Pre-mRNA-splicing factor ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 510, 1032 |
| Acc16I | TGCGCA | 1 cut(s) | 1022 |
| Acc65I | GGTACC | 1 cut(s) | 536 |
| AccB1I | GGYRCC | 1 cut(s) | 536 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 638 |
| AccI | GTMKAC | 2 cut(s) | 614, 660 |
| AciI | CCGC | 6 cut(s) | 94, 192, 213, 428, 456, 930 |
| AclWI | GGATC | 1 cut(s) | 482 |
| AcoI | YGGCCR | 1 cut(s) | 808 |
| AcsI | RAATTY | 4 cut(s) | 143, 182, 359, 377 |
| AcuI | CTGAAG | 1 cut(s) | 299 |
| AdeI | CACNNNGTG | 1 cut(s) | 1079 |
| AfaI | GTAC | 2 cut(s) | 538, 902 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 412, 638 |
| AgsI | TTSAA | 2 cut(s) | 100, 827 |
| AjnI | CCWGG | 1 cut(s) | 596 |
| AluBI | AGCT | 6 cut(s) | 61, 80, 115, 751, 853, 1065 |
| AluI | AGCT | 6 cut(s) | 61, 80, 115, 751, 853, 1065 |
| Alw21I | GWGCWC | 1 cut(s) | 760 |
| Alw26I | GTCTC | 3 cut(s) | 112, 588, 1127 |
| AlwI | GGATC | 1 cut(s) | 482 |
| AoxI | GGCC | 3 cut(s) | 301, 603, 808 |
| ApeKI | GCWGC | 4 cut(s) | 17, 20, 950, 1018 |
| ApoI | RAATTY | 4 cut(s) | 143, 182, 359, 377 |
| Asp718I | GGTACC | 1 cut(s) | 536 |
| AspLEI | GCGC | 2 cut(s) | 72, 1023 |
| AspS9I | GGNCC | 2 cut(s) | 292, 603 |
| AsuC2I | CCSGG | 4 cut(s) | 290, 654, 762, 905 |
| AsuHPI | GGTGA | 1 cut(s) | 620 |
| AvaII | GGWCC | 1 cut(s) | 292 |
| BanI | GGYRCC | 1 cut(s) | 536 |
| BanII | GRGCYC | 1 cut(s) | 533 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 529, 561 |
| BbsI | GAAGAC | 1 cut(s) | 51 |
| Bbv12I | GWGCWC | 1 cut(s) | 760 |
| BbvI | GCAGC | 4 cut(s) | 29, 32, 937, 1005 |
| BccI | CCATC | 4 cut(s) | 274, 640, 862, 1101 |
| BceAI | ACGGC | 1 cut(s) | 795 |
| BciT130I | CCWGG | 1 cut(s) | 598 |
| BcnI | CCSGG | 4 cut(s) | 290, 654, 762, 905 |
| BcoDI | GTCTC | 3 cut(s) | 112, 588, 1127 |
| BfaI | CTAG | 1 cut(s) | 402 |
| BisI | GCNGC | 4 cut(s) | 18, 21, 951, 1019 |
| BlpI | GCTNAGC | 1 cut(s) | 686 |
| BlsI | GCNGC | 4 cut(s) | 19, 22, 952, 1020 |
| Bme1390I | CCNGG | 5 cut(s) | 290, 598, 654, 762, 905 |
| Bme18I | GGWCC | 1 cut(s) | 292 |
| BmgT120I | GGNCC | 2 cut(s) | 292, 603 |
| BmiI | GGNNCC | 3 cut(s) | 294, 532, 538 |
| BmrFI | CCNGG | 5 cut(s) | 290, 598, 654, 762, 905 |
| BmsI | GCATC | 5 cut(s) | 562, 826, 962, 970, 1105 |
| BpiI | GAAGAC | 1 cut(s) | 51 |
| BpmI | CTGGAG | 4 cut(s) | 290, 578, 619, 884 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 686 |
| BpuEI | CTTGAG | 2 cut(s) | 223, 487 |
| BpuMI | CCSGG | 4 cut(s) | 290, 654, 762, 905 |
| BsaBI | GATNNNNATC | 2 cut(s) | 915, 1119 |
| BsaWI | WCCGGW | 1 cut(s) | 533 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 48, 78, 288, 318, 592, 622 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 412, 638 |
| Bse118I | RCCGGY | 1 cut(s) | 605 |
| Bse1I | ACTGG | 1 cut(s) | 1007 |
| Bse3DI | GCAATG | 3 cut(s) | 331, 548, 673 |
| Bse8I | GATNNNNATC | 2 cut(s) | 915, 1119 |
| BseBI | CCWGG | 1 cut(s) | 598 |
| BseGI | GGATG | 6 cut(s) | 90, 266, 285, 751, 953, 1112 |
| BseJI | GATNNNNATC | 2 cut(s) | 915, 1119 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 412, 638 |
| BseMI | GCAATG | 3 cut(s) | 331, 548, 673 |
| BseMII | CTCAG | 1 cut(s) | 700 |
| BseNI | ACTGG | 1 cut(s) | 1007 |
| BseRI | GAGGAG | 1 cut(s) | 41 |
| BseXI | GCAGC | 4 cut(s) | 29, 32, 937, 1005 |
| BseYI | CCCAGC | 2 cut(s) | 371, 751 |
| BshFI | GGCC | 3 cut(s) | 303, 605, 810 |
| BshNI | GGYRCC | 1 cut(s) | 536 |
| BsiHKAI | GWGCWC | 1 cut(s) | 760 |
| BsiSI | CCGG | 6 cut(s) | 290, 534, 606, 654, 762, 904 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 412, 638 |
| BsmAI | GTCTC | 3 cut(s) | 112, 588, 1127 |
| BsmBI | CGTCTC | 2 cut(s) | 112, 588 |
| BsmI | GAATGC | 1 cut(s) | 788 |
| BsnI | GGCC | 3 cut(s) | 303, 605, 810 |
| Bsp1286I | GDGCHC | 2 cut(s) | 533, 760 |
| Bsp143I | GATC | 2 cut(s) | 487, 910 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 686 |
| BspACI | CCGC | 6 cut(s) | 94, 192, 213, 428, 456, 930 |
| BspANI | GGCC | 3 cut(s) | 303, 605, 810 |
| BspCNI | CTCAG | 1 cut(s) | 699 |
| BspLI | GGNNCC | 3 cut(s) | 294, 532, 538 |
| BspPI | GGATC | 1 cut(s) | 482 |
| BspQI | GCTCTTC | 1 cut(s) | 429 |
| BspT107I | GGYRCC | 1 cut(s) | 536 |
| BsrDI | GCAATG | 3 cut(s) | 331, 548, 673 |
| BsrFI | RCCGGY | 1 cut(s) | 605 |
| BsrI | ACTGG | 1 cut(s) | 1007 |
| BssAI | RCCGGY | 1 cut(s) | 605 |
| BssMI | GATC | 2 cut(s) | 487, 910 |
| BssNAI | GTATAC | 1 cut(s) | 661 |
| Bst1107I | GTATAC | 1 cut(s) | 661 |
| Bst2UI | CCWGG | 1 cut(s) | 598 |
| Bst4CI | ACNGT | 2 cut(s) | 31, 541 |
| Bst6I | CTCTTC | 1 cut(s) | 429 |
| BstC8I | GCNNGC | 1 cut(s) | 332 |
| BstDEI | CTNAG | 2 cut(s) | 111, 686 |
| BstF5I | GGATG | 6 cut(s) | 90, 266, 285, 751, 953, 1112 |
| BstHHI | GCGC | 2 cut(s) | 72, 1023 |
| BstKTI | GATC | 2 cut(s) | 490, 913 |
| BstMAI | GTCTC | 3 cut(s) | 112, 588, 1127 |
| BstMBI | GATC | 2 cut(s) | 487, 910 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 67, 102, 528, 859 |
| BstNI | CCWGG | 1 cut(s) | 598 |
| BstNSI | RCATGY | 1 cut(s) | 790 |
| BstSCI | CCNGG | 5 cut(s) | 288, 596, 652, 760, 903 |
| BstV1I | GCAGC | 4 cut(s) | 29, 32, 937, 1005 |
| BstV2I | GAAGAC | 1 cut(s) | 51 |
| BstZ17I | GTATAC | 1 cut(s) | 661 |
| BsuRI | GGCC | 3 cut(s) | 303, 605, 810 |
| BtsCI | GGATG | 6 cut(s) | 90, 266, 285, 751, 953, 1112 |
| Cac8I | GCNNGC | 1 cut(s) | 332 |
| CfoI | GCGC | 2 cut(s) | 72, 1023 |
| Cfr10I | RCCGGY | 1 cut(s) | 605 |
| Cfr13I | GGNCC | 2 cut(s) | 292, 603 |
| CseI | GACGC | 1 cut(s) | 94 |
| Csp6I | GTAC | 2 cut(s) | 537, 901 |
| CviAII | CATG | 2 cut(s) | 787, 1024 |
| CviQI | GTAC | 2 cut(s) | 537, 901 |
| DdeI | CTNAG | 2 cut(s) | 111, 686 |
| DpnI | GATC | 2 cut(s) | 489, 912 |
| DpnII | GATC | 2 cut(s) | 487, 910 |
| DraIII | CACNNNGTG | 1 cut(s) | 1079 |
| EaeI | YGGCCR | 1 cut(s) | 808 |
| Eam1104I | CTCTTC | 1 cut(s) | 429 |
| EarI | CTCTTC | 1 cut(s) | 429 |
| EciI | GGCGGA | 2 cut(s) | 181, 417 |
| Eco147I | AGGCCT | 1 cut(s) | 303 |
| Eco24I | GRGCYC | 1 cut(s) | 533 |
| Eco47I | GGWCC | 1 cut(s) | 292 |
| Eco57I | CTGAAG | 1 cut(s) | 299 |
| EcoRII | CCWGG | 1 cut(s) | 596 |
| EcoT22I | ATGCAT | 1 cut(s) | 788 |
| EcoT38I | GRGCYC | 1 cut(s) | 533 |
| Esp3I | CGTCTC | 2 cut(s) | 112, 588 |
| FaeI | CATG | 2 cut(s) | 790, 1027 |
| FatI | CATG | 2 cut(s) | 786, 1023 |
| FauI | CCCGC | 2 cut(s) | 463, 937 |
| FauNDI | CATATG | 1 cut(s) | 621 |
| FblI | GTMKAC | 2 cut(s) | 614, 660 |
| Fnu4HI | GCNGC | 4 cut(s) | 18, 21, 951, 1019 |
| FokI | GGATG | 6 cut(s) | 77, 253, 272, 758, 940, 1119 |
| FriOI | GRGCYC | 1 cut(s) | 533 |
| Fsp4HI | GCNGC | 4 cut(s) | 18, 21, 951, 1019 |
| FspBI | CTAG | 1 cut(s) | 402 |
| FspI | TGCGCA | 1 cut(s) | 1022 |
| GlaI | GCGC | 2 cut(s) | 71, 1022 |
| GluI | GCNGC | 4 cut(s) | 18, 21, 951, 1019 |
| GsaI | CCCAGC | 2 cut(s) | 375, 755 |
| GsuI | CTGGAG | 4 cut(s) | 290, 578, 619, 884 |
| HaeIII | GGCC | 3 cut(s) | 303, 605, 810 |
| HapII | CCGG | 6 cut(s) | 290, 534, 606, 654, 762, 904 |
| HgaI | GACGC | 1 cut(s) | 94 |
| HhaI | GCGC | 2 cut(s) | 72, 1023 |
| Hin1II | CATG | 2 cut(s) | 790, 1027 |
| Hin6I | GCGC | 2 cut(s) | 70, 1021 |
| HinP1I | GCGC | 2 cut(s) | 70, 1021 |
| HindIII | AAGCTT | 2 cut(s) | 113, 851 |
| HinfI | GANTC | 7 cut(s) | 152, 310, 408, 611, 676, 800, 1003 |
| HpaII | CCGG | 6 cut(s) | 290, 534, 606, 654, 762, 904 |
| HphI | GGTGA | 1 cut(s) | 620 |
| Hpy166II | GTNNAC | 3 cut(s) | 615, 661, 709 |
| Hpy188I | TCNGA | 2 cut(s) | 167, 415 |
| Hpy188III | TCNNGA | 5 cut(s) | 202, 307, 466, 557, 977 |
| Hpy8I | GTNNAC | 3 cut(s) | 615, 661, 709 |
| HpyAV | CCTTC | 8 cut(s) | 205, 254, 501, 555, 719, 772, 868, 1104 |
| HpyCH4III | ACNGT | 2 cut(s) | 31, 541 |
| HpyCH4IV | ACGT | 1 cut(s) | 692 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 324, 553, 666, 786, 817, 953, 983 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 67, 102, 528, 859 |
| HpyF3I | CTNAG | 2 cut(s) | 111, 686 |
| HpySE526I | ACGT | 1 cut(s) | 692 |
| Hsp92II | CATG | 2 cut(s) | 790, 1027 |
| HspAI | GCGC | 2 cut(s) | 70, 1021 |
| KpnI | GGTACC | 1 cut(s) | 540 |
| Kzo9I | GATC | 2 cut(s) | 487, 910 |
| LguI | GCTCTTC | 1 cut(s) | 429 |
| LmnI | GCTCC | 2 cut(s) | 536, 763 |
| Lsp1109I | GCAGC | 4 cut(s) | 29, 32, 937, 1005 |
| LweI | GCATC | 5 cut(s) | 562, 826, 962, 970, 1105 |
| MaeI | CTAG | 1 cut(s) | 402 |
| MaeII | ACGT | 1 cut(s) | 692 |
| MaeIII | GTNAC | 1 cut(s) | 35 |
| MalI | GATC | 2 cut(s) | 489, 912 |
| MboI | GATC | 2 cut(s) | 487, 910 |
| MboII | GAAGA | 4 cut(s) | 56, 190, 305, 416 |
| MfeI | CAATTG | 1 cut(s) | 636 |
| MhlI | GDGCHC | 2 cut(s) | 533, 760 |
| MluCI | AATT | 8 cut(s) | 143, 182, 230, 280, 359, 377, 417, 636 |
| MlyI | GAGTC | 3 cut(s) | 146, 620, 809 |
| MmeI | TCCRAC | 1 cut(s) | 1084 |
| Mph1103I | ATGCAT | 1 cut(s) | 788 |
| MseI | TTAA | 2 cut(s) | 180, 915 |
| MslI | CAYNNNNRTG | 1 cut(s) | 510 |
| MspI | CCGG | 6 cut(s) | 290, 534, 606, 654, 762, 904 |
| MspR9I | CCNGG | 5 cut(s) | 290, 598, 654, 762, 905 |
| MunI | CAATTG | 1 cut(s) | 636 |
| Mva1269I | GAATGC | 1 cut(s) | 788 |
| MvaI | CCWGG | 1 cut(s) | 598 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 67, 102, 528, 859 |
| NciI | CCSGG | 4 cut(s) | 290, 654, 762, 905 |
| NdeI | CATATG | 1 cut(s) | 621 |
| NdeII | GATC | 2 cut(s) | 487, 910 |
| NlaIII | CATG | 2 cut(s) | 790, 1027 |
| NlaIV | GGNNCC | 3 cut(s) | 294, 532, 538 |
| NmuCI | GTSAC | 1 cut(s) | 35 |
| NsbI | TGCGCA | 1 cut(s) | 1022 |
| NsiI | ATGCAT | 1 cut(s) | 788 |
| NspI | RCATGY | 1 cut(s) | 790 |
| PceI | AGGCCT | 1 cut(s) | 303 |
| PciSI | GCTCTTC | 1 cut(s) | 429 |
| PctI | GAATGC | 1 cut(s) | 788 |
| PfeI | GAWTC | 4 cut(s) | 310, 408, 676, 1003 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 638 |
| PfoI | TCCNGGA | 1 cut(s) | 288 |
| PkrI | GCNGC | 4 cut(s) | 19, 22, 952, 1020 |
| PleI | GAGTC | 3 cut(s) | 146, 619, 808 |
| PpsI | GAGTC | 3 cut(s) | 146, 619, 808 |
| PsiI | TTATAA | 2 cut(s) | 510, 1032 |
| Psp6I | CCWGG | 1 cut(s) | 596 |
| PspFI | CCCAGC | 2 cut(s) | 371, 751 |
| PspGI | CCWGG | 1 cut(s) | 596 |
| PspN4I | GGNNCC | 3 cut(s) | 294, 532, 538 |
| PspPI | GGNCC | 2 cut(s) | 292, 603 |
| RsaI | GTAC | 2 cut(s) | 538, 902 |
| RsaNI | GTAC | 2 cut(s) | 537, 901 |
| RseI | CAYNNNNRTG | 1 cut(s) | 510 |
| SapI | GCTCTTC | 1 cut(s) | 429 |
| SaqAI | TTAA | 2 cut(s) | 180, 915 |
| SatI | GCNGC | 4 cut(s) | 18, 21, 951, 1019 |
| Sau3AI | GATC | 2 cut(s) | 487, 910 |
| Sau96I | GGNCC | 2 cut(s) | 292, 603 |
| SchI | GAGTC | 3 cut(s) | 146, 620, 809 |
| ScrFI | CCNGG | 5 cut(s) | 290, 598, 654, 762, 905 |
| SduI | GDGCHC | 2 cut(s) | 533, 760 |
| SfaNI | GCATC | 5 cut(s) | 562, 826, 962, 970, 1105 |
| SinI | GGWCC | 1 cut(s) | 292 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 510 |
| SmlI | CTYRAG | 2 cut(s) | 202, 466 |
| SmoI | CTYRAG | 2 cut(s) | 202, 466 |
| Sse9I | AATT | 8 cut(s) | 143, 182, 230, 280, 359, 377, 417, 636 |
| SseBI | AGGCCT | 1 cut(s) | 303 |
| SsiI | CCGC | 6 cut(s) | 94, 192, 213, 428, 456, 930 |
| SspMI | CTAG | 1 cut(s) | 402 |
| StuI | AGGCCT | 1 cut(s) | 303 |
| StyD4I | CCNGG | 5 cut(s) | 288, 596, 652, 760, 903 |
| TaaI | ACNGT | 2 cut(s) | 31, 541 |
| TaiI | ACGT | 1 cut(s) | 695 |
| TaqI | TCGA | 1 cut(s) | 592 |
| TasI | AATT | 8 cut(s) | 143, 182, 230, 280, 359, 377, 417, 636 |
| TfiI | GAWTC | 4 cut(s) | 310, 408, 676, 1003 |
| Tru1I | TTAA | 2 cut(s) | 180, 915 |
| Tru9I | TTAA | 2 cut(s) | 180, 915 |
| TseFI | GTSAC | 1 cut(s) | 35 |
| TseI | GCWGC | 4 cut(s) | 17, 20, 950, 1018 |
| Tsp45I | GTSAC | 1 cut(s) | 35 |
| TspDTI | ATGAA | 8 cut(s) | 223, 274, 494, 537, 668, 738, 1027, 1078 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 638 |
| VpaK11BI | GGWCC | 1 cut(s) | 292 |
| XapI | RAATTY | 4 cut(s) | 143, 182, 359, 377 |
| XceI | RCATGY | 1 cut(s) | 790 |
| XmiI | GTMKAC | 2 cut(s) | 614, 660 |
| XspI | CTAG | 1 cut(s) | 402 |
| Zsp2I | ATGCAT | 1 cut(s) | 788 |