI | GGCC | 4 cut(s) | 503, 749, 1232, 1373 |
| BsoBI | CYCGRG | 2 cut(s) | 602, 2116 |
| Bsp1286I | GDGCHC | 7 cut(s) | 162, 536, 1179, 1621, 1974, 2136, 2169 |
| Bsp143I | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1690 |
| BspACI | CCGC | 5 cut(s) | 53, 916, 1628, 1661, 2234 |
| BspANI | GGCC | 4 cut(s) | 503, 749, 1232, 1373 |
| BspCNI | CTCAG | 4 cut(s) | 625, 1585, 1687, 1703 |
| BspFNI | CGCG | 1 cut(s) | 1786 |
| BspHI | TCATGA | 1 cut(s) | 2155 |
| BspLI | GGNNCC | 8 cut(s) | 159, 191, 748, 1192, 1231, 1374, 1998, 2166 |
| BspMI | ACCTGC | 2 cut(s) | 1152, 1543 |
| BspPI | GGATC | 4 cut(s) | 167, 1650, 1824, 2068 |
| BspQI | GCTCTTC | 1 cut(s) | 1107 |
| BspT107I | GGYRCC | 2 cut(s) | 189, 2164 |
| BspTI | CTTAAG | 1 cut(s) | 821 |
| BsrDI | GCAATG | 3 cut(s) | 687, 1821, 1826 |
| BsrFI | RCCGGY | 3 cut(s) | 1727, 2021, 2244 |
| BsrI | ACTGG | 3 cut(s) | 463, 1291, 2265 |
| BssAI | RCCGGY | 3 cut(s) | 1727, 2021, 2244 |
| BssECI | CCNNGG | 5 cut(s) | 33, 59, 1023, 1249, 1250 |
| BssMI | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| BssNAI | GTATAC | 1 cut(s) | 2105 |
| BssNI | GRCGYC | 1 cut(s) | 190 |
| BssSI | CACGAG | 1 cut(s) | 497 |
| BssT1I | CCWWGG | 2 cut(s) | 33, 59 |
| Bst1107I | GTATAC | 1 cut(s) | 2105 |
| Bst2BI | CACGAG | 1 cut(s) | 497 |
| Bst2UI | CCWGG | 3 cut(s) | 365, 1195, 1251 |
| Bst4CI | ACNGT | 6 cut(s) | 658, 1216, 1438, 1512, 1875, 2074 |
| Bst6I | CTCTTC | 3 cut(s) | 219, 1053, 1107 |
| BstACI | GRCGYC | 1 cut(s) | 190 |
| BstAFI | CTTAAG | 1 cut(s) | 821 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 302 |
| BstBAI | YACGTR | 1 cut(s) | 869 |
| BstC8I | GCNNGC | 2 cut(s) | 1102, 1149 |
| BstDEI | CTNAG | 6 cut(s) | 633, 1478, 1593, 1690, 1695, 1832 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 88 |
| BstF5I | GGATG | 7 cut(s) | 748, 924, 982, 1347, 2107, 2127, 2269 |
| BstFNI | CGCG | 1 cut(s) | 1786 |
| BstH2I | RGCGCY | 1 cut(s) | 193 |
| BstHHI | GCGC | 2 cut(s) | 192, 1786 |
| BstKTI | GATC | 8 cut(s) | 175, 253, 853, 1065, 1645, 1819, 2063, 2205 |
| BstMAI | GTCTC | 1 cut(s) | 2182 |
| BstMBI | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| BstNI | CCWGG | 3 cut(s) | 365, 1195, 1251 |
| BstNSI | RCATGY | 1 cut(s) | 30 |
| BstSCI | CCNGG | 4 cut(s) | 363, 1022, 1193, 1249 |
| BstSFI | CTRYAG | 2 cut(s) | 724, 2001 |
| BstSLI | GKGCMC | 3 cut(s) | 536, 1621, 2169 |
| BstUI | CGCG | 1 cut(s) | 1786 |
| BstV1I | GCAGC | 8 cut(s) | 289, 308, 311, 343, 553, 1084, 1957, 2059 |
| BstV2I | GAAGAC | 3 cut(s) | 129, 222, 1735 |
| BstX2I | RGATCY | 2 cut(s) | 172, 2060 |
| BstYI | RGATCY | 2 cut(s) | 172, 2060 |
| BstZ17I | GTATAC | 1 cut(s) | 2105 |
| BsuRI | GGCC | 4 cut(s) | 503, 749, 1232, 1373 |
| BtrI | CACGTC | 1 cut(s) | 2142 |
| BtsCI | GGATG | 7 cut(s) | 748, 924, 982, 1347, 2107, 2127, 2269 |
| BtsI | GCAGTG | 1 cut(s) | 990 |
| BtsIMutI | CAGTG | 4 cut(s) | 990, 1221, 1443, 1880 |
| BveI | ACCTGC | 2 cut(s) | 1152, 1543 |
| Cac8I | GCNNGC | 2 cut(s) | 1102, 1149 |
| CaiI | CAGNNNCTG | 1 cut(s) | 302 |
| CciI | TCATGA | 1 cut(s) | 2155 |
| CfoI | GCGC | 2 cut(s) | 192, 1786 |
| Cfr10I | RCCGGY | 3 cut(s) | 1727, 2021, 2244 |
| Cfr13I | GGNCC | 4 cut(s) | 452, 747, 1230, 1372 |
| Csp6I | GTAC | 4 cut(s) | 68, 1133, 1448, 2258 |
| CspAI | ACCGGT | 2 cut(s) | 1727, 2021 |
| CviQI | GTAC | 4 cut(s) | 68, 1133, 1448, 2258 |
| DdeI | CTNAG | 6 cut(s) | 633, 1478, 1593, 1690, 1695, 1832 |
| DinI | GGCGCC | 1 cut(s) | 191 |
| DpnI | GATC | 8 cut(s) | 174, 252, 852, 1064, 1644, 1818, 2062, 2204 |
| DpnII | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| Eam1104I | CTCTTC | 3 cut(s) | 219, 1053, 1107 |
| EarI | CTCTTC | 3 cut(s) | 219, 1053, 1107 |
| EciI | GGCGGA | 2 cut(s) | 931, 1643 |
| Ecl136II | GAGCTC | 1 cut(s) | 1972 |
| Eco130I | CCWWGG | 2 cut(s) | 33, 59 |
| Eco24I | GRGCYC | 3 cut(s) | 162, 1179, 1974 |
| Eco32I | GATATC | 1 cut(s) | 775 |
| Eco47I | GGWCC | 1 cut(s) | 452 |
| Eco53kI | GAGCTC | 1 cut(s) | 1972 |
| Eco57I | CTGAAG | 2 cut(s) | 1131, 1560 |
| Eco88I | CYCGRG | 2 cut(s) | 602, 2116 |
| EcoICRI | GAGCTC | 1 cut(s) | 1972 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 88 |
| EcoRII | CCWGG | 3 cut(s) | 363, 1193, 1249 |
| EcoRV | GATATC | 1 cut(s) | 775 |
| EcoT14I | CCWWGG | 2 cut(s) | 33, 59 |
| EcoT22I | ATGCAT | 1 cut(s) | 1849 |
| EcoT38I | GRGCYC | 3 cut(s) | 162, 1179, 1974 |
| EgeI | GGCGCC | 1 cut(s) | 191 |
| EheI | GGCGCC | 1 cut(s) | 191 |
| ErhI | CCWWGG | 2 cut(s) | 33, 59 |
| FauI | CCCGC | 1 cut(s) | 60 |
| FauNDI | CATATG | 1 cut(s) | 1647 |
| FbaI | TGATCA | 1 cut(s) | 850 |
| FblI | GTMKAC | 1 cut(s) | 2104 |
| Fnu4HI | GCNGC | 8 cut(s) | 297, 300, 303, 332, 567, 1098, 1946, 2048 |
| FokI | GGATG | 6 cut(s) | 755, 931, 989, 1354, 2094, 2134 |
| FriOI | GRGCYC | 3 cut(s) | 162, 1179, 1974 |
| Fsp4HI | GCNGC | 8 cut(s) | 297, 300, 303, 332, 567, 1098, 1946, 2048 |
| FspBI | CTAG | 6 cut(s) | 60, 1577, 1608, 1656, 1902, 1985 |
| GlaI | GCGC | 2 cut(s) | 191, 1785 |
| GluI | GCNGC | 8 cut(s) | 297, 300, 303, 332, 567, 1098, 1946, 2048 |
| GsaI | CCCAGC | 1 cut(s) | 165 |
| GsuI | CTGGAG | 2 cut(s) | 347, 2270 |
| HaeII | RGCGCY | 1 cut(s) | 193 |
| HaeIII | GGCC | 4 cut(s) | 503, 749, 1232, 1373 |
| HapII | CCGG | 8 cut(s) | 193, 504, 1023, 1728, 2022, 2175, 2210, 2245 |
| HhaI | GCGC | 2 cut(s) | 192, 1786 |
| Hin1I | GRCGYC | 1 cut(s) | 190 |
| Hin6I | GCGC | 2 cut(s) | 190, 1784 |
| HinP1I | GCGC | 2 cut(s) | 190, 1784 |
| HindIII | AAGCTT | 2 cut(s) | 106, 1937 |
| HinfI | GANTC | 5 cut(s) | 46, 208, 430, 1736, 2087 |
| HpaII | CCGG | 8 cut(s) | 193, 504, 1023, 1728, 2022, 2175, 2210, 2245 |
| HphI | GGTGA | 7 cut(s) | 296, 716, 1039, 1610, 1711, 1966, 2082 |
| Hpy166II | GTNNAC | 6 cut(s) | 404, 534, 707, 1619, 1840, 2105 |
| Hpy188I | TCNGA | 7 cut(s) | 45, 157, 172, 346, 634, 649, 842 |
| Hpy188III | TCNNGA | 8 cut(s) | 176, 212, 401, 573, 1883, 1931, 2137, 2156 |
| Hpy8I | GTNNAC | 6 cut(s) | 404, 534, 707, 1619, 1840, 2105 |
| Hpy99I | CGWCG | 1 cut(s) | 2143 |
| HpyAV | CCTTC | 5 cut(s) | 94, 639, 1192, 1951, 2002 |
| HpyCH4III | ACNGT | 6 cut(s) | 658, 1216, 1438, 1512, 1875, 2074 |
| HpyCH4IV | ACGT | 3 cut(s) | 11, 868, 2141 |
| HpyF3I | CTNAG | 6 cut(s) | 633, 1478, 1593, 1690, 1695, 1832 |
| HpySE526I | ACGT | 3 cut(s) | 11, 868, 2141 |
| Hsp92I | GRCGYC | 1 cut(s) | 190 |
| HspAI | GCGC | 2 cut(s) | 190, 1784 |
| KasI | GGCGCC | 1 cut(s) | 189 |
| Ksp22I | TGATCA | 1 cut(s) | 850 |
| Kzo9I | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| LguI | GCTCTTC | 1 cut(s) | 1107 |
| LmnI | GCTCC | 8 cut(s) | 157, 346, 366, 892, 1190, 1996, 2139, 2236 |
| Lsp1109I | GCAGC | 8 cut(s) | 289, 308, 311, 343, 553, 1084, 1957, 2059 |
| LweI | GCATC | 4 cut(s) | 283, 667, 1498, 1762 |
| MaeI | CTAG | 6 cut(s) | 60, 1577, 1608, 1656, 1902, 1985 |
| MaeII | ACGT | 3 cut(s) | 11, 868, 2141 |
| MaeIII | GTNAC | 4 cut(s) | 864, 929, 1880, 2194 |
| MalI | GATC | 8 cut(s) | 174, 252, 852, 1064, 1644, 1818, 2062, 2204 |
| MboI | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| MfeI | CAATTG | 3 cut(s) | 407, 1082, 1674 |
| MflI | RGATCY | 2 cut(s) | 172, 2060 |
| MhlI | GDGCHC | 7 cut(s) | 162, 536, 1179, 1621, 1974, 2136, 2169 |
| Mly113I | GGCGCC | 1 cut(s) | 190 |
| MlyI | GAGTC | 1 cut(s) | 217 |
| MmeI | TCCRAC | 1 cut(s) | 479 |
| Mph1103I | ATGCAT | 1 cut(s) | 1849 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1202 |
| MseI | TTAA | 7 cut(s) | 104, 258, 791, 822, 1298, 1500, 2272 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1770, 1876 |
| MspA1I | CMGCKG | 2 cut(s) | 302, 1694 |
| MspCI | CTTAAG | 1 cut(s) | 821 |
| MspI | CCGG | 8 cut(s) | 193, 504, 1023, 1728, 2022, 2175, 2210, 2245 |
| MspR9I | CCNGG | 4 cut(s) | 365, 1024, 1195, 1251 |
| MunI | CAATTG | 3 cut(s) | 407, 1082, 1674 |
| MvaI | CCWGG | 3 cut(s) | 365, 1195, 1251 |
| MvnI | CGCG | 1 cut(s) | 1786 |
| NarI | GGCGCC | 1 cut(s) | 190 |
| NciI | CCSGG | 1 cut(s) | 1024 |
| NdeI | CATATG | 1 cut(s) | 1647 |
| NdeII | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| NlaIV | GGNNCC | 8 cut(s) | 159, 191, 748, 1192, 1231, 1374, 1998, 2166 |
| NmeAIII | GCCGAG | 1 cut(s) | 1177 |
| NmuCI | GTSAC | 2 cut(s) | 929, 1880 |
| NsiI | ATGCAT | 1 cut(s) | 1849 |
| NspI | RCATGY | 1 cut(s) | 30 |
| OliI | CACNNNNGTG | 1 cut(s) | 1876 |
| PagI | TCATGA | 1 cut(s) | 2155 |
| PaqCI | CACCTGC | 1 cut(s) | 1543 |
| PasI | CCCWGGG | 1 cut(s) | 1250 |
| PciSI | GCTCTTC | 1 cut(s) | 1107 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1202 |
| PfeI | GAWTC | 4 cut(s) | 46, 430, 1736, 2087 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1286 |
| PfoI | TCCNGGA | 1 cut(s) | 363 |
| PinAI | ACCGGT | 2 cut(s) | 1727, 2021 |
| PkrI | GCNGC | 8 cut(s) | 298, 301, 304, 333, 568, 1099, 1947, 2049 |
| PleI | GAGTC | 1 cut(s) | 216 |
| PluTI | GGCGCC | 1 cut(s) | 193 |
| PpsI | GAGTC | 1 cut(s) | 216 |
| Ppu21I | YACGTR | 1 cut(s) | 869 |
| PsiI | TTATAA | 1 cut(s) | 1275 |
| Psp124BI | GAGCTC | 1 cut(s) | 1974 |
| Psp1406I | AACGTT | 1 cut(s) | 11 |
| Psp6I | CCWGG | 3 cut(s) | 363, 1193, 1249 |
| PspFI | CCCAGC | 1 cut(s) | 161 |
| PspGI | CCWGG | 3 cut(s) | 363, 1193, 1249 |
| PspN4I | GGNNCC | 8 cut(s) | 159, 191, 748, 1192, 1231, 1374, 1998, 2166 |
| PspPI | GGNCC | 4 cut(s) | 452, 747, 1230, 1372 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 256, 288 |
| PstNI | CAGNNNCTG | 1 cut(s) | 302 |
| PsuI | RGATCY | 2 cut(s) | 172, 2060 |
| PvuII | CAGCTG | 2 cut(s) | 302, 1694 |
| RsaI | GTAC | 4 cut(s) | 69, 1134, 1449, 2259 |
| RsaNI | GTAC | 4 cut(s) | 68, 1133, 1448, 2258 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1770, 1876 |
| SacI | GAGCTC | 1 cut(s) | 1974 |
| SapI | GCTCTTC | 1 cut(s) | 1107 |
| SaqAI | TTAA | 7 cut(s) | 104, 258, 791, 822, 1298, 1500, 2272 |
| SatI | GCNGC | 8 cut(s) | 297, 300, 303, 332, 567, 1098, 1946, 2048 |
| Sau3AI | GATC | 8 cut(s) | 172, 250, 850, 1062, 1642, 1816, 2060, 2202 |
| Sau96I | GGNCC | 4 cut(s) | 452, 747, 1230, 1372 |
| SchI | GAGTC | 1 cut(s) | 217 |
| ScrFI | CCNGG | 4 cut(s) | 365, 1024, 1195, 1251 |
| SduI | GDGCHC | 7 cut(s) | 162, 536, 1179, 1621, 1974, 2136, 2169 |
| SfaNI | GCATC | 4 cut(s) | 283, 667, 1498, 1762 |
| SfcI | CTRYAG | 2 cut(s) | 724, 2001 |
| SfoI | GGCGCC | 1 cut(s) | 191 |
| SinI | GGWCC | 1 cut(s) | 452 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1770, 1876 |
| SmlI | CTYRAG | 4 cut(s) | 88, 821, 1185, 1940 |
| SmoI | CTYRAG | 4 cut(s) | 88, 821, 1185, 1940 |
| SsiI | CCGC | 5 cut(s) | 53, 916, 1628, 1661, 2234 |
| SspDI | GGCGCC | 1 cut(s) | 189 |
| SspMI | CTAG | 6 cut(s) | 60, 1577, 1608, 1656, 1902, 1985 |
| SstI | GAGCTC | 1 cut(s) | 1974 |
| StyD4I | CCNGG | 4 cut(s) | 363, 1022, 1193, 1249 |
| StyI | CCWWGG | 2 cut(s) | 33, 59 |
| TaaI | ACNGT | 6 cut(s) | 658, 1216, 1438, 1512, 1875, 2074 |
| TaiI | ACGT | 3 cut(s) | 14, 871, 2144 |
| TaqI | TCGA | 4 cut(s) | 414, 805, 1065, 1750 |
| TatI | WGTACW | 2 cut(s) | 1447, 2257 |
| TfiI | GAWTC | 4 cut(s) | 46, 430, 1736, 2087 |
| Tru1I | TTAA | 7 cut(s) | 104, 258, 791, 822, 1298, 1500, 2272 |
| Tru9I | TTAA | 7 cut(s) | 104, 258, 791, 822, 1298, 1500, 2272 |
| TscAI | CASTG | 4 cut(s) | 997, 1221, 1443, 1880 |
| TseFI | GTSAC | 2 cut(s) | 929, 1880 |
| TseI | GCWGC | 8 cut(s) | 296, 299, 302, 331, 566, 1097, 1945, 2047 |
| Tsp45I | GTSAC | 2 cut(s) | 929, 1880 |
| TspDTI | ATGAA | 9 cut(s) | 17, 237, 336, 708, 965, 1071, 1152, 1787, 2069 |
| TspGWI | ACGGA | 1 cut(s) | 2090 |
| TspRI | CASTG | 4 cut(s) | 997, 1221, 1443, 1880 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1286 |
| Vha464I | CTTAAG | 1 cut(s) | 821 |
| VneI | GTGCAC | 2 cut(s) | 532, 1617 |
| VpaK11BI | GGWCC | 1 cut(s) | 452 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 88 |
| XapI | RAATTY | 1 cut(s) | 1861 |
| XceI | RCATGY | 1 cut(s) | 30 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1449, 2119 |
| XmaJI | CCTAGG | 1 cut(s) | 59 |
| XmiI | GTMKAC | 1 cut(s) | 2104 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1202 |
| XspI | CTAG | 6 cut(s) | 60, 1577, 1608, 1656, 1902, 1985 |
| Zsp2I | ATGCAT | 1 cut(s) | 1849 |