Pre-mRNA-splicing factor ATP-dependent RNA helicase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Helicase_C | PF00271 | 609 - 735 | 9.6e-12 | Helicase conserved C-terminal domain |
| WHD_HA2 | PF04408 | 797 - 825 | 9.1e-10 | Helicase associated domain (HA2), winged-helix |
| HA2_C | PF21010 | 826 - 887 | 4.2e-11 | Helicase associated domain (HA2), ratchet-like |
| OB_NTP_bind | PF07717 | 890 - 926 | 8.4e-11 | Oligonucleotide/oligosaccharide-binding (OB)-fold |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2597 |
| Acc36I | ACCTGC | 1 cut(s) | 2174 |
| Acc65I | GGTACC | 1 cut(s) | 2269 |
| AccB1I | GGYRCC | 1 cut(s) | 2269 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2126, 2698 |
| AccBSI | CCGCTC | 1 cut(s) | 535 |
| AccI | GTMKAC | 1 cut(s) | 2452 |
| AccII | CGCG | 1 cut(s) | 2033 |
| AccIII | TCCGGA | 1 cut(s) | 221 |
| AciI | CCGC | 8 cut(s) | 457, 535, 844, 1151, 1385, 1886, 2363, 2771 |
| AclWI | GGATC | 3 cut(s) | 1014, 2078, 2471 |
| AcoI | YGGCCR | 3 cut(s) | 936, 2169, 2460 |
| AcsI | RAATTY | 5 cut(s) | 179, 1526, 1728, 1989, 2623 |
| AcuI | CTGAAG | 5 cut(s) | 207, 633, 687, 2028, 2762 |
| AcvI | CACGTG | 2 cut(s) | 176, 775 |
| AdeI | CACNNNGTG | 1 cut(s) | 2414 |
| AfaI | GTAC | 5 cut(s) | 86, 438, 875, 2271, 2516 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 964, 1135, 1296, 2126, 2178, 2587, 2698, 2788 |
| AflIII | ACRYGT | 1 cut(s) | 173 |
| AgeI | ACCGGT | 1 cut(s) | 1289 |
| AhlI | ACTAGT | 1 cut(s) | 679 |
| AjiI | CACGTC | 1 cut(s) | 27 |
| AjnI | CCWGG | 6 cut(s) | 359, 441, 957, 1194, 1837, 2089 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1166, 1198 |
| Alw21I | GWGCWC | 3 cut(s) | 774, 1367, 2310 |
| Alw26I | GTCTC | 2 cut(s) | 120, 2719 |
| Alw44I | GTGCAC | 1 cut(s) | 1363 |
| AlwI | GGATC | 3 cut(s) | 1014, 2078, 2471 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1781 |
| Ama87I | CYCGRG | 1 cut(s) | 403 |
| Aor13HI | TCCGGA | 1 cut(s) | 221 |
| AoxI | GGCC | 6 cut(s) | 399, 936, 1332, 1578, 2169, 2460 |
| ApaLI | GTGCAC | 1 cut(s) | 1363 |
| ApeKI | GCWGC | 4 cut(s) | 257, 575, 1400, 1796 |
| ApoI | RAATTY | 5 cut(s) | 179, 1526, 1728, 1989, 2623 |
| AsiGI | ACCGGT | 1 cut(s) | 1289 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 386, 1594 |
| Asp718I | GGTACC | 1 cut(s) | 2269 |
| AspLEI | GCGC | 1 cut(s) | 552 |
| AspS9I | GGNCC | 7 cut(s) | 156, 224, 955, 1087, 1579, 2204, 2359 |
| AsuC2I | CCSGG | 2 cut(s) | 1734, 2208 |
| AsuHPI | GGTGA | 8 cut(s) | 280, 338, 442, 1001, 1136, 1295, 1547, 2444 |
| AvaI | CYCGRG | 1 cut(s) | 403 |
| AvaII | GGWCC | 6 cut(s) | 156, 224, 955, 1087, 2204, 2359 |
| BaeGI | GKGCMC | 2 cut(s) | 210, 1367 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1304, 1337 |
| BalI | TGGCCA | 1 cut(s) | 938 |
| BanI | GGYRCC | 1 cut(s) | 2269 |
| BanII | GRGCYC | 2 cut(s) | 2001, 2838 |
| BauI | CACGAG | 2 cut(s) | 1328, 2823 |
| BbrPI | CACGTG | 2 cut(s) | 176, 775 |
| BbsI | GAAGAC | 4 cut(s) | 29, 287, 318, 327 |
| Bbv12I | GWGCWC | 3 cut(s) | 774, 1367, 2310 |
| BbvI | GCAGC | 4 cut(s) | 244, 587, 1387, 1808 |
| BciT130I | CCWGG | 6 cut(s) | 361, 443, 959, 1196, 1839, 2091 |
| BciVI | GTATCC | 2 cut(s) | 1331, 2339 |
| BclI | TGATCA | 1 cut(s) | 523 |
| BcnI | CCSGG | 2 cut(s) | 1734, 2208 |
| BcoDI | GTCTC | 2 cut(s) | 120, 2719 |
| BcuI | ACTAGT | 1 cut(s) | 679 |
| BfaI | CTAG | 1 cut(s) | 680 |
| BfmI | CTRYAG | 3 cut(s) | 949, 1031, 1555 |
| BfuAI | ACCTGC | 1 cut(s) | 2174 |
| BfuI | GTATCC | 2 cut(s) | 1331, 2339 |
| BglII | AGATCT | 1 cut(s) | 1543 |
| BisI | GCNGC | 5 cut(s) | 258, 576, 1386, 1401, 1797 |
| BlpI | GCTNAGC | 1 cut(s) | 1935 |
| BlsI | GCNGC | 5 cut(s) | 259, 577, 1387, 1402, 1798 |
| Bme1390I | CCNGG | 8 cut(s) | 361, 443, 959, 1196, 1734, 1839, 2091, 2208 |
| Bme18I | GGWCC | 6 cut(s) | 156, 224, 955, 1087, 2204, 2359 |
| BmeT110I | CYCGRG | 1 cut(s) | 403 |
| BmgBI | CACGTC | 1 cut(s) | 27 |
| BmgT120I | GGNCC | 7 cut(s) | 156, 224, 955, 1087, 1579, 2204, 2359 |
| BmiI | GGNNCC | 5 cut(s) | 956, 2205, 2206, 2271, 2361 |
| BmrFI | CCNGG | 8 cut(s) | 361, 443, 959, 1196, 1734, 1839, 2091, 2208 |
| BmrI | ACTGGG | 3 cut(s) | 1312, 2136, 2643 |
| BmsI | GCATC | 6 cut(s) | 289, 877, 1118, 1672, 1783, 2174 |
| BmuI | ACTGGG | 3 cut(s) | 1312, 2136, 2643 |
| BpiI | GAAGAC | 4 cut(s) | 29, 287, 318, 327 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 2820 |
| BpmI | CTGGAG | 5 cut(s) | 102, 184, 746, 1178, 1860 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1918 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1935 |
| BpuEI | CTTGAG | 5 cut(s) | 848, 1181, 1398, 1651, 2648 |
| BpuMI | CCSGG | 2 cut(s) | 1734, 2208 |
| BsaAI | YACGTR | 2 cut(s) | 176, 775 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1068, 1260 |
| BsaJI | CCNNGG | 8 cut(s) | 1531, 1733, 2089, 2090, 2120, 2323, 2391, 2712 |
| BsaWI | WCCGGW | 2 cut(s) | 221, 1289 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2188, 2218 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 964, 1135, 1296, 2126, 2178, 2587, 2698, 2788 |
| Bse118I | RCCGGY | 4 cut(s) | 70, 1147, 1289, 2220 |
| Bse1I | ACTGG | 6 cut(s) | 344, 729, 1129, 1318, 2131, 2638 |
| Bse3DI | GCAATG | 3 cut(s) | 267, 2541, 2657 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1068, 1260 |
| BseAI | TCCGGA | 1 cut(s) | 221 |
| BseBI | CCWGG | 6 cut(s) | 361, 443, 959, 1196, 1839, 2091 |
| BseDI | CCNNGG | 8 cut(s) | 1531, 1733, 2089, 2090, 2120, 2323, 2391, 2712 |
| BseJI | GATNNNNATC | 2 cut(s) | 1068, 1260 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 964, 1135, 1296, 2126, 2178, 2587, 2698, 2788 |
| BseMI | GCAATG | 3 cut(s) | 267, 2541, 2657 |
| BseMII | CTCAG | 6 cut(s) | 80, 345, 1677, 1926, 1965, 2241 |
| BseNI | ACTGG | 6 cut(s) | 344, 729, 1129, 1318, 2131, 2638 |
| BseRI | GAGGAG | 2 cut(s) | 151, 638 |
| BseSI | GKGCMC | 2 cut(s) | 210, 1367 |
| BseXI | GCAGC | 4 cut(s) | 244, 587, 1387, 1808 |
| BseYI | CCCAGC | 1 cut(s) | 962 |
| BsgI | GTGCAG | 1 cut(s) | 1794 |
| Bsh1236I | CGCG | 1 cut(s) | 2033 |
| BshFI | GGCC | 6 cut(s) | 401, 938, 1334, 1580, 2171, 2462 |
| BshNI | GGYRCC | 1 cut(s) | 2269 |
| BshTI | ACCGGT | 1 cut(s) | 1289 |
| BsiHKAI | GWGCWC | 3 cut(s) | 774, 1367, 2310 |
| BsiHKCI | CYCGRG | 1 cut(s) | 403 |
| BsiSI | CCGG | 8 cut(s) | 71, 222, 1148, 1290, 1335, 1733, 2208, 2221 |
| BslFI | GGGAC | 5 cut(s) | 581, 968, 1935, 1936, 2190 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 964, 1135, 1296, 2126, 2178, 2587, 2698, 2788 |
| BsmAI | GTCTC | 2 cut(s) | 120, 2719 |
| BsmFI | GGGAC | 5 cut(s) | 581, 968, 1935, 1936, 2190 |
| BsnI | GGCC | 6 cut(s) | 401, 938, 1334, 1580, 2171, 2462 |
| BsoBI | CYCGRG | 1 cut(s) | 403 |
| Bsp1286I | GDGCHC | 6 cut(s) | 210, 774, 1367, 2001, 2310, 2838 |
| Bsp13I | TCCGGA | 1 cut(s) | 221 |
| Bsp1407I | TGTACA | 1 cut(s) | 436 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1935 |
| Bsp68I | TCGCGA | 1 cut(s) | 2033 |
| BspACI | CCGC | 8 cut(s) | 457, 535, 844, 1151, 1385, 1886, 2363, 2771 |
| BspANI | GGCC | 6 cut(s) | 401, 938, 1334, 1580, 2171, 2462 |
| BspCNI | CTCAG | 6 cut(s) | 79, 346, 1678, 1927, 1966, 2242 |
| BspEI | TCCGGA | 1 cut(s) | 221 |
| BspFNI | CGCG | 1 cut(s) | 2033 |
| BspLI | GGNNCC | 5 cut(s) | 956, 2205, 2206, 2271, 2361 |
| BspMAI | CTGCAG | 1 cut(s) | 1035 |
| BspMI | ACCTGC | 1 cut(s) | 2174 |
| BspPI | GGATC | 3 cut(s) | 1014, 2078, 2471 |
| BspT107I | GGYRCC | 1 cut(s) | 2269 |
| BsrBI | CCGCTC | 1 cut(s) | 535 |
| BsrDI | GCAATG | 3 cut(s) | 267, 2541, 2657 |
| BsrFI | RCCGGY | 4 cut(s) | 70, 1147, 1289, 2220 |
| BsrGI | TGTACA | 1 cut(s) | 436 |
| BsrI | ACTGG | 6 cut(s) | 344, 729, 1129, 1318, 2131, 2638 |
| BssAI | RCCGGY | 4 cut(s) | 70, 1147, 1289, 2220 |
| BssECI | CCNNGG | 8 cut(s) | 1531, 1733, 2089, 2090, 2120, 2323, 2391, 2712 |
| BssSI | CACGAG | 2 cut(s) | 1328, 2823 |
| BssT1I | CCWWGG | 5 cut(s) | 1531, 2120, 2323, 2391, 2712 |
| Bst2BI | CACGAG | 2 cut(s) | 1328, 2823 |
| Bst2UI | CCWGG | 6 cut(s) | 361, 443, 959, 1196, 1839, 2091 |
| Bst4CI | ACNGT | 7 cut(s) | 387, 1489, 1559, 2269, 2400, 2582, 2619 |
| Bst6I | CTCTTC | 2 cut(s) | 615, 917 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2414 |
| BstAUI | TGTACA | 1 cut(s) | 436 |
| BstBAI | YACGTR | 2 cut(s) | 176, 775 |
| BstC8I | GCNNGC | 4 cut(s) | 72, 846, 1552, 2838 |
| BstDEI | CTNAG | 9 cut(s) | 66, 354, 1686, 1918, 1935, 1974, 2250, 2411, 2490 |
| BstEII | GGTNACC | 1 cut(s) | 1124 |
| BstFNI | CGCG | 1 cut(s) | 2033 |
| BstHHI | GCGC | 1 cut(s) | 552 |
| BstMAI | GTCTC | 2 cut(s) | 120, 2719 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 547, 778, 1778, 2414, 2612, 2668 |
| BstNI | CCWGG | 6 cut(s) | 361, 443, 959, 1196, 1839, 2091 |
| BstPI | GGTNACC | 1 cut(s) | 1124 |
| BstSCI | CCNGG | 8 cut(s) | 359, 441, 957, 1194, 1732, 1837, 2089, 2206 |
| BstSFI | CTRYAG | 3 cut(s) | 949, 1031, 1555 |
| BstSLI | GKGCMC | 2 cut(s) | 210, 1367 |
| BstUI | CGCG | 1 cut(s) | 2033 |
| BstV1I | GCAGC | 4 cut(s) | 244, 587, 1387, 1808 |
| BstV2I | GAAGAC | 4 cut(s) | 29, 287, 318, 327 |
| BstX2I | RGATCY | 1 cut(s) | 1543 |
| BstYI | RGATCY | 1 cut(s) | 1543 |
| BsuI | GTATCC | 2 cut(s) | 1331, 2339 |
| BsuRI | GGCC | 6 cut(s) | 401, 938, 1334, 1580, 2171, 2462 |
| BtgZI | GCGATG | 1 cut(s) | 2638 |
| BtrI | CACGTC | 1 cut(s) | 27 |
| BtsI | GCAGTG | 2 cut(s) | 1028, 1811 |
| BtsIMutI | CAGTG | 7 cut(s) | 783, 1028, 1564, 1702, 1811, 2631, 2811 |
| BtuMI | TCGCGA | 1 cut(s) | 2033 |
| BveI | ACCTGC | 1 cut(s) | 2174 |
| Cac8I | GCNNGC | 4 cut(s) | 72, 846, 1552, 2838 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1781 |
| CfoI | GCGC | 1 cut(s) | 552 |
| Cfr10I | RCCGGY | 4 cut(s) | 70, 1147, 1289, 2220 |
| Cfr13I | GGNCC | 7 cut(s) | 156, 224, 955, 1087, 1579, 2204, 2359 |
| CpoI | CGGWCCG | 1 cut(s) | 224 |
| Csp6I | GTAC | 5 cut(s) | 85, 437, 874, 2270, 2515 |
| CspAI | ACCGGT | 1 cut(s) | 1289 |
| CspI | CGGWCCG | 1 cut(s) | 224 |
| CviQI | GTAC | 5 cut(s) | 85, 437, 874, 2270, 2515 |
| DdeI | CTNAG | 9 cut(s) | 66, 354, 1686, 1918, 1935, 1974, 2250, 2411, 2490 |
| DraI | TTTAAA | 1 cut(s) | 702 |
| DraIII | CACNNNGTG | 1 cut(s) | 2414 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2597 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2597 |
| EaeI | YGGCCR | 3 cut(s) | 936, 2169, 2460 |
| Eam1104I | CTCTTC | 2 cut(s) | 615, 917 |
| EarI | CTCTTC | 2 cut(s) | 615, 917 |
| EciI | GGCGGA | 1 cut(s) | 1166 |
| Eco130I | CCWWGG | 5 cut(s) | 1531, 2120, 2323, 2391, 2712 |
| Eco147I | AGGCCT | 1 cut(s) | 401 |
| Eco24I | GRGCYC | 2 cut(s) | 2001, 2838 |
| Eco47I | GGWCC | 6 cut(s) | 156, 224, 955, 1087, 2204, 2359 |
| Eco57I | CTGAAG | 5 cut(s) | 207, 633, 687, 2028, 2762 |
| Eco72I | CACGTG | 2 cut(s) | 176, 775 |
| Eco88I | CYCGRG | 1 cut(s) | 403 |
| Eco91I | GGTNACC | 1 cut(s) | 1124 |
| EcoO109I | RGGNCCY | 2 cut(s) | 955, 2204 |
| EcoO65I | GGTNACC | 1 cut(s) | 1124 |
| EcoRI | GAATTC | 1 cut(s) | 1526 |
| EcoRII | CCWGG | 6 cut(s) | 359, 441, 957, 1194, 1837, 2089 |
| EcoT14I | CCWWGG | 5 cut(s) | 1531, 2120, 2323, 2391, 2712 |
| EcoT38I | GRGCYC | 2 cut(s) | 2001, 2838 |
| ErhI | CCWWGG | 5 cut(s) | 1531, 2120, 2323, 2391, 2712 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 457, 489 |
| FaqI | GGGAC | 5 cut(s) | 581, 968, 1935, 1936, 2190 |
| FauI | CCCGC | 4 cut(s) | 450, 528, 837, 2370 |
| FauNDI | CATATG | 1 cut(s) | 286 |
| FbaI | TGATCA | 1 cut(s) | 523 |
| FblI | GTMKAC | 1 cut(s) | 2452 |
| Fnu4HI | GCNGC | 5 cut(s) | 258, 576, 1386, 1401, 1797 |
| FriOI | GRGCYC | 2 cut(s) | 2001, 2838 |
| Fsp4HI | GCNGC | 5 cut(s) | 258, 576, 1386, 1401, 1797 |
| FspBI | CTAG | 1 cut(s) | 680 |
| GlaI | GCGC | 1 cut(s) | 551 |
| GluI | GCNGC | 5 cut(s) | 258, 576, 1386, 1401, 1797 |
| GsaI | CCCAGC | 1 cut(s) | 966 |
| GsuI | CTGGAG | 5 cut(s) | 102, 184, 746, 1178, 1860 |
| HaeIII | GGCC | 6 cut(s) | 401, 938, 1334, 1580, 2171, 2462 |
| HapII | CCGG | 8 cut(s) | 71, 222, 1148, 1290, 1335, 1733, 2208, 2221 |
| HhaI | GCGC | 1 cut(s) | 552 |
| Hin6I | GCGC | 1 cut(s) | 550 |
| HinP1I | GCGC | 1 cut(s) | 550 |
| HincII | GTYRAC | 2 cut(s) | 591, 2439 |
| HindII | GTYRAC | 2 cut(s) | 591, 2439 |
| HindIII | AAGCTT | 2 cut(s) | 137, 996 |
| HpaI | GTTAAC | 1 cut(s) | 591 |
| HpaII | CCGG | 8 cut(s) | 71, 222, 1148, 1290, 1335, 1733, 2208, 2221 |
| HphI | GGTGA | 8 cut(s) | 280, 338, 442, 1001, 1136, 1295, 1547, 2444 |
| Hpy166II | GTNNAC | 6 cut(s) | 439, 591, 1365, 1538, 2439, 2453 |
| Hpy8I | GTNNAC | 6 cut(s) | 439, 591, 1365, 1538, 2439, 2453 |
| Hpy99I | CGWCG | 3 cut(s) | 320, 801, 1380 |
| HpyAV | CCTTC | 3 cut(s) | 391, 2003, 2825 |
| HpyCH4III | ACNGT | 7 cut(s) | 387, 1489, 1559, 2269, 2400, 2582, 2619 |
| HpyCH4IV | ACGT | 5 cut(s) | 26, 175, 774, 796, 1348 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 547, 778, 1778, 2414, 2612, 2668 |
| HpyF3I | CTNAG | 9 cut(s) | 66, 354, 1686, 1918, 1935, 1974, 2250, 2411, 2490 |
| HpySE526I | ACGT | 5 cut(s) | 26, 175, 774, 796, 1348 |
| HspAI | GCGC | 1 cut(s) | 550 |
| KflI | GGGWCCC | 1 cut(s) | 2204 |
| Kpn2I | TCCGGA | 1 cut(s) | 221 |
| KpnI | GGTACC | 1 cut(s) | 2273 |
| KroI | GCCGGC | 1 cut(s) | 70 |
| KroNI | GCCGGC | 1 cut(s) | 72 |
| Ksp22I | TGATCA | 1 cut(s) | 523 |
| KspAI | GTTAAC | 1 cut(s) | 591 |
| LmnI | GCTCC | 6 cut(s) | 6, 538, 1197, 1720, 2155, 2773 |
| Lsp1109I | GCAGC | 4 cut(s) | 244, 587, 1387, 1808 |
| LweI | GCATC | 6 cut(s) | 289, 877, 1118, 1672, 1783, 2174 |
| MaeI | CTAG | 1 cut(s) | 680 |
| MaeII | ACGT | 5 cut(s) | 26, 175, 774, 796, 1348 |
| MaeIII | GTNAC | 6 cut(s) | 809, 835, 1124, 1804, 1825, 2731 |
| MbiI | CCGCTC | 1 cut(s) | 535 |
| MfeI | CAATTG | 1 cut(s) | 1800 |
| MflI | RGATCY | 1 cut(s) | 1543 |
| MhlI | GDGCHC | 6 cut(s) | 210, 774, 1367, 2001, 2310, 2838 |
| MlsI | TGGCCA | 1 cut(s) | 938 |
| MluNI | TGGCCA | 1 cut(s) | 938 |
| MlyI | GAGTC | 5 cut(s) | 365, 415, 509, 1172, 2144 |
| MmeI | TCCRAC | 1 cut(s) | 1164 |
| Mox20I | TGGCCA | 1 cut(s) | 938 |
| MroI | TCCGGA | 1 cut(s) | 221 |
| MroNI | GCCGGC | 1 cut(s) | 70 |
| MroXI | GAANNNNTTC | 2 cut(s) | 386, 1594 |
| MscI | TGGCCA | 1 cut(s) | 938 |
| MseI | TTAA | 6 cut(s) | 590, 674, 701, 806, 1653, 2420 |
| Msp20I | TGGCCA | 1 cut(s) | 938 |
| MspA1I | CMGCKG | 2 cut(s) | 122, 1886 |
| MspI | CCGG | 8 cut(s) | 71, 222, 1148, 1290, 1335, 1733, 2208, 2221 |
| MspR9I | CCNGG | 8 cut(s) | 361, 443, 959, 1196, 1734, 1839, 2091, 2208 |
| MunI | CAATTG | 1 cut(s) | 1800 |
| MvaI | CCWGG | 6 cut(s) | 361, 443, 959, 1196, 1839, 2091 |
| MvnI | CGCG | 1 cut(s) | 2033 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 547, 778, 1778, 2414, 2612, 2668 |
| NaeI | GCCGGC | 1 cut(s) | 72 |
| NciI | CCSGG | 2 cut(s) | 1734, 2208 |
| NdeI | CATATG | 1 cut(s) | 286 |
| NgoMIV | GCCGGC | 1 cut(s) | 70 |
| NlaIV | GGNNCC | 5 cut(s) | 956, 2205, 2206, 2271, 2361 |
| NmeAIII | GCCGAG | 1 cut(s) | 2488 |
| NmuCI | GTSAC | 2 cut(s) | 1124, 1825 |
| NruI | TCGCGA | 1 cut(s) | 2033 |
| PaeR7I | CTCGAG | 1 cut(s) | 403 |
| PasI | CCCWGGG | 1 cut(s) | 2090 |
| PceI | AGGCCT | 1 cut(s) | 401 |
| PdiI | GCCGGC | 1 cut(s) | 72 |
| PdmI | GAANNNNTTC | 2 cut(s) | 386, 1594 |
| PfeI | GAWTC | 7 cut(s) | 146, 371, 862, 880, 976, 1709, 2801 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2126, 2698 |
| PfoI | TCCNGGA | 3 cut(s) | 359, 1194, 2206 |
| PinAI | ACCGGT | 1 cut(s) | 1289 |
| PkrI | GCNGC | 5 cut(s) | 259, 577, 1387, 1402, 1798 |
| PleI | GAGTC | 5 cut(s) | 364, 414, 508, 1171, 2143 |
| PmaCI | CACGTG | 2 cut(s) | 176, 775 |
| PmlI | CACGTG | 2 cut(s) | 176, 775 |
| PpsI | GAGTC | 5 cut(s) | 364, 414, 508, 1171, 2143 |
| Ppu21I | YACGTR | 2 cut(s) | 176, 775 |
| PpuMI | RGGWCCY | 2 cut(s) | 955, 2204 |
| Psp5II | RGGWCCY | 2 cut(s) | 955, 2204 |
| Psp6I | CCWGG | 6 cut(s) | 359, 441, 957, 1194, 1837, 2089 |
| PspCI | CACGTG | 2 cut(s) | 176, 775 |
| PspEI | GGTNACC | 1 cut(s) | 1124 |
| PspFI | CCCAGC | 1 cut(s) | 962 |
| PspGI | CCWGG | 6 cut(s) | 359, 441, 957, 1194, 1837, 2089 |
| PspN4I | GGNNCC | 5 cut(s) | 956, 2205, 2206, 2271, 2361 |
| PspPI | GGNCC | 7 cut(s) | 156, 224, 955, 1087, 1579, 2204, 2359 |
| PspPPI | RGGWCCY | 2 cut(s) | 955, 2204 |
| PspXI | VCTCGAGB | 1 cut(s) | 403 |
| PstI | CTGCAG | 1 cut(s) | 1035 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1781 |
| PsuI | RGATCY | 1 cut(s) | 1543 |
| PvuII | CAGCTG | 1 cut(s) | 122 |
| RruI | TCGCGA | 1 cut(s) | 2033 |
| RsaI | GTAC | 5 cut(s) | 86, 438, 875, 2271, 2516 |
| RsaNI | GTAC | 5 cut(s) | 85, 437, 874, 2270, 2515 |
| Rsr2I | CGGWCCG | 1 cut(s) | 224 |
| RsrII | CGGWCCG | 1 cut(s) | 224 |
| SaqAI | TTAA | 6 cut(s) | 590, 674, 701, 806, 1653, 2420 |
| SatI | GCNGC | 5 cut(s) | 258, 576, 1386, 1401, 1797 |
| Sau96I | GGNCC | 7 cut(s) | 156, 224, 955, 1087, 1579, 2204, 2359 |
| SchI | GAGTC | 5 cut(s) | 365, 415, 509, 1172, 2144 |
| ScrFI | CCNGG | 8 cut(s) | 361, 443, 959, 1196, 1734, 1839, 2091, 2208 |
| SduI | GDGCHC | 6 cut(s) | 210, 774, 1367, 2001, 2310, 2838 |
| SfaNI | GCATC | 6 cut(s) | 289, 877, 1118, 1672, 1783, 2174 |
| SfcI | CTRYAG | 3 cut(s) | 949, 1031, 1555 |
| Sfr274I | CTCGAG | 1 cut(s) | 403 |
| SinI | GGWCC | 6 cut(s) | 156, 224, 955, 1087, 2204, 2359 |
| SlaI | CTCGAG | 1 cut(s) | 403 |
| SmlI | CTYRAG | 6 cut(s) | 403, 827, 1160, 1413, 1666, 2663 |
| SmoI | CTYRAG | 6 cut(s) | 403, 827, 1160, 1413, 1666, 2663 |
| SpeI | ACTAGT | 1 cut(s) | 679 |
| SseBI | AGGCCT | 1 cut(s) | 401 |
| SsiI | CCGC | 8 cut(s) | 457, 535, 844, 1151, 1385, 1886, 2363, 2771 |
| SspI | AATATT | 3 cut(s) | 698, 1896, 2574 |
| SspMI | CTAG | 1 cut(s) | 680 |
| StuI | AGGCCT | 1 cut(s) | 401 |
| StyD4I | CCNGG | 8 cut(s) | 359, 441, 957, 1194, 1732, 1837, 2089, 2206 |
| StyI | CCWWGG | 5 cut(s) | 1531, 2120, 2323, 2391, 2712 |
| TaaI | ACNGT | 7 cut(s) | 387, 1489, 1559, 2269, 2400, 2582, 2619 |
| TaiI | ACGT | 5 cut(s) | 29, 178, 777, 799, 1351 |
| TaqI | TCGA | 7 cut(s) | 164, 404, 1166, 1464, 1636, 1642, 2188 |
| TaqII | GACCGA | 1 cut(s) | 241 |
| TatI | WGTACW | 4 cut(s) | 84, 436, 873, 2514 |
| TauI | GCSGC | 1 cut(s) | 1388 |
| TfiI | GAWTC | 7 cut(s) | 146, 371, 862, 880, 976, 1709, 2801 |
| Tru1I | TTAA | 6 cut(s) | 590, 674, 701, 806, 1653, 2420 |
| Tru9I | TTAA | 6 cut(s) | 590, 674, 701, 806, 1653, 2420 |
| TscAI | CASTG | 7 cut(s) | 790, 1035, 1564, 1702, 1818, 2638, 2811 |
| TseFI | GTSAC | 2 cut(s) | 1124, 1825 |
| TseI | GCWGC | 4 cut(s) | 257, 575, 1400, 1796 |
| Tsp45I | GTSAC | 2 cut(s) | 1124, 1825 |
| TspDTI | ATGAA | 8 cut(s) | 324, 432, 498, 675, 756, 989, 1090, 2120 |
| TspGWI | ACGGA | 2 cut(s) | 2470, 2750 |
| TspRI | CASTG | 7 cut(s) | 790, 1035, 1564, 1702, 1818, 2638, 2811 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2126, 2698 |
| VneI | GTGCAC | 1 cut(s) | 1363 |
| VpaK11BI | GGWCC | 6 cut(s) | 156, 224, 955, 1087, 2204, 2359 |
| XapI | RAATTY | 5 cut(s) | 179, 1526, 1728, 1989, 2623 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1136 |
| XhoI | CTCGAG | 1 cut(s) | 403 |
| XmiI | GTMKAC | 1 cut(s) | 2452 |
| XmnI | GAANNNNTTC | 2 cut(s) | 386, 1594 |
| XspI | CTAG | 1 cut(s) | 680 |