Pre-mRNA-splicing factor ATP-dependent RNA helicase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DEAD | PF00270 | 364 - 515 | 4.6e-07 | DEAD/DEAH box helicase |
| Helicase_C | PF00271 | 563 - 689 | 5.3e-12 | Helicase conserved C-terminal domain |
| WHD_HA2 | PF04408 | 751 - 779 | 4.6e-11 | Helicase associated domain (HA2), winged-helix |
| HA2_C | PF21010 | 780 - 841 | 5.6e-10 | Helicase associated domain (HA2), ratchet-like |
| OB_NTP_bind | PF07717 | 899 - 976 | 2e-22 | Oligonucleotide/oligosaccharide-binding (OB)-fold |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2239 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2459 |
| Acc36I | ACCTGC | 2 cut(s) | 808, 2239 |
| AccBSI | CCGCTC | 1 cut(s) | 2638 |
| AccI | GTMKAC | 2 cut(s) | 696, 2314 |
| AccII | CGCG | 2 cut(s) | 1237, 1242 |
| AciI | CCGC | 5 cut(s) | 1235, 1237, 2045, 2638, 2921 |
| AclWI | GGATC | 7 cut(s) | 840, 1940, 2198, 2216, 2229, 2333, 2346 |
| AcoI | YGGCCR | 2 cut(s) | 120, 1141 |
| AcsI | RAATTY | 3 cut(s) | 397, 1553, 2485 |
| AcuI | CTGAAG | 5 cut(s) | 41, 369, 1890, 2256, 2352 |
| AcvI | CACGTG | 1 cut(s) | 1687 |
| AcyI | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| AfaI | GTAC | 5 cut(s) | 1082, 1199, 1361, 1672, 2397 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1988, 2804, 2805, 2938 |
| AgeI | ACCGGT | 1 cut(s) | 1148 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1776 |
| AjiI | CACGTC | 1 cut(s) | 27 |
| AjnI | CCWGG | 6 cut(s) | 1053, 1265, 1594, 1699, 1951, 2068 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 530, 562 |
| AleI | CACNNNNGTG | 1 cut(s) | 2569 |
| Alw21I | GWGCWC | 4 cut(s) | 808, 1229, 2387, 2667 |
| Alw26I | GTCTC | 2 cut(s) | 37, 2543 |
| Alw44I | GTGCAC | 2 cut(s) | 804, 1225 |
| AlwI | GGATC | 7 cut(s) | 840, 1940, 2198, 2216, 2229, 2333, 2346 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1183, 1643 |
| Ama87I | CYCGRG | 1 cut(s) | 2973 |
| AoxI | GGCC | 4 cut(s) | 120, 1141, 1845, 2065 |
| ApaLI | GTGCAC | 2 cut(s) | 804, 1225 |
| ApeKI | GCWGC | 7 cut(s) | 419, 1247, 1262, 1658, 1840, 2203, 2408 |
| ApoI | RAATTY | 3 cut(s) | 397, 1553, 2485 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2542, 2574 |
| AsiGI | ACCGGT | 1 cut(s) | 1148 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 505, 587 |
| AspA2I | CCTAGG | 1 cut(s) | 248 |
| AspLEI | GCGC | 3 cut(s) | 1092, 1242, 2992 |
| AspS9I | GGNCC | 3 cut(s) | 156, 2066, 2851 |
| AsuC2I | CCSGG | 1 cut(s) | 1498 |
| AsuHPI | GGTGA | 9 cut(s) | 55, 340, 827, 902, 1495, 2306, 2659, 2701, 2848 |
| AvaI | CYCGRG | 1 cut(s) | 2973 |
| AvaII | GGWCC | 2 cut(s) | 156, 2851 |
| AvrII | CCTAGG | 1 cut(s) | 248 |
| BaeGI | GKGCMC | 3 cut(s) | 808, 1229, 2281 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1163, 1196 |
| BalI | TGGCCA | 1 cut(s) | 1143 |
| BamHI | GGATCC | 2 cut(s) | 2221, 2338 |
| BanII | GRGCYC | 1 cut(s) | 2667 |
| BauI | CACGAG | 1 cut(s) | 1445 |
| BbrPI | CACGTG | 1 cut(s) | 1687 |
| BbsI | GAAGAC | 3 cut(s) | 29, 1352, 1612 |
| Bbv12I | GWGCWC | 4 cut(s) | 808, 1229, 2387, 2667 |
| BbvI | GCAGC | 7 cut(s) | 431, 1249, 1259, 1670, 1827, 2190, 2395 |
| BceAI | ACGGC | 1 cut(s) | 189 |
| BciT130I | CCWGG | 6 cut(s) | 1055, 1267, 1596, 1701, 1953, 2070 |
| BclI | TGATCA | 2 cut(s) | 478, 2626 |
| BcnI | CCSGG | 1 cut(s) | 1498 |
| BcoDI | GTCTC | 2 cut(s) | 37, 2543 |
| BfaI | CTAG | 8 cut(s) | 140, 249, 1530, 1781, 1833, 1878, 2273, 2736 |
| BfmI | CTRYAG | 4 cut(s) | 1417, 1841, 2080, 2679 |
| BfuAI | ACCTGC | 2 cut(s) | 808, 2239 |
| BlnI | CCTAGG | 1 cut(s) | 248 |
| BlpI | GCTNAGC | 1 cut(s) | 1797 |
| Bme1390I | CCNGG | 7 cut(s) | 1055, 1267, 1498, 1596, 1701, 1953, 2070 |
| Bme18I | GGWCC | 2 cut(s) | 156, 2851 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1776 |
| BmeT110I | CYCGRG | 1 cut(s) | 2973 |
| BmgBI | CACGTC | 1 cut(s) | 27 |
| BmgT120I | GGNCC | 3 cut(s) | 156, 2066, 2851 |
| BmiI | GGNNCC | 2 cut(s) | 2223, 2340 |
| BmrFI | CCNGG | 7 cut(s) | 1055, 1267, 1498, 1596, 1701, 1953, 2070 |
| BmrI | ACTGGG | 1 cut(s) | 2793 |
| BmsI | GCATC | 5 cut(s) | 126, 227, 1534, 1645, 2958 |
| BmuI | ACTGGG | 1 cut(s) | 2793 |
| BoxI | GACNNNNGTC | 1 cut(s) | 29 |
| BpiI | GAAGAC | 3 cut(s) | 29, 1352, 1612 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2654, 2686 |
| BpmI | CTGGAG | 3 cut(s) | 410, 1037, 1722 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1797 |
| BpuEI | CTTGAG | 3 cut(s) | 594, 1260, 2654 |
| BpuMI | CCSGG | 1 cut(s) | 1498 |
| Bsa29I | ATCGAT | 1 cut(s) | 1926 |
| BsaAI | YACGTR | 1 cut(s) | 1687 |
| BsaHI | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| BsaI | GGTCTC | 1 cut(s) | 37 |
| BsaWI | WCCGGW | 2 cut(s) | 699, 1148 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 874, 904 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1988, 2804, 2805, 2938 |
| Bse118I | RCCGGY | 3 cut(s) | 118, 122, 1148 |
| Bse1I | ACTGG | 5 cut(s) | 393, 1729, 2068, 2428, 2799 |
| Bse3DI | GCAATG | 7 cut(s) | 658, 1257, 1491, 1902, 2000, 2506, 2519 |
| BseBI | CCWGG | 6 cut(s) | 1055, 1267, 1596, 1701, 1953, 2070 |
| BseCI | ATCGAT | 1 cut(s) | 1926 |
| BseGI | GGATG | 7 cut(s) | 685, 787, 1660, 1998, 2125, 2814, 2947 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1988, 2804, 2805, 2938 |
| BseMI | GCAATG | 7 cut(s) | 658, 1257, 1491, 1902, 2000, 2506, 2519 |
| BseMII | CTCAG | 6 cut(s) | 1332, 1539, 1788, 2085, 2316, 2400 |
| BseNI | ACTGG | 5 cut(s) | 393, 1729, 2068, 2428, 2799 |
| BseRI | GAGGAG | 3 cut(s) | 1073, 1999, 2405 |
| BseSI | GKGCMC | 3 cut(s) | 808, 1229, 2281 |
| BseX3I | CGGCCG | 1 cut(s) | 120 |
| BseXI | GCAGC | 7 cut(s) | 431, 1249, 1259, 1670, 1827, 2190, 2395 |
| BsgI | GTGCAG | 1 cut(s) | 2189 |
| Bsh1236I | CGCG | 2 cut(s) | 1237, 1242 |
| Bsh1285I | CGRYCG | 2 cut(s) | 123, 2361 |
| BshFI | GGCC | 4 cut(s) | 122, 1143, 1847, 2067 |
| BshTI | ACCGGT | 1 cut(s) | 1148 |
| BshVI | ATCGAT | 1 cut(s) | 1926 |
| BsiEI | CGRYCG | 2 cut(s) | 123, 2361 |
| BsiHKAI | GWGCWC | 4 cut(s) | 808, 1229, 2387, 2667 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2973 |
| BsiSI | CCGG | 5 cut(s) | 119, 123, 700, 1149, 1498 |
| BslFI | GGGAC | 3 cut(s) | 1577, 1797, 1798 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1988, 2804, 2805, 2938 |
| BsmAI | GTCTC | 2 cut(s) | 37, 2543 |
| BsmFI | GGGAC | 3 cut(s) | 1577, 1797, 1798 |
| BsmI | GAATGC | 1 cut(s) | 478 |
| BsnI | GGCC | 4 cut(s) | 122, 1143, 1847, 2067 |
| Bso31I | GGTCTC | 1 cut(s) | 37 |
| BsoBI | CYCGRG | 1 cut(s) | 2973 |
| Bsp1286I | GDGCHC | 5 cut(s) | 808, 1229, 2281, 2387, 2667 |
| Bsp1407I | TGTACA | 1 cut(s) | 1080 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1797 |
| Bsp19I | CCATGG | 1 cut(s) | 1041 |
| BspACI | CCGC | 5 cut(s) | 1235, 1237, 2045, 2638, 2921 |
| BspANI | GGCC | 4 cut(s) | 122, 1143, 1847, 2067 |
| BspCNI | CTCAG | 6 cut(s) | 1333, 1540, 1789, 2086, 2317, 2399 |
| BspDI | ATCGAT | 1 cut(s) | 1926 |
| BspFNI | CGCG | 2 cut(s) | 1237, 1242 |
| BspHI | TCATGA | 3 cut(s) | 481, 1606, 2842 |
| BspLI | GGNNCC | 2 cut(s) | 2223, 2340 |
| BspMAI | CTGCAG | 1 cut(s) | 1845 |
| BspMI | ACCTGC | 2 cut(s) | 808, 2239 |
| BspPI | GGATC | 7 cut(s) | 840, 1940, 2198, 2216, 2229, 2333, 2346 |
| BspQI | GCTCTTC | 2 cut(s) | 1060, 2174 |
| BspTNI | GGTCTC | 1 cut(s) | 37 |
| BsrBI | CCGCTC | 1 cut(s) | 2638 |
| BsrDI | GCAATG | 7 cut(s) | 658, 1257, 1491, 1902, 2000, 2506, 2519 |
| BsrFI | RCCGGY | 3 cut(s) | 118, 122, 1148 |
| BsrGI | TGTACA | 1 cut(s) | 1080 |
| BsrI | ACTGG | 5 cut(s) | 393, 1729, 2068, 2428, 2799 |
| BssAI | RCCGGY | 3 cut(s) | 118, 122, 1148 |
| BssNI | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| BssSI | CACGAG | 1 cut(s) | 1445 |
| BssT1I | CCWWGG | 3 cut(s) | 248, 1041, 2185 |
| Bst2BI | CACGAG | 1 cut(s) | 1445 |
| Bst2UI | CCWGG | 6 cut(s) | 1055, 1267, 1596, 1701, 1953, 2070 |
| Bst4CI | ACNGT | 5 cut(s) | 1179, 1351, 2262, 2767, 2785 |
| Bst6I | CTCTTC | 5 cut(s) | 312, 354, 743, 1060, 2174 |
| BstACI | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| BstAUI | TGTACA | 1 cut(s) | 1080 |
| BstBAI | YACGTR | 1 cut(s) | 1687 |
| BstC8I | GCNNGC | 6 cut(s) | 120, 124, 497, 1414, 1845, 2988 |
| BstDEI | CTNAG | 8 cut(s) | 1341, 1548, 1797, 2094, 2254, 2325, 2386, 2862 |
| BstDSI | CCRYGG | 2 cut(s) | 1041, 1235 |
| BstF5I | GGATG | 7 cut(s) | 685, 787, 1660, 1998, 2125, 2814, 2947 |
| BstFNI | CGCG | 2 cut(s) | 1237, 1242 |
| BstHHI | GCGC | 3 cut(s) | 1092, 1242, 2992 |
| BstMAI | GTCTC | 2 cut(s) | 37, 2543 |
| BstMCI | CGRYCG | 2 cut(s) | 123, 2361 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 182, 1037, 1640, 1837, 2276, 2363, 2474, 2638 |
| BstNI | CCWGG | 6 cut(s) | 1055, 1267, 1596, 1701, 1953, 2070 |
| BstNSI | RCATGY | 2 cut(s) | 812, 1378 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 29 |
| BstSCI | CCNGG | 7 cut(s) | 1053, 1265, 1496, 1594, 1699, 1951, 2068 |
| BstSFI | CTRYAG | 4 cut(s) | 1417, 1841, 2080, 2679 |
| BstSLI | GKGCMC | 3 cut(s) | 808, 1229, 2281 |
| BstUI | CGCG | 2 cut(s) | 1237, 1242 |
| BstV1I | GCAGC | 7 cut(s) | 431, 1249, 1259, 1670, 1827, 2190, 2395 |
| BstV2I | GAAGAC | 3 cut(s) | 29, 1352, 1612 |
| BstX2I | RGATCY | 2 cut(s) | 2221, 2338 |
| BstYI | RGATCY | 2 cut(s) | 2221, 2338 |
| BstZI | CGGCCG | 1 cut(s) | 120 |
| Bsu15I | ATCGAT | 1 cut(s) | 1926 |
| BsuRI | GGCC | 4 cut(s) | 122, 1143, 1847, 2067 |
| BsuTUI | ATCGAT | 1 cut(s) | 1926 |
| BtgI | CCRYGG | 2 cut(s) | 1041, 1235 |
| BtgZI | GCGATG | 2 cut(s) | 76, 300 |
| BtrI | CACGTC | 1 cut(s) | 27 |
| BtsCI | GGATG | 7 cut(s) | 685, 787, 1660, 1998, 2125, 2814, 2947 |
| BtsIMutI | CAGTG | 4 cut(s) | 1564, 2240, 2421, 2772 |
| BveI | ACCTGC | 2 cut(s) | 808, 2239 |
| Cac8I | GCNNGC | 6 cut(s) | 120, 124, 497, 1414, 1845, 2988 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1183, 1643 |
| CciI | TCATGA | 3 cut(s) | 481, 1606, 2842 |
| CfoI | GCGC | 3 cut(s) | 1092, 1242, 2992 |
| Cfr10I | RCCGGY | 3 cut(s) | 118, 122, 1148 |
| Cfr13I | GGNCC | 3 cut(s) | 156, 2066, 2851 |
| Cfr42I | CCGCGG | 1 cut(s) | 1238 |
| ClaI | ATCGAT | 1 cut(s) | 1926 |
| CseI | GACGC | 3 cut(s) | 165, 753, 2016 |
| Csp6I | GTAC | 5 cut(s) | 1081, 1198, 1360, 1671, 2396 |
| CspAI | ACCGGT | 1 cut(s) | 1148 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1885, 1920 |
| CviQI | GTAC | 5 cut(s) | 1081, 1198, 1360, 1671, 2396 |
| DdeI | CTNAG | 8 cut(s) | 1341, 1548, 1797, 2094, 2254, 2325, 2386, 2862 |
| DraI | TTTAAA | 2 cut(s) | 402, 2107 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2459 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1776 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2459 |
| EaeI | YGGCCR | 2 cut(s) | 120, 1141 |
| EagI | CGGCCG | 1 cut(s) | 120 |
| Eam1104I | CTCTTC | 5 cut(s) | 312, 354, 743, 1060, 2174 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1776 |
| EarI | CTCTTC | 5 cut(s) | 312, 354, 743, 1060, 2174 |
| Ecl136II | GAGCTC | 1 cut(s) | 2665 |
| EclXI | CGGCCG | 1 cut(s) | 120 |
| Eco130I | CCWWGG | 3 cut(s) | 248, 1041, 2185 |
| Eco24I | GRGCYC | 1 cut(s) | 2667 |
| Eco31I | GGTCTC | 1 cut(s) | 37 |
| Eco32I | GATATC | 1 cut(s) | 2620 |
| Eco47I | GGWCC | 2 cut(s) | 156, 2851 |
| Eco52I | CGGCCG | 1 cut(s) | 120 |
| Eco53kI | GAGCTC | 1 cut(s) | 2665 |
| Eco57I | CTGAAG | 5 cut(s) | 41, 369, 1890, 2256, 2352 |
| Eco72I | CACGTG | 1 cut(s) | 1687 |
| Eco88I | CYCGRG | 1 cut(s) | 2973 |
| EcoICRI | GAGCTC | 1 cut(s) | 2665 |
| EcoRII | CCWGG | 6 cut(s) | 1053, 1265, 1594, 1699, 1951, 2068 |
| EcoRV | GATATC | 1 cut(s) | 2620 |
| EcoT14I | CCWWGG | 3 cut(s) | 248, 1041, 2185 |
| EcoT38I | GRGCYC | 1 cut(s) | 2667 |
| ErhI | CCWWGG | 3 cut(s) | 248, 1041, 2185 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 325, 357, 1875, 1907 |
| FaqI | GGGAC | 3 cut(s) | 1577, 1797, 1798 |
| FauI | CCCGC | 1 cut(s) | 2038 |
| FauNDI | CATATG | 1 cut(s) | 647 |
| FbaI | TGATCA | 2 cut(s) | 478, 2626 |
| FblI | GTMKAC | 2 cut(s) | 696, 2314 |
| FokI | GGATG | 7 cut(s) | 692, 794, 1667, 2005, 2132, 2821, 2954 |
| FriOI | GRGCYC | 1 cut(s) | 2667 |
| FspBI | CTAG | 8 cut(s) | 140, 249, 1530, 1781, 1833, 1878, 2273, 2736 |
| GlaI | GCGC | 3 cut(s) | 1091, 1241, 2991 |
| GsuI | CTGGAG | 3 cut(s) | 410, 1037, 1722 |
| HaeIII | GGCC | 4 cut(s) | 122, 1143, 1847, 2067 |
| HapII | CCGG | 5 cut(s) | 119, 123, 700, 1149, 1498 |
| HgaI | GACGC | 3 cut(s) | 165, 753, 2016 |
| HhaI | GCGC | 3 cut(s) | 1092, 1242, 2992 |
| Hin1I | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| Hin6I | GCGC | 3 cut(s) | 1090, 1240, 2990 |
| HinP1I | GCGC | 3 cut(s) | 1090, 1240, 2990 |
| HindIII | AAGCTT | 2 cut(s) | 1292, 1601 |
| HinfI | GANTC | 5 cut(s) | 146, 703, 1571, 1997, 2951 |
| HpaII | CCGG | 5 cut(s) | 119, 123, 700, 1149, 1498 |
| HphI | GGTGA | 9 cut(s) | 55, 340, 827, 902, 1495, 2306, 2659, 2701, 2848 |
| Hpy166II | GTNNAC | 8 cut(s) | 697, 806, 1200, 1227, 1435, 1673, 2315, 2533 |
| Hpy8I | GTNNAC | 8 cut(s) | 697, 806, 1200, 1227, 1435, 1673, 2315, 2533 |
| Hpy99I | CGWCG | 4 cut(s) | 181, 264, 469, 769 |
| HpyAV | CCTTC | 9 cut(s) | 318, 509, 790, 881, 1480, 1865, 2443, 2695, 2975 |
| HpyCH4III | ACNGT | 5 cut(s) | 1179, 1351, 2262, 2767, 2785 |
| HpyCH4IV | ACGT | 3 cut(s) | 26, 1686, 2493 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 182, 1037, 1640, 1837, 2276, 2363, 2474, 2638 |
| HpyF3I | CTNAG | 8 cut(s) | 1341, 1548, 1797, 2094, 2254, 2325, 2386, 2862 |
| HpySE526I | ACGT | 3 cut(s) | 26, 1686, 2493 |
| Hsp92I | GRCGYC | 3 cut(s) | 176, 764, 2027 |
| HspAI | GCGC | 3 cut(s) | 1090, 1240, 2990 |
| KroI | GCCGGC | 2 cut(s) | 118, 122 |
| KroNI | GCCGGC | 2 cut(s) | 120, 124 |
| Ksp22I | TGATCA | 2 cut(s) | 478, 2626 |
| KspI | CCGCGG | 1 cut(s) | 1238 |
| LguI | GCTCTTC | 2 cut(s) | 1060, 2174 |
| LmnI | GCTCC | 6 cut(s) | 382, 1056, 1582, 2598, 2753, 2923 |
| Lsp1109I | GCAGC | 7 cut(s) | 431, 1249, 1259, 1670, 1827, 2190, 2395 |
| LweI | GCATC | 5 cut(s) | 126, 227, 1534, 1645, 2958 |
| MaeI | CTAG | 8 cut(s) | 140, 249, 1530, 1781, 1833, 1878, 2273, 2736 |
| MaeII | ACGT | 3 cut(s) | 26, 1686, 2493 |
| MaeIII | GTNAC | 6 cut(s) | 97, 1310, 1687, 2008, 2573, 2717 |
| MbiI | CCGCTC | 1 cut(s) | 2638 |
| MfeI | CAATTG | 1 cut(s) | 1662 |
| MflI | RGATCY | 2 cut(s) | 2221, 2338 |
| MhlI | GDGCHC | 5 cut(s) | 808, 1229, 2281, 2387, 2667 |
| MlsI | TGGCCA | 1 cut(s) | 1143 |
| MluNI | TGGCCA | 1 cut(s) | 1143 |
| MlyI | GAGTC | 1 cut(s) | 2006 |
| MmeI | TCCRAC | 3 cut(s) | 60, 2097, 2721 |
| Mox20I | TGGCCA | 1 cut(s) | 1143 |
| MroNI | GCCGGC | 2 cut(s) | 118, 122 |
| MroXI | GAANNNNTTC | 2 cut(s) | 505, 587 |
| MscI | TGGCCA | 1 cut(s) | 1143 |
| MseI | TTAA | 4 cut(s) | 401, 738, 960, 2106 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2569 |
| Msp20I | TGGCCA | 1 cut(s) | 1143 |
| MspA1I | CMGCKG | 1 cut(s) | 1237 |
| MspI | CCGG | 5 cut(s) | 119, 123, 700, 1149, 1498 |
| MspR9I | CCNGG | 7 cut(s) | 1055, 1267, 1498, 1596, 1701, 1953, 2070 |
| MunI | CAATTG | 1 cut(s) | 1662 |
| Mva1269I | GAATGC | 1 cut(s) | 478 |
| MvaI | CCWGG | 6 cut(s) | 1055, 1267, 1596, 1701, 1953, 2070 |
| MvnI | CGCG | 2 cut(s) | 1237, 1242 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 182, 1037, 1640, 1837, 2276, 2363, 2474, 2638 |
| NaeI | GCCGGC | 2 cut(s) | 120, 124 |
| NciI | CCSGG | 1 cut(s) | 1498 |
| NcoI | CCATGG | 1 cut(s) | 1041 |
| NdeI | CATATG | 1 cut(s) | 647 |
| NgoMIV | GCCGGC | 2 cut(s) | 118, 122 |
| NlaIV | GGNNCC | 2 cut(s) | 2223, 2340 |
| NmuCI | GTSAC | 2 cut(s) | 1687, 2008 |
| NspI | RCATGY | 2 cut(s) | 812, 1378 |
| OliI | CACNNNNGTG | 1 cut(s) | 2569 |
| PagI | TCATGA | 3 cut(s) | 481, 1606, 2842 |
| PaqCI | CACCTGC | 1 cut(s) | 2239 |
| PciSI | GCTCTTC | 2 cut(s) | 1060, 2174 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1443 |
| PctI | GAATGC | 1 cut(s) | 478 |
| PdiI | GCCGGC | 2 cut(s) | 120, 124 |
| PdmI | GAANNNNTTC | 2 cut(s) | 505, 587 |
| PfeI | GAWTC | 4 cut(s) | 146, 703, 1571, 2951 |
| PfoI | TCCNGGA | 1 cut(s) | 1053 |
| PinAI | ACCGGT | 1 cut(s) | 1148 |
| Ple19I | CGATCG | 1 cut(s) | 2361 |
| PleI | GAGTC | 1 cut(s) | 2005 |
| PmaCI | CACGTG | 1 cut(s) | 1687 |
| PmlI | CACGTG | 1 cut(s) | 1687 |
| PpsI | GAGTC | 1 cut(s) | 2005 |
| Ppu21I | YACGTR | 1 cut(s) | 1687 |
| PshAI | GACNNNNGTC | 1 cut(s) | 29 |
| Psp124BI | GAGCTC | 1 cut(s) | 2667 |
| Psp6I | CCWGG | 6 cut(s) | 1053, 1265, 1594, 1699, 1951, 2068 |
| PspCI | CACGTG | 1 cut(s) | 1687 |
| PspGI | CCWGG | 6 cut(s) | 1053, 1265, 1594, 1699, 1951, 2068 |
| PspN4I | GGNNCC | 2 cut(s) | 2223, 2340 |
| PspPI | GGNCC | 3 cut(s) | 156, 2066, 2851 |
| PstI | CTGCAG | 1 cut(s) | 1845 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1183, 1643 |
| PsuI | RGATCY | 2 cut(s) | 2221, 2338 |
| PvuI | CGATCG | 1 cut(s) | 2361 |
| RsaI | GTAC | 5 cut(s) | 1082, 1199, 1361, 1672, 2397 |
| RsaNI | GTAC | 5 cut(s) | 1081, 1198, 1360, 1671, 2396 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2569 |
| SacI | GAGCTC | 1 cut(s) | 2667 |
| SacII | CCGCGG | 1 cut(s) | 1238 |
| SapI | GCTCTTC | 2 cut(s) | 1060, 2174 |
| SaqAI | TTAA | 4 cut(s) | 401, 738, 960, 2106 |
| Sau96I | GGNCC | 3 cut(s) | 156, 2066, 2851 |
| SchI | GAGTC | 1 cut(s) | 2006 |
| ScrFI | CCNGG | 7 cut(s) | 1055, 1267, 1498, 1596, 1701, 1953, 2070 |
| SduI | GDGCHC | 5 cut(s) | 808, 1229, 2281, 2387, 2667 |
| SfaNI | GCATC | 5 cut(s) | 126, 227, 1534, 1645, 2958 |
| SfcI | CTRYAG | 4 cut(s) | 1417, 1841, 2080, 2679 |
| Sfr303I | CCGCGG | 1 cut(s) | 1238 |
| SgrBI | CCGCGG | 1 cut(s) | 1238 |
| SinI | GGWCC | 2 cut(s) | 156, 2851 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2569 |
| SmlI | CTYRAG | 3 cut(s) | 609, 1275, 2633 |
| SmoI | CTYRAG | 3 cut(s) | 609, 1275, 2633 |
| SsiI | CCGC | 5 cut(s) | 1235, 1237, 2045, 2638, 2921 |
| SspI | AATATT | 2 cut(s) | 546, 1758 |
| SspMI | CTAG | 8 cut(s) | 140, 249, 1530, 1781, 1833, 1878, 2273, 2736 |
| SstI | GAGCTC | 1 cut(s) | 2667 |
| StyD4I | CCNGG | 7 cut(s) | 1053, 1265, 1496, 1594, 1699, 1951, 2068 |
| StyI | CCWWGG | 3 cut(s) | 248, 1041, 2185 |
| TaaI | ACNGT | 5 cut(s) | 1179, 1351, 2262, 2767, 2785 |
| TaiI | ACGT | 3 cut(s) | 29, 1689, 2496 |
| TatI | WGTACW | 3 cut(s) | 1080, 1359, 1670 |
| TauI | GCSGC | 3 cut(s) | 1237, 1240, 2641 |
| TfiI | GAWTC | 4 cut(s) | 146, 703, 1571, 2951 |
| Tru1I | TTAA | 4 cut(s) | 401, 738, 960, 2106 |
| Tru9I | TTAA | 4 cut(s) | 401, 738, 960, 2106 |
| TscAI | CASTG | 4 cut(s) | 1564, 2247, 2428, 2772 |
| TseFI | GTSAC | 2 cut(s) | 1687, 2008 |
| TseI | GCWGC | 7 cut(s) | 419, 1247, 1262, 1658, 1840, 2203, 2408 |
| Tsp45I | GTSAC | 2 cut(s) | 1687, 2008 |
| TspDTI | ATGAA | 9 cut(s) | 306, 539, 612, 684, 1595, 1763, 1767, 1982, 2618 |
| TspGWI | ACGGA | 2 cut(s) | 248, 2612 |
| TspRI | CASTG | 4 cut(s) | 1564, 2247, 2428, 2772 |
| VneI | GTGCAC | 2 cut(s) | 804, 1225 |
| VpaK11BI | GGWCC | 2 cut(s) | 156, 2851 |
| XapI | RAATTY | 3 cut(s) | 397, 1553, 2485 |
| XceI | RCATGY | 2 cut(s) | 812, 1378 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2806 |
| XmaJI | CCTAGG | 1 cut(s) | 248 |
| XmiI | GTMKAC | 2 cut(s) | 696, 2314 |
| XmnI | GAANNNNTTC | 2 cut(s) | 505, 587 |
| XspI | CTAG | 8 cut(s) | 140, 249, 1530, 1781, 1833, 1878, 2273, 2736 |