Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000463

Physical Location & Seq

Genomic Coordinates Reverse (-)

86663080 .. 86667635

Gene Structure 4556 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00149040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3732 bp

ATGGGGTGTGAGAGCAGCCTGAAGACATGGGTGTCTGATAAGTTAATAGCATTGCTCGGATACTCTAATCCGGTAGTTGTTCAGTATGTTATTGGATTAACTAAAAAAGCAAACTCCCAGGCTGGTTTGGTGGACAAGCTTGTCGAATTTGGATTTGTGTCATCGACTGAAACAGGGGCATTTGCTGCAGATATCTTTGCAAGAGTGTGTGGTGAAACATCTGGTTTGAAGCAATGCGGGAAACAAGAGAGGGGAGGTGTAATGCCGGTGAGGATGATGCAGCGGACCCATGTTCTGTTGGATTCAGATGATGATGGTGATGCGGAGAGATGTTCGGTTGAGGTTGTTTCTCATTCTGTGTCTGTAACAGTGGATTTCAATAAGAAAAGATTCAGGAAGAGGGCATTAGGTCAGAAAGATGGAGATGATGATGAGGTTATTGCAGAACGTGTTAAAAGGCGGAACTTGCCAGATAAAGATGATCATGAGGATGGTTCAGAGTCAGAAGAAGAGAGATTGCGTGATCATAGAGAAAGGGGAGAAATTGGAGCAAAGTATAAGGAAGAGGACGCAGCAGCCACGCGAAAAACTGACAAAGAAAAACTTAAGACGAAAGGGGGGGAAGAGGAGGCTGTTCGAAAAAACAGTGGTGATGTTGAAGGTTTAAGAAGAGCTTCAAGACAAGAATATCTAAAGGAAAGAGAGAGAAAGAAAATGGATGAAATGAGGGATGATGTAGAAGATGAACATTACTTATTTGAAGGTCTAGATGAGGCTGAAAATGTCACCGAGTACAGGATGCCAGATGCCTATGATGATGAGGAGCGCGGTGTTACTCAACAGAAGAGATTTTCTGTGGCTTTGCAGCGCTACAAGGACGACAGTTCTGGGGATAAAATGAACTCGTTTGGACAACAGAAATCTTGGGAAGATCAGCAACTTGGAAAAGCAACATTGGAATTTGGTTCAAAAAACAAAAAAAAAAGGGTATCTGATGAGTATGATTTTGTATATGAAGACCAGATTGAGTTCGTCAGGGGGTTGGTGATCGAGGATGATAAATATGACAAGTTTGAGGGTGATAAGCAACTGCAGTCCACAAAATTACTTGAGTCAAGGGAAAAAACCTTGAAGATGTTCCAGGAGGAGAGGAAAACTTTACCCATCTTCTCCTTTCATGATGAGTTGCTCCAAGCTGTTAAAGATCATCAGGTTCTTGTTATTGTTGGCGAAACTGGATCTGGCAAAACTACACAGATTCCCCAGTATTTACATGAGGCGGGATATACAAAGCATGGAAAGATTGGGTGCACACAGCCACGGCGAGTTGCTGCTATGAGTGTTGCTGCCAGGGTTTCTCAAGAAATGGGTGTCAAGCTTGGACATGAGGTAGGTTATTCTATTCGTTTTGAGGATTGCACGTCTGAAAAGACCGTTTTGAAATATATGACTGATGGAATGTTGTTGCGTGAATTTCTTGCTGAACCAGATTTGGCAAGCTATAGTGTGCTGATGGTGGATGAGGCCCATGAGAGAACACTCTCTACTGATATTCTATTTGCATTAGGAGGCGGTTTTCCTGTCGATATATACCACACAAAAGCACCAGAAGCTGATTACTTAGATGCTGCAATTGCTGCTGCAATTCAAATCCATGTCAGGGAACCACCTGGAGACATATTGGTCTTCCTCACAGGTCAAGAAGATATAGAAACAGCAAGCGAAACATTAAAGTACAGGACAAGTGGTCTCGGGAGAAATATATCAGAGCTGATTATCTGTCCCATATATGCAAACCTGCCTACTGAGCAGCAAGCAAAAATTTTTGAGCCCACACCTGAAGGGGCTAGAAAGGTTGTCCTTGCCACAAACATAGCTGAAACTTCTCTGACTATTGATGGGATCAAATATGTCATCGACTGTGGCTATTGCAAGATGAAGTCCTATAATCCAAGGACTGGAATGGAGTCATTGCAAGTCACTCCCATCTCAAAGGCATCAGCAAGGCAGAGGGCAGGTCGATCTGGTCGAACAGGCCCTCGTAAGTGTTTCCGGTTATATACTGTTGACAGTTATATTCATGAATTAGATGATACCACAATACCTGAAATACTAAGGACTAACCTGGCAAATGTTGTTCTTAGGCTTAAGAGCCTTGGTATCCATGACTTGATACATTTTGATTTTATGGATCCTCCACCATCGGAAGCATTACTAAAAGCCCTTGAACTGTTATTTGCTTTATCTGCATTGAATAAAATGGGAGAGTTGACTAAAGTTGGTAGGCGGATGTCAGAGTTTCCACTTGATCCAATGCTATCTAAGATGGTAGTTGCTTCTGACAGGTACAATTGCTCAAAAGAGATCATTTCTATTGCTGCCATGCTTTCTGTCGGTAATTCAATCTTTTATCGTCCAAAGGACAAACAAGTCTATGCTGACAATGCAAGACAGACTTTTCACACTGGGGACACAGGAGATCACATTGCCTTGCTTAATGTTTACAATACATGGACCGAAACAAACTACTCAAGTCAATGGTGTTACGAAAACTATATAAACGCTAGGAGCATGAAAAGAGCAAGAGATATCAGGGATCAACTTAAGAGACTCTTGGAGAGGGTCGAGATTGAGCTAACCTCAAATCTTGATGATTTAGAGTCTATAAAGAAGGCCATTACATCAGGTTTCTTCCCTCATTGTGCAAGGCTGCGAAAGAATGGATCTTATAAAATAGTCAAACATCAGCAGGTTGCCTCCATACACCCCAGCTCAGGCTTGTCACAGGAGTTTCCGACATGGGTTTTATACCATGAACTGGTGCTTACATCCAAGGAATACATGAGACAGGTAACAGAGTTGAAGCCCGAGTGGTTGCTGGAAATAGCTCCACATTATTACCAACTAAAGGATGTCGATGCTGCTTTGACCTTGAAGAAAACGTCTAATGGCAATGTAATTTTACACGGTGTGAAAAGAGTCTTGAGGGTGAGCTCTGTTTTGGTAGTAGCCTGCAAACCTGACCGCTGTGTGCTGAAGTTTCTAGTTGTTACCTCCTTTATGAATTCTACAACGAAGTATAGCCGTGATTATACATTGCATGAAAGACTTCCTAACGAAGTACTCAACAAGAAAATCTTATTCCTTACCCGTTTGGGAAACCTATCTGTTGGTCTGGGAGACCAAATCCGTAGATGCAGTTCTGTCCATAACATTGTTGAGAAGTTCATCAAGAAGCGTTACAGAAAGAAGTTGGACATGTTGACGAATGAAGAAGATGAACAAGAAGAGGAATATCTTCAAGCTAGGCAGAGAGGGGAGCAGGACACAGCAGCCATACAAAAGTACACAACGAAACGAAAGTTAACTAATTTACACGAAGACGAAGAGGAAGAGGAAGAGGAAGAGGAAGAGATCACATGGGTCAAACGACGAAAGACTTTGGAGCACATTGGCAAGGAAATCAAAGCCTTGAGGATAACTACAAGACAAGAATATGGTACGAGTTGCAATATAGATTTCGCAAGTCTTCCTCCAGATATTGTGCTTCTTATTCTGGATAAGTTGTTAGAACCCATTGACCATGCTCGTTTTGCTGTCGTCTGTAAGGAATGGCAGTCTCTTGCAAAACAGTATAATTGTGAAACGCAGCCCTGGGTTAAGGTCCTCCCTCCCATGCTATGGATCCCTACCCAATCTCGTGGATCCGATGACCCAATGACCATGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031047 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1243

Amino Acids

141.77

Weight (kDa)

6.0

Isoelectric Point (pI)

42.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	548 - 679	9e-13	Helicase conserved C-terminal domain
WHD_HA2	PF04408	741 - 769	1e-09	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	770 - 831	3e-11	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	889 - 966	2.6e-24	Oligonucleotide/oligosaccharide-binding (OB)-fold
F-box-like	PF12937	1174 - 1210	1.5e-06	F-box-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0000463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32490 AT1G32490 AT2G35340 AT2G35340 AT4G16680 AT4G16680
fragaria_vesca	FvH4_1g06760 FvH4_1g06761 FvH4_1g06770 FvH4_3g40380 FvH4_3g40450 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_5g14240 FvH4_7g01330 FvH4_7g01330
malus_domestica	MD02G1072400.v1.1 MD02G1072500.v1.1 MD02G1088100.v1.1 MD06G1200100.v1.1 MD07G1285300.v1.1 MD08G1042900.v1.1
prunus_persica	Prupe.5G201700_v2.0.a1 Prupe.5G201700_v2.0.a1 Prupe.6G014300_v2.0.a1 Prupe.6G159800_v2.0.a1 Prupe.7G201500_v2.0.a1 Prupe.8G103900_v2.0.a1 Prupe.8G104500_v2.0.a1
pyrus_communis	pycom02g05640 pycom02g05650 pycom14g17310
rosa_chinensis	RchiOBHm_Chr2g0092491 RchiOBHm_Chr2g0092541 RchiOBHm_Chr5g0073091 RchiOBHm_Chr6g0263651 RchiOBHm_Chr6g0263711 RchiOBHm_Chr7g0182681
rosa_laevigata	RLG00000005109 RLG00000014320 RLG00000014322 RLG00000014324 RLG00000014326 RLG00000014327 RLG00000016318 RLG00000016322 RLG00000036362
rosa_multiflora	Rmu_sc0000014.1_g000045 Rmu_sc0002174.1_g000001 Rmu_sc0003570.1_g000001 Rmu_sc0004296.1_g000005 Rmu_sc0004296.1_g000014 Rmu_sc0005592.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431160 Rroxscaffold_176G00431170 Rroxscaffold_2G00148990 Rroxscaffold_2G00149040 Rroxscaffold_3G00271070 Rroxscaffold_6G00409880 Rroxscaffold_7G00204020 Rroxscaffold_7G00204040 Rroxscaffold_7G00204070
rosa_rugosa	Rorug02G0027100 Rorug02G0027200 Rorug02G0027300 Rorug05G0421000 Rorug06G0449100
rosa_samantha	Rh2AG072900 Rh2AG073200 Rh2BG073500 Rh2BG074100 Rh2CG074700 Rh2DG071600 Rh2DG072300 Rh5AG478000 Rh5BG498700 Rh5DG510800 Rh6AG125300 Rh6BG121400 Rh6CG119800 Rh7AG052900 Rh7BG052500 Rh7CG054000 Rh7DG052300
rosa_wichuraiana	Rw0G005280 Rw2G005970 Rw2G006000 Rw5G044390 Rw6G010890 Rw7G004310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2730
AasI	GACNNNNNNGTC	4 cut(s)	31, 140, 1682, 2429
Acc36I	ACCTGC	3 cut(s)	1806, 2006, 2740
AccB7I	CCANNNNNTGG	3 cut(s)	1958, 2818, 3681
AccII	CGCG	2 cut(s)	583, 828
AciI	CCGC	9 cut(s)	237, 283, 323, 460, 828, 1280, 1572, 2287, 3025
AcsI	RAATTY	5 cut(s)	146, 959, 1472, 1821, 3064
AcuI	CTGAAG	3 cut(s)	41, 1860, 3056
AdeI	CACNNNGTG	1 cut(s)	2971
AfaI	GTAC	6 cut(s)	794, 1736, 2348, 3123, 3347, 3502
AfeI	AGCGCT	1 cut(s)	869
AfiI	CCNNNNNNNGG	7 cut(s)	795, 1660, 1958, 2774, 2818, 2908, 3681
AflII	CTTAAG	3 cut(s)	605, 2147, 2603
AflIII	ACRYGT	2 cut(s)	448, 3258
AhdI	GACNNNNNGTC	1 cut(s)	1746
AjiI	CACGTC	1 cut(s)	1422
AjnI	CCWGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
Alw21I	GWGCWC	3 cut(s)	1313, 2996, 3450
Alw26I	GTCTC	6 cut(s)	1668, 1754, 2602, 2839, 3174, 3624
Alw44I	GTGCAC	1 cut(s)	1309
AlwNI	CAGNNNCTG	1 cut(s)	1613
Ama87I	CYCGRG	2 cut(s)	1751, 2867
Aor51HI	AGCGCT	1 cut(s)	869
AoxI	GGCC	3 cut(s)	1524, 2035, 2673
ApaLI	GTGCAC	1 cut(s)	1309
ApoI	RAATTY	5 cut(s)	146, 959, 1472, 1821, 3064
Asp700I	GAANNNNTTC	5 cut(s)	389, 673, 1136, 3108, 3297
AspLEI	GCGC	2 cut(s)	828, 870
AspS9I	GGNCC	5 cut(s)	285, 1525, 2036, 2514, 3664
AsuHPI	GGTGA	8 cut(s)	224, 280, 329, 662, 778, 1057, 1091, 3001
AsuII	TTCGAA	1 cut(s)	637
AvaI	CYCGRG	2 cut(s)	1751, 2867
AvaII	GGWCC	3 cut(s)	285, 2514, 3664
BaeGI	GKGCMC	1 cut(s)	1313
BamHI	GGATCC	3 cut(s)	2191, 3684, 3704
BanII	GRGCYC	2 cut(s)	1833, 2996
BauI	CACGAG	1 cut(s)	3699
BbsI	GAAGAC	5 cut(s)	29, 1023, 1678, 3387, 3521
Bbv12I	GWGCWC	3 cut(s)	1313, 2996, 3450
BceAI	ACGGC	2 cut(s)	1337, 3069
BciT130I	CCWGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
BciVI	GTATCC	2 cut(s)	53, 2171
BclI	TGATCA	2 cut(s)	481, 523
BcoDI	GTCTC	6 cut(s)	1668, 1754, 2602, 2839, 3174, 3624
BfaI	CTAG	5 cut(s)	767, 1848, 2565, 3044, 3306
BfmI	CTRYAG	3 cut(s)	186, 1091, 1501
BfoI	RGCGCY	1 cut(s)	871
BfrI	CTTAAG	3 cut(s)	605, 2147, 2603
BfuAI	ACCTGC	3 cut(s)	1806, 2006, 2740
BfuI	GTATCC	2 cut(s)	53, 2171
BmcAI	AGTACT	1 cut(s)	3123
Bme1390I	CCNGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
Bme18I	GGWCC	3 cut(s)	285, 2514, 3664
BmeRI	GACNNNNNGTC	1 cut(s)	1746
BmeT110I	CYCGRG	2 cut(s)	1751, 2867
BmgBI	CACGTC	1 cut(s)	1422
BmgT120I	GGNCC	5 cut(s)	285, 1525, 2036, 2514, 3664
BmiI	GGNNCC	5 cut(s)	287, 1665, 2193, 3686, 3706
BmrFI	CCNGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
BmrI	ACTGGG	2 cut(s)	1258, 2475
BmsI	GCATC	8 cut(s)	267, 310, 789, 796, 1615, 2006, 2908, 3185
BmuI	ACTGGG	2 cut(s)	1258, 2475
BpiI	GAAGAC	5 cut(s)	29, 1023, 1678, 3387, 3521
BplI	GAGNNNNNCTC	2 cut(s)	2624, 2656
BpmI	CTGGAG	2 cut(s)	1692, 3519
Bpu10I	CCTNAGC	1 cut(s)	2773
Bpu14I	TTCGAA	1 cut(s)	637
BpuEI	CTTGAG	5 cut(s)	1130, 1344, 2515, 3004, 3493
BsaI	GGTCTC	2 cut(s)	1754, 3174
BsaJI	CCNNGG	8 cut(s)	117, 1319, 1350, 1952, 2155, 2832, 3653, 3654
BsaWI	WCCGGW	2 cut(s)	70, 2052
Bsc4I	CCNNNNNNNGG	7 cut(s)	795, 1660, 1958, 2774, 2818, 2908, 3681
Bse118I	RCCGGY	1 cut(s)	265
Bse1I	ACTGG	5 cut(s)	1240, 1264, 1963, 2470, 2823
Bse3DI	GCAATG	6 cut(s)	50, 239, 1970, 2484, 2959, 3095
BseBI	CCWGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
BseDI	CCNNGG	8 cut(s)	117, 1319, 1350, 1952, 2155, 2832, 3653, 3654
BseLI	CCNNNNNNNGG	7 cut(s)	795, 1660, 1958, 2774, 2818, 2908, 3681
BseMI	GCAATG	6 cut(s)	50, 239, 1970, 2484, 2959, 3095
BseMII	CTCAG	2 cut(s)	1797, 2787
BseNI	ACTGG	5 cut(s)	1240, 1264, 1963, 2470, 2823
BseRI	GAGGAG	3 cut(s)	641, 836, 1160
BseSI	GKGCMC	1 cut(s)	1313
BseYI	CCCAGC	1 cut(s)	2768
Bsh1236I	CGCG	2 cut(s)	583, 828
BshFI	GGCC	3 cut(s)	1526, 2037, 2675
BsiHKAI	GWGCWC	3 cut(s)	1313, 2996, 3450
BsiHKCI	CYCGRG	2 cut(s)	1751, 2867
BsiSI	CCGG	3 cut(s)	71, 266, 2053
BslFI	GGGAC	2 cut(s)	1767, 2483
BslI	CCNNNNNNNGG	7 cut(s)	795, 1660, 1958, 2774, 2818, 2908, 3681
BsmAI	GTCTC	6 cut(s)	1668, 1754, 2602, 2839, 3174, 3624
BsmFI	GGGAC	2 cut(s)	1767, 2483
BsnI	GGCC	3 cut(s)	1526, 2037, 2675
Bso31I	GGTCTC	2 cut(s)	1754, 3174
BsoBI	CYCGRG	2 cut(s)	1751, 2867
Bsp119I	TTCGAA	1 cut(s)	637
Bsp1286I	GDGCHC	4 cut(s)	1313, 1833, 2996, 3450
BspACI	CCGC	9 cut(s)	237, 283, 323, 460, 828, 1280, 1572, 2287, 3025
BspANI	GGCC	3 cut(s)	1526, 2037, 2675
BspCNI	CTCAG	2 cut(s)	1798, 2786
BspFNI	CGCG	2 cut(s)	583, 828
BspHI	TCATGA	3 cut(s)	484, 1177, 2080
BspLI	GGNNCC	5 cut(s)	287, 1665, 2193, 3686, 3706
BspMAI	CTGCAG	2 cut(s)	190, 1095
BspMI	ACCTGC	3 cut(s)	1806, 2006, 2740
BspQI	GCTCTTC	1 cut(s)	664
BspT104I	TTCGAA	1 cut(s)	637
BspTI	CTTAAG	3 cut(s)	605, 2147, 2603
BspTNI	GGTCTC	2 cut(s)	1754, 3174
BsrDI	GCAATG	6 cut(s)	50, 239, 1970, 2484, 2959, 3095
BsrFI	RCCGGY	1 cut(s)	265
BsrI	ACTGG	5 cut(s)	1240, 1264, 1963, 2470, 2823
BssAI	RCCGGY	1 cut(s)	265
BssECI	CCNNGG	8 cut(s)	117, 1319, 1350, 1952, 2155, 2832, 3653, 3654
BssSI	CACGAG	1 cut(s)	3699
BssT1I	CCWWGG	3 cut(s)	1952, 2155, 2832
Bst2BI	CACGAG	1 cut(s)	3699
Bst2UI	CCWGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
BstAFI	CTTAAG	3 cut(s)	605, 2147, 2603
BstAPI	GCANNNNNTGC	2 cut(s)	185, 1637
BstBI	TTCGAA	1 cut(s)	637
BstC8I	GCNNGC	4 cut(s)	1498, 1720, 1815, 3013
BstDEI	CTNAG	6 cut(s)	1621, 1806, 2114, 2141, 2322, 2773
BstDSI	CCRYGG	1 cut(s)	1319
BstFNI	CGCG	2 cut(s)	583, 828
BstH2I	RGCGCY	1 cut(s)	871
BstHHI	GCGC	2 cut(s)	828, 870
BstMAI	GTCTC	6 cut(s)	1668, 1754, 2602, 2839, 3174, 3624
BstMWI	GCNNNNNNNGC	9 cut(s)	185, 466, 1610, 1634, 1637, 1807, 2246, 2444, 3593
BstNI	CCWGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
BstNSI	RCATGY	1 cut(s)	3262
BstSCI	CCNGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
BstSFI	CTRYAG	3 cut(s)	186, 1091, 1501
BstSLI	GKGCMC	1 cut(s)	1313
BstUI	CGCG	2 cut(s)	583, 828
BstV2I	GAAGAC	5 cut(s)	29, 1023, 1678, 3387, 3521
BstX2I	RGATCY	5 cut(s)	1238, 2191, 2723, 3684, 3704
BstXI	CCANNNNNNTGG	1 cut(s)	3701
BstYI	RGATCY	5 cut(s)	1238, 2191, 2723, 3684, 3704
BsuI	GTATCC	2 cut(s)	53, 2171
BsuRI	GGCC	3 cut(s)	1526, 2037, 2675
BtgI	CCRYGG	1 cut(s)	1319
BtrI	CACGTC	1 cut(s)	1422
BtsIMutI	CAGTG	3 cut(s)	375, 652, 2463
BveI	ACCTGC	3 cut(s)	1806, 2006, 2740
Cac8I	GCNNGC	4 cut(s)	1498, 1720, 1815, 3013
CaiI	CAGNNNCTG	1 cut(s)	1613
CciI	TCATGA	3 cut(s)	484, 1177, 2080
CfoI	GCGC	2 cut(s)	828, 870
Cfr10I	RCCGGY	1 cut(s)	265
Cfr13I	GGNCC	5 cut(s)	285, 1525, 2036, 2514, 3664
CseI	GACGC	1 cut(s)	578
Csp6I	GTAC	6 cut(s)	793, 1735, 2347, 3122, 3346, 3501
CviQI	GTAC	6 cut(s)	793, 1735, 2347, 3122, 3346, 3501
DdeI	CTNAG	6 cut(s)	1621, 1806, 2114, 2141, 2322, 2773
DraIII	CACNNNGTG	1 cut(s)	2971
DrdI	GACNNNNNNGTC	4 cut(s)	31, 140, 1682, 2429
DriI	GACNNNNNGTC	1 cut(s)	1746
DseDI	GACNNNNNNGTC	4 cut(s)	31, 140, 1682, 2429
Eam1105I	GACNNNNNGTC	1 cut(s)	1746
EciI	GGCGGA	2 cut(s)	475, 2302
Ecl136II	GAGCTC	1 cut(s)	2994
Eco130I	CCWWGG	3 cut(s)	1952, 2155, 2832
Eco24I	GRGCYC	2 cut(s)	1833, 2996
Eco31I	GGTCTC	2 cut(s)	1754, 3174
Eco32I	GATATC	2 cut(s)	193, 2590
Eco47I	GGWCC	3 cut(s)	285, 2514, 3664
Eco47III	AGCGCT	1 cut(s)	869
Eco53kI	GAGCTC	1 cut(s)	2994
Eco57I	CTGAAG	3 cut(s)	41, 1860, 3056
Eco88I	CYCGRG	2 cut(s)	1751, 2867
EcoICRI	GAGCTC	1 cut(s)	2994
EcoO109I	RGGNCCY	2 cut(s)	2036, 3664
EcoRI	GAATTC	1 cut(s)	3064
EcoRII	CCWGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
EcoRV	GATATC	2 cut(s)	193, 2590
EcoT14I	CCWWGG	3 cut(s)	1952, 2155, 2832
EcoT38I	GRGCYC	2 cut(s)	1833, 2996
ErhI	CCWWGG	3 cut(s)	1952, 2155, 2832
FaqI	GGGAC	2 cut(s)	1767, 2483
FauI	CCCGC	2 cut(s)	230, 1273
FbaI	TGATCA	2 cut(s)	481, 523
FriOI	GRGCYC	2 cut(s)	1833, 2996
FspBI	CTAG	5 cut(s)	767, 1848, 2565, 3044, 3306
GlaI	GCGC	2 cut(s)	827, 869
GsaI	CCCAGC	1 cut(s)	2772
GsuI	CTGGAG	2 cut(s)	1692, 3519
HaeII	RGCGCY	1 cut(s)	871
HaeIII	GGCC	3 cut(s)	1526, 2037, 2675
HapII	CCGG	3 cut(s)	71, 266, 2053
HgaI	GACGC	1 cut(s)	578
HhaI	GCGC	2 cut(s)	828, 870
Hin6I	GCGC	2 cut(s)	826, 868
HinP1I	GCGC	2 cut(s)	826, 868
HincII	GTYRAC	4 cut(s)	2068, 2271, 3264, 3366
HindII	GTYRAC	4 cut(s)	2068, 2271, 3264, 3366
HindIII	AAGCTT	2 cut(s)	137, 1376
HinfI	GANTC	9 cut(s)	302, 390, 500, 1112, 1258, 1967, 2610, 2660, 2979
HpaI	GTTAAC	1 cut(s)	3366
HpaII	CCGG	3 cut(s)	71, 266, 2053
HphI	GGTGA	8 cut(s)	224, 280, 329, 662, 778, 1057, 1091, 3001
Hpy166II	GTNNAC	9 cut(s)	133, 1098, 1311, 2068, 2271, 2503, 3264, 3348, 3366
Hpy8I	GTNNAC	9 cut(s)	133, 1098, 1311, 2068, 2271, 2503, 3264, 3348, 3366
Hpy99I	CGWCG	1 cut(s)	3435
HpyAV	CCTTC	4 cut(s)	653, 755, 1835, 2665
HpyCH4IV	ACGT	3 cut(s)	448, 1421, 2942
HpyF10VI	GCNNNNNNNGC	9 cut(s)	185, 466, 1610, 1634, 1637, 1807, 2246, 2444, 3593
HpyF3I	CTNAG	6 cut(s)	1621, 1806, 2114, 2141, 2322, 2773
HpySE526I	ACGT	3 cut(s)	448, 1421, 2942
HspAI	GCGC	2 cut(s)	826, 868
Ksp22I	TGATCA	2 cut(s)	481, 523
KspAI	GTTAAC	1 cut(s)	3366
LguI	GCTCTTC	1 cut(s)	664
LmnI	GCTCC	7 cut(s)	548, 823, 1194, 2568, 2893, 3319, 3445
LweI	GCATC	8 cut(s)	267, 310, 789, 796, 1615, 2006, 2908, 3185
MaeI	CTAG	5 cut(s)	767, 1848, 2565, 3044, 3306
MaeII	ACGT	3 cut(s)	448, 1421, 2942
MaeIII	GTNAC	9 cut(s)	364, 784, 832, 1978, 2543, 2781, 2851, 3049, 3239
MfeI	CAATTG	2 cut(s)	1632, 2350
MflI	RGATCY	5 cut(s)	1238, 2191, 2723, 3684, 3704
MhlI	GDGCHC	4 cut(s)	1313, 1833, 2996, 3450
MlyI	GAGTC	6 cut(s)	509, 1121, 1976, 2604, 2669, 2988
MmeI	TCCRAC	3 cut(s)	279, 2819, 3234
MroXI	GAANNNNTTC	5 cut(s)	389, 673, 1136, 3108, 3297
MslI	CAYNNNNRTG	1 cut(s)	489
MspA1I	CMGCKG	2 cut(s)	283, 3027
MspCI	CTTAAG	3 cut(s)	605, 2147, 2603
MspI	CCGG	3 cut(s)	71, 266, 2053
MspR9I	CCNGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
MunI	CAATTG	2 cut(s)	1632, 2350
MvaI	CCWGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
MvnI	CGCG	2 cut(s)	583, 828
MwoI	GCNNNNNNNGC	9 cut(s)	185, 466, 1610, 1634, 1637, 1807, 2246, 2444, 3593
NlaIV	GGNNCC	5 cut(s)	287, 1665, 2193, 3686, 3706
NmuCI	GTSAC	3 cut(s)	784, 1978, 2781
NspI	RCATGY	1 cut(s)	3262
NspV	TTCGAA	1 cut(s)	637
PagI	TCATGA	3 cut(s)	484, 1177, 2080
PasI	CCCWGGG	1 cut(s)	3654
PciI	ACATGT	1 cut(s)	3258
PciSI	GCTCTTC	1 cut(s)	664
PcsI	WCGNNNNNNNCGW	1 cut(s)	2026
PdmI	GAANNNNTTC	5 cut(s)	389, 673, 1136, 3108, 3297
PfeI	GAWTC	3 cut(s)	302, 390, 1258
PflMI	CCANNNNNTGG	3 cut(s)	1958, 2818, 3681
PfoI	TCCNGGA	1 cut(s)	1140
PleI	GAGTC	6 cut(s)	508, 1120, 1975, 2604, 2668, 2987
PpsI	GAGTC	6 cut(s)	508, 1120, 1975, 2604, 2668, 2987
PpuMI	RGGWCCY	1 cut(s)	3664
PscI	ACATGT	1 cut(s)	3258
PsiI	TTATAA	1 cut(s)	2730
Psp124BI	GAGCTC	1 cut(s)	2996
Psp5II	RGGWCCY	1 cut(s)	3664
Psp6I	CCWGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
PspFI	CCCAGC	1 cut(s)	2768
PspGI	CCWGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
PspN4I	GGNNCC	5 cut(s)	287, 1665, 2193, 3686, 3706
PspPI	GGNCC	5 cut(s)	285, 1525, 2036, 2514, 3664
PspPPI	RGGWCCY	1 cut(s)	3664
PsrI	GAACNNNNNNTAC	4 cut(s)	1528, 1560, 3184, 3216
PstI	CTGCAG	2 cut(s)	190, 1095
PstNI	CAGNNNCTG	1 cut(s)	1613
PsuI	RGATCY	5 cut(s)	1238, 2191, 2723, 3684, 3704
RsaI	GTAC	6 cut(s)	794, 1736, 2348, 3123, 3347, 3502
RsaNI	GTAC	6 cut(s)	793, 1735, 2347, 3122, 3346, 3501
RseI	CAYNNNNRTG	1 cut(s)	489
SacI	GAGCTC	1 cut(s)	2996
SapI	GCTCTTC	1 cut(s)	664
Sau96I	GGNCC	5 cut(s)	285, 1525, 2036, 2514, 3664
ScaI	AGTACT	1 cut(s)	3123
SchI	GAGTC	6 cut(s)	509, 1121, 1976, 2604, 2669, 2988
ScrFI	CCNGG	6 cut(s)	119, 1142, 1351, 1671, 2126, 3655
SduI	GDGCHC	4 cut(s)	1313, 1833, 2996, 3450
SfaNI	GCATC	8 cut(s)	267, 310, 789, 796, 1615, 2006, 2908, 3185
SfcI	CTRYAG	3 cut(s)	186, 1091, 1501
SfuI	TTCGAA	1 cut(s)	637
SinI	GGWCC	3 cut(s)	285, 2514, 3664
SmiMI	CAYNNNNRTG	1 cut(s)	489
SmlI	CTYRAG	8 cut(s)	605, 1109, 1359, 2147, 2530, 2603, 2983, 3472
SmoI	CTYRAG	8 cut(s)	605, 1109, 1359, 2147, 2530, 2603, 2983, 3472
SsiI	CCGC	9 cut(s)	237, 283, 323, 460, 828, 1280, 1572, 2287, 3025
SspMI	CTAG	5 cut(s)	767, 1848, 2565, 3044, 3306
SstI	GAGCTC	1 cut(s)	2996
StyD4I	CCNGG	6 cut(s)	117, 1140, 1349, 1669, 2124, 3653
StyI	CCWWGG	3 cut(s)	1952, 2155, 2832
TaiI	ACGT	3 cut(s)	451, 1424, 2945
TaqII	GACCGA	1 cut(s)	2531
TatI	WGTACW	4 cut(s)	792, 1734, 3121, 3345
TfiI	GAWTC	3 cut(s)	302, 390, 1258
TscAI	CASTG	3 cut(s)	375, 652, 2470
TseFI	GTSAC	3 cut(s)	784, 1978, 2781
Tsp45I	GTSAC	3 cut(s)	784, 1978, 2781
TspGWI	ACGGA	1 cut(s)	3179
TspRI	CASTG	3 cut(s)	375, 652, 2470
Van91I	CCANNNNNTGG	3 cut(s)	1958, 2818, 3681
Vha464I	CTTAAG	3 cut(s)	605, 2147, 2603
VneI	GTGCAC	1 cut(s)	1309
VpaK11BI	GGWCC	3 cut(s)	285, 2514, 3664
XapI	RAATTY	5 cut(s)	146, 959, 1472, 1821, 3064
XbaI	TCTAGA	1 cut(s)	766
XceI	RCATGY	1 cut(s)	3262
XmnI	GAANNNNTTC	5 cut(s)	389, 673, 1136, 3108, 3297
XspI	CTAG	5 cut(s)	767, 1848, 2565, 3044, 3306
ZrmI	AGTACT	1 cut(s)	3123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.